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Search Results (4,823)

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12 pages, 245 KiB  
Article
Examining the Relationship Between Increased Vegetable Consumption and Lifestyle Characteristics Among School-Aged Children: A Descriptive Study
by Konstantinos D. Tambalis, Dimitris Tampalis, Demosthenes B. Panagiotakos and Labros S. Sidossis
Appl. Sci. 2025, 15(15), 8665; https://doi.org/10.3390/app15158665 (registering DOI) - 5 Aug 2025
Abstract
The purpose of this study was to examine vegetable consumption and its relationship with lifestyle characteristics among children and adolescents. Data from a health survey administered to a representative sample of 177,091 schoolchildren between the ages of 8 and 17 were employed in [...] Read more.
The purpose of this study was to examine vegetable consumption and its relationship with lifestyle characteristics among children and adolescents. Data from a health survey administered to a representative sample of 177,091 schoolchildren between the ages of 8 and 17 were employed in this observational, cross-sectional investigation. Physical activity level, screen time, and sleeping patterns were assessed using self-completed questionnaires. Vegetable consumption and dietary habits were analyzed using the Mediterranean Diet Quality Index for Children and Adolescents. Participants consuming vegetables more than once daily were categorized as consumers vs. non-consumers. Physical education teachers measured anthropometric and physical fitness factors. Descriptive statistics and binary logistic regression analysis were conducted, and the odds ratio with the corresponding 95% confidence interval was calculated and adjusted for confounders. Vegetables were consumed once or more times a day by more females than males (25.5% vs. 24.0%, p < 0.001). In both sexes, vegetable consumers slept more, ate healthier, spent less time on screens, and had better anthropometric and aerobic fitness measurements than non-consumers. Healthy eating practices, such as regularly consuming fruits, legumes, nuts, and dairy products, were strongly correlated with vegetable intake. For every one-year increase in age, the odds of being a vegetable consumer decreased by 8% and 10% in boys and girls, respectively. Overweight/obese participants had lower odds of being a vegetable consumer by 20%. Increased screen time, inadequate physical activity, and insufficient sleeping hours decreased the odds of being a vegetable consumer by 22%, 30%, and 25%, respectively (all p-values < 0.001). Overall, a healthier lifestyle profile was associated with higher vegetable intake for both sexes among children and adolescents. Full article
(This article belongs to the Special Issue Potential Health Benefits of Fruits and Vegetables—4th Edition)
14 pages, 5995 KiB  
Article
Integrated Remote Sensing Evaluation of Grassland Degradation Using Multi-Criteria GDCI in Ili Prefecture, Xinjiang, China
by Liwei Xing, Dongyan Jin, Chen Shen, Mengshuai Zhu and Jianzhai Wu
Land 2025, 14(8), 1592; https://doi.org/10.3390/land14081592 - 4 Aug 2025
Abstract
As an important ecological barrier and animal husbandry resource base in arid and semi-arid areas, grassland degradation directly affects regional ecological security and sustainable development. Ili Prefecture is located in the western part of Xinjiang, China, and is a typical grassland resource-rich area. [...] Read more.
