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15 pages, 2913 KB  
Article
Enhancing Treated Wastewater Reuse in Saudi Agriculture: Farmers’ Perspectives
by Rady Tawfik, Khalid G. Biro Turk, Mohammad Alomair, Salah Sidahmed, Randah M. Alqurashi, Ammar Ebrahim, Mohamed El-Kafrawy, Sidiq Hamad and Emad Al-Karablieh
Sustainability 2025, 17(17), 7633; https://doi.org/10.3390/su17177633 - 24 Aug 2025
Abstract
The reuse of treated wastewater (TWW) offers a sustainable solution for water management in agriculture, particularly in arid regions like Saudi Arabia. However, its success depends on farmers’ acceptance, influenced by perceptions of economic benefits, social acceptability, environmental impacts, and health risks. This [...] Read more.
The reuse of treated wastewater (TWW) offers a sustainable solution for water management in agriculture, particularly in arid regions like Saudi Arabia. However, its success depends on farmers’ acceptance, influenced by perceptions of economic benefits, social acceptability, environmental impacts, and health risks. This study surveys 391 farmers across five regions in Saudi Arabia to assess their attitudes toward TWW reuse in irrigation, exploring how advanced wastewater treatment technologies can improve acceptance. Results show that 65% of farmers use TWW, with usage peaking at 72% in Al-Ahsa and Qatif, driven by water scarcity and lack of alternatives. While 78% are satisfied with TWW, concerns persist regarding pests, consumer acceptance, health risks, and soil quality. Advanced technologies can mitigate these issues by enhancing water quality and safety. The highest positive impact of the use of TWW in irrigation from was the impact on productivity, reduction in the cost of fertilizers and savings in the cost of water abstraction. With only 57% of farmers receiving extension services, integrating education on these technologies could further boost confidence. This study highlights key acceptance factors, underscoring the need for technological and educational interventions to promote sustainable TWW reuse in agriculture. Full article
(This article belongs to the Special Issue Advances in Technologies for Wastewater Treatment and Reuse)
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18 pages, 330 KB  
Article
Bacterial Isolates from Avocado Orchards with Different Agronomic Management Systems with Potential for Promoting Plant Growth in Tomate and Phytopathogen Control
by Adilene Velázquez-Medina, Evangelina Esmeralda Quiñones-Aguilar, Ernestina Gutiérrez-Vázquez, Nuria Gómez-Dorantes, Gabriel Rincón-Enríquez and Luis López-Pérez
Microorganisms 2025, 13(9), 1974; https://doi.org/10.3390/microorganisms13091974 - 23 Aug 2025
Viewed by 64
Abstract
The bacterial diversity of soils cultivated with avocado (Persea americana M.) is influenced by different factors, perhaps the most decisive being the type of agronomic management used by farmers. In conventional agronomic management (CM), high doses of agrochemicals are applied, in contrast [...] Read more.
The bacterial diversity of soils cultivated with avocado (Persea americana M.) is influenced by different factors, perhaps the most decisive being the type of agronomic management used by farmers. In conventional agronomic management (CM), high doses of agrochemicals are applied, in contrast to organic agronomic management (OM), where organic fertilizers are used. This alters the diversity and abundance of soil microorganism populations, which in turn affects crop health. This study aimed to isolate and morphologically characterize rhizospheric bacteria from avocado trees under different agronomic management systems (CM and OM). For the bacterial isolates, their ability to promote plant growth in vitro was determined through biochemical tests for phosphorus and calcium solubilization and nitrogen fixation. In addition, their in vivo effect on tomato (S. lycopersicum) growth was evaluated, and their antagonistic capacity against Fusarium sp. was assessed. The results showed differences in the quantity, diversity, and morphologies of bacterial isolates depending on the type of agronomic management. A higher Shannon diversity index was found in OM (2.44) compared to CM (1.75). A total of 35 bacterial isolates were obtained from both management types. A greater number of isolates from OM soils exhibited in vitro PGP activity; notably, eight isolates from OM plots showed phosphate-solubilizing activity, compared to only one from CM plots. Furthermore, although all isolates demonstrated nitrogen fixing capacity, those from OM orchards produced significantly higher nitrate levels than the control (Azospirillum vinelandii). On the other hand, inoculation of tomato plants with bacterial isolates from OM soils increased plant height, root length, and total fresh and dry biomass compared to isolates from CM soils. Likewise, OM isolates exhibited greater antagonistic activity against Fusarium sp. These findings demonstrate the impact of agronomic management on soil bacterial populations and its effect on plant growth and protection against pathogens. Full article
(This article belongs to the Special Issue Advances in Plant–Soil–Microbe Interactions)
13 pages, 603 KB  
Article
Evaluation of Impacts and Sustainability Indicators of Construction in Prefabricated Concrete Houses in Ecuador
by Marcel Paredes and Javier Perez
Sustainability 2025, 17(17), 7616; https://doi.org/10.3390/su17177616 - 23 Aug 2025
Viewed by 54
Abstract
The construction of prefabricated concrete houses in Ecuador poses significant challenges in terms of environmental and social sustainability, amid growing housing demand and the urgent need to mitigate adverse impacts associated with the construction processes and materials. In particular, the lack of a [...] Read more.
