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22 pages, 34153 KiB  
Article
Study on Lithospheric Tectonic Features of Tianshan and Adjacent Regions and the Genesis Mechanism of the Wushi Ms7.1 Earthquake
by Kai Han, Daiqin Liu, Ailixiati Yushan, Wen Shi, Jie Li, Xiangkui Kong and Hao He
Remote Sens. 2025, 17(15), 2655; https://doi.org/10.3390/rs17152655 (registering DOI) - 31 Jul 2025
Abstract
In this study, we analyzed the lithospheric seismic background of the Tianshan and adjacent areas by combining various geophysical methods (effective elastic thickness, time-varying gravity, apparent density, and InSAR), and explored the genesis mechanism of the Wushi Ms7.1 earthquake as an example, which [...] Read more.
In this study, we analyzed the lithospheric seismic background of the Tianshan and adjacent areas by combining various geophysical methods (effective elastic thickness, time-varying gravity, apparent density, and InSAR), and explored the genesis mechanism of the Wushi Ms7.1 earthquake as an example, which led to the following conclusions: (1) The effective elastic thickness (Te) of the Tianshan lithosphere is low (13–28 km) and weak, while the Tarim and Junggar basins have Te > 30 km with high intensity, and the loads are all mainly from the surface (F < 0.5). Earthquakes occur mostly in areas with low values of Te. (2) Medium and strong earthquakes are prone to occur in regions with alternating positive and negative changes in the gravity field during the stage of large-scale reverse adjustment. It is expected that the risk of a moderate-to-strong earthquake occurring again in the vicinity of the survey area between 2025 and 2026 is relatively high. (3) Before the Wushi earthquake, the positive and negative boundaries of the apparent density of the crust at 12 km shifted to be approximately parallel to the seismic fault, and the earthquake was triggered after undergoing a “solidification” process. (4) The Wushi earthquake is a leptokurtic strike-slip backwash type of earthquake; coseismic deformation shows that subsidence occurs in the high-visual-density zone, and vice versa for uplift. The results of this study reveal the lithosphere-conceiving environment of the Tianshan and adjacent areas and provide a basis for regional earthquake monitoring, early warning, and post-disaster disposal. Full article
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22 pages, 30259 KiB  
Article
Controlling Effects of Complex Fault Systems on the Oil and Gas System of Buried Hills: A Case Study of Beibuwan Basin, China
by Anran Li, Fanghao Xu, Guosheng Xu, Caiwei Fan, Ming Li, Fan Jiang, Xiaojun Xiong, Xichun Zhang and Bing Xie
J. Mar. Sci. Eng. 2025, 13(8), 1472; https://doi.org/10.3390/jmse13081472 - 31 Jul 2025
Abstract
Traps are central to petroleum exploration, where hydrocarbons accumulate during migration. Reservoirs are likewise an essential petroleum system element and serve as the primary medium for hydrocarbon storage. The buried hill is a geological formation highly favorable for reservoir development. However, the factors [...] Read more.
Traps are central to petroleum exploration, where hydrocarbons accumulate during migration. Reservoirs are likewise an essential petroleum system element and serve as the primary medium for hydrocarbon storage. The buried hill is a geological formation highly favorable for reservoir development. However, the factors influencing hydrocarbon accumulation in buried hill reservoirs are highly diverse, especially in areas with complex, active fault systems. Fault systems play a dual role, both in the formation of reservoirs and in the migration of hydrocarbons. Therefore, understanding the impact of complex fault systems helps enhance the exploration success rate of buried hill traps and guide drilling deployment. In the Beibuwan Basin in the South China Sea, buried hill traps are key targets for deep-buried hydrocarbon exploration in this faulted basin. The low level of exploration and research in buried hills globally limits the understanding of hydrocarbon accumulation conditions, thereby hindering large-scale hydrocarbon exploration. By using drilling data, logging data, and seismic data, stress fields and tectonic faults were restored. There are two types of buried hills developed in the Beibuwan Basin, which were formed during the Late Ordovician-Silurian period and Permian-Triassic period, respectively. The tectonic genesis of the Late Ordovician-Silurian period buried hills belongs to magma diapirism activity, while the tectonic genesis of the Permian-Triassic period buried hills belongs to reverse thrust activity. The fault systems formed by two periods of tectonic activity were respectively altered into basement buried hills and limestone buried hills. The negative structural inversion controls the distribution and interior stratigraphic framework of the deformed Carboniferous strata in the limestone buried hill. The faults and derived fractures of the Late Ordovician-Silurian period and Permian-Triassic