Search Results (3,294)

Background/Objectives: Spontaneous remission (SR) of acute myeloid leukemia (AML) offers unique clinical insights into host anti-tumor immunity. However, the comprehensive clinical landscape and molecular dynamics of blast clearance and subsequent relapse remain unclear. This study aimed to elucidate these dynamics. Methods: We conducted a two-phase observational study: a systematic pooled analysis of 66 adult AML SR cases (1990–2024) to define clinical triggers and outcomes and longitudinal molecular tracking of two institutional cases to map clonal shifts (with immune profiling for Patient 1 and genomic tracking for both). Results: In the pooled analysis, infection was the predominant trigger, accounting for 78.6% (95% CI: 65.6–88.4%) of SR events. The dataset showed male predominance and monocytic leukemia enrichment (57.6% [95% CI: 44.1–70.4%]), suggesting lineage-specific susceptibility. SR duration and relapse risk were independent of the infection trigger, AML subtype, or age. When integrated with these clinical patterns, institutional tracking was consistent with a biphasic evolutionary model: an acute IL-8 surge alongside NKT and CD4+ T cell activation coincided with blast clearance, as observed primarily in Patient 1. Subsequently, the emergence of TP53 or NRAS mutations within persistent DNMT3A-mutated clones during relapse raised the hypothesis that unresolved chronic inflammation could potentially exert selective pressure favoring resistant subclones. Such interpretations remain correlational and require prospective validation. Conclusions: Our findings outline a clinical–evolutionary framework for AML SR. Remission durability likely relies on balancing acute immune activation with underlying clonal stability. These observational insights highlight complex immune-genomic crosstalk, generating hypotheses for future prospective investigations. Full article

Developing practical low-cost adsorbents for dye-contaminated wastewater remains a critical challenge, especially for persistent cationic dyes such as crystal violet (CV). Here, raw rockmelon skin (RMS), an abundant fruit-processing residue, and its NaOH-modified derivative (NaOH-RMS) were investigated as adsorbents for CV adsorption. Alkaline treatment altered the biomass’s characteristics and affected its adsorption behaviour. Equilibrium was reached within 120 min, and the kinetic data were best fit by the pseudo-second-order model. Equilibrium analysis showed that the Freundlich model best described RMS. In contrast, NaOH-RMS was better represented by the Langmuir model, indicating that alkaline treatment altered the adsorption behaviour of the biomass surface. The Langmuir-derived maximum adsorption capacities were 343.7 mg g⁻¹ for RMS and 295.2 mg g⁻¹ for NaOH-RMS, indicating that NaOH modification did not increase the maximum adsorption capacity. Adsorption was spontaneous across 298–343 K, and both materials retained satisfactory removal performance over five regeneration cycles, particularly under basic desorption conditions. Overall, NaOH treatment altered the adsorption behaviour from heterogeneous adsorption on RMS to a more Langmuir-type adsorption pattern on NaOH-RMS, despite not increasing the maximum adsorption capacity. These findings support the valorisation of fruit-processing residues as practical adsorbents for dye-contaminated wastewater. Full article

Celastrol (CSL), a natural triterpenoid extracted from Tripterygium wilfordii, demonstrates a wide range of biological activities. In this study, we explored whether CSL alleviates kidney damage in spontaneously hypertensive rats (SHRs) through the modulation of the Nrf2/Ho-1 pathway, a crucial target in renal injury models. A total of 40 male SHRs, aged 6–8 weeks, were randomly allocated to four groups: the control group (CON, serving as the healthy control), the spontaneously hypertensive rat group (SHR), the SHR group treated with low-dose CSL (L-CSL + SHR, 0.5 mg/kg/d), and the SHR group treated with high-dose CSL (H-CSL + SHR, 1 mg/kg/d). All drugs were formulated using physiological saline as the solvent and administered via intraperitoneal injection. The control group received an equivalent volume of physiological saline via intraperitoneal injection, and all groups underwent continuous daily administration for 6 weeks. The results indicated that, in comparison with the control group, the serum levels of angiotensin, angiotensin-converting enzyme, and aldosterone in the SHR group were relatively high, and CSL treatment further downregulated these indices. Simultaneously, CSL downregulated pro-inflammatory factors (tumor necrosis factor-α and interleukin-1β) and upregulated interleukin-6. Regarding renal function-related indicators, CSL reduced malondialdehyde levels and enhanced the activities of antioxidant enzymes, such as superoxide dismutase, glutathione peroxidase, and catalase. Moreover, CSL inhibited the overexpression of Keap1. Significantly, the mRNA levels of Nrf2, Nqo1, and Ho-1 in the CSL-treated groups were notably higher than those in the SHR group. These findings suggest that CSL mitigates renal pathological damage in SHR by activating the Nrf2/Ho-1 pathway, offering a potential therapeutic approach for hypertension-induced renal injury. Full article

