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26 pages, 6698 KiB  
Article
Cumulative and Lagged Effects of Drought on the Phenology of Different Vegetation Types in East Asia, 2001–2020
by Kexin Deng, Mark Henderson, Binhui Liu, Weiwei Huang, Mingyang Chen, Pingping Zheng and Ruiting Gu
Remote Sens. 2025, 17(15), 2700; https://doi.org/10.3390/rs17152700 - 4 Aug 2025
Abstract
Drought disturbances are becoming more frequent with global warming. Accurately assessing the regulatory effect of drought on vegetation phenology is key to understanding terrestrial ecosystem response mechanisms in the context of climate change. Previous studies on cumulative and lagged effects of drought on [...] Read more.
Drought disturbances are becoming more frequent with global warming. Accurately assessing the regulatory effect of drought on vegetation phenology is key to understanding terrestrial ecosystem response mechanisms in the context of climate change. Previous studies on cumulative and lagged effects of drought on vegetation growth have mostly focused on a single vegetation type or the overall vegetation NDVI, overlooking the possible influence of different adaptation strategies of different vegetation types and differences in drought effects on different phenological nodes. This study investigates the cumulative and lagged effects of drought on vegetation phenology across a region of East Asia from 2001 to 2020 using NDVI data and the Standardized Precipitation Evapotranspiration Index (SPEI). We analyzed the start of growing season (SOS) and end of growing season (EOS) responses to drought across four vegetation types: deciduous needleleaf forests (DNFs), deciduous broadleaf forests (DBFs), shrublands, and grasslands. Results reveal contrasting phenological responses: drought delayed SOS in grasslands through a “drought escape” strategy but advanced SOS in forests and shrublands. All vegetation types showed earlier EOS under drought stress. Cumulative drought effects were strongest on DNFs, SOS, and shrubland SOS, while lagged effects dominated DBFs and grassland SOS. Drought impacts varied with moisture conditions: they were stronger in dry regions for SOS but more pronounced in humid areas for EOS. By confirming that drought effects vary by vegetation type and phenology node, these findings enhance our understanding of vegetation adaptation strategies and ecosystem responses to climate stress. Full article
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25 pages, 10827 KiB  
Article
Integrated Transcriptomic and Metabolomic Analysis Reveals Nitrogen-Mediated Delay of Premature Leaf Senescence in Red Raspberry Leaves
by Qiang Huo, Feiyang Chang, Peng Jia, Ziqian Fu, Jiaqi Zhao, Yiwen Gao, Haoan Luan, Ying Wang, Qinglong Dong, Guohui Qi and Xuemei Zhang
Plants 2025, 14(15), 2388; https://doi.org/10.3390/plants14152388 - 2 Aug 2025
Viewed by 245
Abstract
The premature senescence of red raspberry leaves severely affects plant growth. In this study, the double-season red raspberry cultivar ‘Polka’ was used, with N150 (0.10 g N·kg−1) selected as the treatment group (T150) and N0 (0 g N·kg−1 [...] Read more.
The premature senescence of red raspberry leaves severely affects plant growth. In this study, the double-season red raspberry cultivar ‘Polka’ was used, with N150 (0.10 g N·kg−1) selected as the treatment group (T150) and N0 (0 g N·kg−1) set as the control (CK). This study systematically investigated the mechanism of premature senescence in red raspberry leaves under different nitrogen application levels by measuring physiological parameters and conducting a combined multi-omics analysis of transcriptomics and metabolomics. Results showed that T150 plants had 8.34 cm greater height and 1.45 cm greater ground diameter than CK. The chlorophyll, carotenoid, soluble protein, and sugar contents in all leaf parts of T150 were significantly higher than those in CK, whereas soluble starch contents were lower. Malondialdehyde (MDA) content and superoxide anion (O2) generation rate in the lower leaves of T150 were significantly lower than those in CK. Superoxide sismutase (SOD) and peroxidase (POD) activities in the middle and lower functional leaves of T150 were higher than in CK, while catalase (CAT) activity was lower. Transcriptomic analysis identified 4350 significantly differentially expressed genes, including 2062 upregulated and 2288 downregulated genes. Metabolomic analysis identified 135 differential metabolites, out of which 60 were upregulated and 75 were downregulated. Integrated transcriptomic and metabolomic analysis showed enrichment in the phenylpropanoid biosynthesis (ko00940) and flavonoid biosynthesis (ko00941) pathways, with the former acting as an upstream pathway of the latter. A premature senescence pathway was established, and two key metabolites were identified: chlorogenic acid content decreased, and naringenin chalcone content increased in early senescent leaves, suggesting their pivotal roles in the early senescence of red raspberry leaves. Modulating chlorogenic acid and naringenin chalcone levels could delay premature senescence. Optimizing fertilization strategies may thus reduce senescence risk and enhance the productivity, profitability, and sustainability of the red raspberry industry. Full article
(This article belongs to the Special Issue Horticultural Plant Physiology and Molecular Biology)
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29 pages, 1289 KiB  
Article
An Analysis of Hybrid Management Strategies for Addressing Passenger Injuries and Equipment Failures in the Taipei Metro System: Enhancing Operational Quality and Resilience
by Sung-Neng Peng, Chien-Yi Huang, Hwa-Dong Liu and Ping-Jui Lin
Mathematics 2025, 13(15), 2470; https://doi.org/10.3390/math13152470 - 31 Jul 2025
Viewed by 282
Abstract
This study is the first to systematically integrate supervised machine learning (decision tree) and association rule mining techniques to analyze accident data from the Taipei Metro system, conducting a large-scale data-driven investigation into both passenger injury and train malfunction events. The research demonstrates [...] Read more.
