Search Results (19,714)

Hyaluronan (HA) receptors are expressed in a wide variety of different tissues and have long been known to support the critical cellular functions of adhesion and motility, in addition to a range of different physiological and pathological processes, including immunity, inflammation and tumour metastasis. In recent years, LYVE-1, an HA receptor largely but not exclusively restricted to the endothelia of lymphatic capillaries, has been shown to mediate the entry of immune cells through lymphatic endothelial junctions by engaging with their surface HA glycocalyx, itself anchored to the immune cell membrane by the closely related receptor CD44. Although similar to CD44 in primary sequence, LYVE-1 is functionally distinct, with a mutually exclusive pattern of tissue expression and a marked dependence on avidity for engagement with the long chains of HA—achieved primarily through receptor clustering. Here, we review key data that have defined the in vitro and in vivo functions of LYVE-1, including recent high-resolution crystal structures that have revealed its unusual and reversible “sliding” mode of interaction with HA, as distinct from the conventional “sticking” interaction in CD44. Lastly, we consider the emerging functions of LYVE-1 in sites beyond the lymphatics, namely tissue-resident macrophages and the specialised blood vessels of certain organs, and its potential as a therapeutic target. Full article

Hierarchical functionalisation of the UiO-66(Zr)-NH₂ metal–organic framework with cysteine, poly(ethylene glycol) (PEG), and the SARS-CoV-2 spike receptor-binding domain (RBD) was developed to enable receptor-specific interaction with the angiotensin-converting enzyme 2 receptor (ACE2) in model cells. Post-synthetic modification using cysteine and heterobifunctional PEG linkers allowed controlled bioconjugation of SpyTag-labelled RBD via SpyTag/SpyCatcher chemistry, while preserving the crystallinity, microporosity, and intrinsic optical properties of the UiO-66(Zr)-NH₂ framework. Comprehensive physicochemical characterisation confirmed successful surface functionalisation, tunable aggregation behaviour, and retention of multimodal optical characteristics. Cellular studies in HEK293T and HeLa cells overexpressing EGFP-tagged ACE2 demonstrated enhanced and selective association and uptake of RBD-functionalised nanoparticles compared with non-targeted analogues. Multimodal fluorescence imaging, fluorescence lifetime imaging microscopy, flow-cytometry, and electron microscopy indicated ACE2-dependent endocytic internalisation, with predominant localisation in endosomal and autophagosomal compartments, while both amine- and cysteine-modified formulations exhibited good biocompatibility. Overall, this study establishes a virus-mimetic, ACE2-targeted UiO-66(Zr)-based nanosystem as a proof-of-concept biointerface platform for receptor-specific cellular delivery and imaging, providing a foundation for future MOF-based nanocarriers exploiting ligand–receptor interactions. Full article

This study investigates whether Yuan Dynasty Jingdezhen Luanbai glaze can support cobalt-blue coloration under conditions relevant to early blue-and-white porcelain production. Comparative analyses of archaeological Luanbai and blue-and-white specimens show that the two glaze types have similar average thicknesses, approximately 0.20 mm, and comparable basic chemical compositions, especially in their SiO₂ and Al₂O₃ contents. These results suggest that they belong to related high-temperature calcium–alkali glaze traditions rather than completely isolated technological systems. Simulated firing experiments using five cobalt pigments of different compositions further indicate that Luanbai glaze can support cobalt-blue coloration at 1300 °C in a reducing atmosphere. Compared with unglazed controls, Luanbai-glazed samples showed a more consistent blue appearance and clearer pigment–glaze interaction. XPS and SEM-EDS line-scan analyses revealed differences in the near-surface chemical environment and cross-sectional distribution of Co, Mn, and Fe between glazed and unglazed samples, supporting the role of glaze coverage in color development. Refiring experiments on authentic Yuan sherds further supported the feasibility of cobalt-blue coloration on historical Luanbai glaze surfaces. Overall, the results suggest that the opalescent appearance of Luanbai glaze is not an inherent barrier to underglaze cobalt decoration. This work provides experimental evidence for reassessing the technological relationship between Luanbai and early blue-and-white porcelain. Full article

