Skip to Content

13,705 Results Found

  • Article
  • Open Access
5 Citations
3,866 Views
24 Pages

Inter-Versus Intra-Host Sequence Diversity of pH1N1 and Associated Clinical Outcomes

  • Hebah A. Al Khatib,
  • Muna A. Al Maslamani,
  • Peter V. Coyle,
  • I. Richard Thompson,
  • Elmoubasher A. Farag,
  • Asmaa A. Al Thani and
  • Hadi M. Yassine

The diversity of RNA viruses dictates their evolution in a particular host, community or environment. Here, we reported within- and between-host pH1N1virus diversity at consensus and sub-consensus levels over a three-year period (2015–2017) and...

  • Article
  • Open Access
2 Citations
4,570 Views
15 Pages

31 August 2021

The study of viral diversity is imperative in understanding sequence change and its implications for intervention strategies. The widely used alignment-dependent approaches to study viral diversity are limited in their utility as sequence dissimilari...

  • Article
  • Open Access
2 Citations
2,202 Views
17 Pages

23 September 2024

The genus Tulipa L., renowned for its ornamental and ecological significance, encompasses a diversity of species primarily concentrated in the Tian Shan and Pamir-Alay Mountain ranges. With its varied landscapes, Kazakhstan harbors 42 Tulipa species,...

  • Article
  • Open Access
263 Views
7 Pages

12 January 2026

Purpose: Aeromonas species are emerging pathogens associated with a range of human infections, particularly gastroenteritis in the paediatric population. However, data on their molecular characteristics in Latvia and the wider Baltic region remain li...

  • Article
  • Open Access
908 Views
15 Pages

Genetic Diversity of a Wild Actinidia arguta Population in Changbai Mountain Determined by Simple Sequence Repeat Markers

  • Xiaowu Sun,
  • Guangli Shi,
  • Songze Li,
  • Jun Ai,
  • Dan Sun,
  • Zhenxing Wang,
  • Peijin Ni,
  • Zhendong Zhang,
  • Shuaiming Chen and
  • Fan Zhang
  • + 2 authors

With the decrease in the number of natural populations of Actinidia arguta, there is an urgent need to collect A. arguta germplasm resources and explore their genetic diversity for better management and protection. In this study, 31 simple sequence r...

  • Article
  • Open Access
1,466 Views
13 Pages

Genome-Wide Characterization and Development of Simple Sequence Repeat Markers for Molecular Diversity Analyses in Yellowhorn (Xanthoceras sorbifolium Bunge)

  • Xiaoming Yang,
  • Yuan Wang,
  • Yuewen Yang,
  • Tuya Shareng,
  • Yukun Xing,
  • Gaowa Bai,
  • Zhongyu Xing,
  • Yuanyuan Ji,
  • Liling Liu and
  • Gongxiang Cao

5 October 2024

Yellowhorn (Xanthoceras sorbifolium Bunge) is a valuable ornamental, medicinal, and woody oilseed species that is indigenous to China. The breeding improvement of yellowhorn has been hindered by a lack of suitable markers and sufficient information r...

  • Article
  • Open Access
2 Citations
2,628 Views
16 Pages

Evaluation of the HIV-1 Polymerase Gene Sequence Diversity for Prediction of Recent HIV-1 Infections Using Shannon Entropy Analysis

  • Paballo Nkone,
  • Shayne Loubser,
  • Thomas C. Quinn,
  • Andrew D. Redd,
  • Oliver Laeyendecker,
  • Caroline T. Tiemessen and
  • Simnikiwe H. Mayaphi

21 July 2022

HIV-1 incidence is an important parameter for assessing the impact of HIV-1 interventions. The aim of this study was to evaluate HIV-1 polymerase (pol) gene sequence diversity for the prediction of recent HIV-1 infections. Complete pol Sanger sequenc...

  • Article
  • Open Access
2 Citations
2,250 Views
14 Pages

22 November 2023

Huanglongbing (HLB) is a devastating citrus disease caused by Candidatus Liberibacter asiaticus (CLas). Since its initial outbreak in Guangdong Province, China, it has spread to 10 provinces and caused significant economic losses. Hence, assessing CL...

