Search Results (149)

Land maxing in cultivated ecosystems can improve upon other agroecological approaches because in this approach social, economic and ecological benefits are maximized within the available land, in part through the careful selection of plant species with specific benefits, e.g., biodiversity conservation, provision of ecological services, diversifying and improving farmer incomes. In this approach, plant species selected for improving farmer incomes are those providing non-timber marketable products, and plant species selected for biodiversity conservation and provision of ecological services can be identified quantitatively via ecological network theory. Here, we demonstrate using ecological network theory to identify (a) farm management practices associated with ecological network indices, and (b) key plant species that farmers can plant to maximize the potential for their land to support bees and pollination services. In this study we quantified bee-plant interaction networks within 10 agroforests, and compared results between the entire bee community and the subsetted stingless bee community. Bee abundance increased with flowering plant richness, explaining 9% of the variance (R² = 0.09; β = 0.05, SE = 0.03). Diverse agroforests with higher numbers of tree species supported less connected (R² = 0.67; β = −0.08, SE = 0.02), less nested (R² = 0.53; β = −0.05, SE = 0.01), and more specialized (R² = 0.63; β = 0.07, SE = 0.02) and modular (R² = 0.37; β = 0.05, SE = 0.02) bee-plant networks. Some key plant species with the strongest impacts on network structure and stability were shared between the entire bee-plant and the stingless bee-plant networks. We recommend that farmers plant the species highlighted in this study to maximize the value of their diverse agroforests to support bee communities and pollination services. Full article

Propolis is an apicultural product known for its antioxidant, antimicrobial and anti-inflammatory properties. However, its composition varies with botanical sources, geography, season and bee species, complicating quality control and creating opportunities for adulteration, such as the addition of poplar bud extracts or non-propolis resins. This review synthesizes the latest primary studies and reviews addressing chemical markers identified through analytical platforms, such as TLC, HPTLC, HPLC, LC-MS, GC-MS, NMR, FTIR and ICP, often integrated with chemometrics and machine learning for authentication and standardization. Marker panels are linked to regional chemotypes, including poplar-type, Brazilian green, red and brown, Cuban variants, and stingless bee propolis. Fraud detection strategies using marker-based screening and spectral pattern recognition are also summarized. Multi-marker and chemometric approaches consistently differentiate botanical types, origins and commercial extracts. Common marker families include flavonoids (pinocembrin, chrysin, galangin), phenolic esters (CAPE, benzyl/allyl caffeates), prenylated cinnamates like artepillin C, lignans, and volatile terpenoids or benzenoids. Rapid screening by ATR-FTIR and NMR is often complemented with LC-MS for confirmatory quantitation. Propolis quality control is moving toward harmonized workflows combining FTIR/NMR/HPTLC screening with LC-MS verification and optional elemental or volatile profiling, paving the way for shared marker sets and international standards similar to those for honey. Full article

This perspective paper examines the multifaceted cultural ecosystem services (CESs) provided by wild bees with particular attention to the spiritual, medicinal, and traditional knowledge-based dimensions associated with Afrotropical stingless bees. We integrate these insights within a biocultural framework, highlighting their relational values and arguing that the systematic omission of these cultural roles in pollinator research, policy and conservation constitutes a form of epistemic injustice. We also argue that the systematic recognition and documentation of these cultural roles are critical to advancing more inclusive and effective conservation frameworks. Based on a synthesis of field observations, photo-documentation, and literature review, we propose future research and policy directions aimed at embedding these cultural values more comprehensively within biocultural conservation strategies. Full article

Domestic demand for honey in Kenya consistently exceeds national production, resulting in periodic reliance on imports. Kenyan honey is typically branded and marketed according to its geographical origin, whereas information regarding botanical origin is rarely communicated. This study was undertaken in two phases: a systematic review of the literature on honey varieties in Kenya—with an emphasis on legislation, production, and processing—and an online survey assessing front-of-pack (FoP) labeling descriptions. Legislatively, Kenyan honey varieties are categorized based on (i) the bee species producing the honey (honeybee or stingless bee), (ii) the intended use (direct human consumption or industrial application), and (iii) the presence of added flavoring agents. The results from the FoP labeling survey indicated that all domestic honey samples (n = 24) failed to comply with labeling requirements, instead emphasizing descriptors such as “natural” and “pure.” Only 40% of imported honey brands (n = 10) declared the botanical origin and processing method. Mellisopalynological studies showed that honey produced in the Acacia woodlands of Baringo, West Pokot, and Kitui can legitimately be marketed as Acacia honey. In contrast, honey from the Eastern Mau forest can be characterized as monofloral Eucalyptus, Croton, Albizia, or Cordia spp. honeys, with numerous bifloral and multifloral combinations. Sisal and mangrove honeys were also identifiable in landscapes dominated by these plant species. The lack of legislative classification for Kenyan monofloral honeys appears to contribute to widespread non-compliance in industry labeling practices. Although Kenyan honey remains competitive, inadequate product differentiation and weak labeling hinder access to niche domestic and international markets. To strengthen competitiveness, Kenyan honey legislation should incorporate provisions for characterizing monofloral honey types, processing standards, and mellisopalynological authentication. Such measures will enhance producer awareness, promote adoption of good processing practices, strengthen compliance with trade regulations, and support the development of a robust national honey value chain. Full article

