Search Results (72)

As the main byproduct of edible mushroom processing, mushroom stems are often discarded due to their rough texture and poor palatability, resulting in resource waste. However, as a ruminant animal, sika deer has good fiber digestion ability, providing the possibility for the resource utilization of mushroom stems. This study evaluated the effects of dietary supplementation with Lentinus edodes stem powder (LESP) on growth performance, nutrient digestibility, serum biochemical parameters, immune responses, and gut microbiota composition in sika deer fawns. A total of 120 weaned fawns (120 ± 10 days old; 35.0 ± 1.0 kg body weight) were randomly assigned to four dietary treatments, receiving a basal diet supplemented with 0% (control), 2.5% (LE1), 5% (LE2), or 7.5% (LE3) LESP for 35 days. Growth performance did not differ significantly among treatments (p > 0.05). However, apparent digestibility of ether extract and crude protein was significantly higher in the LE1 group (p < 0.05), while LE3 exhibited reduced digestibility of organic matter, ether extract, and crude protein (p < 0.05). Serum total protein (TP) and albumin (ALB) levels increased with LESP inclusion, with the highest values observed in LE3 (p < 0.05). Serum glucose (GLU) decreased dose-dependently (p < 0.05). Immunoglobulin A (IgA), G (IgG), and M (IgM) concentrations were significantly elevated in LE2 and LE3 groups (p < 0.05). Gut microbiota analysis revealed that Firmicutes, Bacteroidetes, and Actinobacteria were the dominant phyla. LE3 supplementation altered beta diversity and reduced microbial evenness. At the genus level, LE2 and LE3 increased the abundance of beneficial taxa such as Planomicrobium and Psychrobacter, while decreasing Ruminococcus and Clostridiaceae_Clostridium. In conclusion, adding 5% LESP to the diet can increase the digestibility of nutrients, enhance humoral immunity, and regulate the composition of the gut microbiota. This study promotes the transformation of mushroom stems from agricultural waste into functional feed additives, which helps reduce breeding costs, improve animal health, and provide a practical path for the coordinated and sustainable development of the edible mushroom industry and animal husbandry. Full article

Heat stress is a growing concern in cattle production systems due to the increasing frequency and intensity of extreme weather events driven by climate change. This review synthesizes current knowledge on the multifaceted impacts of heat stress and focuses on nutritional strategies to mitigate its effects on ruminating cattle. A comprehensive literature search was conducted using PubMed, Scopus, Web of Science, and Google Scholar. Heat stress adversely affects cattle physiology, behavior, rumen function, and overall productivity, particularly in dairy animals with high metabolic activity. During heat stress episodes, changes in the microbial population have been reported; however, there is no clear consensus, as findings vary widely among studies depending on diet, feed intake, animal type and experimental design. This variability limits the ability to draw general conclusions regarding changes in the rumen microbiome driven by heat stress. In this context, dietary nutritional intervention strategies offer a practical and scalable approach to enhance thermotolerance and maintain performance under heat stress conditions. Key nutritional strategies include modifications in diet composition to reduce metabolic heat production, with some approaches carrying potential risks to animal health, e.g., increasing dietary energy density through concentrates while minimizing forage content. Supplementation with rumen-protected nutrients like amino acids, vitamins, and minerals can be used to support immune function, antioxidant capacity, and metabolic stability. Polyphenols and betaine contribute to oxidative stress reduction and gut integrity, while probiotics may be used to improve rumen fermentation and nutrient utilization. Sensor technologies, including rumen boluses and wearable devices, offer the potential to monitor physiological responses to heat stress in real time and offer opportunities for precision feeding and early intervention. Most published studies only cover short periods of heat stress, and there is a lack of in vitro models simulating rumen hyperthermia. In parallel, future research should therefore prioritize longitudinal, in vivo trials that integrate physiological, metabolic, and microbial responses to understand the long term and systemic effect of heat stress. In addition, controlled trials in commercial settings are necessary to prove the transferability of results to commercial herds. A multidisciplinary approach combining nutritional, environmental, and technological strategies is likely to play an important role in safeguarding cattle welfare and productivity in a warming climate. Full article

