Search Results (82)

In this study, we investigated the chemical composition, antimicrobial and antibiofilm activities, and cytotoxicity of Thuja occidentalis L. (thuja) and Myrtus communis L. (myrtle) essential oils (EOs) to evaluate their potential as natural alternatives for topical applications. Thuja EOs were extracted from fresh and dried leaves and cones, while myrtle EO was extracted from fresh leaves. GC-MS analysis revealed that myrtle EO was rich in oxygenated monoterpenes (58.2%), predominantly eucalyptol (41.86%) and α-pinene (20.98%). In contrast, thuja EOs were dominated by monoterpene hydrocarbons (55–82%), with α-pinene as the major component (29–66%), and their composition varied markedly with plant organ and moisture state. Myrtle EO displayed the most potent and broad-spectrum antimicrobial activity, with MIC values as low as 3.096 µL/mL against Staphylococcus aureus and Escherichia coli, and effectively inhibited microbial adherence. Thuja EOs showed selective activity, particularly against Gram-positive bacteria and Candida parapsilosis, with EOs extracted from fresh leaves and cones exhibiting the lowest MICs (1.95–7.8 µL/mL). All EOs did not show cytotoxicity to human keratinocytes at concentrations ≤125 µg/mL and, when diluted to 0.05%, indicated excellent skin compatibility in human patch tests. This study suggests that myrtle and thuja EOs, particularly myrtle, are promising and safe natural antimicrobial agents for dermatological and cosmetic formulations. Full article

In addition to the true cedars (Cedrus species), there are several genera of trees commonly called “cedar”, including species of Callitropsis, Calocedrus, Cedrela, Chamaecyparis, Cryptomeria, Cupressus, Juniperus, Thuja, and Widdringtonia. The wood essential oils (cedarwood oils) of these trees have been used as flavor and fragrance materials, as well as in medicinal applications. In this study, we present summaries of the wood essential oils from trees known as “cedar”. A literature search was carried out on cedarwood oils and, when available, compared with commercial wood essential oils from the Aromatic Plant Research Center (APRC) collection. Cedrus wood oils are generally dominated by the himachalenes and atlantones. Sesquiterpenoids are abundant in other cedarwood oils, including cedrenes, cedrol, and thujopsene in Cupressus funebris, Juniperus ashei, and Juniperus virginiana. Cadinane sesquiterpenoids are generally abundant in Cedrela odorata and Cryptomeria japonica, while nootkatane sesquiterpenoids are found in Callitropsis nootkatensis and eudesmane sesquiterpenoids are found in Thuja occidentalis. Sesquiterpenoids are generally responsible for the woody fragrances of cedarwood oils, but monoterpenoids can also be dominant (e.g., Calocedrus species). Full article

Thuja arborvitae are widely grown in North America and East Asia for their ornamental value, and their leaf oil extracts have been used to treat bacterial infections. This study aimed to identify antibacterial compounds from Thuja leaves. The methanol extract of Thuja leaves exhibited strong antibacterial activity against Gram-positive (Staphylococcus aureus and Streptococcus mutans) and Gram-negative (Acinetobacter baumannii, Pseudomonas aeruginosa) bacteria. The major compounds in the active fractions were isolated and identified as apigenin-7-di-p-coumarylglucoside and eicosapentaenoic acid. The identified compounds showed potent antibacterial activity against the four tested microorganisms with IC50 values of 10 to 50 µg/mL. More importantly, these compounds showed potent inhibitory activity (IC50: 10 μg/mL) against the multidrug-resistant bacterial strain Acinetobacter baumannii. Two antibacterial compounds are now being reported for the first time in Thuja arborvitae, and they may have potential for the treatment of bacterial infections. Full article

Tree pollen allergies are influenced by regional atmospheric pollen concentrations and flora distribution. Climate change and urban landscaping have altered airborne pollen profiles in Ukraine, potentially affecting sensitization patterns. We examined 7518 patients (57.63% children) sensitized to at least one of 26 molecular components from 19 tree species using ALEX testing (2020–2022). Atmospheric pollen data from Ukrainian aerobiology stations were integrated with clinical data. Regional sensitization was mapped using the Geographic Information System, and Bayesian network modeling determined hierarchical relationships. Sensitization to Cry j 1 (46.01%), Bet v 1 (41.67%), and Fag s 1 (34.38%) dominated across age groups. High Fagales sensitization correlated with elevated atmospheric Betula, Alnus, and Corylus pollen concentrations, confirming environmental exposure-sensitization relationships. Bayesian modeling identified Bet v 1 as the root allergen (89.43% accuracy) driving cascading sensitization to other Fagales and non-Fagales allergens. Unexpectedly high Cry j 1 sensitization despite minimal atmospheric Cryptomeria presence suggests Thuja and Ambrosia cross-reactivity. Fagales sensitization dominated 10 of 17 regions, correlating with forest geography and urban landscaping. This study validates aerobiological monitoring’s clinical relevance. Diagnostic protocols should prioritize Bet v 1 while interpreting Cry j 1 positivity as potential cross-reactivity. Climate-driven shifts in atmospheric pollen patterns require ongoing coordinated aerobiological and clinical surveillance. Full article

