Search Results (1,670)

The growing demand for healthier food options has accelerated the development of innovative fat-replacement strategies in spreadable products. Oleogels are semi-solid systems formed by structuring edible oils. Recently, these systems have emerged as a promising solution for reducing saturated fat content without compromising product quality, texture, or sensory attributes. A systematic review was conducted following the PRISMA 2020 protocol, supplemented by a bibliometric analysis. Research was identified through searches in Web of Science, Scopus, Wiley, Springer, MDPI, and Google Scholar for studies published between 2020 and 2024. Inclusion criteria focused on original research articles in English involving food-sector applications of oleogels and aerogels in sweet spreads. Study quality and risk of bias were assessed by two independent reviewers based on methodological relevance and data integrity. Results were synthesized through a narrative approach and bibliometric mapping. After screening 490 records, 34 original research articles were included. Bibliometric data highlighted a clear trend shifting from foundational lipid structuring research in 2020 toward complex, product-specific functional applications by 2024. Overall, the results suggest that these structured systems are viable replacements for traditional saturated fats, providing comparable spreadability and stability. Funding: This work was supported by the Croatian Science Foundation under the project IP-2022-10-1960. This systematic review was not registered in a public database. Full article

This study assessed consumer perceptions of high-fat, low-carbohydrate (HFLC) protein bars containing varying levels of beef tallow fat. A consumer acceptability test was conducted (n = 102) with four prepared and one commercially available HFLC bar samples. Hedonic, diagnostic (intensity), and just-about-right (JAR) questions on overall liking, texture, flavor, and purchase intent were included in the sample evaluation ballot, followed by general demographic, consumption behavior, and ingredient preference questions about the product category. Although none of the samples, including the commercial bar, were liked, the sample with the highest protein content and lowest fat content was preferred over the others. Overall flavor and aroma liking were rated significantly higher for all prepared samples compared with the commercial bar (p ≤ 0.05). The sample evaluation revealed potential pathways for improving HFLC bars by leveraging “fat-synergizing” attributes such as sweetness, saltiness, and spiciness, with texture improvements possible through higher lean-protein incorporation. The ingredient factors most important to the participants were high protein content, high satiety, minimal ingredients, natural ingredients, and no added sugar. This study’s results demonstrate a widespread desire for fewer ingredients overall, more natural ingredients, and high satiation in snack products. Full article

Efficient resource allocation during fruit expansion and ripening is critical for enhancing mango (Mangifera indica L.) productivity and fruit quality. A study was conducted to quantify the effects of foliar-applied GA₃ at concentrations of 0 (control), 50 (GA50), 100 (GA100) and 200 (GA200) mg L⁻¹, applied at 15, 25 and 35 days after full bloom, on fruit physiochemical attributes during the fruit expansion and ripening phases. In addition, metabolic profiling and pathway analysis were conducted after fruit ripening. Compared with the control, GA₃ application at 50, 100, and 200 mg L⁻¹ increased fruit length by 8, 12, and 14%, and fruit diameter by 5, 11, and 14%, respectively. The mean single-fruit weight was increased by 5–11% at physiological maturity. During the fruit expansion phase, GA₃ treatment decreased starch and total acidity by up to 11% and 29%, respectively, while increasing the soluble sugar content by 21%. Furthermore, enhanced antioxidant enzyme activities (SOD, POD, and CAT), accompanied by a reduction in malondialdehyde (MDA) contents in leaves, were observed. At the ripening stage, GA₃-treated fruits exhibited lower weight loss, higher firmness, more uniform color development, and reduced disease incidence, although vitamin C content and total soluble solids declined. PCA analysis identified GA100 as the optimal treatment. Metabolomics analysis revealed 287 differentially regulated metabolites between GA100 and the control. Sweet, fruity, and floral compounds were upregulated, whereas terpenoids and aldehydes were downregulated. KEGG pathway analysis indicated that GA100 modulated key resource-related metabolic pathways, including nitrogen, carbon and energy metabolism, thereby promoting efficient resource allocation toward fruit growth, quality, and aroma development. Overall, preharvest foliar application of GA₃, particularly at a concentration of 100 mg L⁻¹ (GA100), markedly improved mango fruit growth and quality but tended to simplify the aroma profiles by favoring ester production over complex terpenoid-derived notes. Full article

