Search Results (12,132)

(1) Background: Ultra-early optical coherence tomography (OCT) changes following intravitreal injection may reflect transient mechanical compression rather than pharmacologic effects; however, this temporal profile has not been rigorously characterised with appropriate statistical methodology. (2) Methods: In this prospective observational study, 40 eyes of 40 consecutive patients (one per patient) with macular edema secondary to neovascular age-related macular degeneration (nAMD), diabetic macular edema (DME), or chronic central serous retinopathy (CSR) underwent intravitreal bevacizumab (n = 35) or triamcinolone acetonide (n = 5). Goldmann applanation tonometry and spectral-domain OCT were performed at baseline, 2–5 min, 15 ± 5 min, 24 h, and 48 h post-injection. Repeated-measures ANOVA with Greenhouse–Geisser correction, linear regression, and Spearman rank correlation were applied. (3) Results: Central subfield thickness (CST) decreased markedly at 15 ± 5 min (mean −24.8 ± 11.5%; 95% CI: −28.5% to −21.1%; p < 0.001; partial η² = 0.70), with near-complete rebound by 48 h (−1.0%; p = 0.400). Peak intraocular pressure (IOP) elevation correlated with CST reduction (Spearman r_s = 0.61; 95% CI: 0.39–0.77; p < 0.001), and baseline CST predicted thinning magnitude (R² = 0.52; p < 0.001). (4) Conclusions: Ultra-early OCT thinning after intravitreal injection is consistent with transient mechanical compression. Retinal thickness measurements within 48 h post-injection should be interpreted with caution when assessing treatment response, as early anatomic reduction may not reflect pharmacologic efficacy. Full article

Rice starch (RS) is widely consumed, but is usually rapidly digested, which may increase postprandial blood glucose levels. Therefore, regulating RS digestibility is important for development functional starch-based foods. In this study, sodium alginate (NaAlg) was incorporated into RS gels and subsequently crosslinked with Ca²⁺ to form a calcium alginate (CaAlg) network, and its effects on the physicochemical properties, digestion behavior, and physiological responses of RS gels were evaluated. Rheological measurement showed that the Ca²⁺-crosslinked alginate network increased the viscosity and viscoelastic moduli of RS gels. Low-field nuclear magnetic resonance analysis showed that the Ca²⁺-crosslinked alginate network reduced free water mobility. Structural characterization using Fourier-transform infrared spectroscopy, X-ray diffraction, and cold-field scanning electron microscopy shows that the Ca²⁺-crosslinked alginate network was associated with enhanced intermolecular interactions and a more continuous gel network, while all gelatinized samples exhibited predominantly amorphous structures. In vitro digestion experiments showed that the hydrolysis degree at 120 min decreased from 92.3% in RS to 85.6% in HCaAlg/RS. The rapidly digestible starch content significantly decreased from 72.4% to 68.4% (p < 0.05), while resistant starch significantly increased from 7.7% to 14.4% (p < 0.05). First-order kinetic fitting showed that C_∞ significantly decreased from 93.0% to 86.0%, and k significantly decreased from 0.027 to 0.013 min⁻¹ (p < 0.05). In vivo experiments showed that the Ca²⁺-crosslinked alginate/RS gels were associated with a lower postprandial glycemic response, with the incremental area under the curve significantly decreased from 747.2 to 591.7 mmol·min/L (p < 0.05), and the intestinal propulsion rate decreased from 89.6% to 75.3% (p < 0.05). These results suggest that Ca²⁺-crosslinked alginate network formation may modulate the structural properties, digestion behavior, and digestion-related physiological responses of RS gels, providing a basis for the development of starch-based functional foods with improved glycemic control. Full article