Repeat breeding syndrome (RBS), characterized by repeated artificial insemination failure, is associated with ovarian dysfunction in ruminants and causes economic losses. Environmental endocrine-disrupting chemicals (EDCs), such as bisphenol F (BPF), may contribute to RBS, but the relationship between BPF exposure and RBS in cows remains unclear. In this study, we conducted an epidemiological investigation and clinical examinations in 111 adult Simmental cows, including 7 cows diagnosed with RBS, from two local farms, and measured the BPF levels in the drinking water, feed, and the serum samples. We further evaluated the protective effects of phillyrin, a bioactive compound derived from Forsythia suspensa, against BPF-induced ovarian toxicity using cow granulosa cells (CGCs). RBS mainly occurred in young cows with two or more parities. RBS cows showed signs of potential liver dysfunction, kidney injury, anemia, and fecal abnormalities suggestive of altered gut health, together with higher serum BPF levels and lower estradiol levels. Detectable BPF contamination in the drinking water and feed suggested environmental exposure as a possible contributor to RBS in cows. In vitro, BPF induced inflammatory and apoptotic responses in CGCs by upregulating pro-inflammatory and pro-apoptotic mediators, whereas phillyrin pretreatment markedly alleviated these effects. These findings suggest that BPF exposure might be associated with RBS in cows and phillyrin could be used as a promising natural agent for mitigating BPF-related reproductive toxicity. Full article

Alterations in microbiota, transcript and metabolites are critical to intestinal homeostasis and host health. This study used a combination of 16s rRNA, transcriptome sequencing and liquid chromatography–mass spectrometry to investigate intestinal microbiota, genes and metabolic profiles in the ileum of Hu sheep fed broccoli extract. Here, we randomly allocated 14 Hu sheep to two diets: a basal diet without any supplementation (NC) and a basal diet supplemented with 200 mg/kg broccoli tail (BT). After 60 days of treatment, blood and jejunal samples were collected for serum biochemical indicators and multi-omics analysis. In this study, the extract of broccoli tails had a significant effect on the serum biochemical indicators, including white blood cells, red blood cells, mean corpuscular volume, mean corpuscular hemoglobin concentration, mean platelet volume, triglycerides and total protein in Hu sheep (p < 0.05). Transcriptomic analysis showed that the 672 differentially expressed genes between the NC and BT groups were primarily enriched in linoleic acid metabolism, steroid hormone biosynthesis, and cholesterol metabolism. Metabolomics analysis using Kyoto Encyclopedia of Genes and Genomes enrichment showed that the 41 differentially abundant metabolites were mainly enriched in bile secretion, vitamin B6 metabolism, and the mTOR signaling pathway. 16S rRNA sequencing results indicated that the extract of broccoli tails increased the relative abundance of Peptostreptococcaceae and decreased the relative abundance of Lachnospiraceae, Lachnospirales, and Bacteroidaceae. Integrated transcriptome, metabolome, and microbiome analysis showed that the gut microbiota and host transcriptomic changes may participate in systemic metabolic regulation by modulating amino acid metabolism, lipid signal transduction, nucleotide metabolism, and vitamin B6-related metabolic pathways. These findings demonstrate that the extract of broccoli tails modulates intestinal gene expression, systemic metabolism, and gut microbial ecology in Hu sheep, providing new insights into the utilization of agricultural byproducts as a functional feed supplement for ruminants. Full article

Folate is a central nutrient in one-carbon metabolism, contributing to nucleotide biosynthesis, methionine cycling, methyl-donor supply, and epigenetic regulation. In animals, the intestine is both a principal site of folate absorption and a key target organ for folate action. This narrative review focuses primarily on livestock, poultry, aquaculture species, ruminants, and animal-source food enrichment, while also using rodent, human, and in vitro studies as mechanistic or translational evidence. We synthesize evidence on folate absorption, transport, and metabolism and evaluate the mechanisms through which folate influences intestinal health. Available evidence suggests that adequate folate supply may support epithelial renewal, tight-junction integrity, mucosal immune balance, antioxidant capacity, gut microbiota stability, short-chain fatty acid production, and epigenetic regulation of intestinal development. These effects have been reported in poultry, pigs, fish, ruminants, rodents, and maternal–offspring models. However, the evidence is uneven across species, and dose–response relationships, folate forms, bioavailability, and species-specific requirements remain major limitations for translating current knowledge into animal production. Future studies should compare folic acid, 5-methyltetrahydrofolate, natural reduced folates, microbiota-derived folate, and folate-producing probiotics; quantify the contribution of microbiota-derived folate to host methyl-donor pools; and develop precision strategies that integrate folate with other one-carbon nutrients, probiotics, and product-enrichment technologies. Full article