As an important ecological barrier and animal husbandry resource base in arid and semi-arid areas, grassland degradation directly affects regional ecological security and sustainable development. Ili Prefecture is located in the western part of Xinjiang, China, and is a typical grassland resource-rich area. However, in recent years, driven by climate change and human activities, grassland degradation has become increasingly serious. In view of the lack of comprehensive evaluation indicators and the inconsistency of grassland evaluation grade standards in remote sensing monitoring of grassland resource degradation, this study takes the current situation of grassland degradation in Ili Prefecture in the past 20 years as the research object and constructs a comprehensive evaluation index system covering three criteria layers of vegetation characteristics, environmental characteristics, and utilization characteristics. Net primary productivity (NPP), vegetation coverage, temperature, precipitation, soil erosion modulus, and grazing intensity were selected as multi-source indicators. Combined with data sources such as remote sensing inversion, sample survey, meteorological data, and farmer survey, the factor weight coefficient was determined by analytic hierarchy process. The Grassland Degeneration Comprehensive Index (GDCI) model was constructed to carry out remote sensing monitoring and evaluation of grassland degradation in Yili Prefecture. With reference to the classification threshold of the national standard for grassland degradation, the GDCI grassland degradation evaluation grade threshold (GDCI reduction rate) was determined by the method of weighted average of coefficients: non-degradation (0–10%), mild degradation (10–20%), moderate degradation (20–37.66%) and severe degradation (more than 37.66%). According to the results, between 2000 and 2022, non-degraded grasslands in Ili Prefecture covered an area of 27,200 km2, representing 90.19% of the total grassland area. Slight, moderate, and severe degradation accounted for 4.34%, 3.33%, and 2.15%, respectively. Moderately and severely degraded areas are primarily distributed in agro-pastoral transition zones and economically developed urban regions, respectively. The results revealed the spatial and temporal distribution characteristics of grassland degradation in Yili Prefecture and provided data basis and technical support for regional grassland resource management, degradation prevention and control and ecological restoration. Full article
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23 pages, 2193 KiB  
Article
A Virome Scanning of Saffron (Crocus sativus L.) at the National Scale in Iran Using High-Throughput Sequencing Technologies
by Hajar Valouzi, Akbar Dizadji, Alireza Golnaraghi, Seyed Alireza Salami, Nuria Fontdevila Pareta, Serkan Önder, Ilhem Selmi, Johan Rollin, Chadi Berhal, Lucie Tamisier, François Maclot, Long Wang, Rui Zhang, Habibullah Bahlolzada, Pierre Lefeuvre and Sébastien Massart
Viruses 2025, 17(8), 1079; https://doi.org/10.3390/v17081079 - 4 Aug 2025
Abstract
Saffron (Crocus sativus L.) is a vegetatively propagated crop of high economic and cultural value, potentially affected by viral infections that may impact its productivity. Despite Iran’s dominance in global saffron production, knowledge of its virome remains limited. In this study, we [...] Read more.
Saffron (Crocus sativus L.) is a vegetatively propagated crop of high economic and cultural value, potentially affected by viral infections that may impact its productivity. Despite Iran’s dominance in global saffron production, knowledge of its virome remains limited. In this study, we conducted the first nationwide virome survey of saffron in Iran employing a high-throughput sequencing (HTS) approach on pooled samples obtained from eleven provinces in Iran and one location in Afghanistan. Members of three virus families were detected—Potyviridae (Potyvirus), Solemoviridae (Polerovirus), and Geminiviridae (Mastrevirus)—as well as one satellite from the family Alphasatellitidae (Clecrusatellite). A novel Potyvirus, tentatively named saffron Iran virus (SaIRV) and detected in three provinces, shares less than 68% nucleotide identity with known Potyvirus species, thus meeting the ICTV criteria for designation as a new species. Genetic diversity analyses revealed substantial intrapopulation SNP variation but no clear geographical clustering. Among the two wild Crocus species sampled, only Crocus speciosus harbored turnip mosaic virus. Virome network and phylogenetic analyses confirmed widespread viral circulation likely driven by corm-mediated propagation. Our findings highlight the need for targeted certification programs and biological characterization of key viruses to mitigate potential impacts on saffron yield and quality. Full article
(This article belongs to the Special Issue Emerging and Reemerging Plant Viruses in a Changing World)
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14 pages, 3099 KiB  
Article
Identification of Keystone Plant Species for Avian Foraging and Nesting in Beijing’s Forest Ecosystems: Implications for Urban Forest Bird Conservation
by Lele Lin, Yongjian Zhao, Chao Yuan, Yushu Zhang, Siyu Qiu and Jixin Cao
Animals 2025, 15(15), 2271; https://doi.org/10.3390/ani15152271 - 4 Aug 2025
Viewed by 52
Abstract
Urban wildlife conservation is emerging as a critical component of sustainable city ecosystems. Rather than simply increasing tree abundance or species richness, conservation management should focus on key species. In this research, Xishan Forest Park in Beijing was chosen as a case study. [...] Read more.
