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Application of Molecular Biology in Parasitology

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: 20 August 2026 | Viewed by 1382

Special Issue Editors


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Guest Editor
Department of Medical Biology and Biochemistry, Faculty of Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-092 Bydgoszcz, Poland
Interests: parasitic infections; environmental parasitology; molecular techniques in parasitology; oxidative stress; toxoplasmosis; giardiasis

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Guest Editor
Department of Biology, Parasitology and Pharmaceutical Botany, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland
Interests: acanthamoeba; blastocystis; medical parasitology
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Infectious and Invasive Diseases and Veterinary Administration, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, 85-092 Torun, Poland
Interests: parasitology; veterinary parasitology; veterinary medicine; molecular techniques; molecular diagnostics; parasitic diseases

Special Issue Information

Dear Colleagues,

Parasitic diseases continue to pose a significant threat to human and animal health. In routine diagnostics, traditional parasitological methods are sometimes insufficient for accurate identification. Molecular biology techniques are now playing a crucial role in the rapid and effective detection of various pathogens affecting both humans and animals. These methods also enable environmental and food safety testing, detecting contamination, and ensuring public health. Additionally, molecular approaches allow for precise parasite genotyping and the monitoring of disease transmission.

This Special Issue, entitled “Application of Molecular Biology in Parasitology”, focuses on recent advances in molecular techniques applied to parasitology.

Potential topics include, but are not limited to, the following:

  • Molecular diagnostics of parasitic infections;
  • Genetic diversity and genotyping of parasites;
  • Molecular epidemiology and transmission tracking;
  • Detection of parasites in environmental and food samples;
  • Novel molecular tools for parasite identification and characterization;
  • Host–parasite interactions at the molecular level;
  • Advances in parasite genomics and transcriptomics.

We welcome original research articles and reviews that highlight the latest developments in the field.

Dr. Roland Wesołowski
Dr. Karolina Kot
Dr. Dawid Jańczak
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • molecular diagnostics
  • parasitic infections
  • genotyping
  • molecular epidemiology
  • parasite detection
  • environmental parasitology
  • food safety
  • host–parasite interactions
  • genomics and transcriptomics
  • molecular techniques in parasitology

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Published Papers (1 paper)

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Research

14 pages, 1633 KB  
Article
Livestock, Humans, and the Environment as Reservoirs of Cryptosporidium and Giardia duodenalis: Evidence from a One Health Study in Türkiye
by Eylem Akdur-Öztürk, Yaseen Majid Salman Al-Adilee, William Edwards, Jaya Makkimane, Eleni Gentekaki, Funda Dogruman-Al and Anastasios D. Tsaousis
Appl. Sci. 2026, 16(6), 2899; https://doi.org/10.3390/app16062899 - 17 Mar 2026
Viewed by 750
Abstract
Cryptosporidium spp. and Giardia duodenalis are globally important intestinal protozoa causing diarrheal disease in humans and animals, with significant zoonotic potential. This study aimed to investigate the prevalence, molecular diversity, and potential transmission dynamics of these parasites in humans, livestock, and environmental samples [...] Read more.
Cryptosporidium spp. and Giardia duodenalis are globally important intestinal protozoa causing diarrheal disease in humans and animals, with significant zoonotic potential. This study aimed to investigate the prevalence, molecular diversity, and potential transmission dynamics of these parasites in humans, livestock, and environmental samples from a rural community in Türkiye using a One Health approach, and to assess their associations with gut microbiome composition. Faecal samples were collected from 124 humans, 305 livestock (cattle, sheep, and goats), and 40 environmental samples (water and mud). Parasites were detected using qPCR and nested PCR, with positive samples genotyped by sequencing. Microbiome profiling was performed using 16S rRNA gene amplicon sequencing. Giardia duodenalis was detected in 12.1% of humans, 17.5% of livestock, and 2.5% of environmental samples, with assemblages A, B, and E identified, including the first detection of assemblage E in a human in Türkiye. Cryptosporidium spp. were found in 8.9% of humans, 19.3% of livestock, and 55% of environmental samples, with C. parvum as the dominant zoonotic species. Microbiome analysis revealed no significant differences in overall diversity. This study provides the first One Health assessment of Cryptosporidium spp. and Giardia duodenalis in Türkiye, emphasizing zoonotic transmission risks linked to livestock and the environment. Full article
(This article belongs to the Special Issue Application of Molecular Biology in Parasitology)
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