period promoted the diagenesis and erosion of these buried hills. The faults formed after the Permian-Triassic period are not conducive to calcite cementation, thus facilitating the preservation of the reservoir space formed earlier. The control of hydrocarbon accumulation by the fault system is reflected in two aspects: on the one hand, the early to mid-Eocene extensional faulting activity directly controlled the depositional process of lacustrine source rocks; on the other hand, the Late Eocene-Oligocene, which is closest to the hydrocarbon expulsion period, is the most effective fault activity period for connecting Eocene source rocks and buried hill reservoirs. This study contributes to understanding of the role of complex fault activity in the formation of buried hill traps within hydrocarbon-bearing basins. Full article
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13 pages, 1031 KiB  
Article
MITS: A Quantum Sorcerer’s Stone for Designing Surface Codes
by Avimita Chatterjee, Debarshi Kundu and Swaroop Ghosh
Entropy 2025, 27(8), 812; https://doi.org/10.3390/e27080812 - 29 Jul 2025
Viewed by 114
Abstract
In the evolving field of quantum computing, optimizing Quantum Error Correction (QEC) parameters is crucial due to the varying types and amounts of physical noise across quantum computers. Traditional simulators use a forward paradigm to derive logical error rates from inputs like code [...] Read more.
In the evolving field of quantum computing, optimizing Quantum Error Correction (QEC) parameters is crucial due to the varying types and amounts of physical noise across quantum computers. Traditional simulators use a forward paradigm to derive logical error rates from inputs like code distance and rounds, but this can lead to resource wastage. Adjusting QEC parameters manually with tools like STIM is often inefficient, especially given the daily fluctuations in quantum error rates. To address this, we introduce MITS, a reverse engineering tool for STIM that automatically determines optimal QEC settings based on a given quantum computer’s noise model and a target logical error rate. This approach minimizes qubit and gate usage by precisely matching the necessary logical error rate with the constraints of qubit numbers and gate fidelity. Our investigations into various heuristics and machine learning models for MITS show that XGBoost and Random Forest regressions, with Pearson correlation coefficients of 0.98 and 0.96, respectively, are highly effective in this context. Full article
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14 pages, 3224 KiB  
Article
Impact of Charge Carrier Trapping at the Ge/Si Interface on Charge Transport in Ge-on-Si Photodetectors
by Dongyan Zhao, Yali Shao, Shuo Zhang, Tanyi Li, Boming Chi, Yaxing Zhu, Fang Liu, Yingzong Liang and Sichao Du
Electronics 2025, 14(15), 2982; https://doi.org/10.3390/electronics14152982 - 26 Jul 2025
Viewed by 168
Abstract
The performance of optoelectronic devices is affected by various noise sources. A notable factor is the 4.2% lattice mismatch at the Ge/Si interface, which significantly influences the efficiency of Ge-on-Si photodetectors. These noise sources can be analyzed by examining the impact of the [...] Read more.
The performance of optoelectronic devices is affected by various noise sources. A notable factor is the 4.2% lattice mismatch at the Ge/Si interface, which significantly influences the efficiency of Ge-on-Si photodetectors. These noise sources can be analyzed by examining the impact of the Ge/Si interface and deep traps on dark and photocurrents. This study evaluates the impact of these charge traps on key photodetector performance metrics, including responsivity, photo-to-dark current ratio, noise equivalent power (NEP), and specific detectivity (D*). The trapping effects on charge transport under both forward and reverse bias conditions are monitored through hysteresis analysis. When illuminated with an unmodulated 1550 nm laser, all the key performance metrics exhibit maximum variations at a specific reverse bias. This critical bias marks the transition from saturated to exponential charge transport regimes, where intensified electric fields enhance trap-assisted recombination and thus maximize metric fluctuations. Full article
(This article belongs to the Section Optoelectronics)
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22 pages, 5346 KiB  
Article
Numerical Study of Stud Welding Temperature Fields on Steel–Concrete Composite Bridges
by Sicong Wei, Han Su, Xu Han, Heyuan Zhou and Sen Liu
Materials 2025, 18(15), 3491; https://doi.org/10.3390/ma18153491 - 25 Jul 2025
Viewed by 302
Abstract
Non-uniform temperature fields are developed during the welding of studs in steel–concrete composite bridges. Due to uneven thermal expansion and reversible solid-state phase transformations between ferrite/martensite and austenite structures within the materials, residual stresses are induced, which ultimately degrades the mechanical performance of [...] Read more.
