Search Results (23)

A comprehensive paleoecological study of a forested bog located in the middle taiga subzone of northeastern European Russia was carried out. According to the ¹⁴C radiocarbon dating and botanical composition analysis, the bog began forming 8200 calibrated years ago, evolving in three stages from grassy wetlands to its current state as a pine-Sphagnum peatland. Analysis revealed substantial carbon storage (81.4 kg m⁻²) within the peat deposit. Macrocharcoal particles were consistently present throughout the peat deposits, demonstrating continuous fire activity across the bog’s developing. High charcoal particle accumulation rates occurred not only during warm periods like the Holocene thermal maximum but also during colder and wetter periods. These periods include recent centuries, when high charcoal accumulation rates are likely due to increased human activity. Statistical analysis showed significant relationships between macrocharcoal content and several peat characteristics: higher charcoal levels correlated with increased soil carbon (r = 0.6), greater aromatic compounds (r = 0.8), and elevated polycyclic aromatic hydrocarbons (r = 0.7), all with p < 0.05. These findings highlight how fire has consistently shaped this ecosystem’s development and carbon storage capacity over millennia, with apparent intensification during recent centuries potentially linked to anthropogenic influences on fire regimes in the boreal zone. Full article

Hydrological processes in river systems are changing due to climate variability and human activities, making it crucial to understand and quantify these changes for effective water resource management. This study examines long-term trends in hydroclimate variables (1990–2022) and land use/land cover (LULC) changes (1988, 2002, and 2022) within the Continental Reach of the Gambia River Basin (CGRB). Trend analyses of the Standardized Precipitation-Evapotranspiration Index (SPEI) at 12-month and 24-month scales, along with river discharge at the Simenti station, reveal a shift from dry conditions to wetter phases post-2008, marked by significant increases in rainfall and discharge variability. LULC analysis revealed significant transformations in the basin. LULC analysis highlights significant transformations within the basin. Forest and savanna areas decreased by 20.57 and 4.48%, respectively, between 1988 and 2002, largely due to human activities such as agricultural expansion and deforestation for charcoal production. Post-2002, forest cover recovered from 32.36 to 36.27%, coinciding with the wetter conditions after 2008, suggesting that climatic shifts promoted vegetation regrowth. Spatial analysis further highlights an increase in bowe and steppe areas, especially in the north, indicating land degradation linked to human land use practices. Bowe areas, marked by impermeable laterite outcrops, and steppe areas with sparse herbaceous cover result from overgrazing and soil degradation, exacerbated by the region’s drier phases. A notable decrease in burned areas from 2.03 to 0.23% suggests improvements in fire management practices, reducing fire frequency, which is also supported by wetter conditions post-2008. Agricultural land and bare soils expanded by 14%, from 2.77 to 3.07%, primarily in the northern and central regions, likely driven by both population pressures and climatic shifts. Correlations between precipitation and land cover changes indicate that wetter conditions facilitated forest regrowth, while drier conditions exacerbated land degradation, with human activities such as deforestation and agricultural expansion potentially amplifying the impact of climatic shifts. These results demonstrate that while climatic shifts played a role in driving vegetation recovery, human activities were key in shaping land use patterns, impacting both precipitation and stream discharge, particularly due to agricultural practices and land degradation. Full article