This paper analyzes the role of cereal products in the diet of individuals with disorders of carbohydrate metabolism, with particular emphasis on their impact on postprandial glycemia and the risk of developing type 2 diabetes (T2D). Cereal products, as the main source of dietary carbohydrates, also provide dietary fiber, minerals, B vitamins, and key bioactive compounds such as β-glucans, arabinoxylans, resistant starch (RS), and polyphenols. These components may reduce the rate of starch digestion and glucose absorption in the small intestine by increasing the viscosity of intestinal contents or by directly inhibiting digestive enzymes such as α-glucosidase. It has been shown that fermentation of these compounds by the gut microbiota leads to the production of short-chain fatty acids (SCFAs), which improve insulin sensitivity and stimulate the secretion of incretin hormones such as GLP-1. A literature review confirms that regular consumption of whole-grain products is associated with a reduced risk of T2D, whereas refining processes and excessive grain fragmentation lead to an increased glycemic index of products. Based on clinical guidelines and a narrative synthesis of the available literature, minimally processed whole-grain products were identified as a fundamental component of dietary therapy for diabetes, which is illustrated by the cereal product pyramid presented in the paper. This review involved a comprehensive literature search in PubMed, Scopus, and Web of Science using relevant keywords. Peer-reviewed articles, reviews, and meta-analyses (mainly 2000–2025) were included based on their relevance. Full article

Monitoring inland surface water quality is essential for water security, ecosystem conservation, public health, and sustainable water resource management. Although in situ measurements remain indispensable, they are often limited by high costs, restricted spatial coverage, low temporal frequency, and discontinuous monitoring networks. This study presents a systematic literature review, guided by the PRISMA 2020 framework, of empirical studies published between 2021 and 2025 on the integration of artificial intelligence (AI) and remote sensing (RS) for inland surface water quality monitoring. Searches were conducted in the Web of Science database, resulting in a final corpus of 367 peer-reviewed articles. Preliminary bibliometric characterization and qualitative content analysis were performed to identify sensors, platforms, AI paradigms, algorithms, estimated parameters, validation strategies, limitations, challenges, trends, and research gaps. The results show rapid growth in the field, with Sentinel-2 and Landsat-8 as the most recurrent sensors and multispectral data as the dominant spectral source. Machine learning approaches, especially Random Forest, Artificial Neural Networks, XGBoost, and Support Vector Machine, predominated, while deep learning, multi-source integration, hybrid models, and Explainable AI emerged as relevant trends. AI–RS integration shows strong potential to complement conventional monitoring, but persistent challenges remain regarding in situ data dependence, limited external and temporal validation, model transferability, generalization, uncertainty reporting, validation robustness, and interpretability. Full article

Background: Exercise adaptation is increasingly recognized as an immunometabolic process driven by coordinated interactions among inflammatory signaling, mitochondrial regulation, metabolic homeostasis, and recovery-associated physiology. Within this framework, NLRP3 inflammasome activation and PPARD-mediated metabolic signaling have emerged as biologically relevant pathways potentially involved in exercise-induced physiological adaptation. However, the contribution of regulatory genetic variations linking these pathways remains poorly characterized. Objective: To synthesize current evidence regarding the integration of NLRP3- and PPARD-related pathways in exercise immunometabolism and adaptive physiological responses to exercise, with particular emphasis on the regulatory variants NLRP3 rs10754558 and PPARD rs2267668 as potential contributors to interindividual variability in exercise adaptation. Methods: A structured narrative review complemented by exploratory systems-level in silico analyses was conducted using the PubMed, Scopus, and Web of Science databases until March 2026. Evidence related to exercise physiology, inflammatory regulation, metabolic adaptation, and exercise-associated phenotypes involving the NLRP3 and PPARD pathways was evaluated. Complementary analyses included functional annotation, protein–protein interaction network analysis, and pathway enrichment using STRING, Reactome, KEGG, Gene Ontology, and other publicly available genomic databases. Particular attention was given to the functional and regulatory context of rs10754558 and rs2267668 within the interconnected inflammatory and metabolic pathways relevant to exercise adaptation. Results: The reviewed evidence identified recurrent interactions among the inflammatory and metabolic pathways involved in exercise adaptation and recovery. NLRP3 rs10754558 and PPARD rs2267668 were identified as candidate regulatory variants potentially positioned at the interface between inflammatory responsiveness and metabolic flexibility, providing a biologically plausible framework for understanding the interindividual variability in exercise adaptation. Exploratory system-level analyses identified recurrent associations among inflammatory signaling, mitochondrial function, energy-sensing pathways, and metabolic regulation. These findings primarily reflect the functional annotations and system-level pathway associations identified through exploratory analyses. Conclusions: Current evidence supports a systems-level physiological framework in which inflammatory and metabolic pathways interact dynamically during exercise adaptation and recovery. NLRP3- and PPARD-related pathways, including the candidate regulatory variants rs10754558 and rs2267668, may contribute to interindividual variability in exercise-associated physiological responses and represent promising targets for future hypothesis-driven investigations in exercise immunometabolism, exercise genomics and precision exercise medicine. Full article

Sustainable land-use regulation in historical and cultural cities requires balancing heritage conservation, development demand, cropland retention, and urban–rural spatial restructuring. However, the stage-wise reorganization of urban–rural construction land under these coupled pressures remains insufficiently understood. Taking Jingzhou District, China, as a case study, this study uses land-use data from 2000, 2005, 2010, 2015, and 2020 and integrates stage-wise random-forest analysis, consistency-based interaction-network mining, and multi-scenario simulation within the intPLUS framework. Population, GDP, and areal-water distance layers were matched to the corresponding stage-terminal snapshots where applicable, whereas 2020 POI data were used as contemporary spatial-context proxies. From 2000 to 2020, urban industrial land (UIL) expanded from 16.63 to 46.42 km², increasing by approximately 179.1%, whereas rural settlements (RS) increased more moderately from 56.59 to 60.27 km², increasing by approximately 6.5%. The stage-wise RF and interaction-network results show that UIL and RS followed different spatial association structures, with stronger UIL self-reinforcement and stronger RS self-continuity in the later stage. Historical validation showed overall accuracy values of approximately 91% and Kappa values around 0.80, but FoM values remained relatively low, ranging from 0.098 to 0.176. Class-specific mapping accuracy was higher for RS (81.90–82.37%) than for UIL (55.20–66.93%), indicating a weaker performance in locating UIL change. Therefore, the 2035 simulations should be interpreted as parameter-conditioned regulatory comparisons rather than deterministic pixel-level forecasts. The scenario results indicate that the conservation-oriented limited growth was associated with the restricted UIL expansion and better cropland retention under the prescribed demand and constraint settings, while the RS reduction occurred only under explicit village-consolidation and construction-land quota reallocation assumptions. By distinguishing UIL and RS, this study provides differentiated regulation-oriented evidence for sustainable land-use governance in historical and cultural cities. Full article

The dynamic growth of tourism in Serbia has significantly reshaped the spatial structure of destinations, raising important issues related to sustainable development and spatial management. This study aims to examine the perceived effectiveness of spatial planning in tourism, based on tourists’ assessments of plan implementation and its visible implications for sustainable development. The research was conducted in Serbia in 2025 using a mixed-method approach, combining quantitative and qualitative analysis based on a survey of 208 valid respondents. The quantitative analysis included Spearman’s rank correlation and Z-test to examine relationships between variables and differences in attitudes, while qualitative insights were derived from open-ended responses. The results indicate a statistically significant positive correlation between the perceived implementation of spatial plans and the level of tourism development (Rs = 0.283, p < 0.001). However, the findings also reveal that 41.3% of respondents believe that tourism is only slightly considered in spatial plans, while 45.19% express negative attitudes toward the integration of tourism into planning processes. The study identifies key challenges, including weak cross-sector coordination, insufficient integration of tourism into spatial plans, and limited involvement of local communities. These findings highlight a critical gap between formal planning frameworks and their practical implementation. The main contribution of this research lies in providing empirical evidence from the perspective of tourists, a stakeholder group often overlooked in spatial planning studies, thereby addressing a gap in the literature on tourism–planning integration in Serbia. The results suggest that improving institutional coordination, strengthening participatory planning, and enhancing monitoring mechanisms are essential for achieving sustainable tourism development. The study offers targeted policy implications for aligning spatial planning with tourism development goals while preserving natural and cultural resources. Full article

