Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

Article Types

Countries / Regions

Search Results (39)

Search Parameters:
Keywords = single-line laser imaging

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
27 pages, 9529 KB  
Article
Simulation-Based Evaluation of a Single-Line Laser Framework for AUV Wall-Following and Mapping
by Yu-Cheng Chou and Jia-Han Huang
J. Mar. Sci. Eng. 2026, 14(7), 680; https://doi.org/10.3390/jmse14070680 - 5 Apr 2026
Viewed by 514
Abstract
This study presents a simulation-based evaluation of a wall-following and mapping framework for autonomous underwater vehicles (AUVs) equipped with a single-line laser, targeting structured environments such as rectangular tanks and dam interiors. A hardware-in-the-loop (HIL) simulation platform is developed to integrate sensor emulation, [...] Read more.
This study presents a simulation-based evaluation of a wall-following and mapping framework for autonomous underwater vehicles (AUVs) equipped with a single-line laser, targeting structured environments such as rectangular tanks and dam interiors. A hardware-in-the-loop (HIL) simulation platform is developed to integrate sensor emulation, vehicle dynamics, and image-based control while preserving the onboard data formats, update rates, and communication protocols of the AUV system. Using a single camera–laser pair, the framework estimates yaw angle and lateral wall distance from laser image geometry to support real-time wall-following and frontal obstacle avoidance. Wall mapping is performed by transforming laser image features into spatial coordinates and estimating the dimensions of geometric protrusions. The framework is evaluated on simulated walls with protruding features under two navigation conditions: ideal-motion and dynamic-control operation. Simulation results show stable wall-following performance, with lateral distance errors typically below 0.1 m. Under ideal-motion conditions, mapping errors range from 1% to 13%, while under dynamic-control navigation they increase to 10–35% due to attitude fluctuations and control-induced motion. Frontal obstacle avoidance maintains a minimum clearance of 1.04 m. The results demonstrate the feasibility of using a single-line laser and a unified image stream for both real-time wall-following control and post-mission geometric mapping within the defined simulation conditions. While the evaluation is limited to simulation and assumes idealized optical conditions without modeling hydrodynamic disturbances or optical degradation effects, the framework provides a system-level reference for laser-guided inspection strategies in confined underwater environments such as tanks, reservoirs, and dams. Full article
(This article belongs to the Section Ocean Engineering)
Show Figures

Figure 1

22 pages, 588 KB  
Review
Evolution of Percutaneous Nephrolithotomy (PCNL) from Standard to Miniaturized and Ultra-Mini Techniques: A Narrative Review
by Mladen Doykov, Jasmin Gurung, Usman Khalid, Gancho Kostov, Bozhidar Hristov, Petar Uchikov, Krasimir Kraev, Lyubomir Chervenkov and Elizabet Karen Dzhambazova
Medicina 2026, 62(3), 484; https://doi.org/10.3390/medicina62030484 - 4 Mar 2026
Viewed by 1016
Abstract
Background and Objectives: Because of its consistently high stone-free rates (SFRs), percutaneous nephrolithotomy (PCNL) continues to be the first-line treatment for renal stones larger than 20 mm. Standard 24 to 30 Fr access tracts, however, are linked to access-related morbidity, such as bleeding, [...] Read more.
Background and Objectives: Because of its consistently high stone-free rates (SFRs), percutaneous nephrolithotomy (PCNL) continues to be the first-line treatment for renal stones larger than 20 mm. Standard 24 to 30 Fr access tracts, however, are linked to access-related morbidity, such as bleeding, pain, and extended hospital stays. These restrictions have led to progressive tract miniaturization and the development of mini-PCNL, ultra-mini PCNL, and micro-PCN techniques. Materials and Methods: We performed a narrative review of studies published through January 2026 using PubMed and Google Scholar. Search terms included percutaneous nephrolithotomy, mini-PCNL, ultra-mini PCNL, micro-PCNL, and vacuum-assisted PCNL. Original studies, systematic reviews, and meta-analyses reporting clinical outcomes, complications, and advancements were selected, whereas conference abstracts, non-English papers, and articles without accessible full text were excluded. Results: Across randomized trials, miniaturized PCNL generally preserves efficacy when patients are selected appropriately. Across randomized trials and meta-analyses, miniaturized PCNL achieved stone-free rates comparable to standard PCNL (typically ~80–90% for stones ≤20 mm and similar rates in selected stones >2 cm), while demonstrating lower hemoglobin decrease (mean difference approximately −0.6 to −1.0 g/dL), reduced transfusion rates, and shorter hospital stays, at the cost of longer operative time (mean difference ~8–12 min). On the other hand, operative time may increase, and smaller working channels can make visualization and fragment evacuation more demanding as stone burden rises. Raised intrarenal pressure is a recurring safety issue because it may increase infectious risk unless drainage is actively managed. Recent innovations aim to address these limitations, including vacuum-assisted access sheaths, pressure-controlled irrigation, improved laser and lithotripsy platforms, image-fusion guidance, navigation systems, and robotic assistance. Conclusions: PCNL now spans a spectrum of tract sizes rather than a single standard approach. When chosen appropriately and performed with attention to pressure control and fragment evacuation, miniaturized PCNL can reduce morbidity without sacrificing stone clearance. Future advancements in percutaneous stone surgery are more likely to rely on integrated technological solutions that improve accuracy, safety, and repeatability than on additional tract size reduction. Full article
(This article belongs to the Section Urology & Nephrology)
Show Figures