Costa Rica produces a considerable, important quantity of wood residues. This waste can be pyrolyzed to produce charcoals as main products that can be effectively used as an energy source or to immobilize carbon for soil treatment. However, there is a lack of information about the pyrogenic carbonaceous materials (PCMs), such as charcoal or biochar, obtained at different pyrolysis temperatures. Hence, this study aimed to evaluate the quality of PCMs (physical, mechanical, ultimate analysis, and FTIR analysis) and charcoal characteristics (energetic properties and thermogravimetric analysis—TGA) and biochar characteristics (conductivity, pH, initial contact angle, and wetting rates) for four tropical wood residues produced in five temperatures (300 °C, 350 °C, 400 °C, 450 °C, and 500 °C). In general, pyrolysis temperature between 450 °C and 500 °C produced charcoals with lower values of density, moisture content, compression strength, volatiles, H and O content, and higher values of C and ash contents, conductivity, pH, initial contact angle, and wetting rates. FTIR and TGA analyses show that celluloses and lignin are pyrolyzed at these temperatures, so these temperatures are recommended. The range of 300–350 °C is not recommended, as these parameters were inverse. Multivariate analysis shows that (i) PCMs obtained at lower temperatures (300–350 °C) from Dipteryx panamensis, Hieronyma alchorneoides, and Tectona grandis belong to a cluster with poorer properties, indicating that these temperatures are not adequate for pyrolysis of these species; (ii) all the PCMs obtained from Gmelina arborea were grouped into one cluster, suggesting different PCM quality; and (iii) the PCMs produced from D. panamensis, H. alchorneoides, and T. grandis at 400–500 °C were grouped into another cluster with better properties, suggesting this pyrolysis temperature range as the best for these species. Full article

Plant growth-promoting bacteria (PGPRs) have the potential to act as biofertilizers and biopesticides. This study was planned to explore indigenously isolated PGPRs as a potential candidate to control charcoal rot that affects various crops including soybean. Among the four different tested species of PGPRs, Bradyrhizobium japonicum (FCBP-SB-406) showed significant potential to enhance growth and control soil borne pathogens such as Macrophomina phaseolina. Bacillus subtilis (FCBP-SB-324) followed next. Bradyrhizobium japonicum (FCBP-SB-406) reduced disease severity up to 81.25% in comparison to the control. The strain showed a strong fertilizing effect as a highly significant increase in biomass and other agronomic parameters was recorded in plants grown in its presence. The same was supported by the Pearson’s correlation and principal component analysis. A decrease in disease incidence and severity may be due to the induced resistance imparted by the bacterium. This resulted in significant increments in quantities of defense enzymes, including catalase, peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD). A significant production of proteases, catalases and hydrogen cyanide by B. japonicum (FCBP-SB-406) can also be associated to mycoparasitism. The establishment of PGPRs in treated soils also showed positive effects on soil health. Total metabolite profiling of treated plants in comparison to the control showed the upregulation of many flavonoids, isoflavonoids and amino acids. Many of these compounds have been well reported with antimicrobial activities. Bradyrhizobium japonicum (FCBP-SB-406) can be employed for the production of a potential formulation to support sustainable agriculture by reducing the input of synthetic pesticides and fertilizers. Full article

Population growth in the city of Lubumbashi in the southeastern Democratic Republic of the Congo (DR Congo) is leading to increased energy needs, endangering the balance of the miombo woodland in the rural area referred to as the Lubumbashi charcoal production basin (LCPB). In this study, we quantified the deforestation of the miombo woodland in the LCPB via remote sensing and landscape ecology analysis tools. Thus, the analysis of Landsat images from 1990, 1998, 2008, 2015 and 2022 was supported by the random forest classifier. The results showed that the LCPB lost more than half of its miombo woodland cover between 1990 (77.90%) and 2022 (39.92%) and was converted mainly to wooded savannah (21.68%), grassland (37.26%), agriculture (2.03%) and built-up and bare soil (0.19). Consecutively, grassland became the new dominant land cover in 2022 (40%). Therefore, the deforestation rate (−1.51%) is almost six-times higher than the national average (−0.26%). However, persistent miombo woodland is characterised by a reduction, over time, in its largest patch area and the complexity of its shape. Consequently, because of anthropogenic activities, the dynamics of the landscape pattern are mainly characterised by the attrition of the miombo woodland and the creation of wooded savannah, grassland, agriculture and built-up and bare soil. Thus, it is urgent to develop a forest management plan and find alternatives to energy sources and the sedentarisation of agriculture by supporting local producers to reverse these dynamics. Full article