Background: Achieving facial harmony in patients with micrognathia requires precise chin augmentation. While conventional ready-made implants often fail to conform to unique mandibular surfaces, expensive patient-specific options like PEEK or Titanium lack intraoperative adjustability. We introduce an innovative, cost-effective workflow utilizing 3D-printed templates to fabricate customized Polymethyl Methacrylate (PMMA) implants, emphasizing their clinical feasibility and intraoperative versatility. Methods: We retrospectively analyzed 20 patients with mild-to-moderate micrognathia (<6 mm advancement) who underwent genioplasty between March 2021 and June 2022. Patient-specific templates were produced via Fused Deposition Modeling (FDM) using low-shrinkage Acrylonitrile Butadiene Styrene (ABS) filament. During surgery, final PMMA implants were molded using these sterilized templates. Accuracy was evaluated by comparing mental advancement across preoperative, virtual simulation, and 6-month postoperative stages using Vectra 3D scanning. Results: Quantitative analysis revealed high fidelity between virtual planning and clinical outcomes. The mean discrepancy in horizontal advancement was only 1.02 mm (Planned: 5.04 mm vs. Actual: 4.02 mm). Statistical analysis showed a strong positive correlation (r = 0.928, p = 0.001). Subjective patient satisfaction was high, with 90% reporting “exceptional” or “very improved” results on the Global Aesthetic Improvement Scale (GAIS). Two cases of transient numbness resolved spontaneously within two months. Conclusions: This workflow combines FDM-based template fabrication with intraoperative PMMA molding, enabling real-time adjustment of implant geometry. The results demonstrate a high level of agreement between virtual planning and postoperative outcomes, supporting the clinical reliability of this approach. It may serve as a practical alternative to conventional CAD/CAM methods, particularly in cases requiring both precision and intraoperative flexibility. Full article

Sunset Yellow is a water-soluble synthetic dye resistant to degradation and stable under various conditions, posing an environmental challenge. In the present study pure chitosan hydrogel (PCH) films were synthesized, followed by the assessment of sorption capacity and recyclability compared to chitosan-based films doped with niobium oxide (CHN) or activated carbon (CHC). The aim was to promote the application of sorption methods for Sunset Yellow dye using these films as a treatment option for the pollutant, with the analysis of the effectiveness of the method and its behavior using adsorption kinetic models and thermodynamic analysis. Equilibrium was reached at 240 min for all films tested, with the adsorbed amounts ranging from 18.58 to 18.79 mg g⁻¹ at 30 °C, when the highest kinetic rate constants were observed. The pseudo-first-order kinetic model best described the experimental data, with the lowest Bayesian information criterion, Akaike information criterion, and mean absolute error values. Thermodynamic analysis indicated a spontaneous, exothermic process, with interactions ranging from electrostatic interactions in CHC and PCH to physisorption in CHN. Recycling tests showed 80% efficiency after the third cycle for all three films. These findings highlight the potential of chitosan-based films as an efficient option for removing Sunset Yellow dye from water, thus improving water quality and enhancing wastewater treatment. Full article

Chaotic itinerancy—irregular switching among metastable collective states—provides a dynamical substrate for flexible social coordination, yet its mechanistic origin in multi-agent systems remains unclear. We present a multi-agent Active Inference model in which chaotic itinerancy emerges from Expected Free Energy minimisation without outcome-level social priors. Agents select actions to minimise Expected Free Energy while updating preferences through a precision-gated learning mechanism modulated by interpersonal trust. Hill-function nonlinearity in state transitions creates bistable “affordance landscapes” that gate behavioural mode switching. Simulations with small number of agents on an Erdos–Rényi trust network reveal spontaneous alternation among multiple metastable behavioural clusters, heavy-tailed dwell-time distributions, and sign-changing finite-time Lyapunov exponents—three hallmarks of chaotic itinerancy. Crucially, replacing Hill-function dynamics with linear transitions reduces the chaotic-itinerancy detection rate from 80% to 20%, demonstrating that nonlinear affordance structure is necessary for generating metastable switching. We further show that agents with simplified internal models of the world sustain richer itinerant dynamics as a group than “perfect-foresight” agents, suggesting that bounded rationality may be functionally advantageous for maintaining behavioural flexibility. These results establish active inference as a principled framework for modelling chaotic itinerancy in social systems and offer a computational account of trust-mediated collective transitions observed in theatre workshops and group dynamics. Full article

Metanil Yellow (MY), a highly toxic azo dye used in food products, was removed from aqueous solution using a metal- and halide-free ordered mesoporous carbon (OMC) adsorbent. MY exhibited a strong affinity towards OMC in batch as well as column operations, and OMC performed much better than previously reported adsorbents. The pH, dye concentration, adsorbent dosage, and contact time were optimised, and detailed adsorption experiments were performed under these conditions. Several isotherm models were fitted to the adsorption data, showing that the Langmuir and the Freundlich adsorption models were followed. Adsorption was spontaneous and endothermic at all measurement temperatures. On the basis of pH studies, enthalpy data, and adsorption isotherm analysis, adsorption was determined to be by physisorption. In kinetics studies, the adsorption process was found to be pseudo-second order with interparticle diffusion as the rate-limiting step. Column experiments using a fixed bed of OMC resulted in almost 100% column efficiency and a fractional column capacity of 0.999. During adsorption/desorption cycles of the exhausted column, 99.71% of the dye was recovered after the first cycle and 97.66% after the eleventh. These findings indicate that OMC is a promising and efficient material for the adsorptive removal of toxic MY dye. Full article

We present a short review dedicated to low-lying meson states. We present all meson nonets, which consist from up, down and strange light quarks. We consider the scalar nonet as a basic nonet. We work in the framework of the massless Nambu–Jona-Lasinio $U_R\left(3\right)\times U_L\left(3\right)$ quark model. The collective meson states are described through initially bare quark–antiquark pairs, whose condensates lead simultaneously to spontaneous breaking of the chiral and the flavour symmetry. After quantisation and the spontaneous breaking of the chiral symmetry, when quarks obtain constituent nonzero masses, they become dressed. We present an explanation of the inverse mass hierarchy of the low-lying nonet of the scalar mesons. The proposed explanation is based on symmetry principles. It is shown that, due to the flavour symmetry breaking, two isodoublets of $K_0^{*}\left(700\right)$ mesons play the role of Goldstone bosons. It is also proven that there exists a solution with almost degenerate masses of the $a_0\left(980\right)$ and $f_0\left(980\right)$ mesons and a zero mass of the $f_0\left(500\right)$ meson. Short description of the physical properties of other meson nonets is provided. In particular unique mass relations among the different nonets, which are experimentally confirmed, are presented. Full article

Background: Cataract is a progressive lens opacity. According to the World Health Organization, about 45 million people in the world are blind, with about half of these cases attributable to cataracts. Due to the complexity of cataract disease, current research on cataracts is far from sufficient, so it is especially important to understand the development process and the pathogenic factors of cataracts. Myodes rufocanus (M. rufocanus) is an animal of the M. rufocanus of the hamster family Volinae. In developing M. rufocanus, we found an individual of M. rufocanus with a congenital cataract phenotype. We confirmed the symptoms of cataract under natural light and using a slit lamp. Methods: Therefore, we analyzed the mechanism of congenital cataract in M. rufocanus from the aspects of pathological histology, physiology and biochemistry, and gene level, aiming to explore the feasibility of its development into an animal model of cataract. Cataract is a progressive lens opacity and a leading cause of visual impairment. Understanding its pathogenesis requires appropriate animal models. In a laboratory-bred colony of M. rufocanus, we identified individuals with a spontaneous congenital cataract phenotype, confirmed by gross observation and slit lamp examination. To characterize this phenotype and explore its potential as an animal model, we performed pathological, physiological, biochemical, and transcriptomic analyses using three cataract-affected and three normal age-matched male individuals (8 weeks old per group). Results: Blood tests revealed significantly lower white blood cell and lymphocyte counts in the cataract group, while blood glucose and other biochemical parameters showed no significant differences. Histologically, cataractous lenses exhibited eosinophilic aggregation in the nuclear region with disorganized fiber cells. Transcriptome analysis identified 6544 differentially expressed genes, including downregulation of crystallin genes (CRYBB2, CRYBA4, CRYGS) known to be associated with congenital cataract. KEGG pathway enrichment analysis highlighted retinol metabolism, tyrosine metabolism, and cytochrome P450-related pathways. RT-qPCR confirmed reduced CRYBB2 expression in cataractous eyes. Conclusions: This study provides the first transcriptome dataset for M. rufocanus ocular tissues and offers preliminary evidence that this naturally occurring cataract phenotype may serve as a potential model for congenital cataract research. Full article

Urban stormwater runoff often contains toxic metals that threaten aquatic environments. Meanwhile, the large quantities of drinking water treatment residuals (DWTRs) generated worldwide offer opportunities for sustainable reuse as pollutant removal materials. In this study, a manganese sand-modified drinking water treatment residual particle (RDP-M) was prepared from DWTRs and manganese sand for Pb(II) removal from water. Characterization by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) showed that RDP-M had a rough surface morphology and abundant oxygen-containing functional groups, which provided adsorption sites. Batch experiments showed that the maximum Pb(II) adsorption capacity of RDP-M reached 2.79 mg g⁻¹ at 298 K and pH 7.0, which was about 48% higher than that of the unmodified particles (RDP). The adsorption process followed the pseudo-second-order kinetic model and the Langmuir isotherm model, indicating a chemisorption-dominated process. Thermodynamic analysis further showed that the process was spontaneous and exothermic. RDP-M maintained stable Pb(II) removal over a wide pH range, showed low sensitivity to coexisting ions, and retained high efficiency during repeated use. These results demonstrate that RDP-M has potential as a sustainable granular material for stormwater treatment and waste resource valorization. Full article

Background/Objective: This study evaluated the analgesic and anti-inflammatory effects of ambroxol in an experimental model of endometriosis. Methods: Ambroxol was administered at doses of 10, 50, and 100 mg/kg (Abx 10, Abx 50, and Abx 100) by daily gavage for 21 days. A medroxyprogesterone-treated group (Progesterone) was included as a positive control. Pain was assessed using validated behavioral tests, including the Rat Grimace Scale (RGS), the von Frey test, and the rotarod test. Additionally, interleukin-1β (IL-1β) levels and total leukocyte counts were measured in peritoneal lavage fluid. The volumetric reduction in endometriotic implants was evaluated by ultrasonography, while histopathological analysis characterized inflammatory infiltrate and epithelial layer integrity using a standardized scoring system. Results: All ambroxol doses reduced spontaneous pain manifestations throughout the treatment. The mechanical withdrawal threshold significantly increased from the second week onward, and motor quality improved over the course of the study. A significant reduction in IL-1β levels compared with the negative control (Control(−)) was observed on day 21. Abx 50 and Abx 100 significantly reduced implant volumes (48.2% and 56.2%, respectively) and promoted marked disruption of the endometriotic epithelial layer. When compared with Progesterone, higher doses—particularly 100 mg/kg—demonstrated comparable efficacy. Conclusions: Taken together, these pleiotropic effects support the potential for drug repurposing in endometriosis. Full article

Critical-sized bone defects represent a major clinical challenge, as defects of this magnitude do not heal spontaneously without regenerative intervention. This study aimed to evaluate the osteoinductive effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded β-tricalcium phosphate (β-TCP) scaffolds on bone regeneration and vascularization in a rabbit calvarial critical-sized defect model. Eighteen male New Zealand White rabbits were used, and four standardized circular defects (5 mm in diameter) were created in the calvaria of each animal. The defects were assigned to four groups: control (unfilled), β-TCP + 5 µg rhBMP-2, β-TCP + 10 µg rhBMP-2, and β-TCP + 20 µg rhBMP-2. Bone healing was evaluated at 2, 4, and 8 weeks using histological, histomorphometric, and cluster of differentiation 31 (CD31) immunohistochemical analyses. The results demonstrated that rhBMP-2–loaded β-TCP scaffolds significantly enhanced bone regeneration compared with the control group, with a progressive increase in bone formation observed with increasing rhBMP-2 doses. The β-TCP + 20 µg rhBMP-2 group exhibited the highest levels of new bone formation, more advanced bone maturation, improved collagen organization, and increased vascularization. However, no statistically significant differences were observed between the 10 µg and 20 µg groups at later time points (p > 0.05), suggesting a dose-dependent saturation (plateau) effect. In conclusion, rhBMP-2–loaded β-TCP scaffolds promote bone regeneration and angiogenesis in a dose-related manner up to a threshold, beyond which additional increases in dose do not result in proportional improvements. These findings emphasize that optimal rhBMP-2 dosing is critical to maximize regenerative outcomes while avoiding unnecessary dose escalation. Full article