Stroke is a leading cause of morbidity and long-term disability worldwide. This study evaluated the feasibility, safety, and preliminary clinical effects of phonotherapy (PHT) as an adjunct to standard care in patients with acute ischemic stroke. This prospective observational study enrolled 140 patients, who were assigned to receive either phonotherapy in addition to standard care (PHT group, n = 70) or standard care alone (control group, n = 70). Phonotherapy consisted of twice-daily 528 Hz sound stimulation administered for 3 months. Neurological (NIHSS), functional (mRS), and cognitive (MoCA) outcomes were assessed at days 10 and 90. At day 10, patients receiving PHT showed significantly better neurological, functional, and cognitive outcomes compared to the controls. However, these differences were not sustained at 90 days. Phonotherapy was not an independent predictor of favorable functional outcome at 90 days. Recurrent stroke occurred in three patients (4.3%) in the PHT group and nine (12.9%) in the control group (p = 0.07). No intervention-related adverse events were observed. Phonotherapy appears to be a safe adjunct intervention in acute ischemic stroke and may be associated with short-term improvements in selected outcomes. Overall, phonotherapy appeared safe as an adjunctive intervention in patients with acute ischemic stroke and showed possible short-term associations with improvements in selected outcomes, although these preliminary findings require confirmation in randomized controlled trials. Full article

Background/Objectives: Obesity remains a global health concern, and personalized prevention strategies that consider genetic predispositions can enhance existing strategies. Research suggests that variation in circadian rhythm-related genes, or clock genes, may influence obesity risk, in part through effects on dietary behaviour. However, associations between single-nucleotide polymorphisms (SNPs) in clock genes and dietary outcomes remain understudied, particularly in children. Therefore, we investigated cross-sectional associations between clock gene SNPs and dietary outcomes using baseline data from 226 adults (138 females, 88 males) aged 26–50 y and 168 children (90 females, 78 males) aged 2–6 y from the Guelph Family Health Study. Methods: DNA was extracted from saliva and genotyped using the Illumina Global Diversity Array, and dietary intake was assessed using the Automated Self-Administered 24 h Dietary Assessment Tool. Nine SNPs representing 8 clock genes were selected based on prior associations with dietary and obesity-related outcomes. Generalized Estimating Equations were used to test associations, adjusted for multiple comparisons with the Benjamini–Hochberg false discovery rate (FDR) procedure. Results: Ten nominal associations were identified (p < 0.05), and 2 remained significant after FDR correction (P_adj < 0.05); among children, rs2314339-T (NR1D1) was associated with a lower percentage of energy from protein (β = −2.4%, P_adj = 0.003) and rs11605924-A (CRY2) with higher energy intake (β = 118.0 kcal, P_adj = 0.044). Conclusions: Findings suggest that clock gene SNPs may influence dietary habits from early childhood. Future longitudinal and functional studies are needed to clarify whether these variants can inform precision nutrition strategies for obesity prevention. Full article

Mercury (Hg) is a major neurotoxicant and public health concern and gold mining is a significant source of Hg contamination in the Amazon. There, Indigenous peoples are vulnerable to this exposure. Individual susceptibility influences both internal mercury levels and related clinical outcomes. In this context, the GSTP1 gene stands out due to its role in detoxification of xenobiotics. The objectives were to assess the associations between: (1) Hg levels and neurotoxicity signs; (2) the GSTP1 rs1695 polymorphism and Hg levels; and (3) whether the GSTP1 rs1695 polymorphism modifies the effect of mercury on neurotoxicity signs. A cross-sectional study was conducted between April and May 2023, with 113 Paiter-Suruí Indigenous people. Sociodemographic and clinical data were collected using a validated methodology. Hair and oral mucosa cells were collected to assess Hg levels and the GSTP1 rs1695 polymorphism. Hg levels ranged from 0.1 μg/g to 6.5 μg/g (median = 1 μg/g, IQR = 1.43). Individuals with impaired memory and muscle strength had significantly higher mercury levels (β = 4.39 and β = 1.24). Carriers of the GSTP1AA genotype showed a 0.46-point reduction for each 1 μg/g increase in mean Hg levels, compared to individuals with the GSTP1GG genotype (β = −0.46). These results may support public policies by identifying priority groups for intervention based on genetic profiles. Full article

