Search Results (1,815)

This research demonstrates the potential of plant waste cellulose as a remarkable biomaterial for multilayer tablet formulation. Rice husks (RC) and orange peels (OC) were used as cellulose sources and characterized for a comparison with commercial cellulose. The FTIR characterization shows minimal differences in their chemical components, making them equivalent for compression into tablets containing ibuprofen. TGA measurements indicate that the RC is slightly better for multilayer formulations due to its favorable degradation profile. This is corroborated by an XRD analysis that reveals its higher crystalline fraction (~55%). The use of a heat press at combined high pressures and temperatures allows the layer-by-layer tablet formulation of ibuprofen, taken as a model drug. Additionally, this study compares the release profile of three types of tablets compressed with cellulose: mixed (MIX), two-layer (BL), and three-layer (TL). The MIX tablet shows a profile like that of conventional ibuprofen tablets. Although both BL and TL tablets significantly reduce their release percentage in the first hours, the TL ones have proven to be better in the long run. In fact, formulations made of extracted cellulose sandwiching ibuprofen display a zero-order release profile and prolonged release since the drug release amounts to ~70% after 120 h. This makes the TL formulations ideal for maintaining the therapeutic effect of the drug and improving patients’ wellbeing and compliance while reducing adverse effects. Full article

Rice seed storage proteins (SSPs) play a critical role in determining the nutritional quality, cooking properties, and digestibility of rice. To enhance seed quality, CRISPR/Cas9 genome editing was applied to modify SSP composition by targeting genes encoding 13 kDa prolamins and type A glutelins. Three CRISPR/Cas9 constructs were designed: one specific to the 13 kDa prolamin subfamily and two targeting conserved GluA glutelin regions. Edited T₀ and T₁ lines were generated and analyzed using InDel analysis, SDS-PAGE, Bradford assay, and RP-HPLC. Insertions were more frequent than deletions, accounting for 56% and 74% of mutations in prolamin and glutelin genes, respectively. Editing efficiency varied between sgRNAs. All lines with altered protein profiles contained InDels in target genes. SDS-PAGE confirmed the absence or reduction in bands corresponding to 13 kDa prolamins or GluA subunits, showing consistent profiles among lines carrying the same construct. Quantification revealed significant shifts in SSP composition, including increased albumin and globulin content. Prolamin-deficient lines showed reduced prolamins, while GluA-deficient lines exhibited increased prolamins. Total protein content was significantly elevated in all edited lines, suggesting enrichment in lysine-rich fractions. These findings demonstrate that CRISPR/Cas9-mediated editing of SSP genes can effectively reconfigure the rice protein profile and enhance its nutritional value. Full article

This study investigated the impact of nitrogen (N) fertilization on the yield and grain filling (GF) characteristics of two conventional japonica rice varieties with distinct panicle types: Yangchan 3501 (large-panicle: spikelets per panicle $> 150$) and Nangeng 46 (medium-panicle: $100 <$ spikelets per panicle $< 150$). Field experiments were conducted over two growing seasons (2022–2023) with three N application rates (T1: 225 kg ha⁻¹, T2: 270 kg ha⁻¹, T3: 315 kg ha⁻¹). Key measurements included tiller dynamics, panicle composition, GF parameters modeled using the Richards equation, and enzyme activities related to nitrogen metabolism (Fd-GOGAT, NR) and carbohydrate transport (α-amylase, SPS). Results showed that the yield increased with higher N levels for both varieties, with Yangchan 3501 achieving higher yields primarily through increased grains per panicle (15.65% rise under T3 vs. T1), while Nangeng 46 relied on panicle number (8.83% increase under T3 vs. T1). Nitrogen application enhanced Fd-GOGAT and NR activities, prolonging photosynthesis and improving GF rates, particularly in the inferior grains of Yangchan 3501 during middle and late stages. However, a high N reduced seed-setting rates and 1000-grain weight, with larger panicle types exhibiting a greater sensitivity to N-induced changes in branch structure and assimilate allocation. This study highlights that optimizing N management can improve nitrogen-metabolism enzyme activity and GF efficiency, especially in large-panicle rice, while medium-panicle types require higher N inputs to maximize panicle number. These findings provide actionable insights for achieving high yields and efficient nutrient use in conventional rice cultivation. Full article

