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Antioxidant Activities and Polyphenolic Contents of Three Selected Micromeria Species from Croatia

1
Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Marulićev trg 20, HR-10000 Zagreb, Croatia
2
Department of Botany and Botanical Garden, Faculty of Science, University of Zagreb, Marulićev trg 20, HR-10000 Zagreb, Croatia
3
Institute of Laboratory Medicine, Medical School, University of Pécs, Ifjúság útja13, H-7624 Pécs, Hungary
4
Department of Medical Biochemistry and Haematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Domagojeva 2, HR-10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Molecules 2011, 16(2), 1454-1470; https://doi.org/10.3390/molecules16021454
Received: 14 January 2011 / Revised: 28 January 2011 / Accepted: 6 February 2011 / Published: 10 February 2011
(This article belongs to the Section Natural Products Chemistry)
Antioxidant activities of three selected Micromeria species growing in Croatia (M. croatica, M. juliana and M. thymifolia) were evaluated using five different antioxidant assays, in comparison with plant polyphenolic constituents and reference antioxidants. All studied ethanolic extracts exhibited considerable activity to scavenge DPPH and hydroxyl free radicals, reducing power, iron chelating ability and total antioxidant capacity in the order: M. croatica > M. juliana > M. thymifolia. Total polyphenol (9.69–13.66%), phenolic acid (5.26–6.84%), flavonoid (0.01–0.09%) and tannin (3.07–6.48%) contents in dried plant samples were determined spectrophotometrically. A strong positive correlation between antioxidant activities and contents of phenolic acids and tannins was found, indicating their responsibility for effectiveness of tested plants. Our findings established Micromeria species as a rich source of antioxidant polyphenols, especially the endemic M. croatica. View Full-Text
Keywords: Micromeria croatica; Micromeria juliana; Micromeria thymifolia; antioxidant activity; polyphenols; rosmarinic acid Micromeria croatica; Micromeria juliana; Micromeria thymifolia; antioxidant activity; polyphenols; rosmarinic acid
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Vladimir-Knežević, S.; Blažeković, B.; Bival Štefan, M.; Alegro, A.; Kőszegi, T.; Petrik, J. Antioxidant Activities and Polyphenolic Contents of Three Selected Micromeria Species from Croatia. Molecules 2011, 16, 1454-1470.

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