Figure 1

15 pages, 4598 KB  
Article
Improved PPIM—A Method to Further Improve the Measurement Accuracy of the Cross-Sectional Area of the Conveying Material Load
by Ning Jiang, Boxuan Shang, Qinghe Ji, Mengchao Zhang and Yuan Zhang
Appl. Sci. 2026, 16(1), 542; https://doi.org/10.3390/app16010542 - 5 Jan 2026
Viewed by 494
Abstract
Timely adjustment of belt conveyor speed according to the conveyed load is a key approach to achieving energy-efficient operation. Line laser-assisted vision has been widely adopted for load measurement, in which image processing techniques are employed to extract and analyze the outer contour [...] Read more.
Timely adjustment of belt conveyor speed according to the conveyed load is a key approach to achieving energy-efficient operation. Line laser-assisted vision has been widely adopted for load measurement, in which image processing techniques are employed to extract and analyze the outer contour of material piles highlighted by laser stripes. To address issues such as laser stripe thinning and breakpoint handling, the point-by-point interpolation method (PPIM) was previously proposed, enabling column-wise extraction of laser stripe pixels by incorporating the geometric characteristics of material accumulation, thereby improving real-time performance. However, its adaptability remains limited under complex pile geometries and strong reflective interference. In this paper, the pixel traversal strategy is further optimized to achieve efficient and robust extraction of the laser stripe centerline. By performing a single, non-global image traversal, laser stripe thinning, breakpoint identification, interpolation, continuity reconstruction, and cross-sectional area calculation are integrated into a unified processing framework. Experimental results demonstrate that the improved method achieves a 0.3% increase in measurement accuracy compared with the original PPIM, while maintaining excellent real-time performance with a processing speed of up to 94 frames per second (FPS). The proposed approach provides a more reliable load perception basis for intelligent speed regulation of belt conveyors, contributing to energy-efficient and stable operation. Full article
(This article belongs to the Special Issue Precision Measurement Technology)
Show Figures