Non-uniform temperature fields are developed during the welding of studs in steel–concrete composite bridges. Due to uneven thermal expansion and reversible solid-state phase transformations between ferrite/martensite and austenite structures within the materials, residual stresses are induced, which ultimately degrades the mechanical performance of the structure. For a better understanding of the influence on steel–concrete composite bridges’ structural behavior by residual stress, accurate simulation of the spatio-temporal temperature distribution during stud welding under practical engineering conditions is critical. This study introduces a precise simulation method for temperature evolution during stud welding, in which the Gaussian heat source model was applied. The simulated results were validated by real welding temperature fields measured by the infrared thermography technique. The maximum error between the measured and simulated peak temperatures was 5%, demonstrating good agreement between the measured and simulated temperature distributions. Sensitivity analyses on input current and plate thickness were conducted. The results showed a positive correlation between peak temperature and input current. With lower input current, flatter temperature gradients were observed in both the transverse and thickness directions of the steel plate. Additionally, plate thickness exhibited minimal influence on radial peak temperature, with a maximum observed difference of 130 °C. However, its effect on peak temperature in the thickness direction was significant, yielding a maximum difference of approximately 1000 °C. The thermal influence of group studs was also investigated in this study. The results demonstrated that welding a new stud adjacent to existing ones introduced only minor disturbances to the established temperature field. The maximum peak temperature difference before and after welding was approximately 100 °C. Full article
(This article belongs to the Section Construction and Building Materials)
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21 pages, 4565 KiB  
Article
Experimental Study of Two-Bite Test Parameters for Effective Drug Release from Chewing Gum Using a Novel Bio-Engineered Testbed
by Kazem Alemzadeh and Joseph Alemzadeh
Biomedicines 2025, 13(8), 1811; https://doi.org/10.3390/biomedicines13081811 - 24 Jul 2025
Viewed by 360
Abstract
Background: A critical review of the literature demonstrates that masticatory apparatus with an artificial oral environment is of interest in the fields including (i) dental science; (ii) food science; (iii) the pharmaceutical industries for drug release. However, apparatus that closely mimics human [...] Read more.
Background: A critical review of the literature demonstrates that masticatory apparatus with an artificial oral environment is of interest in the fields including (i) dental science; (ii) food science; (iii) the pharmaceutical industries for drug release. However, apparatus that closely mimics human chewing and oral conditions has yet to be realised. This study investigates the vital role of dental morphology and form–function connections using two-bite test parameters for effective drug release from medicated chewing gum (MCG) and compares them to human chewing efficiency with the aid of a humanoid chewing robot and a bionics product lifecycle management (PLM) framework with built-in reverse biomimetics—both developed by the first author. Methods: A novel, bio-engineered two-bite testbed is created for two testing machines with compression and torsion capabilities to conduct two-bite tests for evaluating the mechanical properties of MCGs. Results: Experimental studies are conducted to investigate the relationship between biting force and crushing/shearing and understand chewing efficiency and effective mastication. This is with respect to mechanochemistry and power stroke for disrupting mechanical bonds releasing the active pharmaceutical ingredients (APIs) of MCGs. The manuscript discusses the effect and the critical role that jaw physiology, dental morphology, the Bennett angle of mandible (BA) and the Frankfort-mandibular plane angle (FMA) on two-bite test parameters when FMA = 0, 25 or 29.1 and BA = 0 or 8. Conclusions: The impact on other scientific fields is also explored. Full article
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19 pages, 7328 KiB  
Article
Experimental Raw Earth Building for Passive Cooling: A Case Study for Agricultural Application in a Mediterranean Climate
by Antoine Grosjean, Karim Touati, Gaël Alonzo, Homan Cheikh Ravat, Thomas Houot, Yassine El Mendili, Brigitte Nougarèdes and Nicolas Camara
Buildings 2025, 15(15), 2603; https://doi.org/10.3390/buildings15152603 - 23 Jul 2025
Viewed by 279
Abstract
Residential and agricultural buildings must prioritize environmental sustainability, employing locally sourced, bio/geologically sustainable materials, and reversible construction methods. Hence, adobe construction and earth-based building methods are experiencing a comeback. This article describes the hygrothermal performances of a real scale agricultural building prototype, in [...] Read more.
