Search Results (5,037)

Monkeypox virus (MPXV) is a zoonotic Orthopoxvirus responsible for Monkeypox (Mpox), historically associated with sporadic zoonotic transmission but increasingly characterised by sustained human-to-human spread. While vaccinia-based vaccination is known to confer cross-protection against MPXV, the durability of such immunity over a human lifetime remains incompletely characterised. Here, we assessed humoral and cellular immune responses to MPXV in octogenarians and nonagenarians vaccinated against smallpox during childhood. Twenty-three adults aged 79–94 years (median 83), who self-reported childhood vaccinia vaccination between 1925 and 1940, were recruited. MPXV-specific antibody responses were evaluated using ELISA, targeting homologous vaccinia and MPXV proteins, and live-virus neutralisation assays. Cellular immunity was assessed by IFN-γ ELISpot following stimulation with peptide pools derived from highly conserved vaccinia antigens. Responses were also obtained from younger, recently MVA–BN-vaccinated and unvaccinated control donors. All historically vaccinated participants exhibited MPXV-reactive IgG responses, with antibody binding and neutralisation levels comparable to recently vaccinated individuals. Functional neutralising activity against MPXV was detected in all donors, with ≥50% neutralisation observed in 78% of participants. Antibody concentrations correlated strongly with neutralisation capacity. T-cell responses were detectable in all historically vaccinated donors, most prominently against the major core protein A10L, although reduced magnitudes were observed in participants over 90 years of age. No MPXV-specific humoral or cellular responses were detected in unvaccinated controls. These findings demonstrate that childhood vaccinia vaccination induces durable humoral and cellular immunity against MPXV persisting for over seven decades. Historical smallpox vaccination status may therefore remain a relevant determinant of protection against Mpox. Full article

Metformin is a promising small molecule for dentin regeneration, but an effective local delivery system for pulp applications has been underexplored. This study encapsulated metformin in poly(lactic-co-glycolic acid) (PLGA) microspheres and incorporated them into calcium phosphate–chitosan cement (CPCC) as a direct pulp-capping material (DPC). Metformin-PLGA microspheres were prepared by double emulsion and mixed with CPCC at a concentration of 0% to 20% by weight. Microsphere morphology, encapsulation efficiency, chemical composition, and physico-mechanical properties were characterized, and compatibility with human dental pulp stem cells (hDPSCs) was evaluated by live/dead assay and SEM. The microspheres were spherical (5.43 ± 0.17 µm) with (51 ± 3.69%) encapsulation efficiency, and FTIR confirmed metformin incorporation. The 15% Met-PLGA-CPCC group showed flexural strength (15.22 ± 1.98 MPa), elastic modulus (4.60 ± 0.73 GPa), and work of fracture (104.96 ± 12.48 J/m²) comparable to or higher than CPCC and MTA, while all Met-PLGA-CPCC groups had shorter setting times ranging from 18 min to 27 min than CPCC (39.15 ± 2.10 min) and MTA (123 ± 4.2 min). Metformin release increased proportionally with Met-PLGA content. hDPSCs exhibited good attachment and high viability on all materials over the evaluated period. In conclusion, Met-PLGA-CPCC provides fast-setting and favorable physico-mechanical properties, sustained metformin delivery, and excellent hDPSC compatibility. These properties support its potential as a bioactive direct pulp-capping material and as a versatile platform for regenerative applications. Full article

Agricultural expansion is a major driver of biodiversity loss in tropical regions, yet human-dominated landscapes also hold potential for biodiversity conservation when managed as multifunctional mosaics that retain patches of native vegetation. We assessed how natural and semi-natural habitats contribute to avian diversity in a tropical livestock farm in northern Costa Rica. Over one year, bird assemblages were sampled across three habitat types (live fences, pastures and riparian forest) at La Balsa farm. Using point counts surveyed every month during the year, we recorded 165 bird species, including 20 migratory and 6 species of global conservation concern, and 4 regionally endemic species. Species richness and overall abundance were lower in the riparian forest compared to live fences and pastures, and bird assemblage composition differed markedly among habitats, with the community in the riparian forest exhibiting a distinct assemblage structure. These results indicate that though the riparian forest hosts fewer species and individuals, it harbors a characteristic bird assemblage, highlighting its irreplaceable ecological role in providing habitat to forest-dependent species. Overall, the findings underscore that structurally diverse agricultural mosaics can sustain remarkably high bird diversity when complemented by habitats including native vegetation. Full article

