Next Article in Journal
Coverage Dependent Variation of the Adsorption Structure of 2-Thiophenecarboxaldehyde on the Ge(100)-2 × 1 Reconstructed Surface
Previous Article in Journal
GABAB-Agonistic Activity of Certain Baclofen Homologues
Article Menu

Export Article

Open AccessArticle
Molecules 2013, 18(9), 10285-10300;

Protective Effects of Alkaloid Compounds from Nelumbinis Plumula on tert-Butyl Hydroperoxide-Induced Oxidative Stress

College of Food Science and technology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China
Author to whom correspondence should be addressed.
Received: 11 July 2013 / Revised: 12 August 2013 / Accepted: 16 August 2013 / Published: 26 August 2013
Full-Text   |   PDF [266 KB, uploaded 18 June 2014]   |  


This study was conducted to investigate the effect of Nelumbinis Plumula total alkaloid (NPA) and its main alkaloid components on oxidative stress induced by tert-butyl hydroperoxide (t-BHP) in the human hepatocellular HepG2 cell line. According to HPLC analysis, several major alkaloid compounds such as liensinine, isoliensinine and neferine were present in NPA. The cytotoxic effects in 0.55 mM t-BHP-induced HepG2 cells were significantly inhibited by NPA and the major compound in NPA, neferine, showed the strongest activities. The protective effect of neferine against oxidative stress induced by t-BHP may be associated with decreased ROS formation, TBARS generation, LDH release and increased GSH levels, suggesting their involvement of the cytoprotective on oxidative stress. The effects were comparable with quercetin, which was used as positive control. Overall, total alkaloid and alkaloid compounds from Nelumbinis Plumula displayed a significant cytoprotective effect against oxidative stress. Further study is needed to elucidate the relationship between the chemical structures of the components in NPA and their protective effect on oxidative stress. View Full-Text
Keywords: cytoprotection; alkaloid; oxidative stress; Nelumbinis Plumula cytoprotection; alkaloid; oxidative stress; Nelumbinis Plumula

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Xie, Y.; Zhang, Y.; Zhang, L.-T.; Zeng, S.-X.; Guo, Z.-B.; Zheng, B.-D. Protective Effects of Alkaloid Compounds from Nelumbinis Plumula on tert-Butyl Hydroperoxide-Induced Oxidative Stress. Molecules 2013, 18, 10285-10300.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top