Remote sensing referring segmentation aims to extract the exact region of an object in an aerial image based on a natural language description, but it remains challenging because remote sensing scenes cover large areas, many objects look similar, and the descriptions are often long and detailed. Existing attention-based models are computationally expensive on large images and may underuse fine-grained language cues, which can lead to inaccurate or incomplete masks. To address this, we present MSMamba, an efficient framework built on a state space model for stable long-range context modeling over large spatial grids. We further strengthen language grounding by identifying descriptive words in the expression and using them to guide visual features from coarse localization to boundary refinement. In addition, we design a scale-aware decoding strategy that fuses multi-scale features with adaptive gating to better handle severe size variation and thin structures. Experiments on four public benchmarks show that MSMamba consistently improves segmentation quality. On RefSegRS, MSMamba improves Pr@0.8 on the test set by 25.53% and increases mIoU by 6.65%. On RRSIS-HR, MSMamba improves Pr@0.8 by 9.09% and increases mIoU by 3.02%. These results suggest that combining a state space model with structured language guidance and scale-aware fusion is a practical alternative to attention-only designs for remote sensing referring segmentation. Full article

Background/Objectives: Helplines are an important component of mental health support systems; however, limited research has examined burnout among helpline workers. This exploratory pilot study investigated the relationships between job demands, job and personal resources, and burnout within the Job Demands–Resources (JD-R) framework. Methods: A cross-sectional online study was conducted among 73 helpline workers. Burnout was assessed using the Burnout Assessment Tool (BAT). Job demands were categorized as topic-related, client-related, and service-related. Data were analysed using Spearman correlations and hierarchical regression analyses. Results: Job demands were positively associated with burnout (r_s = 0.41, p < 0.001), while job resources were negatively associated with burnout (r_s = −0.30, p < 0.01). Regression analyses showed that job demands and resources explained 50% of the variance in overall burnout (R² = 0.50, p < 0.001). Service-related demands emerged as the strongest predictor of burnout (β = 0.70, p < 0.001) and consistently predicted exhaustion (β = 0.60, p < 0.001), mental distance (β = 0.48, p < 0.001), cognitive impairment (β = 0.52, p < 0.001), and emotional impairment (β = 0.61, p < 0.001). Organizational resources were negatively associated with mental distance (β = −0.31, p < 0.01), whereas topic-related demands were not significant predictors. Conclusions: The findings highlight the importance of differentiating types of job demands in understanding burnout among helpline workers. Service-related demands appeared to be more strongly associated with burnout than topic- or client-related demands, suggesting that structural aspects of helpline work may be particularly relevant for worker well-being. Full article

Rapid and reliable detection methods are essential for routine monitoring of environmental hygiene on farms. This pilot study evaluated luminometers (LUM) and mobile flow cytometer (MFC) for assessment of surface organic contamination in farrowing units. The study was conducted on two pig farms after animal removal prior to sanitation, with sampling performed at heated pads, pen walls, and corridors. ATP measurements were carried out using three luminometers (Clean-Trace™ LM1, EnSure, and SystemSURE Plus), and residual particles were detected using a mobile flow cytometer (Cytoquant). Microbiological cultivation (TMC 36 °C) was additionally included. Significant differences in log RLU values were observed between LUM, with large effect sizes indicating a substantial influence of device type on RLU values. A high correlation was confirmed only between EnSure and SystemSURE Plus (r_s = 0.81–1.00; p < 0.05), and no relationship was confirmed between LUM and MFC (r_s = −0.49–0.77; p > 0.05). Correlations between rapid detection methods and microbiological cultivation were inconsistent. Corridors demonstrated the highest microbiological contamination, whereas MFC identified heated pads as sites with increased residual particulate contamination. The results indicate that LUM, MFC, and microbiological cultivation characterize different dimensions of environmental contamination and should therefore be interpreted as complementary rather than interchangeable methods. Full article

