Search Results (31)

Background: Inflammation is a common reason for dogs to present to veterinary clinics. Early diagnosis of systemic inflammation is important. Acute phase proteins, like C-reactive protein, are useful but not specific to infection. In human medicine, the intensive care infection score (ICIS) offers a faster, cost-effective alternative using advanced hematological parameters. While ICIS is not available for veterinary use, some components (e.g., neutrophil side fluorescent light) can be measured using analyzers like the Sysmex XN-1000V. Objectives: This study aimed to establish a control group of healthy dogs for the novel parameters neutrophil side fluorescent light (NE-SFL), neutrophil side scattered light (NE-SSC), and neutrophil forward scattered light (NE-FSC) and assess their utility in detecting inflammation in diseases such as sepsis, pyometra, steroid-responsive meningitis-arteritis (SRMA), and idiopathic epilepsy. Methods & Results: Value ranges were calculated based on 21 healthy dogs. Compared to controls, NE-SFL levels were significantly elevated in sepsis, pyometra, and SRMA, while NE-SSC was only elevated in sepsis and pyometra and NE-FSC only in sepsis. No increases were observed in idiopathic epilepsy. Manual gating of the white blood cell differential scattergram was necessary in samples showing high neutrophil toxicity and the presence of bands. Conclusion: NE-SFL and NE-SSC, obtainable from routine complete blood count, may serve as novel, accessible markers for inflammation in dogs. Further research is needed to validate their broader diagnostic use. Full article

Backyard poultry systems (BPS) are the most widespread form of animal production worldwide, contributing to household economies and improving food availability. However, limited biosecurity measures and close human–animal interactions raise concerns regarding zoonotic disease transmission. In recent years, consumer-driven motivations have given rise to non-traditional BPS with differential attributes (BPS-DA), yet there is limited knowledge about their food production practices. This study aimed to characterize and compare practices across 25 BPS and 25 BPS-DA in the Metropolitan Region using surveys, interviews, and direct observations of egg collections and poultry slaughters. Eggs were the main animal product in both systems, with women primarily responsible for care. Poultry slaughter was reported exclusively in BPS (60%), generally performed under inadequate hygienic conditions and without veterinary oversight. These practices, (poultry slaughter, food production and handling), may considerably increase the risk of human exposure to zoonotic pathogens, such as avian influenza viruses. In contrast, BPS-DA prioritized birds as companion animals (60%), free-range rearing (68%), and hobby-based production (80%). While both systems showed limited biosecurity, significant differences were found in the use of dedicated footwear (p = 0.01; V = 0.35), egg collection sites (p = 0.04; V = 0.29), and refrigeration (p = 0.004; V = 0.41). Veterinary access was limited in both (32% in BPS; 44% in BPS-DA). These findings highlight critical gaps in health management and underscore the need for context-specific educational and regulatory strategies for safer backyard poultry production. Full article

Compounded medications (CMs) have become increasingly relevant in veterinary pharmacotherapy, particularly due to the limited availability of licensed veterinary medicinal products (VMPs). Despite the importance of CMs, data on their use all over the world, as well as in Serbian veterinary practice, remain limited. The aim of this study is to understand Serbian veterinarians’ attitudes towards the use of CMs and human medicinal products in small animal practice, with a focus on challenges and needs they experience related to this type of therapy. A questionnaire-based survey was conducted from January to May 2024 among licensed veterinarians, covering sociodemographics, antibiotic prescribing practices, and attitudes toward CMs. Most respondents (85.4%) reported difficulties in accessing appropriate medications, with 45.8% frequently prescribing human medications for reasons of availability and cost. While 58.3% were familiar with CMs, many emphasized their benefits in tailoring doses and formulations. A majority (65.6%) believed CMs could improve treatment outcomes, yet 87.5% expressed the need for further education. This study shows that Serbian veterinarians frequently prescribe human medications due to limited and costly veterinary medicinal products. While they see the benefits of CMs for personalized treatments, there is a clear need for improved training and clearer regulations to enhance treatment outcomes and ensure safer veterinary care. Full article