Urban wildlife conservation is emerging as a critical component of sustainable city ecosystems. Rather than simply increasing tree abundance or species richness, conservation management should focus on key species. In this research, Xishan Forest Park in Beijing was chosen as a case study. Our aim was to identify keystone taxa critical for avian foraging and nesting during the breeding season. We performed a network analysis linking bird species, their diets, and nest plants. Dietary components were detected using DNA metabarcoding conducted with avian fecal samples. Nest plants were identified via transect surveys. Two indices of the network, degree and weighted mean degree, were calculated to evaluate the importance of the dietary and nest plant species. We identified 13 bird host species from 107 fecal samples and 14 bird species from 107 nest observations. Based on the degree indices, fruit trees Morus and Prunus were detected as key food sources, exhibiting both the highest degree (degree = 9, 9) and weighted mean degree (lnwMD = 5.21, 4.63). Robinia pseudoacacia provided predominant nesting sites, with a predominant degree of 7. A few taxa, such as Styphnolobium japonicum and Rhamnus parvifolia, served dual ecological significance as both essential food sources and nesting substrates. Scrublands, as a unique habitat type, provided nesting sites and food for small-bodied birds. Therefore, targeted management interventions are recommended to sustain or enhance these keystone resource species and to maintain the multi-layered vertical vegetation structure to preserve the diverse habitats of birds. Full article
(This article belongs to the Section Wildlife)
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23 pages, 1730 KiB  
Article
Prioritization and Sensitivity of Pesticide Risks from Root and Tuber Vegetables
by Milica Lučić and Antonije Onjia
J. Xenobiot. 2025, 15(4), 125; https://doi.org/10.3390/jox15040125 - 3 Aug 2025
Viewed by 378
Abstract
This study investigated pesticide residues in 580 vegetable samples collected from markets in Serbia, encompassing potatoes, carrots, celery, radishes, horseradish, ginger, onions, and leeks. In total, 33 distinct pesticides were detected using validated HPLC-MS/MS and GC-MS/MS analytical methods. Multiple residues were identified in [...] Read more.
This study investigated pesticide residues in 580 vegetable samples collected from markets in Serbia, encompassing potatoes, carrots, celery, radishes, horseradish, ginger, onions, and leeks. In total, 33 distinct pesticides were detected using validated HPLC-MS/MS and GC-MS/MS analytical methods. Multiple residues were identified in 19 samples, while 29 samples exceeded established maximum residue levels (MRLs). Acute and chronic dietary risks were assessed for both adults and children. Although individual hazard quotients (HQs) for adults and children remained below the threshold of concern (HQ < 1), the cumulative acute risk reached up to 63.1% of the Acute Reference Dose (ARfD) for children and 51.1% ARfD for adults, with ginger and celery posing the highest risks. Similarly, cumulative chronic risks remained below the safety threshold, with the Acceptable Daily Intake (ADI) percentages reaching a maximum of 5.9% ADI for adults and increased vulnerability of 11.0% ADI among children. Monte Carlo simulations were applied to account for variability and uncertainty in chronic exposure estimates. The hazard index (HI) results showed that adverse health effects for both population groups remained within acceptable safety limits (HI < 1), although higher susceptibility was observed in children. Sensitivity analysis identified body weight and vegetable consumption rates as the most influential factors affecting chronic risk variability. Full article
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30 pages, 4014 KiB  
Article
Spatial Heterogeneity in Carbon Pools of Young Betula sp. Stands on Former Arable Lands in the South of the Moscow Region
by Gulfina G. Frolova, Pavel V. Frolov, Vladimir N. Shanin and Irina V. Priputina
Plants 2025, 14(15), 2401; https://doi.org/10.3390/plants14152401 - 3 Aug 2025
Viewed by 104
Abstract
This study investigates the spatial heterogeneity of carbon pools in young Betula sp. stands on former arable lands in the southern Moscow region, Russia. The findings could be useful for the current estimates and predictions of the carbon balance in such forest ecosystems. [...] Read more.