  • Article
  • Open Access
58 Citations
9,762 Views
16 Pages

22 March 2012

Rheum officinale Baill., an important but endangered medicinal herb, is endemic to China. Inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 12 populations of R. officinale. Thirteen...

  • Article
  • Open Access
1 Citations
1,745 Views
11 Pages

Cupressus gigantea W. C. Cheng & L. K. Fu is an endemic conifer tree species that is distributed widely along the northern portion of the deep gorge of the Yarlung Tsangbo River on the Tibetan Plateau. However, as a key plant species growing on t...

  • Article
  • Open Access
922 Views
16 Pages

16 July 2025

Castor fiber birulai is a subspecies of the Eurasian beaver that has a relatively small population size compared to other Castor subspecies. There is limited genetic research on this subspecies. In this study, mitochondrial cytochrome b (Cytb) and D-...

  • Article
  • Open Access
1 Citations
1,552 Views
18 Pages

Genetic Diversity Patterns Within and Among Varieties of Korean Italian Ryegrass (Lolium multiflorum) and Perennial Ryegrass (Lolium perenne) Based on Simple Sequence Repetition

  • Dong-Geon Nam,
  • Eun-Seong Baek,
  • Eun-Bin Hwang,
  • Sang-Cheol Gwak,
  • Yun-Ho Lee,
  • Seong-Woo Cho,
  • Ju-Kyung Yu and
  • Tae-Young Hwang

Italian ryegrass (Lolium multiflorum, IRG) and perennial ryegrass (Lolium perenne L., PRG) are widely cultivated as forage grasses in Korea using heterogeneous and polycross techniques, which promote genetic diversity within varieties. However, their...

  • Article
  • Open Access
37 Citations
13,673 Views
16 Pages

Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Marker Resources for Diversity Analysis of Mango (Mangifera indica L.)

  • Natalie L. Dillon,
  • David J. Innes,
  • Ian S. E. Bally,
  • Carole L. Wright,
  • Luke C. Devitt and
  • Ralf G. Dietzgen

20 January 2014

In this study, a collection of 24,840 expressed sequence tags (ESTs) generated from five mango (Mangifera indica L.) cDNA libraries was mined for EST-based simple sequence repeat (SSR) markers. Over 1,000 ESTs with SSR motifs were detected from more...

  • Article
  • Open Access
8 Citations
3,328 Views
14 Pages

Next Generation Sequencing for the Analysis of Parvovirus B19 Genomic Diversity

  • Federica Bichicchi,
  • Niccolò Guglietta,
  • Arthur Daniel Rocha Alves,
  • Erika Fasano,
  • Elisabetta Manaresi,
  • Gloria Bua and
  • Giorgio Gallinella

12 January 2023

Parvovirus B19 (B19V) is a ssDNA human virus, responsible for an ample range of clinical manifestations. Sequencing of B19V DNA from clinical samples is frequently reported in the literature to assign genotype (genotypes 1–3) and for finer mole...

  • Article
  • Open Access
88 Citations
28,129 Views
16 Pages

Genotyping-By-Sequencing for Plant Genetic Diversity Analysis: A Lab Guide for SNP Genotyping

  • Gregory W. Peterson,
  • Yibo Dong,
  • Carolee Horbach and
  • Yong-Bi Fu

20 October 2014

Genotyping-by-sequencing (GBS) has recently emerged as a promising genomic approach for exploring plant genetic diversity on a genome-wide scale. However, many uncertainties and challenges remain in the application of GBS, particularly in non-model s...

  • Article
  • Open Access
6 Citations
3,180 Views
13 Pages

19 December 2022

Tau aggregation associates with multiple neurodegenerative diseases including Alzheimer’s disease and rare tauopathies such as Pick’s disease, progressive supranuclear palsy, and corticobasal degeneration. The molecular and structural bas...