The recent spread of foreign animal diseases (FADs) such as foot-and-mouth disease and African swine fever emphasizes the need to conduct comprehensive surveillance to detect a potential disease introduction as soon as possible. The United States is currently free of many important FADs of swine, and many preparedness initiatives have raised awareness amongst the commercial, intensive swine industry. However, the awareness and engagement of small-scale swine farmers regarding disease surveillance and passive reporting is not well known. This scoping review was conducted to identify and characterize sources of evidence on the practices and attitudes of small-scale swine farmers and owners in the United States regarding pig health and disease management, surveillance, and veterinary care use, and secondarily to characterize information seeking and communication behaviors. Sources of evidence were found through keyword searches of online databases, citation matching, and Sustainable Agriculture Research and Education project reports. Eligibility criteria included being conducted on or with US small-scale (defined in this review as less than 1000 pigs) or non-intensive swine farms and containing information pertinent to the objectives of the review. Seventeen sources were included in the final review. Regular disease monitoring and surveillance practices were not commonly reported, and multiple sources reported little to no incidence of disease occurrence in small-scale swine farms. Reported veterinary use and access was variable, and multiple sources reported that the choice to use veterinary care was affected by its perceived cost, value, and accessibility. Future research and outreach should aim to discern key factors affecting farmer’s decisions to use a veterinarian, improve their awareness and prioritization of swine diseases, and develop small-scale appropriate disease surveillance protocols. Ultimately, this will help small-scale swine farmers to protect the health of their pigs and improve FAD surveillance in the US. Full article

Feline leukemia virus (FeLV) and Feline immunodeficiency virus (FIV) are globally prevalent retroviral pathogens that pose significant health risks to domestic cats. This study aimed to compare the diagnostic performance of two point-of-care—the immunochromatographic assay (ICA) and the RNase hybridization-assisted amplification (RHAM) test kit—against reverse transcription quantitative polymerase chain reaction (RT-qPCR), the current gold standard for FeLV and FIV detection. For FeLV detection, ICA demonstrated a sensitivity of 86.89%, specificity of 96.55%, accuracy of 90.00%, and precision of 98.15%, while for FIV detection, the assay showed a sensitivity of 75.86%, specificity of 88.52%, accuracy of 84.44%, and precision of 75.86%. In contrast, the RHAM test exhibited superior performance, with FeLV detection sensitivity of 93.44%, specificity of 98.28%, accuracy of 94.44%, and precision of 98.28%. For FIV detection, RHAM demonstrated a sensitivity of 75.86%, specificity of 100%, accuracy of 92.22%, and precision of 100%. Additionally, the RHAM assay significantly reduced detection time compared to RT-qPCR, enabling expedited clinical decision-making, alleviating laboratory workload, and lowering diagnostic costs. These benefits are particularly relevant in veterinary settings with limited access to PCR-based diagnostics, where the RHAM assay represents a rapid, reliable, and resource-efficient alternative for FeLV and FIV detection. Full article

(1) Background: Telemedicine is increasingly recognized as a potential way to help overcome barriers to accessing veterinary care. This study explored pet owners’ perspectives on telemedicine services in situation they viewed as veterinary emergencies, focusing on both their access-to-care challenges and their expectations of such services. (2) Methods: Semi-structured, one-on-one interviews were conducted with 18 pet owners in Ontario, Canada. All participants were unable to access in-person care, leading them to a veterinary telemedicine service. Interviews were supplemented by an online questionnaire to collect demographic information. Thematic analysis was conducted on all interview transcripts. (3) Results: Participants’ expectations of telemedicine were largely informed by their uncertainty about their pets’ health in emergency situations. Anxiety was common, leading them to seek telemedicine for reassurance. Participants expected advice and guidance, viewing the process as collaborative. While acknowledging the benefits of telemedicine in their situation, participants also acknowledged the inherent limitations of this type of service, articulating a general preference for in-person veterinary care. (4) Conclusions: When in-person care is unavailable, telemedicine can offer pet owners valuable guidance, clarity, and comfort, although many pet owners may continue to prefer face-to-face consultations for comprehensive care. Full article

