Search Results (1,781)

To investigate the molecular characteristics of H3N2 canine influenza viruses circulating in Jiangsu, China, we isolated a H3N2 strain (A/Canine/Nanjing/CnNj01-2025) from a dog presenting with respiratory signs at the Veterinary Teaching Hospital of Nanjing Agricultural University. All eight gene segments were sequenced and compared with those of two human H3N2 strains and five avian H3N2 strains. Antigenicity and receptor-binding properties were also assessed. Phylogenetic analysis revealed that the canine isolate descended from the avian lineage and formed an independent evolutionary clade, while the human strains were more distantly related to the avian lineage. Glycosylation analysis of the HA protein revealed that the canine strain carried seven N-glycosylation sites, including a unique site at residue 97/81 (HA/H3 numbering), which serves as a molecular signature of the canine strain. Several amino-acid substitutions were identified in major antigenic sites, including D97/81N, A176/160T, N204/188D, V212/196I, and W237/222L. Analysis of internal genes showed that the canine strain harbored PB2 292T and 590S mammalian adaptation mutations, which are also present in human strains. Hemagglutination inhibition (HI) assays of the canine strain indicated moderate serologic cross-reactivity with a human H3N2 antiserum (16-fold reduction), whereas avian strains showed no cross-reactivity. Receptor-binding assays demonstrated that the virus retained predominant α-2,3 sialic acid binding, comparable to that of avian influenza viruses, and gained a modest affinity for human-type α-2,6 sialic acid receptors. Therefore, the canine H3N2 virus has undergone significant antigenic drift, developed partial serological cross-reactivity with human strains, and acquired detectable but limited binding affinity for human-type receptors. Overall, our findings suggest that the current canine H3N2 influenza virus exhibits distinct genetic and antigenic variations from human and avian strains. Continuous molecular and serological surveillance of canine influenza viruses is therefore warranted to monitor their evolutionary trends and assess the potential for cross-species transmission. Full article

Objective: To explore the problems with non-National Immunization Program vaccinations in Hubei Province and to provide the basis for follow-up vaccination and management. Methods: Vaccination data on non-NIP/NIP vaccine doses were extracted from the Hubei Provincial Immunization Planning Information Management System. Descriptive epidemiological analyses were conducted to examine dose administration, vaccine-type composition, regional distribution, and substitution patterns. The trend χ² test was used to assess temporal significance. Multistage regression analysis was performed using Joinpoint software. Results: From 2011 to 2024, a total of 91,009,259 doses (annual average: 6,500,661) with 35 types of non-NIP vaccines were administered in Hubei Province, China. The top five vaccines by doses administered were influenza vaccine, rabies vaccine, Hemophilus influenzae type b conjugate vaccine, varicella attenuated live vaccine, and enterovirus 71 inactivated vaccine. Before 2024 (2011–2023), vaccine utilization showed a long-term upward trend: per 10,000, population usage rose from 657.07 (2011) to a peak of 2393.21 (2023) (Increase: 264.22%, χ² = 138.62, p < 0.05) (AAPC = 10.92%, p < 0.05) and non-NIP’s share of total vaccines increased from 25.52% (2011) to 65.95% (2023), (Increase: 154.33%, χ² = 89.47, p < 0.05) (AAPC = 8.74%, p < 0.05). A notable reversal occurred in 2024. Non-NIP doses dropped from 13,971,544 (2023) to 10,238,861 (2024) with population usage falling from 2393.21 (2023) to 1755.03 (2024) (decrease: 26.66%) per 10,000, with the top three declines being in inactivated polio vaccine (IPV) (decrease: 49.53%), influenza vaccine (decrease: 44.21%), and oral rotavirus attenuated live vaccine (decrease: 43.50%). The total number of substitutive non-National Immunization Program (non-NIP) vaccine doses administered reached 16,618,755, with an overall substitution rate of 10.10%. This rate showed a steady upward trend from 5.57% in 2011 to 24.74% in 2023 (trend χ² = 15.11, p < 0.05), yet it increased to 28.03% in 2024. Conclusions: Non-NIP vaccines and NIP-substitute use grew steadily for over a decade, then contracted sharply in 2024. Decision-makers should investigate the sudden dip, differentiate discretionary from replacement demand, and reallocate funds to sustain equity and prevent further erosion of coverage. Full article