Figure 1

27 pages, 33395 KB  
Article
Deep Line-Segment Detection-Driven Building Footprints Extraction from Backpack LiDAR Point Clouds for Urban Scene Reconstruction
by Jia Li, Rushi Lv, Qiuping Lan, Xinyi Shou, Hengyu Ruan, Jianjun Cao and Zikuan Li
Remote Sens. 2025, 17(22), 3730; https://doi.org/10.3390/rs17223730 - 17 Nov 2025
Cited by 3 | Viewed by 1772
Abstract
Accurate and reliable extraction of building footprints from LiDAR point clouds is a fundamental task in remote sensing and urban scene reconstruction. Building footprints serve as essential geospatial products that support GIS database updating, land-use monitoring, disaster management, and digital twin development. Traditional [...] Read more.
Accurate and reliable extraction of building footprints from LiDAR point clouds is a fundamental task in remote sensing and urban scene reconstruction. Building footprints serve as essential geospatial products that support GIS database updating, land-use monitoring, disaster management, and digital twin development. Traditional image-based methods enable large-scale mapping but suffer from 2D perspective limitations and radiometric distortions, while airborne or vehicle-borne LiDAR systems often face single-viewpoint constraints that lead to incomplete or fragmented footprints. Recently, backpack mobile laser scanning (MLS) has emerged as a flexible platform for capturing dense urban geometry at the pedestrian level. However, the high noise, point sparsity, and structural complexity of MLS data make reliable footprints delineation particularly challenging. To address these issues, this study proposes a Deep Line-Segment Detection–Driven Building Footprints Extraction Framework that integrates multi-layer accumulated occupancy mapping, deep geometric feature learning, and structure-aware regularization. The accumulated occupancy maps aggregate stable wall features from multiple height slices to enhance contour continuity and suppress random noise. A deep line-segment detector is then employed to extract robust geometric cues from noisy projections, achieving accurate edge localization and reduced false responses. Finally, a structural chain-based completion and redundancy filtering strategy repairs fragmented contours and removes spurious lines, ensuring coherent and topologically consistent footprints reconstruction. Extensive experiments conducted on two campus scenes containing 102 buildings demonstrate that the proposed method achieves superior performance with an average Precision of 95.7%, Recall of 92.2%, F1-score of 93.9%, and IoU of 88.6%, outperforming existing baseline approaches by 4.5–7.8% in F1-score. These results highlight the strong potential of backpack LiDAR point clouds, when combined with deep line-segment detection and structural reasoning, to complement traditional remote sensing imagery and provide a reliable pathway for large-scale urban scene reconstruction and geospatial interpretation. Full article
Show Figures

Figure 1

13 pages, 2763 KB  
Article
Structural Deflection Measurement with a Single Smartphone Using a New Scale Factor Calibration Method
by Long Tian, Yangxiang Yuan, Liping Yu and Xinyue Zhang
Infrastructures 2025, 10(9), 238; https://doi.org/10.3390/infrastructures10090238 - 10 Sep 2025
Viewed by 1191
Abstract
This study proposes a novel structural deflection measurement method using a single smartphone with an innovative scale factor (SF) calibration technique, eliminating reliance on laser rangefinders and industrial cameras. Conventional off-axis digital image correlation (DIC) techniques require laser rangefinders to measure discrete points [...] Read more.
This study proposes a novel structural deflection measurement method using a single smartphone with an innovative scale factor (SF) calibration technique, eliminating reliance on laser rangefinders and industrial cameras. Conventional off-axis digital image correlation (DIC) techniques require laser rangefinders to measure discrete points for SF calculation, suffering from high hardware costs and sunlight-induced ranging failures. The proposed approach replaces physical ranging by deriving SF through geometric relationships of known structural dimensions (e.g., bridge length/width) within the measured plane. A key innovation lies in developing a versatile SF calibration framework adaptable to varying numbers of reference dimensions: a non-optimized calculation integrates smartphone gyroscope-measured 3D angles when only one dimension is available; a local optimization model with angular parameters enhances accuracy for 2–3 known dimensions; and a global optimization model employing spatial constraints achieves precise SF resolution with ≥4 reference dimensions. Indoor experiments demonstrated sub-0.05 m ranging accuracy and deflection errors below 0.30 mm. Field validations on Beijing Subway Line 13′s bridge successfully captured dynamic load-induced deformations, confirming outdoor applicability. This smartphone-based method reduces costs compared to traditional setups while overcoming sunlight interference, establishing a hardware-adaptive solution for vision-based structural health monitoring. Full article
Show Figures

