Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chromatographic Action of Extract from P. adiposa
2.2. Identification of the Extract PB3
2.3. Effect of PB3 on mRNA Expression of SOD
2.4. Effect of PB3 on mRNA Expression of Immunomodulatory Cytokines
3. Experimental
3.1. Reagents
3.2. Animals
3.3. Sample Preparation and Isolation of Adenosine
3.4. Real-Time PCR
4. Conclusions
Acknowledgments
References
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Sample Availability: Samples of the compound PB3 are available from the authors. |
Gene | ΔCt * | ΔΔCt ** | 2−ΔΔCt |
---|---|---|---|
IL-2 | 9.43 ± 0.15 | 1.03 | 0.490 |
IL-6 | 7.60 ± 0.19 | 0.81 | 0.569 |
IL-10 | 8.05 ± 0.05 | −0.57 | 1.481 |
IFN-γ | 7.81 ± 0.17 | 0.45 | 0.734 |
SOD | 3.95 ± 0.17 | −0.70 | 1.621 |
Gene | Sequence | Product | |
---|---|---|---|
SOD | upstream: | 5'-GCAGGGAACCATCCACTT-3' | 215 bp |
downstream: | 5'-TGCCCAGGTCTCCAACAT-3' | ||
IL-2 | upstream: | 5'-TGAACTTGGACCTCTGCG-3' | 220 bp |
downstream: | 5'-AGGGCTTGTTGAGATGATGC-3' | ||
IL-6 | upstream: | 5'-TTCTTGGGACTGATGCTG-3' | 380 bp |
downstream: | 5'-CTGGCTTTGTCTTTCTTGTT-3' | ||
IL-10 | upstream: | 5'-ACCAAAGCCACAAAGCAG-3' | 249 bp |
downstream: | 5'-GGAGTCGGTTAGCAGTATG-3' | ||
IFN-r | upstream: | 5'-TGAGACAATGAACGCTAC-3' | 142 bp |
downstream: | 5'-TTCCACATCTATGCCACT-3' | ||
GAPDH | upstream: | 5'-TCAACGGCACAGTCAAGG-3' | 470 bp |
downstream: | 5'-ACCAGTGGATGCAGGGAT-3' |
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Wang, C.R.; Qiao, W.T.; Zhang, Y.N.; Liu, F. Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines. Molecules 2013, 18, 1775-1782. https://doi.org/10.3390/molecules18021775
Wang CR, Qiao WT, Zhang YN, Liu F. Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines. Molecules. 2013; 18(2):1775-1782. https://doi.org/10.3390/molecules18021775
Chicago/Turabian StyleWang, Chang Rong, Wen Tao Qiao, Ye Ni Zhang, and Fang Liu. 2013. "Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines" Molecules 18, no. 2: 1775-1782. https://doi.org/10.3390/molecules18021775