Bee declines are making headlines, yet awareness of native bees is limited, which may stem in part from poor taxonomic knowledge and under-representation in media and education. This study aimed to determine the Australian public’s taxonomic knowledge of native bees and identify the relative popularity of species. We explored this by asking “What is your favourite Australian bee species?” to members joining the Facebook group “The Buzz on Wild Bees”. Over nine months, 580 people responded, but only 400 provided an answer of their favourite Australian native bee species, with 419 answers in total (as some provided more than 1 answer). Of these answers, just 12.9% provided a name for a species, rather than a group, and only 7.6% provided a scientific name. The group that emerged as the favourite were the Amegilla (“blue banded” and “teddy bear” bees), followed by the Meliponini (“sugarbag” or “stingless” bees). Euryglossinae, Neopasiphaeinae, and Stenotritidae were absent from the answers. We discuss our results in light of the taxonomic literacy among the public, and hypothesise why certain taxa are more or less popular. Amegilla species could represent a good gateway into raising awareness among the general Australian public about native bees. The selection of region-specific, vulnerable species such as Xylocopa aerata may better serve as flagships. Greater public exposure and education of under-represented Australian native bee taxa is required. Full article

The stingless bee Meliplebeia beccarii, endemic to Ethiopia, plays a crucial ecological and economic role through pollination and high-quality honey production. However, habitat degradation and anthropogenic pressures threaten its survival. In this study, we present the complete mitochondrial genome (mitogenome) of M. beccarii, revealing a compact structure of 15,458 bp with 13 protein-coding genes (PCGs), 19 tRNAs, and two rRNAs, characterized by an A + T bias (83.9%). Unique features include the absence of trnI, trnK, and trnA, translocation of trnQ and a novel inversion in the trnT-trnP combination. These findings highlight species-specific genomic adaptations. Phylogenetic analysis based on concatenated PCGs places M. beccarii within the Apidae lineage, contributing to a deeper understanding of stingless bee evolution. Our results underscore the utility of mitogenomic studies in biodiversity conservation and evolutionary biology, providing foundational insights for the management and preservation of M. beccarii. Full article

Stingless bees, particularly Tetragonula pagdeni, are vital for both ecosystems and the economy due to their pollination services and nest products. However, little is known about their nesting habits. This study investigated the nesting ecology of Tetragonula pagdeni in West Bengal, India. The species was found inhabiting a variety of landscapes, including agricultural, forest, rural, semi-urban, and urban areas, with a greater abundance in rural areas featuring mixed vegetation. Colonies, which were eusocial, perennial, and cavity-nesting, occupied diverse substrates, including tree trunks, building walls, rock crevices, electric poles, and field ridges—tree trunks and walls being the most common. Wild nests were located at heights ranging from 0 to 13.46 m, mostly around 2 m. Nest entrances varied in shape (circular, oval, slit-like, or irregular), with a longest opening axis of 10.50 ± 2.94 mm, and were oriented in multiple directions. Internally, nests measured 198.31 ± 86.36 mm in length and 142.73 ± 17.28 mm in width. Nests featured brood zones surrounded by honey and pollen pots, along with structure-supporting elements like the involucra and pillars. Brood cells were light brown and oval; those for workers and drones were similar, while queen cells were larger. Honey pots were light to dark brown, oval, dome-shaped, or irregular. Each involucrum was a thin, flat sheet, and the pillar was short, narrow, thread-like. These findings offer valuable insights into the distribution, nesting behaviour, and nest architecture of Tetragonula pagdeni, supporting its conservation and sustainable management. Full article

Stingless bee cerumen is a mixture of wax and plant resins. Foragers of stingless bees are exposed to various chemical contaminants during their plant visits and collection activities. These contaminants have the potential to be transferred into the nest. This study aimed to elucidate the existence of chemical contaminants in Ecuadorian cerumen. To this end, the following aims were established: (i) to determine and quantify glyphosate (GLY), aminomethylphosphonic acid (AMPA), some other pesticides, metals and metalloids in cerumen and (ii) to establish possible risks associated with the presence of these chemical contaminants to the health of stingless bees and humans. The quantification of chemical contaminants was conducted using gas chromatography (GC), liquid chromatography (LC), and ion chromatography (IC) coupled to mass spectrometry (MS). Glyphosate (0.02–0.2 mg/kg) and AMPA (0.028 mg/kg) were detected in four of the pooled samples (n = 14) from the northern and southern highland regions. Other pesticide traces were not detected in any cerumen samples. Metals (Cd, Cr, Pb, Ni, Sn) and metalloids (As, Sb, Se) were found in all samples, including highlands and the lower Amazon. The potential risks of exposure to glyphosate and AMPA for stingless bees and humans appear to be minimal (except for the specific conditions given for Tetragonisca angustula) and safe, respectively. It seems that cerumen may serve as an effective biomonitoring matrix for assessing the environmental health of stingless bee nests. Establishing guidelines and regulations for the safe use and handling of products derived from the stingless bee consumption is therefore imperative. Full article

Understanding wild bee diversity is critical for pollinator conservation, particularly in understudied tropical regions like coastal Ecuador. This preliminary study provides a photography-based assessment of bee diversity at Finca Botánica, an organic and regenerative farm on Ecuador’s central Pacific coast. Over a 10-day survey in December 2024, researchers documented 51 bee species across four families, with Apidae being the most represented. The study highlights a predominance of solitary, ground-nesting bees and a lower-than-expected diversity of Meliponini (stingless bees) and Euglossini (orchid bees) compared to other regions of Ecuador. Many species were found in forest patches, ecological corridors, and cover-cropped maize fields, underscoring the role of sustainable farming practices in supporting pollinator diversity. While photographic methods provided valuable preliminary data, they also revealed limitations in species-level identification, reinforcing the need for future specimen-based surveys. These findings suggest that Ecuador’s dry coastal forests may harbor a richer bee community than previously recognized and that organic farms can serve as important refuges for native pollinators. Full article

Propolis is a substance produced by bees from the collection of plant resins, with a chemical composition that varies according to the available flora and region, and it has several biological activities. Stingless bee propolis is often produced in reduced amounts, posing a challenge to the study of their volatile compounds, as traditional hydrodistillation extraction would demand more raw propolis than available. These bees collect resins from various sources, resulting in a variable composition, so a standardized reproducible method is fundamental for their analysis. Headspace solid-phase microextraction (HS-SPME), associated with gas chromatography, appears to be an efficient alternative for the analysis of these volatiles. In this study, the GC-MS results of three types of SPME fibers were compared to those of extracts obtained by hydrodistillation to evaluate their efficiency in representing the composition of essential oils from (geo)propolis of different species. The extraction time and temperature were also standardized. Among the fibers tested, PDMS/DVB extracted the volatiles in a similar manner to the essential oil obtained by hydrodistillation for all the samples tested, indicating this to be the best choice of fiber coating for propolis volatile extraction and analysis. Full article

This paper introduces a new optimization problem-solving method based on how the stingless bee Tetragonula carbonaria builds and regulates temperature in the hive. The Tetragonula carbonaria Optimization Algorithm (TGCOA) models three different behaviors: strengthening the structure’s hive when it is cold, building combs in a spiral pattern at medium temperatures, and stabilizing the hive when it is hot. These temperature-dependent strategies dynamically balance global exploitation and local exploration within the solution space, enabling a more efficient search. To validate the efficiency and effectiveness of the proposed method, the TGCOA algorithm was tested using ten unimodal and ten multimodal benchmark functions, twenty-eight constrained problems with dimensions set to 10, 30, 50, and 100 taken from the IEEE CEC 2017, and seven real-world engineering design challenges. Furthermore, it was compared with ten algorithms from the literature. Wilcoxon signed-rank and Friedman statistical tests were performed to assess the outcomes. The results on the benchmark problems showed that the approach outperformed 80% of the algorithms at a 5% significance level in the Wilcoxon signed-rank test and ranked first overall according to the Friedman test. Additionally, in multidimensional problems, the TGCOA was ranked first in dimensions 30, 50, and 100. Moreover, in engineering problems, the approach demonstrated a high capacity to solve constraint problems, obtaining better results than the algorithms that were compared. Full article