This study investigates the spatial heterogeneity of carbon pools in young Betula sp. stands on former arable lands in the southern Moscow region, Russia. The findings could be useful for the current estimates and predictions of the carbon balance in such forest ecosystems. The research focuses on understanding the interactions between plant cover and the environment, i.e., how environmental factors such as stand density, tree diameter and height, light conditions, and soil properties affect ecosystem carbon pools. We also studied how heterogeneity in edaphic conditions affects the formation of plant cover, particularly tree regeneration and the development of ground layer vegetation. Field measurements were conducted on a permanent 50 × 50 m sampling plot divided into 5 × 5 m subplots, in order to capture variability in vegetation and soil characteristics. Key findings reveal significant differences in carbon stocks across subplots with varying stand densities and light conditions. This highlights the role of the spatial heterogeneity of soil properties and vegetation cover in carbon sequestration. The study demonstrates the feasibility of indirect estimation of carbon stocks using stand parameters (density, height, and diameter), with results that closely match direct measurements. The total ecosystem carbon stock was estimated at 80.47 t ha−1, with the soil contribution exceeding that of living biomass and dead organic matter. This research emphasizes the importance of accounting for spatial heterogeneity in carbon assessments of post-agricultural ecosystems, providing a methodological framework for future studies. Full article
(This article belongs to the Section Plant–Soil Interactions)
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12 pages, 2259 KiB  
Article
Soil C:N:P Stoichiometry in Two Contrasting Urban Forests in the Guangzhou Metropolis: Differences and Related Dominates
by Yongmei Xiong, Zhiqi Li, Shiyuan Meng and Jianmin Xu
Forests 2025, 16(8), 1268; https://doi.org/10.3390/f16081268 - 3 Aug 2025
Viewed by 133
Abstract
Carbon (C) sequestration and nitrogen (N) and phosphorus (P) accumulation in urban forest green spaces are significant for global climate regulation and alleviating nutrient pollution. However, the effects of management and conservation practices across different urban forest vegetation types on soil C, N, [...] Read more.
Carbon (C) sequestration and nitrogen (N) and phosphorus (P) accumulation in urban forest green spaces are significant for global climate regulation and alleviating nutrient pollution. However, the effects of management and conservation practices across different urban forest vegetation types on soil C, N, and P contents and stoichiometric ratios remain largely unexplored. We selected forest soils from Guangzhou, a major Metropolis in China, as our study area. Soil samples were collected from two urban secondary forests that naturally regenerated after disturbance (108 samples) and six urban forest parks primarily composed of artificially planted woody plant communities (72 samples). We employed mixed linear models and variance partitioning to analyze and compare soil C, N, and P contents and their stoichiometry and its main driving factors beneath suburban forests and urban park vegetation. These results exhibited that soil pH and bulk density in urban parks were higher than those in suburban forests, whereas soil water content, maximum storage capacity, and capillary porosity were higher in urban forests than in urban parks. Soil C, N, and P contents and their stoichiometry (except for N:P ratio) were significantly higher in suburban forests than in urban parks. Multiple analyzes showed that soil pH had the most pronounced negative influence on soil C, N, C:N, C:P, and N:P, but the strongest positive influence on soil P in urban parks. Soil water content had the strongest positive effect on soil C, N, P, C:N, and C:P, while soil N:P was primarily influenced by the positive effect of soil non-capillary porosity in suburban forests. Overall, our study emphasizes that suburban forests outperform urban parks in terms of carbon and nutrient accumulation, and urban green space management should focus particularly on the impact of soil pH and moisture content on soil C, N, and P contents and their stoichiometry. Full article
(This article belongs to the Special Issue Carbon, Nitrogen, and Phosphorus Storage and Cycling in Forest Soil)
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16 pages, 3523 KiB  
Article
Vegetation Composition and Environmental Relationships of Two Amaranthus Species Communities in Variant Agroecosystems at Fayoum Depression, Egypt
by Mai Sayed Fouad, Manar A. Megahed, Nabil A. Abo El-Kassem, Hoda F. Zahran and Abdel-Nasser A. A. Abdel-Hafeez
Diversity 2025, 17(8), 551; https://doi.org/10.3390/d17080551 - 3 Aug 2025
Viewed by 195
Abstract
Amaranthus is appointed as a common weed associated with crops. The research was designed to survey the Amaranth existence pattern throughout the Fayoum Depression, Egypt, accompanied with a community vegetation analysis. The study was extended to collect and analyze associated soil samples. The [...] Read more.
