Search Results (40)

Fruit sunburn is a major abiotic stress limiting apple production worldwide, with losses potentially reaching 50% due to climate change-driven heat events. This study aimed to evaluate sustainable strategies to mitigate or reduce sunburn on ‘Gala Galaxy Selecta’ apple trees. Field trials conducted in summer 2021 compared eight treatments: silicon-based application (Eckosil^®), foliar fertilization with algae extracts, macro- and micronutrients, and amino acids, increased irrigation (+35% ET_c), mineral particle films (Surround^®, Vegepron Sun^®, Agrowhite^®, Sunstop^®), and an untreated control. Randomized block designs with replicates were used. Agronomic parameters, including particle film coverage, trunk cross-sectional area, yield, and fruit quality (color, sunburn incidence, firmness, soluble solids content, dry matter, starch), were measured at harvest. Physiological responses, such as net photosynthesis, maximum quantum yield of Photosystem II, specific leaf area, fruit surface temperature, photoprotective pigments, antioxidants, and heat shock protein gene expression, were also assessed. Foliar fertilization, Agrowhite^®, and water reinforcement produced the highest yield per trunk cross-sectional area, with increased soluble solids content and enhanced red pigmentation. Surround^® minimized sunburn incidence but reduced photosynthetic activity, as did Vegepron Sun^®. Agrowhite^® balanced sunburn protection with maintenance of fruit quality and physiological function. These findings provide practical guidance for growers to select effective treatments, balancing sunburn mitigation, fruit quality, and tree physiological performance, while offering researchers insights into integrating agronomic and physiological strategies for climate-resilient apple production. Full article

Background and Clinical Significance: Hell’s Itch is a rare, intensely uncomfortable post-sunburn condition with burning pruritus emerging 24–72 h after UV exposure. This condition often goes unrecognized and is frequently misdiagnosed by healthcare providers due to a lack of knowledge and familiarity. Standard antipruritic measures are often ineffective, and patients frequently rely on anecdotal self-management. Case Presentation: Three healthy adult males between 23 and 28 years old experienced multiple episodes of delayed-onset intense pruritus following moderate-to-severe sun exposure. The patients experienced a burning or stinging pain which they described as “fire ants” or “thumbtacks,“ and their symptoms started between 24 and 72 h after sun exposure without any rash or fever symptoms. The patients did not achieve symptom relief from standard treatments which included oral antihistamines and topical lidocaine, NSAIDs, aloe vera, and cold compresses. The patients received β-alanine treatment through pre-workout supplements or pure powder after consulting non-clinical sources. Each patient ingested β-alanine and reported rapid relief (itch 8–10/10 → 1–2/10) lasting 2–3 h. The only adverse effect reported by one patient was mild paresthesia. Conclusions: This case introduces β-alanine as a potential off-label therapy for Hell’s Itch and emphasizes the psychological burden and clinical complexity of the condition. While anecdotal, further study is needed to elucidate the mechanism of action of β-alanine in relieving symptoms of Hell’s Itch, as well as assess safety and efficacy in controlled settings. Increased clinical awareness of Hell’s Itch may reduce patient distress and improve management strategies. Full article

The timing of fruit bag removal is important for achieving optimum fruit quality, coloration, and visual appearance. Therefore, this study investigated the effects of fruit bag removal timing on fruit quality and color and the occurrence of physiological disorders in ‘Arisoo’ apples. Fruits were bagged in two-layer paper bags, which were removed 30, 20, and 10 days before harvest (DBH). Unbagged fruits served as the control. The incidence of fruit cracking, sunburn, and pathogen infection was highest in the unbagged group, followed by the group with bag removal at 30 DBH, and lowest in those with bag removal at 20 and 10 DBH. However, bag removal at 10 DBH significantly reduced fruit weight and soluble solids content but increased firmness. Additionally, bag removal at 10 DBH resulted in poorly colored fruits with higher chlorophyll and lower anthocyanin and carotenoid pigments and lower expression levels of pigment-related genes, including anthocyanin-, carotenoid-, and chlorophyll degradation-associated genes, compared with those in the other treatment groups. Bag removal at 30 and 20 DBH did not significantly affect fruit quality or coloration, but it did affect fruit size. Overall, this study serves as a reference for determining the optimal timing of fruit bag removal to enhance the quality and coloration of ‘Arisoo’ apples. Full article