Crop fidelity is a desirable trait for managed pollinators and is influenced by factors like competing forage sources and colony knowledge of the surrounding environment. In European honey bees (Apis mellifera L.), colonies deployed when the crop is flowering display the highest fidelity. We tested for a similar outcome using a stingless bee species that is being increasingly used as a managed pollinator in Australian macadamia orchards. We observed Tetragonula carbonaria (Smith) colonies deployed in macadamia orchards at three time points: (1) before crop flowering (“permanent”), (2) early flowering (“early”), and (3) later in the flowering period (“later”). We captured returning pollen foragers weekly and estimated crop fidelity from the proportion of macadamia pollen they collected, using light microscopy. Pollen foraging activity was also assessed via weekly hive entrance filming. The early and later introduced colonies initially exhibited high fidelity, collecting more macadamia pollen than the permanent colonies. In most cases, the permanent colonies were already collecting diverse pollen species from the local environment and took longer to shift over to macadamia. Pollen diversity increased over time in all colonies, which was associated with an increase in the proportion of pollen foragers. Our results indicate that stingless bees can initially prioritize a mass-flowering crop, even when flowering levels are low, but that they subsequently reduce fidelity over time. Our findings will inform pollinator management strategies to help growers maximize returns from pollinator-dependent crops like macadamia. Full article

Bacterial symbionts play an important role in insect survival by contributing to key metabolic and defensive functions. While stingless bees are known to harbor diverse microbial communities, their core bacterial symbionts remain poorly characterized. In this study, we analyzed the gut microbiota of sixteen stingless bee species collected from different regions of Mexico using 16S rRNA gene sequencing on the Illumina^® MiSeq™ platform. Our results revealed that Proteobacteria, Firmicutes, and Actinobacteria are the most abundant bacterial phyla across species. Among the dominant genera, lactic acid bacteria, such as Lactobacillus spp., Bifidobacterium, and Fructobacillus spp., were the most prevalent. These bacteria are responsible for developing biochemical functions in metabolic processes like lactic fermentation and the biotransformation of complex organic compounds into molecules that are more easily assimilated by bees. This study offers a novel perspective on the diversity and predicted composition of gut microbiota in Mexican stingless bees. By highlighting differences in microbial communities among species with different feeding habits, our results emphasize the importance of preserving microbial biodiversity in these pollinators. Full article

MicroRNAs (miRNAs) are key post-transcriptional regulators involved in a wide range of biological processes in insects, yet little is known about their roles in stingless bees. Here, we present the first characterization of miRNAs in Melipona quadrifasciata using small RNAs (sRNAs) deep sequencing. A total of 193 high-confidence mature miRNAs were identified, including 106 M. quadrifasciata-exclusive sequences. Expression profiling revealed that mqu-miR-1 and mqu-miR-276 together accounted for over 70% of all miRNA reads, suggesting their central roles in development and reproduction. Comparative analyses showed a higher conservation of M. quadrifasciata miRNAs with other Hymenopterans, especially Apis mellifera and Bombus spp. Putative target genes were predicted using a consensus approach, and functional annotation indicated their involvement in diverse biological regulatory pathways. This work represents the first comprehensive identification of the miRNA repertoire in stingless bees using sRNAs and provides a valuable foundation for understanding miRNA-mediated gene regulation in this ecologically and economically important pollinator. Full article

Group A rotavirus (RV) causes gastrointestinal disease in infants worldwide, and there is currently no specific treatment to eliminate the virus. Due to its chemical properties, propolis is a promising compound for improving gastrointestinal infections. This study aimed to evaluate the action of stingless bee propolis against RV. The method involved determining the concentrations of the extracts that do not exhibit cytotoxicity in colon adenocarcinoma cells using the MTT assay and measuring the reduction in infectivity through a focus forming assay. The results showed that stingless bee propolis was non-cytotoxic up to 200 µg/mL. The reduction in RV infectivity exceeded 99% when using propolis from Plebeia droryana and Melipona quadrifasciata. Brazilian stingless bee propolis, whose active components are known for their activity against various viruses, was experimentally tested and demonstrated effective antiviral activity against RV, supporting its potential application as an antiviral agent. Full article