Search Results (1,879)

This study examined Kefiran, an exopolysaccharide derived from milk kefir grains, as a novel biopolymer for encapsulating phenolic extracts from sunflower cake and its antimicrobial properties in the development of natural and functional food ingredients. Kefiran was obtained from kefir grains using three extraction protocols: hot water (M1), hot water with 30% trichloroacetic acid (M2), and mild heat combined with ultrasound at 60 °C (M3). The ultrasound-assisted method produced the highest carbohydrate concentration. Spectrophotometric assays (phenol–sulfuric and Bradford), Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, and water-holding capacity were employed to characterize the composition, structure, and morphology of the extracts, revealing well-preserved polysaccharide fingerprints and a highly porous microstructure, consistent with their potential application in food systems. Kefiran was then evaluated as an encapsulating agent in complex coacervation at pH 3.75, using three Kefiran-based wall formulations (M1, M2, and M3) with gum arabic and whey protein isolate (WPI) as co-wall materials, and their performance was compared with gum arabic and WPI controls. Across formulations, coacervate microcapsules achieved high encapsulation efficiencies (83–93%), tunable particle sizes, and predominantly negative zeta potentials, indicative of good colloidal stability. The Kefiran extract and coacervate microcapsules demonstrated significant antioxidant and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans, with minimum inhibitory concentrations ranging from 250 to 1000 µg/mL. The findings support ultrasound-extracted Kefiran as a multifunctional biopolymer suitable for bioactive delivery and as a natural antimicrobial component in advanced functional food formulations. Full article

The valorization of winemaking by-products is a sustainable strategy consistent with circular bioeconomy principles and current public health priorities. This study aimed to evaluate residual oenological yeast sediment from local Moldovan grape varieties, Viorica and Fetească Regală, as a multifunctional ingredient and partial fat replacer in muffins. Sunflower oil was replaced with wine lees (WL) at 20%, 35%, and 50%, and the obtained products were analyzed in terms of physicochemical, nutritional, microbiological, colorimetric, and sensory characteristics. WL incorporation reduced the caloric value by up to 10% and decreased lipid content, while contributing to higher protein and dietary fiber levels. Moisture values remained within acceptable limits, whereas titratable acidity increased with the substitution level (p < 0.05). Muffin density showed a slight increase, and water absorption capacity improved markedly, reaching 269%, mainly due to the fiber-rich composition of WL. Color analysis indicated reduced lightness and increased redness, associated with yeast pigments and thermal reactions during baking. Microbiological results showed lower total viable counts with increasing WL addition; however, the 50% substitution level exceeded the permissible limits for yeasts and molds. Sensory evaluation indicated that the muffin with 20% WL was the most acceptable sample. Overall, WL may be considered a promising sustainable ingredient for developing reduced-fat muffins with improved nutritional value. Full article

Sunflower (Helianthus annuus) can potentially be used for uranium (U) phytoremediation. However, the influence of arbuscular mycorrhizal fungi (AMF) on key rhizosphere processes and plant U uptake remains insufficiently researched. We hypothesized that AMF inoculation could enhance sunflower tolerance to U stress by improving plant physiological performance and modifying rhizosphere properties. To test this hypothesis, this study examined the effects of AMF (Funneliformis mosseae, Glomus etunicatum, and their co-inoculation) on sunflowers under U stress, encompassing plant growth and physiological traits, rhizosphere properties, enzyme activities in the rhizosphere soil, uranium speciation in the rhizosphere soil, and the accumulation and distribution of uranium within the plant. Results showed that AMF successfully colonized the roots, enhancing plant growth, biomass, and gas exchange, while improving photosynthetic efficiency and reducing non-photochemical quenching. In the rhizosphere, AMF elevated soil respiration, organic matter, dissolved organic carbon, and microbial biomass carbon; improved phosphatases, urease, catalase, and sucrase activities; also reshaped U speciation, increasing exchangeable and carbonate-bound fractions while decreasing those bound to organic matter, Fe/Mn oxides, and residual phases. Moreover, AMF reduced U concentration in leaves and stems, promoted U retention in belowground tissues, and significantly lowered the U translocation factor. These findings demonstrate that AMF inoculation improves sunflower tolerance to U stress by enhancing physiological performance, modifying rhizosphere properties, and immobilizing U in roots, supporting its potential use in phytoremediation strategies for U-contaminated environments. Full article

