Search Results (115)

Koshu wine, produced from the indigenous Japanese grape Vitis vinifera L. cv. Koshu exhibits a lower pH than other white wines, hindering malolactic fermentation (MLF) by lactic acid bacteria (LAB). Here, we aimed to isolate LAB strains capable of performing MLF under these challenging conditions to improve wine quality. Sixty-four Oenococcus oeni and one Lactobacillus hilgardii strain were isolated from Koshu grapes and wines that had undergone spontaneous MLF. MLF activity was assessed under varying pH, SO₂, and ethanol conditions in modified basal medium (BM) and Koshu model wine media. Expression of stress-related genes was analyzed using real-time PCR. Carbon source utilization was evaluated via API 50CH assays. All isolates degraded malic acid and produced lactic acid at 15 °C and pH 3.2 in BM without reducing sugars. Seven strains, all identified as O. oeni, demonstrated MLF activity at pH 3.0 in modified BM lacking added reducing sugars or tomato juice. Six wine-derived strains tolerated up to 12% ethanol, whereas the grape-derived strain was inhibited at 10%. In a synthetic Koshu wine model (13% ethanol, pH 3.0), wine-derived isolates exhibited higher MLF activity than commercial starter strains. In high-performing strains, mleA was upregulated, and most isolates preferred fructose, arabinose, and ribose over glucose. These findings suggest that indigenous O. oeni strains from Koshu wine possess unique stress tolerance and metabolic traits, making them promising candidates for region-specific MLF starter cultures that could enhance Koshu wine quality and terroir expression. Full article

Thermovinification has emerged as a rising alternative method in red wine production, gaining popularity among winemakers. The use of autochthonous yeasts isolated from grapes is also an interesting practice that contributes to the creation of wine with a distinctive regional character. This research investigated how combining thermovinification with autochthonous yeast strains influences the fermentation dynamics of Syrah wine. Six treatments were conducted, combining the use of commercial and two autochthonous yeasts with traditional vinification (7-day maceration) and thermovinification (65 °C for 2 h) processes. Sugars and alcohols were quantified during alcoholic fermentation by high-performance liquid chromatography with refractive index detection. Cell viability and kinetic parameters, such as ethanol formation rate and sugar consumption, were also evaluated. The Syrah wine’s composition was characterized by classical wine analyses (OIV procedures). The results showed that cell viability was unaffected by thermovinification. Thermovinification associated with autochthonous yeasts improved the efficiency of alcoholic fermentation. Thermovinified wines also yielded a higher alcohol content (13.9%). Future studies should investigate how thermovinification associated with autochthonous yeasts affects the metabolomic and flavoromic properties of Syrah wine and product acceptability. Full article

Winemaking involves a microbial ecosystem where yeast diversity, shaped by terroir and winemaking conditions, determines wine characteristics. Understanding the microbial diversity of vineyards and spontaneous fermentation is crucial for explaining a winery’s typical wine profile. Studying and inoculating indigenous strains make it possible to produce high quality wines, reflecting the production environment. This study analyzes the yeast species involved in 16 spontaneous fermentations (8 in 2022 and 8 in 2023) from grapes of four distinct vineyards under two sets of winemaking conditions. A total of 1100 yeast colonies were identified by MALDI-TOF and DNA sequencing techniques. Saccharomyces (S.) cerevisiae and Hanseniaspora uvarum were the most prevalent species, alongside significant populations of non-Saccharomyces yeasts such as Lachancea thermotolerans and Metchnikowia pulcherrima, which were the most abundant ones. Minor yeast species, including Aureobasidium pullulans, Starmerella bacillaris, Kazachstania servazzi, and other Hanseniaspora spp., were also detected. The results demonstrated that yeast diversity in spontaneous fermentations varied according to vineyard origin and winemaking conditions. Differences between the two vintages studied indicated that annual climatic conditions significantly influenced yeast diversity, especially among non-Saccharomyces species. This substantial diversity represents a valuable source of indigenous yeasts for preserving the typicity of a winery’s wines under controlled conditions. Full article

This study investigated young white wines produced during the 2021 and 2022 vintages from Pedro Ximénez grapes cultivated in three different terroirs within a high-quality production zone. The general oenological parameters were significantly influenced by vintage and terroir (p ≤ 0.001), with ethanol and reducing sugars specifically affected by the terroir and its interaction with the vintage. Multivariate analysis of major and minor volatile compounds enabled the characterization of terroir-specific volatile profiles. However, principal component analysis (PCA) grouped samples by vintage rather than terroir. Ethyl esters of medium- and long-chain fatty acids and certain acetates of higher alcohols were the most discriminant volatiles and were proposed as key compounds for differentiating wines by terroir and vintage. These findings underscore the influence of the terroir on the volatilome and support its relevance in defining wine typicity and quality. Full article