Amaranthus is appointed as a common weed associated with crops. The research was designed to survey the Amaranth existence pattern throughout the Fayoum Depression, Egypt, accompanied with a community vegetation analysis. The study was extended to collect and analyze associated soil samples. The obtained results figured out the prevalence of dicot families, herb growth forms, therophyte followed by phanerophyte life forms, the Pantropical monoregional chorotype, and the Mediterranean and Sudano-Zambezian followed by the Irano-Turanian pluri-regional chorotype. Multilevel pattern analysis stated that Gossypium barbadense, Corchorus olitorius, Sorghum bicolor, Sesamum indicum, and Zea mays are indicator species most related to Amaranth occurrence and prediction. NMDS analysis denoting that the Ibshaway, Youssef Al Seddik, Itsa, and Fayoum districts are the most representative districts for Amaranth existence on the basis of edaphic resources. Itsa and Youssef Al Seddik, in addition to Itsa and Fayoum, resemble each other in species composition. High pH and CaCO3 percentages were discriminatory in Ibshaway, Itsa, and Youssef Al Seddik. Ni was the cornerstone for districts partitioning in pruned trees. Finally, Amaranth was flourishing in both comfortable and harsh habitats with cultivated crops and orchards, as well as on the outskirts. The findings are considered to be valorized by decision makers in arable land management. Full article
(This article belongs to the Section Plant Diversity)
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25 pages, 28131 KiB  
Article
Landslide Susceptibility Assessment in Ya’an Based on Coupling of GWR and TabNet
by Jiatian Li, Ruirui Wang, Wei Shi, Le Yang, Jiahao Wei, Fei Liu and Kaiwei Xiong
Remote Sens. 2025, 17(15), 2678; https://doi.org/10.3390/rs17152678 - 2 Aug 2025
Viewed by 359
Abstract
Landslides are destructive geological hazards, making accurate landslide susceptibility assessment essential for disaster prevention and mitigation. However, existing studies often lack scientific rigor in negative sample construction and have unclear model applicability. This study focuses on Ya’an City, Sichuan Province, China, and proposes [...] Read more.
Landslides are destructive geological hazards, making accurate landslide susceptibility assessment essential for disaster prevention and mitigation. However, existing studies often lack scientific rigor in negative sample construction and have unclear model applicability. This study focuses on Ya’an City, Sichuan Province, China, and proposes an innovative approach to negative sample construction using Geographically Weighted Regression (GWR), which is then integrated with Tabular Network (TabNet), a deep learning architecture tailored to structured tabular data, to assess landslide susceptibility. The performance of TabNet is compared against Random Forest, Light Gradient Boosting Machine, deep neural networks, and Residual Networks. The experimental results indicate that (1) the GWR-based sampling strategy substantially improves model performance across all tested models; (2) TabNet trained using the GWR-based negative samples achieves superior performance over all other evaluated models, with an average AUC of 0.9828, exhibiting both high accuracy and interpretability; and (3) elevation, land cover, and annual Normalized Difference Vegetation Index are identified as dominant predictors through TabNet’s feature importance analysis. The results demonstrate that combining GWR and TabNet substantially enhances landslide susceptibility modeling by improving both accuracy and interpretability, establishing a more scientifically grounded approach to negative sample construction, and providing an interpretable, high-performing modeling framework for geological hazard risk assessment. Full article
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22 pages, 8105 KiB  
Article
Extraction of Sparse Vegetation Cover in Deserts Based on UAV Remote Sensing
by Jie Han, Jinlei Zhu, Xiaoming Cao, Lei Xi, Zhao Qi, Yongxin Li, Xingyu Wang and Jiaxiu Zou
Remote Sens. 2025, 17(15), 2665; https://doi.org/10.3390/rs17152665 - 1 Aug 2025
Viewed by 200
Abstract
The unique characteristics of desert vegetation, such as different leaf morphology, discrete canopy structures, sparse and uneven distribution, etc., pose significant challenges for remote sensing-based estimation of fractional vegetation cover (FVC). The Unmanned Aerial Vehicle (UAV) system can accurately distinguish vegetation patches, extract [...] Read more.