Traditional Chinese sauerkraut production is hindered by prolonged fermentation times, inconsistent quality, and nitrite accumulation during spontaneous fermentation, which collectively hinder industrial scalability. Levilactobacillus brevis JYX2, isolated from traditional fermented sauerkraut, exhibits excellent acid/salt tolerance, alongside distinctive metabolic pathways (for example, heterolactic fermentation, nitrite degradation, etc.), presenting a viable solution to these challenges. This study assessed its ability to improve sauerkraut quality at laboratory and pilot scales. Results indicated that JYX2 inoculation significantly reduced fermentation duration, expedited pH decline, and elevated total acid levels compared to spontaneous fermentation. The nitrite concentration in the inoculated sauerkraut was 0.72 mg/kg (p < 0.05), significantly lower than 1.86 mg/kg in spontaneous group. At pilot scale, nitrite levels further decreased to 0.44–0.70 mg/kg (p < 0.05), which is below the spontaneous group’s level of 1.61 mg/kg and approaches the “not detected” threshold (<1 mg/kg). Additionally, inoculation increased total organic acids, particularly lactic acid, and enhanced umami/sweet amino acid proportions. Microbial community analysis showed that JYX2-inoculated groups maintained higher relative abundances of core genera, including Leuconostoc and Latilactobacillus, with Firmicutes as the predominant phylum. Pilot tests confirmed consistent JYX2 performance during scale-up, with uniform quality across sauerkraut layers, showing strong industrial adaptability. In conclusion, JYX2 inoculation shortens fermentation, reduces nitrite levels, enhances flavor, and supports standardized, safe, efficient fermented vegetable production. Full article

Obtaining enzymes through bioconversion depends on a complex relationship between the microorganisms and the biomass used. Here, we evaluate xylanase production by diverse actinobacterial species, cultivated using xylan as the sole carbon source and complex media containing sorghum as the substrate. Fifty-three actinobacteria were tested for xylanase production in a solid medium. Seventeen strains produced xylanase and were tested for their ability to produce xylanase, total cellulases (filter paper activity, FPase), and endoglycanase in submerged culture using a defined liquid medium. The best xylanase-producing species was Streptomyces capoamus, yielding 24 IU·mL⁻¹. For FPase, Streptomyces sp. showed the highest yield (1.12 IU·mL⁻¹); for endoglycanase, the best producer was Streptomyces ossamyceticus (0.99 IU·mL⁻¹). When sweet sorghum was used alone, S. curacoi, S. ossamyceticus, and S. capoamus showed xylanase activities of 4.5 IU·mL⁻¹, 4.4 IU·mL⁻¹, and 0.8 IU·mL⁻¹, respectively. However, FPase activity was not detected under the assay conditions. The results showed that there is an intraspecific difference in xylanase, endoglucanase, and FPase production by actinobacteria, with the species S. curacoi, S. ossamyceticus, and S. capoamus able to use sorghum as a carbon source, demonstrating biotechnological potential. Full article

Sweet potatoes (Ipomoea batatas (L.) Lam.) are known for their anti-inflammatory effects, which are attributed to their phytochemical content. Our previous study revealed that ethanol extracts of sweet potato storage roots (SP-EtOH-Ex) inhibit interleukin-6 (IL-6) production in RAW264.7 cells stimulated with lipopolysaccharide (LPS). However, the causative compounds responsible for the anti-inflammatory effect have not yet been identified. This study aims to identify the compounds responsible for the anti-inflammatory effect of SP-EtOH-Ex using liquid chromatography–quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). The unknown compounds were measured using the auto MS/MS mode (data-dependent acquisition; DDA) of LC-Q-TOF-MS, and the resulting data were analyzed using MS-DIAL and MS-FINDER and also compared with those of the corresponding reference standards in terms of retention time and fragment ions. As a result, β-sitosterol (2.527–4.850 µg/mL), campesterol (75.74–93.63 ng/mL), and lauroyl diethanolamide (4.568–9.260 ng/mL) were identified and quantified in SP-EtOH-Ex. Moreover, the anti-inflammatory effect of these three compounds against RAW264.7 cells was investigated at varying concentrations of β-sitosterol (1 µg/mL, 5 µg/mL, 10 µg/mL), campesterol (10 ng/mL, 100 ng/mL, 1000 ng/mL), and lauroyl diethanolamide (1 ng/mL, 10 ng/mL, 100 ng/mL). The phytosterols β-sitosterol and campesterol suppressed LPS-induced IL-6 production at concentrations comparable to those present in SP-EtOH-Ex. In contrast, lauroyl diethanolamide did not similarly suppress LPS-induced IL-6 production. These results suggest that β-sitosterol and campesterol in sweet potato storage roots contribute to their anti-inflammatory effects. The lack of activity in lauroyl diethanolamide further supports that phytosterols are the primary anti-inflammatory constituents. The edible portion of sweet potatoes holds promise as a promising raw material with anti-inflammatory properties. Full article

