Search Results (19)

Feline tritrichomonosis caused by Tritrichomonas foetus is an important cause of chronic or intermittent large-bowel diarrhea in cats. Nested PCR is useful for detecting low parasite burdens in fecal samples, but gel-based endpoint interpretation requires post-amplification handling and may increase the risk of amplicon contamination. This study describes a one-tube nested real-time PCR-HRM assay for qualitative detection of T. foetus DNA in feline fecal extracts. The assay was adapted from a previously described single-tube nested PCR workflow by integrating real-time fluorescence monitoring and high-resolution melting analysis as a closed-tube endpoint. Analytical evaluation included post-extraction matrix-matched sensitivity testing, an exclusivity panel, matrix blank assessment, a checkerboard carryover experiment, and within-platform repeatability assessment of HRM temperatures. Method agreement was assessed using 147 fecal samples from diarrheic cats and compared with gel-based single-tube nested PCR and outer-primer PCR. Both nested methods detected 16/147 samples, whereas outer-primer PCR detected 5/147 samples. All 16 nested-positive amplicons were confirmed by Sanger sequencing and showed 99.51–100.00% identity to T. foetus reference sequences. Across 95 target-associated dual-peak HRM reactions, the low-temperature melting domain showed a mean Tm of 76.18 ± 0.24 °C, and the high-temperature melting domain showed a mean Tm of 81.57 ± 0.42 °C. However, because no independent reference standard was available, diagnostic sensitivity and specificity were not determined. The assay should therefore be interpreted as a preliminary analytical evaluation and method-agreement study of a closed-tube alternative to gel-based nested PCR. Full article

Real-time monitoring of marine ecosystems is crucial for global climate change research. In extreme marine environments such as the westerly regions in the Arctic and Antarctic, monitoring buoys and platforms often suffer from severe challenges, including insufficient energy supply, limited battery life, and difficult maintenance. Triboelectric nanogenerators (TENGs) offer a promising strategy for self-powered marine sensing. However, conventional tubular liquid–solid triboelectric nanogenerators (LS-TENGs) suffer from low efficiency of interfacial charge transfer due to limited contact area and excessive internal resistance, which restricts their output. In this study, a multi-tube nested liquid–solid triboelectric nanogenerator (MLS-TENG) is proposed, and the suitable filling ratio is determined through comparative experiments on structural parameters. This design significantly increases the effective contact area, reduces internal resistance, and improves synergistic charge transfer at multiple interfaces. Experimental results demonstrate that the MLS-TENG exhibits substantially improved electrical output compared with the corresponding single-tube structures. When integrated with a power management module, the capacitor charging efficiency is improved by approximately 120 times. In real sea trials, an array composed of MLS-TENG units successfully drives a self-powered sensing system, achieving stable 4G transmission of environmental parameters. This work provides a scalable structural optimization strategy for constructing high-performance blue energy-harvesting self-powered nodes for the marine Internet of Things. Full article

Hollow-core anti-resonant fibers (HC-ARFs) have emerged as a promising platform for next-generation optical systems, offering attractive advantages in low-latency, low-nonlinearity, and high-power handling. However, the development of high-performance functional components, such as polarization beam splitters (PBSs), within this platform faces a significant challenge: the simultaneous achievement of ultra-broad bandwidth, compact device length, high polarization selectivity, and strict single-mode operation remains elusive. To address this challenge, we propose and numerically investigate a novel dual hollow-core anti-resonant fiber (DHC-ARF) based on a hybrid nodal–nodeless architecture. The design integrates three functional units: (1) an asymmetric nested semi-elliptical tube pair that defines the dual cores and serves as the primary wavelength-insensitive coupling channel; (2) nodeless nested circular tubes positioned peripherally to effectively suppress higher-order mode propagation while maintaining low fundamental mode loss; and (3) a selective localized thick-wall region that introduces a polarization-dependent perturbation to the x-polarized supermodes, whose observed behavior is physically consistent with a phase-mismatch effect associated with anti-crossing-like modal interaction near the target wavelength. Through synergistic optimization of these elements, we numerically demonstrate a combination of performance metrics. At the central wavelength of 1.55 µm, the coupling length for the y-polarization (Lcy) is reduced to 6.35 cm, while the coupling length ratio (CLR = $L_{\mathrm{cx}}$/$L_{cy}$) equals 2.001, indicating effective polarization selectivity. Consequently, a device length of 12.7 cm is numerically demonstrated, which is comparable to or shorter than existing ultra-broadband DHC-ARF PBS designs. The proposed PBS is numerically shown to exhibit an ultra-broad bandwidth of 460 nm (spanning 1320 to 1780 nm) with a polarization extinction ratio better than 20 dB, peaking at 53 dB. Furthermore, HOMER (λ) remains above 100 throughout the operating band and exceeds 200 over most of the band, indicating robust single-mode operation. This work not only presents a PBS design with competitive overall performance but also provides a versatile structural paradigm for developing functional components in hollow-core fiber-based integrated optical systems for high-speed communications and precision sensing. It should be noted that this work is based on numerical simulations, and experimental fabrication and validation will be pursued in future work. Full article