Background: An increasing number of commercially available drug substances and bioactive ingredients are characterized by poor flowability. Inadequate flow properties may lead to material blockage during transport within production lines, as well as the formation of air voids within the bulk. Such phenomena can disrupt the technological process and may even result in batches that fail to meet quality requirements. Therefore, ensuring adequate powder flow is of utmost importance in the manufacture of health-related products. Methods: Binary mixtures were prepared using one of four model substances (ibuprofen, metamizole sodium, mefenamic acid, or sunflower lecithin) combined with a glidant (colloidal silica, precipitated silica, or tricalcium phosphate). The glidant content ranged from 0.5 to 10.0% w/w depending on the model substance, and mixing was carried out for 5–30 min. The resulting binary mixtures were evaluated for flow properties using the angle of repose method, and in selected cases, bulk density was also determined. Results/Conclusions: The study demonstrated that powder flow improvement depended not only on the glidant but primarily on the properties of the host material (particle size, shape, and bulk density). Coarser powders such as ibuprofen responded well to low glidant levels, although excessive silicon dioxide caused oversilication. Metamizole sodium showed progressively better flow with increasing particle size and density, and tribasic calcium phosphate further improved performance, particularly with longer mixing times. Very fine or cohesive powders, such as mefenamic acid and sunflower lecithin, showed limited response to silica-based glidants, whereas tribasic calcium phosphate proved more effective and additionally increased bulk density. Overall, no universal glidant strategy was identified; effective flow enhancement requires a tailored approach based on specific powder characteristics. Full article

Soil salinization commonly prevails in global arid and semi-arid areas, shrinking farmland and endangering ecological, agricultural and social sustainability. Therefore, it is essential to develop effective strategies for salinized soil remediation. In this study, soil samples were collected from Nanliang Farm in Yinchuan, China. Compound microbial strains (CMS) and humic acid (HA) were selected as soil amendments. A total of eight treatments with different application rates of CMS and HA were set up in pot cultivation experiments, where oil sunflower was planted. The results showed that both amendments effectively elevated soil water content and chlorophyll content, as well as multiple physiological indices of sunflower. Meanwhile, they decreased soil total salinity, proline content and malondialdehyde (MDA) content. For single humic acid treatments, Treatment F1 achieved the optimal amelioration effect: it reduced soil total salinity by an average of 24.34%, and increased sunflower plant height, leaf area and aboveground fresh weight by 5.84%, 95.01% and 77.40%, respectively. Among the single CMS treatments, Treatment S3 performed best, with an average reduction of 31.04% in soil total salinity, and increases of 5.66%, 2.85% and 8.16% in plant height, leaf area and aboveground fresh weight correspondingly. Notably, among all eight groups, the control group CK1 exhibited the most prominent improvement effect, which was significantly superior to F1 and S3. This finding suggests that long-term application (one year or more) of CMS can produce an especially strong ameliorative effect on salinized soil. Full article