Recent climatic extremes, characterized by hot and dry summers, threaten grape yield and berry composition, increasing the need for sustainable mitigation strategies. In this study, a fruit-zone cooling system was tested to reduce sunburn damage and improve vine performance. The system integrates proximal sensors and an automatic misting actuator, triggered when the air temperature exceeds 35 °C. Over two seasons (2022–2023), trials were conducted on Pignoletto vines subjected to four treatments: control (C), misted without defoliation (C + FOG), defoliated (DEF), and defoliated plus misted (DEF + FOG). The effects on microclimate, yield, berry sunburn, and berry composition were evaluated. Misting consistently reduced both air and berry temperature. Treated vines showed increased yield, mainly due to reduced sunburn and higher cluster weight. Although no clear differences in technological maturity were observed, misted vines tended to retain higher acidity under extreme heat. Flavonol synthesis was unaffected by cooling but stimulated by increased light exposure, being higher in defoliated vines. Volatile compounds analysis highlighted misting’s moderating effect on oxidative stress and aroma profile shifts, particularly during the hotter season. Overall, the cooling system proved effective in mitigating summer stress, offering a promising tool for preserving yield and berry composition in white cultivars under climate change. Full article

Background: Patients diagnosed with melanoma are at increased risk of developing multiple primary melanomas (MPMs). Identifying clinical and genetic factors associated with MPM is critical for implementing personalized surveillance strategies. This study aims to describe the clinical, histopathological, and genetic characteristics of patients with MPM managed in a tertiary hospital and to contextualize findings within the current literature. Methods: We conducted a retrospective review of patients diagnosed with two or more primary melanomas between 2010 and 2023 at a tertiary dermatology unit. Demographic data, personal and family cancer history, phototype, melanoma characteristics, genetic testing, staging, treatments, and outcomes were collected. These data were compared with findings from the recent literature. Results: Thirteen patients (ten males, three females; median age: 59 years) were found to have a total of 33 melanomas. Most patients had Fitzpatrick phototype II and no immunosuppression. The number of melanomas per patient ranged from two to five. Synchronous lesions were observed in two patients. Common locations included the trunk and extremities. Histologically, 57% were in situ melanomas, and subsequent melanomas were generally thinner than the index lesion. Two patients showed progression to advanced disease. One patient was positive for MC1R mutation; the rest were negative or inconclusive. Additional phenotypic and environmental risk factors were extracted from patient records and are summarized as follows: Ten patients (76.9%) had Fitzpatrick skin phototype II, and three (23.1%) had phototype III. Chronic occupational sun exposure was reported in four patients (30.8%), while five (38.5%) recalled having suffered multiple sunburns during childhood or adolescence. Eight patients (61.5%) presented with a total nevus count exceeding 50, and five (38.5%) exhibited clinically atypical nevi. None of the patients reported use of tanning beds. Conclusions: Our findings are consistent with the existing literature indicating that patients with MPM often present with thinner subsequent melanomas and require long-term dermatologic follow-up. The inclusion of genetic testing and phenotypic risk factors enables stratified surveillance and supports the application of personalized medicine in melanoma management. Full article

Orah mandarin (Citrus reticulata cv. Orah) lacks systematic grading treatment after harvesting, resulting in a high fresh fruit loss rate and affecting the economic value. There are many drawbacks to traditional manual and mechanical sorting. Therefore, intelligent, rapid, non-destructive surface defect detection technology is significant. In addition to the fruit size, surface defects (e.g., canker, sunburn) are another important criterion for grading fruit. To overcome the challenges in detecting surface defects of orah mandarin, like multi-scale features, significant size differences, and slow convergence speed, we propose the YOLOv8-Orah detection model based on YOLOv8n. Path Aggregation Network (PANet) is replaced by a Focusing Diffusion Pyramid Network (FDPN), and the Diffusion and Spatial Interaction (DASI) module is introduced to effectively fuse and enhance features of different scales and improve detection accuracy. The Bottleneck in the C2f module is replaced by the Hybrid Dilated Residual Attention Block (HDRAB) module to reduce missed detections and false detections. We also introduce the NWD-CIoU joint bounding box loss to accelerate the convergence speed and improve the detection accuracy of small defects. The experimental results show that the improved YOLOv8-Orah model performs well in terms of precision, recall, and average precision, reaching 81.9%, 78.8%, and 84.2%, respectively. Compared with the original YOLOv8n, the improved model increased by 4.0%, 1.7%, and 3.0%, respectively. Meanwhile, the parameter count decreased by 7.76%. Compared with other mainstream models, YOLOv8-Orah achieves a good balance between detection accuracy and computational efficiency. The results technically support defect detection in postharvest orah mandarin and real-time grading of their quality. Meanwhile, it can promote the intelligent development of the bergamot industry. Full article