Atopic dermatitis (AD) is a chronic inflammatory skin disorder characterized by dysregulated immunity, skin barrier dysfunction, and cutaneous microbiome dysbiosis. While current therapies face limitations, Thuja sutchuenensis essential oil (TEO) shows promise due to its multi-target potential. We sought to explore the beneficial effects of TEO and delve into its mechanistic actions in a mouse model of AD. We combined network pharmacology with in vivo validation to evaluate the therapeutic efficacy and mechanisms of TEO in an AD model, and confirmed network-predicted targets in an in vitro inflammatory cell model. In AD mice, TEO alleviated pruritus and epidermal hyperplasia, suppressed systemic IL-4/TNF-α and IgE, and partially normalized serum ALB, LDL-C, and HDL-C. Microbial diversity increased after treatment, although potentially pathogenic taxa (Arthrobacter sp. and Corynebacterium mastitidis) remained enriched. Machine-learning analysis indicated the highest predicted metabolic activity in CK controls, whereas the AD and TEO groups showed elevated pathogenic phenotype scores. Network pharmacology prioritized active compounds [(E)-ligustilide, senkyunolide A, 3-butylisobenzofuran-1(3H)-one, butylated hydroxytoluene, Z-buthlidenephthalide, and β-Myrcene] and core targets (TNF, PTPRC, CCR5, JAK1), implicating T-cell receptor signaling, Staphylococcus aureus infection, and STAT3 pathways. Docking and molecular dynamics supported strong, stable binding of major constituents to JAK1, and Western blotting confirmed TEO-mediated inhibition of the JAK1/STAT3 axis. TEO effectively alleviates atopic dermatitis symptoms by modulating immune responses and enhancing microbial diversity. It targets key signaling pathways, such as JAK1/STAT3, highlighting its potential as a therapeutic option for AD. Full article

Sarcocystis is a genus of heteroxenous, globally distributed coccidian parasites. Limited research has been conducted on the natural infection of Sarcocystis in rodents in the Middle East. In this study, the Libyan jird (Meriones libycus) was identified as the natural intermediate host of the new species Sarcocystis meriones, based on morphological and molecular data. Microscopic sarcocysts were detected in the thigh muscles of 8.5% (4/47) of Libyan jirds captured from a semi-desert area in Amghara, Eastern Kuwait. Under the light microscope, sarcocysts were filamentous with blunt ends and thin walls, measuring 1550 × 89 µm. Transmission electron microscopy analysis showed the densely packed protrusions measure 1.2 × 0.5 µm and resemble thuja or a cylinder and having lateral microvilli, while the ground substance layer was 0.5–0.6 µm thick and type 22-like. Based on four genetic loci (18S rRNA, 28S rRNA, ITS1, and cox1), S. meriones was genetically most similar to S. myodes and S. ratti, infecting voles and mice of the genus Apodemus and black rats (Rattus rattus), respectively. Phylogenetic results suggest predatory mammals as potential definitive hosts of S. meriones. Further studies are needed to reveal host specificity, geographical distribution, and the impact of the parasite on the host’s health of the newly described Sarcocystis species. Full article

Combining products of natural origin with different mechanisms of action on insect herbivores may provide an alternative among methods of plant protection against pests that are less risky for the environment. The aim of the study was to evaluate the effectiveness of mixtures of Thuja occidentalis L. essential oil and diatomite (EO + DE) compared to each substance separately in reducing economically important pests such as black bean aphid (BBA) Aphis fabae Scop., Colorado potato beetle (CPB) Leptinotarsa decemlineata Say., and pea leaf weevil (PLW) Sitona lineatus L. The effects on mortality (all pests) and foraging intensity (CPB and PLW) were tested. The improvement in effectiveness using a mixture of EO + DE versus single components against BBA was dose- and the developmental stage-dependent. The effect of enhancing CPB foraging inhibition through DE addition was obtained at a concentration of 0.2% EO (both females and males of CPB) and 0.5% EO (males) in no-choice experiments. In choice experiments, mixtures EO + DE with both 0.2% and 0.5% EO concentrations resulted in a significant reduction in CPB foraging. A significant strengthening effect of EO 0.5% through the addition of DE at a dose of 10% against PLW males was observed in the no-choice experiment, while, when the beetles had a choice, the synergistic effect of a mixture of EO 0.5% and DE 10% was also apparent in females. In conclusion, the use of DE mixtures with EO from T. occidentalis appears to be a promising strategy. The results support the idea of not using doses of EO higher than 0.5%. Full article