Glutathione peroxidase (GPX) is crucial in mediating plant responses to abiotic stresses. In this study, bioinformatics methods were used to identify the GPX gene family in quinoa. A total of 15 CqGPX genes were identified at the quinoa genome level and conducted preliminary analysis on their protein characteristics, chromosome distribution, gene structure, conserved domain structure, cis-acting elements, and expression patterns. Phylogenetic analysis showed that the GPX genes of quinoa, Arabidopsis, soybean, rice, and maize were divided into three groups. Most of the CqGPXs had the three characteristic conserved motifs and other conserved sequences and amino acid residues. Six types of cis-acting elements were identified in the CqGPX gene promoter, with stress and hormone response-related cis-acting elements constituting the two main categories. Additionally, the expression patterns of CqGPX genes across various tissues and their responses to treatments with NaCl, PEG, CdCl₂, and H₂O₂ were also investigated. The qRT-PCR results showed significant differences in the expression levels of the CqGPX genes under stress treatment at different time points. Consistently, the activity of glutathione peroxidase enzymes increased under stresses. Heterologous expression of CqGPX4 and CqGPX15 conferred stress tolerance to E. coli. This study will provide a reference for exploring the function of CqGPX genes. Full article

Cadmium (Cd) contamination in rice (Oryza sativa L.) poses serious health risks for human, necessitating effective mitigation strategies. This study investigated the effects of Cd stress on iron (Fe), manganese (Mn), zinc (Zn), and Cd accumulation and translocation in rice varieties with high (MY46) or low (ZS97B) Cd accumulation capacities grown in acidic and alkaline soils. Results demonstrated that Cd stress significantly inhibited plant growth, reducing plant height, shoot biomass, and grain yield in both soil types. Cd accumulation increased in roots, shoots, and grains, while Fe, Mn, and Zn concentrations decreased markedly. Molecular analysis revealed upregulation of metal transporter genes (OsIRT1, OsNRAMP1, OsNRAMP5) and the vacuolar sequestration gene (OsHMA3) in roots under Cd exposure. The translocation factor (TF) values of Mn and Zn from root to shoot were reduced in acidic soils, whereas Mn and Zn TFs exhibited an increasing trend in alkaline soils despite Cd exposure. Furthermore, correlation analyses indicated Mn and Zn play crucial roles in suppressing Cd accumulation in both acidic and alkaline soils. These findings provide critical insights for developing soil-specific strategies to reduce Cd accumulation in rice through micronutrient management. Full article

GOLDEN2-LIKEs (GLKs) are important transcription factors for the chloroplast development influencing photosynthesis, nutrition, senescence, and stress response in plants. Sunflower (Helianthus annuus) is a highly photosynthetic plant; here, a GLK-homologues gene HaGLK was identified from the sunflower genome by bioinformatics. To analyze the bio-function of HaGLK, transgenic rice plants overexpressing HaGLK (HaGLK-OE) were constructed and characterized via phenotype. Compared to the wild-type control rice variety Zhonghua 11 (ZH11), the HaGLK-OE lines exhibited increased photosynthetic pigment contents, higher net photosynthetic rates, and enlarged chloroplast area; meanwhile, genes involved in both photosynthesis and chlorophyll biosynthesis were also significantly up-regulated. Significantly, the HaGLK-OE plants showed a 12–13% increase in yield per plant. Additionally, the HaGLK-OE plants were demonstrated to have improved salt and drought tolerance compared to the control ZH11. Our results indicated that the HaGLK gene could play multiple roles in photosynthesis and stress response in rice, underscoring its potential value for improving crop productivity and environmental adaptability in breeding. Full article

With increasing popularity of food delivery services, the microbial safety of transported meals should be ensured. An effect of the type of a meal (cooked rice; mashed potatoes; mushroom sauce), inner primary packaging (sugarcane bagasse [SB] tray; polypropylene [PP] tray), secondary container (polyester/polyethylene foam/aluminum foil [PPA] bag; PP box) on the time interval of the internal hot ready-to-eat (RTE) meal temperature decrease to the value critical for Bacillus cereus growth (40 °C) was tested during a simulated delivery; in aliquot samples of the same meals, B. cereus growth was quantified presuming a natural contamination of the meals. Type of a meal had no effect on the tested time interval (p > 0.05). Packaging a meal in the PP tray as compared to the SB tray and inserting primary trays into the PP box instead of PPA bag delayed (p < 0.05) the internal meal temperature decrease by 50 and 15 min, respectively. Average B. cereus counts in the naturally contaminated meals after the four-hour culturing at 40 °C was 2.99 log CFU·g⁻¹. It was concluded that a hot RTE meal delivered up to four hours under the tested conditions is not likely to facilitate B. cereus growth above unacceptable levels. Full article

Glass microspheres are essential components in horizontal road markings due to their retroreflective properties, enhancing visibility and safety under low-light conditions. Traditionally produced from soda-lime glass made with high-purity silica from sand, their manufacturing raises environmental concerns amid growing global sand scarcity. This study explores the viability of rice husk ash (RHA)—a high-silica byproduct of rice processing—as a sustainable raw material for microsphere fabrication. A glass composition containing 70 wt% SiO₂ was formulated using RHA and melted at 1500 °C. Microspheres were produced through flame spheroidization and characterized following the Brazilian standard NBR 16184:2021 for Type IB beads. The RHA-derived microspheres exhibited high sphericity, appropriate size distribution (63–300 μm), density of 2.42 g/cm³, and the required acid resistance. UV-Vis analysis confirmed their optical transparency, and the refractive index was measured as 1.55 ± 0.03. Retroreflectivity tests under standardized conditions revealed performance comparable to commercial counterparts. These results demonstrate the technical feasibility of replacing conventional silica with RHA in glass microsphere production, aligning with circular economy principles and promoting sustainable infrastructure. Given Brazil’s significant rice production and corresponding RHA availability, this approach offers both environmental and socio-economic benefits for road safety and material innovation. Full article

Late sowing and spring low temperatures have a great impact on the growth and maturation of wheat in the rice–wheat rotation region. In order to analyze the impacts of cold stress in February in early spring on yield formation and agronomic traits of wheat on different sowing dates, a controlled pot experiment was performed using the widely promoted and applied spring-type wheat variety Yangmai23 (YM23). The yield of wheat treated with late sowing date II (SDII, 21 November) and overly late sowing date III (SDIII, 9 December) were both lower than that of wheat sown on the suitable date I (SDI, 1 November). The yield of late-sown wheat decreased by 40.82% for SDII and by 66.77% for SDIII, compared with SDI, and these three treatments of wheat all grew under the natural conditions as the control treatments. The plant height, stem diameter of the internode below the ear, flag leaf length and area, and total awn length of the spike, as well as the spike length of late-sown wheat, were all significantly lower than those of wheat in SDI treatment. Early spring low temperatures exacerbated the decline in yield of wheat sown on different dates, to some extent. Despite showing higher net photosynthetic rate, stomatal conductance, and transpiration rate in flag leaves of the SDIII treatment under low-temperature stress than those of the other treatments at anthesis, overly late sowing led to minimal leaf area, shorter plant height, fewer tillers, and smaller ears, ultimately resulting in the lowest yield. Our study suggested that additional focus and some regulation techniques are needed to be studied further to mitigate the combined negative impacts of late sowing and low-temperature stress in early spring on wheat production. Full article