Considering climate change predictions, it is logical to anticipate detrimental effects on the mandarin tree, an essential citrus crop. Therefore, scientists should promptly focus on developing methods to enhance its resistance to climatic stress effects such as sunscald. This study assesses the strategies employed in ‘Balady’ mandarin trees when covered by shading nets of varying colors and percentages (white 50%, green 50% or 63%, black 50% or 63%), as well as the application of reflective materials (kaolin at 4% and CaCO₃ at 3%) on the micro-climate of orchards, leaf, and fruit surface temperatures, fruit sunburn%, productivity, and fruit quality. The results indicated that shade nets effectively reduced temperature and enhanced humidity, especially during the period from June to September, when compared to open-field treatments. Black shade nets, particularly those with a shading level of 63%, demonstrated the most notable decrease in canopy temperature and an elevation in humidity, surpassing the performance of green and white shade nets. The present study found that shade nets and reflecting materials like kaolin and calcium carbonate significantly reduced fruit sunburn. Trees without shade had a sunburn rate of 8.74%, while those with shade treatments suffered no sunburn. Kaolin foliar spray at a concentration of 4% and calcium carbonate at a concentration of 3% reduced sunburn incidence to 3.64% and 7.32%, respectively. These treatments also reduced the intensity of sunburn. All treatments increased fruit yield % compared to the control and yield efficiency (kg/m²), especially the trees covered with white shade net of a 50% shading rate provided the highest values (43.70 and 40.17%) and (5.24 and 5.47 kg/m²) compared to other treatments in both seasons, respectively. Trees covered with a white shade net of a 50% shading rate, followed by a green shade net of 50% and a 63% shading rate, as well as a black shade net of 50% and a 63% shading rate, tended to improve the physical and chemical fruit properties. Therefore, it could be recommended that trees be covered with a white shade net of a 50% shading rate or a green shade net of a 50 and 63% shading rate in summer months due to its beneficial impact on mitigating fruit sunburn damage and enhancing the productivity and quality of ‘‘Balady’’ mandarin trees. Hence, shade nets can be a beneficial technology to protect citrus fruits from sunburn without affecting fruit quality in commercial citrus farms. Full article

Fruit skin color and physical quality are important for customer acceptability and market value. Therefore, this study aimed to evaluate the effect of pneumatic defoliation on the fruit quality, coloration, and anthocyanin content of ‘Fuji’ apples. Apple trees were subjected to no defoliation (control) and defoliation at low (0.6 bar) and high (0.9 bar) air pressure 20 days before harvest at 1 km/h of tractor speed. High-defoliation treatment increased the leaf damage rate but did not significantly affect the defoliation rate compared to low-defoliation treatment. Additionally, photosynthetically active radiation and solar irradiance inside the tree canopies were highest in the high-defoliation group, followed by the low-defoliation and control groups. With the exception of higher firmness in the high-defoliation treatment, pneumatic defoliation treatments had little effect on fruit size and weight, titratable acidity, soluble solids content, the starch pattern index, and the sunburn incidence of fruit. Compared with that of the control group, both defoliation treatments significantly increased the a* and C values and decreased the h^o values of the fruit color. Moreover, both defoliation treatments significantly increased anthocyanin content and upregulated the anthocyanin biosynthesis genes (MdPAL, MdCHS, MdCHI, MdF3H, MdANS, MdANS, MdUFGT) and the transcription factor (MdMYB10). A Pearson′s correlation analysis also showed that anthocyanin production was strongly correlated with each of the anthocyanin biosynthesis genes, especially in the pneumatic defoliation treatments. Conclusively, the results show that pneumatic defoliation at low pressure bars could be an effective strategy for improving the red coloration of ‘Fuji’ apples. Full article