Phytolith analysis is a well-established archaeobotanical tool, having provided important insights into pre-Columbian crop cultivation and domestication across Amazonia through the Holocene. Yet, its use as a palaeoecological tool is in its infancy in Amazonia and its effectiveness for reconstructing pre-Columbian land-use beyond archaeological sites (i.e., ‘off-site’) has so far received little critical attention. This paper examines both new and previously published soil phytolith data from SW Amazonia to assess the robustness of this proxy for reconstructing pre-Columbian land-use. We conducted the study via off-site soil pits radiating 7.5 km beyond a geoglyph in Acre state, Brazil, and 50 km beyond a ring-ditch in northern Bolivia, spanning the expected gradients in historical land-use intensity. We found that the spatio-temporal patterns in palm phytolith data across our soil-pit transects support the hypothesis that pre-Columbian peoples enriched their forests with palms over several millennia, although phytoliths are limited in their ability to capture small-scale crop cultivation and deforestation. Despite these drawbacks, we conclude that off-site soil phytolith analysis can provide novel insights into pre-Columbian land use, provided it is effectively integrated with other land-use (e.g., charcoal) and archaeological data. Full article

Vegetation and fire archives of the late Holocene are essential for understanding the importance of natural and anthropogenic forcings on past and future vegetation successions as well as climate changes. Here we present a 1640-year record of vegetation and fire history of the Lake Haixihai catchment in northwestern Yunnan, southwest China. Pollen and charcoal analyses as well as XRF (X-ray fluorescence) analysis of lacustrine sediments from Lake Haixihai were employed to reveal its regional vegetation, forest fire, and soil erosion intensity changes over the last 1640 years. The results show their significant changes attributed to both climatic conditions and human activities: The lake catchment witnessed the densest forests (including pine and hemlock forests, and evergreen broadleaved forests) and the weakest soil erosion of the last 1640 years as well as relatively frequent forest fires at 380–880 AD, when vegetation succession, forest fire, and soil erosion were mainly driven by natural forcings, i.e., climatic conditions. A significant and abrupt drop in forest density, minor changes in abundance of forest components, and gradually strengthening soil erosion occurred at the transition from 880 to 1040 AD, when anthropogenic forcings such as the development of agriculture and systematic deforestation gradually became dominant ones driving vegetation succession and soil erosion. After this transition, forest density and soil erosion intensity never returned to the level before 880 AD, implying that the modern landscape in the lake catchment was already established approximately at as early as ca. 1040 AD. No significant changes in forest component and density as well as forest fire and soil erosion occurred until 1940 AD. The most frequent forest fires, the strongest soil erosion, and low forest density after 1940 AD might be attributed partially to climatic conditions and partially to extensive deforestation around 1960 AD. Full article

The management of crop production in a sandy soil “culture” is difficult, mainly due to its low soil-water-holding capacity, organic matter and poor fertilizer efficiency. Options to increase soil water and nutrient retention for these soils include the addition of surface mulch covers, amendment with biochar and the use of layers of a mixture of charcoal and compost material. Our objective was to measure the distribution of water and nutrients for layers of control 1 (CK1), control 2 (CK2) and compost material of different thicknesses (0.02, 0.05 and 0.10 m) buried 0.01 m from the surface in a column (0.2 m radius, 0.5 m height) filled with sand. The experiment was conducted in a greenhouse located at the Agricultural Science Training Base of Ningxia University, China. There were three replicates per treatment and one soil column per replicate. The soil columns were watered with 2 L via a surface drip emitter and 45 days later, soil samples were obtained in 0.01 m increments across the diameter and depth of 0.4 m, with a total of 12 samples per column. In each sample, we measured soil water, pH, electrical conductivity, ammonium and nitrate nitrogen and available P and K. The results showed that the distribution of water content and nutrient contents were centered on the dripper and diffused to its surroundings. Notably, the horizontal diffusion distance was smaller than that of the vertical direction. In the vertical direction, compared with control 1, adding compost changed the spatial distribution of WC and nutrients and had a greater impact on available potassium (AK) than on inorganic nitrogen (IN) and available phosphorus (AP). Compared with control 1, the composting treatment decreased the content of water in the 0–10 cm surface soil, reduced the electrical conductivity (EC) and nitrate nitrogen (NO₃-N), C5 and C10 increased the available potassium. Moreover, composting treatments increased the electrical conductivity, available phosphorus, available potassium and nitrate nitrogen of the 10–30 cm substrate by 61–384%, 10–240%, 11–45% and 133–929%, respectively, when compared with control 1.The nutrients increased as the thickness of the compost interlayer increased. A principal component analysis (PCA) of the C5 and C10 treatments significantly distinguished them from control 1. A linear regression fitting analysis showed that the inorganic nitrogen, available potassium and total nutrients positively correlated with the water content and electrical conductivity of the sand. The 5 cm and 10 cm composting interlayers had a high water content and ability to conserve fertilizer for sand culture, but C10 caused an excessive accumulation of nutrients. Thus, it was concluded that a composting interlayer that was less than 5 cm reduced the base fertilizer input by 24–84%. All these results suggest that applying a composting interlayer of 5 cm could retain more suitable root zone water and fertilizer for the next crop season and provide technological support to reduce fertilizer inputs. Full article

We studied charcoal from several types of natural soil archives, including cultural layers of archaeological sites (hillforts), surrounding forest and arable soils, and sediments in lower parts of the slopes associated with hillforts and moraine hills. The stratigraphy of the charcoals was described, and 41 samples were radiocarbon-dated. Analysis of 2277 charcoals showed the presence of 13 taxa of woody species; Pinus and Picea charcoals dominated. Charcoals older than 300 BC were found only in sediment and in several pits formed after treefalls with uprooting. The greatest diversity of woody species was found in the hillforts’ cultural layers composed of Anthropogenic Dark Earth soils formed between 300 BC and 300 cal. AD (Early Iron Age). All charcoals from ancient arable soils were younger than charcoals from the hillforts. Charcoals indicated that burning for arable farming started in the study region in the 6th century AD. Woody taxa exhibited a decrease in number of species and a decrease in the proportion of nemoral (broadleaved) species from the Early Iron Age to the Middle Ages and to the present. Quercus, Tilia, and Corylus have become relatively rare, although they still occur in the study region. Ulmus and Acer are now rare in the Upper Volga region and were not found in the vicinity of the study sites. Full article

Based on the analysis of the botanical composition of the organic-mineral soil layer and peat, dendrochronological and radiocarbon datings, we performed the reconstruction of the development of six pine wooded sphagnum bogs located in the boreal zone of Russia. Most of the bogs under study followed the endogenesis patterns with the vegetation cover gradually changing, peat layer growing, substrate trophicity declining and shrub-sphagnous vegetation forming under modern conditions. Emerging pyrogenic layers and charcoals in the peat indicate that the study sites were constantly affected by fires, which periodically interrupted the endogenous development of the bogs, especially during the warmest Holocene periods. Full article

Old-growth forests are of high interest for biodiversity conservation, especially in the disturbance-prone Mediterranean landscapes. However, it remains unclear whether the survival of patches of old-growth forest in the degraded Mediterranean landscapes results from local anomalies or from past, larger forests. Therefore, in this study, we assessed (1) the origin, (2) the long-term ecological trajectory, and (3) the mechanism(s) that explain the survival of a Mediterranean old-growth forest, the Sainte-Baume forest. To achieve this, we used soil charcoal analysis. We opened fifteen soil profiles in the forest and five in its surrounding areas for soil description and sampling. The soil descriptions enabled us to highlight in situ soil horizon and colluvial layers. A total of 1656 charcoal pieces from different soil samples were taxonomically identified to characterize the composition of past forests. Selected charcoal pieces (n = 34) were dated to obtain chronological data. Our investigations indicate that the survival of the Mediterranean old-growth forest, in the context of the semi-open/open Mediterranean landscapes, is the result of a combination of biotic and abiotic factors, which reduced the influence of past forest disturbances. Thus, the resistance and resilience of the forest areas are preserved over a long-term ecological trajectory. Therefore, the potential of Mediterranean old-growth forests as baseline reference points for the conservation of biodiversity is related to the identification and maintenance of the local biotic and abiotic factors which allowed the survival of the old-growth forest. Full article

Soil-available P for crop use is limited because of fixation reaction and loss of organic matter through erosion and surface runoff. These factors cause an imbalance between inputs and outputs of P nutrients in acid soils. Several approaches to improve P availability have been proposed, however, little is known about the effectiveness of amending humid mineral acid soils with charcoal and sago bark ash on P dynamics. Thus, pH buffering capacity and leaching studies were conducted to determine: (i) pH buffering capacity upon application of charcoal and sago bark ash and (ii) the influence of charcoal and sago bark ash on P leaching in acid soils. pH buffering capacity was calculated as the negative reciprocal of the slope of the linear regression (pH versus acid addition rate). A leaching study was carried out by spraying distilled water to each container with soil such that leachates through leaching were collected for analysis. The ascending order of the treatments based on their pH buffering capacity and regression coefficient (R²) were soil alone (0.25 mol H⁺ kg⁻¹ sample), soil with charcoal (0.26 mol H⁺ kg⁻¹ sample), soil with sago bark ash (0.28 mol H⁺ kg⁻¹ sample), charcoal alone (0.29 mol H⁺ kg⁻¹ sample), soil with charcoal and sago bark ash (0.29 mol H⁺ kg⁻¹ sample), and sago bark ash alone (0.34 mol H⁺ kg⁻¹ sample). Improvement in the soil pH buffering capacity was partly related to the inherent K, Ca, Mg, and Na contents of charcoal and sago bark ash. In the leaching study, it was noticed that as the rate of sago bark ash decreased, the pH of leachate decreased, suggesting that unlike charcoal the sago bark ash has significant impact on the alkalinity of leachate. Soil exchangeable acidity, Al³⁺, and H⁺ reduced significantly following co-application of charcoal and sago bark ash with ERP. This could be attributed to the neutralizing effects of sago bark ash and the high affinity of charcoal for Al and Fe ions. The amount of P leached from the soil with 100% charcoal was lower because charcoal has the ability to capture and hold P-rich water. The findings of this present study suggest that combined use of charcoal and sago bark ash have the potential to mitigate soil acidity and Al toxicity besides improving soil pH buffering capacity and minimizing P leaching. A field trial to consolidate the findings of this work is recommended. Full article

Soil acidity compromises agricultural output in tropical acid soils. Highly weathered tropical acidic soils are characterized by low pH, organic matter, nutrient availability, but high aluminium and iron concentration. Hence, N availability becomes a limiting factor in such soils. To this end, these leaching and pH buffering capacity studies were conducted to determine the effects of co-application of charcoal and sago bark ash on the N leaching or retention and pH buffering capacity of acid soils. The soil leaching experiment was conducted for 30 days by spraying distilled water to each container with soil such that the leachates were collected for analysis. The rate of urea used was fixed at 100% of the recommended rate. The rates of charcoal and sago bark ash were varied by 25%, 50%, 75%, and 100%, respectively, of the recommended rates. The pH buffering capacity was calculated as the negative reciprocal of the slope of the linear regression. The leaching study revealed that the combined use of charcoal, sago bark ash, and urea does not only reduce leaching of NH₄⁺ and NO₃⁻ but the approach also improves soil pH, total C, and soil exchangeable NH₄⁺. This effect is related to the fact that the sago bark ash deprotonates the functional groups of charcoal because of its neutralizing components such as Ca, Mg, Na, and K ions. As a result, the combined use of charcoal and sago bark ash was able to retain NH₄⁺ in the soil. The carbonates in the sago bark ash and functional groups of charcoal improve pH buffering capacity. Thus, the combined use of charcoal and sago bark ash improved soil exchangeable NH₄⁺, soil pH, and soil total C, but reduced exchangeable acidity and amount of NH₄⁺ leached out from soil. This study will be further evaluated in a pot trial to confirm the results of the present findings. Full article

The gene pool of Mesoamerican common beans (Phaseolus vulgaris L.) includes genotypes in the small-to-medium-size seeded dry beans, as well as some snap beans from hotter environments adapted to the Southeastern United States. However, the warm and humid climate of the Southeastern United States is conducive to diseases such as Common Bacterial Blight (CBB) and Charcoal Rot (CR). The pathogens for these two diseases can survive long periods in infested soil or on seeds and are difficult to control through pesticides. Hence, field-level resistance would be the best management strategy for these diseases. The goals of this study were (1) to evaluate field-level resistance from the various commercial classes and subgroups represented in the Mesoamerican gene pool as sources for breeding beans for the region and (2) to evaluate genome-wide marker × trait associations (GWAS) using genetic markers for the genotypes. A total of 300 genotypes from the Mesoamerican Diversity Panel (MDP) were evaluated for CBB and CR in field experiments for three years. CBB resistance was also tested with a field isolate in controlled greenhouse conditions. The analysis of variance revealed the presence of variability in the MDP for the evaluated traits. We also identified adapted common bean genotypes that could be used directly in Southeastern production or that could be good parents in breeding programs for CBB and CR resistance. The GWAS detected 14 significant Single-Nucleotide Polymorphism (SNP) markers associated with CBB resistance distributed on five chromosomes, namely Pv02, Pv04, Pv08, Pv10, and Pv11, but no loci for resistance to CR. A total of 89 candidate genes were identified in close vicinity (±100 kb) to the significant CBB markers, some of which could be directly or indirectly involved in plant defense to diseases. These results provide a basis to further understand the complex inheritance of CBB resistance in Mesoamerican common beans and show that this biotic stress is unrelated to CR resistance, which was evident during a drought period. Genotypes with good yield potential for the Southeastern U.S. growing conditions were found with resistant to infection by the two diseases, as well as adaptation to the hot and humid conditions punctuated by droughts found in this region. Full article

Plants emit volatile organic compounds (VOCs) that induce metabolomic, transcriptomic, and behavioral reactions in receiver organisms, including insect pollinators and herbivores. VOCs’ composition and concentration may influence plant-insect or plant-plant interactions and affect soil microbes that may interfere in plant-plant communication. Many Trichoderma fungi act as biocontrol agents of phytopathogens and plant growth promoters. Moreover, they can stimulate plant defense mechanisms against insect pests. This study evaluated VOCs’ emission by olive trees (Olea europaea L.) when selected Trichoderma fungi or metabolites were used as soil treatments. Trichoderma harzianum strains M10, T22, and TH1, T. asperellum strain KV906, T. virens strain GV41, and their secondary metabolites harzianic acid (HA), and 6-pentyl-α-pyrone (6PP) were applied to olive trees. Charcoal cartridges were employed to adsorb olive VOCs, and gas chromatography mass spectrometry (GC-MS) analysis allowed their identification and quantification. A total of 45 volatile compounds were detected, and among these, twenty-five represented environmental pollutants and nineteen compounds were related to olive plant emission. Trichoderma strains and metabolites differentially enhanced VOCs production, affecting three biosynthetic pathways: methylerythritol 1-phosphate (MEP), lipid-signaling, and shikimate pathways. Multivariate analysis models showed a characteristic fingerprint of each plant-fungus/metabolite relationship, reflecting a different emission of VOCs by the treated plants. Specifically, strain M10 and the metabolites 6PP and HA enhanced the monoterpene syntheses by controlling the MEP pathway. Strains GV41, KV906, and the metabolite HA stimulated the hydrocarbon aldehyde formation (nonanal) by regulating the lipid-signaling pathway. Finally, Trichoderma strains GV41, M10, T22, TH1, and the metabolites HA and 6PP improve aromatic syntheses at different steps of the shikimate pathway. Full article