The construction of prefabricated concrete houses in Ecuador poses significant challenges in terms of environmental and social sustainability, amid growing housing demand and the urgent need to mitigate adverse impacts associated with the construction processes and materials. In particular, the lack of a comprehensive assessment of these impacts limits the development of effective strategies to improve the sustainability of the sector. In addition, in rural areas, the design of flexible and adapted solutions is required, as evidenced by recent studies in the Andean area. This study conducts a comprehensive assessment of the impacts and sustainability indicators for prefabricated concrete houses, employing international certification systems such as LEED, BREEAM, and VERDE, to validate various relevant environmental and social indicators. The methodology used is the Hierarchical Analytical Process (AHP), which facilitates the prioritization of impacts through paired comparisons, establishing priorities for decision-making. Hydrological, soil, faunal, floral, and socioeconomic aspects are evaluated in a regional context. The results reveal that the most critical environmental impacts in Ecuador are climate change (28.77%), water depletion (13.73%) and loss of human health (19.17%), generation of non-hazardous waste 8.40%, changes in biodiversity 5%, extraction of mineral resources 12.07%, financial risks 5.33%, loss of aquatic life 4.67%, and loss of fertility 3%, as derived from hierarchical and standardization matrices. Despite being grounded in a literature review and being constrained due to the scarcity of previous projects in the country, this research provides a useful framework for the environmental evaluation and planning of prefabricated housing. To conclude, this study enhances existing methodologies of environmental assessment techniques and practices in the construction of precast concrete and promotes the development of sustainable and socially responsible housing in Ecuador. Full article
(This article belongs to the Special Issue Sustainable Approaches for Developing Concrete and Mortar)
16 pages, 447 KB  
Article
Evaluation of Insect Farming Residue (Frass) as a Phosphate Fertilizer Within the Context of the Circular Economy
by Juan Nieto-Cantero, Miguel A. Romero-Gil, Gina P. Suárez-Cáceres, Antonio Delgado and Víctor M. Fernández-Cabanás
Agronomy 2025, 15(9), 2019; https://doi.org/10.3390/agronomy15092019 - 22 Aug 2025
Viewed by 162
Abstract
Phosphorus (P) stock scarcity is driving the need to develop alternatives to mineral fertilizers. The growing production of insects for high-protein feed results in significant amounts of residues (frass), which can be used as fertilizers. However, its efficiency as such a basic indicator [...] Read more.