(1) Background: Veterinary virtual care holds the potential to alleviate some barriers to accessing care, yet concerns within the profession exist. Understanding veterinarians’ perspectives and identifying the potential opportunities and challenges that virtual care poses for access to veterinary care are thus needed. (2) Methods: Semi-structured interviews were conducted virtually with 22 companion-animal veterinarians practicing across Canada and the United States. Interviews were accompanied by an electronic survey, with which a Net Promoter Score (NPS) was calculated for each participant. Using their NPS, participants were categorized as a “promoter” or “detractor”, with respect to their perspective on veterinary virtual care. A thematic analysis was conducted on verbatim transcripts of the interviews. (3) Results: A total of 11 detractors and 11 promoters were interviewed. Four subthemes were identified, including the following: (1) there are limitations to virtual care, (2) virtual care plays a role in access to care, (3) “virtual care is better than no care” and (4) virtual care offers specific value in supplementing in-person care. (4) Conclusion: When no other option for care delivery exists, virtual care was viewed as a way to increase access to veterinary care. Full article

Cancer is a leading cause of death among companion animals, with many cases diagnosed at advanced stages when clinical signs have appeared, and prognosis is poor. Emerging diagnostic technologies, including Artificial Intelligence (AI)-enhanced imaging, liquid biopsies, molecular diagnostics, and nematode-based screening, can improve early detection capabilities in veterinary medicine. These tools offer non-invasive or minimally invasive methods to facilitate earlier detection and treatment planning, addressing the limitations of traditional diagnostics, such as radiography and tissue biopsies. Recent advancements in comparative oncology, which leverage the biological similarities between human and companion animal cancers, underscore their translational value in improving outcomes across species. Technological advances in genomics, bioinformatics, and machine learning are driving a shift toward precision medicine, enabling earlier detection, personalized treatments, and monitoring of disease progression. Liquid biopsy testing detects circulating tumor DNA and tumor cells, providing actionable insights into tumor genetics without invasive procedures. Imaging systems enhance diagnostic precision, offering consistent and accurate tumor identification across veterinary practices, while portable innovations like Caenorhabditis elegans-based screening provide accessible options for underserved regions. As these technologies migrate from human medicine to veterinary applications, they are poised to redefine cancer care for companion animals. This review highlights key advancements in diagnostic technologies and their application in veterinary oncology, with a focus on enhancing early detection, accessibility, and precision in cancer care. By fostering the adoption of these innovations, veterinary oncology can achieve a new standard of care, improving outcomes for both animals and humans through the lens of comparative oncology. Full article

Most cats entering animal shelters in Australia are less than 12 months old and emanate from low socioeconomic areas. Many are unidentified and may be either owned or semi-owned cats (cats cared for by someone who does not consider themselves the owner). In Australia, approximately 33% of cats entering shelters and pounds are euthanized annually, adversely affecting the mental health of staff involved. Our study investigated cat ownership and caregiving behaviors of cats enrolled in the Australian Pet Welfare Foundation’s Community Cat Program, which included free sterilization, microchipping, and preventive healthcare. The suburbs targeted in Ipswich, Australia had a high per capita cat intake into animal shelters. We surveyed 1094 cat owners and semi-owners. Most cats were under 12 months old (79%), primarily domestic short-haired (69%), and acquired through informal channels such as friends, family, or as strays. Veterinary care was limited, with only 15% of cats having visited a veterinarian and 28% being vaccinated. Prior to hearing about the free sterilization program, 88.5% of participants identified as owners, while 11.5% were semi-owners. Before completing the survey, these semi-owners transitioned from an informal caregiving relationship to becoming owner of the cat/s. Of the semi-owners, 93% indicated that their transition to ownership was driven by feelings of responsibility, emotional attachment, and access to free sterilization services. These findings suggest that targeted interventions, such as accessible veterinary care and sterilization programs, are crucial in encouraging semi-owners to take full ownership of cats, improving cat welfare, and reducing unplanned breeding. Supporting this transition helps curb overpopulation and fosters improved caregiving, benefiting both cats and communities. Full article

