Search Results (1,675)

Using plant byproducts as bioactive sources for in ovo injection (IOI) can enhance embryo development. This study evaluated the effects of air cell IOI of sweet orange peel (SP), pomegranate peel (PP), and olive leaf (OL) aqueous extracts on embryonic days 10 and 18, assessing chicken hatching and post-hatch performance up to 42 days of age. Nine hundred eggs were assigned to 10 treatments. Each treatment had three replicates (n = 30 eggs/replicate) with a 5 × 2 factorial design (uninjected negative control, injection with distilled water as positive control, or injection with 1% SP, PP, or OL on day 10 or 18 of embryogenesis). Compared to the negative control, the results revealed that in ovo-injected substances (IOSs) did not alter hatchability but significantly decreased pipped-chick percentage, the heterophil-to-lymphocyte ratio, malondialdehyde, cholesterol, triglycerides, and glucose levels. However, IOSs were found to increase superoxide dismutase (SOD) levels, liveability, and final body weight. Specifically, SP maximised hatch weight, gut length, and thymus weight, whilst decreasing eggshell conductance and uric acid. SP and OL reduced liver enzyme activities, whereas PP lowered creatinine. Compared to day 10, IOI on day 18 improved hatchability, packed cell volume, SOD activity, liveability, and organ development. In conclusion, IOI with SP or OL, particularly on day 18 of incubation, is recommended to improve antioxidant status, biochemical indices, and cumulative body weight. Full article

Scientific knowledge-based fishery management is essential to ensure the sustainability of marine resources, particularly in regions where fisheries are data-limited. This study assessed the stock status of six shallow-water demersal fish species (Bagre marinus, Cathorops mapale, Diapterus rhombeus, Eucinostomus argenteus, Haemulopsis corvinaeformis, and Lutjanus synagris) in the Colombian Caribbean Sea using three complementary length-based models: length-based indicators (LBIs), length-based spawning potential ratio (LBSPR), and the Length-Based Bayesian Biomass estimator (LBB). The integrated results demonstrated that five species (C. mapale, D. rhombeus, E. argenteus, H. corvinaeformis, and L. synagris) are currently overexploited (F/M > 1 and B/B_MSY < 1), while B. marinus is experiencing overfishing (F/M > 1 and B/B_MSY > 1), with a high risk of surpassing its maximum sustainable yield. These outcomes confirm that demersal fish populations in the Colombian Caribbean are being exploited beyond sustainable biological limits. With the aim of promoting stock recovery and long-term sustainability, this study recommends the implementation of recently evaluated management measures focused on (i) the implementation and enforcement of Bycatch Reduction Devices (BRDs); (ii) the regulation and monitoring of trawl net mesh sizes to improve selectivity patterns; (iii) the establishment of spatial and temporal closures in critical spawning areas for demersal fish species; and (iv) the strengthening of fishery monitoring and data collection systems. The findings provide critical baseline information and a methodological framework to support evidence-based fishery management and conservation strategies in tropical multispecies fisheries under data-limited conditions. Full article

Cetacean mortality serves as a critical indicator of marine ecosystem health, reflecting the cumulative impacts of climate-driven environmental shifts and anthropogenic pressures. However, long-term national-scale assessments remain limited in Thailand. This study aimed to assess population status, analyse spatiotemporal mortality patterns, and evaluate the influence of environmental drivers and anthropogenic stressors in Thai waters over the past two decades. Secondary data from multiple sources were analysed using generalised linear models with a negative binomial distribution. A total of 29 cetacean species were recorded, with an estimated population of approximately 3000 individuals. Mortality was documented in 24 species and showed an increasing trend over time. Coastal species, particularly Irrawaddy dolphins and finless porpoises, accounted for the majority of deaths (56%). Mortality patterns varied significantly by region (p < 0.05) but not by season, with the highest levels observed in the Upper Gulf of Thailand. Environmental factors were significantly associated (p < 0.05) with mortality, including wind speed in the Andaman Sea and extreme conditions (drought and heavy rainfall) in the Upper Gulf. In the Lower Gulf of Thailand, mortality was significantly associated with a combination of environmental (sea surface temperature and wind speed) and anthropogenic factors (fishery production). Overall, environmental variability appeared to exert a stronger influence than anthropogenic stressors. These findings highlight the requirement for targeted monitoring in high-risk regions and periods, alongside improved investigation of mortality causes to support effective conservation strategies. Full article

