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Molecules 2017, 22(10), 1583;

Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells

School of Pharmacy, Sungkyunkwan University, Suwon 16419, Korea
College of Korean Medicine, Gachon University, Seongnam 13120, Korea
Institute of Green Bio Science & Technology, Seoul National University, Pyeong Chang 232-916, Korea
College of Pharmacy, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Korea
Department of Food and Nutrition, Gachon University, Seongnam 13120, Korea
Author to whom correspondence should be addressed.
Received: 25 August 2017 / Revised: 20 September 2017 / Accepted: 20 September 2017 / Published: 21 September 2017
(This article belongs to the Collection Bioactive Compounds)
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The edible fungus Phellinus baumii Pilat (Hymenochaetaceae) has been used in Korean traditional medicines for strengthening health and prolonging life. An extract of the fruiting bodies of P. baumii was subjected to bioassay-guided fractionation based on its anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The resulting fractions were chemically investigated, leading to isolation of three phenolic compounds (13), a sesquiterpene (4), two steroids (56), a fatty acid (7), and a cerebroside (8). Spectroscopic analyses including 1D and 2D NMR spectroscopy and LC/MS were used to determine their chemical structures. Compounds 2, 4, 5, 7 and 8 were identified in P. baumii for the first time. Since all compounds were isolated from active fractions with anti-inflammatory activity, their ability to inhibit LPS-stimulated nitric oxide (NO) production in RAW264.7 cells were evaluated in vitro. Compounds 1, 2, 3, 5 and 7 inhibited LPS-stimulated NO production, and compounds 13 had IC50 values <10 μM. Treatment of LPS-stimulated RAW264.7 cells with compounds 13 inhibited phosphorylation of IKKα and IκBα. In addition, treatment of compounds 13 reduced LPS-induced increases of nuclear factor-kappa B (NF-κB) p65, iNOS and COX-2 protein expressions. Collectively, compounds 13 inhibited NF-κB-dependent inflammation in RAW264.7 cells. Thus, P. baumii is a potential source of natural anti-inflammatory agents, and active compounds 13 could be promising lead compounds for the development of novel anti-inflammatory agents. View Full-Text
Keywords: Phellinus baumii; Hymenochaetaceae; bioactivity-guided isolation; anti-inflammation; NO; NF-kappaB Phellinus baumii; Hymenochaetaceae; bioactivity-guided isolation; anti-inflammation; NO; NF-kappaB

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Lee, S.; Lee, D.; Jang, T.S.; Kang, K.S.; Nam, J.-W.; Lee, H.-J.; Kim, K.H. Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells. Molecules 2017, 22, 1583.

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