Search Results (183)

Since 2011, massive strandings of pelagic Sargassum have become a recurrent environmental hazard across the tropical Atlantic and Caribbean archipelago, creating an urgent need for reliable short-term drift forecasts to support coastal risk management. This study evaluates key sources of uncertainty in operational Sargassum drift forecasting by analyzing the sensitivity of Lagrangian simulations to the representation of floating material and to environmental forcing fields. The analysis uses two complementary observational datasets: trajectories of four GPS-tracked Sargassum mats deployed near Puerto Rico and thirteen 24 h displacement vectors derived from sequential Sentinel-3 satellite detections across the tropical North Atlantic. Drift simulations were performed with the MOTHY model under multiple configurations, testing two material parameterizations, different atmospheric forcings, and several ocean circulation products and vertical current integration strategies. The results indicate that the best agreement with observed trajectories is obtained for partially immersed structures, highlighting the importance of balancing wind exposure and hydrodynamic drag. Sensitivity experiments further show that ocean circulation forcing dominates trajectory skill, while higher-resolution atmospheric forcing provides limited improvement under offshore conditions. Overall, the study confirms the importance of accurately representing upper-ocean transport processes and provides observational support for several operational choices implemented in the Météo-France Sargassum forecasting system. Full article

Although the long-term behavior of ground anchors depends fundamentally on interfacial behavior, the independent effect of the strand–grout interface on load loss has not been comprehensively investigated. This study establishes a physical model testing method that isolates the strand–grout interface and systematically investigates both short-term and long-term load loss behavior. Pull-out tests and long-term monitoring tests were conducted using grout uniaxial compressive strength ($q_u$ = 18–30 MPa) and bond length ($L_b$ = 900–1500 mm) as primary design variables. Long-term monitoring confirmed that prestress loss at the strand–grout interface is induced by the progressive pull-out displacement of the strand over time, following a logarithmic decay pattern. The load reduction coefficient n was significantly more sensitive to $L_b$ than to $q_u$; n increased sharply from 0.015 to 0.069 as $L_b$ decreased. Anchors with insufficient bond length exhibited secondary load reduction behavior that disrupted the stable log-linear decay, posing significant risk to long-term performance. Based on RMSE analysis of the fitted logarithmic model, a minimum monitoring period of approximately 50 days is recommended for reliable long-term prediction when bond length is adequate. These findings identify $q_u$ and $L_b$ as the governing parameters, providing a quantitative basis for optimizing prestress design and enhancing the long-term reliability of anchor systems. Full article

With the rapid expansion of urban underground space in China, anti-floating has become a critical challenge, and uplift piles are a key solution. Previous studies on composite anchor-cable uplift piles have primarily focused on small-diameter single-pipe types (≤600 mm), often simplifying the pile as an integral component, leaving the multi-interface stress transfer mechanisms of large-diameter piles inadequately understood. This study proposes a back-analysis method based on orthogonal experiments, implemented using Abaqus 3D finite element software, to determine interfacial mechanical parameters for three critical contact pairs (strand-grout, grout-steel pipe, steel pipe-concrete) in large-diameter multi-pipe composite anchor-cable uplift piles. These parameters are then implemented in a refined 3D finite element model to simulate the load-deformation behavior of such piles. Quantitative results show that the back-calculated parameters are highly reliable, with maximum simulation errors for pile head displacement limited to 13.0% and 9.6% for fully bonded and semi-bonded piles, respectively. Unlike conventional piles, stress and strain in this new pile type transfer progressively from the inner steel strands outward and from the top downward, resulting in reduced pile-soil displacement mismatch, fuller mobilization of side interfacial strength, and effective mitigation of concrete cracking. This study provides a systematic parameter-calibration framework and numerical platform, offering theoretical and technical support for optimized design and engineering application of large-diameter composite uplift piles. Full article

Four-point bending tests were conducted on precast ultra-high-performance concrete (UHPC) segmental beams reinforced with unbonded prestressing tendons. A nonlinear finite element model was established and rigorously validated against the experimental data to simulate their flexural behavior. The experimental results show that compared with monolithic beams, the segmental beams experience a slight reduction in flexural capacity of 9.22% and 12.44% for the double-joint and triple-joint configurations, respectively. Nevertheless, the segmental beams possess greater ductility reserves; specifically, their average peak displacements increased from 9.83 mm for the monolithic beams to 11.60 mm and 14.78 mm for the double-joint and triple-joint beams, respectively, demonstrating substantially improved ductility. Based on the validated finite element model, extensive parametric analyses were performed. The numerical results indicate that concrete strength and steel strand reinforcement ratio significantly enhance the load-carrying capacity. Furthermore, shifting the joint positions away from the loading points increases the beam’s bending capacity, though this enhancement aggressively flattens out beyond a critical distance threshold of 0.25 L (L is the effective span). Finally, segmental beams with shear-resistant keyed joints exhibit higher overall stiffness and ultimate load-carrying capacity compared to those with plain flat joints. Full article