Corrosion fatigue is an accelerated failure mechanism in metallic components and coated systems, where the effectiveness of the polymer coating is determined by the structural integrity and adhesion at the coating/substrate interface. This study investigated the corrosion fatigue interaction in AISI 410 steel with and without a poly(3-hexylthiophene)/poly (methyl methacrylate) (P3HT/PMMA) coating exposed to a 3 wt.% NaCl solution under four stress levels $\left(∆\sigma \right)$ at room temperature. Electrochemical noise (EN) was recorded during the test, the surface and interface were characterized using scanning electron microscopy (SEM), and the mechanical behavior was quantified using $da/dN$ vs. $∆K$ and $\sigma$ vs. N curves. The coated samples exhibited a wider potential range ($\approx \pm$400 mV) than the uncoated steel ($\approx \pm$200 mV), indicating localized electrochemical activity under the coating. SEM observations revealed microblisters at low stress levels and coating cracking at high stress levels, with localized substrate exposure, slip bands, and microcracks. Overall, the results showed that the corrosion fatigue is governed by electrochemical activity under the coating and cathodic delamination, which reduces adhesion, locally exposes the steel, and causes the initiation and propagation of cracks. Full article

We present a unified GPU-accelerated framework for real-time Smoothed Particle Hydrodynamics (SPH) fluid simulation with two-way rigid body coupling, secondary particle effects, and volumetric rendering, implemented entirely within the Unity game engine. The framework employs a weakly compressible SPH formulation with $O\left(n\right)$ count sort-based spatial hashing and introduces a signed distance field (SDF) coupling system that evaluates three representative geometric primitives, sphere, cylinder, and torus, of increasing topological complexity directly on the GPU. Bidirectional force exchange is achieved through lock-free atomic compare-and-swap impulse accumulation, enabling thousands of fluid particles to interact simultaneously with each rigid body without serialization. A GPU stream compaction–based secondary particle system generates and classifies foam, spray, and bubble effects in real time, while a volumetric rendering pipeline samples fluid density into a 3D texture for SDF-composited volume rendering without surface mesh extraction. A conditional kernel dispatch strategy eliminates GPU cycles for disabled subsystems, and dynamic buffer management reduces memory pressure through runtime allocation. The system sustains above 54 frames per second at four million particles on a consumer-grade GPU, with sub-linear frame time scaling and a $1.70\times$ speedup from dynamic buffer allocation over static pre-allocation. Full article

This paper addresses the impact of complex mining environments and the nonlinear dynamics of hinged mining trucks on reverse path tracking control for autonomous mining trucks. We propose a neural-network-based sliding mode control (NN-SMC)-based control strategy for reverse motion to improve tracking accuracy and robustness. First, a tractor–trailer dynamic model is built, and the force characteristics at the coupling joint are analyzed to derive the reverse interaction forces, which simplifies trailer modeling and avoids the influence of uncertain tractor parameters. Next, a control scheme matching the simplified model is developed, where an optimized sliding surface is designed and a neural network adaptively tunes control parameters to reduce chattering and improve adaptability to challenging conditions. Finally, hardware-in-the-loop tests validate the simulation results. Both simulation and experiments show that, compared with conventional SMC, the proposed method reduces lateral displacement error by 13.98% and heading error by 18.96%, demonstrating the effectiveness of the control approach. Full article

Seed-associated endophytes become active during germination, playing important roles as early colonizers of plant tissues and contributing to plant health while residing in a protective niche. In this study, we characterized a wheat-derived bacterial isolate, JunSE1L, to determine its functional traits and ecological role in the plant microbiome. The isolate was identified as Bacillus atrophaeus based on 16S rRNA analysis. JunSE1L exhibited nutrient-dependent plasticity in colony architecture, forming robust hydrophobic biofilms and pellicles under rich nutrient availability while swarming and forming thin, often dendritic colonies under defined nutrition. JunSE1L produced highly surface-active compounds that lowered the surface tension of water to 30 mN/m and released potent proteolytic and hemolytic compounds, thus equipping JunSE1L for antagonistic interactions, as examined against several fungal pathogens. JunSE1L inhibited Fusarium proliferatum and Mucor hiemalis in live-cell assays, while cell-free supernatant selectively inhibited M. hiemalis. JunSE1L was recovered from multiple plant compartments, including rhizosphere, rhizoplane, and aerial tissues, and was observed to emerge from cut plant tissues, supporting seed-endophyte mobilization upon germination to colonize distal tissues. Seed surface inoculation experiments with JunSE1L showed limited attachment at low cell densities and reduced seedling vigor at higher inoculum levels, indicating that inoculum density and native microbiome interactions influence seedling performance. Full article

