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12 pages, 3860 KB  
Article
Correlation Analysis of BLTP1 (KIAA1109) and KIF27 Gene Polymorphisms with Wool Traits in Subo Merino Sheep
by Qingfa Yan, Sen Tang, Asma Anwar, Gvlnigar Amar, Yaqian Wang, Wenna Liu, Cuiling Wu and Xuefeng Fu
Genes 2026, 17(3), 295; https://doi.org/10.3390/genes17030295 - 28 Feb 2026
Viewed by 432
Abstract
Background/Objectives: The Subo Merino sheep is a high-quality fine-wool breed developed through progressive hybridization, characterized by high wool yield and excellent wool quality. This study is designed to investigate the effects of two gene polymorphisms in Subo Merino sheep on wool traits, [...] Read more.
Background/Objectives: The Subo Merino sheep is a high-quality fine-wool breed developed through progressive hybridization, characterized by high wool yield and excellent wool quality. This study is designed to investigate the effects of two gene polymorphisms in Subo Merino sheep on wool traits, thereby providing critical theoretical and technical support for the breeding of high-quality fine-wool sheep. Methods: In this study, 944 one-year-old Subo Merino sheep were genotyped for coding regions of the BLTP1 and KIF27 genes using the Fluidigm BioMark™ HD system. Association between SNP loci and wool traits was analyzed via the least squares means method in SAS 9.4. Protein–protein interaction networks were constructed using the STRING database, and protein structures before and after mutation were predicted with SOPMA and SWISS-MODEL. Results: The results revealed that BLTP1 gene identified a missense mutation site SNP1, which resulted in a nucleotide change c.812 (C > T) and an amino acid change p.Pro271Leu. KIF27 gene identified a missense mutation site SNP2, which resulted in a nucleotide change c.3896 (T > C) and an amino acid change p.Met1299Thr. Association analysis showed that SNP1 had a significant effect on wool crimp number (CN) and staple length (SL) (p < 0.05), while SNP2 significantly affected live weight after shearing (LWAS) (p < 0.05). Protein structure prediction showed that mutations at SNP1 and SNP2 primarily led to changes in α-helix, extended chain, and random coil structures. Conclusions: These results suggest that SNP1 in BLTP1 and SNP2 in KIF27 could serve as potential molecular markers for wool traits in Subo Merino sheep. This study provides theoretical support and candidate gene targets for molecular marker-assisted breeding, contributing to genetic improvement and efficient breeding of this fine-wool breed. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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37 pages, 1612 KB  
Systematic Review
Recent Advances in Biocomposite Materials Reinforced with Raw or Minimally Processed Wool: Fabrication Methods, Properties and Applications—A Systematic Review
by Carlos Ruiz-Díaz, Óscar Rodríguez-Alabanda, María M. Serrano-Baena and Guillermo Guerrero-Vacas
J. Compos. Sci. 2026, 10(2), 104; https://doi.org/10.3390/jcs10020104 - 16 Feb 2026
Viewed by 801
Abstract
Sheep wool is a keratin-based natural fiber increasingly explored as a low-impact reinforcement and multifunctional modifier in composites, enabling valorization of coarse or waste wool streams. This systematic review consolidates evidence on raw or minimally processed wool-reinforced composites across polymer matrices and mineral [...] Read more.
Sheep wool is a keratin-based natural fiber increasingly explored as a low-impact reinforcement and multifunctional modifier in composites, enabling valorization of coarse or waste wool streams. This systematic review consolidates evidence on raw or minimally processed wool-reinforced composites across polymer matrices and mineral binders. Following a registered protocol and Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020, Scopus and Web of Science were searched for English-language journal articles (2015–2025), yielding 44 included studies after screening. Evidence mapping shows polymers dominate (33/44; thermosets 19/44), while mineral binders account for 11/44. Wool is mainly used as short fibers (27/44), with woven (9/44) and nonwoven/felt (8/44) architectures appearing in laminates and insulation products. Because heterogeneity limits pooled meta-analysis, outcomes are synthesized using matched-control comparisons where available (27/44) and interpreted with a TRiC appraisal (Transparency, Reproducibility, and Credibility). Mechanical effects are highly conditional: gains in impact/energy absorption and occasional tensile/flexural stress improvements coexist with frequent losses linked to dispersion, wetting/impregnation and void sensitivity. Functional trends are similarly system-dependent, with promising but uneven evidence for acoustic performance, variable thermal conductivity shifts, and formulation-driven fire behavior. Moisture uptake and durability emerge as principal translation bottlenecks, motivating minimum reporting and design practices to improve comparability and application readiness. Full article
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15 pages, 1406 KB  
Article
Acoustic Attenuation Performance of Casing Stiffness Relative to Insulation Thickness in Compact Air Handling Units
by Titus Otniel Joldos and Florin Ioan Bode
Appl. Sci. 2026, 16(4), 1803; https://doi.org/10.3390/app16041803 - 11 Feb 2026
Viewed by 366
Abstract
The current global context, characterized by climate change and increased indoor occupancy, has necessitated prolonged daily operating hours for ventilation systems. Coupled with rising living standards, these factors have elevated occupants’ expectations for Indoor Environmental Quality (IEQ), driving a demand for quieter equipment [...] Read more.