Excessive ultraviolet (UV) exposure can lead to sunburn, characterized by skin barrier damage, inflammation, pain, and an increased risk of skin cancer. Recombinant collagens have gradually attracted attention due to their high purity, low immunogenicity, batch-to-batch consistency, and excellent solubility. Additionally, the type of dressing significantly affects wound repair. Gels are ideal for sunburn treatment because they maintain a moist environment, adhere firmly, and do not need to be removed. Herein, we have created bioactive triple-helical recombinant collagen (THRC) gels for improved healing of sunburned skin. The THRC gels remained stable after a three-month stability test, displaying a rheological behavior characteristic of non-Newtonian pseudoplastic fluids. In vivo skin irritation tests conducted on New Zealand rabbits demonstrated that THRC gels were safe for use. A sunburned mice model was established to study the biological effects of THRC gels. Non-invasive combo evaluations indicated that THRC gels exhibited an exceptional capability of recovering dermis density, erythema index (EI), hydration, and transepidermal water loss (TEWL) of sunburned skin to a healthy state. Histological observations revealed that THRC gels significantly enhanced the repair of damaged skin by accelerating the recovery process, promoting collagen deposition and regeneration. Molecular biological characterizations further demonstrated their remarkable antioxidant properties, including the inhibition of lipoperoxidation and the enhancement in superoxide dismutase (SOD) and glutathione (GSH) activities. These safe and bioactive recombinant collagen gels provide a novel approach for sunburn repair and show potential for long-term cosmetic benefits. Full article

This study aimed to evaluate the effects of fruit position, light exposure and fruit surface temperature (FST) on apple fruit colour development and fruit quality at harvest, including sunburn damage severity. This was achieved by undertaking two experiments in a high-density planting of the dark-red apple ANABP 01 in Tatura, Australia. In the 2020–2021 growing season an experiment was conducted to draw relationships between fruit position and fruit quality parameters. Here, sample fruit position and level of light exposure were respectively determined using a static LiDAR system and a portable quantum photosynthetically active radiation (PAR) sensor. At harvest the sample fruit were analysed for percentage red colour coverage, objective colour parameters (L*, a*, b*, hue angle and chroma), sunburn damage, fruit diameter (FD), soluble solids concentration (SSC), flesh firmness (FF) and starch pattern index (SPI). A second experiment was conducted in the 2021–2022 growing season and focused on how fruit shading, light exposure and the removal of ultraviolet (UV) radiation affected the FST, colour development and harvest fruit quality. Five treatments were distributed among sample fruit: fully shaded with aluminium umbrellas, shaded for one month and then exposed to sunlight until harvest, exposed for one month and then shaded until harvest, covered with a longpass UV filter and a control treatment. The development of colour in this dark-red apple cultivar was highly responsive to aspects of fruit position, and the intensity and quality of light exposure. The best-coloured fruit were exposed to higher quantities of PAR, exposed to both PAR and UV radiation simultaneously and located higher in the tree canopy. Fruit that were fully exposed to PAR and achieved better colour development also displayed higher FST and sunburn damage severity. Full article

Nicotinamide adenine dinucleotide (NAD) is one of the most important and essential components within an organism. Extensive ongoing research is aimed at harnessing its potential in managing diverse diseases by supplying various forms of NAD in its oxidized state, NAD⁺. Ultraviolet radiation (UVR) is the most common environmental exposure factor, but also carries many risks. UVR affects the epidermis and contributes to sunburn, photo-allergy, DNA damage, and certain cancers, notably melanoma. Research has shown that NAD⁺ precursors, including nicotinamide riboside (NR), reduce melanogenesis in aged melanocytes. In this study, we used NR to determine whether melanin hyperpigmentation was suppressed after light stimulation. We found that melanogenesis was inhibited when B16F10 cells treated with α-melanocyte-stimulating hormone were exposed to specific doses of NR. Additionally, tyrosinase activity (a key step in melanin production) was suppressed. However, there was no difference in the expression level of melanogenic genes. Ultraviolet B light directly stimulated HaCaT cells, inducing the RNA expression of metalloproteinases. Treatment with NR suppressed the corresponding gene expression and reduced cytotoxicity. This study demonstrates the possibility of using NR as a new skin-whitening ingredient due to its inhibitory effect on hyperpigmentation and ability to maintain skin layers affected by UVR. Full article

Melanoma ranks as the fifth most common solid cancer in adults worldwide and is responsible for a significant proportion of skin-tumor-related deaths. The advent of immune checkpoint inhibition with anti-programmed death protein-1 (PD-1) antibodies has revolutionized the adjuvant treatment of high-risk, completely resected stage III/IV melanoma. However, not all patients benefit equally. Current strategies for improving outcomes involve adjuvant treatment in earlier disease stages (IIB/C) as well as perioperative treatment approaches. Interfering with T-cell exhaustion to counteract cancer immune evasion and the immunogenic nature of melanoma is key for anti-PD-1 effectiveness. Yet, the biological rationale for the efficacy of adjuvant treatment in clinically tumor-free patients remains to be fully elucidated. High-dose intermittent sun exposure (sunburn) is a well-known primary risk factor for melanomagenesis. Also, ultraviolet radiation (UVR)-induced immunosuppression may impair anti-cancer immune surveillance. In this review, we summarize the current knowledge about adjuvant anti-PD-1 blockade, including a characterization of the main cell types most likely responsible for its efficacy. In conclusion, we propose that local and systemic immunosuppression, to some extent UVR-mediated, can be restored by adjuvant anti-PD-1 therapy, consequently boosting anti-melanoma immune surveillance and the elimination of residual melanoma cell clones. Full article