Background: Vitamin D, through its role in antimicrobial peptide (AMP) expression, may influence innate immunity and inflammation in urinary tract infections (UTIs). This study evaluated its role in patients with vesicoureteral reflux (VUR) and its contribution to the pathophysiology of reflux nephropathy (RN). Methods: We conducted a cross-sectional observational study of 25 pediatric patients with VUR, representing a subgroup analysis of a larger cohort examined in a previous study. We determined patients’ vitamin D status, correlated it with recurrent UTIs and RS, and explored its relationship with urinary LL-37, NGAL, and IL-6 levels as markers of innate immune function. Results: Serum vitamin D levels ranged from 10.7 to 123.2 ng/mL (mean 39.5 ng/mL); 12% had deficiency and 20% had insufficient levels. Low vitamin D levels were detected in patients with more than five acute pyelonephritis (APNs), with a mean value classified as insufficient (27.3 ng/mL). Patients with RS had a lower mean vitamin D level compared to those without (30.51 ng/mL vs. 41.23 ng/mL), though the difference was not statistically significant (p = 0.39). No significant associations were found between vitamin D and urinary IL-6 or NGAL levels. A strong positive correlation was observed between vitamin D and urinary LL-37/creatinine (r = 0.78, r² = 0.61). Conclusions: Vitamin D appears to influence the frequency of UTIs and the development of RS, primarily by modulating LL-37 secretion, suggesting a possible role in the pathophysiology of RN. Full article

Background: Gastrointestinal (GI) cancers, particularly colorectal, gastric, and liver cancers, account for a major global burden of incidence and mortality and remain important targets for genetic susceptibility research. Long non-coding RNAs (lncRNAs) can regulate gene expression and are increasingly studied in carcinogenesis. Numerous case–control studies have investigated associations between lncRNA polymorphisms and cancer risk, but findings are inconsistent. This study systematically evaluated the association between lncRNA single nucleotide polymorphisms (SNPs) and GI cancer susceptibility. Methods: A systematic literature search from Embase, Medline, Scopus, and Web of Science databases identified 174 potentially extractable studies. Eligible studies were case–control or cross-sectional studies published up to 8 May 2026; case reports, reviews, and meta-analyses were excluded. After screening for identical cancer type, identical SNP, and sufficient statistical data, only variants supported by at least three independent case–control studies were eligible for meta-analysis. Seven SNPs across six lncRNAs, comprising 23 studies (15,131 cases and 20,969 controls), were selected. Because of the limited number of eligible studies, subgroup analyses could not be performed consistently. Odds ratios (ORs) with 95% confidence intervals (CIs) were assessed under allelic, dominant, and recessive genetic models using fixed- or random-effects models according to heterogeneity. Results: In the primary analyses restricted to homogenous Chinese populations, H19 rs3024270 was significantly associated with hepatocellular carcinoma under allelic (OR = 1.22, 95% CI: 1.05–1.42, p = 0.01) and dominant models (OR = 1.22, 95% CI: 1.03–1.45, p = 0.02). Exploratory analyses including mixed populations identified additional associations, with the strongest observed for MEG3 rs7158663 and colorectal cancer, showing significant risk elevation under allelic (OR = 1.42, 95% CI: 1.25–1.63, p < 0.00001), dominant (OR = 1.42, 95% CI: 1.20–1.68, p < 0.0001), and recessive models (OR = 1.98, 95% CI: 1.46–2.68, p < 0.0001). PRNCR1 rs16901946 showed a significant association with gastric cancer under the dominant model (OR = 1.20, 95% CI: 1.02–1.41, p = 0.03), while GAS5 rs145204276 demonstrated a recessive-model association with gastric cancer (OR = 1.30, 95% CI: 1.16–1.46, p < 0.0001). In contrast, GAS5 rs145204276 in colorectal cancer; H19 rs2839698 and MALAT1 rs619586 in hepatocellular carcinoma yielded heterogeneous or unstable pooled estimates. Findings should be interpreted cautiously due to the limited number of studies, heterogeneity, and potential publication bias. Conclusions: Among the primary analyses, H19 rs3024270 showed the most consistent association with HCC susceptibility. Exploratory analyses identified candidate variants, including MEG3 rs7158663, PRNCR1 rs16901946, and GAS5 rs145204276. Population-specific effects and study heterogeneity remain important limitations. PROSPERO registration number for this study: CRD42023389742. Full article