Curcumin Attenuates on Carbon Tetrachloride-Induced Acute Liver Injury in Mice via Modulation of the Nrf2/HO-1 and TGF-β1/Smad3 Pathway
Abstract
:1. Introduction
2. Results
2.1. Curcumin Ameliorates CCl4-Induced Acute Liver Injury in Mice
2.2. Curcumin Ameliorates CCl4-Induced Oxidative Stress in Liver Tissue
2.3. Curcumin Ameliorates CCl4-Induced Inflammatory Response in Livers of Mice
2.4. Curcumin Down-Regulates the Activities of Caspase-9 and -3
2.5. Curcumin Up-Regulates Nrf2 Expression and Down-Regulates the Expressions of TGF-β1 and Smad3 Proteins
2.6. Curcumin Down-Regulates the Expressions of TGF-β and Smad3 mRNAs and Up-Regulates the Expressions of Nrf2 and HO-1 mRNAs
2.7. Inhibition of HO-1 Abolished the Protective Effect of Curcumin on CCl4-Induced Liver Injury
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Animal Treatment
4.3. Measurement of Serum Alanine Transaminase (ALT) and Aspartate Transaminase (AST)
4.4. Histological Examination of Liver Damage
4.5. Measurement of Activities of Superoxide Dismutase (SOD), Catalase (CAT) and Levels of Malondialdehyde (MDA) and Glutathione (GSH)
4.6. Measurement of Caspase-9, -3 and HO-1 Activities
4.7. Measurement of TNF-α, IL-1β, IL-6 and TGF-β1 Levels
4.8. Western Blotting
4.9. RNA Extraction and Real-Time Quantitative Polymerase Chain Reaction (PCR)
4.10. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
Treatment Group | ||||||
---|---|---|---|---|---|---|
Biomarker | Control | Cur 200 | CCl4 | Cur 50 + CCl4 | Cur 100 + CCl4 | Cur 200 + CCl4 |
MDA (mmol/mg protein) | 1.84 ± 0.22 | 1.77 ± 0.25 | 2.79 ± 0.31 ** | 2.54 ± 0.37 # | 2.41 ± 0.18 # | 2.14 ± 0.39 ## |
SOD (U/mg protein) | 110.2 ± 24.20 | 116.4 ± 13.94 | 78.4 ± 10.5 ** | 84.6 ± 15.1 | 94.3 ± 12.1 ## | 99.7 ± 17.2 ## |
CAT (U/mgprotein) | 130.7 ± 15.8 | 138.4 ± 21.4 | 86.5 ± 15.1 ** | 98.1 ± 10.6 # | 106.7 ± 20.1 # | 118.2 ± 17.8 ## |
GSH (mmol/mg protein) | 57.1 ± 6.7 | 62.6 ± 8.1 | 38.2 ± 9.4 ** | 43.3 ± 7.8 | 46.8 ± 10.3 # | 50.4 ± 9.6 # |
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Peng, X.; Dai, C.; Liu, Q.; Li, J.; Qiu, J. Curcumin Attenuates on Carbon Tetrachloride-Induced Acute Liver Injury in Mice via Modulation of the Nrf2/HO-1 and TGF-β1/Smad3 Pathway. Molecules 2018, 23, 215. https://doi.org/10.3390/molecules23010215
Peng X, Dai C, Liu Q, Li J, Qiu J. Curcumin Attenuates on Carbon Tetrachloride-Induced Acute Liver Injury in Mice via Modulation of the Nrf2/HO-1 and TGF-β1/Smad3 Pathway. Molecules. 2018; 23(1):215. https://doi.org/10.3390/molecules23010215
Chicago/Turabian StylePeng, Xinyan, Chongshan Dai, Quanwen Liu, Junke Li, and Jingru Qiu. 2018. "Curcumin Attenuates on Carbon Tetrachloride-Induced Acute Liver Injury in Mice via Modulation of the Nrf2/HO-1 and TGF-β1/Smad3 Pathway" Molecules 23, no. 1: 215. https://doi.org/10.3390/molecules23010215
APA StylePeng, X., Dai, C., Liu, Q., Li, J., & Qiu, J. (2018). Curcumin Attenuates on Carbon Tetrachloride-Induced Acute Liver Injury in Mice via Modulation of the Nrf2/HO-1 and TGF-β1/Smad3 Pathway. Molecules, 23(1), 215. https://doi.org/10.3390/molecules23010215