The behavior of buried pipes constructed on slopes is of great importance for the safety and sustainability of infrastructure systems. Slope movements, landslides, and soil displacements can significantly affect pipe–soil interaction, creating additional stresses on the pipes. Therefore, accurately determining the behavior of pipes under slope conditions is essential for improving engineering designs and preventing potential damage. In recent years, advanced experimental methods have been widely used in soil mechanics and geotechnical engineering studies to determine deformation and displacement fields. In this study, the behavior of buried pipes in reinforced and unreinforced sloping soils was experimentally investigated. Particle Image Velocimetry (PIV), an advanced image-based technique, was used to analyze soil deformation and displacement fields based on the images obtained during the model experiments. The results indicate that geogrid reinforcement has a significant effect on the behavior of the buried pipe and the deformation patterns of the soil. The study is primarily intended as a mechanism-oriented experimental investigation rather than an extensive parametric optimization study. Through PIV-based full-field displacement analyses, the evolution of deformation zones and failure surfaces around the buried pipe was evaluated in detail. Full article

The Water-Sediment Regulation Scheme (WSRS) rapidly delivers large amounts of water, sediment, and nutrients to the Yellow River Estuary (YRE) in summer (wet season). However, how these abrupt environmental changes affect phytoplankton distribution through bottom-up versus top-down control mechanisms remains poorly understood. In this study, we examined the spatiotemporal distribution of environmental drivers, grazing pressure, and phytoplankton communities in surface and bottom layers of the YRE during WSRS. Our results indicate that the WSRS transitioned phytoplankton distribution from a relatively uniform pattern pre-WSRS to a highly heterogeneous one during the sediment regulation stage. Before WSRS, phytoplankton abundance peaked near the river mouth and was co-dominated by chlorophytes, cryptophytes, and diatoms in both layers. During the water regulation stage, abundance decreased across layers, with the surface community incorporating more dinoflagellates and the bottom layer transitioning toward higher diatom and lower chlorophyte proportions. Subsequently, vertical stratification intensified during the sediment regulation stage, characterized by a chlorophytes-dominated surface hotspot (with abundance 6.8-fold higher than pre-WSRS levels) in contrast to a depauperate bottom layer. Regression tree and redundancy analysis results showed that WSRS shifts phytoplankton regulation from a bottom-up state in the pre-stage to top-down dominance during the water regulation stage, and finally to a vertically stratified regulatory state in the SR stage, with top-down control in the surface layer and bottom-up control in the bottom layer. Our findings highlight that trophic interactions and physical processes play more critical roles than previously recognized in regulating phytoplankton distribution in estuaries subjected to high-intensity hydrological disturbances. Full article