The current global context, characterized by climate change and increased indoor occupancy, has necessitated prolonged daily operating hours for ventilation systems. Coupled with rising living standards, these factors have elevated occupants’ expectations for Indoor Environmental Quality (IEQ), driving a demand for quieter equipment which is a significant challenge for HVAC engineering. This study evaluates the acoustic attenuation performance of various casing constructions to quantify the impact of sheet metal stiffness compared to insulation thickness. Experimental measurements of the Radiated Sound Power Level (LwA) were conducted on a heat recovery unit across octave bands from 63 Hz to 16,000 Hz, ensuring a measurement uncertainty within ±0.5 dB as per ISO 3741 precision requirements. The methodology involved testing multiple enclosed configurations against a reference open-top unit, varying mineral wool insulation thickness from 40 mm to 100 mm (with optional 25 mm linings) and inner sheet metal thickness between 0.8 mm and 2.0 mm. The results indicate that enclosing the unit significantly reduced radiated sound power levels compared to the exposed reference. While the standard configuration with 50 mm insulation yielded 49.8 dBA, modifying the casing structure generated superior attenuation. Notably, a configuration utilizing a 2.0 mm inner sheet resulted in a radiated sound power level of 46.9 dBA, a result found to be statistically significant (p < 0.05) when compared to the baseline. This performance is statistically comparable to the 46.7 dBA recorded for the maximum insulation assembly, confirming the validity of structural stiffening as an equivalent alternative to bulk insulation. Consequently, the increased panel stiffness achieved approximately 94% of the attenuation efficiency provided by the thickest insulation option. The data demonstrates that increasing panel stiffness effectively reduces transmission, offering performance levels comparable to significantly thicker insulation layers. The study concludes that optimizing casing stiffness represents a superior strategy for noise control in high-density residential applications, as it decouples acoustic performance from the unit’s external dimensions, offering a high-attenuation solution that preserves a compact spatial footprint. Full article
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15 pages, 1784 KB  
Article
Prevention of Hair Heat Damage via Thermoresponsive Organic Silicon-Modified Keratin
by Chaohai Li, Jinhua Li, Kuan Chang and Jing Wang
Molecules 2026, 31(3), 521; https://doi.org/10.3390/molecules31030521 - 2 Feb 2026
Cited by 1 | Viewed by 945
Abstract
Heat damage is a common phenomenon that often occurs when drying and straightening hair. After heat damage, the hydrophobic barrier on the hair’s surface becomes disrupted, thereby altering the hair’s hydrophilicity. Meanwhile, during the process of heat damage, the rupture of the hair’s [...] Read more.