In the context of evolving antifungal resistance and increasing reports of clinical outbreaks of non-albicans Candida spp. invasive infections, the rapid detection of resistant patterns is of the utmost importance. Currently, an azole-resistant Candida parapsilosis clinical outbreak is ongoing at Pisa University Hospital. Resistant isolates bear both Y132F and S862C amino acid substitutions. Based on the data and isolates retrieved during the clinical outbreak, mass spectrometry was used to investigate the differences between fluconazole-resistant and -susceptible clinical strains directly from yeast colonies isolated from agar culture media. A total of 39 isolates, 16 susceptible and 23 resistant, were included. Spectra were processed following a standardized pipeline. Several supervised machine learning classifiers such as Random Forest, Light Gradient Boosting Machine, and Support Vector Machine, with and without principal component analysis were implemented to discriminate resistant from susceptible isolates. Support Vector Machine with principal component analysis showed the highest sensitivity in detecting fluconazole resistance (100%). Despite these promising results, external prospective validation of the algorithm with a higher number of clinical isolates retrieved from multiple clinical centers is required. Full article

The emergence and widespread use of low-orbit satellite communication systems has become one of the triggers for the development of the Internet of Vehicles (IoV) technology. The main goal of this integration was to increase the level of vehicle safety not only in cities and their suburbs but especially in remote areas of the country. Despite its effectiveness, satellite IoV remains susceptible to attacks on the radio channel. One of the effective ways to counter such attacks is to use wireless transmission systems with the Frequency-Hopping Spread Spectrum (FHSS) method. The effectiveness of FHSS systems largely depends on the operation of the pseudorandom code generator (PRCG), which is used to calculate the new operating frequency code (number). This generator must have the following properties. Firstly, it must have high cryptographic resistance to guessing a new operating frequency number by an attacker. Secondly, since this generator will be located on board the spacecraft, it must have high fault tolerance. The conducted studies have shown that substitution–permutation network “Kuznechik” (SPNK) meets these requirements. To ensure the property of resilience to failures and malfunctions, it is proposed to implement SPNK in codes of redundant residual class systems in polynomials (RCSP) using the isomorphism of the Chinese Remainder Theorem in polynomials. RCSP codes are an effective means of eliminating computation errors caused by failures and malfunctions. The aim of this work is to increase the fault tolerance of PRCG based on SPNK transformation by using the developed error correction algorithm, which has lower hardware and time costs for implementation compared to the known ones. The comparative analysis showed that the developed algorithm for error correction in RCSP codes provides higher fault tolerance of PRCG compared with other redundancy methods. Unlike the “2 out of 3” method of duplication, the developed algorithm ensures the operational state of PRCG not only when the first failure occurs but also during the subsequent second one. In the event of a third failure, RCSP is able to correct 73% of errors in the informational residues of code combination, while the “2 out of 3” duplication method makes it possible to fend off the consequences of only the first failure. Full article

Gene conversion contributes to gene copy number changes, DNA mutations, and functional innovation and has been widely reported in three domains of life. However, it has hardly been described in Aspergillus, including industrially and commercially important or pathogenic fungi. Here, we revealed multiple sets of homologous genes located in a region of chromosome 1 of A. flavus, and its orthologous counterpart of A. oryzae. Phylogenetic analysis showed evidence of frequent gene (DNA) conversion between ectopic paralogs in each species, accompanied by prominent point mutations and DNA deletion (from several to hundreds of base pairs). At least two independent cases showed that the converted genes in A. oryzae have been repeatedly split into shorter genes by the introduction of stop codons, and then ectopic conversion rendered paralogous genes (regions) to have the same configuration of tandemly located new genes. Inference of nucleotide substitution and ancestral gene content showed that the conversion-affected regions have seen 3.48 times as many substitutions and 4–6 times as many gene losses compared to the non-affected regions. We predicted that a DNA loop between proximal regions, in the common ancestor and inherited by each species, facilitates ectopic gene (DNA) conversion and elevated rates of mutations and losses. Overall, we found that gene conversion proves to be a key factor resulting in genome instability, elevated gene evolutionary rates, and an effective avenue to produce new genes, likely leading to the speciation of two Aspergillus lineages. Full article