Winter wheat production in the extremely arid oasis region of Xinjiang relies heavily on irrigation and fertilization, but conventional high-input management can induce luxury transpiration and non-productive water consumption, limiting the coordinated improvement of grain yield, water use efficiency (WUE), and economic benefits. To identify the threshold at which water–fertilizer inputs shift from efficient use to inefficient water consumption and to define a robust management range, a two-year field experiment was conducted in southern Xinjiang during the 2022–2023 and 2023–2024 growing seasons. Four irrigation levels, corresponding to 60%, 80%, 100%, and 120% of crop evapotranspiration (ET_c), and four fertilization levels were established to evaluate the effects of water–fertilizer interactions on canopy development, leaf gas exchange, evapotranspiration, yield, WUE, and economic benefits. Appropriate water and nutrient supply promoted canopy establishment and maintained higher photosynthetic capacity, thereby increasing grain yield, WUE, and net return. However, excessive inputs weakened yield gains and failed to synchronously improve WUE and economic benefits. Linear plateau models revealed clear thresholds in both the crop-stand scale evapotranspiration (ET)–dry matter accumulation (DM) relationship and the leaf-scale transpiration rate (Tr)–net photosynthetic rate (Pn) relationship. The seasonal ET thresholds were 504.59 and 553.87 mm in the two growing seasons, respectively, and the Tr threshold was 4.83 mmol m⁻² s⁻¹. Beyond these thresholds, additional water consumption was not effectively converted into photosynthetic assimilation or biomass accumulation, indicating luxury transpiration. Year-specific response surface analysis and TOPSIS evaluation showed that I3F3, namely 100% ET_c combined with 210–195–75 kg ha⁻¹ N–P₂O₅–K₂O, together with its adjacent range, sustained high grain yield, WUE, and economic benefits, with I3F3 achieving the best overall performance in both years. The intersection of the two-year high-performance regions further defined a robust interannual feasible range with an irrigation amount of 506.21–545.09 mm and a total fertilizer input of 369.54–628.33 kg ha⁻¹. Overall, maintaining water and fertilizer inputs within the I3F3-adjacent range can reduce non-productive water consumption and luxury transpiration risk while synergistically improving grain yield, WUE, and economic benefits in winter wheat. Full article

Background: The World Health Organization (WHO) declared a pandemic due to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the etiologic agent responsible for Coronavirus Disease 2019 (COVID-19). Although case numbers declined after the initial outbreak, Brazil experienced slight increases in COVID-19 cases in 2024 and 2025, underscoring the persistent need for effective therapeutic interventions. Recently, proxalutamide—an androgen receptor antagonist—has been proposed as a potential therapeutic agent against COVID-19, as supported by several clinical studies. Methods: In the present work, we aimed to elucidate the molecular interactions between proxalutamide and key proteins involved in the viral fusion and replication processes of SARS-CoV-2. Computational techniques, including molecular docking and molecular dynamics simulations, were employed. Results: Our analyses indicated a high success rate, with stable conformations and favorable binding affinity values for ACE2 (−8.90 kcal/mol) and TMPRSS2 (−9.28 kcal/mol), resulting in strong docking scores. Moreover, molecular dynamics simulations confirmed the stability of these complexes, as evidenced by consistent mean square deviation values, low structural flexibility, a stable radius of gyration, and maintained surface rigidity over a 100 ns simulation period. Conclusions: These combined docking and dynamics results suggest that proxalutamide interacts firmly with the active sites, indicating high binding affinity that may interfere with SARS-CoV-2 entry. Nevertheless, experimental validation and rigorous safety assessments are warranted to confirm this potential. Full article

Phytochrome-interacting factors (PIFs) were initially recognized as pivotal regulators of plant light signaling pathways. However, mounting evidence suggests that PIFs also exert significant influences on plant development and responses to stress. Here, we identified seven PIF genes in the potato genome and conducted comprehensive characterizations through phylogenetics, gene structure, conserved motif, synteny, chromosomal location analyses and cis-regulatory element. Transcriptome data and gene expression analysis showed that the StPIF4 gene was markedly induced by mannitol-induced water deficit. Additionally, the StPIF4 protein was primarily localized in the nucleus and plasma membrane. In order to explore the function of the StPIF4 gene under mannitol-induced water deficit, the StPIF4 gene was cloned, and several StPIF4 overexpression (OE) lines (OE-8, OE-10, and OE-11) and three RNA interference (RNAi) transgenic lines (RNAi-5, RNAi-9, and RNAi-11) were obtained. The OE lines displayed notable enhancements in various growth parameters such as plant height, leaf number, branch number, fresh weight, dry weight, total root length, root surface area, number of root forks, and number of root tips under mannitol-induced water deficit compared to the wild-type (WT) lines, whereas these parameters were significantly decreased in the RNAi lines. The activities of antioxidant enzymes (SOD, POD, CAT) and the accumulation of proline and soluble sugars were also significantly increased under mannitol-induced water deficit, whereas the levels of thiobarbituric acid reactive substances (TBARSs) and reactive oxygen species (ROS), including hydrogen peroxide (H₂O₂) and O₂⁻, were significantly reduced in the OE lines compared to WT plants under mannitol-induced water deficit. Moreover, the stomatal aperture of the leaves and the water loss rate in the leaves of the OE lines were significantly reduced under mannitol-induced water deficit compared to the WT plants, whereas for the RNAi lines they were significantly increased. In addition, the overexpression of StPIF4 also upregulated expression of drought-responsive genes and ABA content under mannitol-induced water deficit. Collectively, these results highlight the positive role of the StPIF4 gene in enhancing potato tolerance to mannitol-induced water deficit by decreasing stomatal aperture, enhancing ROS scavenging and mitigating oxidative damage. Full article