The unique characteristics of desert vegetation, such as different leaf morphology, discrete canopy structures, sparse and uneven distribution, etc., pose significant challenges for remote sensing-based estimation of fractional vegetation cover (FVC). The Unmanned Aerial Vehicle (UAV) system can accurately distinguish vegetation patches, extract weak vegetation signals, and navigate through complex terrain, making it suitable for applications in small-scale FVC extraction. In this study, we selected the floodplain fan with Caragana korshinskii Kom as the constructive species in Hatengtaohai National Nature Reserve, Bayannur, Inner Mongolia, China, as our study area. We investigated the remote sensing extraction method of desert sparse vegetation cover by placing samples across three gradients: the top, middle, and edge of the fan. We then acquired UAV multispectral images; evaluated the applicability of various vegetation indices (VIs) using methods such as supervised classification, linear regression models, and machine learning; and explored the feasibility and stability of multiple machine learning models in this region. Our results indicate the following: (1) We discovered that the multispectral vegetation index is superior to the visible vegetation index and more suitable for FVC extraction in vegetation-sparse desert regions. (2) By comparing five machine learning regression models, it was found that the XGBoost and KNN models exhibited relatively lower estimation performance in the study area. The spatial distribution of plots appeared to influence the stability of the SVM model when estimating fractional vegetation cover (FVC). In contrast, the RF and LASSO models demonstrated robust stability across both training and testing datasets. Notably, the RF model achieved the best inversion performance (R2 = 0.876, RMSE = 0.020, MAE = 0.016), indicating that RF is one of the most suitable models for retrieving FVC in naturally sparse desert vegetation. This study provides a valuable contribution to the limited existing research on remote sensing-based estimation of FVC and characterization of spatial heterogeneity in small-scale desert sparse vegetation ecosystems dominated by a single species. Full article
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26 pages, 3030 KiB  
Article
Predicting Landslide Susceptibility Using Cost Function in Low-Relief Areas: A Case Study of the Urban Municipality of Attecoube (Abidjan, Ivory Coast)
by Frédéric Lorng Gnagne, Serge Schmitz, Hélène Boyossoro Kouadio, Aurélia Hubert-Ferrari, Jean Biémi and Alain Demoulin
Earth 2025, 6(3), 84; https://doi.org/10.3390/earth6030084 (registering DOI) - 1 Aug 2025
Viewed by 216
Abstract
Landslides are among the most hazardous natural phenomena affecting Greater Abidjan, causing significant economic and social damage. Strategic planning supported by geographic information systems (GIS) can help mitigate potential losses and enhance disaster resilience. This study evaluates landslide susceptibility using logistic regression and [...] Read more.
Landslides are among the most hazardous natural phenomena affecting Greater Abidjan, causing significant economic and social damage. Strategic planning supported by geographic information systems (GIS) can help mitigate potential losses and enhance disaster resilience. This study evaluates landslide susceptibility using logistic regression and frequency ratio models. The analysis is based on a dataset comprising 54 mapped landslide scarps collected from June 2015 to July 2023, along with 16 thematic predictor variables, including altitude, slope, aspect, profile curvature, plan curvature, drainage area, distance to the drainage network, normalized difference vegetation index (NDVI), and an urban-related layer. A high-resolution (5-m) digital elevation model (DEM), derived from multiple data sources, supports the spatial analysis. The landslide inventory was randomly divided into two subsets: 80% for model calibration and 20% for validation. After optimization and statistical testing, the selected thematic layers were integrated to produce a susceptibility map. The results indicate that 6.3% (0.7 km2) of the study area is classified as very highly susceptible. The proportion of the sample (61.2%) in this class had a frequency ratio estimated to be 20.2. Among the predictive indicators, altitude, slope, SE, S, NW, and NDVI were found to have a positive impact on landslide occurrence. Model performance was assessed using the area under the receiver operating characteristic curve (AUC), demonstrating strong predictive capability. These findings can support informed land-use planning and risk reduction strategies in urban areas. Furthermore, the prediction model should be communicated to and understood by local authorities to facilitate disaster management. The cost function was adopted as a novel approach to delineate hazardous zones. Considering the landslide inventory period, the increasing hazard due to climate change, and the intensification of human activities, a reasoned choice of sample size was made. This informed decision enabled the production of an updated prediction map. Optimal thresholds were then derived to classify areas into high- and low-susceptibility categories. The prediction map will be useful to planners in helping them make decisions and implement protective measures. Full article
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12 pages, 1161 KiB  
Article
Power Ultrasound and Organic Acid-Based Hurdle Technology to Reduce Listeria monocytogenes and Salmonella enterica on Fresh Produce
by Megan L. Fay, Priya Biswas, Xinyi Zhou, Bashayer A. Khouja, Diana S. Stewart, Catherine W. Y. Wong, Wei Zhang and Joelle K. Salazar
Microbiol. Res. 2025, 16(8), 172; https://doi.org/10.3390/microbiolres16080172 - 1 Aug 2025
Viewed by 142
Abstract
The increasing demand for fresh fruits and vegetables has been accompanied by a rise in foodborne illness outbreaks linked to fresh produce. Traditional antimicrobial washing treatments, such as chlorine and peroxyacetic acid, have limitations in efficacy and pose environmental and worker health concerns. [...] Read more.