This study is the first to systematically integrate supervised machine learning (decision tree) and association rule mining techniques to analyze accident data from the Taipei Metro system, conducting a large-scale data-driven investigation into both passenger injury and train malfunction events. The research demonstrates strong novelty and practical contributions. In the passenger injury analysis, a dataset of 3331 cases was examined, from which two highly explanatory rules were extracted: (i) elderly passengers (aged > 61) involved in station incidents are more likely to suffer moderate to severe injuries; and (ii) younger passengers (aged ≤ 61) involved in escalator incidents during off-peak hours are also at higher risk of severe injury. This is the first study to quantitatively reveal the interactive effect of age and time of use on injury severity. In the train malfunction analysis, 1157 incidents with delays exceeding five minutes were analyzed. The study identified high-risk condition combinations—such as those involving rolling stock, power supply, communication, and signaling systems—associated with specific seasons and time periods (e.g., a lift value of 4.0 for power system failures during clear mornings from 06:00–12:00, and 3.27 for communication failures during summer evenings from 18:00–24:00). These findings were further cross-validated with maintenance records to uncover underlying causes, including brake system failures, cable aging, and automatic train operation (ATO) module malfunctions. Targeted preventive maintenance recommendations were proposed. Additionally, the study highlighted existing gaps in the completeness and consistency of maintenance records, recommending improvements in documentation standards and data auditing mechanisms. Overall, this research presents a new paradigm for intelligent metro system maintenance and safety prediction, offering substantial potential for broader adoption and practical application. Full article
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25 pages, 4161 KiB  
Article
Indoor/Outdoor Particulate Matter and Related Pollutants in a Sensitive Public Building in Madrid (Spain)
by Elisabeth Alonso-Blanco, Francisco Javier Gómez-Moreno, Elías Díaz-Ramiro, Javier Fernández, Esther Coz, Carlos Yagüe, Carlos Román-Cascón, Dulcenombre Gómez-Garre, Adolfo Narros, Rafael Borge and Begoña Artíñano
Int. J. Environ. Res. Public Health 2025, 22(8), 1175; https://doi.org/10.3390/ijerph22081175 - 25 Jul 2025
Viewed by 372
Abstract
According to the World Health Organization (WHO), indoor air quality (IAQ) is becoming a serious global concern due to its significant impact on human health. However, not all relevant health parameters are currently regulated. For example, particle number concentration (PNC) and its associated [...] Read more.