Phosphorus (P) stock scarcity is driving the need to develop alternatives to mineral fertilizers. The growing production of insects for high-protein feed results in significant amounts of residues (frass), which can be used as fertilizers. However, its efficiency as such a basic indicator for promoting the recycling of these residues has been rarely assessed. This work aimed to evaluate the efficiency of frass as a P fertilizer. To this end a study was conducted involving P fractionation of frass from two different species (TM: Tenebrio molitor and BSF: black soldier fly or Hermetia illucens) together with vermicompost and a 48-day pot experiment with lettuce (Lactuca sativa). In both frasses, water-soluble P and organic P accounted for more than 30% and 50% of total P, respectively. These P fractions explained the short- and long-term effects of frasses as P fertilizer, which showed a higher P use efficiency than mineral phosphate and vermicompost, with mineral fertilizer replacement values (MFRVs) of 150 and 180% for BSF and TM frass, respectively. Additionally, frass increased P bioavailability in soils more than superphosphate and boosted C and P cycling, thereby enhancing the soil P availability to plants. Therefore, frasses can be effective alternatives to mineral P fertilizers which also contribute to the enhancement of soil health indicators. Full article
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23 pages, 3667 KB  
Article
Multispectral Remote Sensing Monitoring Methods for Soil Fertility Assessment and Spatiotemporal Variation Characteristics in Arid and Semi-Arid Mining Areas
by Quanzhi Li, Zhenqi Hu, Yanwen Guo and Yulong Geng
Land 2025, 14(8), 1694; https://doi.org/10.3390/land14081694 - 21 Aug 2025
Viewed by 153
Abstract
Soil fertility is the essential attribute of soil quality. Large-scale coal mining has led to the continuous deterioration of the fragile ecosystems in arid and semi-arid mining areas. As one of the key indicators for land ecological restoration in these coal mining regions, [...] Read more.
Soil fertility is the essential attribute of soil quality. Large-scale coal mining has led to the continuous deterioration of the fragile ecosystems in arid and semi-arid mining areas. As one of the key indicators for land ecological restoration in these coal mining regions, rapidly and accurately monitoring topsoil fertility and its spatial variation information holds significant importance for ecological restoration evaluation. This study takes Wuhai City in the Inner Mongolia Autonomous Region of China as a case study. It establishes and evaluates various soil indicator inversion models using multi-temporal Landsat8 OLI multispectral imagery and measured soil sample nutrient content data. The research constructs a comprehensive evaluation method for surface soil fertility based on multispectral remote sensing monitoring and achieves spatiotemporal variation analysis of soil fertility characteristics. The results show that: (1) The 6SV (Second Simulation of the Satellite Signal in the Solar Spectrum Vector version)-SVM (Support Vector Machine) prediction model for surface soil indicators based on Landsat8 OLI imagery achieved prediction accuracy with R2 values above 0.85 for all six soil nutrient contents in the study area, thereby establishing for the first time a rapid assessment method for comprehensive topsoil fertility using multispectral remote sensing monitoring. (2) Long-term spatiotemporal evaluation of soil indicators was achieved: From 2015 to 2025, the spatial distribution of soil indicators showed certain variability, with soil organic matter, total phosphorus, available phosphorus, and available potassium contents demonstrating varying degrees of increase within different ranges, though the increases were generally modest. (3) Long-term spatiotemporal evaluation of comprehensive soil fertility was accomplished: Over the 10 years, Grade IV remained the dominant soil fertility level in the study area, accounting for about 32% of the total area. While the overall soil fertility level showed an increasing trend, the differences in soil fertility levels decreased, indicating a trend toward homogenization. Full article
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17 pages, 2930 KB  
Article
Legacy Effects of Different Preceding Crops on Grain Yield, Protein Fractions and Soil Nutrients in Subsequent Winter Wheat
by Rui Wang, Jiayun Wu, Yang Wang, Zhimei Sun, Wenqi Ma, Cheng Xue and Huasen Xu
Plants 2025, 14(16), 2598; https://doi.org/10.3390/plants14162598 - 21 Aug 2025
Viewed by 226
Abstract
Given the pressing global food security crisis and climate change-induced constraints on agricultural productivity, crop rotation proves critical for boosting yield and grain quality of winter wheat (Triticum aestivum) alongside ameliorating soil quality. However, the legacy effect of different preceding crops [...] Read more.
Given the pressing global food security crisis and climate change-induced constraints on agricultural productivity, crop rotation proves critical for boosting yield and grain quality of winter wheat (Triticum aestivum) alongside ameliorating soil quality. However, the legacy effect of different preceding crops on synergistic increments of wheat productivity and soil fertility remains to be fully clarified. Five different preceding crop–winter wheat rotations were conducted in a field experiment established in Huanghua, China. Maize (Zea mays), sorghum (Sorghum bicolor), and millet (Setaria italica) were designated as preceding gramineous crops, and soybean (Glycine max) and mung bean (Vigna radiata) were assigned as preceding legume crops. Grain yield, protein fraction, and soil nutrients were measured to elucidate the legacy effect of the preceding crops on the subsequent winter wheat. Leguminous predecessors significantly evaluated the grain yield of winter wheat compared to gramineous predecessors, particularly that the mung–winter wheat rotation (Mun-W) was 11.56% higher than that of the maize–winter wheat rotation (Mai-W). This rising yield was attributed to the increase of 4.05% in spike number per hectare and 14.31% in kernel number per spike. The Mun-W facilitated the highest gluten protein content (8.22%) in winter wheat among five treatments, which was 6.06% higher than that in the sorghum–winter wheat system. Soil organic matter (SOM) showed an advantage in legume–winter wheat rotations (Leg-Ws) compared to gramineous crop–winter wheat systems (Gra-Ws). Notably among these, the Mun-W significantly enhanced SOM content by 0.99% relative to the Mai-W. The soybean–winter wheat system decreased soil pH by 0.36 compared to the Mai-W system. Coupling coordination degree (CCD) and co-benefit index (CBI) in the Leg-Ws exhibited significant superiority of 62.41% and 42.22% over the Gra-Ws, respectively, and the Mun-W attained maximum CCD by 0.84 and CBI by 0.77. From a multi-objective assessment perspective of the legacy effect of the preceding crops, legume-based rotations facilitate synergistic improvements of yield, protein quality, and soil nutrients in winter wheat. Full article
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22 pages, 6426 KB  
Article
Predicting Soil Fertility in Semi-Arid Agroecosystems Using Interpretable Machine Learning Models: A Sustainable Approach for Data-Sparse Regions
by Nurullah Acir
Sustainability 2025, 17(16), 7547; https://doi.org/10.3390/su17167547 - 21 Aug 2025
Viewed by 281
Abstract
The accurate assessment of soil fertility is critical for guiding nutrient management and promoting sustainable agriculture in semi-arid agroecosystems. In this study, a machine learning-based Soil Fertility Index (SFI) model was developed using regularized regression techniques to evaluate fertility across a dryland maize-growing [...] Read more.
The accurate assessment of soil fertility is critical for guiding nutrient management and promoting sustainable agriculture in semi-arid agroecosystems. In this study, a machine learning-based Soil Fertility Index (SFI) model was developed using regularized regression techniques to evaluate fertility across a dryland maize-growing region in southeastern Türkiye. A total of 64 composite soil samples were collected from the Batman Plain, characterized by alkaline and salinity-prone conditions. Five soil chemical indicators, electrical conductivity (EC), pH, organic matter (OM), zinc (Zn), and iron (Fe), were selected for SFI estimation using a standardized rating approach. The dataset was randomly split into training (80%) and test (20%) subsets to calibrate and validate the models. Ridge, Lasso, and Elastic Net regression models were employed to predict SFI and assess variable importance. Among these, the Lasso model achieved the highest predictive accuracy on test data (R2 = 0.746, RMSE = 0.060), retaining only EC and Zn as significant predictors. Ridge and Elastic Net captured OM and pH, though their contributions were minimal (|β| < 0.01). Spatial predictions showed moderate alignment with observed SFI values (range: 0.48–0.76), but all models underestimated high-fertility zones (>0.69), likely due to coefficient shrinkage. Despite its simplicity, the Lasso model offered superior interpretability and spatial resolution. The results reveal the potential of interpretable machine learning for supporting sustainable, site-specific fertility assessment and informed nutrient management in data-scarce and environmentally vulnerable regions. Full article
(This article belongs to the Section Soil Conservation and Sustainability)
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24 pages, 7566 KB  
Article
Deconstruction of the Crop Rotation Pattern for Saline-Alkaline Land Based on Geo-Information Tupu and Assessment of Its Regulatory Effects on Soil Fertility
by Hui Zhang, Wenhui Cheng and Guoming Du
Sustainability 2025, 17(16), 7430; https://doi.org/10.3390/su17167430 - 17 Aug 2025
Viewed by 287
Abstract
As an important reserve resource for cultivated land, the improvement and fertility enhancement of saline-alkali land are key to alleviating the pressure on cultivated land and ensuring the sustainable utilization of land resources. Studying the regulatory effect of rotation patterns on the soil [...] Read more.