Wildfires are increasingly frequent and intense due to climate change, resulting in degraded soils with diminished microbial activity, reduced water retention, and low nutrient availability. In many regions, previously restored areas face repeated burning events, which further exhaust soil fertility and limit the potential for natural regeneration. Traditional reforestation approaches such as seed scattering or planting seedlings often fail in these conditions due to extreme aridity, erosion, and lack of biological support. To address this multifaceted problem, this study proposes a living, biodegradable hydrogel that integrates an engineered soil-beneficial microorganism consortium, designed to deliver beneficial compounds and nutrients combined with endemic plant seeds into a single biopolymeric matrix. Acting simultaneously as a biofertilizer, soil conditioner, and reforestation aid, this 3-in-1 system provides a microenvironment that retains moisture, supports microbial diversity restoration, and facilitates plant germination even in nutrient-poor, arid soils. The concept is rooted in circular economy principles, utilizing polysaccharides from food industry by-products for biopolymer formation, thereby ensuring environmental compatibility and minimizing waste. The encapsulated microorganisms, a Bacillus subtilis strain and a Nostoc oryzae strain, are intended to enrich the soil with useful compounds. They are engineered based on synthetic biology principles to incorporate specific genetic modules. The B. subtilis strain is engineered to break down large polyphenolic compounds through laccase overexpression, thus increasing soil bioavailable organic matter. The cyanobacterium strain is modified to enhance its nitrogen-fixing capacity, supplying fixed nitrogen directly to the soil. After fulfilling its function, the matrix naturally decomposes, returning organic matter, while the incorporation of a quorum sensing-based kill-switch system is designed to prevent the environmental escape of the engineered microorganisms. This sustainable approach aims to transform post-wildfire landscapes into self-recovering ecosystems, offering a scalable and eco-friendly alternative to conventional restoration methods while advancing the integration of synthetic biology and environmental engineering for climate resilience. Full article

Cardiovascular disease (CVD) is increasingly recognized as a significant comorbidity in people living with HIV (PWH), contributing to increased morbidity and mortality. Epidemiological studies indicate that PWH have a 1.2–2-fold higher risk of myocardial infarction (MI) and other CVD events compared to HIV-negative individuals. While the mechanisms underlying HIV-associated CVD are not fully understood, they are likely to include a combination of cardiovascular-related adverse effects of HIV medications, vascular dysfunction caused by HIV-induced monocyte activation, and cytokine secretion, in addition to existing comorbidities and lifestyle choices. This comprehensive review examines the complex relationship between HIV infection and CVD, highlighting key pathophysiological mechanisms such as chronic immune activation, inflammation, endothelial dysfunction, and the role of antiretroviral therapy (ART) in promoting cardiovascular risk. Alongside conventional risk factors such as smoking, hypertension, and dyslipidemia, HIV-specific elements, especially metabolic abnormalities associated with ART, significantly contribute to the development of CVD. Prevention strategies are crucial, focusing on the early identification and management of cardiovascular risk factors as well as optimizing ART regimens to minimize adverse metabolic effects. Clinical guidelines now recommend routine cardiovascular risk assessment in PWH, emphasizing aggressive management tailored to their unique health profiles. However, challenges exist in fully understanding the cardiovascular outcomes in this population. Future research directions include exploring the role of inflammation-modulating therapies and refining sustainable prevention strategies to mitigate the growing burden of CVD in PWH. Full article