Cold stress is a major environmental constraint limiting the growth, physiological performance, and productivity of African marigold (Tagetes erecta L.) under open-field conditions. This study evaluated the comparative effectiveness of salicylic acid (SA), silicon (Si), and methyl jasmonate (MeJA) in alleviating cold-induced damage and enhancing stress tolerance. Field experiments were conducted under naturally occurring cold stress using foliar applications of SA (0, 0.1, 0.5, and 1 mM), Si (0, 1, 5, and 10 mM), and MeJA (0, 10, and 50 µM) in a complete randomized block design with three replications. Moderate concentrations of all three regulators significantly (p < 0.05) improved plant growth and physiological stability relative to untreated controls. Salicylic acid at 0.5 mM produced the most consistent protective response, increasing biomass accumulation, chlorophyll content, and relative water content while reducing membrane damage, as indicated by a 42.3% decrease in leaf electrolyte leakage at 2 °C. Silicon at 10 mM enhanced membrane integrity, plant water status, and vegetative growth under low-temperature conditions, while methyl jasmonate at 10 µM mitigated cold-induced membrane damage and improved physiological tolerance, whereas higher concentrations (50 µM) were less effective. At their optimal doses, SA, Si, and MeJA increased plant dry mass by 39.7%, 30.1%, and 38.5%, respectively. Correlation analysis confirmed these results, revealing strong positive relationships among growth, chlorophyll, and relative water content. Conversely, electrolyte leakage was negatively correlated with biomass and water status, identifying membrane stability as a key determinant of cold tolerance. Overall, 0.5 mM SA, 5–10 mM Si, and 10 μM MeJA improved growth and key physiological responses in African marigold under cold stress under field conditions. Future studies should integrate mechanistic and economic analyses to refine growth-regulator-based cold-stress management strategies. Full article

This non-randomized, open-labelled, controlled pilot trial investigated the impact of stunting on oral health and the oral microbiome, and evaluated the effect of 14-day probiotic or essential oil mouthwash interventions in children aged 8–12 years. Thirty-six participants (18 stunted, 18 non-stunted) were randomized into three parallel arms: probiotic lozenges (Limosilactobacillus reuteri DSM 17938 + ATCC PTA 5289), essential oil mouthwash, or water control. D-25OH level was assessed with ELISA, OHI-S, and PBI were examined, and oral microbiome was analyzed using 16S metagenomic sequencing. Stunted children demonstrated significantly higher gingival inflammation (PBI, F = 10.57, p = 0.002), reduced microbial alpha diversity, reductions in commensal Streptococcus spp., and increases in pathobionts, including Parvimonas micra, Fusobacterium nucleatum, and Tannerella forsythia. Beta-diversity analysis revealed distinct microbial communities (p = 0.001), with network analysis identifying these anaerobes as keystone hubs in stunted individuals. Salivary vitamin D and oral hygiene indices (OHI-S) also differed by stunting status. Fourteen-day interventions produced only modest, non-significant improvements in clinical indices and failed to induce significant shifts in microbial diversity or composition. These findings indicate that nutritional stunting is independently associated with oral dysbiosis and gingival inflammation. Short-term antiseptic interventions appear insufficient to reverse established microbial shifts, highlighting the need for sustained, integrated nutritional—oral health strategies. Full article

The sustainable management of water supply reservoirs requires analysis of spatiotemporal variations in nutrient levels, phytoplankton composition, and trophic status. The Katse Dam (KD) is a strategic raw water supply source that generates hydropower and sustains aquaculture. However, it is exposed to nutrient enrichment from mining and aquaculture, whose impact on its trophic status necessitates monitoring. This study applies the organic pollution index (OPI), the modified pollution index (MPI), and Carlson’s trophic state index (CTSI) to assess the trophic status of KD. The results from the first decade (FD) (2003–2013), when the intensity of mining and aquaculture activities was minimal, were compared with the results from the second decade (SD) (2014–2024) when there was higher activity. The MPI revealed that KD transitioned from a contaminated status during the FD to a greatly contaminated status during the SD. KD shifted from mesotrophic to eutrophic in the transitional zone and from eutrophic to hypereutrophic in the lacustrine zone. The cyanobacteria Radiocystis sp. replaced Asterionella sp. and became the most abundant algae in the SD, followed by the diatom Flagilaria sp. Principal component analysis (PCA) indicated stronger correlations between NH₄, PO₄, NO₃, and NO₂, while canonical correspondence analysis (CCA) indicated a strong correlation between PO₄ and Fragilaria sp. in the SD. The OPI classified KD water quality as excellent, with the exception of the lacustrine zone, where the water quality was polluted in 2016 and 2021. The data analysis revealed how long-term variations in KD water chemistry and phytoplankton influenced trophic status. This study thus provides water managers with a template for assessing water quality to secure the strategic value of the KD. Full article