Heat damage is a common phenomenon that often occurs when drying and straightening hair. After heat damage, the hydrophobic barrier on the hair’s surface becomes disrupted, thereby altering the hair’s hydrophilicity. Meanwhile, during the process of heat damage, the rupture of the hair’s cuticles causes the hair to become dry and rough, with a decrease in gloss and a decline in mechanical properties. This study utilized epoxy silane and hydrolyzed wool keratin to synthesize a thermally responsive organic silicon-modified keratin (OSK) to prevent hair from heat damage. OSK was synthesized from epoxy silane and hydrolyzed keratin, with yield determined by quantifying free amino groups. Its hair-care performance was evaluated through assessments of hair surface morphology, mechanical properties, and optical gloss, and by combing test and contact angle measurements. Mechanisms underlying surface hydrophobicity and hair scale protection were investigated using FTIR, XPS, and DSC. Specific performance parameters were evaluated using a single-fiber strength tester and a multifunctional hair-testing instrument. FTIR confirmed successful covalent grafting, with synthesis optimized to a 90.67% yield. OSK forms a protective film on hair surfaces, verified by SEM, XPS, and TEM, restoring damaged hair hydrophobicity to a 117° contact angle and enhancing thermal protection to 136° upon heating. Beyond hydrophobic-barrier restoration, OSK improved hair gloss by 30.26% and reduced frizz by 39.33%, while restoring the key performance of virgin hair. It also provides exceptional water-repellency protection and sensory enhancement. Under thermal stress, the protective film mechanically increased tensile strength by 6.58% and yield zone tensile force by 4.65%. This article demonstrates that OSK is an effective heat-sensitive agent. When damaged by heat sources such as hair dryers, it will form a protective film on the surface of the hair, thereby protecting the surface properties of the hair. Full article
(This article belongs to the Special Issue Functional Molecules as Novel Cosmetic Ingredients, 2nd Edition)
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21 pages, 4522 KB  
Article
Transcriptomic Exploration of Muscle Development and Fat Deposition Trait Diversity in Selected Indian Sheep Breeds: Implications for Meat Quality and Yield
by Navya Pothireddy, Mangalathu Rajan Vishnuraj, Kappala Vijaya Rachel, Peddapuram Baswa Reddy, Prashantha Chowdadenahalli Nagaraja, Ajay Ganesan, Shiva Shankar Kanneboyina, Krishnachaithanya Indiradevi and Sukhadeo Baliram Barbuddhe
Animals 2026, 16(3), 452; https://doi.org/10.3390/ani16030452 - 1 Feb 2026
Viewed by 1192
Abstract
Sheep’s meat production and quality are influenced by genetic and physiological factors that affect muscle development, growth, and fat deposition metabolism. However, the breed-specific transcriptional landscapes driving these traits in Indian sheep breeds, especially in Nellore (meat-type) and Deccani (wool-meat type) breeds are [...] Read more.
Sheep’s meat production and quality are influenced by genetic and physiological factors that affect muscle development, growth, and fat deposition metabolism. However, the breed-specific transcriptional landscapes driving these traits in Indian sheep breeds, especially in Nellore (meat-type) and Deccani (wool-meat type) breeds are remain unexplored. Therefore, this study aimed to investigate the differences in muscle growth and fat deposition between Nellore and Deccani breeds by integrating transcriptomic profiling, carcass characteristics, and histological analysis of longissimus dorsi muscle and liver tissues. Carcass assessment revealed higher Hot Carcass Weight (HCW), Cold Carcass Weight (CCW), Hot Carcass Yield (HCY) and Cold Carcass Yield (CCY), and larger myofibrillar cross-sectional area (p < 0.05), indicating enhanced musculature, which was observed in Nellore. Deccani showed elevated Intramuscular Fat (IMF) deposition (p < 0.05), indicating improved meat flavour/juiciness. Transcriptomic profiling revealed several Differentially Expressed Genes (DEGs) associated with meat quality and quantity traits. In Nellore, the genes WFIKKN2, FGFRL1, FKBP4, and IRF1 were upregulated, while the gene TAS1R2 was downregulated, leading to enhanced muscle development, superior carcass traits, thermotolerance, and immunity. While Deccani showed higher expression of lipid metabolism genes PLA2G4F, ACSL1, ACOX1, CPT1A, and PLIN1, which are linked to higher IMF content. Functional enrichment analysis revealed 46 significantly enriched GO terms for the DEGs (p < 0.05), including oxidoreductase activity, muscle development, etc. These outcomes demonstrate novel genetic markers and key biological insights into the regulation of muscle development, thermotolerance, immunity, and IMF for future validation in Indian sheep breeds. Full article
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24 pages, 12672 KB  
Article
Research on the Thermal–Stress Coupling Effect and Fire Protection Structures of SHS Group Columns of Steel Structure Modular Units
by Jiadi Liu and Feiyan Gao
Buildings 2026, 16(3), 525; https://doi.org/10.3390/buildings16030525 - 28 Jan 2026
Viewed by 367
Abstract
Modular construction refers to the use of factory prefabricated integrated module units. The modular steel construction unit SHS (Square Hollow Section) group column is a structure composed of four independent steel column units. Due to its compositional characteristics with voids, the fire resistance [...] Read more.