Gentrification refers to a transformation in the composition of land users whereby in-coming users possess a higher socio-economic status than those they replace, accompanied by reinvestment in the built environment and the physical transformation of urban space. Displacement is an essential part of this process. Gentrification has become one of the central analytical concepts in urban studies. Gentrification has become one of the central analytical concepts in urban studies enabling the analysis of socio-spatial restructuring processes in cities and has been applied to a broad range of geographical settings and historical conditions. Originally coined in the context of post-war London, the concept has since traveled widely and has been applied to a broad range of geographical settings and historical conditions. This entry provides a comprehensive overview of the evolution of the concept, its principal theoretical interpretations, and its empirical applications. It reviews the major strands of explanation—demand-side, supply-side, and institutionalist approaches—and situates them within broader debates in urban theory. Particular attention is devoted to the relationship between gentrification and displacement, including both classical conceptualizations and recent efforts to capture its more diffuse and subjective dimensions. The entry concludes by arguing that while gentrification remains a key concept for analyzing urban change, it must be continuously reworked in light of emerging dynamics such as financialization, digitalization, and trans-local housing practices. It calls for more systematic and genuinely comparative research in order to better understand the evolving geographies of gentrification. Full article

Na⁺,K⁺-ATPase possesses a highly conserved glycine (G358 in the α3 isoform) that—together with a nearby isoleucine (I363 in α3)—is targeted by mutations causing some of the most severe neurological phenotypes of the clinical spectrum of α3-Na⁺,K⁺-ATPase mutations. The disease mutations α3-G358V and α3-I363N affect Na⁺ and K⁺ transport to an extent incompatible with cell growth. However, alanine replacement of the corresponding glycine G363 in the α1 isoform is compatible with cell growth, allowing the effects on Na⁺,K⁺-ATPase function to be addressed using enzymatic assays on plasma membranes isolated from transfected cells. Occlusion of Na⁺ appears to be defective in mutant G363A, resulting in a reduced rate of phosphorylation from ATP. Furthermore, the mutation displaces the major conformational equilibrium of Na⁺,K⁺-ATPase such that the K⁺-occluded state is destabilized and occluded K⁺ is released faster, thereby leading to accumulation of a non-productive state without bound Na⁺ or K⁺. The critical function of the glycine can be ascribed to a strategic location at the bending point between an α helix and a β strand, where it connects the catalytic ATP hydrolysis site in the cytoplasmic P domain with the ion-binding region in the membrane and coordinates important intramolecular domain movements during the Na⁺,K⁺-ATPase transport cycle. Full article

Ochratoxin A (OTA) is a highly toxic mycotoxin commonly detected in food and agricultural products, requiring sensitive analytical methods for reliable monitoring. Herein, we report an ultrasensitive turn-on electrochemical aptasensor for OTA detection based on a target-induced displacement of an aptamer–complementary DNA (cDNA) duplex assembled on an electrochemically reduced graphene oxide (ERGO)-modified glassy carbon electrode (GCE). In the absence of OTA, a methylene blue (MB)-labeled aptamer hybridized with cDNA is immobilized on the ERGO surface via π–π stacking interactions, forming a rigid duplex that suppresses electron transfer and yields a low electrochemical signal. Upon OTA binding, the aptamer undergoes a conformational transition into a G-quadruplex structure, leading to dissociation of the cDNA strand. This target-induced folding brings the MB redox tag into close proximity to the ERGO surface, markedly accelerating electron transfer and enhancing the cathodic reduction current of MB, thereby producing a pronounced signal-on response in square-wave voltammetry (SWV). The ERGO-modified electrode provides a conductive and stable interface without chemical linkers. Under optimized conditions, the aptasensor shows a linear response to OTA from 10 fM to 100 pM with an ultralow LOD of 0.67 fM, together with high selectivity, good reproducibility, and satisfactory stability. This work demonstrates a simple and effective turn-on aptasensing strategy for sensitive electrochemical detection of OTA. Full article

