Next Article in Journal
Phenylpropionamides, Piperidine, and Phenolic Derivatives from the Fruit of Ailanthus altissima
Previous Article in Journal
Plants of the Genus Zingiber as a Source of Bioactive Phytochemicals: From Tradition to Pharmacy
Open AccessArticle

Using the SPE and Micro-HPLC-MS/MS Method for the Analysis of Betalains in Rat Plasma after Red Beet Administration

Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Tuwima 10, 10-748 Olsztyn, Poland
*
Author to whom correspondence should be addressed.
Molecules 2017, 22(12), 2137; https://doi.org/10.3390/molecules22122137
Received: 7 November 2017 / Revised: 1 December 2017 / Accepted: 2 December 2017 / Published: 4 December 2017
(This article belongs to the Section Metabolites)
The objective of this study was to develop a simple and reproducible method for the qualitative and quantitative analysis of betalains in plasma samples, based on Solid Phase Extraction (SPE) and micro-high performance liquid chromatography coupled with mass spectrometry (micro-HPLC-MS/MS). The eight betalain compounds detected and quantified were characterized in the fortified rat blood plasma samples. The developed method showed a good coefficient of determination (R2 = 0.999), good recovery, precision, and appropriate limits of detection (LOD) and quantification (LOQ) for these compounds. Application of this method for the treatment of rat plasma samples collected after the betalain preparation administration, for the first time, revealed the presence of native betalains and their metabolites in plasma samples. Moreover, among them, betanin (2.14 ± 0.06 µmol/L) and isobetanin (3.28 ± 0.04 µmol/L) were found at the highest concentration. The results indicated that the combination of an SPE method with a micro-HPLC-MS/MS analysis may be successfully applied for the determination of betalains in the blood plasma. View Full-Text
Keywords: betalains; plasma; solid phase extraction (SPE); micro-HPLC-MS/MS; validation method betalains; plasma; solid phase extraction (SPE); micro-HPLC-MS/MS; validation method
Show Figures

Graphical abstract

MDPI and ACS Style

Sawicki, T.; Juśkiewicz, J.; Wiczkowski, W. Using the SPE and Micro-HPLC-MS/MS Method for the Analysis of Betalains in Rat Plasma after Red Beet Administration. Molecules 2017, 22, 2137.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop