Fluorescent Probes for Bioimaging and Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (1 June 2025) | Viewed by 422

Special Issue Editors


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Guest Editor
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, China
Interests: bioanalytical chemistry; chemical biology
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, China
Interests: biosensing; theranostic nanomedicine; medicinal chemical biology; targeted nanocarriers

E-Mail Website
Guest Editor
School of Rehabilitation Sciences and Engineering, University of Health and Rehabilitation Sciences, Qingdao 266071, China
Interests: chemical biology; medicinal chemistry; mass spectrometry and proteomics

Special Issue Information

Dear Colleagues, 

The development of fluorescent probes in the field of bioimaging and biosensors is a cutting-edge area of multidisciplinary intersection and rapid technological advancement. Fluorescent probes have become an indispensable tool for bioimaging due to their wide range of applications in biomass detection, cellular imaging, in vivo biochemical reaction process tracking, and disease biomarker monitoring. Compared with traditional bioimaging techniques, fluorescent probes have the advantages of real-time imaging, in-depth visualization, and low damage to biological samples, making them increasingly popular. In this Special Issue, we will focus on the latest research progress, innovative applications, and future trends in the field of fluorescent probes in bioimaging and biosensors, including protein-mediated fluorescent probes, organic fluorescent probes, fluorescent biosensors, near-infrared two-region fluorescent probes, genetically encoded fluorescent sensors, etc. Moreover, this Special Issue will also focus on interdisciplinary collaborations and performance optimization of probes with the aim of providing researchers with a platform for exchanging and sharing the latest research results and promoting scientific developments in this field.

Dr. Yan Zhao
Dr. Weiheng Kong
Dr. Shengyan Yin
Guest Editors

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Keywords

  • bioimaging
  • fluorescent probes
  • biosensors
  • near-infrared two-region fluorescence
  • molecular diagnostics

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Published Papers (1 paper)

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Research

16 pages, 6714 KiB  
Article
Construction of Graphene Oxide Probes Loaded with Antisense Peptide Nucleic Acid and Doxorubicin for Regulating Telomerase Activity and Inducing Apoptosis of Cancer Cells
by Yanyan Zhu, Qinghong Ji and Min Hong
Biosensors 2025, 15(6), 337; https://doi.org/10.3390/bios15060337 - 26 May 2025
Viewed by 227
Abstract
In this study, we developed a multifunctional graphene oxide (GO)-based nanoprobe co-loaded with antisense peptide nucleic acid (PNA) and the chemotherapeutic agent doxorubicin (DOX). The nanoplatform was strategically functionalized with folic acid ligands to enable folate receptor-mediated tumor targeting. Upon cellular internalization, the [...] Read more.
In this study, we developed a multifunctional graphene oxide (GO)-based nanoprobe co-loaded with antisense peptide nucleic acid (PNA) and the chemotherapeutic agent doxorubicin (DOX). The nanoplatform was strategically functionalized with folic acid ligands to enable folate receptor-mediated tumor targeting. Upon cellular internalization, the antisense PNA component selectively hybridized with human telomerase reverse transcriptase (hTERT) mRNA through sequence-specific recognition, inducing structural detachment from the GO surface. This displacement restored the fluorescence signal of previously quenched fluorophores conjugated to the PNA strand, thereby enabling the real-time in situ detection and quantitative fluorescence imaging of intracellular hTERT mRNA dynamics. The antisense PNA component effectively reduced the hTERT mRNA level and downregulated telomerase activity via an antisense gene regulation pathway, while the pH-responsive release of DOX induced potent cancer cell apoptosis through chemotherapeutic action. This combinatorial therapeutic strategy demonstrated enhanced anticancer efficacy compared to single-modality treatments, achieving a 60% apoptosis induction in HeLa cells through coordinated gene silencing and chemotherapy. This study establishes GO as a promising dual-drug nanocarrier platform for developing next-generation theranostic systems that integrate molecular diagnostics with multimodal cancer therapy. Full article
(This article belongs to the Special Issue Fluorescent Probes for Bioimaging and Biosensors)
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