As an important reserve resource for cultivated land, the improvement and fertility enhancement of saline-alkali land are key to alleviating the pressure on cultivated land and ensuring the sustainable utilization of land resources. Studying the regulatory effect of rotation patterns on the soil fertility of saline-alkali land is one of the core research contents in exploring low-cost and environmentally friendly comprehensive management strategies for saline-alkali land. This study focuses on Zhaoyuan County, a representative saline and alkaline area within the Songnen Plain. Utilizing remote sensing technology, crop information was systematically collected across 13 time periods spanning from 2008 to 2020. These data were employed to construct a comprehensive crop information change atlas. This atlas categorized crop rotation patterns based on crop combinations, rotation frequencies, and the number of consecutive years of planting. Using soil sampling data from 2008 and 2020, a soil fertility evaluation was conducted, and the changes in soil chemical properties and fertility under various crop rotation patterns were analyzed. The results of the study show that, during the study period, crop rotation patterns in Zhaoyuan County were dominated by paddy-upland rotations and upland crop rotations. Crop rotation patterns, categorized by crop combination, were dominated by soybean–maize–other crops rotation (S-M-O) and rice–soybean–maize–other crops rotation (R-S-M-O). The frequency of crop rotation is dominated by low- and medium-frequency crop rotation. Crop rotation significantly increased soil organic matter, total nitrogen content, and overall soil fertility in the study area, while simultaneously lowering soil pH levels. Crop rotation patterns with different crop combinations had significant effects on soil chemical properties, with smaller differences in the effects of different rotation frequencies and years of continuous cropping. Crop rotation patterns incorporating soybean demonstrate a significant positive regulatory impact on the soil fertility of saline-alkali land. Low-frequency crop rotation (with ≤5 crop changes) has a relatively better effect on improving soil fertility. This research provides important empirical support and decision-making references for establishing sustainable farming systems in ecologically fragile saline-alkali areas, ensuring regional food security, and promoting the long-term sustainable utilization of land resources. Full article
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19 pages, 1124 KB  
Article
Assessing the Potential Agronomic Value of Spent Mushroom Substrates: Evaluating Their Suitability to Contribute to Soil Carbon Storage
by María R. Yagüe, José A. González-Pérez, Gonzalo Almendros and M. Carmen Lobo
Sustainability 2025, 17(16), 7335; https://doi.org/10.3390/su17167335 - 14 Aug 2025
Viewed by 392
Abstract
The EU’s Circular Economy Action Plan promotes the use of organic waste as fertilizer, thus allowing the recycling of nutrients in the agricultural system. Research on the agronomic reuse of composted substrates previously employed for mushroom cultivation remains limited, despite their rich content [...] Read more.