Canned fish products enable long-term preservation of fish, a vital source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Despite research on lipid composition, gaps remain in understanding the bidirectional fatty acid (FA) exchange between fish muscle and coating media during processing and storage. After a systematic literature search across five databases (PubMed, Scopus, Web of Science, Wiley Online Library, Cochrane Library), 20 studies were included examining FA profiles across fish species, filling media (vegetable oils, brine, tomato sauce), and storage durations (up to 5 years). Five studies showed that n-3 FAs migrate from fish to the filling medium, enhancing its nutritional value, while fish muscle absorbs FAs from the oil, increasingly resembling the filling medium. The use of n-6 FA-rich oils (sunflower, soybean) lowered the n-3/n-6 ratio in flesh. Conversely, aqueous media (brine) and tomato sauce maintained better ratios. EPA and DHA content generally decreased due to canning and storage, with retention varying by fish species, filling medium, and sterilization method. This review underscores significant FA exchange between fish and filling media, confirming bidirectional lipid interchange during processing. To optimize health benefits, aqueous packing media are recommended to preserve lipid profiles or to consume the covering oil to recover nutrients. Further research is needed on other factors altering FA content in canned fish such as environmental and geographical variables (including catching season), pre-canning preparation and sterilization steps (such as freezing, steaming, and frying), sterilization conditions (time, temperature, F₀ value) and lipid oxidation induced by thermal processing. Full article

Traditionally, various herbs and spices are used to enhance the flavor and aroma of vegetable oils, but also to improve their nutritional value and stability. The aim of this work was to investigate the effects of sea fennel, an aromatic edible Mediterranean halophyte plant, leaf infusion, on the chemical composition of four unrefined edible vegetable oils (olive, sunflower, sesame and flaxseed oil). During the 90-day storage period, the quality parameters of the oils (peroxide value, free fatty acids and fatty acid profile), as well as their volatiles, were monitored. Free fatty acids and peroxide values increased in all samples, with the greatest increase in the olive oil (11% and 45%, respectively), while the effect on the fatty acid profile was negligible. Gas chromatography-mass spectrometry analysis showed the effect of oil aromatization by sea fennel components and confirmed the differences between oil samples. The results suggest that the addition of sea fennel to vegetable oils leads to changes in their chemical composition, and the parameters tested varied between the oils used. Full article

This paper studies eigenvector constraints for adjacency hypermatrices of uniform hypergraphs using matroid circuits. Building on Pearson’s circuit relations, we define the circuit constraint space $\mathcal{L}\left(H\right)$ as the span of all Pearson relation vectors associated to circuits of the transversal matroid of the Levi graph. This yields a basis-independent parameter count $\pi \left(H\right):=\left|V\right(H\left)\right|-dim\left(\mathcal{L}\right(H\left)\right)$, which bounds the degrees of freedom for transformed eigenvectors associated with nonzero eigenvalues. We compare $\mathcal{L}\left(H\right)$ with basis-dependent spaces ${\mathcal{L}}_B\left({\mathsf{\Lambda }}_B\right)$, obtained by choosing one Pearson relation from each fundamental circuit relative to a matroid basis. Under a triangular independence condition and constraint-efficiency, we prove $\pi \left(H\right)=rank\left(M\right(H\left)\right)$. We verify this equality for loose paths, hyperstars, and sunflowers. We also develop a constraint ideal interpretation, study symmetry via automorphism actions, and use the resulting constraints to construct structured initializations for shifted higher-order power methods. The numerical examples illustrate how these structured initializations can influence observed iteration dynamics, without establishing a general convergence or acceleration theorem. Full article

The question of verification of food quality has stood before scientists since ancient times, and, nowadays, the advances in science and technology have made it a very challenging task. Laser-induced fluorescence (LIF) spectroscopy has become a very useful instrument for sample characterization. Nevertheless, analysis of complex multi-component spectra is difficult to approach. In recent years, the capabilities of artificial intelligence have attracted a lot of attention, as they open doors to efficient solutions of many problems that otherwise require a lot of time, effort, expenses and often inspiration. In the present work, we use LIF spectra of mixtures of sunflower and extra virgin olive oils with different concentrations and apply neural network (NN) algorithms with the aim of improving the strategies for concentration determination. Two different approaches have been applied and their output has been compared and commented. More specifically, the task of concentration recognition has been targeted as a classification and as a fitting problem. We formulate four diagnostic parameters with biochemical meaning and compare the NN performance when training with raw spectra and with the diagnostic parameters. The correct choice of appropriate diagnostic parameters is of importance from the point of view of biochemical interpretability and analysis, whereas “black box” full-spectra training might be beneficial for end-user applications. Our results show that these methods perform well even with very scarce data and outline preliminary strategies for defining diagnostic criteria. Full article