This study aims to identify and evaluate the essential design features, strengths, and limitations of a virtual reality (VR) application that has been developed to train an international sales force effectively for a premium global wine brand. The study emphasizes the value of stakeholder-driven iterative development and systematic evaluations. A case study methodology was adopted for the research, focusing on a VR training application, developed for Marchesi Antinori. The Scrum framework was employed to facilitate iterative stakeholder collaboration. A qualitative evaluation was conducted using focus groups, comprising marketing, communications, and sales representatives. A systematic application of natural language processing (NLP) embedding techniques and recursive clustering analyses was undertaken to interpret stakeholder feedback. The findings suggest that stakeholder-driven, iterative processes can significantly enhance the effectiveness of VR applications by providing a clear structure for immersive storytelling that focuses on terroir characteristics, vineyard operations, and cellar practices. Stakeholders acknowledged the potent educational benefits of VR in regard to business-to-business (B2B) sales training. However, they also highlighted significant limitations, including user discomfort, concerns about authenticity, and variations in market receptivity. Alternative immersive technologies, including augmented reality and immersive multimedia environments, have emerged as valuable complementary approaches. This study addresses a significant gap in the literature by examining the application of VR technology for B2B sales training in the premium wine industry. The study integrates an iterative Scrum methodology with advanced natural language processing (NLP) analytical techniques to derive nuanced, context-rich insights. Full article

Malolactic fermentation conducted by lactic acid bacteria is essential for enhancing wine’s sensory qualities. Although this process can occur spontaneously through the action of native lactic acid bacteria (LAB) from the grapes and cellar, it carries risks such as increased volatile acidity, consumption of residual sugars, and the formation of undesirable metabolites like biogenic amines. This study evaluated the oenological potential of three innovative native malolactic fermentation starters (MLFS) on a pilot scale, aiming to offer local wineries cost-effective MLFS with native LAB strains. Alcoholic fermentation of Malbec grapes was carried out using a commercial yeast starter, followed by a sequential inoculation of native malolactic starters formulated with (i) mesophilic Lactiplantibacillus plantarum UNQLp11 and Oenococcus oeni UNQOe73.2, both from the Province of Río Negro; (ii) psychrotrophic Lentilactibacillus hilgardii UNQLh1.1 and Oenococcus oeni UNQOe19, both from the Province of Río Negro; (iii) mesophilic Lactiplantibacillus plantarum UNQLp1001, from the Province of Buenos Aires; and (iv) a commercial malolactic started, which served as a control. Malic acid was consumed by all starters. RAPD-PCR using M13 primer showed the native LAB’s capability for implantation in wine. A sensory analysis revealed distinct profiles for each formulation, despite having been inoculated at the end of the same alcoholic fermentation. These MLFS could replace imports, enhancing the region’s unique terroir. Full article

Latin America offers a unique point of view into the adaptation of viticulture to climate change through its rich diversity of climates, traditional knowledge, and scientific innovation. This review synthesizes the current research and technological developments across major wine-producing countries including Argentina, Brazil, Chile, Uruguay, the Dominican Republic, and Haiti. Argentina shows key adaptation strategies, including high-altitude vineyard relocation, clonal and rootstock selection, canopy and water management, and the conservation of Criolla and other autochthonous grapevine varieties. In Brazil, tropical viticulture and breeding programs led by Embrapa exemplify advancements in disease-resistant and climate-resilient cultivars. Chile’s heroic and southern viticulture highlights the importance of old vines, microclimatic heterogeneity, and territorial identity. Uruguay stands out for its terroir-based research and producer-led adaptation strategies. This review also addresses systemic challenges in scientific publishing, particularly the underrepresentation of Latin American researchers in global vitivinicultural discourse. These disparities underscore the need for inclusive science that values local knowledge and promotes equity in research funding and dissemination. Overall, Latin America stands out not only as a region highly vulnerable to climate change, but as an emerging model of adaptation and innovation, demonstrating how resilient, sustainable, and culturally rooted wine production can thrive under shifting environmental conditions. Full article