A living organism can be regarded as a dissipative, self-organizing physical system operating far from thermodynamic equilibrium. Such systems can be effectively described within the framework of Markov jump processes subjected to an external driving force that sustains the system away from equilibrium—leading, in the special case of stabilization, to a non-equilibrium steady state (NESS). By combining the Markov formalism with concepts from stochastic thermodynamics, we demonstrate the temporal evolution of entropy in such systems: entropy decreases during growth and development, stabilizes at maturity under NESS conditions, and subsequently increases during aging, death, and decomposition. This characteristic trajectory, which we term the entropy bathtub, highlights the universal thermodynamic structure of living systems. We further show that the system exhibits continuous yet time-dependent positive entropy production, in accordance with fundamental thermodynamic principles. Perturbations of the driving force—whether reversible or irreversible—naturally capture the impact of external stressors, providing a conceptual analogy to pathological processes in biological organisms. Although the model does not introduce fundamentally new elements to the physics of life, it offers a simple tool for exploring entropy-driven mechanisms in living matter. Full article

Rural governance is a key means of promoting sustainable rural development and is gradually evolving toward interdisciplinary research across multiple scales. How should governments at all levels implement precise policy to achieve rural revitalization goals? To reveal the multi-scale characteristics of rural spatial governance, this study proposes theoretical hypotheses and constructs a three-level analytical framework encompassing the municipal, functional area, and township dimensions. Taking Nanning City as a case study, it comprehensively employs global and local spatial autocorrelation methods to quantitatively analyze the spatial differentiation patterns and governance effectiveness across the five dimensions of rural revitalization at different scales. The results indicate that indicators such as ecological livability, industrial prosperity, life affluence, rural civilization, and effective governance all exhibit significant clustering patterns at various scales, with notable differences in the intensity of clustering across these scales. Specifically, the ecological livability indicator exhibits the strongest spatial agglomeration at the municipal level (Moran’s I = 0.578), industrial prosperity and affluent living show the strongest correlations at the functional area level (with average Moran’s I values of 0.281 and 0.414, respectively), while rural civilization and effective governance display the most pronounced clustering at the township level (Moran’s I values of 0.363 and 0.350). The findings provide direct evidence for implementing differentiated and precise rural spatial governance in Nanning City and similar regions, while also contributing to the optimization of cross-level policy resource allocation. Future research should further integrate multidisciplinary perspectives and expand the analysis of multi-stakeholder participation mechanisms. Full article

Sustainable development is a global priority, essential for meeting the social, economic, and environmental needs of future generations. Governments worldwide have undertaken extensive research efforts to identify effective pathways for achieving sustainable development. In 2017, the financial sector contributed 15% of the global GDP. Given the central role of financial systems in both national and global economies, their integration into sustainability initiatives is essential. The United Nations Agenda 2030 for Sustainable Development highlights the importance of financial inclusion in addressing economic disparities, particularly in rural African areas where a significant portion of the population lives below the poverty line. This study examines the potential of Financial Technology (FinTech) to drive sustainable development in Nigeria, a country marked by high financial exclusion, wealth inequality, and poverty. Through textual analysis and semi-structured interviews, this research identifies three critical areas for investment to enhance the adoption of FinTech and promote sustainable growth: financial and educational literacy, an enabling environment with supportive policies, and robust regulatory measures to protect end-users. These initiatives are essential for building confidence and fostering greater adoption of FinTech in Nigeria. Full article