According to the World Health Organization (WHO), indoor air quality (IAQ) is becoming a serious global concern due to its significant impact on human health. However, not all relevant health parameters are currently regulated. For example, particle number concentration (PNC) and its associated carbonaceous species, such as black carbon (BC), which are classified as carcinogenic by the International Agency for Research on Cancer (IARC), are not currently regulated. Compared with IAQ studies in other types of buildings, studies focusing on IAQ in hospitals or other healthcare facilities are scarce. Therefore, this study aims to evaluate the impact of these outdoor pollutants, among others, on the indoor environment of a hospital under different atmospheric conditions. To identify the seasonal influence, two different periods of two consecutive seasons (summer 2020 and winter 2021) were selected for the measurements. Regulated pollutants (NO, NO2, O3, PM10, and PM2.5) and nonregulated pollutants (PM1, PNC, and equivalent BC (eBC)) in outdoor air were simultaneously measured indoor and outdoor. This study also investigated the impact of indoor activities on indoor air quality. In the absence of indoor activities, outdoor sources significantly contribute to indoor traffic-related pollutants. Indoor and outdoor (I-O) measurements showed similar behavior, but indoor concentrations were lower, with peak levels delayed by up to two hours. Seasonal variations in indoor/outdoor (I/O) ratios were lower for particles than for associated gaseous pollutants. Particle infiltration depended on particle size, with it being higher the smaller the particle size. Indoor activities also significantly affected indoor pollutants. PMx (especially PM10 and PM2.5) concentrations were mainly modulated by walking-induced particle resuspension. Vertical eBC profiles indicated a relatively well-mixed environment. Ventilation through open windows rapidly altered indoor air quality. Outdoor-dominant pollutants (PNC, eBC, and NOX) had I/O ratios ≥ 1. Staying in the room with an open window had a synergistic effect, increasing the I/O ratios for all pollutants. Higher I/O ratios were associated with turbulent outdoor conditions in both unoccupied and occupied conditions. Statistically significant differences were observed between stable (TKE ≤ 1 m2 s−2) and unstable (TKE > 1 m2 s−2) conditions, except for NO2 in summer. This finding was particularly significant when the wind direction was westerly or easterly during unstable conditions. The results of this study highlight the importance of understanding the behavior of indoor particulate matter and related pollutants. These pollutants are highly variable, and knowledge about them is crucial for determining their health effects, particularly in public buildings such as hospitals, where information on IAQ is often limited. More measurement data is particularly important for further research into I-O transport mechanisms, which are essential for developing preventive measures and improving IAQ. Full article
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22 pages, 658 KiB  
Article
Integrating Cultivation Practices and Post-Emergence Herbicides for ALS-Resistant False Cleavers (Galium spurium L.) Management in Durum Wheat
by Panagiotis Sparangis, Aspasia Efthimiadou, Nikolaos Katsenios, Kyriakos D. Giannoulis and Anestis Karkanis
Agronomy 2025, 15(8), 1786; https://doi.org/10.3390/agronomy15081786 - 24 Jul 2025
Viewed by 685
Abstract
False cleavers (Galium spurium L.) is a broadleaf weed species that affects wheat productivity because of its strong competition for resources. It has developed resistance to acetolactate synthase (ALS) inhibitors, such as sulfonylureas and triazolopyrimidines, which are herbicides widely used in durum [...] Read more.
False cleavers (Galium spurium L.) is a broadleaf weed species that affects wheat productivity because of its strong competition for resources. It has developed resistance to acetolactate synthase (ALS) inhibitors, such as sulfonylureas and triazolopyrimidines, which are herbicides widely used in durum wheat. Integrated weed management programs can contribute to the control of this species and delay the evolution of herbicide resistance. Thus, a two-year field experiment was conducted to evaluate the effects of sowing time, variety, and herbicides on crop yield, density, and dry weight of a false cleavers population with resistance to ALS inhibitors. In both growing seasons, a split-split-plot design was used with three replicates. The sowing date was chosen as the main plot factor, durum wheat varieties as the subplot factor, and herbicides as the sub-subplot factor. The herbicide treatments were: (1) metsulfuron-methyl/bensulfuron-methyl (4/50 g a.i. ha−1), (2) aminopyralid/florasulam (9.9/4.95 g a.i. ha−1), (3) pyroxsulam and florasulam/2,4-D (18.75 + 4.725/225 g a.i. ha−1), (4) 2,4-D/bromoxynil (633.15/601.2 g a.i. ha−1), non-treated control, and hand-weeded control for the first season, while in the second season one more herbicide treatment (halauxifen-methyl/florasulam, 5.6/5.15 g a.i. ha−1) was added. Herbicide application was performed on 10 March 2021 and 28 March 2022, when the crop was at the end of tillering and the beginning of stem elongation. The results showed that the density of false cleavers was not affected by the variety or sowing time. However, its dry weight was 17.3–23.4% higher in early sowing (16 November in 2020 and 8 November 2021) than in late sowing (24 December 2020 and 2 December 2021). Among the herbicides tested, 2,4-D/bromoxynil and halauxifen-methyl/florasulam effectively controlled false cleavers, showing greater efficacy in late sowing (>88%), which ultimately led to a higher yield. In conclusion, our two-year findings demonstrate that delayed sowing as part of an integrated weed management strategy can contribute to controlling resistant populations of false cleavers to ALS-inhibiting herbicides without affecting the quantity and quality of durum wheat yield in areas with a Mediterranean climate. Full article
(This article belongs to the Special Issue Weed Biology and Ecology: Importance to Integrated Weed Management)
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20 pages, 5571 KiB  
Proceeding Paper
A Forecasting Method Based on a Dynamical Approach and Time Series Data for Vehicle Service Parts Demand
by Vinh Long Phan, Makoto Taniguchi and Hidenori Yabushita
Eng. Proc. 2025, 101(1), 3; https://doi.org/10.3390/engproc2025101003 - 21 Jul 2025
Viewed by 179
Abstract
In the automotive industry, the supply of service parts—such as bumpers, batteries, and aero parts—is required even after the end of vehicle production, as customers need them for maintenance and repairs. To earn customer confidence, manufacturers must ensure timely availability of these parts [...] Read more.