Leptolegnia chapmanii is an oomycete pathogen of mosquito larvae. We investigated whether nutritional factors promoting cyst germination in vitro are associated with early infection events and instar-specific susceptibility in Aedes aegypti. Cysts of the Brazilian isolate ARSEF 12829 germinated rapidly in soybean seed extract, sunflower seed extract and minimal medium supplemented with yeast extract, whereas basal minimal medium did not promote germination. In sunflower seed extract, germination increased significantly with incubation time; in minimal medium, germination at 24 h was much higher with ≥0.2% yeast extract than with 0.1%. In third-instar larvae, a few cysts attached to the cuticle during the first 30–60 min, with no external germ tubes observed. At 3 h, melanized hyphal structures were detected in the midgut, and histological sections showed germinated and ungerminated cysts in the endoperitrophic space, with hyphae crossing the peritrophic matrix and midgut epithelium toward the hemocoel. Mortality increased with cyst concentration and exposure time and decreased with larval instar. At 3.3 × 10³ cysts/mL, final mortality reached 100% in L1–L3 and 91.2% in L4 larvae. These results link rapid cyst germination with early midgut invasion and high larvicidal activity. Full article

The purpose of this work is to investigate the use of high-resolution ($~100$ m) surface soil moisture (SSM) maps derived from Sentinel-1 (S-1) and Sentinel-2 (S-2) data to identify irrigation events occurring in the Riaza irrigation district (Castilla y León region, Spain) from 2017 to 2021. The proposed method is based on the application of the Constant False Alarm Rate (CFAR) algorithm, which is an adaptive and unsupervised thresholding algorithm traditionally used for target detection in SAR images. This algorithm uses a sliding window approach that allows an adaptive threshold estimate for each pixel of the image, depending on the distribution of the surrounding pixels. The analysis was carried out on fields cultivated with maize, sugar beet and sunflower. Results show that the Overall Accuracy (OA) of the detection mainly depends on the time span (TS) between the S-1 passage and the irrigation event, the acquisition timing and the development stage of the vegetation. Indeed, the OA reaches a mean of $78\%$ and $70\%$, respectively, for the $6$ a.m. and $6$ p.m. acquisitions, when the irrigation events occur within $36$ h before the S-1 passage, and it follows a downward trend as the TS increases. On the other hand, when the vegetation reaches the mature stage, the mean OA decreases respectively to $56\%$ and $52\%$. Stemming from the event detection, the study explored the estimation of the total irrigated area in the early growing season, showing promising agreement with in situ data, as evidenced by the low Relative Error ($E_r\approx 5.6\%$). Additionally, the analysis revealed a significant correlation between field-scale mean SSM and irrigation depths ($R=0.89$). Full article

Magnetic materials have demonstrated considerable potential for applications in the field of energy storage. Spinel-type CoFe₂O₄ possesses both good redox activity and structural stability, but its magnetism may affect the electrochemical performance. During the high-temperature carbonization and activation processes, the magnetism is significantly weakened, thereby exerting only a limited effect on device performance. To address the issues of high cost and poor environmental friendliness of traditional electrode materials, two types of waste biomass, namely banana peels and sunflower seed shells, were employed as carbon sources for the preparation of Y-doped CoFe₂O₄/carbon composites in this study. Y-doped CoFe₂O₄/banana peel carbon was used as the positive electrode, while Y-doped CoFe₂O₄/sunflower seed shell carbon was used as the negative electrode. The results indicate that the CoFe₂O₄/BPC cathode doped with 0.4% Y has the best performance, with a specific capacitance of 1788 F/g at 1 A/g and a retention rate of 98% after 10,000 cycles. In addition, the SSPC anode exhibited a specific capacitance of 350 F/g and excellent cycling stability. The assembled device achieved a specific capacitance of 190 F/g at 1 A/g and a capacitance retention rate of 83.6% after 10,000 cycles at 5 A/g, demonstrating good energy density, power density and cycling stability. This research provides experimental evidence for the development of low-cost supercapacitors based on biomass. Full article