Basal cell carcinoma (BCC) is a slow-growing, locally aggressive, rarely metastasizing, low-grade cutaneous neoplasm that arises from the epidermal basal layer and invades the adjoining tissues. It is the most common skin cancer. It is fairly common in fair Caucasians and quite uncommon in dark-skinned populations. It contributes to 65–75% of cutaneous malignancies in whites and 20–30% in Asian Indians. The most important causal factors appear to be radiation exposure and genetic predisposition. It may present as a nonhealing lesion that occasionally bleeds or as a pruritic lesion with no symptoms. Tumours rarely spread to regional lymph nodes. The clinical appearances and morphology of BCC are diverse. Clinical types include nodular, cystic, superficial, pigmented, morphoeaform, (sclerosing), keratotic and fibroepithelioma of Pinkus. Most of the lesions appear on the head and neck, usually above the line joining the tragus and the angle of the mouth. A biopsy should be performed on all lesions suspected of BCC. The primary aim of treatment is the complete excision of the tumour tissue. Other treatment modalities include cryotherapy, immunomodulatory drugs, laser treatment or locally applicable chemotherapeutic agents. Prevention consists of lifestyle changes such as avoiding sunburn, tanning beds and prolonged direct sun exposure, shade seeking, sunscreen application on the skin, and physical barrier methods such as protective clothing, hats and sunglasses. Regular sunscreen use in childhood and adolescence seems more beneficial than in adulthood. Full article

Skin cancer, a malignant neoplasm originating from skin cell types including keratinocytes, melanocytes, and sweat glands, comprises three primary forms: basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and malignant melanoma (MM). BCC and SCC, while constituting the most prevalent categories of skin cancer, are generally considered less aggressive compared to MM. Notably, MM possesses a greater capacity for invasiveness, enabling infiltration into adjacent tissues and dissemination via both the circulatory and lymphatic systems. Risk factors associated with skin cancer encompass ultraviolet (UV) radiation exposure, fair skin complexion, a history of sunburn incidents, genetic predisposition, immunosuppressive conditions, and exposure to environmental carcinogens. Early detection of skin cancer is of paramount importance to optimize treatment outcomes and preclude the progression of disease, either locally or to distant sites. In pursuit of this objective, numerous computer-aided diagnosis (CAD) systems have been developed. Hyperspectral imaging (HSI), distinguished by its capacity to capture information spanning the electromagnetic spectrum, surpasses conventional RGB imaging, which relies solely on three color channels. Consequently, this study offers a comprehensive exploration of recent CAD investigations pertaining to skin cancer detection and diagnosis utilizing HSI, emphasizing diagnostic performance parameters such as sensitivity and specificity. Full article

For some apple cultivars, inadequate red fruit color development can reduce crop value. The use of reflective groundcovers has been demonstrated to improve red coloration in apples in other regions, but evaluation in the southeastern USA has been limited. To address this, we compared the performance of multiple reflective groundcovers in 2018 and 2020 on mature ‘Fuji’ trees in Edneyville, NC, USA. Woven reflective (Extenday^® DayBright, Lumilys^® WH100, Beltech PD2911, and Belton experimental), mylar, and sod groundcovers were deployed ~5 weeks before anticipated harvest. The effects of the treatment on light reflectance (photosynthetically active and UV radiation), fruit color, fruit quality, and crop value were determined. Across both years of evaluation, reflective groundcovers were consistent in increasing the reflectance of photosynthetically active radiation. However, only Extenday^® DayBright consistently increased reflected UV radiation (250–400 nm), red fruit coloration at commercial harvest, and crop value. Fruit maturity and sunburn incidence were not influenced by any treatment in both years. Reflected UV light quality was not characterized, but it is clear that UV_250–400nm reflectance intensity is critical to enhance ‘Fuji’ fruit color development. Growers in the southeastern US can use reflective groundcovers to enhance red fruit coloration to meet market demands. Full article