Background: Solid organ transplantation (SOT) is a life-saving treatment for patients with end-stage diseases and/or organ failure. However, access to healthy organs is often limited by challenges in organ preservation. Furthermore, upon transplantation, ischemia–reperfusion injury (IRI) can lead to increased organ rejection or graft failures. The work presented aims to address both challenges using an innovative nanomedicine platform for simultaneous drug and oxygen delivery. In recent studies, resveratrol (RSV), a natural antioxidant, anti-inflammatory, and reactive oxygen species (ROS) scavenging agent, has been reported to protect against IRI by inhibiting ferroptosis. Here, we report the design, development, and scalable manufacturing of the first-in-class dual-function perfluorocarbon-nanoemulsion (PFC-NE) perfusate for simultaneous oxygen and antioxidant delivery, equipped with a near-infrared fluorescence (NIRF) reporter, longitudinal, non-invasive NIRF imaging of perfusate flow through organs/tissues during machine perfusion. Methods: A Quality-by-Design (QbD)-guided optimization was used to formulate a triphasic PFC-NE with 30% w/v perfluorooctyl bromide (PFOB). Drug-free perfluorocarbon nanoemulsions (DF-NEs) and RSV-loaded nanoemulsions (RSV-NEs) were produced at 250–1000 mL scales using M110S, LM20, and M110P microfluidizers. Colloidal attributes, fluorescence stability, drug loading, and RSV release were evaluated using DLS, NIRF imaging, and HPLC, respectively. PFC-NE oxygen loading and release kinetics were evaluated during perfusion through the BMI OrganBank^® machine with the MEDOS HILITE^® oxygenator and by controlled flow of oxygen. The in vitro antioxidant activity of RSV-NE was measured using the oxygen radical scavenging antioxidant capacity (ORAC) assay. The cytotoxicity and ferroptosis inhibition of RSV-NE were evaluated in RAW 264.7 macrophages. Results: PFC-NE batches maintained a consistent droplet size (90–110 nm) and low polydispersity index (<0.3) across all scales, with high reproducibility and >80% PFOB loading. Both DF-NE and RSV-NE maintained colloidal and fluorescence stability under centrifugation, serum exposure at body temperature, filtration, 3-month storage, and oxygenation. Furthermore, RSV-NE showed high drug loading and sustained release (63.37 ± 2.48% at day 5) compared with the rapid release observed in free RSV solution. In perfusion studies, the oxygenation capacity of PFC-NE consistently exceeded that of University of Wisconsin (UW) solution and demonstrated stable, linear gas responsiveness across flow rates and FiO₂ (fraction of inspired oxygen) inputs. RSV-NE displayed strong antioxidant activity and concentration-dependent inhibition of free radicals. RSV-NE maintained higher cell viability and prevented RAS-selective lethal compound 3 (RSL3)-induced ferroptosis in murine macrophages (macrophage cell line RAW 264.7), compared to the free RSV solution. Morphological and functional protection against RSL3-induced ferroptosis was confirmed microscopically. Conclusions: This study establishes a robust and scalable PFC-NE platform integrating antioxidant and oxygen delivery, along with NIRF-based non-invasive live monitoring of organ perfusion during machine-supported preservation. These combined features position PFC-NE as a promising next-generation acellular perfusate for preventing IRI and improving graft viability during ex vivo machine perfusion. Full article

Sustainable development is crucial worldwide. Under the Paris Agreement, countries commit to Nationally Determined Contributions (NDCs) assessed every five years. China, a major contributor to global warming, has made significant efforts to reduce carbon emissions and achieve carbon neutrality, a key strategy for sustainable development. However, there is a lack of adequate attention to embodied emission reduction in rural residential construction, despite a surge in building to improve living standards. This paper evaluated the feasibility of applying a multi-ribbed composite wall structure (MRCWS) in rural China through a village service project. A full-scale shaking table test was conducted to study its seismic performance. Carbon emissions were analyzed using process-based life cycle assessment (P-LCA) and the emission-factor approach (EFA), while costs were estimated using life cycle costing (LCC) and the direct cost method (DCM). These analyses focused on sub-projects and specific structural members to validate the superiority of this prefabricated structure over common brick masonry. MRCWS blocks were prefabricated by mixing wheat straw with aerocrete, utilizing agricultural by-products from local farmlands, thus reducing both construction-related carbon emissions and agricultural waste treatment costs. Results show that this novel precast masonry structure exhibits strong seismic resistance, complying with fortification limitations. Its application can reduce embodied carbon emissions and costs by approximately 6% and 10%, respectively, during materialization phases compared to common brick masonry. This new prefabricated building product has significant potential for reducing carbon emissions and costs in rural housing construction while meeting seismic requirements. The recycling of agricultural waste highlights its adaptability, especially in rural areas. Full article

