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Application of Electrochemical Materials in Molecular Biology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Materials Science".

Deadline for manuscript submissions: 30 October 2025 | Viewed by 886

Special Issue Editor


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Guest Editor
The Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland
Interests: nucleic acids and antibodies; biosensor sensing layers; molecular recognition; the construction and analysis of receptor layers of affinity biosensors (nucleic acids, including aptamers and antibodies); the construction of ready-made sensor solutions, substrates manufactured using printed electronics technology
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Special Issue Information

Dear Colleagues,

It has been said that “Molecular Electrochemistry” can be defined as “molecules for electrochemistry and electrochemistry for molecules”. In the era of almost unlimited possibilities in manufacturing materials of desired and carefully adjusted properties and an omnipresent tendency to downsize (bio)analytical devices, electrochemistry takes on new meaning. Considering such an approach, the electrode material not only plays the role of electron reservoir or sink but also the transformation of molecules triggered by electron transfer from or to an electrode and/or using molecules dispersed in the solution or attached to the electrode surface to accelerate and optimize an electrochemical reaction, makes capable of gaining in importance. As such, a mechanism could take place on a nanometer scale but with significant influence on the overall readout signal, making it an ideal candidate for detection element in any portable devices, especially when dedicated to molecular diagnostics in the POC mode.

This Special Issue of IJMS aims to focus on the application of electrochemical materials in molecular biology. We welcome submissions of research articles, comprehensive reviews, etc.

Dr. Robert Ziółkowski
Guest Editor

Manuscript Submission Information

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Keywords

  • electrochemistry
  • (bio)analytical chemistry
  • molecular biology
  • point-of-care
  • nanomaterials
  • (bio)molecules

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Published Papers (1 paper)

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Review

17 pages, 2003 KiB  
Review
Recent Advances in the Electrochemical Biosensing of DNA Methylation
by Sanu K. Anand and Robert Ziółkowski
Int. J. Mol. Sci. 2025, 26(13), 6505; https://doi.org/10.3390/ijms26136505 - 6 Jul 2025
Viewed by 454
Abstract
DNA methylation, as a critical epigenetic modification, plays a central role in gene regulation and has emerged as a powerful biomarker for early disease diagnostics, particularly in cancer. Owing to the limitations of traditional bisulfite sequencing—such as high cost, complexity, and chemical degradation—electrochemical [...] Read more.
DNA methylation, as a critical epigenetic modification, plays a central role in gene regulation and has emerged as a powerful biomarker for early disease diagnostics, particularly in cancer. Owing to the limitations of traditional bisulfite sequencing—such as high cost, complexity, and chemical degradation—electrochemical biosensors have gained substantial attention as promising alternatives. This review summarizes recent advancements in electrochemical platforms for bisulfite-free detection of DNA methylation, encompassing direct oxidation strategies, enzyme-assisted recognition (e.g., restriction endonucleases and methyltransferases), immunoaffinity-based methods, and a variety of signal amplification techniques such as rolling circle amplification and catalytic hairpin assembly. Additional approaches, including strand displacement, magnetic enrichment, and adsorption-based detection, are also discussed. These systems demonstrate exceptional sensitivity, often down to the attomolar or femtomolar level, as well as high selectivity, reproducibility, and suitability for real biological matrices. The integration of nanomaterials and redox-active probes further enhances analytical performance. Importantly, many of these biosensing platforms have been validated using clinical samples, reinforcing their translational relevance. The review concludes by outlining current challenges and future directions, emphasizing the potential of electrochemical biosensors as scalable, cost-effective, and minimally invasive tools for real-time epigenetic monitoring and early-stage disease diagnostics. Full article
(This article belongs to the Special Issue Application of Electrochemical Materials in Molecular Biology)
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