The soil microbiome is a complex system that encompasses millions of microbes including archaea, bacteria, fungi, protozoa and viruses. The role of abiotic factors is crucial in shaping the distribution patterns of microorganisms, its abundance and also the interactions between species, from local to the global level. In the particular case of the vineyard, the microbial communities have a potential impact in both the grapevine development and health and, later on, in the grape production and quality. The present review focuses on how the composition of soil microbial communities is influenced by climate and several edaphic factors, such as soil moisture, soil nutrients and soil pH. It also discusses the role of microorganisms and their metabolic activity on the fermentation process, influencing the sensorial characterisation of the wine and suggesting the definition of a microbial terroir. Full article

This study evaluates the influence of terroir on the physico-chemical characteristics and aromatic profiles of Fetească albă wines from five major Romanian wine regions. By analysing key factors such as soil types, climatic conditions, and winemaking techniques, the study aims to understand their impact on wine composition and quality. The study includes the analysis of superior alcohols, fatty acids, esters, carbonyl compounds, terpenes, and 4-mercapto-4-methylpentan-2-one, which are essential in defining the aromatic complexity of the wines. The results show how fermentation conditions, temperature control, microbial activity, and oxidative exposure contribute to the evolution of these compounds, influencing the sensory profiles. Significant differences in compound concentrations were observed across the regions, with wines from Dealu Mare and Murfatlar exhibiting high ester content and aromatic complexity, while those from Dealul Bujorului and Panciu showed elevated volatile acids and aromatic alcohols, suggesting distinct fermentation dynamics. Climatic factors, such as temperature and precipitation, played a crucial role in shaping the volatile composition, with elevated temperatures in 2021 enhancing ester formation and higher alcohol production. This study demonstrates the critical role of terroir and winemaking practices in shaping the aromatic and sensory characteristics of Romanian Fetească albă wines. Full article

Ten vineyards, belonging to five wine regions, were selected in this study. Maturity indicators and phenolic and aromatic compounds were measured to investigate relationships with factors related to the terroir, including climate conditions and soil nutrients. Multiple factor analysis of all compositions showed that different wine regions or vineyards had different characteristics, which were partly associated with the terroir. The results showed that Shizuishan had high sugar content. A high level of anthocyanins could be found in Qingtongxia and Yongning, respectively. Moreover, Qingtongxia had higher concentrations of monomeric phenols in grapes than the others. LSYH (Lanshanyunhao) and LL (Lilan) had higher YAN (yeast assimilable nitrogen) content and pH in grapes, but their wine had a green flavor (high concentration of alcoholic volatile compounds). The Shizuishan and Hongsipu wine regions had fruitier flavors (high concentration of ester volatile compounds). This study demonstrated the characteristics of different vineyards and wine regions, providing a direction for the future development of region-specific grapes and wines. Full article

The comparison of equally important prerequisites for viticulture development (climatope and edaphotope) in two historical regions has shown that both in the historical era and the present day, the less favorable conditions of a temperate climate (riskier in terms of frost danger) and poorer biogeochemical properties of soils and rocks in the steppe region compared to the conditions of the Sub-Mediterranean region have had a decisive influence on the volume and quality of wine products. This is supported by historical data on the local consumption of produced wine and imports of higher-quality wine from Sub-Mediterranean vineyards (Tauric Chersonesos). Both regions of Western Crimea are characterized by the active bioaccumulation of iron, copper, and manganese in the humus horizon of soils, which creates a specific regional geochemical background with the potential to approach the ecological threshold of danger during long-term viticulture. The specific features of modern agricultural technology in viticulture lead to high concentrations of elements such as Cu, V, Cr, and Ni, which can exceed the MPC levels 20–25 years after the vineyard is planted, earlier than other elements. This makes it possible to identify this list of heavy metals as a priority in agroecological monitoring systems for vineyard lands and set the task of adjusting current agricultural technologies for sustainable viticultural practices in specific soil and climatic conditions. The authors see prospects for further studies both on improving methods for assessing components of a wine terroir, especially biogeochemical indicators of soils for a specific geographic region, as well as improving technology for obtaining conjugate assessments of viticulture products and wine materials connected with a given edaphotope. Full article