With the continuous expansion of high-density urban forms, residents’ opportunities for daily contact with natural environments have been increasingly reduced, making the equity of urban green space allocation a critical challenge for sustainable urban development. Existing studies have largely focused on green space quantity or accessibility at single time points, lacking systematic investigations into the spatiotemporal evolution of green space exposure (GSE) and its equity from the perspective of residents’ actual environmental experiences. GSE refers to the integrated level of residents’ contact with urban green spaces during daily activities across multiple dimensions, including visual exposure, physical accessibility, and spatial distribution, emphasizing the relationship between green space provision and lived environmental experience. Based on this framework, this study takes the central urban area of Hangzhou as the study area and integrates multi-temporal remote sensing imagery with large-scale street view data. A deep learning–based approach is developed to identify green space exposure, combined with spatial statistical methods and equity measurement models to systematically analyze the spatiotemporal patterns and evolution of GSE and its equity from 2013 to 2023. The results show that (1) GSE in Hangzhou increased significantly over the study period, with accessibility exhibiting the most pronounced improvement. However, these improvements were mainly concentrated in peripheral areas, while changes in the urban core remained relatively limited, revealing clear spatial heterogeneity. (2) Although overall GSE equity showed a gradual improvement, pronounced mismatches between low exposure and high demand persisted in densely populated areas, particularly in older urban districts and parts of newly developed residential areas. (3) The spatial patterns and evolutionary trajectories of equity varied significantly across different GSE dimensions. Composite inequity characterized by “low visibility–low accessibility” formed stable clusters within the urban core. This study further explores the mechanisms underlying green space exposure inequity from the perspectives of urban renewal patterns, land-use intensity, and population concentration. By constructing a multi-dimensional and temporally explicit analytical framework for assessing GSE equity, this research provides empirical evidence and decision-making references for refined green space management and inclusive, sustainable urban planning in high-density cities. Full article

Family caregivers provide up to 90% of care in Alberta’s communities and play an essential role in sustaining the province’s health and social care systems, yet they remain under-recognized and insufficiently supported. To address this gap, we co-designed the Alberta Family Caregiver Strategy and Action Plan (2024–2025), a provincial framework developed through participatory research and collective impact methods. Guided by principles of co-production, equity, and lived experience, the project engaged over 500 stakeholders, including caregivers, healthcare providers, educators, employers, and policymakers, through Phase 1 interviews (health/community leaders, n = 44; Family and Community Support Services (FCSS), n = 47; navigation experts, n = 9), Phase 2 co-design team consultations, and Phase 3 sector roundtables (n = 52). Using reflexive thematic analysis, we identified four foundational caregiver strategies, Recognition, Partnership, Needs Assessment, and Navigation, and four enabling conditions: Education, Workplace Supports, Policy and Research and Data Infrastructure. These elements were synthesized into an eight-priority Alberta Caregiver Strategy and Action Plan Framework, a practical way to connect validated priorities with coordinated, measurable implementation across settings. Participants emphasized four key enablers essential to making caregiver inclusion more feasible and sustainable: education, workplace supports, policy infrastructure, and research and evaluation. Findings highlight strong cross-sector consensus that caregiver inclusion must be embedded into routine practice, supported by consistent policy, and reinforced through provincial coordination with local adaptation. The Alberta Family Caregiver Strategy provides a practical, evidence-informed plan for transforming fragmented supports into a coherent, caregiver-inclusive ecosystem that strengthens both caregiver well-being and system sustainability. Full article

Background: International policy increasingly recognises the importance of inclusive, community-based support for people living with dementia. Football, as a culturally significant sport, has the potential to reach older adults and communities disproportionately affected by health inequalities. The objectives of this review were to collate evidence on football-based dementia initiatives, including intervention format, delivery approaches, and reported outcomes. Methods: Seven databases (Sportdiscus, MEDLINE, Embase, Scopus, PsycINFO, CINAHL, and Web of Science) were searched for relevant peer-reviewed and grey literature from their inception to June 2025. The PICO framework was used to define eligibility criteria. Eligible studies described community-based football-themed or football-based programmes involving people living with dementia. Data were extracted on participant sample, intervention characteristics, and reported outcomes, and iteratively charted. Results: In total, 11 of the 1059 identified articles were included within this review. Initiatives were often delivered through professional football clubs and charitable foundations, with formats ranging from reminiscence therapy sessions to walking football. Common outcomes for participants included increased sociability, improved mood, enhanced communication, and a strengthened sense of identity and belonging. Some interventions also reported physical benefits, such as improved mobility. Carers highlighted respite opportunities, peer support, and enjoyment from seeing relatives more engaged. Despite positive reports, outcome measurement was inconsistent, and most studies were small-scale or descriptive pilot projects. Conclusions: Football-based dementia initiatives provide meaningful, culturally grounded opportunities for social inclusion and support. Their delivery through community clubs/organisations positions them well to address inequities in dementia care, particularly in areas of disadvantage. However, stronger evaluation methods are required to build a robust evidence base and guide sustainable implementation at scale. Full article