The growing demand for sustainable smart packaging arises from the urgent need to preserve food quality and minimize environmental waste. In this study, multifunctional gelatin-based pH-responsive indicator films were fabricated by incorporating anthocyanins extracted from Hibiscus x archeri W Watson (HAE) and zinc oxide nanoparticles (ZnO-NPs). The incorporation of HAE and ZnO-NPs enhanced surface hydrophobicity, as evidenced by an increase in the water contact angle from 99° to 106°. The Fourier transform infrared (FTIR) analysis verified the lack of new chemical bond formation, indicating that the interactions among components were primarily physical in nature. Distinct colour transitions in buffer solutions of differing pH demonstrated the films’ colorimetric behavior. The films exhibited strong antimicrobial activity against Listeria monocytogenes (18.961 mm), Salmonella typhimurium (18.969 mm), and Aeromonas hydrophila (18.237 mm), whereas the neat gelatin film showed no inhibitory zone. The films also demonstrated superior UV-blocking capacity, with an opacity value (1.34 a.u/mm) compared to the control gelatin film (0.79 a.u/mm). Notably, fish fillets wrapped with the films remained fresh for up to 10 days, compared to day 4 for the unwrapped samples. These findings highlight the considerable potential of multifunctional, active and intelligent packaging for food preservation and real-time freshness monitoring. Full article

Understanding the spatial heterogeneity of deep soil organic matter is critical for terrestrial carbon cycling, yet its driving mechanisms remain elusive due to a historical research bias toward surface layers. This study develops a 3D spatial prediction and mechanistic framework for Soil organic matter(SOM) across a 0–200 cm profile in the Mollisols of the Songnen Plain, Northeast China. By integrating systematic sampling with Random Forest modeling and a comprehensive suite of multi-source environmental covariates, we identified pronounced vertical stratification in SOM distribution and its governing factors. Our results reveal that surface SOM is primarily driven by climate, vegetation, and anthropogenic activities, whereas deep soil organic matter is dictated by intrinsic physicochemical properties and surface matrix compositions. Furthermore, significant interaction effects between topography and soil attributes further enhance SOM accumulation. This research clarifies the depth-dependent processes of carbon stabilization, providing a robust scientific basis for the targeted conservation and carbon sequestration enhancement of Mollisols. Full article

Electrically assisted processing of metallic materials has emerged as a promising paradigm for reducing deformation resistance while concurrently tailoring microstructure and service-related properties under coupled electrical, thermal, and mechanical fields. This review focuses on deformation-dominated and surface-strengthening scenarios, examining recent advances from three interconnected perspectives: fundamental mechanisms, microstructural evolution, and property responses. Available evidence suggests that Joule heating typically constitutes the dominant contribution under high-duty-cycle or near-steady-state current conditions, whereas non-thermal electroplastic effects become increasingly pronounced under short-pulse, high-current-density, and temporally decoupled loading regimes. Current assistance can accelerate recovery and recrystallization, refine grain structure, modify crystallographic texture, and alter phase transformation and precipitation kinetics. Additionally, it can relax or redistribute residual stresses while reducing flow stress and forming forces. In select hybrid surface treatments, these microstructural modifications translate into enhanced resistance to fatigue, wear, and corrosion. Nevertheless, the available evidence precludes a single universal explanation, given that current crowding, defect-selective heating, electron–dislocation interactions, and magnetic effects operate concurrently, with their relative importance varying across material systems and processing conditions. Moving forward, establishing a unified framework that links electrical parameters, defect evolution, microstructure, and performance is imperative, with focused efforts on the quantitative delineation of thermal and non-thermal contributions, predictive constitutive modeling, residual stress stability, and industrial scalability. Full article