Figure 1

18 pages, 2185 KB  
Review
Research Progress on Aging Detection of Composite Insulators Based on Spectroscopy
by Junfei Nie, Yunpiao Cai, Jinke Chen, Furong Chen, Jiapei Cao, Quan Li and Zhenlin Hu
Photonics 2025, 12(9), 905; https://doi.org/10.3390/photonics12090905 - 10 Sep 2025
Cited by 1 | Viewed by 1815
Abstract
The safety of composite insulators in high-voltage transmission lines is directly related to the stable operation of the power system, which is a fundamental condition for the normal functioning of people’s lives and industrial production. Composite insulators are exposed to outdoor conditions for [...] Read more.
The safety of composite insulators in high-voltage transmission lines is directly related to the stable operation of the power system, which is a fundamental condition for the normal functioning of people’s lives and industrial production. Composite insulators are exposed to outdoor conditions for extended periods of time, and with the increase in service life, they are subjected to aging due to external environmental factors and electrical stresses. This aging leads to a decline in their electrical insulation, mechanical properties, and other performance, which, in severe cases, may result in power system failures. Therefore, accurate assessment and detection of the aging status of composite insulators are particularly important. Traditional detection methods such as visual inspection, hardness testing, and hydrophobicity testing have limitations, including single functionality and susceptibility to environmental interference, which cannot comprehensively and accurately reflect the aging condition of the insulators. In recent years, spectroscopy-based detection technologies have been increasingly applied for the rapid detection of composite insulators due to their advantages, such as high sensitivity, non-contact measurement, and multi-dimensional information extraction. Common spectroscopic detection methods include Ultraviolet Discharge (UV Discharge), Fourier Transform Infrared (FTIR) Spectroscopy, Raman Spectroscopy (RS), Hyperspectral Imaging (HSI), Laser-Induced Breakdown Spectroscopy (LIBS), and Terahertz (THz) Spectroscopy. These methods offer non-contact, remote, and rapid capabilities, enabling detailed analysis of the insulator’s surface microstructure, chemical composition, and aging characteristics. This paper introduces = spectroscopy-based methods for detecting the aging status of composite insulators, analyzing the advantages and limitations of these methods, and discussing the challenges of their industrial application. Furthermore, the paper reviews the research progress and practical applications of spectroscopic techniques in the evaluation of insulator aging status, systematically summarizing important achievements in the field and providing an outlook for future developments. Full article
(This article belongs to the Special Issue Advanced Optical Measurement Spectroscopy and Imaging Technologies)
Show Figures

Figure 1

18 pages, 39111 KB  
Article
Impact of Beam Shape and Frequency on Weld Seam Geometry and Penetration Depth Using a Coherent Beam Combining Laser
by Karthik Ravi Krishna Murthy, Reza Sanei, Abhay Sharma, Simon Olschok and Uwe Reisgen
Appl. Sci. 2025, 15(17), 9432; https://doi.org/10.3390/app15179432 - 28 Aug 2025
Cited by 3 | Viewed by 1583
Abstract
The geometry and quality of a weld seam are critical factors in laser beam welding, influencing mechanical performance and structural integrity. Dynamically modulated laser beams provide a precise means of tailoring energy input in high-power laser welding processes. This study investigates the influence [...] Read more.
The geometry and quality of a weld seam are critical factors in laser beam welding, influencing mechanical performance and structural integrity. Dynamically modulated laser beams provide a precise means of tailoring energy input in high-power laser welding processes. This study investigates the influence of beam shape and modulated frequency on weld seam geometry, penetration depth, and capillary behaviour using a coherent beam combining (CBC) laser system from Civan Lasers. Three beam intensity distributions—single point, line–point–line (LPL), and boomerang—were applied across a modulation frequency range of 1, 10, and 100 kHz during the welding of duplex and austenitic stainless steels. High-speed imaging captured real-time capillary dynamics, and the data were analysed to assess capillary stability, measure capillary diameter, and determine the capillary front angle as a function of frequency and beam shape. Transverse cross-sections of the welds were prepared to evaluate seam geometry and microstructure. The results show that beam shape significantly affects energy distribution and weld profile, while modulation frequency critically influences capillary behaviour and penetration characteristics. These findings highlight the critical role of dynamic beam shaping and frequency modulation in optimizing laser welding processes for material-specific performance, offering a versatile platform for advancing precision manufacturing using CBC technology. Full article
(This article belongs to the Special Issue Advanced Welding Technology and Its Applications)
Show Figures