In the automotive industry, the supply of service parts—such as bumpers, batteries, and aero parts—is required even after the end of vehicle production, as customers need them for maintenance and repairs. To earn customer confidence, manufacturers must ensure timely availability of these parts while managing inventory efficiently. An excess of inventory can increase warehousing costs, while stock shortages can lead to supply delays. Accurate demand forecasting is essential to balance these factors, considering the changing demand characteristics over time, such as long-term trends, seasonal fluctuations, and irregular variations. This paper introduces a novel method for time series forecasting that employs Ensemble Empirical Mode Decomposition (EEMD) and Dynamic Mode Decomposition (DMD) to analyze service part demand. EEMD decomposes historical order data into multiple modes, and DMD is used to predict transitions within these modes. The proposed method demonstrated an approximately 30% reduction in forecasting error compared to comparative methods, showcasing its effectiveness in accurately predicting service parts demand across various patterns. Full article
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16 pages, 3372 KiB  
Article
Monitoring the Time-Lagged Response of Land Subsidence to Groundwater Fluctuations via InSAR and Distributed Fiber-Optic Strain Sensing
by Qing He, Hehe Liu, Lu Wei, Jing Ding, Heling Sun and Zhen Zhang
Appl. Sci. 2025, 15(14), 7991; https://doi.org/10.3390/app15147991 - 17 Jul 2025
Viewed by 302
Abstract
Understanding the time-lagged response of land subsidence to groundwater level fluctuations and subsurface strain variations is crucial for uncovering its underlying mechanisms and enhancing disaster early warning capabilities. This study focuses on Dangshan County, Anhui Province, China, and systematically analyzes the spatio-temporal evolution [...] Read more.
Understanding the time-lagged response of land subsidence to groundwater level fluctuations and subsurface strain variations is crucial for uncovering its underlying mechanisms and enhancing disaster early warning capabilities. This study focuses on Dangshan County, Anhui Province, China, and systematically analyzes the spatio-temporal evolution of land subsidence from 2018 to 2024. A total of 207 Sentinel-1 SAR images were first processed using the Small Baseline Subset Interferometric Synthetic Aperture Radar (SBAS-InSAR) technique to generate high-resolution surface deformation time series. Subsequently, the seasonal-trend decomposition using the LOESS (STL) model was applied to extract annual cyclic deformation components from the InSAR-derived time series. To quantitatively assess the delayed response of land subsidence to groundwater level changes and subsurface strain evolution, time-lagged cross-correlation (TLCC) analysis was performed between surface deformation and both groundwater level data and distributed fiber-optic strain measurements within the 5–50 m depth interval. The strain data was collected using a borehole-based automated distributed fiber-optic sensing system. The results indicate that land subsidence is primarily concentrated in the urban core, with annual cyclic amplitudes ranging from 10 to 18 mm and peak values reaching 22 mm. The timing of surface rebound shows spatial variability, typically occurring in mid-February in residential areas and mid-May in agricultural zones. The analysis reveals that surface deformation lags behind groundwater fluctuations by approximately 2 to 3 months, depending on local hydrogeological conditions, while subsurface strain changes generally lead surface subsidence by about 3 months. These findings demonstrate the strong predictive potential of distributed fiber-optic sensing in capturing precursory deformation signals and underscore the importance of integrating InSAR, hydrological, and geotechnical data for advancing the understanding of subsidence mechanisms and improving monitoring and mitigation efforts. Full article
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13 pages, 1476 KiB  
Article
Interactive Effects of Ambient Ozone and Meteorological Factors on Cerebral Infarction: A Five-Year Time-Series Study
by Yanzhe Chen, Songtai Yang, Hanya Que, Jiamin Liu, Zhe Wang, Na Wang, Yunkun Qin, Meng Li and Fang Zhou
Toxics 2025, 13(7), 598; https://doi.org/10.3390/toxics13070598 - 16 Jul 2025
Viewed by 345
Abstract
Objective: Our objective was to investigate the short-term effects of ambient ozone (O3) meteorological factors and their interactions on hospitalizations for cerebral infarction in Zhengzhou, China. Methods: Daily data on air pollutants, meteorological factors, and hospitalization of cerebral infarction patients [...] Read more.