  • Article
  • Open Access
11 Citations
2,966 Views
23 Pages

Characterization of Microbial Diversity of Two Tomato Cultivars through Targeted Next-Generation Sequencing 16S rRNA and ITS Techniques

  • Rukayat Abiola Abdulsalam,
  • Oluwatosin Ademola Ijabadeniyi,
  • Errol D. Cason and
  • Saheed Sabiu

Even though the nutritional and economic values of Solanum lycopersicum (tomato) are substantially impacted by microbial spoilage, the available data on its microbial community, particularly during spoilage, are limited and have primarily been charac...

  • Article
  • Open Access
14 Citations
3,040 Views
19 Pages

Baijiu is a traditional distilled liquor in China. The unique flavor developed during the fermentation process of Baijiu is closely related to the microorganisms in the fermented grains of the Baijiu. Fermented grain is a solid material that has not...

  • Article
  • Open Access
13 Citations
6,083 Views
13 Pages

Genetic Diversity Assessed by Genotyping by Sequencing (GBS) in Watermelon Germplasm

  • Kyung Jun Lee,
  • Jung-Ro Lee,
  • Raveendar Sebastin,
  • Myoung-Jae Shin,
  • Seong-Hoon Kim,
  • Gyu-Taek Cho and
  • Do Yoon Hyun

18 October 2019

Watermelon is an economically important vegetable fruit worldwide. The objective of this study was to conduct a genetic diversity of 68 watermelon accessions using single nucleotide polymorphisms (SNPs). Genotyping by sequencing (GBS) was used to dis...

  • Article
  • Open Access
23 Citations
7,136 Views
18 Pages

Population Structure and Diversity in European Honey Bees (Apis mellifera L.)—An Empirical Comparison of Pool and Individual Whole-Genome Sequencing

  • Chao Chen,
  • Melanie Parejo,
  • Jamal Momeni,
  • Jorge Langa,
  • Rasmus O. Nielsen,
  • Wei Shi,
  • SMARTBEES WP3 DIVERSITY CONTRIBUTORS,
  • Rikke Vingborg,
  • Per Kryger and
  • Marina Meixner
  • + 2 authors

21 January 2022

Background: Whole-genome sequencing has become routine for population genetic studies. Sequencing of individuals provides maximal data but is rather expensive and fewer samples can be studied. In contrast, sequencing a pool of samples (pool-seq) can...

  • Article
  • Open Access
20 Citations
6,985 Views
15 Pages

High-Throughput Sequencing Analysis of the Actinobacterial Spatial Diversity in Moonmilk Deposits

  • Marta Maciejewska,
  • Magdalena Całusińska,
  • Luc Cornet,
  • Delphine Adam,
  • Igor S. Pessi,
  • Sandrine Malchair,
  • Philippe Delfosse,
  • Denis Baurain,
  • Hazel A. Barton and
  • Sébastien Rigali
  • + 1 author

Moonmilk are cave carbonate deposits that host a rich microbiome, including antibiotic-producing Actinobacteria, making these speleothems appealing for bioprospecting. Here, we investigated the taxonomic profile of the actinobacterial community of th...

  • Review
  • Open Access
1 Citations
1,385 Views
20 Pages

11 October 2025

Parasites of the Leishmania genus are globally distributed and cause various clinical presentations in animals and humans, collectively known as leishmaniasis. The genomes of Leishmania and other trypanosomatids exhibit remarkable plasticity, shaped...

  • Article
  • Open Access
4 Citations
7,133 Views
14 Pages

Genetic Diversity of Northern Wheatgrass (Elymus lanceolatus ssp. lanceolatus) as Revealed by Genotyping-by-Sequencing

  • Pingchuan Li,
  • Surendra Bhattarai,
  • Gregory W. Peterson,
  • Bruce Coulman,
  • Michael P. Schellenberg,
  • Bill Biligetu and
  • Yong-Bi Fu

11 April 2018

Recent advances in next generation sequencing technologies make genotyping-by-sequencing (GBS) more feasible for the molecular characterization of plant germplasm with complex and unsequenced genomes. This study represents the first preliminary effor...