MicroRNAs, pivotal regulators of gene expression and physiology, serve as reliable biomarkers for early cancer diagnosis and therapy. As one of the earliest discovered miRNAs in the human genome, miRNA-21 provides critical information for early cancer diagnosis, drug therapy, and prognosis. In this work, we harness CRISPR as a bridge to integrate target-induced self-priming hairpin isothermal amplification (SIAM) with terminal transferase (TdT) polymerization labeling, constructing a facile, straightforward electrochemical biosensor for sensitive miRNA-21 detection. Unlike conventional single-strand template-based exponential amplification (EXPAR), the SIAM hairpin undergoes target triggered intramolecular conformational change, initiating extension and strand displacement reactions that suppress nonspecific dimer formation and lower background current. Notably, the assay requires only a single probe, enabling unidirectional signal amplification while nonspecific reactions caused by system complexity. The generated SIAM products activate the Cas12a/crRNA complex to trans-cleave PO₄³⁻ modified single-stranded DNAs (ssDNAs); the resulting 3′ hydroxyl ssDNAs are subsequently labeled by TdT, with the assistance of SA-HRP catalyzing hydrogen peroxide, achieving robust signal amplification. Under optimized conditions, the cathodic current exhibits a logarithmic relationship with miRNA concentrations from 20 fM to 5.0 × 10⁸ fM, with a detection limit of 9.2 fM. The biosensor successfully quantified miRNA-21 in commercial serum samples and biological lysates, demonstrating its potential for cancer diagnostics and therapy. Full article

Transcription–replication conflicts (TRCs) often occur in cells and cause DNA replication fork stalling. In this study, we investigated the interplay of RNA polymerase (RNAP), DNA polymerase, and RNase H1 (RH1) during head-on TRC in vitro with precise control over the reaction conditions. We show that it is a catalytically competent transcription elongation complex (TEC) that interferes with the action of both the Klenow fragment and full-length DNA Pol I. An incompetent RNAP complex with an R-loop stimulates the 3′→5′ exonuclease activity and pauses the DNA polymerase during head-on TRC. As RNAP advances along the DNA template, elongating the RNA, the head-on TRC is slowly overcome in our model system, likely through the reassociation of the displaced DNA polymerase with the nontemplate DNA strand upstream of RNAP. An isolated R-loop containing an 11-nt heteroduplex (R-loop-11) does not interfere with DNA replication by the Klenow fragment. For DNA Pol I, such an R-loop also does not stall replication but stimulates its 3′→5′ exonuclease activity. We demonstrate that a stalled Klenow fragment does not interfere with transcription, whereas a Klenow fragment moving along the TRC substrate towards RNAP alters the kinetics of RNAP. Stalled DNA Pol I does not stop RNAP but stimulates its endonuclease activity. We find that RH1 alone does not displace stalled RNAP from a competent TEC containing R-loop-11 and does not resolve the head-on TRC. On the other hand, RH1 displaces RNAP from the incompetent complex with the TRC substrate. This eliminates the stimulation of the 3′→5′ exonuclease activity of DNA polymerase during head-on TRC. Full article

The current study investigates the influence of timber-to-concrete connection types on the behaviour of timber–concrete composite (TCC) structures employing metal web timber joists. Two groups of laboratory specimens were prepared, each comprising four samples with push-joisted beams joined by oriented strand board (OSB) and cast with a concrete layer. One group utilised compliant timber-to-concrete connections via perforated steel tape angles, while the other employed rigid connections through epoxy adhesive and granite chips. The specimens, consisting of two 1390 mm long beams of grade PS10 timber, were tested under three-point bending. Experimental results and finite element analyses demonstrated that specimens with compliant connections exhibited 14–16% greater maximum vertical displacements but only a marginal 1.79% reduction in load-carrying capacity compared to those with rigid connections. Findings indicate that connection compliance markedly affects stiffness and deflection but has a minor impact on ultimate strength. These insights can guide optimisation of TCC members with metal web joists, balancing structural performance and design requirements in sustainable timber construction. Full article

The development of wind power aligns with the strategy of low-carbon development and plays a crucial role in the global transition to a green economy. The segmented steel–concrete wind turbine tower offers advantages such as modular fragment prefabrication, prestressed structural enhancement, and integrated intelligent construction. To investigate the structural performance of such towers, this paper established a numerical model based on an existing project. The model was validated against previous experiments and used for parametric analysis. A numerical model of a segmented steel–concrete wind turbine tower was developed to evaluate its overall deformation, stress distribution, and vertical and horizontal joint separation under various conditions. The concrete segment of the tower was numerically simplified, and a comparative analysis of structural performance was conducted between the detailed and simplified models. Based on the simplified model, the effects of the friction coefficient, prestress loss, and contact area on the anti-slip performance of the transition section of the towers were investigated and analyzed. The results indicated that the validity of the modeling approach was confirmed through the existing experimental results. The top displacement of the model incorporating vertical and horizontal joints (Model 1) did not exceed the limit of 1/100 under the safety factor considerations, indicating that the structure could ensure safety. The simplified model (Model 2) showed consistent behavior with Model 1, thereby providing a reliable basis for parametric studies. A reduction in the steel-to-steel friction coefficient, steel strand prestress, and contact area between the steel transition section and the embedded anchor plate resulted in an increase in the horizontal relative displacement between the steel transition section and the embedded anchor plate to varying extents. Notably, a more pronounced increase in displacement was observed under higher loading conditions. Overall, the horizontal relative displacement between the steel transition section and embedded anchor plate under single-loading conditions was below one millimeter in most of the studied conditions, which was relatively small compared to the assembly tolerance of the structure. Full article