Objective: Our objective was to investigate the short-term effects of ambient ozone (O3) meteorological factors and their interactions on hospitalizations for cerebral infarction in Zhengzhou, China. Methods: Daily data on air pollutants, meteorological factors, and hospitalization of cerebral infarction patients were collected from 1 January 2019 to 31 December 2023 in Zhengzhou, China. A generalized additive model was constructed to evaluate the association between ambient O3 levels and hospitalization for cerebral infarction. A distributed lag non-linear model was applied to capture lagged and non-linear exposure effects. We further examined the modifying roles of temperature, humidity, wind speed, and atmospheric pressure, and conducted stratified analyses by sex, age, and season. Results: O3 exposure was significantly associated with increased cerebral infarction risk, particularly during the warm season. A bimodal temperature-lag pattern was observed, as follows: moderate temperatures (10–20 °C) were associated with immediate effects, while cold (<10 °C) and hot (>30 °C) temperatures were linked to delayed risks. The association of O3 and hospitalizations for cerebral infarction appeared stronger under high humidity, low wind speed, and low atmospheric pressure. Conclusions: Short-term O3 exposure and adverse meteorological conditions are jointly associated with an elevated risk of cerebral infarction. Integrated air quality and weather-based warning systems are essential for targeted stroke prevention. Full article
(This article belongs to the Special Issue Ozone Pollution and Adverse Health Impacts)
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23 pages, 8407 KiB  
Article
Assessing the Combined Influence of Indoor Air Quality and Visitor Flow Toward Preventive Conservation at the Peggy Guggenheim Collection
by Maria Catrambone, Emiliano Cristiani, Cristiano Riminesi, Elia Onofri and Luciano Pensabene Buemi
Atmosphere 2025, 16(7), 860; https://doi.org/10.3390/atmos16070860 - 15 Jul 2025
Viewed by 368
Abstract
The study at the Peggy Guggenheim Collection in Venice highlights critical interactions between indoor air quality, visitor dynamics, and microclimatic conditions, offering insights into preventive conservation of modern artworks. By analyzing pollutants such as ammonia, formaldehyde, and organic acids, alongside visitor density and [...] Read more.
The study at the Peggy Guggenheim Collection in Venice highlights critical interactions between indoor air quality, visitor dynamics, and microclimatic conditions, offering insights into preventive conservation of modern artworks. By analyzing pollutants such as ammonia, formaldehyde, and organic acids, alongside visitor density and environmental data, the research identified key patterns and risks. Through three seasonal monitoring campaigns, the concentrations of SO2 (sulphur dioxide), NO (nitric oxide), NO2 (nitrogen dioxide), NOx (nitrogen oxides), HONO (nitrous acid), HNO3 (nitric acid), O3 (ozone), NH3 (ammonia), CH3COOH (acetic acid), HCOOH (formic acid), and HCHO (formaldehyde) were determined using passive samplers, as well as temperature and relative humidity data loggers. In addition, two specific short-term monitoring campaigns focused on NH3 were performed to evaluate the influence of visitor presence on indoor concentrations of the above compounds and environmental parameters. NH3 and HCHO concentrations spiked during high visitor occupancy, with NH3 levels doubling in crowded periods. Short-term NH3 campaigns confirmed a direct correlation between visitor numbers and the above indoor concentrations, likely due to human emissions (e.g., sweat, breath) and off-gassing from materials. The indoor/outdoor ratios indicated that several pollutants originated from indoor sources, with ammonia and acetic acid showing the highest indoor concentrations. By measuring the number of visitors and microclimate parameters (temperature and humidity) every 3 s, we were able to precisely estimate the causality and the temporal shift between these quantities, both at small time scale (a few minute delay between peaks) and at medium time scale (daily average conditions due to the continuous inflow and outflow of visitors). Full article
(This article belongs to the Section Air Quality)
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18 pages, 16917 KiB  
Article
Unraveling the Spatiotemporal Dynamics of Rubber Phenology in Hainan Island, China: A Multi-Sensor Remote Sensing and Climate Drivers Analysis
by Hongyan Lai, Bangqian Chen, Guizhen Wang, Xiong Yin, Xincheng Wang, Ting Yun, Guoyu Lan, Zhixiang Wu, Kai Jia and Weili Kou
Remote Sens. 2025, 17(14), 2403; https://doi.org/10.3390/rs17142403 - 11 Jul 2025
Cited by 1 | Viewed by 275
Abstract
Rubber Tree (Hevea brasiliensis) phenology critically influences tropical plantation productivity and carbon cycling, yet topography and climate impacts remain unclear. By integrating multi-sensor remote sensing (2001–2020) and Google Earth Engine, this study analyzed spatiotemporal dynamics in Hainan Island, China. Results reveal [...] Read more.