The EU’s Circular Economy Action Plan promotes the use of organic waste as fertilizer, thus allowing the recycling of nutrients in the agricultural system. Research on the agronomic reuse of composted substrates previously employed for mushroom cultivation remains limited, despite their rich content of plant residues and fungal biomass, which could be repurposed as soil amendments under suitable conditions. This study evaluated the agronomic potential of spent mushroom substrates from Agaricus bisporus and Pleurotus ostreatus, including recomposted A. bisporus residues. A range of analytical procedures was employed to assess their suitability for soil improvement and the formation of humic-like substances, including physical, chemical, microbiological, phytotoxicity, and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) analyses. The spent Pleurotus substrate exhibited low nutrient content (1.1% N, negligible P, 0.9% K), but high water retention (820 kg water Mg−1) and 48% organic carbon (OC), indicating its potential as a soil amendment or seedling substrate. In contrast, spent and composted Agaricus substrates showed moderate nutrient content (1.8–2.7% N; 0.8–0.7% P and 1.3–1.8% K), appropriate C/N ratios (10–15), and sufficient OC levels (24–30%), supporting their use as fertilizers. However, elevated salinity levels (18–23 dS m−1) may restrict their application for salt-sensitive crops. No significant phytotoxic effects on seed germination were observed, and microbiological analyses confirmed the absence of Salmonella spp. in the three substrates. Py-GC/MS revealed a humic acid-like fraction comprising altered lignin structures enriched with lipid and nitrogen compounds. Overall, the studied materials demonstrate promising agronomic value and the capacity to contribute to long-term soil carbon storage. Full article
(This article belongs to the Section Resources and Sustainable Utilization)
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19 pages, 4735 KB  
Article
Evaluation of the Effect of Tenebrio molitor Frass on the Growth Parameters of Canasta Lettuce (Lactuca sativa var. capitata) as a Model Plant
by Simona Errico, Paola Sangiorgio, Salvatore Dimatteo, Stefania Moliterni, Raffaella Rebuzzi, Gerardo Coppola, Catia Giovanna Lopresto and Alessandra Verardi
Agriculture 2025, 15(16), 1731; https://doi.org/10.3390/agriculture15161731 - 12 Aug 2025
Viewed by 334
Abstract
The European Commission approval of some insect species for human consumption, starting with Tenebrio molitor (TM) in 2021, has drawn attention to the production of insect-derived protein flours and the sustainability of insect-rearing systems, particularly on a large scale. This has also highlighted [...] Read more.
The European Commission approval of some insect species for human consumption, starting with Tenebrio molitor (TM) in 2021, has drawn attention to the production of insect-derived protein flours and the sustainability of insect-rearing systems, particularly on a large scale. This has also highlighted the importance of utilizing byproducts, such as frass, and obtaining high-value-added products, such as biofertilizers. This study explored the potential for TM frass (TMF) to serve as a natural fertilizer for the cultivation of Canasta lettuce (Lactuca sativa var. capitata). Specifically, a series of tests was carried out to assess the efficacy of thermal treatment and to verify the trend of certain chemical and growth parameters as a function of the TMF percentage to be added to the potting soil. For this purpose, different percentages of both thermal-treated and untreated TMF and their effects on various growth parameters of Canasta lettuce were evaluated through pot trials. Furthermore, TMF was characterized by using scanning electron microscopy (SEM) to gain insights into its structural features and potential influence on soil–plant interactions. Our results show that heat treatment of TMF is essential to ensure plant survival, and at least in pots, TMF percentages above 5% of soil dry weight are not recommended. In our tests, the most suitable percentage was 4%. Full article
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17 pages, 1264 KB  
Article
Agronomic Potential of Compost from Unconventional Organic Waste Sources and the Effect of Trichoderma harzianum T-22 on Durum Wheat’s Early Development
by Pilar Mañas and Jorge De las Heras
Agronomy 2025, 15(8), 1935; https://doi.org/10.3390/agronomy15081935 - 11 Aug 2025
Viewed by 411
Abstract
Composting organic waste enhances soil fertility, supports plant growth, and offers a sustainable waste management strategy. This study evaluated the agronomic potential of three compost types derived from unconventional sources: (i) sewage sludge, (ii) slaughterhouse animal by-products (ABPs), and (iii) cheese industry waste. [...] Read more.
Composting organic waste enhances soil fertility, supports plant growth, and offers a sustainable waste management strategy. This study evaluated the agronomic potential of three compost types derived from unconventional sources: (i) sewage sludge, (ii) slaughterhouse animal by-products (ABPs), and (iii) cheese industry waste. The impact of Trichoderma harzianum strain T-22 inoculation was also assessed in relation to the early development of durum wheat (Triticum turgidum subsp. durum) under greenhouse conditions. Compost type significantly influenced plant emergence and growth, with sewage sludge-based compost showing the best performance. T. harzianum T-22 inoculation produced mixed results; it improved growth in specific combinations (CS-1/3 and CA-1/4) but inhibited it in others (particularly CW-based composts). These findings underscore the importance of compost source selection and highlight that microbial inoculation effects are substrate-dependent. This work supports sustainable composting practices and fungal waste valorization to optimize plant growth in sustainable agriculture. Full article
(This article belongs to the Section Agricultural Biosystem and Biological Engineering)
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26 pages, 970 KB  
Review
A Review on the Degradation of Antibiotic Resistance Genes During Composting of Livestock Manure
by Enwang Zhao, Yongchao Li, Jin Zhang and Bing Geng
Toxics 2025, 13(8), 667; https://doi.org/10.3390/toxics13080667 - 8 Aug 2025
Viewed by 416
Abstract
As emerging pollutants, antibiotic resistance genes (ARGs) have been recognized as originating from diverse sources. Among these, the use of livestock feed and veterinary drugs was identified as the primary source of ARGs in livestock manure. ARGs were found to be widely distributed [...] Read more.