Modern dairy production has greatly increased milk yield, but high productivity is often accompanied by greater metabolic pressure, particularly during the transition period. Ketosis, fatty liver, and subacute ruminal acidosis are major disorders that limit health, efficiency, and sustainability in high-yielding dairy ruminants. This review examines the rumen microbiota as a central biological interface linking diet, ruminal fermentation, epithelial function, hepatic metabolism, and inflammation. Under homeostatic conditions, the rumen microbiota supports lactation by converting dietary fibre, starch, and nitrogen into volatile fatty acids, microbial protein, and other metabolites required for gluconeogenesis, milk component synthesis, and epithelial maintenance. However, under excessive nutritional or physiological stress, especially high-concentrate feeding and periparturient negative energy balance, this system may shift toward dysbiosis, acid accumulation, lipopolysaccharide release, epithelial barrier impairment, and activation of gut–liver inflammatory pathways. These changes can contribute to the occurrence and interaction of subacute ruminal acidosis, ketosis, and fatty liver. We further summarize key factors affecting rumen microbial stability, including diet structure, host variation, physiological stage, environmental stress, feeding management, and ruminal epithelial volatile fatty acid absorption. Finally, microbiome-oriented strategies, such as gradual dietary transition, nutritional preconditioning, probiotics, postbiotics, functional metabolites, host metabolic support, and epithelial-targeted interventions, are discussed. Maintaining rumen microbial homeostasis should be regarded as a core principle for balancing high milk yield with long-term metabolic health. Future research should move beyond descriptive profiling toward causal validation of host–microbe interactions and the development of microbiome-based early-warning and individualized nutritional management systems. Full article

Seasonal environmental variations significantly affect the health and survival of wild ruminants such as sika deer (C. n. kopschi). This study characterized the adaptation of gut microbial communities to these seasonal shifts, supporting the host’s physiological needs. We employed high-throughput sequencing on sixty fecal samples collected throughout the four seasons to assess microbial diversity, composition, and potential functions. Our findings revealed significant seasonal fluctuations, with microbial diversity peaking in summer and reaching its nadir in winter. Taxonomic analysis showed that Firmicutes was the dominant phylum during spring (63.43%), summer (78.52%), and autumn (81.99%), whereas winter was dominated by Proteobacteria (50.60%), primarily due to the enrichment of Acinetobacter and Pseudomonas. Although the community structure formed three distinct seasonal enterotypes with dramatic shifts in bacterial taxa, functional profiling based on genomic databases indicated that core biological pathways remained remarkably stable throughout the year. These results demonstrate that sika deer maintain internal functional homeostasis by reorganizing their gut microbial composition to address harsh environmental stressors. This research provides a critical scientific basis for understanding the adaptive resilience of wild animals, offering valuable insights into the conservation and management of wildlife populations in dynamic ecosystems. Full article