Modular construction refers to the use of factory prefabricated integrated module units. The modular steel construction unit SHS (Square Hollow Section) group column is a structure composed of four independent steel column units. Due to its compositional characteristics with voids, the fire resistance performance differs from ordinary steel columns, necessitating specific study. This paper employed a sequentially coupled thermal–mechanical analysis to investigate this. The effectiveness of the simulation model was first validated by comparing the simulated time–temperature curves and fire resistance limits with experimental results. A parametric analysis was then conducted to evaluate the influence of various factors, including the load ratio, cavity spacing, insulation type, gypsum board thickness, slenderness ratio, steel yield strength, and inner panel type, on the fire resistance limit. The results show that when the gypsum board thickness increased from 10 mm to 30 mm, the fire resistance limit correspondingly increased by 126%, 120%, 130%, and 130% for load ratios of 0.4, 0.5, 0.6, and 0.7, respectively. When the steel yield strength increased from 235 MPa to 690 MPa, the fire resistance limit increased by 20%, 21%, 24%, and 43% for load ratios ranging from 0.4 to 0.7. For inner panels of Glass Fiber, Rock Wool, Mineral Wool, and Plasterboard, the corresponding fire resistance limit ratios for load ratios of 0.4 to 0.7 were 1:1.13:1.24:1.45, 1:1.14:1.23:1.46, 1:1.11:1.2:1.42, and 1:1.08:1.18:1.41, respectively. It can be found that the best way to increase the fire resistance of the modular column is to increase the thickness of the gypsum board. A simplified calculation formula for the fire resistance limit of SHS group columns was derived through regression analysis, and recommendations for fire protection design were proposed, providing valuable insights for the future design and application of SHS group columns in steel modular construction. Full article
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18 pages, 1505 KB  
Article
Assessment of the Possibility of Grinding Glass Mineral Wool Without the Addition of Abrasive Material for Use in Cement Materials
by Beata Łaźniewska-Piekarczyk and Dominik Smyczek
Sustainability 2026, 18(3), 1169; https://doi.org/10.3390/su18031169 - 23 Jan 2026
Cited by 1 | Viewed by 369
Abstract
Glass wool waste constitutes a rapidly increasing fraction of construction and demolition residues, yet it remains one of the most challenging insulation materials to recycle. Its non-combustible nature, extremely low bulk density, and high fibre elasticity preclude energy recovery and severely limit conventional [...] Read more.
Glass wool waste constitutes a rapidly increasing fraction of construction and demolition residues, yet it remains one of the most challenging insulation materials to recycle. Its non-combustible nature, extremely low bulk density, and high fibre elasticity preclude energy recovery and severely limit conventional mechanical recycling routes, resulting in long-term landfilling and loss of mineral resources. Converting glass wool waste into a fine mineral powder represents a potentially viable pathway for its integration into low-carbon construction materials, provided that industrial scalability, particle-size control, and chemical compatibility with cementitious binders are ensured. This study investigates the industrial-scale milling of end-of-life glass wool waste in a ventilated horizontal ball mill. It compares two grinding routes: a corundum-free route (BK) and an abrasive-assisted route (ZK) employing α-Al2O3 corundum to intensify fibre fragmentation. Particle size distribution was quantified by laser diffraction using cumulative and differential analyses, as well as characteristic diameters. The results confirm that abrasive-assisted milling significantly enhances fragmentation efficiency and reduces the coarse fibre fraction. However, the study demonstrates that this gain in fineness is inherently coupled with the incorporation of α-Al2O3 into the milled powder, introducing a chemically foreign crystalline phase that cannot be removed by post-processing. From a cement-oriented perspective, this contamination represents a critical limitation, as α-Al2O3 may interfere with hydration reactions, aluminate–sulfate equilibria, and microstructural development in Portland and calcium sulfoaluminate binders. In contrast, the corundum-free milling route yields a slightly coarser, chemically unmodified powder, offering improved process robustness, lower operational complexity, and greater compatibility with circular economy objectives. The study establishes that, for the circular reuse of fibrous insulation waste in cementitious systems, particle fineness alone is insufficient as an optimization criterion. Instead, the combined consideration of fineness, chemical purity, and binder compatibility governs the realistic and sustainable reuse potential of recycled glass wool powders. Full article
(This article belongs to the Section Sustainable Engineering and Science)
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21 pages, 2347 KB  
Article
Experimental Evaluation of Sheep Wool Fibres as Sustainable Reinforcement in Eco-Friendly Cement Mortars
by Carlos Ruiz-Díaz, Guillermo Guerrero-Vacas, Óscar Rodríguez-Alabanda, Manuel Cabrera and Julia Rosales
Materials 2026, 19(2), 427; https://doi.org/10.3390/ma19020427 - 22 Jan 2026
Cited by 2 | Viewed by 381
Abstract
Sheep wool is a low-value agricultural by-product with potential to contribute to more sustainable cementitious materials. This study investigates Segureña sheep wool fibres as reinforcement in cement mortars, comparing washed wool (W) and cement-encapsulated wool (E) at the same oven-dry raw wool dosages [...] Read more.