Aim: This study assessed the effects of a six-week educational intervention using an adapted “Snakes and Ladders” board game on hydration knowledge, behavior, and status among Tunisian elementary school children during physical education (PE) lessons. Method: A randomized controlled trial involving 207 children was conducted, with participants assigned to either the educational group (EG, n = 99) or the control group (CG, n = 108). The EG participated in six weekly 30-min board game sessions, while the CG continued regular activities. Knowledge of hydration was assessed using a validated questionnaire. Hydration status was monitored indirectly by the percentage change in body mass from pre- to post-PE session. Perceived thirst was evaluated using a 9-point scale, and hydration behavior was evaluated based on water consumption during PE lessons. Results: Following the intervention, the EG demonstrated a significant improvement in overall hydration knowledge (ΔMean = +0.30 ± 0.11 vs. −0.05 ± 0.08 in CG; p < 0.001). Water intake during PE sessions increased progressively from week 2 (83.6 ± 127.2 mL) to week 6 (311.2 ± 204 mL) in the EG, whereas it remained unchanged in the CG (p < 0.001). Body mass loss after PE sessions decreased significantly in the EG (−0.03 kg) compared with the CG (−0.16 kg; p < 0.001), and perceived thirst before and after PE sessions was markedly lower (Cohen’s d = 0.75–1.32). Conclusions. The six-week board-game intervention appeared effective in increasing hydration knowledge, promoting healthier drinking behaviors during PE, and improving indirect indicators of hydration status. These findings provide preliminary evidence for the feasibility and educational value of a gamified, low-cost approach to hydration promotion in schools. Further research should examine long-term retention, include objective hydration biomarkers, and evaluate applicability across diverse school settings and environmental conditions. Full article

Refractory status epilepticus refers to persistent and recurrent seizures unresponsive to medication, often leading to neuronal injury and neurobehavioral deficits. Studies have demonstrated that intraperitoneal bolus administration of pyruvate attenuates neuronal damage in rodent models of chemically induced status epilepticus (SE), while the precise neuroprotective mechanism remains to be further explored. This study investigated the neuroprotective effects of long-term supplementation with exogenous pyruvate against SE. When male C57BL/6 mice received 3% sodium pyruvate (SP) in the drinking water ad libitum for 20 weeks, they exhibited elevated levels of essential neurochemicals and energy metabolites in the brain compared to the control mice that received the equimolar saline solution. Following the intraperitoneal administration of kainate (KA) to induce severe SE, the SP-fed mice showed enhanced resistance to seizure activity, reduced neuronal injury, and improved neurobehavioral performance compared to the saline-fed mice. Regarding the molecular mechanisms underlying their neuroprotective properties, the levels of pyruvate metabolism-mediating proteins, neuronal and synaptic proteins, and neuroprotective proteins remained upregulated in the brains of the SP-fed mice following KA-induced SE. Conversely, the levels of pro-apoptotic and oxidative stress markers were suppressed. Collectively, this study indicates that long-term pyruvate supplementation may sustainably augment neurochemical and energy metabolism in the normal brain, thereby eliciting intrinsic neuroprotective properties. These effects contribute to preventing or ameliorating seizure activity, neuronal damage, and neurobehavioral deficits in mice following KA-induced SE, suggesting its prophylactic or therapeutic potential against epileptic seizures and SE through metabolic preconditioning. Full article

The increasing frequency and intensity of hotter droughts are driving widespread forest dieback, yet the role of tree nutritional status in drought-induced growth dieback remains poorly understood. We investigated how nutrient composition across tissues (leaves, wood) relates to water use patterns and growth resilience in rear-edge populations of Scots pine (Pinus sylvestris L.) in Northeastern Spain. Using a multi-proxy approach, we combined analyses of foliar and sapwood nutrient concentrations, stable isotopes (δ¹³C, δ¹⁸O), and dendrochronological indicators across contrasting tree vigor classes. Defoliated trees exhibited pronounced shifts in elemental composition, including depletion of foliar K and increased concentrations of Ca, S, and Fe, alongside higher intrinsic water use efficiency and reduced growth resistance to drought. In contrast, the sapwood elemental composition was less responsive to defoliation but showed stronger associations with isotopic signals and drought resilience, suggesting its integrative role in tree functioning. Coordination of nutrient concentrations between tissues was limited, suggesting organ-specific regulation of nutrient allocation under drought stress. Our results reveal that nutrient imbalances are linked to water–carbon dynamics and drought responses and emphasize the importance of considering multi-organ nutrient dynamics to improve our understanding of long-term nutritional imbalances during drought-induced forest dieback. Full article