Access to veterinary care for animal owners is an important part of keeping animals healthy and keeping pets and people together whenever that is appropriate. Insufficient financial and other resources to allocate to veterinary care are major barriers for pet owners to receiving preventative, sick, and emergency services. The veterinary community has begun to incorporate offering a range of diagnostic and treatment options more intentionally in response to clients’ inability to pay and to a lesser extent to mitigate other barriers to care. Many veterinarians are nonetheless oriented toward providing specialized and more sophisticated care based on their training. This study sought to identify the self-reported behaviors, knowledge, attitudes, and beliefs of veterinarians about offering a spectrum of care options (SoC) to clients. The finding that many reported offering SoC is encouraging. However, veterinarians who report comfort and confidence in a variety of aspects of clinical care were most likely to offer SoC. Practitioners in the field for 20 or more years were less likely to offer SoC to clients with financial limitations. Rural veterinarians were more likely to offer SoC to any client compared to urban veterinarians. These results provide a point of reference and potential focus for veterinarians who are not currently offering SoC as well as an exploration of veterinarians’ reported knowledge, behaviors, attitudes, perceptions, and concerns about SoC. Full article

The use, misuse, and overuse of antimicrobials is one of the main public health threats of the 21st century. We investigated the risk factor of the presence of extended-spectrum, cephalosporin-resistant Enterobacterales in feces of non-domestic and domestic birds and other domestic animals in Piauí State, northeast Brazil. We collected a total of 387 cloacal and rectal swab samples of free-living birds, domestic birds, and domestic mammals in five municipalities: Amarante, Água Branca, Lagoa Alegre, Parnaíba, and Teresina. A total of 59/387 (15.2%) of these samples harbored extended spectrum beta-lactamase (ESBL)-producing Enterobacterales. Using the MALDI-TOF technique, we identified fifty-seven samples as Escherichia coli and two samples as Klebsiella pneumoniae. Teresina and Parnaíba had the highest prevalence of animals with resistant bacteria (32.1% and 27.1%, respectively) and highest exposure risk factor (OR of 16.06 and 8.58, respectively, and p < 0.001 for all). Multidrug-resistant, ESBL-producing Enterobacterales were observed in 72.8% of the samples (43/59). For the free-living birds, the positive samples belonged to a great kiskadee (Pitangus sulphuratus) and a semipalmated sandpiper (Calidris pusilla) in migratory and resident species, respectively. For domestic animals, the swine samples showed the highest prevalence of antimicrobial resistance. The lack of access to veterinary care and information regarding antimicrobial therapy, along with the easy access to antimicrobials without medical prescription, favors the inadequate use of antimicrobials in Piauí. Full article

The global spread of African swine fever (ASF) in recent decades has led to the need for technological advances in sampling and diagnostic techniques. The impetus for these has been the need to enable sampling by lay persons and to obtain at least a preliminary diagnosis in the field for early control measures to be put in place before final laboratory confirmation. In rural Africa, rapid diagnosis is hampered by challenges that include lack of infrastructure as well as human and financial resources. Lack of animal health personnel, access to affordable means to transport field samples to a laboratory, and lack of laboratories with the capacity to make the diagnosis result in severe under-reporting of ASF, especially in endemic areas. This review summarizes the challenges identified in gap analyses relevant to low- and middle-income countries, with a focus on Africa, and explore the opportunities provided by recent research to improve field diagnosis and quality of diagnostic samples used. Sampling techniques include invasive sampling techniques requiring trained personnel and non-invasive sampling requiring minimal training, sampling of decomposed carcass material, and preservation of samples in situations where cold chain maintenance cannot be guaranteed. Availability and efficacy of point-of-care (POC) tests for ASF has improved considerably in recent years and their application, as well as advantages and limitations, are discussed. The adequacy of existing laboratory diagnostic capacity is evaluated and opportunities for networking amongst reference and other laboratories offering diagnostic services are discussed. Maintaining laboratory diagnostic efficiency in the absence of samples during periods of quiescence is another issue that requires attention, and the role of improved laboratory networking is emphasized. Early diagnosis of ASF is key to managing the disease spread. Therefore, the establishment of the Africa Chapter of the Global African Swine Fever Research Alliance (GARA) increases opportunities for collaboration and networking among the veterinary diagnostic laboratories in the region. Full article