Growing Angus steers (n = 20) were blocked by weight and randomly assigned to one of two treatment groups: Control group (CON, n = 10) fed a feedlot ration ad libitum, or a ruminally protected hydrogenated fat-embedded calcium gluconate (HFCG) treatment group (HFCG, n = 10), which was fed the control ration top-dressed at 16 g/head/day for 55 days. During the slaughter process, digesta samples were collected from the cecum, colon, and rectum. Acetate concentrations were greater in the cecal and rectal digesta of steers (p ≤ 0.05) in the HFCG treatment group. Propionate concentrations were greater in the cecal, colonic, and rectal (p ≤ 0.05) digesta of steers in the HFCG treatment group. Butyrate concentrations were greater (p = 0.098) in the colon digesta of steers in the HFCG treatment group; however, they were not different in the cecal and rectal digesta. To determine the microbial composition within each section of the hindgut, DNA was extracted, and 16S rRNA gene sequencing was performed. Data were analyzed using a General Linear Model with dietary treatment as the main effect. Species richness in the cecum, colon, and rectum was not different between treatments. Erysipelotrichaceae, Peptostreptococcaceae, and Atopobiaceae abundances were increased (p ≤ 0.05) in the cecal bacterial community of steers in the HFCG group, while a significant decrease (p ≤ 0.05) in Rikenellaceae and Muribaculaceae abundances was recorded within the same bacterial community. In the colon bacterial community of steers in the HFCG group, Ruminococcaceae and Muribaculaceae abundances were elevated (p ≤ 0.05), while there was a reduction (p ≤ 0.05) in Lachnospiraceae, Erysipelotrichaceae, Atopobiaceae, and Peptostreptococcaceae abundances. Paeniclostridium, Romboutsia, and Turicibacter abundances were increased (p ≤ 0.05) in the cecal bacterial community of steers in the HFCG group, while there was a decrease (p ≤ 0.05) in Rikenellaceae_RC9 _gut_group abundance within the same bacterial community. In the colon microbiota of steers in the HFCG group, Turicibacter abundance was decreased (p ≤ 0.05). Supplementing growing steers with HFCG impacted some members of the bacterial populations, which have important roles in gut homeostasis and health, along with the formation of beneficial end-products in the gastrointestinal tract. Full article

Enteric diseases are a leading cause of morbidity and economic loss in livestock production, and the search for effective, antibiotic-free alternatives has intensified in recent years. Among emerging strategies, yeast-derived postbiotics—non-viable microbial cells and their metabolites—have gained attention for their potential to enhance gut health and disease resistance in farm animals. This review synthesizes current knowledge on the composition, mechanisms of action, and practical applications of yeast postbiotics, particularly those derived from Saccharomyces cerevisiae and related species. Key bioactive components, such as β-glucans, mannan oligosaccharides, peptides, and organic acids, are discussed in the context of their immunomodulatory, anti-inflammatory, and pathogen-inhibitory properties. Evidence from in vivo and in vitro studies across multiple livestock species—including poultry, swine, and ruminants—demonstrates beneficial effects on intestinal barrier function, microbial balance, and performance under disease-challenged conditions. Despite promising outcomes, challenges remain in standardizing postbiotic preparations, elucidating dose–response relationships, and tailoring applications to species-specific needs and production systems. This review highlights the potential of yeast postbiotics as a sustainable tool in enteric disease management and outlines research priorities for their broader implementation in animal agriculture. Full article

The use of antibiotics as growth promoters in livestock production has contributed to the emergence and spread of antimicrobial resistance (AMR), posing a significant global public health threat specifically from the projected mortality burden. Although many countries have restricted the non-therapeutic use of antibiotics, practical and effective alternatives are still required to maintain livestock productivity. This scoping review examines the current evidence on non-antibiotic compounds evaluated as growth-promoting agents in livestock production. The primary objective of this search was to generate a comprehensive list of commonly applied alternatives to antibiotics used as growth promoters in livestock systems. A search was conducted in the CAB Abstracts, Web of Science Core Collection, and AGRICOLA databases. Prior to the scoping review, an initial list of alternatives to antibiotic components was generated through a screening of selected scientific sources and subsequently verified using Google Scholar for the period 2010–2025. This list included brief descriptions of each component, which were used to inform the keyword strategy for the scoping review. Eligible studies were screened in accordance with PRISMA-ScR guidelines, and data were extracted on compound type, livestock species, geographic region, and reported performance outcomes. The alternatives identified included probiotics and prebiotics, phytogenic compounds and essential oils, enzymes and organic acids, vaccines and immunostimulants, bacteriophages, and competitive exclusion products. A total of 1230 records were retrieved and imported into Zotero for reference management. After removal of duplicate records using Zotero’s built-in deduplication tool, 377 unique records remained for screening. Overall, these compounds demonstrated variable effects on feed efficiency, weight gain, and gut health. However, most studies were limited in scale, duration, and methodological consistency. As a result, comprehensive comparative trials and large-scale field evaluations are needed to support evidence-based policy recommendations and the sustainable implementation of alternatives to antibiotics in livestock production systems. Our findings identified six major categories that represent the most frequently reported alternatives to antibiotic growth promoters. Although probiotics, phytogenic, and organic acids were the most extensively studied, substantial heterogeneity in trial design, dosage, and production systems limited meaningful cross-comparisons. In addition, most studies focused on poultry and swine, with comparatively fewer investigations involving ruminant species. This scoping review was not intended to evaluate the efficacy or practical applicability of these alternatives; such assessments require further standardized and extensive studies before recommendations for their widespread application can be made. Full article