Climate change is increasingly exposing sweet chestnut (Castanea sativa Mill.) to more frequent and prolonged drought events, which can compromise growth and nut production, particularly in Mediterranean environments. Understanding how trees respond physiologically to ecological and environmental constraints requires a detailed analysis of their architectures. The aim of this study was to investigate how the shoot vigour and leaf water status of mature chestnut trees vary with height within the canopy. Three mature chestnut trees with distinct crown architectures were selected in a traditional chestnut orchard in Central Italy; the differences in crown structure reflected individual tree development under comparable pruning practices. Morphological traits, leaf water status, and physiological parameters related to chlorophyll were measured directly within the canopy by professional tree climbers, allowing access to both lower and upper shoots during the growing season of 2020. One tree, called “Tree 1,” characterised by low bifurcation, with all epicormic shoot cluster (complexes) located on the two main branches and none on the main stem, showed partial vertical differences, mainly in water status and chlorophyll traits. “Tree 2”, characterised by high bifurcation and shoots running along the main stem, exhibited clear vertical gradients: lower-canopy shoots had larger leaf areas and more dry mass, higher relative water content, and better photosynthetic performance index e values than upper shoots. At the end, “Tree 3”, with the same architecture as Tree 1, displayed no consistent vertical trends. These findings indicate that individual tree architecture modulates hydraulic constraints and shoot vigour, even in hydraulically efficient epicormic branches. Although canopy access constraints limited the number of trees and measurements, this study—among the few to conduct in-canopy measurements on large, mature trees—provides valuable guidance for pruning and crown management, suggesting that lowering and simplifying the crown can enhance water-use efficiency, shoot vigour, and drought resilience in traditional and low-input chestnut orchards. Full article

Carrot juice processing generates large amounts of pomace, a fibre-rich by-product with significant valorisation potential. This study explored the feasibility of fermenting carrot by-product with Levilactobacillus brevis AM7 and Leuconostoc pseudomesenteroides DSM20193 to produce exopolysaccharide (EPS)-enriched functional beverages. Beverages were fermented with or without sucrose addition (EPS⁺ and EPS⁻, respectively) and characterized for microbiological, biochemical, rheological, and sensory attributes. Both strains showed robust growth (>8 log cfu/mL) and acidification (final pH below 4.8), comparable to plant-based yoghurt alternatives, with EPS synthesis markedly enhanced in sucrose-supplemented beverages. Leuc. pseudomesenteroides DSM20193 synthesized the highest EPS concentration (16.8 g/100 g dry weight), resulting in a 6-fold viscosity increase compared to EPS⁻ samples, thus improving the adherence to the spoon and preventing syneresis of the beverages. Sensory evaluation revealed that EPS⁺ carrot-based beverages had improved sweetness due to a slight sucrose residue, aroma, and mouthfeel, while maintaining low off-flavours and high colour uniformity. The results highlight carrot by-product as a promising substrate for developing clean-label beverages that are rich in dietary fibres and polyphenols and show antioxidant and potential prebiotic properties through sustainable fermentation processes. Full article

Sweet corn (Zea mays var. saccharata) is a widely cultivated crop valued for its sweet flavor and high nutritional content. Over the past decade, the area devoted to sweet corn grain production has increased substantially, driven by both its nutritional qualities and its economic value. In this context, we aimed to evaluate the impact of three genotypes (Royalty F₁, Hardy F₁ and Deliciosul de Bacau,) under two fertilization types (chemical and organic) compared with a control version on yield, biometrical, biochemical, and quality parameters. This research was carried out between 2022 and 2023 at an experimental station situated in the North-East region of Romania. The results revealed significant influences of cultivar, fertilization method, and the interaction between these two experimental factors on most of the analyzed indicators. Regardless of the fertilization type, the genotype Hardy F₁ showed higher levels of photosynthetic activity, polyphenols (2.22 mg/g d.w.) and sucrose (6.7 g/100 g d.w.), leading to greater yield (13,995 kg/ha) than that of Deliciosul de Bacau and Royalty F₁. Research on fertilization has shown that sweet corn grains under an organic method have higher levels of lycopene, chlorophyll a, chlorophyll b, total phenolic content (TPC), and fructose. In contrast, chemical fertilization more effectively supported growth, photosynthetic activity, yield, and the content of antioxidants and tannins. Regarding the combined influence of these factors, most of the nutritional characteristics of Royalty F1 were enhanced by organic fertilization, whereas those of the Hardy F1 genotype were improved by chemical fertilization. These findings provide practical guidance for selecting appropriate genotype–fertilization combinations to optimize the yield and nutritional quality of sweet corn and highlight key priorities for further research on sustainable fertilization strategies under climate change conditions. Full article