Endemic plant species, with their restricted distribution, are vulnerable to extinction due to human activities and environmental change. Monitoring their ecological characteristics and habitat relationships is crucial for conservation. This study examined plant communities to prioritize populations for conserving the Korean endemic species, Taihyun’s abelia (Zabelia tyaihyonii (Nakai) Hisauti & H.Hara), and to identify threats and strategies for its protection. Vegetation surveys were conducted, classifying communities and analyzing species composition differences. Habitat quality and zeta diversity, assessed using the InVEST model, identified three community types: Quercus dentata–Thuja orientalis (Com. 1), Fraxinus rhynchophylla–Buxus koreana (Com. 2), and Quercus dentata–Carex humilis var. nana (Com. 3). Community classification was supported by a multi-response permutation procedure (p < 0.001) and non-metric multidimensional scaling (R² = 0.643). Species richness and soil calcium influenced species composition, and habitat quality was moderate (0.5562 ± 0.0294). Com. 1 and Com. 3 showed minimal zeta diversity decline, indicating strong habitat connectivity. However, fluctuations at zeta orders 8–12 suggested localized disturbances. Species turnover instability was linked to urbanization and disturbance. This study, using a diverse set of analytical tools, was able to pinpoint key features of habitat quality and composition associated with Z. tyaihyonii and the anthropogenic factors that will lead to its decline. Our work provides a road map for the conservation of other rare and endemic Korean plant species with similar conservation issues. Full article

The World Health Organization (WHO) has recognized Particulate Matter (PM) as the main threat to human health from air pollution. One of the solutions is Green Infrastructure (GI), which uses different plants to mitigate pollution. Among these plants are bryophytes (or more commonly used mosses), which have easier maintenance, lighter weight, and durability compared to vascular plants. However, currently, there is limited knowledge of its effectiveness in air pollution mitigation. By addressing this gap in current scientific knowledge, more effective deployment of GI could be introduced by municipalities for society’s health benefits. This study aimed to evaluate three species of mosses (Dicranum scoparium, Plagiomnium affine, and Hypnum cupressiforme) and one thuja (Thuja plicata) as a control species for a possible GI vertical barrier for local de-pollution. The objective was to assess different moss species’ effectiveness in air pollution PM_2.5 and PM₁₀ absorption in a laboratory setting. The practical experiment was conducted from June–July 2024 in the Laboratory of the Physics and Chemistry of Environment and Space in Orleans (LPC2E-CNRS), France. For the experiment, a unique air pollution chamber was engineered and built with a linear barrier of GI inside to measure pollution absorption before and after the barrier. With the obtained data from the sensors, the efficiency of the vegetation barrier was calculated. The total average efficiency of all 18 tests and tested moss species is 41% for PM_2.5 and 47% for PM₁₀ mass concentrations. Efficiency shows moss species’ maximum or optimal ability to absorb pollution PM_2.5 and PM₁₀ in laboratory environments, with the limitations indicated in this article. This research is an essential step towards further and more profound research on the effectiveness of GI barriers of mosses in urban environments. It significantly contributes to understanding GI effects on air pollution and presents the results for specific moss species and their capacity for PM_2.5 and PM₁₀ mitigation in the air. The novelty of the study lies in a particular application of the chosen moss species. Full article