The increasing demand for fresh fruits and vegetables has been accompanied by a rise in foodborne illness outbreaks linked to fresh produce. Traditional antimicrobial washing treatments, such as chlorine and peroxyacetic acid, have limitations in efficacy and pose environmental and worker health concerns. This study evaluated the effectiveness of organic acids (citric, malic, and lactic acid) and power ultrasound, individually and in combination, for the reduction in Salmonella enterica and Listeria monocytogenes on four fresh produce types: romaine lettuce, cucumber, tomato, and strawberry. Produce samples were inoculated with bacterial cocktails at 8–9 log CFU/unit and treated with organic acids at 2 or 5% for 2 or 5 min, with or without power ultrasound (40 kHz). Results showed that pathogen reductions varied based on the produce matrix with smoother surfaces such as tomato, exhibiting greater reductions than rougher surfaces (e.g., romaine lettuce and strawberry). Lactic and malic acids were the most effective treatments, with 5% lactic acid achieving a reduction of >5 log CFU/unit for S. enterica and 4.53 ± 0.71 log CFU/unit for L. monocytogenes on tomatoes. The combination of organic acids and power ultrasound demonstrated synergistic effects, further enhancing pathogen reduction by <1.87 log CFU/unit. For example, S. enterica on cucumbers was reduced by an additional 1.87 log CFU/unit when treated with 2% malic acid and power ultrasound for 2 min compared to malic acid alone. Similarly, L. monocytogenes on strawberries was further reduced by 1.84 log CFU/unit when treated with 5% malic acid and power ultrasound for 2 min. These findings suggest that organic acids, particularly malic and lactic acids, combined with power ultrasound, may serve as an effective hurdle technology for enhancing the microbial safety of fresh produce. Future research can include validating these treatments in an industrial processing environment. Full article
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21 pages, 2608 KiB  
Article
Quality and Quantity Losses of Tomatoes Grown by Small-Scale Farmers Under Different Production Systems
by Tintswalo Molelekoa, Edwin M. Karoney, Nazareth Siyoum, Jarishma K. Gokul and Lise Korsten
Horticulturae 2025, 11(8), 884; https://doi.org/10.3390/horticulturae11080884 (registering DOI) - 1 Aug 2025
Viewed by 182
Abstract
Postharvest losses amongst small-scale farmers in developing countries are high due to inadequate resources and infrastructure. Among the various affected crops, tomatoes are particularly vulnerable; however, studies on postharvest losses of most fruits and vegetables are limited. Therefore, this study aimed to assess [...] Read more.