Sheath blight (ShB), caused by the necrotrophic fungus Rhizoctonia solani (R. solani), poses severe threats to global rice production. Developing a resistant variety with an ShB-resistance gene is one of most efficient and economical approaches to control the disease. Here, we identified a highly conserved chloroplast-localized stem-loop-binding protein encoding gene (OsCSP41b), which shows great potential in developing an ShB-resistant variety. OsCSP41b-knockout mutants exhibit chlorotic leaves and increased ShB susceptibility, whereas OsCSP41b-overexpressing lines (CSP41b-OE) display significantly enhanced resistance to R. solani, as well as to drought, and salinity stresses. Notably, CSP41b-OE lines present a completely comparable grain yield to the wild type (WT). Transcriptomic analyses reveal that chloroplast transcripts and photosynthesis-associated genes maintain observably elevated stability in CSP41b-OE plants versus WT plants following R. solani infection, which probably accounts for the enhanced ShB resistance of CSP41b-OE. Our findings nominate the OsCSP41b gene as a promising molecular target for developing a rice variety with stronger resistance to both R. solani and multi-abiotic stresses. Full article

Rice wine, as a traditional fermented beverage, has its quality and flavor influenced by a combination of multiple factors. This review provides an overview of the key aspects of rice wine production, including raw material selection and processing, the regulation of quality by brewing techniques, the mechanisms of microbial community interaction during fermentation, and the types and formation mechanisms of major compounds in rice wine (including flavor compounds and non-volatile components). The study highlights that different raw materials and processing methods significantly impact the fundamental flavor profile of rice wine, while fermentation conditions and dynamic changes in microbial communities determine its flavor complexity and stability. Additionally, this review examines various factors affecting the quality and flavor of rice wine, such as fermentation environment, microbial metabolism, and control of harmful substances, and summarizes modern research and technological advancements, emphasizing the potential of digital and intelligent technologies in enhancing the quality and safety of rice wine. Finally, future research directions are proposed to promote modernization and quality improvement of the rice wine industry. Full article

Micronutrient deficiencies adversely affect human health and pose a significant global threat. Enhancing the accumulation of micronutrients in the edible parts of crops through genetic breeding is a promising strategy to mitigate micronutrient deficiencies in humans. FW2.2-like (FWL) genes play crucial roles in regulating heavy metal homeostasis in plants. We previously obtained two allelic mutants for each of the rice OsFWL1 (osfwl1a and osfwl1b) and OsFWL2 (osfwl2a and osfwl2b) genes. In this study, we showed that disruption of either OsFWL1 or OsFWL2 significantly enhanced the accumulation of metallic micronutrients in brown rice. Compared with that in the wild type, the iron (Fe) concentration in brown rice was higher in the osfwl1a (+166.7%), osfwl1b (+24.3%), and osfwl2a (+99.2%) mutants; the manganese (Mn) concentration was elevated in all four mutants (+25.1% to 35.6%); the copper (Cu) concentration increased in osfwl2a (+31.0%) and osfwl2b (+29.0%); and the zinc (Zn) concentration increased in osfwl2a (+10.2%). Additionally, disruption of OsFWL1 or OsFWL2 affected the homeostasis of metallic micronutrients in seedlings. Transcriptome analysis suggested that OsFWL1 and OsFWL2 might regulate cell wall polysaccharide metabolism and the expression of heavy metal transporter genes. Protein interaction analysis revealed that OsFWL1 interacted with OsFWL2 on the cell membrane. These findings suggest that OsFWL1 and OsFWL2 can serve as genetic biofortification tools to increase the concentrations of metallic micronutrients in rice grains. Full article