  • Article
  • Open Access
6 Citations
2,725 Views
12 Pages

Genetic Diversity and Population Structure of Coilia nasus Revealed by 2b-RAD Sequencing

  • Shuangmeng Zhang,
  • Zisheng Xu,
  • Lifei Luo,
  • Shuxin Gu,
  • Zhen Hu,
  • Shiming Wan and
  • Zexia Gao

14 April 2023

Coilia nasus is a threatened migratory species in the Yangtze River Basin. To reveal the genetic diversity of natural and farmed populations of C. nasus and the status of germplasm resources in the Yangtze River, the genetic diversity and structure o...

  • Article
  • Open Access
11 Citations
5,335 Views
14 Pages

Genotyping-By-Sequencing Reveals Population Structure and Genetic Diversity of a Buffelgrass (Cenchrus ciliaris L.) Collection

  • Alemayehu Teressa Negawo,
  • Yilikal Assefa,
  • Jean Hanson,
  • Asebe Abdena,
  • Meki S. Muktar,
  • Ermias Habte,
  • Alieu M. Sartie and
  • Chris S. Jones

27 February 2020

Buffelgrass (Cenchrus ciliaris L.) is an important forage grass widely grown across the world with many good characteristics including high biomass yield, drought tolerance, and adaptability to a wide range of soil conditions and agro-ecologies. Two...

  • Article
  • Open Access
12 Citations
5,258 Views
11 Pages

21 February 2019

The origins of life require the emergence of informational polymers capable of reproduction. In the RNA world on the primordial Earth, reproducible RNA molecules would have arisen from a mixture of compositionally biased, poorly available, short RNA...

  • Article
  • Open Access
258 Views
11 Pages

16 January 2026

Whole-genome sequencing is a powerful approach for exploring genomic diversity in livestock species. Chickens (Gallus gallus) are an important food source worldwide, and in Taiwan, poultry production contributes substantially to the livestock industr...

  • Article
  • Open Access
14 Citations
4,748 Views
17 Pages

Genetic Diversity, Population Structure and Selection Signature in Begait Goats Revealed by Whole-Genome Sequencing

  • Haile Berihulay Gebreselase,
  • Hailemichael Nigussie,
  • Changfa Wang and
  • Chenglong Luo

18 January 2024

Goats belong to a group of animals called small ruminants and are critical sources of livelihood for rural people. Genomic sequencing can provide information ranging from basic knowledge about goat diversity and evolutionary processes that shape geno...

  • Article
  • Open Access
18 Citations
4,984 Views
13 Pages

Molecular characterization of unsequenced plant species with complex genomes is now possible by genotyping-by-sequencing (GBS) using recent next generation sequencing technologies. This study represents the first use of GBS application to sample geno...

  • Article
  • Open Access
4 Citations
3,533 Views
12 Pages

8 January 2021

Phylodynamic inference is a pivotal tool in understanding transmission dynamics of viral outbreaks. These analyses are strongly guided by the input of an epidemiological model as well as sequence data that must contain sufficient intersequence variab...

  • Article
  • Open Access
14 Citations
4,936 Views
24 Pages

12 June 2020

Research Highlights: This study, based on the first collection of cultivated Paeonia rockii (flare tree peony, FTP) germplasm across the main distribution area by our breeding desires, comprehensively evaluates these accessions by using phenotypic tr...

  • Article
  • Open Access
8 Citations
3,614 Views
12 Pages

Genetic Diversity and Population Structure Analysis of Hollyhock (Alcea rosea Cavan) Using High-Throughput Sequencing

  • Yue Wang,
  • Sheng Zhao,
  • Peng Chen,
  • Yongkang Liu,
  • Zhigang Ma,
  • Waqar Afzal Malik,
  • Zhenghang Zhu,
  • Zhenyu Peng,
  • Haorong Lu and
  • Yuxiao Chang
  • + 1 author

Hollyhock (Alcea rosea (Linn). Cavan) is an herbaceous flowering plant with significant applications in urban greening, soil remediation, and traditional medicine. However, its genetic diversity and molecular characteristics at the population level h...

  • Article
  • Open Access
5 Citations
3,152 Views
13 Pages

In this study, high-throughput sequencing technology was used to analyze the bacterial diversity of sauerkraut produced at home and in factories in Chaozhou. The differences in bacterial community structure among different sauerkraut samples were stu...