Rubber Tree (Hevea brasiliensis) phenology critically influences tropical plantation productivity and carbon cycling, yet topography and climate impacts remain unclear. By integrating multi-sensor remote sensing (2001–2020) and Google Earth Engine, this study analyzed spatiotemporal dynamics in Hainan Island, China. Results reveal that both the start (SOS occurred between early and late March: day of year, DOY 60–81) and end (EOS occurred late January to early February: DOY 392–406, counted from the previous year) of the growing season exhibit progressive delays from the southeast to northwest, yielding a 10–11 month growing season length (LOS). Significantly, LOS extended by 4.9 days per decade (p < 0.01), despite no significant trends in SOS advancement (−1.1 days per decade) or EOS delay (+3.7 days per decade). Topographic modulation was evident: the SOS was delayed by 0.27 days per 100 m elevation rise (p < 0.01), while the EOS was delayed by 0.07 days per 1° slope increase (p < 0.01). Climatically, a 100 mm precipitation increase advanced SOS/EOS by approximately 1.0 day (p < 0.05), preseasonally, a 1 °C February temperature rise advanced the SOS and EOS by 0.49 and 0.53 days, respectively, and a 100 mm January precipitation increase accelerated EOS by 2.7 days (p < 0.01). These findings advance our mechanistic understanding of rubber phenological responses to climate and topographic gradients, providing actionable insights for sustainable plantation management and tropical forest ecosystem adaptation under changing climatic conditions. Full article
(This article belongs to the Section Environmental Remote Sensing)
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25 pages, 6820 KiB  
Article
Coccolithophore Assemblage Dynamics and Emiliania huxleyi Morphological Patterns During Three Sampling Campaigns Between 2017 and 2019 in the South Aegean Sea (Greece, NE Mediterranean)
by Patrick James F. Penales, Elisavet Skampa, Margarita D. Dimiza, Constantine Parinos, Dimitris Velaoras, Alexandra Pavlidou, Elisa Malinverno, Alexandra Gogou and Maria V. Triantaphyllou
Geosciences 2025, 15(7), 268; https://doi.org/10.3390/geosciences15070268 - 11 Jul 2025
Viewed by 651
Abstract
This study presents the living coccolithophore communities and the morphological variability of Emiliania huxleyi in the South Aegean Sea from three sampling regions during winter-early spring (March 2017, March 2019) and summer (August 2019). Emphasis is given to March 2017 to monitor the [...] Read more.
This study presents the living coccolithophore communities and the morphological variability of Emiliania huxleyi in the South Aegean Sea from three sampling regions during winter-early spring (March 2017, March 2019) and summer (August 2019). Emphasis is given to March 2017 to monitor the variations in coccolithophore assemblages after an exceptionally cold event in December 2016, which resulted in newly produced dense waters that ventilated the Aegean deep basins. The assemblages displayed distinct seasonality with the predominance of E. huxleyi and Syracosphaera molischii during winter-early spring, associated with the water column mixing. By contrast, summer assemblages were featured by holococcolithophores and typical taxa of warm, oligotrophic upper waters. It seems that the phytoplanktonic succession as well as the nutrient supply to the upper euphotic layers were affected by the water column perturbation during the extreme winter of 2016–2017, which led to strong convective mixing and dense water formation. The decreased coccosphere densities during March 2017, accompanied by the notable presence of diatoms, were most probably associated with a prolonged diatom bloom, causing delay in the development of the coccolithophore community and resulting in a nitrogen-limited setting. Emiliania huxleyi morphometry showed the characteristic seasonal calcification trend of the Aegean, with the dominance of smaller coccoliths in the summer and increased coccolith length and width during the cold season. The intense cold conditions and wind-induced mixing during the winter of 2016–2017 possibly increased the absorption of atmospheric CO2 in surface waters, causing increased acidity and the subsequent presence of etched/undercalcified E. huxleyi coccoliths and other taxa, most probably implying in situ calcite dissolution. Full article
(This article belongs to the Section Biogeosciences)
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18 pages, 269 KiB  
Article
Effect of Plant Topping on Seasonal Development, Physiological Changes, and Grain Yield of Soybean
by Sora Lee, Chaelin Jo, Miri Choi, Jihyeon Lee, Nayoung Choi and Chaein Na
Plants 2025, 14(13), 2068; https://doi.org/10.3390/plants14132068 - 6 Jul 2025
Viewed by 470
Abstract
Soybean (Glycine max L.) is vulnerable to environmental stresses, such as heavy rainfall and high winds, which promote lodging and reduce plant performance during the monsoon season. To mitigate these issues, we evaluated the effects of plant topping, a practice involving the [...] Read more.