In communication environments with limited computing resources, securely and efficiently transmitting image data has become a challenging problem. However, most existing image data protection schemes are based on high-dimensional chaotic systems as key generators, which suffer from issues such as high algorithmic complexity and large computational overhead. To address this, this paper presents new designs for a 1D Sine Fractional Chaotic Map (1D-SFCM) as a random sequence generator and provides mathematical proofs related to the boundedness and fixed points of this model. Furthermore, this paper improves the traditional 2D compressive sensing (2DCS) algorithm by using the newly designed 1D-SFCM map to generate a chaotic measurement matrix, which can effectively enhance the quality of image recovery and reconstruction. Moreover, referring to the principle of gene mutation in biogenetics, this paper designs an image encryption algorithm based on DNA base substitution. Finally, the security of the proposed encryption scheme and the quality of image compression and reconstruction are verified through indicators such as key space, information entropy, and Number of Pixel Change Rate (NPCR). Full article

The tumour suppressor TP53 (tumor protein p53) is a master regulator of cell cycle, DNA repair, and apoptosis, and its mutation is a hallmark of cancer, with individual mutations exerting distinct effects on tumour biology. Despite accounting for ~7% of all TP53 variants, splice site mutations remain the least studied class, and their functional and clinical consequences are poorly understood. We analyzed 25,058 TP53 variants (18,562 somatic; 6496 germline) to characterize the frequency, molecular impact, transcriptional effects, genomic instability, and clinical outcomes of splice mutations. These alterations showed distinct distributions and substitution patterns between germline and somatic contexts and were frequently associated with copy number alterations, reduced TP53 mRNA, and variable protein expression. Transcriptomic profiling identified two transcriptional phenotypes: one with global suppression of canonical p53 target genes and another with mixed activation and repression independent of tumour type. Genomic instability was elevated in a subset of splice-mutant tumours, correlating with increased relapse risk, while other splice mutations showed lower instability but divergent clinical outcomes, including unexpectedly poor prognoses. Our findings fill a critical knowledge gap, defining the biological and clinical spectrum of TP53 splice site mutations and highlighting their potential as prognostic biomarkers and therapeutic targets in precision oncology. Full article

Maize requires substantial nitrogen input, and nitrogen deficiency significantly impairs root development, reducing yield. Therefore, improving maize root system architecture under low-nitrogen (LN) conditions is critical for improving nitrogen use efficiency (NUE). However, the genetic relationship between nitrogen efficiency and root traits is unclear in maize. Here, we conducted a hydroponic experiment during the seedling stage using maize single-segment substitution lines (SSSLs) derived from a cross between the N-efficient inbred line Xu178 and the N-inefficient inbred line Zong3. Quantitative trait loci (QTL) mapping was performed for root architecture traits under both high-nitrogen (HN) and LN conditions. We identified a total of 160 QTLs, with 101 and 59 detected under HN and LN conditions, respectively. These included 19 for root total length (RTL), 43 for root surface area (RSA), 24 for root average diameter (RAD), 60 for root volume (RV), and 14 for root tip number (RTN), distributed across all ten chromosomes, with the highest number on chromosome 1. Additive effects of individual QTLs ranged from −33.14% to 331.16%. Notably, we discovered a major HN-specific QTL cluster on segments end–umc1929 (Bin 7.00) and bnlg1655 (Bin 10.03), and a key LN-specific cluster on segment umc1883–bnlg249 (Bin 6.00). These findings not only highlight distinct genetic bases for nitrogen adaptation at the seedling stage but also provide valuable molecular markers and candidate genomic regions for the marker-assisted breeding of nitrogen-efficient maize varieties. Full article