Sheep wool is a low-value agricultural by-product with potential to contribute to more sustainable cementitious materials. This study investigates Segureña sheep wool fibres as reinforcement in cement mortars, comparing washed wool (W) and cement-encapsulated wool (E) at the same oven-dry raw wool dosages (0.5, 1.0, and 3.0 g per batch), and benchmarking against polypropylene (PP) fibres. Flexural and compressive strength were evaluated at 1, 7, and 28 days, whereas apparent density, water absorption, and thermal conductivity were assessed at 28 days. An intermediate dosage (1.0 g per batch) provided the most favourable mechanical response, while the highest dosage (3.0 g per batch) reduced performance, plausibly due to dispersion limitations and void formation. At 28 days, W-1 reached 9.65 ± 0.50 MPa in flexure (very close to PP-1) and 59.70 ± 1.05 MPa in compression, exceeding PP-1 in compression. Wool incorporation also reduced apparent density and yielded an observed reduction in thermal conductivity of up to ~18% at the highest dosage (single specimen per series). Overall, optimally dosed washed wool can deliver competitive mechanical performance while improving thermal behaviour, supporting circular-economy valorisation of waste wool in eco-mortars. Full article
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19 pages, 4640 KB  
Article
Mechanical Performance of Wool-Reinforced Epoxy Composites: Tensile, Flexural, Compressive, and Impact Analysis
by Carlos Ruiz-Díaz, Guillermo Guerrero-Vacas and Óscar Rodríguez-Alabanda
Materials 2025, 18(23), 5391; https://doi.org/10.3390/ma18235391 - 29 Nov 2025
Cited by 4 | Viewed by 2177
Abstract
This study situates washed sheep-wool fibres as a sustainable reinforcement candidate for epoxy matrices and evaluates their mechanical response under tensile, flexural, compressive, and Charpy impact loading. The objective of this work is to assess whether short, washed sheep-wool fibres can function as [...] Read more.
This study situates washed sheep-wool fibres as a sustainable reinforcement candidate for epoxy matrices and evaluates their mechanical response under tensile, flexural, compressive, and Charpy impact loading. The objective of this work is to assess whether short, washed sheep-wool fibres can function as a sustainable reinforcement for epoxy matrices, and to identify optimal fibre length–content windows that improve mechanical behaviour for engineering applications. Moulded–machined specimens were produced with fibre lengths of 3, 6, and 10 mm and contents of 1.0–5.0 wt.%, depending on the test; neat epoxy served as the reference. In tension, selected formulations—particularly 10 mm/1.5 wt.%—showed simultaneous increases in ultimate stress and modulus relative to the neat resin, corresponding to gains of about 10% in ultimate tensile stress and 50% in tensile modulus, at the expense of ductility. In flexure, the modulus decreases by roughly 15–35% compared with the matrix, whereas configurations with 3–6 mm at 2.5–5 wt.% raise the fracture stress by about 35–45% and improve post-peak resistance. In compression, reinforcement markedly elevates yield stress, with increases of up to about 160% at 3 mm/2 wt.%, while the ultimate strain decreases moderately. In Charpy impact, all reinforced materials underperform the resin, with absorbed energy reduced by roughly 75–93% depending on fibre length and content, with 3 mm/1 wt.% being the least affected. A two-factor analysis of variance (ANOVA) indicates that fibre length primarily governs tensile and compressive behaviour, while fibre content dominates flexural and impact responses. Overall, the findings support wool fibres as a viable reinforcement when length and content are optimized, pointing to their use in non-structural to semi-structural industrial components such as interior panels, housings, casings, protective covers, and other parts where moderate tensile/compressive performance is sufficient and material sustainability is prioritised. Full article
(This article belongs to the Special Issue Advances in Polymer Blends and Composites—Second Edition)
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18 pages, 2265 KB  
Communication
Optimization of Mix Design for Lightweight Boards Based on GGBFS–Waste Rock Wool Using Response Surface Methodology
by Jun-Cheol Lee
Materials 2025, 18(23), 5376; https://doi.org/10.3390/ma18235376 - 28 Nov 2025
Viewed by 387
Abstract
This study aimed to establish the optimal mix proportions for eco-friendly lightweight boards based on Ground Granulated Blast-furnace Slag (GGBFS) and waste rock wool using Response Surface Methodology (RSM). The investigation focused on optimizing three key properties: flexural failure load (Y1 [...] Read more.
This study aimed to establish the optimal mix proportions for eco-friendly lightweight boards based on Ground Granulated Blast-furnace Slag (GGBFS) and waste rock wool using Response Surface Methodology (RSM). The investigation focused on optimizing three key properties: flexural failure load (Y1), moisture content (Y2), and specific gravity (Y3). ANOVA results identified Binder and Perlite as the most dominant and statistically significant factors, exhibiting critical conflicting effects necessary for balancing strength and lightweight goals. Wollastonite showed a non-linear effect on flexural strength, peaking at an intermediate level. A Response Optimization simulation, targeting a minimum flexural load of 400 N, moisture content of 2.0%, and specific gravity of 0.80, yielded an optimal mix proportion: Binder 52.12%, Perlite 48.45%, and Wollastonite 7.37%. This blend achieved a high Composite Desirability (D) of 0.8725. Experimental verification confirmed the model’s reliability. The measured flexural load (408.54 N) successfully exceeded the 400 N target, and all measured values exhibited a low error margin (under 7%) compared to the predicted values. This optimized mix proportion provides a reliable foundation for developing high-performance, sustainable lightweight construction materials. Full article
(This article belongs to the Section Construction and Building Materials)
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16 pages, 1111 KB  
Article
Two-Stage Machine Learning-Based GWAS for Wool Traits in Central Anatolian Merino Sheep
by Yunus Arzık, Mehmet Kizilaslan, Sedat Behrem, Simge Tütenk and Mehmet Ulaş Çınar
Agriculture 2025, 15(21), 2287; https://doi.org/10.3390/agriculture15212287 - 3 Nov 2025
Cited by 1 | Viewed by 991
Abstract
Wool traits such as fiber diameter, fiber length, and greasy fleece yield are economically significant characteristics in sheep breeding programs. Traditional genome-wide association studies (GWAS) have identified relevant genomic regions but often fail to capture the non-linear and polygenic architecture underlying these traits. [...] Read more.
Wool traits such as fiber diameter, fiber length, and greasy fleece yield are economically significant characteristics in sheep breeding programs. Traditional genome-wide association studies (GWAS) have identified relevant genomic regions but often fail to capture the non-linear and polygenic architecture underlying these traits. In this study, we implemented a two-stage machine learning (ML)-based GWAS framework to dissect the genetic basis of wool traits in Central Anatolian Merino sheep. Phenotypic records were collected from 228 animals, genotyped with the Illumina OvineSNP50 BeadChip. In the first stage, feature selection was conducted using LASSO, Ridge Regression, and Elastic Net, generating a consensus SNP panel per trait. In the second stage, association modeling with Random Forest and Support Vector Regression (SVR) identified the most predictive models (R2 up to 0.86). Candidate gene annotation highlighted biologically relevant loci: MTHFD2L and EPGN (folate metabolism and keratinocyte proliferation) for fiber diameter; COL5A2, COL3A1, ITFG1, and ELMO1 (extracellular matrix integrity and actin remodeling) for staple length; and FAP, DPP4, PLCH1, and NPTX1 (extracellular matrix remodeling, proteolysis, and sebaceous gland function) for greasy fleece yield. These findings demonstrate the utility of ML-enhanced GWAS pipelines in identifying biologically meaningful markers and propose novel targets for genomic selection strategies to improve wool quality and yield in indigenous sheep populations. Full article
(This article belongs to the Special Issue Genetic Diversity, Adaptation and Evolution of Livestock)
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14 pages, 1140 KB  
Article
Genome Selection for Fleece Traits in Inner Mongolia Cashmere Goats Based on GWAS Prior Marker Information
by Huanfeng Yao, Na Wang, Yu Li, Gang He, Jin Ning, Shuai Kang, Yongbin Liu, Jinquan Li, Qi Lv, Ruijun Wang, Yanjun Zhang, Rui Su and Zhiying Wang
Animals 2025, 15(21), 3184; https://doi.org/10.3390/ani15213184 - 31 Oct 2025
Cited by 1 | Viewed by 777 | Correction
Abstract
The Inner Mongolia Cashmere goat (IMCG) industry is a major contributor to global cashmere production, with fleece traits serving as key economic indicators that directly impact both income and the long-term sustainability of the industry. When genome-wide SNPs are used to estimate kinship [...] Read more.