  • Article
  • Open Access
1 Citations
2,139 Views
20 Pages

Genetic Diversity of Olive (Olea europaea L.) Cultivars Assessed by Genotyping-by-Sequencing in Southern Peru

  • Martín Eloy Casilla García,
  • Rina Alvarez Becerra,
  • José Cotrado Cotrado,
  • Juan Iván Casilla Rondán,
  • Janet Libertad Huatuco Coaquira and
  • Edgar Virgilio Bedoya Justo

The genetic diversity of the olive tree (Olea europaea L.) is critical for enhancing crop resilience and productivity under changing climatic conditions. Peru’s southern region, particularly Tacna, hosts over 30 olive cultivars, yet their genet...

  • Article
  • Open Access
5 Citations
4,007 Views
16 Pages

Genome-Wide Genetic Diversity and Population Structure of Local Sudanese Sheep Populations Revealed by Whole-Genome Sequencing

  • Agraw Amane,
  • Gurja Belay,
  • Abdulfatai Tijjani,
  • Tadelle Dessie,
  • Hassan H. Musa and
  • Olivier Hanotte

22 October 2022

Local Sudanese sheep populations inhabiting diverse environmental conditions and holding opposing morphologies provide opportunities for molecular-genetic research. Characterizing their genome is crucial for sustainable breeding improvement and targe...

  • Article
  • Open Access
1,121 Views
13 Pages

Guava (Psidium guajava L.), a perennial species native to tropical regions of the Americas, holds significant economic value and plays an important role in the global fruit industry. Although several reference genomes have been published, population-...

  • Article
  • Open Access
15 Citations
3,574 Views
18 Pages

28 February 2023

The accumulation of biowastes is one of the main concerns of modern society. One of the most environmentally friendly solutions to convert biowaste into a product is composting. Biowastes may contain unknown substances that are persistent in the fina...

  • Article
  • Open Access
3 Citations
1,591 Views
13 Pages

23 May 2024

Tsoongiodendron odorum Chun is a large evergreen tree in the Magnoliaceae family and an ancient relict species represented by small wild populations. It has excellent material quality, high ornamental value, and scientific significance. However, due...

  • Article
  • Open Access
11 Citations
3,218 Views
13 Pages

Employing Genome-Wide SNP Discovery to Characterize the Genetic Diversity in Cinnamomum camphora Using Genotyping by Sequencing

  • Xue Gong,
  • Aihong Yang,
  • Zhaoxiang Wu,
  • Caihui Chen,
  • Huihu Li,
  • Qiaoli Liu,
  • Faxin Yu and
  • Yongda Zhong

1 November 2021

Cinnamomum camphora (L.) J.Presl is a representative tree species of evergreen broad-leafed forests in East Asia and has exceptionally high economic, ornamental, and ecological value. However, the excessive exploitation and utilization of C. camphora...

  • Communication
  • Open Access
3 Citations
5,366 Views
9 Pages

Using Next Generation Sequencing to Study the Genetic Diversity of Candidate Live Attenuated Zika Vaccines

  • Natalie D. Collins,
  • Chao Shan,
  • Bruno T.D. Nunes,
  • Steven G. Widen,
  • Pei-Yong Shi,
  • Alan D.T. Barrett and
  • Vanessa V. Sarathy

Zika virus (ZIKV) is a mosquito-transmitted positive-sense RNA virus in the family Flaviviridae. Candidate live-attenuated vaccine (LAV) viruses with engineered deletions in the 3’ untranslated region (UTR) provide immunity and protection in an...

  • Article
  • Open Access
609 Views
20 Pages

28 November 2025

This study conducted a comprehensive survey of macrofungal diversity in the Niyang River Basin by integrating soil high-throughput sequencing (HTS) with a traditional field investigation (TFI), aiming to evaluate the potential species pool and to ass...

  • Article
  • Open Access
6 Citations
3,605 Views
23 Pages

1 September 2022

Eukaryotes exist widely in aquatic ecosystems. It is of great importance to study their species composition, diversity, and relationship with environmental factors to protect and maintain ecosystem balance. Salt lakes are essential lakes rich in biol...