Residential and agricultural buildings must prioritize environmental sustainability, employing locally sourced, bio/geologically sustainable materials, and reversible construction methods. Hence, adobe construction and earth-based building methods are experiencing a comeback. This article describes the hygrothermal performances of a real scale agricultural building prototype, in real field conditions, built and designed to be energy-efficient, environmentally friendly, and well-suited for the hot, dry climates typical of the Mediterranean region during summer. The building prototype is a small modular two room construction, one room based on wood (for control purpose) and the other one on raw earth. The experimental set up highlights the passive cooling and humidity regulation potential provided by raw earth and adobe brick technology in agricultural buildings used for fruit and vegetable storage. Such passive cooling alternatives in the Mediterranean climate could reduce the need for energy-intensive and environmentally impactful cold storage rooms. Full article
(This article belongs to the Section Building Energy, Physics, Environment, and Systems)
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22 pages, 4544 KiB  
Article
Aspirin Eugenol Ester Ameliorates HFD-Induced NAFLD in Mice via the Modulation of Bile Acid Metabolism
by Zhi-Jie Zhang, Qi Tao, Ji Feng, Qin-Fang Yu, Li-Ping Fan, Zi-Hao Wang, Wen-Bo Ge, Jian-Yong Li and Ya-Jun Yang
Int. J. Mol. Sci. 2025, 26(15), 7044; https://doi.org/10.3390/ijms26157044 - 22 Jul 2025
Viewed by 160
Abstract
Non-alcoholic fatty liver disease (NAFLD) is a highly prevalent condition worldwide and represents a major global health challenge. Pharmacological and pharmacodynamic results indicate that aspirin eugenol ester (AEE) performs various pharmacological activities. However, it is unclear whether AEE can ameliorate the NAFLD. This [...] Read more.
Non-alcoholic fatty liver disease (NAFLD) is a highly prevalent condition worldwide and represents a major global health challenge. Pharmacological and pharmacodynamic results indicate that aspirin eugenol ester (AEE) performs various pharmacological activities. However, it is unclear whether AEE can ameliorate the NAFLD. This study investigated the ameliorative effects of AEE on glucose and lipid metabolism disorders by in vitro and in vivo experiments. In the cellular model, TC increased to 0.104 μmol/mg and TG increased to 0.152 μmol/mg in the model group, while TC decreased to 0.043 μmol/mg and TG decreased to 0.058 μmol/mg in the AEE group. In the model group, the area occupied by lipid droplets within the visual field was significantly elevated to 17.338%. However, the administration of AEE resulted in a substantial reduction in this area to 10.064%. AEE significantly reduced the lipid droplet area and TC and TG levels (p < 0.05), increased bile acids in the cells and in the medium supernatant (p < 0.05), and significantly up-regulated the expression of LRH-1, PPARα, CYP7A1, and BSEP mRNA levels (p < 0.05) compared to the model group. In the animal model, different doses of AEE administration significantly down-regulated the levels of TC, TG, LDL, GSP, and FBG (p < 0.05) compared to the high-fat-diet (HFD) group, and 216 mg/kg of AEE significantly improved hepatocellular steatosis, attenuated liver injury, and reduced the area of glycogen staining (p < 0.05). In the HFD group, the glycogen area within the visual field exhibited a significant increase to 18.250%. However, the administration of AEE resulted in a notable reduction in the glycogen area to 13.314%. Liver and serum metabolomics results show that AEE can reverse the metabolite changes caused by a HFD. The major metabolites were involved in seven pathways, including riboflavin metabolism, glycerophospholipid metabolism, tryptophan metabolism, primary bile acid biosynthesis, biosynthesis of unsaturated fatty acids, nicotinate and nicotinamide metabolism, and tryptophan metabolism. In conclusion, AEE had a positive regulatory effect on NAFLD. Full article
(This article belongs to the Special Issue Using Model Organisms to Study Complex Human Diseases)
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25 pages, 27161 KiB  
Article
Reverse-Engineering of the Japanese Defense Tactics During 1941–1945 Occupation Period in Hong Kong Through 21st-Century Geospatial Technologies
by Chun-Hei Lam, Chun-Ho Pun, Wallace-Wai-Lok Lai, Chi-Man Kwong and Craig Mitchell
Heritage 2025, 8(8), 294; https://doi.org/10.3390/heritage8080294 - 22 Jul 2025
Viewed by 230
Abstract
Hundreds of Japanese features of war (field positions, tunnels, and fortifications) were constructed in Hong Kong during World War II. However, most of them were poorly documented and were left unknown but still in relatively good condition because of their durable design, workmanship, [...] Read more.