Malolactic fermentation (MLF), an essential enological process for wine deacidification and aroma development, is predominantly mediated by Oenococcus oeni (O. oeni). This investigation characterized 170 indigenous O. oeni isolates from two principal Chinese viticultural regions (Yinchuan, Ningxia, and Changli, Hebei) through polyphasic analysis. Forty-nine strains demonstrating genetic potential for efficient malate metabolism and biosafety compliance (absence of ethyl carbamate and biogenic amines genes) were subjected to adaptive laboratory evolution under enologically relevant stress conditions. Comparative evaluation with the superior indigenous strain SD-2a revealed eight stress-adapted isolates exhibiting superior MLF kinetics, completing L-malic acid degradation in Marselan wine. Solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) profiling identified three isolates’ (3-31, 9-10, and 9-50) significant enhancement of key fermentation aromas in experimental fermentations. These oenologically adapted indigenous strains demonstrate promising potential as regional-specific starter cultures, providing a scientific foundation for developing terroir-expressive winemaking practices and optimizing microbial resources in China’s wine industry. Full article

Demonstrating the authenticity and traceability of quality wines based on parameters that reflect their composition and provenance contributes to protecting wine authenticity and to increasing consumer confidence in moderate wine consumption, which is associated with numerous health-promoting properties. A wine’s phenolic fingerprint is increasingly used to assess its authenticity, even though wine phenolic composition is influenced by genetic and environmental factors, as well as vineyard management and enological practices, and storage conditions. This study presents a comprehensive analysis of the bioactive characteristics (total polyphenols—TPs, total flavonoids—TFs, antioxidant activity—AA, and total anthocyanins—TAs) by spectrophotometric analysis and phenolic compound profile (by UHPLC-HRMS analysis) of 19 white and 21 red wines with a Protected Designation of Origin (PDO) from four vineyards located in the wine-growing region of Oltenia, Romania. Multivariate statistical analysis, specifically principal component analysis and heat map analysis, applied to analytical data, enables the discrimination of wines based on grape variety and terroir, and across four consecutive vintages (2019–2022). The phenolic profiles of the wines obtained under standardized winemaking conditions depend on the climatic data specific to each harvest year (temperature, precipitation, duration of sun exposure during grape berry phenological stages, and ripening). The phenolic biomarkers of red wines, such as epicatechin, catechin, gallic, caffeic, t-ferulic acids, t-resveratrol and hesperidin, represent specific biomarkers of warmer and sunnier harvest years with lower precipitation, as observed in the 2021 harvest year. Additionally, our results contribute to the identification of specific phenolic biomarkers for geographical and varietal discrimination, as well as to the promotion of high-quality wines produced in a renowned wine-growing region of Romania. Full article

Besides the other factors, the microclimate (terroir) influences the quality characteristics of wine. The Assyrtiko variety has adapted to the volcanic soil of Santorini but under climate change, finding an ideal location for full grape ripening represents a challenge in preserving the PDO quality of Santorini wines. Thus, this study aims to evaluate the effect of location and harvesting time on the quality of Assyrtiko wine. It assessed the location effect (three distant plots of land in three distant areas of the island) on the composition of grapes (water uptake, pH, sugar, and organic acid accumulation) during the ripening. The grapes were vinified using the same procedure. The aromatic volatile profile of the wines was evaluated with GC-MS. A two-way ANOVA revealed that besides location and harvest time, their interaction is also significant for the parameters studied, except for the interaction effect involving sugar content. The analysis of volatile aromatic compounds revealed that the wine from grapes harvested at a later date had a higher aromatic intensity with notes of citrus, white-fleshed, and tropical fruits. This wine surpasses the levels of 2-phenylethanol, isoamyl acetate, linalool, and 2-phenylethyl ester with 17.8%, 7.7%, 21.1%, and 15.6%, respectively, compared to the immediate next in descending order. Results suggest that the grape variety is better suited to the local climatic conditions when full grape ripeness is reached by the end of the growing season. Full article

In this research, the differences between two terroirs belonging to the Protected Designation of Origin (PDO) Montilla–Moriles (Spain) were analyzed. Both areas share soil and climate characteristics, grape varieties, viticultural practices, and winemaking processes. Therefore, the objective of this study was to establish differences between both areas based on the microbiome of the must, the oenological parameters, and the majority and minority volatile compounds of the wines, thus determining the identity traits that make the wines from both areas so different. The results obtained are quite revealing, since at the microbiome level qualitative differences were established between the various areas. In the quality area, the predominant species is Torulaspora delbrueckii while in the production area it is Hanseniaspora opuntiae. Regarding the volatilome, it was observed that the aromatic profile of the wines from the production area has more citrus-fruity aromas and the quality area has honey-floral aromas, thus producing unique wines from each of the areas. Full article