Alternaria is a genus that contains several important plant pathogens affecting nearly 400 plant species worldwide, including economically important crops such as grapes, citrus, and ornamental plants. Rapid, scalable, and efficient methods of pathogen detection are crucial for managing plant diseases and ensuring agricultural productivity. Current amplicon sequencing protocols for Alternaria detection often require the enzymatic barcoding of amplicons, increasing hands-on time, cost, and contamination risk. We present a proof-of-concept study using custom barcoded primers, combining universal primers targeting ITS1 and ITS2 regions (600 bp) coupled with Oxford Nanopore Technologies (ONT) barcode sequences. Sequencing was performed on infected grapevine, mandarin orange, thuja, and maple tree samples. In total, we analyzed 38 samples using qPCR; 8 tested positive for Alternaria, which were sequenced using a newly developed protocol. As a result, we could identify Alternaria in every positive sample, and besides the pathogen of interest, we could identify the associated mycobiome. This protocol reduces hands-on time and cost, making a significant advancement over current sequencing methods. Future work will focus on optimizing our approach for high-throughput sequencing of up to 96 samples and determining the method’s applicability for large-scale mycobiome analysis. Full article

Background/Objectives: Hair loss affects self-esteem, confidence, and psychological well-being. Exosomes, as molecular carriers of growth factors and active compounds, offer a promising treatment. This study evaluates the efficacy of an exosome formulation containing extracts from two known hair-regenerating plants, Ecklonia cava and Thuja orientalis (ECPE), for male pattern alopecia. Methods: A randomized controlled trial included 20 male participants with Norwood grade 2–3 androgenetic alopecia who were randomly assigned into two groups, placebo (0.9% sodium chloride) and ECPE, administered bi-weekly across four sessions. Evaluations included hair density measurements, adverse effect tracking, and self-assessments. Results: Most participants (55%) were aged 18 to 35, with 75% reporting hair loss for over a year and 80% noting scalp thinning. The hair counts showed no significant change in the placebo group from baseline to week 16 (Wilcoxon signed-rank test: V = 13.5, p = 0.163), while a significant increase was observed in the ECPE group (V = 0, p = 0.002). Between-group analysis revealed a significant difference in the hair count changes (Wilcoxon rank-sum test: W = 86.5, p = 0.006) with a large effect size (Cliff’s Delta: & = 0.73, 95% CI: 0.41–0.89), with the ECPE group showing higher median hair growth (9.5, IQR = 16.88) compared to the placebo group (1.5, IQR = 3.00). A Bayesian ANCOVA, adjusted for covariates (the father’s scalp hair condition, baseline hair count, and Norwood classification), showed no significant effect of these factors on the outcomes. Conclusions: These findings suggest that ECPE significantly improves hair regrowth compared to the placebo, with no notable adverse effects. Full article

The evergreen coniferous tree Thuja occidentalis is a member of the Cupressaceae family. This study included biological, cytotoxic, and in silico docking analyses in addition to a phytochemical composition analysis of the plant leaves and stem ethanolic extracts. The extracts’ in vitro cytotoxicity efficacy against various cancer cell lines was examined. Additionally, certain phytochemical compounds were identified by gas chromatographic analysis and subsequently assessed in silico against anticancer molecular targets. Also, their antiviral effect was assessed. Good cytotoxic activity was demonstrated by plant extracts against the lung and colorectal cancer cell lines. With half-maximal inhibitory concentration values of 18.45 μg/mL for the leaf extract and 33.61 μg/mL for the stem extract, apoptosis and S-phase arrest was observed in the lung cancer cell line. In addition, the leaf extract demonstrated effective antiviral activity, with suppression rates of 17.7 and 16.2% for the herpes simplex and influenza viruses, respectively. Gas chromatographic analysis revealed the presence of relevant bioactive components such as Podocarp-7-en-3β-ol, 13β-methyl-13-vinyl, Megastigmatrienone, and Cedrol, which were tested in silico against anticancer molecular targets. Our findings suggest that plant ethanolic extracts may have potential therapeutic uses as anticancer drugs against lung cancer in addition to their antiviral properties, which opens up further avenues for more investigation and applications. Full article

Background: (-)-Fenchone is a bicyclic monoterpene present in the plant species Foeniculum vulgare Mill, Thuja occidentalis L. (tuja), and Lavandula stoechas (lavender). These plants have therapeutic value in the treatment of intestinal disorders. Aim: To evaluate intestinal anti-inflammatory activity in an acute and chronic trinitrobenzene sulphonic acid (TNBS)-induced colitis model in rats. Methods: Intestinal anti-inflammatory effects were assessed using the acute and chronic TNBS-induced colitis model in rats. The mechanisms were evaluated from colonic tissue fragments of the acute and chronic models. Results: Oral administration of the (-)-fenchone (37.5–300 mg/kg) acute phase or (150 mg/kg) (p < 0.001) chronic phase reduced the macroscopic lesion score, ulcerative area, intestinal weight/length ratio, and diarrheal index in TNBS-treated animals. At a dose of 150 mg/kg, the acute and chronic phase decreased malondialdehyde (MDA) and myeloperoxidase (MPO) (p < 0.001), restored glutathione (GSH) levels and superoxide dismutase (SOD) (p < 0.001), decreased immunomarking for factor nuclear kappa B (NF-κB) and levels of interleukin (IL)-1 and tumor necrosis factor α (TNF-α), and maintained IL-10 and TGF-β basal levels. Furthermore, increased immunostaining for zonula occludens 1 (ZO-1) was observed. Conclusions: (-)-fenchone has intestinal anti-inflammatory activity related to cytoprotection of the intestinal barrier, as well as antioxidant and immunomodulatory effects. Full article