Hundreds of Japanese features of war (field positions, tunnels, and fortifications) were constructed in Hong Kong during World War II. However, most of them were poorly documented and were left unknown but still in relatively good condition because of their durable design, workmanship, and remoteness. These features of war form parts of Hong Kong’s brutal history. Conservation, at least in digital form, is worth considering. With the authors coming from multidisciplinary and varied backgrounds, this paper aims to explore these features using a scientific workflow. First, we reviewed the surviving archival sources of the Imperial Japanese Army and Navy. Second, airborne LiDAR data were used to form territory digital terrain models (DTM) based on the Red Relief Image Map (RRIM) for identifying suspected locations. Third, field expeditions of searching for features of war were conducted through guidance of Global Navigation Satellite System—Real-Time Kinetics (GNSS-RTK). Fourth, the found features were 3D-laser scanned to generate mesh models as a digital archive and validate the findings of DTM-RRIM. This study represents a reverse-engineering effort to reconstruct the planned Japanese defense tactics of guerilla fight and Kamikaze grottos that were never used in Hong Kong. Full article
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32 pages, 3675 KiB  
Article
Gibbs Quantum Fields Computed by Action Mechanics Recycle Emissions Absorbed by Greenhouse Gases, Optimising the Elevation of the Troposphere and Surface Temperature Using the Virial Theorem
by Ivan R. Kennedy, Migdat Hodzic and Angus N. Crossan
Thermo 2025, 5(3), 25; https://doi.org/10.3390/thermo5030025 - 22 Jul 2025
Viewed by 202
Abstract
Atmospheric climate science lacks the capacity to integrate thermodynamics with the gravitational potential of air in a classical quantum theory. To what extent can we identify Carnot’s ideal heat engine cycle in reversible isothermal and isentropic phases between dual temperatures partitioning heat flow [...] Read more.
Atmospheric climate science lacks the capacity to integrate thermodynamics with the gravitational potential of air in a classical quantum theory. To what extent can we identify Carnot’s ideal heat engine cycle in reversible isothermal and isentropic phases between dual temperatures partitioning heat flow with coupled work processes in the atmosphere? Using statistical action mechanics to describe Carnot’s cycle, the maximum rate of work possible can be integrated for the working gases as equal to variations in the absolute Gibbs energy, estimated as sustaining field quanta consistent with Carnot’s definition of heat as caloric. His treatise of 1824 even gave equations expressing work potential as a function of differences in temperature and the logarithm of the change in density and volume. Second, Carnot’s mechanical principle of cooling caused by gas dilation or warming by compression can be applied to tropospheric heat–work cycles in anticyclones and cyclones. Third, the virial theorem of Lagrange and Clausius based on least action predicts a more accurate temperature gradient with altitude near 6.5–6.9 °C per km, requiring that the Gibbs rotational quantum energies of gas molecules exchange reversibly with gravitational potential. This predicts a diminished role for the radiative transfer of energy from the atmosphere to the surface, in contrast to the Trenberth global radiative budget of ≈330 watts per square metre as downwelling radiation. The spectral absorptivity of greenhouse gas for surface radiation into the troposphere enables thermal recycling, sustaining air masses in Lagrangian action. This obviates the current paradigm of cooling with altitude by adiabatic expansion. The virial-action theorem must also control non-reversible heat–work Carnot cycles, with turbulent friction raising the surface temperature. Dissipative surface warming raises the surface pressure by heating, sustaining the weight of the atmosphere to varying altitudes according to latitude and seasonal angles of insolation. New predictions for experimental testing are now emerging from this virial-action hypothesis for climate, linking vortical energy potential with convective and turbulent exchanges of work and heat, proposed as the efficient cause setting the thermal temperature of surface materials. Full article
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27 pages, 6704 KiB  
Article
Dynamic Characteristics of a Digital Hydraulic Drive System for an Emergency Drainage Pump Under Alternating Loads
by Yong Zhu, Yinghao Liu, Qingyi Wu and Qiang Gao
Machines 2025, 13(8), 636; https://doi.org/10.3390/machines13080636 - 22 Jul 2025
Viewed by 211
Abstract
With the frequent occurrence of global floods, the demand for emergency rescue equipment has grown rapidly. The development and technological innovation of digital hydraulic drive systems (DHDSs) for emergency drainage pumps (EDPs) have become key to improving rescue efficiency. However, EDPs are prone [...] Read more.