Figure 1

12 pages, 2763 KB  
Article
Damage Characteristics of Silicon Solar Cells Induced by Nanosecond Pulsed Laser
by Hao Chang, Weijing Zhou, Yingjie Ma, Zhilong Jian, Xiaoyuan Quan and Chenyu Xiao
Photonics 2025, 12(8), 804; https://doi.org/10.3390/photonics12080804 - 11 Aug 2025
Viewed by 2045
Abstract
The damage characteristics of monocrystalline silicon solar cells irradiated by a nanosecond pulsed laser were investigated in a vacuum environment. An 8 ns pulsed laser was used with a 1064 nm wavelength, a 2.0 J maximum pulse energy, and a millimeter-scale ablation spot [...] Read more.
The damage characteristics of monocrystalline silicon solar cells irradiated by a nanosecond pulsed laser were investigated in a vacuum environment. An 8 ns pulsed laser was used with a 1064 nm wavelength, a 2.0 J maximum pulse energy, and a millimeter-scale ablation spot diameter. The cells were irradiated by a laser with varying fluences, irradiation positions, and pulse numbers. The damage mechanism was discussed in combination with the degradation of electrical properties, the morphology of surface damage, and electroluminescence images. A single pulse mainly caused surface heating and deformation, while multi-pulse irradiation led to the formation of melting ablation craters. More severe performance degradation was caused by irradiation at the grid line site due to fracture of the grid line electrodes. Moreover, monocrystalline silicon cells showed excellent damage resistance to fixed-position irradiations at non-gridded line areas. This work reveals, for the first time in vacuum, that grid-line fracture dominates performance degradation—enabling targeted hardening for space solar cells. Full article
Show Figures

Figure 1

33 pages, 8582 KB  
Article
Mobile Tunnel Lining Measurable Image Scanning Assisted by Collimated Lasers
by Xueqin Wu, Jian Ma, Jianfeng Wang, Hongxun Song and Jiyang Xu
Sensors 2025, 25(13), 4177; https://doi.org/10.3390/s25134177 - 4 Jul 2025
Cited by 1 | Viewed by 1366
Abstract
The health of road tunnel linings directly impacts traffic safety and requires regular inspection. Appearance defects on tunnel linings can be measured through images scanned by cameras mounted on a car to avoid disrupting traffic. Existing tunnel lining mobile scanning methods often fail [...] Read more.
The health of road tunnel linings directly impacts traffic safety and requires regular inspection. Appearance defects on tunnel linings can be measured through images scanned by cameras mounted on a car to avoid disrupting traffic. Existing tunnel lining mobile scanning methods often fail in image stitching due to the lack of corresponding feature points in the lining images, or require complex, time-consuming algorithms to eliminate stitching seams caused by the same issue. This paper proposes a mobile scanning method aided by collimated lasers, which uses lasers as corresponding points to assist with image stitching to address the problems. Additionally, the lasers serve as structured light, enabling the measurement of image projection relationships. An inspection car was developed based on this method for the experiment. To ensure operational flexibility, a single checkerboard was used to calibrate the system, including estimating the poses of lasers and cameras, and a Laplace kernel-based algorithm was developed to guarantee the calibration accuracy. Experiments show that the performance of this algorithm exceeds that of other benchmark algorithms, and the proposed method produces nearly seamless, measurable tunnel lining images, demonstrating its feasibility. Full article
(This article belongs to the Section Remote Sensors)
Show Figures

Figure 1

11 pages, 6080 KB  
Article
Single-Shot Femtosecond Raster-Framing Imaging with High Spatio-Temporal Resolution Using Wavelength/Polarization Time Coding
by Yang Yang, Yongle Zhu, Xuanke Zeng, Dong He, Li Gu, Zhijian Wang and Jingzhen Li
Photonics 2025, 12(7), 639; https://doi.org/10.3390/photonics12070639 - 24 Jun 2025
Viewed by 1048
Abstract
This paper introduces a single-shot ultrafast imaging technique termed wavelength and polarization time-encoded ultrafast raster imaging (WP-URI). By integrating raster imaging principles with wavelength- and polarization-based temporal encoding, the system uses a spatial raster mask and time–space mapping to aggregate multiple two-dimensional temporal [...] Read more.
This paper introduces a single-shot ultrafast imaging technique termed wavelength and polarization time-encoded ultrafast raster imaging (WP-URI). By integrating raster imaging principles with wavelength- and polarization-based temporal encoding, the system uses a spatial raster mask and time–space mapping to aggregate multiple two-dimensional temporal raster images onto a single detector plane, thereby enabling the effective spatial separation and extraction of target information. Finally, the target dynamics are recovered using a reconstruction algorithm based on the Nyquist–Shannon sampling theorem. Numerical simulations demonstrate the single-shot acquisition of four dynamic frames at 25 trillion frames per second (Tfps) with an intrinsic spatial resolution of 50 line pairs per millimeter (lp/mm) and a wide field of view. The WP-URI technique achieves unparalleled spatio-temporal resolution and frame rates, offering significant potential for investigating ultrafast phenomena such as matter interactions, carrier dynamics in semiconductor devices, and femtosecond laser–matter processes. Full article
Show Figures

Figure 1

14 pages, 2289 KB  
Article
Propagation Regimes and Signal Enhancement Mechanisms of Collinear Double-Pulse Plasma with Varying Inter-Pulse Delays
by Yang Zhao, Lei Zhang, Zhihui Tian, Xiuqing Zhang, Jiandong Bai and Wangbao Yin
Sensors 2025, 25(11), 3409; https://doi.org/10.3390/s25113409 - 28 May 2025
Viewed by 1217
Abstract
Laser-induced breakdown spectroscopy (LIBS) is an in situ analytical technique. Compared to traditional single-pulse LIBS (SP-LIBS), collinear double-pulse LIBS (DP-LIBS) is a promising technique due to its lower limit of detection for trace elements. In this paper, we analyze the spectral and image [...] Read more.
Laser-induced breakdown spectroscopy (LIBS) is an in situ analytical technique. Compared to traditional single-pulse LIBS (SP-LIBS), collinear double-pulse LIBS (DP-LIBS) is a promising technique due to its lower limit of detection for trace elements. In this paper, we analyze the spectral and image information obtained from the emissions emitted by single/double pulse (SP/DP) laser-induced plasmas. The types of laser-supported absorption (LSA) waves of the plasmas were determined according to the interactions among the ablation vapor, the ambient gas, and the laser. Furthermore, the influence mechanisms of plasma shielding on DP-LIBS signal intensity enhancement with different inter-pulse delay were investigated. In our experimental conditions, the propagation regime of SP plasma is a laser-supported combustion (LSC) wave. The DP plasmas with short inter-pulse delays show the characteristics of a laser-supported detonation (LSD) wave, and the enhancement mechanism is mainly reheating for pre-plasma. On the contrary, the DP plasmas with longer inter-pulse delays show the characteristics of a LSC wave, and the increase in laser ablation is a major contributing factor to the signal improvement. In addition, the spectral lines, which are difficult to excite by SP-LIBS, can be obtained by selecting an appropriate inter-pulse delay and setting a short delay, which provides a new idea for the measurement of trace elements. Full article
(This article belongs to the Special Issue Spectral Detection Technology, Sensors and Instruments, 2nd Edition)
Show Figures