A novel anti-resonant hollow-core fiber (AR-HCF) is proposed. The fiber uses cross-nested circular cladding tubes including single-nested and non-nested tubes in the near-infrared spectral region of 1.3–1.7 μm. All the anti-resonant tubes are used to minimize the confinement loss (CL) of the LP₀₁ core mode. The non-nested tubes are also employed to achieve single-mode performance through strong mode coupling between the cladding mode and the LP₁₁ core mode. The impact of the structural parameters on the CL of the modes is analyzed by using the finite element method (FEM). Optimization results indicate that the CLs of the LP₀₁ mode and the LP₁₁ mode are 0.18 dB/km and 5.88 × 10³ dB/km at 1.55 μm. Consequently, the higher-order mode extinction ratio (HOMER) achieves 3.27 × 10⁴. Additionally, the mode field area of the fiber exceeds 4720 μm² and the corresponding mode field diameter of the LP₀₁ mode is more than 77 μm across the spectral region of 1.3–1.7 μm. In the practical applications, the fabrication tolerance is analyzed. The collapse of anti-resonant tubes within 0–2 μm and positional offsets between 0° and 4° both have minimal impact on fiber performance, thereby ensuring the stability of the system. Compared with other reported fibers, the proposed fiber demonstrates superior performance. Full article

Spring viremia of carp (SVC) is a highly contagious disease that affects cyprinids, resulting in systemic hemorrhage, abdominal distension, exophthalmia, and high mortality in juveniles. This can lead to significant losses in the aquaculture industry. The World Organization for Animal Health (WOAH) recommends a two-step semi-nested reverse transcription polymerase chain reaction (RT-PCR) method for diagnosis. However, this method is labor-intensive, requires large reagent volumes, and is prone to carry-over contamination. Here, we evaluated the detection sensitivity of one-step semi-nested RT-PCR (combining RT and primary amplification in a single tube, followed by a second nested PCR step) against conventional two-step semi-nested RT-PCR. SVC virus (SVCV) subgroup Ia was tested using cell culture, RT-quantitative PCR, and one-step RT-PCR. The two-step semi-nested PCR method detected viral RNA up to a 10⁻² dilution, whereas one-step semi-nested RT-PCR detected it up to a 10⁻⁵ dilution, showing a 1000-fold improvement in sensitivity. Moreover, detection rates increased from 84.2% with two-step semi-nested RT-PCR to 91.7% with one-step semi-nested RT-PCR in fish tissue samples. One-step semi-nested RT-PCR reduces processing time, minimizes handling steps, and contamination risk, and enhances analytical sensitivity. This supports its adoption as a practical, high-throughput diagnostic tool for SVCV and consideration for future WOAH guidelines. Full article

We propose a new hollow-core fiber design based on a hybrid structure of nested elliptical and semicircular tubes. We numerically investigate the loss and single-mode performance of this design in the communication band and derive the values of each parameter of the fiber cladding structure that theoretically lead to the best performance of the fiber. The resulting structure has a minimum confinement loss as low as 0.00033 dB/km at 1550 nm and an astonishing extinction ratio of 2,439,607 for the higher-order modes, showing excellent loss and single-mode performance. In addition, the design also exhibits excellent bending insensitivity, with the loss gradually dropping well below 0.01 dB/km when the bending radius exceeds 14 cm. The proposed fiber structure has a very promising application in optical communication systems. Full article

This study innovatively presents a hollow-core anti-resonant fiber integrating double-tube nesting and a single-layer anti-resonant wall. Featuring an exclusive two-layer cladding configuration along with an outer cladding circular ring, it differs significantly from traditional fibers. After careful parameter optimization, at 1.55 μm wavelength, the fiber shows excellent performance. Its confinement loss drops to 0.00088 dB/km, 1–2 orders lower than traditional ones. The proportion between the loss of the lowest higher-order mode and that of the fundamental mode reaches 19,900, indicating excellent single-mode performance. In the case of a bending radius of 11–14.2 cm, the x-polarization loss is below 0.001 dB/km, showing good bending resistance. Through structural comparisons, this paper quantitatively reveals the effects of the anti-resonant wall, cladding tube, and outer cladding ring on fiber performance. From the practical fiber-drawing process, it thoroughly analyzes the impact of the outer connecting tube’s offset angle on fiber performance. This research provides crucial theoretical support for new hollow-core fiber design, manufacture, and application, and is expected to drive technological innovation in this field. Full article

The efficient utilization of geothermal energy depends heavily on high-performance ground heat exchangers. Coaxial pipe is a high-efficiency heat exchanger composed of two nested tubes of different diameters. In this paper, the structure and thermal exchange characteristics of coaxial pipe geothermal exchangers are introduced, which are superior to single-U and double-U geothermal exchangers in respect of installation, heat transporting, and deep geothermal application. Thermal test research of coaxial pipe geothermal exchangers is investigated. Relevant studies in recent years on the factors affecting the thermal performance of coaxial pipe ground heat exchangers, including exchanger configurations, circulating fluids, subsurface conditions, flow patterns, and operational modes, are reviewed. In addition, research on the impact of coaxial pipe ground heat exchangers on the ground, as well as applications for coaxial pipe ground heat exchangers, is summarized. Recommendations are made for potential future research on coaxial pipe ground heat exchangers. It is believed that the results of these studies will help to raise awareness of coaxial pipe ground heat exchangers and to continue to promote their application. Full article

The role of the human papillomavirus (HPV) in the establishment of cervical cancer has driven studies to find more effective methods of viral detection so that early intervention strategies can be performed. However, the methods still have limitations, especially regarding detecting the different genotypes simultaneously. We have developed a high-throughput system using a single-tube nested-multiplex polymerase chain reaction (NMPCR) for the detection of 40 HPV genotypes using capillary electrophoresis. The NMPCR assay was compared to the Hybrid Capture 2 assay (HC2) with 40 women from the Northeast of Brazil (São Luis, MA), a high endemic region, where the HPV positivity was 75% and 37.5%, respectively. These results were validated by performing a molecular epidemiological study on 5223 Brazilian women undergoing gynecological examinations from 2009 to 2017, who presented with an HPV prevalence of 59%. Multiple infections were found in 62.5% and 58% of the patients from the endemic region and from the Brazilian women population, respectively, mostly presenting high-risk genotypes (90.5% and 60%, respectively). Considering cervical intraepithelial neoplasia and adenocarcinomas, the sensitivity and specificity were 97.5% and 100%, respectively. The NMPCR assay was also capable of identifying viral subtypes in cases of multiple infections, even with low viral loads (10⁻⁶ ng/µL of HPV DNA). The NMPCR test is a promising and robust tool for HPV diagnostics and a screening tool for prevention of cervical cancer. Full article

The in-plane loading conditions of carbon fiber/epoxy composite (CFRP) and aluminum nested-tube-reinforced expanded polypropylene (EPP) blocks were empirically examined. This study used crashworthiness metrics to estimate the best design configuration under quasi-static loading rates. The experimental phase began with lateral loading testing of single and nested aluminum and CFRP specimen. In-plane crushing experiments were performed on EPP foam blocks reinforced with nested tubes. Both single and nested aluminum tubes had comparable force–response curves and maintained their load-bearing capacity throughout testing. Despite a load-carrying capacity drop above a particular displacement threshold, the CFRP specimens had superior specific energy absorption (SEA) values due to their lightweight nature. The triple-tube nested specimens with two smaller tubes exhibited the best SEA results (1.72 and 1.88 J/g, respectively, for the aluminum and CFRP nested samples). During concurrent tube deformation, the nested samples showed a synergistic connection that increased energy absorption, especially in the EPP foam blocks with reinforced tubes. The study also examined the effects of building nested specimens with aluminum exterior tubes and CFRP inner tubes, and vice versa. This method showed that CFRP tubes within aluminum outer tubes lowered specimen weight (from 93.1 g to 67.7 g) and energy absorption (from 160.2 J to 153.3 J). However, the weight reduction outweighed the energy absorption, increasing SEA values for certain composite material configurations (from 1.72 J/g to 2.26 J/g). Full article

A highly sensitivity balloon-like fiber interferometer based on ethanol coating is presented in this paper. The Mach–Zehnder interferometer is formed by bending a single-mode fiber to a balloon-like structure and nested in the Teflon tube. Then, an ethanol solution was filled into the tube of the balloon-like fiber interferometer by the capillary effect. Due to the high sensitivity of the refractive index (RI) of ethanol solutions to temperature, when the external temperature varies, the optical path difference changes. The change in temperature can be detected by the shift in the interference spectrum. Limited by the size of the balloon-like structure, three kinds of these structures with different sensitive lengths were prepared to select the best parameters. The sensitive lengths were 10, 15 and 20 mm, respectively, and the RI detection performance of each structure in 10~26% NaCl solutions was investigated experimentally. The results show that when the sensitive length is 20 mm, the RI sensitivity of the sensor is the highest, which is 212.88 nm/RIU. Ultimately, the sensitive length filled with ethanol is 20 mm. The experimental results show that the temperature sensitivity of the structure is 1.145 nm/°C in the range of 28.1 °C~35 °C, which is 10.3 times higher than that of an unfilled balloon-like structure (0.111 nm/°C). The system has the advantages of low cost and easy fabrication, which can potentially be used in high-precision temperature monitoring processes. Full article

Thin-walled structures, when used for high-speed railways, can effectively mitigate the irreversible destruction when a malfunction occurs. Nested thin-walled tubes, as energy-absorbing structures, possess excellent specific energy absorption (SEA) and crushing force efficiency (CFE). This paper conducts multi-objective optimization by focusing on a square corrugation nested structure with a double octagon inner wall, namely SCOD, to ameliorate the crashworthiness of the nested structure. The finite element model of the SCOD is constructed and validated by test data. A set of experimental design points with good spatial distribution are obtained using the optimal Latin hypercube (LHC) method. The polynomial response surface (PRS) method was applied to establish the fitting relationship between design variables and optimization objectives, and validation is accomplished. The DCNSGA-III algorithm is employed for optimization, resulting in a Pareto alternative solution set with good population diversity and convergence. In addition, to observe the optimized performance, a set of optimal solutions considering a single objective value is derived, and a comprehensive optimal solution is obtained by applying the minimum distance selection method (TMDSM). Finally, the proposed optimized system is analyzed and validated. According to the alternative reference solutions, the initial peak force (IPCF) reduces by 53.75% and CFE increases by 8.7%. This paper provides some reference for the optimization design in practical engineering. Full article

We present a novel homebuilt scanning tunneling microscope (STM) with atomic resolution integrated into a cryogen-free superconducting magnet system with a variable temperature insert. The STM head is designed as a nested structure of double piezoelectric tubes (PTs), which are connected coaxially through a sapphire frame whose top has a sample stage. A single shaft made of tantalum, with the STM tip on top, is held firmly by a spring strip inside the internal PT. The external PT drives the shaft to the tip–sample junction based on the SpiderDrive principle, and the internal PT completes the subsequent scanning and imaging work. The STM head is simple, compact, and easy to assemble. The excellent performance of the device was demonstrated by obtaining atomic-resolution images of graphite and low drift rates of 30.2 pm/min and 41.4 pm/min in the X–Y plane and Z direction, respectively, at 300K. In addition, we cooled the sample to 1.6 K and took atomic-resolution images of graphite and NbSe₂. Finally, we performed a magnetic field sweep test from 0 T to 9 T at 70 K, obtaining distinct graphite images with atomic resolution under varying magnetic fields. These experiments show our newly developed STM’s high stability, vibration resistance, and immunity to high magnetic fields. Full article

Mode-division multiplexing (MDM) can achieve ultra-high data capacity in optical fiber communication. Several impressive works on multicore fiber (MCF), multi-mode fiber, and few-mode multicore fiber have made significant achievements in MDM. However, none of the previous works can simultaneously maintain the transmission loss, chromatic dispersion (CD), and differential group delay (DGD) at a relatively low level. A nested multiple hollow-core anti-resonant fiber (NMH-ARF) has significant potential for applications in MDM. This study proposes a novel NMH-ARF with its structural design based on the traditional single-core nested anti-resonant fiber. We increased the number of nodes between capillaries. By changing the position of the nested tubes, several interconnected areas form when a single core is separated. We investigated the mode-coupling theory and transmission characteristics of this fiber. This fiber structure showed a low sensitivity to bending and achieved a super-low DGD and a super-low confinement loss (CL) at a wavelength of 1.55 µm while keeping CD relatively low. Full article

In this work, we propose analytical formulas for the estimation of dispersion properties and effective area of the fundamental mode of hollow-core inhibited coupling fibers with a microstructured cladding composed by a ring of dielectric tubes. The formulas are based on a model which has already been successfully applied to the estimation of confinement loss. The model takes into account the effects of the coupling of the fundamental core mode with the cladding modes in the context of the single-tube approximation. Effective index, group velocity dispersion, and effective area of the fundamental mode are estimated and compared with the results obtained from numerical simulations, by considering ten different fibers. The comparison shows a good accuracy of the proposed formulas, which do not require any tuning of fitting parameters. On the basis of the analysis carried out, a scaling law relating the effective area to the core radius is also given. Finally, the formulas give a good estimation of the same parameters of other Hollow-core inhibited coupling fibers, such as nested, ice-cream, and kagome fibers. Full article