With the frequent occurrence of global floods, the demand for emergency rescue equipment has grown rapidly. The development and technological innovation of digital hydraulic drive systems (DHDSs) for emergency drainage pumps (EDPs) have become key to improving rescue efficiency. However, EDPs are prone to being affected by random and uncertain loads during operation. To achieve intelligent and efficient rescue operations, a DHDS suitable for EDPs was proposed. Firstly, the configuration and operation mode of the DHDS for EDPs were analyzed. Based on this, a multi-field coupling dynamic simulation platform for the DHDS was constructed. Secondly, the output characteristics of the system under alternating loads were simulated and analyzed. Finally, a test platform for the EDP DHDS was established, and the dynamic characteristics of the system under alternating loads were explored. The results show that as the load torque of the alternating loads increases, the amplitude of the pressure of the motor also increases, the output flow of the hydraulic-controlled proportional reversing valve (HCPRV) changes slightly, and the fluctuation range of the rotational speed of the motor increases. The fluctuation range of the pressure and the rotational speed of the motor are basically not affected by the frequency of alternating loads, but the fluctuation amplitude of the output flow of the HCPRV reduces with the increase in the frequency of alternating loads. This system can respond to changes in load relatively quickly under alternating loads and can return to a stable state in a short time. It has laudable anti-interference ability and output stability. Full article
(This article belongs to the Section Electrical Machines and Drives)
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18 pages, 5521 KiB  
Article
Design and TCAD Simulation of GaN P-i-N Diode with Multi-Drift-Layer and Field-Plate Termination Structures
by Zhibo Yang, Guanyu Wang, Yifei Wang, Pandi Mao and Bo Ye
Micromachines 2025, 16(8), 839; https://doi.org/10.3390/mi16080839 - 22 Jul 2025
Viewed by 277
Abstract
Vertical GaN P-i-N diodes exhibit excellent high-voltage performance, fast switching speed, and low conduction losses, making them highly attractive for power applications. However, their breakdown voltage is severely constrained by electric field crowding at device edges. Using silvaco tcad (2019) tools, this work [...] Read more.
Vertical GaN P-i-N diodes exhibit excellent high-voltage performance, fast switching speed, and low conduction losses, making them highly attractive for power applications. However, their breakdown voltage is severely constrained by electric field crowding at device edges. Using silvaco tcad (2019) tools, this work systematically evaluates multiple edge termination techniques, including deep-etched mesa, beveled mesa, and field-plate configurations with both vertical and inclined mesa structures. We present an optimized multi-drift-layer GaN P-i-N diode incorporating field-plate termination and analyze its electrical performance in detail. This study covers forward conduction characteristics including on-state voltage, on-resistance, and their temperature dependence, reverse breakdown behavior examining voltage capability and electric field distribution under different temperatures, and switching performance addressing both forward recovery phenomena, i.e., voltage overshoot and carrier injection dynamics, and reverse recovery characteristics including peak current and recovery time. The comprehensive analysis offers practical design guidelines for developing high-performance GaN power devices. Full article
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10 pages, 1491 KiB  
Article
Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses
by Jianjun Jia, Wenjun Zhu, Guodong Liu, Sandra Diederich, Bradley Pickering, Logan Banadyga and Ming Yang
Viruses 2025, 17(7), 1021; https://doi.org/10.3390/v17071021 - 21 Jul 2025
Viewed by 301
Abstract
Nipah virus (NiV) and Hendra virus (HeV), which both belong to the genus henipavirus, are zoonotic pathogens that cause severe systemic, neurological, and/or respiratory disease in humans and a variety of mammals. Therefore, monitoring viral prevalence in natural reservoirs and rapidly diagnosing cases [...] Read more.