Mulberry (Morus alba) by-products represent underutilized feed resources with potential for ruminant nutrition. This study evaluated the rumen fermentation kinetics and rumen digestibility of dried mulberry pomace (MP) and leaf (ML) to determine optimal inclusion strategies in dairy cattle diets. Mulberry pomace (MP) and mulberry leaf (ML) were sun-dried and incorporated at 50% substitution levels into total mixed rations (TMR) with varying concentrations (30%, 35%, 40%, 45%, and 50%) of neutral detergent fiber (NDF). This created ten treatment groups: 30NP through 50NP (pomace-supplemented, where the number represents basal TMR NDF%) and 30NL through 50NL (leaf-supplemented), plus control groups containing only MP or ML and five basal TMR controls (30N through 50N). Rumen fluid was collected from two non-lactating Holstein cows fitted with ruminal cannulas. Chemical analysis revealed that ML contained 19% crude protein and 27.4% NDF, while MP contained 14.9% crude protein and 35.8% NDF. The highest gas production was observed in the 45NP (43.20 mL) and 50NL (43.50 mL) groups. Results demonstrated that MP achieved optimal fermentation when combined with 40–45% NDF TMR (maximum total volatile fatty acid (VFA): 88.86 mmol/L in 40NP at 48 h), whereas ML performed best with 45% NDF TMR (45NL: 88.03 mmol/L total VFA), indicating these as the most promising treatment combinations for ruminant feeding systems pending in vivo validation. Acetate proportions were higher in ML groups (84–96%), while propionate ratios were elevated in MP groups. Both materials maintained optimal ruminal pH (6.2–6.8). In vitro NDF digestibility was significantly higher for ML, with differences increasing from 2.97% at 2 h to 16.44% at 240 h. In situ degradation of MP was nearly complete at 48 h, while ML reached maximum degradation at 24 h. These findings indicate the potential of MP and ML as valuable alternative feed sources for ruminants, particularly in TMRs containing 40–45% NDF. Full article

Bioactive glasses remain promising candidates for enhancing osseointegration on metallic implants. However, achieving a composition that combines controlled dissolution, cytocompatibility, and antimicrobial functionality remains an ongoing challenge. Building upon the prior structural and thermal characterization of boron-substituted 6P55 phosphosilicate glasses, this study investigates the biological consequences of incorporating 0, 5, 10, and 15 mol% B₂O₃ to determine their suitability as coatings for Ti6Al4V. Glass extracts were evaluated using L-929 fibroblast cultures (MTT assay and ImageJ-based cell counting), antimicrobial assays against Escherichia coli and Staphylococcus aureus using a semi-quantitative dilution-plating method, and SBF immersion studies to assess pH evolution, surface mineralization, and Ca/P ratio development. FTIR and SEM analyses revealed composition-dependent formation of phosphate-, carbonate-, and silicate-rich surface layers, with 5B exhibiting the most consistent early-stage hydroxyapatite-like signatures, supported by Ca/P ratios approaching the stoichiometric value. The pH measurements showed rapid alkalization for 5B and moderate buffering behavior at higher boron contents, consistent with boron-dependent modifications to network connectivity. Cytocompatibility studies demonstrated a dose- and time-dependent reduction in cell number at elevated B₂O₃ levels, whereas the 0B and 5B extracts maintained higher viability and preserved cell morphology. Antibacterial assays revealed strain-dependent and sub-lethal inhibitory effects, with E. coli exhibiting stronger sensitivity than S. aureus, likely due to differences in cell wall architecture and susceptibility to ionic osmotic microenvironment changes. When considered alongside previously published computational and physicochemical results, the biological data indicate that moderate boron incorporation (5 mol%) provides the most favorable balance between dissolution kinetics, apatite formation, cytocompatibility, and antimicrobial modulation. These findings identify the 5B composition as a strong candidate for further optimization toward bioactive glass coatings on Ti6Al4V implants. Full article