The Inner Mongolia Cashmere goat (IMCG) industry is a major contributor to global cashmere production, with fleece traits serving as key economic indicators that directly impact both income and the long-term sustainability of the industry. When genome-wide SNPs are used to estimate kinship matrices, the traditional animal model implicitly assumes that all SNPs have the same effect-size distribution. However, in practice, there are differences in the genetic mechanisms and complexity of different traits. We conducted a genome-wide association study (GWAS) on 2299 IMCGs genotyped with 67,021 SNPs, which were obtained after imputation. The traits measured included cashmere yield (CY), wool length (WL), cashmere length (CL), and cashmere diameter (CD), with a total of 33,564 records collected. The top 5% to 20% of the significant SNPs from the GWAS were used as biological prior information. We then assigned proportional weights based on their contribution to the overall genetic variance and further integrated them with the remaining loci to construct a kinship relationship matrix for estimating genetic parameters and genomic breeding value. By incorporating prior marker information from the GWAS, it was found that the heritability estimates for CY, WL, CL, and CD were 0.26, 0.37, 0.09, and 0.35, respectively. For CY and CL, integrating the top 5% of prior SNP markers yielded the highest genomic prediction accuracies of 0.742 and 0.673, representing improvements of 16.67% and 19.75% over models that did not utilize prior information. In contrast, for WL and CD, the highest accuracies of 0.851 and 0.780 were achieved by integrating the top 10% of prior SNP markers, reflecting improvements of 9.81% and 10.14%, respectively. Compared with the conventional GBLUP method, this method of integrating GWAS-derived prior markers for genomic genetic evaluation can significantly improve the accuracy of genomic prediction for fleece traits in IMCGs. This approach facilitates accurate selection for fleece traits in IMCGs, enabling accelerated genetic progress through long-term breeding programs. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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19 pages, 2768 KB  
Article
Supercritical CO2 Extraction of Phoenix Dancong Tea Oil: Process Optimization and Fragrance Retention on Textiles
by Fanlin Zhou, Manus Kaewboucha and Chalisa Apiwathnasorn
Processes 2025, 13(11), 3503; https://doi.org/10.3390/pr13113503 - 31 Oct 2025
Viewed by 1410
Abstract
Phoenix Dancong tea essential oil possesses unique aroma characteristics and bioactivities, offering broad application potential in the food, pharmaceutical, and daily chemical fields. To achieve efficient extraction and expand its use in functional textiles, supercritical CO2 (SC-CO2) extraction was employed [...] Read more.
Phoenix Dancong tea essential oil possesses unique aroma characteristics and bioactivities, offering broad application potential in the food, pharmaceutical, and daily chemical fields. To achieve efficient extraction and expand its use in functional textiles, supercritical CO2 (SC-CO2) extraction was employed to optimize the extraction process of Phoenix Dancong tea essential oil. Based on single-factor experiments, the optimal extraction conditions were determined as follows: pressure of 25 MPa, temperature of 50 °C, CO2 flow rate of 8 L/h, and extraction time of 3 h, resulting in an essential oil yield of 1.12%. Response surface methodology (RSM) revealed that the experimental data fit the regression model well (R2 = 95.49%, R2Adj = 89.69%). Furthermore, the extracted essential oil was blade-coating to cotton, nylon, polyester, and wool fabrics to evaluate its aroma retention performance. Results indicated that cotton fibers exhibited the best absorption and sustained fragrance retention, maintaining a high odor grade even after 8 weeks. This study provides a theoretical basis and practical reference for the green extraction of Phoenix Dancong tea essential oil and its application in smart aromatic textiles. Full article
(This article belongs to the Section Food Process Engineering)
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18 pages, 3499 KB  
Article
Minute Rebond: A Simple Method for Making Lab-Scale Rebonded Foam and Its Application as a Novel Soilless Growing Media
by Michael S. Harris, Harry Charles Wright, Tom Lilly, Nathan Seithel, Chris Hayes, Julie Walker, Jacob Nickles, Duncan Drummond Cameron and Anthony John Ryan
Polymers 2025, 17(20), 2770; https://doi.org/10.3390/polym17202770 - 16 Oct 2025
Viewed by 1025
Abstract
Polyurethane foams (PUFs) utilised in the comfort industry generate substantial trim waste volumes requiring end-of-life management. Rebonding, one form of mechanical recycling, is a technique involving the mechanical breakdown and subsequent adhesion of PUF using polyurethane prepolymers yielding a recycled material. However, the [...] Read more.