Nipah virus (NiV) and Hendra virus (HeV), which both belong to the genus henipavirus, are zoonotic pathogens that cause severe systemic, neurological, and/or respiratory disease in humans and a variety of mammals. Therefore, monitoring viral prevalence in natural reservoirs and rapidly diagnosing cases of henipavirus infection are critical to limiting the spread of these viruses. Current laboratory methods for detecting NiV and HeV include virus isolation, reverse transcription quantitative real-time PCR (RT-qPCR), and antigen detection via an enzyme-linked immunosorbent assay (ELISA), all of which require highly trained personnel and specialized equipment. Here, we describe the development of a point-of-care customized immunochromatographic lateral flow (ILF) assay that uses recombinant human ephrin B2 as a capture ligand on the test line and a NiV-specific monoclonal antibody (mAb) on the conjugate pad to detect NiV and HeV. The ILF assay detects NiV and HeV with a diagnostic specificity of 94.4% and has no cross-reactivity with other viruses. This rapid test may be suitable for field testing and in countries with limited laboratory resources. Full article
(This article belongs to the Section General Virology)
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13 pages, 3976 KiB  
Article
Streamlining First-Order Reversal Curves Analysis of Molecular Magnetism Bistability Using a Calorimetric Approach
by Diana Plesca, Cristian Enachescu, Radu Tanasa, Alexandru Stancu, Denis Morineau and Marie-Laure Boillot
Materials 2025, 18(14), 3413; https://doi.org/10.3390/ma18143413 - 21 Jul 2025
Viewed by 226
Abstract
We present an alternative to the classical SQUID magnetometric measurements for the First-Order Reversal Curve (FORC) diagram approach by employing differential scanning calorimetry (DSC) experiments. After discussing the main results, the advantages and limitations of the magnetometric FORCs, we introduce the calorimetric method. [...] Read more.
We present an alternative to the classical SQUID magnetometric measurements for the First-Order Reversal Curve (FORC) diagram approach by employing differential scanning calorimetry (DSC) experiments. After discussing the main results, the advantages and limitations of the magnetometric FORCs, we introduce the calorimetric method. We argue that, while the results are comparable to those obtained via magnetometry, the calorimetric method not only significantly simplifies the required mathematical computations but also detects subtle or overlapping phase transitions that might be hard to distinguish magnetically. The methodology is illustrated through both experimental data and mean-field simulations. Full article
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17 pages, 2091 KiB  
Article
A Novel Parvovirus Associated with the Whitefly Bemisia tabaci
by Fani Gousi, Zineb Belabess, Nathalie Laboureau, Michel Peterschmitt and Mikhail M. Pooggin
Pathogens 2025, 14(7), 714; https://doi.org/10.3390/pathogens14070714 - 19 Jul 2025
Viewed by 319
Abstract
The whitefly Bemisia tabaci (Hemiptera: Aleyrodoidea) causes direct feeding damage to crop plants and transmits pathogenic plant viruses, thereby threatening global food security. Although whitefly-infecting RNA viruses are known and proposed as biocontrol agents, no insect DNA virus has been found in any [...] Read more.
The whitefly Bemisia tabaci (Hemiptera: Aleyrodoidea) causes direct feeding damage to crop plants and transmits pathogenic plant viruses, thereby threatening global food security. Although whitefly-infecting RNA viruses are known and proposed as biocontrol agents, no insect DNA virus has been found in any member of Aleyrodoidea. Using rolling circle amplification (RCA) of viral DNA from whiteflies collected from crop fields in Morocco, followed by Illumina sequencing of the RCA products, we found a novel insect single-stranded (ss) DNA parvovirus (family Parvoviridae) in addition to plant ssDNA geminiviruses transmitted by whiteflies. Based on its genome organization with inverted terminal repeats and evolutionarily conserved proteins mediating viral DNA replication (NS1/Rep) and encapsidation (VP), encoded on the forward and reverse strands, respectively, we named this virus Bemisia tabaci ambidensovirus (BtaDV) and classified it as a founding member of a new genus within the subfamily Densovirinae. This subfamily also contains three distinct genera of ambisense densoviruses of other hemipteran insects (Aphidoidea, Coccoidea, and Psylloidea). Furthermore, we provide evidence for the genetic variants of BtaDV circulating in whitefly populations and for its partial sequences integrated into the B. tabaci genome, with one integrant locus potentially expressing a fusion protein composed of viral Rep endonuclease and host DNA-binding domains. This suggests a long-term virus-host interaction and neofunctionalization of BtaDV-derived endogenous viral elements. Full article
(This article belongs to the Section Viral Pathogens)
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