Greece lies within the Mediterranean global biodiversity hotspot and harbors exceptional plant richness and endemism, including numerous medicinal and aromatic plants (MAPs). These taxa underpin long ethnobotanical traditions and contemporary bioeconomy niches (culinary herbs, essential oils, phototherapeutics). The aim of this review is to map (i) the biodiversity knowledge base for Greek MAPs, (ii) recent ethnobotanical evidence, and (iii) sustainability pathways (conservation, cultivation, value chains, and regulation) in a Mediterranean context. The information is presented and analyzed in a critical manner. A total of 115 research studies were systematically reviewed based on the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. Key findings highlight that (i) Greece is a regional plant-diversity hotspot with many MAP endemics in Lamiaceae; (ii) contemporary ethnobotanical knowledge persists and adapts; and (iii) strong sustainability levers exist through Natura 2000 coverage, Good Agricultural and Collection Practice (GACP) and European Medicines Agency (EMA) frameworks, and Protected Designation of Origin (PDO)/United Nations Educational, Scientific and Cultural Organization (UNESCO) recognitions (e.g., Krokos Kozanis saffron, Chios mastic), although threats from climate and land-use change remain significant. In conclusion, Greek MAPs combine high biodiversity value, living ethnobotanical traditions, and tangible bioeconomic opportunities. Their sustainable prospects depend on integrating habitat protection, GACP and FairWild Standard (FairWild)-aligned wild collection and cultivation, domestication of priority endemics, and climate-resilience planning, all supported by traceable value chains and Access and Benefit-Sharing (ABS)/Nagoya Protocol compliance. The review concludes with practical recommendations and a prioritized list of flagship taxa for “conservation through use.” Full article

Given global efforts to promote sustainable energy transitions, this study investigates how the deployment of renewable energy technologies (RETs) relates to multidimensional societal welfare and provides empirical evidence on these linkages in Lithuania. The purpose of the study is to provide an integrated, Lithuania-specific assessment of how economic, social, and environmental determinants associated with RET deployment are related to multiple dimensions of societal welfare. Drawing on scientific literature, an integrated indicator framework is developed that links the economic, social, and environmental determinants of renewable energy technology (RET) deployment to six societal welfare dimensions, as defined by the Lithuanian Quality of Life Index. Using official Lithuanian statistics for 2020–2024, a standardized panel dataset is constructed and Pearson correlation analysis and multiple linear regression are applied using aggregated determinant categories, with model assumptions verified using the Breusch–Pagan and Durbin–Watson tests. Correlation results show very strong positive links between RET intensity indicators and key economic welfare measures (for example, wages, GDP per capita, foreign direct investment, disposable income), with absolute correlation coefficients typically between 0.90 and 0.99 (p < 0.05), and strong negative correlations between air-pollution indicators and GDP, income, FDI, and education (correlation coefficients between −0.96 and −0.90; p < 0.05). The results indicate that RET-related economic determinants have a statistically significant positive effect on the societal welfare dimensions of material living conditions; entrepreneurship/business competitiveness; and public infrastructure, living-environment quality/safety. Social factors also significantly support the societal welfare dimensions of entrepreneurship/business competitiveness and public infrastructure, living-environment quality/safety. In the retained regression models, explanatory power is very high (R² between 0.91 and 0.999), with positive and statistically significant coefficients for the economic determinant (regression coefficients between 0.43 and 0.96; p < 0.05) and negative, statistically significant coefficients for the environmental determinant in the entrepreneurship and public-infrastructure dimensions (regression coefficients between −1.13 and −1.51; p < 0.05). Environmental determinants are associated with lower air pollution but show negative effects on the societal welfare dimensions of entrepreneurship/business competitiveness and public infrastructure, living-environment quality/safety. Overall, the findings suggest that RET deployment is an important correlate of the economic aspects of societal welfare, while environmental and social dimensions display more complex, domain-specific impacts. Full article