Semi-coking wastewater generated during coal pyrolysis contains extremely high concentrations of refractory organic pollutants, resulting in elevated chemical oxygen demand (COD) and posing significant environmental risks, making efficient COD removal a critical challenge for sustainable wastewater treatment in the coal chemical industry. In this study, a porous carbon-based resin (XDA-1G) was investigated as an adsorbent for COD removal from semi-coking wastewater. The adsorption performance and underlying mechanisms were systematically evaluated through adsorption isotherm, kinetic, and thermodynamic analyses, combined with structural characterization using FTIR, XPS, BET, XRD, and SEM–EDS. The resin exhibited a high COD removal efficiency of up to 91% with a maximum adsorption capacity of 2182 mg g⁻¹. Kinetic analysis followed the pseudo-second-order model, while the Freundlich isotherm best described the equilibrium behavior, indicating heterogeneous adsorption. Thermodynamic parameters confirmed that the adsorption process is spontaneous and endothermic. Spectroscopic and structural analyses revealed that COD removal is mainly governed by synergistic mechanisms including π–π interactions between aromatic pollutants and the carbon framework, hydrogen bonding with oxygen-containing functional groups, and pore filling within the hierarchical porous structure. These findings demonstrate the strong potential of porous carbon-based resins as efficient adsorbents for treating high-strength industrial wastewater. Full article

Background/Objectives: Patient-derived mesenchymal stem cells (MSCs) represent a promising avenue for myocardial regeneration, yet therapeutic application remains limited by inconsistent differentiation capacity and the absence of standardized cardiogenic induction protocols. This study demonstrates a proof-of-concept for guiding patient-specific bone marrow MSCs toward a functional cardiomyocyte phenotype using paracrine signals from differentiating iPSC-derived cardiomyocytes (iPSC-CMs). Materials and Methods: MSCs were maintained in conditioned medium from a concurrent, validated iPSC-CM differentiation protocol, with evaluation via immunocytochemistry, optical mapping, and whole-cell patch-clamp recordings. Results: Differentiated MSCs acquired organized sarcomeric architecture with cross-striations and displayed spontaneous calcium oscillations with decay kinetics matching source iPSC-CMs (CaT50 ≈ 283 ms vs. 301 ms). In co-culture, MSC-derived cells exhibited synchronized calcium dynamics with iPSC-CMs, confirming functional coupling, while patch-clamp detected hallmark cardiac ion currents (INa, ICa,L, and IKv). Morphologically, MSC-CMs displayed more mature, elongated rod-like shapes. Conclusions: Although current densities indicate partial immaturity, their reproducible detection validates successful cardiomyogenic commitment. This “parallel differentiation” platform eliminates donor-specific protocol tuning, providing a streamlined, paracrine-mediated approach to generate autologous cardiomyocyte-like cells for disease modeling, pharmacological testing, and future regenerative applications. Full article

The burgeoning scale of Pre-trained Large Models (PLMs) has intensified the demand for efficient inference without compromising performance, while existing large model collaborative frameworks have shown promise, they often suffer from functional redundancy among experts and limited robustness in complex cross-domain scenarios. In this paper, we propose Tri-gate Routing for Inference via Decoupled Efficient Network Technologies (TRIDENT), a highly efficient and robust heterogeneous collaborative inference framework. TRIDENT leverages the complementary inductive biases of MLP (for statistical patterns) and KAN (for symbolic logic) to maximize reasoning potential with minimal parametric overhead. To address feature homogenization in traditional distillation, we introduce Orthogonal Feature Decoupling Distillation, utilizing an orthogonality loss ${\mathcal{L}}_{orth}$ for functional decoupling and a reconstruction loss ${\mathcal{L}}_{recon}$ to anchor decoupled features to the PLM knowledge manifold. During inference, a Dual-Threshold Arbiter effectively detects expert hallucinations by integrating individual confidence ${\tau }_{con}$ and heterogeneous consistency ${\tau }_{agree}$. Extensive experiments on CIFAR-100-LT, XNLI, and GSM8K demonstrate that TRIDENT significantly reduces the Invocation Rate (IR) of PLMs while maintaining high accuracy. Our findings reveal a distinct Pareto optimal balance and validate the spontaneous division of labor between heterogeneous experts. By transcending the limitations of single-architecture systems, TRIDENT provides a robust and interpretable pathway for efficient collaborative intelligence. Full article