  • Article
  • Open Access
6 Citations
4,425 Views
18 Pages

Molecular Diversity of Peptide Toxins in the Venom of Spider Heteropoda pingtungensis as Revealed by cDNA Library and Transcriptome Sequencing Analysis

  • Qingyi Liao,
  • Xiangjin Kong,
  • Guoqing Luo,
  • Xiangyue Wu,
  • Yinping Li,
  • Qicai Liu,
  • Cheng Tang and
  • Zhonghua Liu

14 February 2022

The venoms of toxic animals are chemical pools composed of various proteins, peptides, and small organic molecules used for predation and defense, in which the peptidic toxins have been intensively pursued mining modulators targeting disease-related...

  • Article
  • Open Access
15 Citations
4,018 Views
14 Pages

Evaluation of the Genetic Diversity and Differentiation of Black Locust (Robinia pseudoacacia L.) Based on Genomic and Expressed Sequence Tag-Simple Sequence Repeats

  • Qi Guo,
  • Xiuyu Li,
  • Shuhong Yang,
  • Zhiheng Yang,
  • Yuhan Sun,
  • Jiangtao Zhang,
  • Sen Cao,
  • Li Dong,
  • Saleem Uddin and
  • Yun Li

Understanding the genetic diversity and differentiation of the genetic resources of a species is important for the effective use and protection of forest tree resources. Ex situ development is a common method for the protection of genetic diversity a...

  • Article
  • Open Access
935 Views
17 Pages

High-Throughput Sequencing Reveals the Mycoviral Diversity of the Pathogenic Grape Fungus Penicillium astrolabium During Postharvest

  • Rui Wang,
  • Guoqin Wen,
  • Xiaohong Liu,
  • Yingqing Luo,
  • Yanhua Chang,
  • Guoqi Li and
  • Tingfu Zhang

28 July 2025

Penicillium astrolabium is a primary pathogenic fungus that causes grape blue mold during postharvest, leading to substantial losses in the grape industry. Nevertheless, hypovirulence-associated mycoviruses can attenuate the virulence of postharvest...

  • Article
  • Open Access
3 Citations
1,935 Views
23 Pages

Genetic Diversity of Grapevine Virus A in Three Australian Vineyards Using Amplicon High Throughput Sequencing (Amplicon-HTS)

  • Qi Wu,
  • Wycliff M. Kinoti,
  • Nuredin Habili,
  • Stephen D. Tyerman,
  • Amy Rinaldo and
  • Fiona E. Constable

27 December 2023

Shiraz disease (SD) is one of the most destructive viral diseases of grapevines in Australia and is known to cause significant economic loss to local growers. Grapevine virus A (GVA) was reported to be the key pathogen associated with this disease. T...

  • Article
  • Open Access
3 Citations
1,867 Views
12 Pages

3 September 2024

Head-to-tail sequences have been reported in human bocavirus (HBoV) 1-4. To reveal their features and functions, HBoV DNA was screened among respiratory specimens from pediatric patients with an acute respiratory infection (ARI) between April 2020 an...

  • Article
  • Open Access
11 Citations
4,276 Views
14 Pages

11 July 2020

Research on the ecology and evolution of viruses is often hampered by the limitation of sequence information to short parts of the genomes or single genomes derived from cultures. In this study, we use hybrid sequence capture enrichment in combinatio...

  • Review
  • Open Access
144 Citations
17,251 Views
24 Pages

10 November 2009

Evaluation of the genetic diversity among populations is an essential prerequisite for the preservation of endangered species. Thousands of new accessions are introduced into germplasm institutes each year, thereby necessitating assessment of their m...

  • Article
  • Open Access
1 Citations
1,984 Views
14 Pages

30 August 2023

Zanthoxylum (Sichuan pepper), with its rich cultivars, has long been widely cultivated in China for its unique seasoning and medicinal uses, but most of its cultivars have similar morphological characteristics. Therefore, we hypothesized that the gen...

of 275