Nucleic acid strand displacement reactions (SDRs) are fundamental building blocks of dynamic DNA nanotechnology. A detailed understanding of their kinetics is crucial for designing efficient sequences and regulating reaction networks with applications in biosensing, synthetic biology, biocomputing, and medical diagnostics. Since the development of toehold-mediated strand displacement, researchers have devised many strategies to adjust reaction kinetics. These efforts have expanded the available tools in DNA nanotechnology. This review summarizes the basic principles and recent advances in activation strategies, emphasizing the role of strand proximity as a central driving force. Proximity-based approaches include toehold docking, associative toeholds, remote toeholds, and allosteric designs, as well as strategies that operate without explicit toehold motifs. These methods enable flexible and scalable construction of DNA reaction networks. We further discuss how combining different activation and kinetic control approaches gives rise to dynamic networks with complex and dissipative behaviors, providing new directions for DNA-based nanotechnology. Full article

Nucleic acid amplification methods are widely used in science, medicine and forensics for molecular biological assays and for the detection of genetic material. The newly developed strand displacement chain reaction (SDCR) method is a hybrid amplification technique based on polymerase chain reaction (PCR) and isothermal nucleic acid amplification. Here, we compared conventional PCR, the “gold standard” for molecular diagnostic assays, with the SDCR method by performing real-time amplification assays using human, bacterial and viral genetic materials. In the assays, SDCR demonstrated very high sensitivity and amplification efficiency. We found that the SDCR method provided an amplification factor above three, which noticeably outperformed that of PCR amplification and enabled a marked reduction in the number of cycles in comparison with PCR. Therefore, the new hybrid amplification technique could be extremely useful for the detection of genetic material and the development of new diagnostic kits. Full article

Schiff base ligands prepared from salicylaldehyde and 2-, 3- and 4-methoxybenzylamine were used to prepare copper(II) complexes, characterized by spectral methods, elemental analysis and X-ray crystallography in the case of complex 4a derived from 2-methoxybenzylamine. The DNA cleavage activity of the prepared complexes was exceptional, with best activities of over 95% one-strand cleavage for 4c at 3 mM and full double-strand cleavage for complex 4a at 5 mM. Absorption titration studies with ct-DNA revealed good binding constants (at 10⁵ M⁻¹) with a decrease of up to 56% light absorption. Meanwhile, the EB–DNA displacement method and viscosity studies revealed groove binding as a possible binding mode. For BSA binding studies, all three complexes showed K_BSA values in the optimal range for reversible BSA binding (10⁴ M⁻¹). The copper(II) complexes showed significant cytotoxic effects (67–96% at 1 mM) in mitochondrial activity monitoring assays. Cytotoxicity was confirmed against cancer cell lines (A549 and HepG2) and HEL cells. The complexes 4a and 4c exhibited high activity against HepG2 cancer cells (IC50 < 22 μM), comparable to cisplatin. The radical scavenging activity was determined by the INT method with the best IC50 for 4c (189 ± 11 μM). Overall, complexes 4a and 4c with a methoxy group in the ortho and para positions show high potential in most determined activities, but mainly as DNA cleavers and as cytotoxic agents with selectivity against HepG2 cells. Full article

Polymerase-coupled nanopore sequencing requires DNA polymerases with strong strand displacement activity and high processivity to sustain continuous signal generation. In this study, we characterized two novel B family DNA polymerases, SRHS and BBum, isolated from Bacillus phages SRT01hs and BeachBum, respectively. Both enzymes exhibited robust strand displacement, 3′→5′ exonuclease activity, and maintained processivity under diverse reaction conditions, including across a broad temperature range (10–45 °C) and in the presence of multiple divalent metal cofactors (Mg²⁺, Mn²⁺, Fe²⁺), comparable to the well-characterized Phi29 polymerase. Through biochemical analysis of mutants designed using AlphaFold3-predicted structural models, we identified key residues (G96, M97, D486 in SRHS; S97, M98, A493 in BBum) that modulated exonuclease activity, substrate specificity and metal ion utilization. Engineered variants SRHS_F and BBum_Pro_L efficiently incorporated unnatural nucleotides in the presence of Mg²⁺—a function not observed in Phi29 and other wild-type strand-displacing B family polymerases. These combined biochemical features highlight SRHS and BBum as promising enzymatic scaffolds for nanopore-based long-read sequencing platforms. Full article