Polyurethane foams (PUFs) utilised in the comfort industry generate substantial trim waste volumes requiring end-of-life management. Rebonding, one form of mechanical recycling, is a technique involving the mechanical breakdown and subsequent adhesion of PUF using polyurethane prepolymers yielding a recycled material. However, the limited investigation into the properties of rebond PUF constrains its potential for novel alternative uses, such as soilless plant-growing media. A laboratory-scale rebond production method has been developed, and a series of rebond PUFs produced to evaluate the influence of crumb size, density, prepolymer chemistry, and prepolymer loading on the properties of the rebond PUFs and their suitability as growing media. The results indicated that higher quality rebonds were obtained with larger crumb sizes (mixed or >7 mm), moderate amounts of prepolymer (4.5 to 7.5% by mass), and higher densities. Increasing density directly influenced plant growth-related properties, including reducing airflow, increasing water uptake through wicking, and increasing water retention through drainage alongside larger crumb sizes [>7 mm]. To demonstrate the method’s utility for rapid screening, a plant growth trial was conducted using density as the key variable. Eruca sativa plants grown in low-density rebonds exhibited comparable growth (leaf length, leaf width, and shoot fresh weight) to mineral wool, whereas medium- and high-density rebonds showed reduced growth. This study validates a lab-scale technique that enables the rapid optimisation of rebond PUFs for novel applications like soilless growing media. Full article
(This article belongs to the Section Circular and Green Sustainable Polymer Science)
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Article
A Highly Active Keratinase from Bacillus sp. FJ-3-16 for Sustainable Feather Waste Valorization and Eco-Friendly Industrial Applications
by Fei Bian, Hailun He, Gao Chen, Shousong Yue, Yaoxia Zhu, Xiaowei Zhang and Bin-Bin Xie
Biomolecules 2025, 15(10), 1389; https://doi.org/10.3390/biom15101389 - 29 Sep 2025
Cited by 2 | Viewed by 2219
Abstract
Keratinous biomass, such as feathers, wool, and hair, poses environmental challenges due to its insoluble and recalcitrant nature. In this study, we identified, purified and comprehensively characterized a previously uncharacterized extracellular alkaline keratinase, KerFJ, secreted by Bacillus sp. FJ-3-16, with broad industrial application [...] Read more.
Keratinous biomass, such as feathers, wool, and hair, poses environmental challenges due to its insoluble and recalcitrant nature. In this study, we identified, purified and comprehensively characterized a previously uncharacterized extracellular alkaline keratinase, KerFJ, secreted by Bacillus sp. FJ-3-16, with broad industrial application potential. KerFJ was produced at high yield (1800 U/mL) in an optimized cost-effective medium and purified to homogeneity using ion-exchange chromatography. The enzyme exhibited optimal activity at pH 9.5 and 55 °C, with remarkable alkaline and thermal stability, and high tolerance to surfactants, oxidants, and metal ions. Sequence analysis revealed that KerFJ is a member of the serine peptidase S8 family, with a molecular weight of ~27.5 kDa. It efficiently degraded native keratin substrates, achieving 70.3 ± 2.1% feather, 39.7 ± 1.8% wool, and 15.4 ± 1.2% hair degradation, and the resulting feather hydrolysates exhibited strong antioxidant activities. KerFJ also demonstrated excellent compatibility with commercial detergents and enabled effective stain removal from fabrics without damage. Moreover, both laboratory- and pilot-scale trials showed that KerFJ facilitated non-destructive dehairing of sheep, donkey, and pig skins while preserving collagen integrity. These results highlight KerFJ as a robust and multifunctional biocatalyst suitable for keratin waste valorization, eco-friendly leather processing, and detergent formulations. Full article
(This article belongs to the Special Issue Industrial Microorganisms and Enzyme Technologies)
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