Soybean (Glycine max L.) is vulnerable to environmental stresses, such as heavy rainfall and high winds, which promote lodging and reduce plant performance during the monsoon season. To mitigate these issues, we evaluated the effects of plant topping, a practice involving the removal of apical buds, on plant architecture, physiological traits, and grain yield in four soybean cultivars over two growing seasons (2021–2022). Plant topping was performed at the V6-7 stage by cutting 30–35 cm above the ground. Plant topping reduced plant height by up to 23.5% and decreased leaf area index (by 8.0–16.4%), potentially improving light penetration into the lower canopy. Although chlorophyll concentration declined temporarily (297.8 vs. 272.8 mg m−2 for non-topping vs. topping, respectively), NDVI remained stable, indicating delayed senescence. Chlorophyll fluorescence parameters revealed cultivar-specific stress responses, particularly in Taegwang, which showed elevated ABS/RC, TR0/RC, and DI0/CS values under plant topping. Grain yield was generally unaffected, except in Jinpung, which increased by 34% under plant topping in 2021 (2701 kg ha−1 vs. 3621 kg ha−1 for non-topping vs. topping). In conclusion, plant topping may help improve canopy structure and light distribution without compromising yield, potentially reducing lodging risk and offering a cultivar-specific management strategy. Full article
23 pages, 3984 KiB  
Article
Stem Heating Enhances Growth but Reduces Earlywood Lumen Size in Two Pine Species and a Ring-Porous Oak
by J. Julio Camarero, Filipe Campelo, Jesús Revilla de Lucas, Michele Colangelo and Álvaro Rubio-Cuadrado
Forests 2025, 16(7), 1080; https://doi.org/10.3390/f16071080 - 28 Jun 2025
Viewed by 296
Abstract
Climate models forecast warmer winter conditions, which could lead to an earlier spring xylem phenology in trees. Localized stem heat experiments mimic this situation and have shown that stem warming leads to an earlier cambial resumption in evergreen conifers. However, there are still [...] Read more.
Climate models forecast warmer winter conditions, which could lead to an earlier spring xylem phenology in trees. Localized stem heat experiments mimic this situation and have shown that stem warming leads to an earlier cambial resumption in evergreen conifers. However, there are still few comprehensive studies comparing the responses to stem heating in coexisting conifers and hardwoods, particularly in drought-prone regions where temperatures are rising. We addressed this issue by comparing the responses (xylem phenology, wood anatomy, growth, and sapwood concentrations of non-structural carbohydrates—NSCs) of two pines (the Eurosiberian Pinus sylvestris L., and the Mediterranean Pinus pinaster Ait.) and a ring-porous oak (Quercus pyrenaica Willd.) to stem heating. We used the Vaganov-Shashkin growth model (VS model) to simulate growth phenology considering several emission scenarios and warming rates. Stem heating in winter advanced cambial phenology in P. pinaster and Q. pyrenaica and enhanced radial growth of the three species 1–2 years after the treatment, but reduced the transversal lumen area of earlywood conduits. P. sylvestris showed a rapid and high growth enhancement, whereas the oak responded with a 1-year delay. Heated P. pinaster and Q. pyrenaica trees showed lower sapwood starch concentrations than non-heated trees. These results partially agree with projections of the VS model, which forecasts earlier growth onset, particularly in P. pinaster, as climate warms. Climate-growth correlations show that growth may be enhanced by warm conditions in late winter but also reduced if this is followed by dry-warm growing seasons. Therefore, forecasted advancements of xylem onset in spring in response to warmer winters may not necessarily translate into enhanced growth if warming reduces the hydraulic conductivity and growing seasons become drier. Full article
(This article belongs to the Special Issue Drought Tolerance in ​Trees: Growth and Physiology)
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43 pages, 15788 KiB  
Article
Mechanisms Driving the Nonlinear Relationship Between Soil Freeze–Thaw Cycles and NDVI from Remotely Sensed Data in the Eastern Tibetan Plateau
by Yixuan Wang, Quanzhi Yuan and Ping Ren
Remote Sens. 2025, 17(13), 2192; https://doi.org/10.3390/rs17132192 - 25 Jun 2025
Viewed by 357
Abstract
Climate warming leads to earlier onset and shortened duration of the freeze–thaw period in the eastern Tibetan Plateau, which has complex effects on vegetation growth. We assessed the spatiotemporal changes in the freeze–thaw period, evaluated its relationship with Normalized Difference Vegetation Index (NDVI [...] Read more.