In this article I analyze the symbolic role of the hospital in its social context, from its creation in Rome in the 2nd century BCE to contemporary Montreal hospitals. I trace the change from its original role as a site to isolate the sick to limit the symbolic pollution of the allegedly perfect social body of the Roman state, a trope that became an important vector of unity as Rome expanded and incorporated greater numbers of foreigners and slaves. Today, however, western hospitals have become a semiotic engine where patients construct a new biography to counter the depersonalisation of contemporary medical practices. I propose that today patients use the hospital as raw material to construct a temporal framework that substitutes the rhythms of everyday life that illness and the institutional culture of the hospital have interrupted. These narratives adhere to the same basic structure: the entrance scenario is always admission to the hospital; the plot structure is built with the non-medical details of the daily hospital routine. Surrounded by a neoliberal ethos that insists on the autonomy of the self but silenced by the mechanisation of illness, contemporary patients transform hospitals into semiotic engines where patients use their immediate environment to re-engineer new voices of the self. Full article

In recent years, design education has experienced major changes as the number of digital tools and technologies has rapidly developed. Many design programs encounter difficulties in integrating these innovations, despite their potential benefits. In this research, the adoption of digital tools in the teaching of design in Jordanian universities is explored, focusing on the views of educators in relation to their use, the challenges associated with it, and the resultant effects on the pedagogical process. Faculty members working in various departments of design were surveyed gauging the frequency of usage of tools, their knowledge of new technologies, their perceptions of the potential results of an educational process, and the barriers that were met during the integration process. To guide the analysis, three theoretical frameworks were applied: the SAMR model of technology integration, Bloom’s Digital Taxonomy, and the Technology Acceptance Model (TAM). The findings reveal that while traditional tools like AutoCAD and Revit are predominantly used at the Substitution and Augmentation stages, emerging technologies such as VR/AR and AI show potential for higher-order integration. However, barriers related to ease of use and perceived usefulness limit their broader adoption. The study contributes to the understanding of digital transformation in design education and provides insights into the pedagogical implications for future curriculum development. The research highlights the need to invest more in the professional development of educators and to work more closely with the technological industry. The proposed implications of these insights concern the restructuring of design education to reflect the needs of the digital age and provide approaches to overcoming obstacles to the successful adoption of technology in teaching environments. Full article

This study describes a series of water-soluble half-sandwich ruthenium(II), rhodium(III), and iridium(III) complexes with α-diimine ligands containing substituted aromatic groups. These ligands were derived from glyoxal and 2-aminophenol (a), 4-methyl-2-aminophenol (b), 4-aminophenol (c), phenyl hydrazine (d), and 1-aminonaphthalene (e). The ruthenium(II) (1b–1e), rhodium(III) (2a–2c, 2e), and iridium(III) complexes (3a–3e) were obtained by reacting the ligands (a–e) with the corresponding dimeric precursor [(η⁶-p-cym)RuCl₂]₂ (p-cym = p-cymene) or [(η⁵-Cp*)MCl₂]₂ (Cp* = pentamethylcyclopentadienyl, M = Rh, Ir) in air and under nonanhydro conditions. The air-stable and water-soluble ruthenium(II), rhodium(III), and iridium(III) complexes were characterized via nuclear magnetic resonance spectroscopy and electrospray ionization–mass spectrometry. The structures of complexes [(η⁶-p-cym)Ru(d)Cl]Cl, 1d; [(η⁵-Cp*)Ir(a)Cl]Cl, 3a; and [(η⁵-Cp*)Ir(c)Cl]Cl, 3c were determined via single-crystal X-ray diffraction. Additionally, the complexes exhibited catalytic activity as precatalysts in formic acid decomposition. Complex [(η⁵-Cp*)Ir(d)Cl]Cl, 3d achieved turnover number (TON) and turnover frequency (TOF) values of up to 2150 and 3861 h⁻¹, respectively, at short reaction times. In the hydrogenation of carbon dioxide, [(η⁶-p-cym)Ru(e)Cl]Cl, 1e attained TON and TOF values of up to 1385 and 69.25 h⁻¹, respectively. Full article