As emerging pollutants, antibiotic resistance genes (ARGs) have been recognized as originating from diverse sources. Among these, the use of livestock feed and veterinary drugs was identified as the primary source of ARGs in livestock manure. ARGs were found to be widely distributed in global environments, particularly in agriculture-related soils, water bodies, and the atmosphere, posing potential threats to ecological environments and human health. This paper reviewed the degradation mechanisms of ARGs during aerobic composting of livestock manure and the safety evaluation of compost products. Aerobic composting was demonstrated to be an effective method for degrading ARGs, primarily through mechanisms such as high-temperature elimination of ARG-carrying microorganisms, reduction in host bacterial abundance, and inhibition of horizontal gene transfer. Factors including the physicochemical properties of the composting substrate, the use of additives, and the presence of antibiotic and heavy metal residues were shown to influence the degradation efficiency of ARGs, with compost temperature being the core factor. The safety of organic fertilizers encompassed multiple aspects, including heavy metal content, seed germination index, and risk assessments based on ARG residues. The analysis indicated that deficiencies existed in areas such as the persistence of thermotolerant bacteria carrying ARGs, the dissemination of extracellular antibiotic resistance genes (eARGs), and virus-mediated gene transfer. Future research should focus on (1) the removal of thermotolerant bacteria harboring ARGs; (2) the decomposition of eARGs or the blocking of their transmission pathways; (3) the optimization of ultra-high temperature composting parameters; and (4) the analysis of interactions between viruses and resistant hosts. This study reviews the mechanisms, influencing factors, and safety assessment of aerobic composting for degrading ARGs in livestock manure. It not only deepens the understanding of this important environmental biotechnology process but also provides a crucial knowledge base and practical guidance for effectively controlling ARG pollution, ensuring agricultural environmental safety, and protecting public health. Additionally, it clearly outlines the key paths for future technological optimization, thus holding significant implications for the environment, agriculture, and public health. Full article
(This article belongs to the Special Issue Antibiotics and Resistance Genes in Environment)
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12 pages, 732 KB  
Article
Ecotoxicological Assessment of Soils Reclaimed with Waste
by Marta Bik-Małodzińska, Kamila Rybczyńska-Tkaczyk and Anna Jakubczyk
Appl. Sci. 2025, 15(16), 8770; https://doi.org/10.3390/app15168770 - 8 Aug 2025
Viewed by 142
Abstract
This study aimed to conduct an ecotoxicological assessment of soils reclaimed with waste, assessing the treatments’ impacts on both plants and the soils themselves. The reclamation experiment was conducted on the former sulfur mine “Jeziórko”. A microplot experiment was established on a slightly [...] Read more.