Metal pollution from metallurgical emissions poses serious environmental and public health risks in Kazakhstan. A replicated pot-culture experiment (n = 4) in a completely randomized design under controlled phytotron conditions evaluated biomass production and metal accumulation in six crop and forage species, alfalfa (Medicago sativa), amaranth (Amaranthus spp.), corn (Zea mays), mustard (Brassica juncea), rapeseed (Brassica napus), and sunflower (Helianthus annuus); three ornamental species, purple coneflower (Echinacea purpurea), marigold (Tagetes spp., ‘Tiger Eyes’), and sweet alyssum (Lobularia maritima); and three native wild plants, greater burdock (Arctium lappa), horse sorrel (Rumex confertus), and mug wort (Artemisia vulgaris). Plants were grown in soils collected from the Qarmet industrial zone in Temirtau, central Kazakhstan. Initial soil analysis revealed substantial mixed-metal contamination, ranked as Mn > Ba > Zn > Sr > Cr > Pb > Cu > Ni > B > Co. Mn reached 1059 mg·kg⁻¹, ~50-fold higher than B (22.7 mg·kg⁻¹). Ba (620 mg·kg⁻¹) exceeded FAO/WHO limits sixfold, Zn (204 mg·kg⁻¹) surpassed the lower threshold, and Pb (41.6 mg·kg⁻¹) approached permissible levels, while Cr, Cu, Ni, Co, and Sr were lower. Biomass production varied markedly among species: corn and sunflower produced the highest shoot biomass (126.8 and 60.9 g·plant⁻¹), whereas horse sorrel had the greatest root biomass (54.4 g·plant⁻¹). Root-to-shoot ratios indicated shoot-oriented growth (>1–8) in most species, except horse sorrel and burdock (<1). Metal accumulation was strongly species-specific. Corn and marigold accumulated Co, Pb, Cr, Mn, Ni, Cu, B, and Ba but showed limited translocation (transfer function, TF < 0.5), whereas sunflower, amaranth, and mug wort exhibited moderate to high translocation (TF > 0.8 to <1) for selected metals. Corn is recommended for high-biomass metal removal, marigold for stabilization, sunflower, horse sorrel, and mug wort for multi-metal extraction, and amaranth and coneflower for targeted Co, Ni, and Cu translocation, supporting sustainable remediation of industrially contaminated soils. Full article

Starches play a crucial role in determining the expansion, texture, and structural development of extruded meat analogs through their gelatinization behavior and interactions with proteins. In this study, corn, pea, tapioca, sweet potato, and potato starches were incorporated into soy protein-based formulations and processed under low-moisture and high-moisture extrusion conditions to investigate starch-dependent physicochemical properties. Amylose/amylopectin composition and starch pasting properties were evaluated, and the resulting extrudates were characterized in terms of expansion behavior, water-related properties, textural attributes, and internal structure. Distinct differences in pasting behavior were observed among starches, with potato starch exhibiting high peak viscosity and pea starch showing strong viscosity development during cooling. These differences were closely associated with extrusion outcomes, influencing expansion ratio and texture formation. In low-moisture extrusion, starches susceptible to thermal and shear degradation showed increased solubilization, whereas in high-moisture extrusion, enhanced starch gelatinization promoted starch–protein interactions and contributed to improved textural integrity and structural alignment. Overall, the results demonstrate that starch type is a key determinant of expansion behavior, texture, and structural organization in extruded meat analogs, highlighting the importance of starch selection and processing conditions for tailoring product quality. Full article

Background: Ultra-processed food (UPF) consumption has increased significantly in Latin America and Spain, impacting both health and environmental sustainability. To our knowledge, this is the first multicenter study to examine the association between UPF consumption and sustainable lifestyle behaviors in Latin America and Spain. Objective: To evaluate the association between UPF consumption and sustainable lifestyle behaviors in Latin America and Spain. Methods: This was an observational, analytical, multicenter, cross-sectional study. A validated, self-administered online questionnaire was distributed in 14 countries between March 2023 and January 2024. The survey collected sociodemographic data, UPF intake (classified using the NOVA system), body mass index and sustainable lifestyle behaviors (food, transport, environment). Multivariate linear regression models were applied to assess associations, adjusting for age, sex, smoking, physical activity and BMI. Results: Among 6009 adults (mean age: 34.98 ± 12.55; 79.5% women), those with the highest consumption of UPF (fast food, beverages and juices, salty snacks and sweet snacks) were significantly more likely to be in the least sustainable quartile compared to those who did not consume these food products ((OR = 2.51; 95% CI: 1.79–3.54), (OR = 1.82; 95% CI: 1.50–2.22), (OR = 1.51; 95% CI: 1.32–1.73) and (OR = 1.42; 95% CI: 1.20–1.67), respectively, with p values < 0.001). Conclusions: High consumption of ultra-processed foods (UPFs) is inversely associated with sustainable lifestyles. These findings position UPF consumption not only as a health problem but also as a key indicator of unsustainable lifestyles. Full article