Figure 1

16 pages, 3426 KB  
Article
Maximizing Upconversion Luminescence of Co-Doped CaF₂:Yb, Er Nanoparticles at Low Laser Power for Efficient Cellular Imaging
by Neha Dubey, Sonali Gupta, Sandeep B. Shelar, K. C. Barick and Sudeshna Chandra
Molecules 2024, 29(17), 4177; https://doi.org/10.3390/molecules29174177 - 3 Sep 2024
Cited by 15 | Viewed by 4098
Abstract
Upconversion nanoparticles (UCNPs) are well-reported for bioimaging. However, their applications are limited by low luminescence intensity. To enhance the intensity, often the UCNPs are coated with macromolecules or excited with high laser power, which is detrimental to their long-term biological applications. Herein, we [...] Read more.
Upconversion nanoparticles (UCNPs) are well-reported for bioimaging. However, their applications are limited by low luminescence intensity. To enhance the intensity, often the UCNPs are coated with macromolecules or excited with high laser power, which is detrimental to their long-term biological applications. Herein, we report a novel approach to prepare co-doped CaF2:Yb3+ (20%), Er3+ with varying concentrations of Er (2%, 2.5%, 3%, and 5%) at ambient temperature with minimal surfactant and high-pressure homogenization. Strong luminescence and effective red emission of the UCNPs were seen even at low power and without functionalization. X-ray diffraction (XRD) of UCNPs revealed the formation of highly crystalline, single-phase cubic fluorite-type nanostructures, and transmission electron microscopy (TEM) showed co-doped UCNPs are of ~12 nm. The successful doping of Yb and Er was evident from TEM–energy dispersive X-ray analysis (TEM-EDAX) and X-ray photoelectron spectroscopy (XPS) studies. Photoluminescence studies of UCNPs revealed the effect of phonon coupling between host lattice (CaF2), sensitizer (Yb3+), and activator (Er3+). They exhibited tunable upconversion luminescence (UCL) under irradiation of near-infrared (NIR) light (980 nm) at low laser powers (0.28–0.7 W). The UCL properties increased until 3% doping of Er3+ ions, after which quenching of UCL was observed with higher Er3+ ion concentration, probably due to non-radiative energy transfer and cross-relaxation between Yb3+-Er3+ and Er3+-Er3+ ions. The decay studies aligned with the above observation and showed the dependence of UCL on Er3+ concentration. Further, the UCNPs exhibited strong red emission under irradiation of 980 nm light and retained their red luminescence upon internalization into cancer cell lines, as evident from confocal microscopic imaging. The present study demonstrated an effective approach to designing UCNPs with tunable luminescence properties and their capability for cellular imaging under low laser power. Full article
Show Figures

Graphical abstract

10 pages, 2607 KB  
Communication
Optical Interferometric Device for Rapid and Specific Detection of Biological Cells
by Sándor Valkai, Dániel Petrovszki, Zsombor Fáskerti, Margaréta Baumgärtner, Brigitta Biczók, Kira Dakos, Kevin Dósa, Berill B. Kirner, Anna E. Kocsis, Krisztina Nagy, István Andó and András Dér
Biosensors 2024, 14(9), 421; https://doi.org/10.3390/bios14090421 - 29 Aug 2024
Viewed by 5464
Abstract
Here, we report a rapid and accurate optical method for detecting cells from liquid samples in a label-free manner. The working principle of the method is based on the interference of parts of a conical laser beam, coming from a single-mode optical fiber [...] Read more.
Here, we report a rapid and accurate optical method for detecting cells from liquid samples in a label-free manner. The working principle of the method is based on the interference of parts of a conical laser beam, coming from a single-mode optical fiber directly, and reflected from a flat glass surface. The glass is functionalized by antibodies against the cells to be detected from the liquid sample. Cells bound to that surface modify the reflected beam, and hence, change the resulting interference pattern, too. By registering and interpreting the variation in the image, the presence of cells from the sample can be detected. As for a demonstration, cell suspensions from a U937 cell line were used in glass chambers functionalized by antibodies (TMG6-5 (mIgG1)) to which the cells specifically bind. The limit of detection (LOD) of the method was also estimated. This proof-of-concept setup offers a cost-effective and easy-to-use way of rapid and specific detection of any type of cells (including pathogens) from suspensions (e.g., body fluids). The possible portability of the device predicts its applicability as a rapid test in clinical diagnostics. Full article
(This article belongs to the Special Issue Feature Paper in Biosensor and Bioelectronic Devices 2024)
Show Figures

