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Purified Phlorizin from DocynIa Indica (Wall.) Decne by HSCCC, Compared with Whole Extract, Phlorizin and Non-Phlorizin Fragment Ameliorate Obesity, Insulin Resistance, and Improves Intestinal Barrier Function in High-Fat-Diet-Fed Mice

1
College of Life Sciences, Sichuan Normal University, Longquan, Chengdu 610101, China
2
State Key Laboratory for Quality Research in Chinese Medicine, University of Macau, Macao SAR, China
3
College of Life Sciences, Sichuan University, Wuhou, Chengdu 610065, China
4
School of Medicine and Nursing, Chengdu University, Longquan, Chengdu 610106, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2018, 23(10), 2701; https://doi.org/10.3390/molecules23102701
Received: 26 September 2018 / Revised: 16 October 2018 / Accepted: 17 October 2018 / Published: 19 October 2018
(This article belongs to the Special Issue Natural Bioactives in Anti-Obesity Therapy)
Natural products generally contain complex and multiple bioactive compounds that are responsible for the effects on health through complicated synergistic and/or suppressive actions. As an important raw material of local ethnic minority tea, ethnomedicines and food supplements in southwestern areas of China, Docynia indica (Wall.) Decne (DID) mainly consists of phlorizin (PHZ), which is the main active component. In this study, the holistic activities and the interactions of components of PHZ, non-phlorizin (NP) in the DID extract (DIDE) were evaluated. A rapid and effective high-speed counter-current chromatography (HSCCC) was performed to knock out PHZ from DIDE and the purity of PHZ was 96.01% determined by HPLC, with a recovery rate of 96.76%. After 13 weeks of treatment course in a high-fat diet (HFD)-induced obese mice model, the results revealed that the DIDE and PHZ significantly decreased weight gain, blood lipid levels, hyperplasia of adipocytes and alleviated inflammation (p < 0.05). Both DIDE and PHZ improves insulin resistance (p < 0.001). Meanwhile, the intestinal barrier function was improved compared to HFD group, through the determination of serum lipopolysaccharides (LPS), glucagon-likepeptide-2 (GLP-2) and hematoxylin-eosin staining of jejunum. Interestingly, after NP treatment, the metabolic syndrome of the HFD-induced obesity appeared to have a similar improvement. All the experiments showed that there is a synergistic weakening phenomenon when PHZ and NP interact with each other in the mixed state. In conclusion, for the PHZ and NP showing a good effect on anti-obesity, anti-inflammation, and intestinal barrier function, DIDE could be a good source of functional food to prevent obesity. View Full-Text
Keywords: Docynia indica (Wall.) Decne; Phlorizin; Anti-obesity; Intestinal barrier; Interaction; HSCCC Docynia indica (Wall.) Decne; Phlorizin; Anti-obesity; Intestinal barrier; Interaction; HSCCC
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Zhang, X.-Y.; Yi, K.; Chen, J.; Li, R.-P.; Xie, J.; Jin, Y.; Mei, X.-R.; Li, Y.-J.; Liu, G.; Wang, Z.-G. Purified Phlorizin from DocynIa Indica (Wall.) Decne by HSCCC, Compared with Whole Extract, Phlorizin and Non-Phlorizin Fragment Ameliorate Obesity, Insulin Resistance, and Improves Intestinal Barrier Function in High-Fat-Diet-Fed Mice. Molecules 2018, 23, 2701.

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