This study aimed to conduct an ecotoxicological assessment of soils reclaimed with waste, assessing the treatments’ impacts on both plants and the soils themselves. The reclamation experiment was conducted on the former sulfur mine “Jeziórko”. A microplot experiment was established on a slightly clayey sand to assess the possibilities of different technologies for applying mineral wool to degraded soil. The highest toxicity level was observed in the unreclaimed degraded soil. The M index value was 200%, indicating the death of half of the test organisms. At the same time, root growth inhibition reached 75%, indicating significantly limited root system development. The addition of lime and mineral fertilizers contributed to a slight reduction in toxicity—M = 250%, GI = 50%. Application of sewage sludge at a dose of 100 Mg·ha−1 significantly reduced environmental toxicity—M decreased to 333.3% and 500%, and GI to 35% and 10%, respectively. The addition of mineral wool resulted in further improvement. The best results were achieved in the variant where the soil was enriched with lime, sewage sludge and a large volume of mineral wool (400 m3·ha−1). The GI and M levels indicate that, in this variant, soil toxicity was practically eliminated. Full article
(This article belongs to the Special Issue Degraded Soil Treatment and Influence on Biodiversity)
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20 pages, 2425 KB  
Article
Impact of Tillage System and Mineral Fertilization on Weed Suppression and Yield of Winter Wheat
by Felicia Chețan, Adrian Ioan Pop, Cornel Chețan, Ioan Gaga, Alina Șimon, Camelia Urdă, Alin Popa, Roxana Elena Călugăr, Teodor Rusu and Paula Ioana Moraru
Agronomy 2025, 15(8), 1904; https://doi.org/10.3390/agronomy15081904 - 7 Aug 2025
Viewed by 285
Abstract
This study, which began in the 2013/2014 agricultural year, aimed to assess the suitability of two soil tillage systems for wheat cultivation: conventional soil tillage (CS), which involved moldboard plowing to a depth of 28 cm followed by a single pass with a [...] Read more.
This study, which began in the 2013/2014 agricultural year, aimed to assess the suitability of two soil tillage systems for wheat cultivation: conventional soil tillage (CS), which involved moldboard plowing to a depth of 28 cm followed by a single pass with a rotary harrow to prepare the seedbed, and no-tillage (NT). It also sought to analyze the impacts of these systems on weed infestation levels and, consequently, on yield. A moderate level of fertilization was applied. The experimental field was established with a three-year crop rotation system: soybean–winter wheat–maize. The total number of weed species was 30 in CS, the representative species being Xanthium strumarium, and in NT there were 29 species, with Xanthium strumarium, Cirsium arvense, Bromus tectorum, and Agropyron repens predominating. There was an increase in the number of perennials (dicots and monocots). The total dry matter of weeds was 35.4 t ha−1 in CS and 38.8 t ha−1 in NT. After 11 agricultural years, it was found that there were no significant differences between the two soil tillage systems in terms of wheat yield (6.55 t ha−1 in CS and 6.46 t ha−1 in NT). The uneven rainfall negatively affected wheat growth and favored the spread of weeds, especially dicotyledonous ones. Full article
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23 pages, 2081 KB  
Article
Rapid Soil Tests for Assessing Soil Health
by Jan Adriaan Reijneveld and Oene Oenema
Appl. Sci. 2025, 15(15), 8669; https://doi.org/10.3390/app15158669 - 5 Aug 2025
Viewed by 526
Abstract
Soil testing has long been used to optimize fertilization and crop production. More recently, soil health testing has emerged to reflect the growing interest in soil multifunctionality and ecosystem services. Soil health encompasses physical, chemical, and biological properties that support ecosystem functions and [...] Read more.
Soil testing has long been used to optimize fertilization and crop production. More recently, soil health testing has emerged to reflect the growing interest in soil multifunctionality and ecosystem services. Soil health encompasses physical, chemical, and biological properties that support ecosystem functions and sustainable agriculture. Despite its relevance to several United Nations Sustainable Development Goals (SDGs 1, 2, 3, 6, 12, 13, and 15), comprehensive soil health testing is not widely practiced due to complexity and cost. The aim of the study presented here was to contribute to the further development, implementation, and testing of an integrated procedure for soil health assessment in practice. We developed and tested a rapid, standardized soil health assessment tool that combines near-infrared spectroscopy (NIRS) and multi-nutrient 0.01 M CaCl2 extraction with Inductive Coupled Plasma Mass Spectroscopy analysis. The tool evaluates a wide range of soil characteristics with high accuracy (R2 ≥ 0.88 for most parameters) and has been evaluated across more than 15 countries, including those in Europe, China, New Zealand, and Vietnam. The results are compiled into a soil health indicator report with tailored management advice and a five-level ABCDE score. In a Dutch test set, 6% of soils scored A (optimal), while 2% scored E (degraded). This scalable tool supports land users, agrifood industries, and policymakers in advancing sustainable soil management and evidence-based environmental policy. Full article
(This article belongs to the Special Issue Soil Analysis in Different Ecosystems)
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