This study investigates two sulfonylpurine derivatives, Pur-6-NH₂-SS and Pur-6-Mor-SS, which contain amino and morpholino substituents, for their anticancer potential in 2D and 3D models of human cervical adenocarcinoma (HeLa) cells. Cell cycle distribution, apoptosis, mitochondrial membrane potential, and ROS accumulation were evaluated by flow cytometry. Both Pur-6-NH₂-SS and Pur-6-Mor-SS reduced the proportion of cells in the G0/G1 phase (to 39.65 ± 5.59% and 28.25 ± 1.20%, respectively) when compared with untreated cells. Pur-6-NH₂-SS additionally increased the proportion of cells in the S phase (7.41 ± 0.32%), whereas Pur-6-Mor-SS increased the number of cells in subG0 (21.05 ± 6.15%). Additionally, Pur-6-NH₂-SS triggered early apoptosis in 79.6 ± 8.5% of cells, accompanied by mitochondrial membrane depolarisation in 64.3 ± 9.0%. In comparison, Pur-6-Mor-SS elicited an even stronger apoptotic response, inducing early apoptosis in 87.4 ± 15.6% of cells and mitochondrial membrane potential disruption in 86.8 ± 9.0%, relative to untreated cells. RT-PCR analysis assessed the expression of key regulators, including miR-21, miR-210, and genes involved in survival and stress-response pathways (Akt, CAIX, caspase-3, and cytochrome C). In the 2D model, both derivatives increased CAIX, Akt, and Cyp C expression compared with untreated cells. In contrast, p53 expression remained unchanged in Pur-6-NH₂-SS-treated cells and was slightly decreased in Pur-6-Mor-SS-treated cells. Casp3 expression was slightly elevated following Pur-6-NH₂-SS treatment and remained nearly unchanged in Pur-6-Mor-SS-treated cells. In the 3D model, Pur-6-NH₂-SS exerted a stronger inhibitory effect on CAIX, Akt, p53, Cyp C, and Casp3 expression than Pur-6-Mor-SS, which showed weaker inhibition overall. Both derivatives had a comparable impact on miR-21 and miR-210 expression in 2D and 3D HeLa models. These findings provide mechanistic insight into amino- and morpholino-substituted sulfonylpurine derivatives and highlight how 2D and 3D tumour models influence drug response, offering a basis for further development of purine-based anticancer agents. Full article

The decarbonisation of energy-intensive separation processes is critical for achieving net-zero goals in the chemical industry. While widely used for separating azeotropic mixtures, pressure swing distillation (PSD) remains highly energy-intensive due to significant thermal demands. This work presents a comprehensive systems-based assessment of electrified distillation designs, with a specific focus on tetrahydrofuran–water separation as a case study. Using Aspen Plus and Aspen Plus Dynamics, key performance indicators, including controllability, thermal and exergy efficiencies, and CO₂ emissions reduction potential, are evaluated. The electrified configurations employed heat pumps as substitutes for conventional steam heating. Disturbance rejection was applied to compare the input–output pairings and select pairings with the best controllability and disturbance rejection indices. Results showed that the conventional PSD (CPSD) exhibited higher Morari Resiliency Index (MRI) and acceptable Condition Number (CN) values, indicating better robustness and disturbance rejection than the heat pump-assisted PSD (HPAPSD). Despite this, HPAPSD achieved a 59% reduction in primary energy demand, a 23% increase in exergy efficiency, and an 82% reduction in CO₂ emissions. This study demonstrates the potential of electrification to transform PSD systems from rigid, energy-intensive operations into flexible and sustainable processes. The findings support a shift towards integrated, systems-driven design strategies in chemical separation, aligning with broader goals in process electrification, circularity, and net-zero manufacturing. Full article