Fusarium spp. are active producers of mycotoxins that enter the food chain and pose risks to human health. Identifying pathogenic agents is a key step in developing disease management strategies. For the first time in Bulgaria, we identified eight Fusarium species in wheat, harvest 2024–2025, through the application of DNA barcoding. For a genetic marker and construction of phylogenetic tree, the protein-coding gene β-tub was chosen. Among 26 identified isolates, F. sporotrichioides (42.3%) dominated, followed by F. proliferatum (23.1%), F. avenaceum (7.7%), F. armeniacum (7.7%), and F. poae (7.7%). F. tricinctum (3.8%), F. oxysporum (3.8%), and F. equiaseti (3.9%) were weakly expressed. Phylogenetic analysis classified the isolates into five species complexes: FSAMSC, FFSC, FTSC, FIESC, and FOSC and highlighted the genetic distances between them. Molecular genetic analysis showed that 84.6% of the wheat samples contained only one species of Fusarium, and in 15.4% the co-presence of two species was established. The largest share was in samples with a low infestation of 2–4%, which represented 35% (n = 32) of all positives. No statistically significant difference was found between varieties and contamination level, but a statistically significant positive correlation was demonstrated by the preceding crop (rapeseed, sunflower, and maize). Full article

Amid growing water scarcity, assessing agricultural water consumption and crop water footprint has become increasingly critical. This study aims to assess the water footprint of crops within the Shu–Talas River Basin, disaggregated into green and blue components. Using meteorological data from the 2000–2024 period, reference evapotranspiration (ET₀) and actual crop evapotranspiration (ET_c) were calculated according to the FAO methodology. The water footprint (WF_green, WF_blue, and WF_quant) was determined based on crop evapotranspiration, effective precipitation, and crop yields for maize, sugar beet, sunflower, and potato. It was found that total water consumption during the growing season ranges from 650 to 950 mm, with the blue water share exceeding 80%, reflecting the high dependence of agricultural systems on irrigation. The minimum WF_quant values were observed in sugar beet, while the maximum WF_quant values were recorded for sunflower. The study identifies crop yield, rather than absolute water consumption, as the key factor in water footprint formation. These findings and established patterns can be utilized to optimize cropping patterns and support sustainable agricultural water management in arid regions. Full article

Specialty oilseeds, encompassing herbaceous (sunflower, flaxseed, sesame) and woody (Camellia oleifera, walnut, olive) species, serve as important sustainable sources of plant proteins that are inherently enriched with structurally diverse endogenous polyphenols such as chlorogenic acid, lignans, catechins, and ellagitannins. During processing, these polyphenols drive covalent or non-covalent interactions that profoundly reshape the physicochemical and functional properties of the resulting food systems. While prior reviews have largely remained descriptive or focused on single commodities or model proteins, this work provides the first critical, multiscale synthesis across herbaceous and woody oilseeds. We systematically compare polyphenol diversity, delineate the continuum from reversible non-covalent association (specific residue-level vs. non-specific surface-mediated) to irreversible covalent coupling, and establish a “structure–interaction–function” framework that explicitly defines a condition-dependent “Processing Window”. Within this window, moderate interactions enhance interfacial viscoelasticity, oxidative stability, foaming, and emulsification; excessive cross-linking, however, impairs solubility, digestibility, and sensory quality. By integrating experimental spectroscopy (UV-vis, FT-IR, CD, ITC), microscopic imaging, and computational simulations (molecular docking and dynamics), we map residue-level binding modes directly to macroscopic functional outcomes. Furthermore, the review evaluates the engineering potential of these complex systems in frontier applications such as antioxidant emulsions and active packaging. By explicitly identifying evidence boundaries and quantitative knowledge gaps in endogenous matrices, this work provides a comprehensive theoretical framework for the precision design and valorization of specialty oilseed-derived functional ingredients. Full article