To scientifically evaluate the health of river aquatic ecosystems in the Qianxinan Buyi and Miao Autonomous Prefecture, southwestern Guizhou, systematic surveys of benthic macroinvertebrate and periphytic algal communities were conducted in representative rivers during October 2024 (autumn) and April 2025 (spring), coupled with concurrent water quality monitoring. Reference sites were selected based on water quality indicators and habitat conditions. Core parameters were identified through correlation analysis, discriminatory ability analysis, and distribution range analysis to construct a Benthic Index of Biotic Integrity (B-IBI) and a Periphytic Algae Index of Biotic Integrity (P-IBI) suitable for the region. These indices were then applied to assess the ecological health of the rivers. Additionally, stepwise regression analysis was employed to investigate the key environmental drivers influencing the two biotic integrity indices. The results indicated that: (1) In terms of species composition, the benthic macroinvertebrate community structure was relatively simple, dominated by arthropods, particularly chironomid larvae. Bacillariophyta and Cyanophyta consistently dominated the periphytic algae community. (2) Assessments using both B-IBI and P-IBI showed that the overall river health in spring was slightly better than in autumn. However, more than half of the sampling sites were rated as “fair” or worse in both seasons. The reference sites (S2, S10) consistently exhibited “excellent” or “good” health, while the impaired sites showed significant spatial heterogeneity. Discrepancies between B-IBI and P-IBI ratings at some sites revealed differential responses of the two biological communities to environmental stressors. (3) Stepwise regression analysis unveiled a seasonal shift in key environmental drivers. The primary factor affecting the B-IBI in autumn was biochemical oxygen demand (BOD₅), which shifted to total phosphorus (TP) and ammonia nitrogen (NH₄⁺-N) in spring. For the P-IBI, the main factor changed from dissolved oxygen (DO) in autumn to chemical oxygen demand (COD) in spring. These findings confirm the applicability of the B-IBI and P-IBI systems in this region, and indicate that multi-assemblage integrated assessments can contribute to understanding the health status of river ecosystems in the Qianxinan Prefecture. This study could serve as a scientific reference for the protection, management, and restoration of local river ecosystems. Full article

This study aimed to investigate the regulatory effect and cryoprotective mechanism of melatonin (MT) on the physiological functions of Lactobacillus plantarum FQR during freezing and freeze-drying. Results indicated that the addition of 5 mg/mL MT as a cryoprotectant maximized the freeze-drying survival rate to 32.04 ± 2.14%. MT effectively alleviated low-temperature and freeze-drying stress by reducing extracellular alkaline phosphatase activity, enhancing intracellular lactate dehydrogenase activity, and decreasing extracellular β-galactosidase activity without significant differences. Higher survival rates in defining medium further suggested that MT reduced damage to cell wall and membrane structures during lyophilisation, decreased membrane permeability, and preserved cellular physiological functions. In addition, MT supported cellular energy metabolism and protein synthesis, enhanced transmembrane potential to facilitate ATP transport, and helped maintain intracellular and extracellular pH balance. The prepared freeze-drying protectant containing 69.80 mg/mL exopolysaccharides (EPS) and 4.25 mg/mL MT showed better protective effects than the control group. MT also increased bound water content, lowered the freezing point of the solution, and inhibited ice crystal formation. Transcriptomic analysis revealed that amino acid biosynthesis, amino acid metabolism, and ABC transport systems were the primary pathways affected by MT treatment. These findings demonstrate that MT improves freeze-drying tolerance by maintaining membrane integrity, regulating cellular metabolism, and enhancing oxidative stress resistance. Given its natural biosynthetic origin, generally recognized as safe (GRAS) status, and absence of residual solvents or allergenic proteins, MT can be safely considered for incorporation into food and nutraceutical products. This study underscores the practical relevance of MT as a functional component in compound cryoprotectants, providing a feasible strategy to enhance the viability, stability, and industrial applicability of Lactobacillus plantarum during freeze-drying and storage. Full article