Ancient grains, including wild rice, millet, fonio, teff, quinoa, amaranth, and sorghum, are re-emerging as vital components of modern diets due to their dense nutritional profiles and diverse health-promoting bioactive compounds. Rich in high-quality proteins, dietary fiber, essential micronutrients, and a broad spectrum of bioactive compounds such as phenolic acids, flavonoids, carotenoids, phytosterols, and betalains, these grains exhibit antioxidant, anti-inflammatory, antidiabetic, cardioprotective, and immunomodulatory properties. Their health-promoting effects are underpinned by multiple interconnected mechanisms, including the reduction in oxidative stress, modulation of inflammatory pathways, regulation of glucose and lipid metabolism, support for mitochondrial function, and enhancement of gut microbiota composition. This review provides a comprehensive synthesis of the essential nutrients, phytochemicals, and functional properties of ancient grains, with particular emphasis on the nutritional and molecular mechanisms through which they contribute to the prevention and management of chronic diseases such as cardiovascular disease, type 2 diabetes, obesity, and metabolic syndrome. Additionally, it highlights the growing application of ancient grains in functional foods and nutrition-sensitive dietary strategies, alongside the technological, agronomic, and consumer-related challenges limiting their broader adoption. Future research priorities include well-designed human clinical trials, standardization of compositional data, innovations in processing for nutrient retention, and sustainable cultivation to fully harness the health, environmental, and cultural benefits of ancient grains within global food systems. Full article

The increasing presence of ECs in aquatic environments has drawn significant attention to the need for innovative, accessible, and sustainable solutions in wastewater treatment. This review provides a comprehensive overview of the use of agricultural residues—often discarded and undervalued—as raw materials for the development of efficient bioadsorbents. Based on a wide range of recent studies, this work presents various types of materials, such as rice husks, sugarcane bagasse, and açaí seeds, that can be transformed through thermal and chemical treatments into advanced bioadsorbents capable of removing pharmaceuticals, pesticides, dyes, and in some cases, even addressing highly persistent pollutants such as PFASs. The main objectives of this review are to (1) assess agricultural-residue-derived bioadsorbents for the removal of ECs; (2) examine physical and chemical modification techniques that enhance adsorption performance; (3) evaluate their scalability and applicability in real-world treatment systems. The review also highlights key adsorption mechanisms—such as π–π interactions, hydrogen bonding, and ion exchange—alongside the influence of parameters like pH and ionic strength. The review also explores the kinetic, isothermal, and thermodynamic aspects of the adsorption processes, highlighting both the efficiency and reusability potential of these materials. This work uniquely integrates microwave-assisted pyrolysis, magnetic functionalization, and hybrid systems, offering a roadmap for sustainable water remediation. Finally, comparative performance analyses, applications using real wastewater, regeneration strategies, and the integration of these bioadsorbents into continuous treatment systems are presented, reinforcing their promising role in advancing sustainable water remediation technologies. Full article

In the context of efforts to reduce greenhouse gas emissions in the building sector, the reintegration of traditional earthen construction into modern architectural and renovation practices offers a sustainable alternative. To address the mechanical and water-resistance limitations of adobe bricks, the use of agricultural waste—such as rice husk—is increasingly being explored. This experimental study evaluates the effects of rice husk addition on the mechanical, hygrothermal, and acoustic properties of adobe bricks. Two soil types—one siliceous and one calcareous—were combined with 1, 2, and 3 wt% rice husk to produce bio-based earthen bricks. The influence of rice husk was found to depend strongly on the soils’ mineralogical and granulometric characteristics. The most significant improvements were in hygrothermal performance: at 3 wt%, thermal conductivity was reduced by up to 35% for calcareous soil and 20% for siliceous soil, indicating enhanced insulation. Specific heat capacity also increased with husk content, suggesting better thermal inertia. The moisture buffering capacity, already high in raw soils, is further improved due to increased surface porosity. Mechanically, rice husk incorporation had mixed effects: a modest increase in compressive strength was observed in siliceous soil at 1 wt%, while calcareous soil showed slight improvement at 3 wt%. Acoustic performance remained low across all samples, with minimal gains attributed to limited macro-porosity. These findings highlight the importance of soil composition in optimizing rice husk dosage and suggest promising potential for rice husk-stabilized adobe bricks, especially in thermally demanding environments. Full article