Postharvest losses amongst small-scale farmers in developing countries are high due to inadequate resources and infrastructure. Among the various affected crops, tomatoes are particularly vulnerable; however, studies on postharvest losses of most fruits and vegetables are limited. Therefore, this study aimed to assess postharvest tomato losses under different production systems within the small-scale supply chain using the indirect assessment (questionnaires and interviews) and direct quantification of losses. Farmers reported tomato losses due to insects (82.35%), cracks, bruises, and deformities (70.58%), and diseases (64.71%). Chemical sprays were the main form of pest and disease control reported by all farmers. The direct quantification sampling data revealed that 73.07% of the tomatoes were substandard at the farm level, with 47.92% and 25.15% categorized as medium-quality and poor-quality, respectively. The primary contributors to the losses were decay (39.92%), mechanical damage (31.32%), and blotchiness (27.99%). Postharvest losses were significantly higher under open-field production systems compared to closed tunnels. The fungi associated with decay were mainly Geotrichum, Fusarium spp., and Alternaria spp. These findings demonstrate the main drivers behind postharvest losses, which in turn highlight the critical need for intervention through training and support, including the use of postharvest loss reduction technologies to enhance food security. Full article
(This article belongs to the Section Postharvest Biology, Quality, Safety, and Technology)
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16 pages, 1047 KiB  
Article
The Post-Harvest Application of UV-C Rays: Effects on the Shelf Life and Antioxidants of Fresh Green Asparagus (Asparagus officinalis L.)
by Valeria Menga, Romina Beleggia, Domenico Pio Prencipe, Mario Russo and Clara Fares
Appl. Sci. 2025, 15(15), 8533; https://doi.org/10.3390/app15158533 (registering DOI) - 31 Jul 2025
Viewed by 104
Abstract
UV-C irradiation is an innovative postharvest technique for increasing the safety of fruits and vegetables. This study investigated the effect of UV-C rays (UV-C1 = 0.26 KJ/m2; UV-C2 = 0.40 KJ/m2; UV-C3 = 0.67 KJ/m2; and UV-C4 [...] Read more.
UV-C irradiation is an innovative postharvest technique for increasing the safety of fruits and vegetables. This study investigated the effect of UV-C rays (UV-C1 = 0.26 KJ/m2; UV-C2 = 0.40 KJ/m2; UV-C3 = 0.67 KJ/m2; and UV-C4 = 1.34 KJ/m2) on the preservation of the antioxidants, hardness, and color of fresh green asparagus during storage. UV-C1 and UV-C2 significantly maintained higher total phenolic content (10.6%), total flavonoid content (36%), rutin (14.3%), quercetin (27.03%), kaempferol-3-O-rutinoside (21.25%), and antioxidant activity (DPPH 7.5%). Over three weeks of storage, quercetin, ferulic acid, and kaempferol 3-O-rutinoside increased, while rutin and caffeic acid decreased. Storage caused a significant change in the color and hardness of the control sample, but UV-C4 counteracted hardening for up to three weeks, and UV-C3 was the best dose for stabilizing color during storage. This study indicates that the choice of UV-C dose can be modulated based on the characteristics that are intended to be preserved in green asparagus, maintaining a balance between nutraceutical and hedonic characteristics. To maintain the maximum level of nutraceutical compounds over time, UV-C2 can be adopted, while to preserve texture and color, UV-C3 and UV-C4 are a better choice. Full article
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7 pages, 2626 KiB  
Proceeding Paper
SpaFLEX: Field Campaign for Calibration and Validation of FLEX-S3 Mission Products
by Pedro J. Gómez-Giráldez, David Aragonés, Marcos Jiménez, Mª Pilar Cendrero-Mateo, Shari Van Wittenberghe, Juan José Peón, Adrián Moncholí-Estornell and Ricardo Díaz-Delgado
Eng. Proc. 2025, 94(1), 13; https://doi.org/10.3390/engproc2025094013 - 31 Jul 2025
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Abstract
The FLEX-S3 mission by ESA will deliver key Level 2 products such as sun-induced chlorophyll fluorescence (SIF) and vegetation-reflected radiance. To validate these, the SpaFLEX project, funded by the Spanish Ministry of Science and Innovation, is developing a robust calibration and validation strategy [...] Read more.
The FLEX-S3 mission by ESA will deliver key Level 2 products such as sun-induced chlorophyll fluorescence (SIF) and vegetation-reflected radiance. To validate these, the SpaFLEX project, funded by the Spanish Ministry of Science and Innovation, is developing a robust calibration and validation strategy in Spain. This includes test site setup, instrument characterization, and sampling protocols. A field campaign was conducted in two Holm Oak forests in Teruel, analyzing Sentinel-2 spatial heterogeneity and collecting ground, UAV, and airborne data. The results support scaling procedures to match the 300 m pixel resolution of FLEX-S3, ensuring product accuracy and compliance with ESA standards. Full article
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