Toxic metals from industrialization and urbanization pose major human health risks, and mixture-based exposure requires broader toxicity assessment. This study investigated the effects of a mixture of arsenic, lead, mercury, cadmium, chromium (VI), and nickel on pancreatic function in rats (45 male/45 female; n = 5 per group), focusing on sex- and duration-specific differences after 28 and 90 days of exposure. The metals were administered as a single mixture dissolved in deionised water via oral gavage. Evaluated parameters included pancreatic metal levels, histopathology, serum glucose, amylase, malate dehydrogenase 1 (MDH-1) activity, redox status, and bioelements. Dose levels were based on human exposure data to reflect realistic scenarios. Metals accumulated in pancreatic tissue, causing dose- and time-dependent histopathological changes, including acinar cell vacuolization, vascular congestion, and Langerhans islet alterations. Males showed more pronounced vascular and islet changes, while females had greater acinar alterations. In males, higher doses decreased glucose and amylase and increased MDH-1 activity, while females showed more variable responses. Males demonstrated adaptive responses to oxidative stress over time, while females experienced more persistent stress. These findings reveal sex-, dose-, and duration-dependent effects of toxic metal(oid) mixtures on pancreatic function, indicating that individually safe doses may be harmful when combined. Full article

Drought severely limits maize yields. Water–saving irrigation methods like partial root–zone drying (PRD) can improve water use efficiency (WUE) but often result in variable yield responses among genotypes. We hypothesized that differences in root hydrotropism might contribute to some of this variability. Seven maize varieties were evaluated for hydrotropic response in a controlled moisture–gradient assay and then grown for five weeks under fixed PRD versus full irrigation in a greenhouse. The different maize varieties exhibited distinct hydrotropic behaviors: roots of V6 and V7 bent toward water much faster and more strongly, while V2 responded slowly with minimal curvature. Under PRD, genotypes also differed in root distribution and shoot performance. However, hydrotropism alone did not guarantee good shoot maintenance. One strongly hydrotropic genotype (V7) still suffered a large biomass reduction under PRD. Overall, genotypes that maintained better shoot water status, along with larger stem diameter and higher shoot water content, achieved the highest WUE under PRD. These results indicate that root hydrotropism varies widely in maize varieties. This variation was associated with shoot traits and WUE under PRD, suggesting that the benefit of hydrotropism for drought adaptation may depend on complementary shoot characteristics. Breeding for drought–resilient maize may therefore require combining strong root hydrotropism with the ability to maintain shoot function under water deficit. Full article

The progressive degradation of natural habitats, driven by anthropogenic pressures and climate change, constitutes one of the most serious threats to biodiversity. Peatland ecosystems, along with the valuable plant species associated with them, are particularly vulnerable to these processes. Salix lapponum, a glacial relict species, is undergoing a drastic decline in both its range and population size across Poland and Europe. This emphasizes the need for the implementation of conservation measures, including species translocation, as well as the development of effective methods for monitoring plant condition following introduction. The aim of this study was to evaluate the usefulness of selected physiological indicators for the rapid and reliable assessment of plant condition in active conservation efforts. The experimental material consisted of S. lapponum plantlets derived from tissue culture, which were introduced into five experimental sites in eastern Poland, differing in habitat conditions. Over two growing seasons, chlorophyll fluorescence parameters (F₀, F_m, F_v/F_m), the content of photosynthetic pigments and anthocyanins, relative water content, guaiacol peroxidase activity, and the presence of reactive oxygen species were analyzed. The results revealed clear seasonal variability in most of the studied physiological parameters, as well as their differentiation across habitat conditions. The highest sensitivity to environmental changes was observed for indicators related to photosynthetic performance (F_v/F_m), tissue hydration status (RWC), and enzymatic activity. Declines in photosystem II efficiency at the beginning of the growing season, reflected in F_v/F_m values decreasing to 0.47–0.49 indicate transient stress conditions in plants. Simultaneously, variation in relative water content (52–90%) and peroxidase activity reflects differences in water availability and the intensity of environmental stress across habitats. The findings confirm that selected physiological indicators can serve as effective tools for the early monitoring of plant condition and for assessing the success of S. lapponum translocation. Full article

Hydrogen energy is an important carrier for achieving China’s “dual carbon” goals, and one of the sources of green hydrogen is to develop better water electrolysis catalysts. This paper reviews the current research status of water electrolysis hydrogen production catalysts, analyzes the role and significance of advanced hydrogen energy catalysts in achieving the “dual carbon” goals, and conducts an in-depth analysis of the difficulties in moving from the laboratory to large-scale application, namely, how to bridge the “four gaps”, including catalyst performance evaluation, long-term application of catalysts, macro-scale preparation, and device integration. It also proposes overall improvement ideas and measures. In this paper, effective improvement methods are proposed for these “four gaps”, which can improve the relevant indicators and service life of water electrolysis hydrogen production catalysts, further promote the large-scale production and industrial application of green hydrogen, and provide a strong guarantee for solving China’s “dual carbon” problems. Full article