Dietary fiber (DF) is a fundamental component of animal nutrition and has been widely studied for its nutritional and physiological functions in animals. While existing studies mainly focus on the independent effects of DF on gut microbiota or bile acids (BAs), the mechanisms underlying their interactions remain poorly understood. DF interacts closely with gut microbiota, promoting the production of beneficial metabolites such as short-chain fatty acids, which subsequently influence BA metabolism through microbial deconjugation and dehydroxylation processes, generating free and secondary BA essential for host health. Together, the gut microbiota and BA play key roles in mediating the effects of DF on intestinal and systemic physiology via the gut–liver axis. Although DF contributes to energy supply, nutrient digestion, and regulation of gut microbiota and BA metabolism, its physiological effects vary depending on fiber source, type, chemical composition, inclusion level, and animal species. Ruminant and non-ruminant animals differ in their capacity to utilize DF, with extensive fermentation occurring in the rumen of ruminants, whereas fermentation in non-ruminants mainly occurs in the hindgut and is more limited. Consequently, inappropriate DF supplementation may impair gastrointestinal function and overall physiological status. This review summarizes the diverse effects of different DF types in animals and critically examines the complex and bidirectional interactions among DF, gut microbiota, and BA metabolism, highlighting knowledge gaps that require further investigation to optimize DF application in animal nutrition. Full article

Microecological preparations exert beneficial effects on the health of young ruminant animals; however, the mechanism is unclear. As a result, the present study analyzed the effects of yeast cultures on the growth properties, microbiome, and metabolism of weaned lambs. In this study, a total of 20 weaned lambs were randomly, stochastically divided into four teams: the control group (Group A) were fed a basic diet; Group B were fed with Saccharomyces cerevisiae BC strain culture (30 g/head/d); Group C were fed with Kluyveromyces marquez XR4 strain culture (30 g/head/d); Group D were fed with a composite culture of the two yeast strains (30 g/head/d). The study lasted for 40 days, with daily records of lamb feed intake and weight. Lamb feces were collected regularly for metagenomic sequencing and metabolomics analysis. The average daily weight gain and average daily yield of Group D lambs were significantly higher than those of Group A lambs (p < 0.01). The feed utilization rate in the yeast-fed groups was considerably higher than in the control group (p < 0.05), indicating that the addition of yeast crops to lamb feed might improve lamb feed performance. Bacteroides and the mTOR signaling pathway were dramatically enriched in the intestines of weaned lambs in the yeast-culture-fed groups, and their expression levels of ketones and benzoic acid compounds were significantly upregulated. These results indicated that yeast culture had excellent effects on weaned lambs in regulating immunological functioning and the intestinal environment, protecting the enteric mucosal barrier, improving digestion and nutritional absorption, and enhancing antioxidant function. In summary, adding yeast culture to weaned lamb feed can generate a positive effect on its productivity performance and gut health. These findings provide novel insights into promoting the health of young ruminants. Full article

