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Advances in Chromatographic Separation
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Department of Basic and Applied Sciences, University of Basilicata, Viale dell’Ateneo Lucano, 10-85100 Potenza, Italy
Department of Basic and Applied Sciences, University of Basilicata, Viale dell’Ateneo Lucano, 10-85100 Potenza, Italy
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Advances in Chromatographic Separation

Abstract submission deadline
28 February 2026
Manuscript submission deadline
30 April 2026
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2011

Topic Information

Dear Colleagues,

To the present day, chromatography has played a central role in separation science, as it represents one of the most widely used techniques in various fields such as food safety, drug discovery, and environmental monitoring. Chromatographic separation techniques have been implemented to detect a broad range of analytes using various retention mechanisms thanks to their remarkable advantages including their high sample loading capacity, exceptional efficiency, and adjustable selectivity. This Special Issue is focused on progress in chromatographic methods, based on high-performance liquid chromatography, gas chromatography, and supercritical fluid chromatography, for the separation of analytes belonging to food, biological, and environmental matrices.

Dr. Maria Assunta Acquavia
Dr. Giuliana Bianco
Topic Editors

Keywords

  • chromatography
  • separation
  • LC–MS
  • GC–MS
  • LC–MS/MS

Participating Journals

Journal Name Impact Factor CiteScore Launched Year First Decision (median) APC
Analytica
analytica 		- 1.8 2020 17.9 Days CHF 1000 Submit
International Journal of Molecular Sciences
ijms 		4.9 8.1 2000 16.8 Days CHF 2900 Submit
Molecules
molecules 		4.2 7.4 1996 15.1 Days CHF 2700 Submit
Processes
processes 		2.8 5.1 2013 14.9 Days CHF 2400 Submit
Separations
separations 		2.5 3.0 2014 15.1 Days CHF 2600 Submit

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Published Papers (3 papers)

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26 pages, 12159 KiB  
Review
Research Progress on the Development and Application of Cyclodextrin-Based Chromatographic Stationary Phases
by Wenlanqi Du, Jia Jia, Yinghong Zhao, Ailing Ning and Rongfu Huang
Separations 2025, 12(4), 97; https://doi.org/10.3390/separations12040097 - 14 Apr 2025
Viewed by 210
Abstract
This review systematically summarizes the novel preparation methods of cyclodextrin-based chromatographic stationary phases and their applications for chiral recognition in separation techniques such as capillary gas chromatography and high-performance liquid chromatography. Aiming at the current situation that enantiomers of chiral compounds present significant [...] Read more.
This review systematically summarizes the novel preparation methods of cyclodextrin-based chromatographic stationary phases and their applications for chiral recognition in separation techniques such as capillary gas chromatography and high-performance liquid chromatography. Aiming at the current situation that enantiomers of chiral compounds present significant differences at the pharmacological, pharmacodynamic, and toxicological levels, the core value of chromatographic chiral separation technology in the field of drug discovery and development is emphasized. By analyzing the unique cavity structure and excellent stereoselective properties of cyclodextrins, the mechanism of their action as a chromatographic stationary phase was elaborated. Combined with the typical applications of different derivatized cyclodextrin stationary phases in drug analysis, environmental testing, and biological samples, the value and potential of cyclodextrin stationary phases in stereoisomer separation are systematically demonstrated. Full article
(This article belongs to the Topic Advances in Chromatographic Separation)
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14 pages, 1531 KiB  
Article
Conjugation of Short-Chain Fatty Acids to Bicyclic-Amines for Analysis by Liquid Chromatography Tandem Mass Spectroscopy
by Daniel N. Darlington
Molecules 2025, 30(2), 341; https://doi.org/10.3390/molecules30020341 - 16 Jan 2025
Viewed by 594
Abstract
Conjugation of short-chain fatty acids (SDFAs) to amines containing ring structures allows for better measurement by liquid chromatography tandem mass spectroscopy (LC-MS/MS). However, collision-induced dissociation (CID) results in breaking the conjugate back to the original SCFA and amine. We therefore set out to [...] Read more.
Conjugation of short-chain fatty acids (SDFAs) to amines containing ring structures allows for better measurement by liquid chromatography tandem mass spectroscopy (LC-MS/MS). However, collision-induced dissociation (CID) results in breaking the conjugate back to the original SCFA and amine. We therefore set out to find an amine that would remain on the SCFA after CID and create a unique daughter for selectivity of measurement. Of twenty-seven amines with ring structures, we found four that contain bicycle-type structures (two rings connected by a carbon) with nitrogen in the second ring. CID removes the second ring at the nitrogen, leaving the first ring on the daughter. Of the four amines, 4-(pyrrolidine-1-ylmethyl) benzylamine (4PyBA) showed the strongest conjugation. Conjugation of 4PyBA to SCFA (C3–C6), their isomers and their phenylated versions (and isomers) resulted in good chromatographic peaks and separation. CID resulted in unique daughters that allowed for selectivity of measurement. Using this method, standard curves were generated that show good linearity (r2 > 0.99) in the nM and μM range with lower limits of detection between 40 and 229 nM for a 10 μL sample. Finally, we used this method to measure SCFA in plasma, liver, platelets, and red blood cells, demonstrating its use in biological systems. Because SCFAs are an index of microbiome diversity in the gastrointestinal track, this method will allow us to study changes in SCFAs and the microbiome in pathologic conditions including trauma, hemorrhage, and sepsis. Full article
(This article belongs to the Topic Advances in Chromatographic Separation)
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19 pages, 4605 KiB  
Article
Estimation and Uncertainty Quantification of Solvent Strength Parameters in Gradient Elution of Chromatography Using Sequential Monte Carlo Method
by Ziting Yuan, Kensuke Suzuki, Yota Yamamoto, Tomoyuki Yajima and Yoshiaki Kawajiri
Processes 2025, 13(1), 114; https://doi.org/10.3390/pr13010114 - 4 Jan 2025
Viewed by 735
Abstract
Gradient elution is a common method for the separation of complex components in chromatography. Strength-dependent models that describe the variation in chromatographic parameters, considering equilibrium and mass transfer under different solution strengths, are essential for the design and optimization of gradient elution. In [...] Read more.
Gradient elution is a common method for the separation of complex components in chromatography. Strength-dependent models that describe the variation in chromatographic parameters, considering equilibrium and mass transfer under different solution strengths, are essential for the design and optimization of gradient elution. In this study, we proposed several hypothetical models based on experimental data obtained under varying mobile phase ratios in the water–methanol system. The uncertainties in the parameters were quantified using the sequential Monte Carlo (SMC) method with Bayesian inference. Finally, we performed model selection to determine the most appropriate model, utilizing multiple sources of information criteria. Full article
(This article belongs to the Topic Advances in Chromatographic Separation)
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