Feline leukemia virus (FeLV) remains a serious concern in some countries despite advances in diagnostics and vaccines. FeLV-infected cats often have reduced lifespans due to FeLV-associated diseases. The infection is transmitted through social interactions. While Northern European countries have reported a decrease in FeLV among pet cats, Switzerland’s rates remain stagnant at 2.7% (2016/17: 95% CI 1.4–5.2%). Research on FeLV in Swiss stray cats has been lacking, even though these animals could serve as a virus reservoir. Sampling stray cats that do not receive regular veterinary care can be challenging. Collaboration with the Swiss Network for Animal Protection (NetAP) allowed for the prospective collection of saliva samples from 1711 stray cats during a trap–neuter–return program from 2019 to 2023. These samples were tested for FeLV RNA using RT-qPCR as a measure for antigenemia. Viral RNA was detected in 4.0% (95% CI 3.1–5.0%) of the samples, with 7.7% (95% CI 4.9–11.3%) in sick cats and 3.3% (95% CI 2.4–4.4%) in healthy ones. We identified three geographically independent hotspots with alarmingly high FeLV infection rates in stray cats (up to 70%). Overall, including the previous data of privately owned cats, FeLV-positive cats were scattered throughout Switzerland in 24/26 cantons. Our findings underscore welfare concerns for FeLV infections among stray cats lacking veterinary attention, highlighting the potential risk of infection to other free-roaming cats, including those privately owned. This emphasizes the critical significance of vaccinating all cats with outdoor access against FeLV and developing programs to protect cats from FeLV infections. Full article

Lower urinary tract dysfunction, such as incontinence or urinary retention, is one of the leading consequences of neurological diseases. This significantly impacts the quality of life for those affected, with implications extending not only to humans but also to clinical veterinary care. Having motor and sensory fibers, the pudendal nerve is an optimal candidate for neuromodulation therapies using bidirectional intraneural prostheses, paving the way towards the restoration of a more physiological urination cycle: bladder state can be detected from recorded neural signals, then an electrical current can be injected to the nerve based on the real-time need of the bladder. To develop such prostheses and investigate this novel approach, animal studies are still required since the morphology of the target nerve is fundamental to optimizing the prosthesis design. This study aims to describe the porcine pudendal nerve as a model for neuromodulation studies aiming at restoring lower urinary tract dysfunction. Five male farm pigs were involved in the study. First, a surgical procedure to access the porcine pudendal nerve without muscle resection was developed. Then, an intraneural interface was implanted to confirm the presence of fibers innervating the external urethral sphincter by measuring its electromyographic activity. Finally, the morphophysiology of the porcine pudendal nerve at the level of surgical exposure was described by using histological and immunohistochemical characterization. This analysis confirmed the fasciculate nature of the nerve and the presence of mixed fibers with a spatial and functional organization. These achievements pave the way for further pudendal neuromodulation studies by using a clinically relevant animal model with the potential for translating the findings into clinical applications. Full article

This research aimed to explore cat and dog owners’ attitudes and opinions towards various pet care practices in Aotearoa New Zealand (NZ), and determine what factors were associated with them. An online survey composed of both closed and open-ended questions was administered from January to March 2019. A total of 2358 respondents answered the questions on pet care practices. Of these, 37.5 percent (n = 885) were both dog and cat owners, 28.0 percent (n = 652) were cat owners, and 26.0 percent (n = 609) were dog owners, while 9% (n = 212) of respondents did not own a cat or dog at the time of taking the survey. The study revealed that most respondents (>90%) acknowledge the importance of providing adequate housing, regular worming and flea treatments, microchipping, and vaccinations for their pets. Notably, demographic factors such as gender, ethnicity, age range, having children, having a rural upbringing, and place of residence influenced owners’ attitudes, with those towards cat care practices varying more than those for dogs. The study highlights the need for responsible pet ownership interventions considering these demographic factors. The study identifies a knowledge gap among pet owners concerning the importance of regular veterinary visits and the impact of breeding for certain looks on animal welfare. The use of shock collar devices provoked varying opinions on their usage for training and behavior modification. The study suggests that enhancing pet owners’ knowledge is pivotal for responsible pet ownership. Overall, the findings emphasize the need for tailored interventions that account for demographic variations for promoting responsible pet ownership and animal welfare. The findings underscore the importance of improving access to veterinary health care teams, especially in rural areas, and of providing culturally appropriate education resources for both pet owners and veterinary health care teams. Full article