Diarrhea in kids is a significant health and economic concern for small-scale ruminant farms. This study aims to investigate the properties of Lactobacillus amylovorus as a treatment for kids with diarrhea and its effect on the composition of the gut microbiota. A total of 20 neonatal goats (approximately 2 months old) were divided into three groups: healthy control (HC, n = 4), diarrhea (D, n = 8), and diarrhea treated with probiotic (DT, n = 8). We tracked gut microbial profiles, fecal consistency, short-chain fatty acids (SCFAs), and clinical symptoms. Probiotic-treated kids recovered fully from diarrhea within two weeks, while their untreated counterparts showed signs of clinical deterioration and gradual emaciation. Kids with diarrhea had lower microbial richness, according to alpha diversity analysis, and this was only partially restored after probiotic treatment. The kids with diarrhea had the lowest Shannon, ACE, Simpson, Dominance, Pielou-e, and Chao1 indices compared to the HC group, while the administration of Lactobacillus amylovorus significantly (p < 0.05) restored their normal enrichment in the DT group compared to the D group. The healthy group had a higher abundance of Verrucomicrobiota, while Firmicutes and Bacteroidota predominated in all groups. Bacteroides and Akkermansia predominated in the healthy and treated groups. At the genus level, analysis showed elevated levels of Escherichia-Shigella and UCG-005 in kids with diarrhea. In addition, the concentration of each SCFA in the D group was significantly (p < 0.05) lower than in the HC group. This study provides novel evidence that Lactobacillus amylovorus administration not only alleviates diarrhea but also uniquely restores the production of key SCFAs—including butyrate, acetate, and propionate—in neonatal goats, a finding not previously reported in this species. The concurrent recovery of microbial diversity and SCFA profiles highlights the dual mechanistic potential of Lactobacillus amylovorus as a gut microbiota modulator and metabolic therapeutic in young ruminants. These results lend credence to its potential as a probiotic treatment for small ruminant enteric diseases. Full article

Growing limitations on the use of in-feed antibiotics have accelerated the search for functional feed additives capable of supporting animal health and productivity under antibiotic-free production systems. Postbiotics, defined as non-viable microbial products or metabolic byproducts, and phytogenics, which are plant-derived bioactive compounds, have emerged as promising alternatives due to their stability and biological activity. Recent advances in the application of postbiotics and phytogenics in monogastric and ruminant nutrition are summarized, with emphasis on their mechanisms of action, synergistic effects, and impacts on gut health, immune function, and growth performance. Postbiotics modulate the gut microbiota, enhance epithelial barrier integrity, and regulate immune signaling, whereas phytogenic compounds provide antimicrobial, antioxidant, and digestive-stimulant effects. Available evidence suggests that combined strategies can enhance efficacy, particularly under production-related stress. Key challenges related to formulation, dose–response relationships, stability, and regulatory classification are discussed together with emerging omics-based approaches that support precision formulation. Overall, integration of multi-omics evidence with formulation and regulatory considerations supports the practical use of postbiotics and phytogenics in commercial livestock systems. Full article

Early-life development of immune functions is crucial for calf health, growth, and future productivity. Galacto-oligosaccharides (GOSs) have been reported to facilitate ruminal microbial establishment and improve growth in Holstein dairy calves, but their prolonged influence on immunoglobulin levels, hindgut microbiota, and metabolic regulation remains insufficiently understood. This study evaluated the effects of early-life GOS supplementation on immune-related indicators, intestinal microbial ecology, and metabolic profiles in Holstein calves. Twenty-four newborn Holstein female dairy calves were randomly assigned to a control group (CON, n = 12) or a GOS group (GOS, n = 12; 10 g/day from birth to day 28). After supplementation ceased on day 28, calves previously receiving GOS were referred to as the GOSS group (n = 6). Immunoglobulin levels, gut microbiota, and fecal and serum metabolomes were evaluated during supplementation and six weeks after withdrawal. GOS supplementation significantly increased serum IgA and IgG levels during the treatment, with IgG levels remaining elevated for six weeks after discontinued supplementation. Although overall microbial diversity was not markedly altered, GOS selectively enriched bacterial taxa and function pathways linked to amino acid synthesis, unsaturated fatty acid production, and coenzyme-related metabolism. On day 70, GOSS group displayed distinct fecal and serum metabolomic profiles, with altered metabolites primarily associated with vitamin B6, folate, cobalamin metabolism, branched-chain amino acid biosynthesis, and purine and arginine pathways. These results demonstrate that early-life GOS supplementation promotes sustained immune and metabolic alterations following supplementation cessation, potentially mediated by modulation of gut microbial functions. These findings suggest that early dietary GOS supplementation may support physiological maturation in calves and could be useful as a nutritional strategy in calf-rearing systems. Full article