Figure 1

12 pages, 7509 KB  
Article
In-Situ Metabolic Profiling of Different Kinds of Rheum palmatum L. by Laser Desorption–Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging
by Xue Xiao, Xiaokang Guan, Zhouyi Xu and Qiao Lu
Metabolites 2024, 14(3), 131; https://doi.org/10.3390/metabo14030131 - 21 Feb 2024
Cited by 13 | Viewed by 2681
Abstract
With its high resolving power and sensitivity, mass spectrometry is considered the most informative technique for metabolite qualitation and quantification in the plant sciences. However, the spatial location information, which is crucial for the exploration of plant physiological mechanisms, is lost. Mass spectrometry [...] Read more.
With its high resolving power and sensitivity, mass spectrometry is considered the most informative technique for metabolite qualitation and quantification in the plant sciences. However, the spatial location information, which is crucial for the exploration of plant physiological mechanisms, is lost. Mass spectrometry imaging (MSI) is able to visualize the spatial distribution of a large number of metabolites from the complex sample surface in a single experiment. In this paper, a flexible and low-cost laser desorption–dielectric barrier discharge ionization-MSI (LD-DBDI-MSI) platform was constructed by combining an LD system with an in-line DBDI source, a high-precision sample translation stage, and an ambient mass spectrometer. It can be operated at a spatial resolution of 20 μm in an atmospheric environment and requires minimal sample preparation. This study presents images of in-situ metabolic profiling of two kinds of plants from different origins, a wild and a farmed Rheum palmatum L. From the screen of these two root sections, the wild one presented five more endogenous molecules than the farmed one, which provides information about the differences in metabolomics. Full article
Show Figures

Figure 1

11 pages, 4313 KB  
Article
Characterization of Grid Lines Formed by Laser-Induced Forward Transfer and Effect of Laser Fluence on the Silver Paste Transformation
by Yucui Yu, Yanmei Zhang, Chongxin Tian, Xiuli He, Shaoxia Li and Gang Yu
Photonics 2023, 10(7), 717; https://doi.org/10.3390/photonics10070717 - 22 Jun 2023
Cited by 4 | Viewed by 2730
Abstract
The investigation of novel approaches for forming solar cell grid lines has gained importance with the rapid development of the photovoltaic industry. Laser-induced forward transfer (LIFT) is a very promising approach for microstructure fabrication. In this work, the morphology of grid lines deposited [...] Read more.
The investigation of novel approaches for forming solar cell grid lines has gained importance with the rapid development of the photovoltaic industry. Laser-induced forward transfer (LIFT) is a very promising approach for microstructure fabrication. In this work, the morphology of grid lines deposited by LIFT was investigated. A characterization scheme for solar cell grid lines was proposed. The shape of grid lines was described, combined with confocal imaging. The evolution process of grid lines from no forming to single-peak and double-peak with a variation of laser fluence was observed. According to experimental conditions, different types of grid line morphology were obtained and transfer mechanisms of silver paste were proposed based on fluid dynamics. The influence of laser fluence on the morphology of formed grid lines was explained through phenomenology and analysis. This can provide a guide for morphology control in forming the process of grid lines. Full article
(This article belongs to the Special Issue Fiber Laser and Their Applications)
Show Figures

Figure 1

Back to TopTop