Climate warming leads to earlier onset and shortened duration of the freeze–thaw period in the eastern Tibetan Plateau, which has complex effects on vegetation growth. We assessed the spatiotemporal changes in the freeze–thaw period, evaluated its relationship with Normalized Difference Vegetation Index (NDVI from remotely sensed data), used the Panel Smooth Threshold Regression (PSTR) model to quantify the nonlinear impacts and identify critical thresholds, and applied ridge regression to explore the dominant mechanisms under different climatic conditions. The results showed the following: (1) The duration of the freeze–thaw transition period showed strong latitudinal zonality, with stronger spring disturbances than autumn ones. The trend of soil freeze–thaw status in high-altitude areas is the most significant, with a significant increase in the complete thaw period (CTP) and a significant decrease in the complete freeze period (CFP). (2) The earlier onset of the spring freeze–thaw period (SFTTP) and the CTP benefits vegetation growth in both early and late seasons. The delayed autumn freeze–thaw period (AFTTP) benefits early-season vegetation growth but is less favorable for late-season growth. The delayed CFP is beneficial for vegetation growth throughout the year. (3) The CTP’s boost to NDVI collapses at an onset date of 110 days and duration of 190 days. The AFTTP’s benefit peaks at an onset date of 300 days. (4) Temperature and the CTP are key drivers of NDVI changes, especially in the mid-to-late growing season. Arid areas respond strongly to freeze–thaw disturbances, while moderate precipitation areas are less affected. This study is the first to quantitatively analyze the nonlinear mechanism of the freeze–thaw–vegetation relationship, offering a new theoretical basis. Full article
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19 pages, 4035 KiB  
Article
Impact of Short-Term and Prolonged (Multi-Year) Droughts on Tree Mortality at the Individual Tree and Stand Levels
by Goran Češljar, Zvonimir Baković, Ilija Đorđević, Saša Eremija, Aleksandar Lučić, Ivana Živanović and Bojan Konatar
Plants 2025, 14(13), 1904; https://doi.org/10.3390/plants14131904 - 20 Jun 2025
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Abstract
Droughts accompanied by high temperatures are becoming increasingly frequent across Europe and globally. Both individual trees and entire forest ecosystems are exposed to drought stress, with prolonged drought periods leading to increased tree mortality. Therefore, continuous monitoring, data collection, and analysis of tree [...] Read more.
Droughts accompanied by high temperatures are becoming increasingly frequent across Europe and globally. Both individual trees and entire forest ecosystems are exposed to drought stress, with prolonged drought periods leading to increased tree mortality. Therefore, continuous monitoring, data collection, and analysis of tree mortality are essential prerequisites for understanding the complex interactions between climate and trees. This study examined the effects of short-term and prolonged (multi-year) droughts on the mortality of individual trees and forests in Serbia. The analysis was based on datasets from our previous research on the influence of drought and drought duration on individual tree mortality in Serbian forest ecosystems, supplemented with new data collected through the International Co-operative Programme on Assessment and Monitoring of Air Pollution Effects on Forests (ICP Forests). Additionally, we incorporated data from the public enterprise (PE) “Srbijašume”, which manages forests in Central Serbia, focusing on random yields resulting from natural disasters (droughts). These data enabled a comparative assessment of the findings on increased mortality and drought impact at both the individual tree level and the stand level. This study identifies key similarities and differences in tree mortality trends based on drought duration and examines their correlations within the same time frame (2004–2023). By analysing climatic conditions across Serbia, we provide evidence of the interaction between drought periods and increased forest mortality, which we further confirmed by calculating the Standardized Precipitation Evapotranspiration Index (SPEI). We also address the tree species that were most sensitive to the effects of drought. Our findings indicate that prolonged (multi-year) droughts, accompanied by high temperatures, have significantly contributed to increased tree mortality over the past decade. Successive multi-year droughts pose a substantial threat to both individual trees and entire forests, producing more severe and persistent responses compared to those caused by single-year droughts, which forests and individual trees are generally more capable of tolerating. Moreover, due to prolonged drought stress, trees weaken, leading to delayed mortality that may manifest several years after the initial drought event. The observed increase in tree mortality has been found to correlate with rising temperatures and the growing frequency of prolonged droughts over the past decade. Especially, intense droughts in the growing season (April–September) have a very negative impact on forests. Full article
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