Air pollution, including particulate matter (PM), impacts public health in urban areas. Vegetation acts as a natural filter, removing environmental pollution by absorbing large quantities of toxic substances on the foliage. Ambient air pollution problems are real in Armenia’s cities. This article presents the results of a study based on field sampling in July 2022 undertaken in urban parks and streets in the Armenian cities of Yerevan, Gyumri, and Vanadzor. The three cities have different climates and geographic conditions. The main research goal was a comparative study of the accumulation of PM by urban greenery. The most widespread tree species were selected for the study in each city: in Yerevan, Platanus orientalis and Quercus robur; in Gyumri, Fraxinus excelsior and Tilia caucasica; and in Vanadzor, Aesculus hippocastanum and Acer pseudoplatanus. The ecological status of trees was assessed through visual observation. Tree species with high PM uptake potential were identified and selected for inclusion in urban greening systems (Platanus orientalis, Fraxinus excelsior, and Quercus robur in Yerevan; Tilia caucasica, Sorbus persica, Fraxinus excelsior, and Populus alba in Grumri; Acer pseudoplatanus, Fraxinus excelsior, Aesculus hippocastanum, and Thuja occidentalis in Vanadzor.). High PM accumulation was found on the leaves of tree species in all of the investigated cities, with the largest amount recorded in Yerevan. In these cities, PM levels were higher in street plantations than in parks. All studied tree species have a high potential for PM absorption, demonstrating strong phytofilter properties. Therefore, they can be effectively used in their typical climatic zones and included in street plantings, gardens, and parks. These results can help urban planners and policymakers make informed decisions about urban greening initiatives to improve air quality and overall wellbeing. Full article

An estimated 2000 plant species have been employed for pest control worldwide. The use of these botanical derivatives is thought to be one of the most cost-effective and sustainable options for pest management in stored grain. The present study was designed to assess the efficacy of five plant extracts viz; Nicotiana tabacum L., Nicotiana rustica L., Azadirachta indica A. Juss., Thuja orientalis L., and Melia azedarach L. against Callosobruchus maculatus L. Plant species extracts were applied at six different concentrations, i.e., 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0% in four replications. The phytochemical analyses of ethanolic extracts of five plant species showed variable amounts of phytochemicals i.e., alkaloids, flavonoids, saponins, diterpenes, phytosterol, and phenols. Total phenolic and flavonoid compounds were also observed. The efficacy of A. indica was highest, characterized by the lowest infestation rate (16.65%), host seed weight loss (7.85%), mean oviposition (84.54), and adult emergence (58.40%). In contrast, T. orientalis was found to be the least effective against C. maculatus, with the highest infestation rate of 25.60%, host seed weight loss of 26.73%, mean oviposition of 117.17, and adult emergence rate of 82.01%. Probit analysis was performed by estimating LC₅₀ and LC₉₀. The toxicity percentages of N. tabacum (LC₅₀ = 0.69%, LC₉₀ = 14.59%), N. rustica (LC₅₀ = 0.98%, LC₉₀ = 22.06%), and A. indica (LC₅₀ = 1.09%, LC₉₀ = 68.52%) were notable in terms of the lower LC₅₀ and LC₉₀ values after the 96-h exposure period against C. maculatus. Repellency was assessed by using the area preference and filter paper method. The repellency of C. maculatus on plant extracts increased with the increasing dose and time, such that it was the highest after 48 h. Likewise, at a 3% concentration, A. indica demonstrated 100.00% (Class-V) repellency followed by N. tabacum (96.00%, Class-V), N. rustica (74%, Class-IV), M. azedarach (70.00%, Class-IV), and T. orientalis (68.00%, Class-IV). Based on the findings of this study, we recommend integrating N. rustica, N. tabacum, A. indica, and M. azedarach for effective management of C. maculatus and highlight the potential of these plant species in the formulation of new biocidal agents. Full article