Packaged discretionary foods that are energy-dense and nutrient-poor are widely available in the current food environment, potentially contributing to overconsumption and excessive energy intake over time. Factors such as on-pack visual cues (for example, front-of-pack image and food units per serving) and structural features (for example, package transparency) have an important role in nudging consumers towards better portion control. As little is known regarding the presence of these features on packaged discretionary foods in the current retail context, this study aimed to examine the presence of such cues on packaged discretionary foods in Australian supermarkets. Six common packaged snacks were selected: ice-cream, chocolate, lollies, sweet biscuits, savoury biscuits and crisps. Data were collected by in-store visits and using retail websites. A total of 1930 products were included; the majority were share packs (n = 1419, 73.5%), followed by multipacks (n = 385, 19.9%) and single packs (n = 126, 6.5%). Less than half of the share pack products (47%) had front-of-pack images aligned with the manufacturer-suggested serving sizes on the Nutrition Information Panel. Structural features, including transparency, partitioning and resealability, were less common and identified in less than 30% of packaged snacks. Overall, the findings showed that on-pack visual cues and structural features are not commonly used for portion control in packaged discretionary foods in Australian retail settings. Opportunities exist to improve on-pack cues and guides to support better portion size decisions. Full article

The two-spotted spider mite, Tetranychus urticae Koch, is a major agricultural pest whose control is increasingly constrained by resistance to synthetic acaricides. This study evaluated the long-term field efficacy of three commercial entomopathogenic fungal (EPF) biopesticides—Velifer^® (Beauveria bassiana), Metab^® (B. bassiana + Metarhizium anisopliae), and Botanigard^® (B. bassiana)—against larval and protonymph stages of T. urticae on two host plants, Italian cypress (Cupressus sempervirens) and sweet orange (Citrus sinensis). Two foliar applications were conducted during the 2023 growing season (25 May and 25 July), and mite populations were monitored for 140 days after the final application. A randomized complete block design was used, and efficacy was calculated using the Henderson–Tilton formula. All EPF treatments significantly reduced mite populations compared with the untreated control throughout the monitoring period. Velifer consistently achieved the highest suppression of larval populations, particularly on C. sinensis, with efficacy comparable to the chemical standard. Botanigard showed more gradual but sustained population reduction over time, whereas Metab exhibited lower but stable efficacy in all cases. Treatment performance was strongly influenced by host plant species and mite developmental stage, with larvae consistently more susceptible than protonymphs. On C. sinensis, Velifer achieved the highest larval suppression (84.6%), comparable to the chemical standard abamectin, while Botanigard and Velifer were most effective on C. sempervirens. Survival analysis confirmed isolate- and host-dependent differences in hazard effects over time. These results demonstrate that EPF-based products can provide sustained, long-term suppression of T. urticae under field conditions, supporting their integration into integrated pest management programs. Full article

In this study, a pectin polysaccharide named DESP was extracted using a deep eutectic solvent (DES) from sweet potato residue (SPR) and the extract was optimized through response surface methodology (RSM). The DESP, based on choline chloride–urea (ChCl-Ur), was characterized for yield, molecular weight (Mw), and monosaccharide composition. Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ¹H-nuclearmagnetic resonance (¹H-NMR), and scanning electron microscopy (SEM) were used to analyze the structure. Optimal extraction conditions for DESP were ChCl-Ur in a molar ratio of 1:2, water content of 75 wt.%, extraction time of 125.7 min, extraction temperature of 83.2 °C, and a liquid-to-solid ratio of 37.0 mL·g⁻¹. The optimized extraction yield was 5.6% ± 0.09%, which was 2.4 times higher than that of hot-water-extracted sweet potato pectin (HWSP, 2.32%). The monosaccharide analysis revealed that galacturonic acid (GalA) was the most abundant saccharide, followed by glucose (Glc), galactose (Gal), arabinose (Ara), and rhamnose (Rha). The Mw of DESP was 20.90 kDa, which was lower than that of HWSP and HASP. In addition, DESP exhibited certain anti-inflammatory activity. Full article