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22 pages, 20111 KiB  
Article
Streamflow Forecasting: A Comparative Analysis of ARIMAX, Rolling Forecasting LSTM Neural Network and Physically Based Models in a Pristine Catchment
by Diego Perazzolo, Gianluca Lazzaro, Alvise Fiume, Pietro Fanton and Enrico Grisan
Water 2025, 17(15), 2341; https://doi.org/10.3390/w17152341 - 6 Aug 2025
Abstract
Accurate streamflow forecasting at fine temporal and spatial scales is essential to manage the diverse hydrological behaviors of individual catchments, particularly in rapidly responding mountainous regions. This study compares three forecasting models ARIMAX, LSTM, and HEC-HMS applied to the Posina River basin in [...] Read more.
Accurate streamflow forecasting at fine temporal and spatial scales is essential to manage the diverse hydrological behaviors of individual catchments, particularly in rapidly responding mountainous regions. This study compares three forecasting models ARIMAX, LSTM, and HEC-HMS applied to the Posina River basin in northern Italy, using 13 years of hourly hydrological data. While recent literature promotes multi-basin LSTM training for generalization, we show that a well-configured single-basin LSTM, combined with a rolling forecast strategy, can achieve comparable accuracy under high-frequency, data-constrained conditions. The physically based HEC-HMS model, calibrated for continuous simulation, provides robust peak flow prediction but requires extensive parameter tuning. ARIMAX captures baseflows but underestimates sharp hydrological events. Evaluation through NSE, KGE, and MAE shows that both LSTM and HEC-HMS outperform ARIMAX, with LSTM offering a compelling balance between accuracy and ease of implementation. This study enhances our understanding of streamflow model behavior in small basins and demonstrates that LSTM networks, despite their simplified configuration, can be reliable tools for flood forecasting in localized Alpine catchments, where physical modeling is resource-intensive and regional data for multi-basin training are often unavailable. Full article
20 pages, 8429 KiB  
Article
Altitude and Temperature Drive Spatial and Temporal Changes in Vegetation Cover on the Eastern Tibetan Plateau
by Yu Feng, Hongjin Zhu, Xiaojuan Zhang, Feilong Qin, Peng Ye, Pengtao Niu, Xueman Wang and Songlin Shi
Earth 2025, 6(3), 92; https://doi.org/10.3390/earth6030092 (registering DOI) - 6 Aug 2025
Abstract
The Tibetan Plateau (TP) is experiencing higher warming rates than elsewhere, which may affect regional vegetation growth. Particularly on the Eastern Tibetan Plateau (ETP), where the topography is diverse and rich in biodiversity, it is necessary to clarify the drivers of climate and [...] Read more.
The Tibetan Plateau (TP) is experiencing higher warming rates than elsewhere, which may affect regional vegetation growth. Particularly on the Eastern Tibetan Plateau (ETP), where the topography is diverse and rich in biodiversity, it is necessary to clarify the drivers of climate and topography on vegetation cover. In this research, we selected the Shaluli Mountains (SLLM) in the ETP as the study area, monitored the spatial and temporal dynamics of the regional vegetation cover using remote sensing methods, and quantified the drivers of vegetation change using Geodetector (GD). The results showed a decreasing trend in annual precipitation (PRE) (−2.4054 mm/year) and the Palmer Drought Severity Index (PDSI) (−0.1813/year) in the SLLM. Annual maximum temperature (TMX) on the spatial and temporal scales showed an overall increasing trend, and the regional climate tended to become warmer and drier. Since 2000, fractional vegetation cover (FVC) has shown a fluctuating upward trend, with an average value of 0.6710, and FVC has spatially shown a pattern of “low in the middle and high in the surroundings”. The areas with non-significant increases (p > 0.05) and significant increases (p < 0.05) in FVC accounted for 46.03% and 5.76% of the SLLM. Altitude (q = 0.3517) and TMX (q = 0.3158) were the main drivers of FVC changes. As altitude and TMX increased, FVC showed a trend of increasing and then decreasing. The results of this study help us to clarify the influence of climate and topography on the vegetation ecosystem of the ETP and provide a scientific basis for regional biodiversity conservation and sustainable development. Full article
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30 pages, 16226 KiB  
Article
A Dual-Stage and Dual-Population Algorithm Based on Chemical Reaction Optimization for Constrained Multi-Objective Optimization
by Tianyu Zhang, Xin Guo, Yan Li, Na Li, Ruochen Zheng, Wenbo Dong and Weichao Ding
Processes 2025, 13(8), 2484; https://doi.org/10.3390/pr13082484 - 6 Aug 2025
Abstract
Constrained multi-objective optimization problems (CMOPs) require optimizing multiple conflicting objectives while satisfying complex constraints. These constraints generate infeasible regions that challenge traditional algorithms in balancing feasibility and Pareto frontier diversity. chemical reaction optimization (CRO) effectively balances global exploration and local exploitation through molecular [...] Read more.
Constrained multi-objective optimization problems (CMOPs) require optimizing multiple conflicting objectives while satisfying complex constraints. These constraints generate infeasible regions that challenge traditional algorithms in balancing feasibility and Pareto frontier diversity. chemical reaction optimization (CRO) effectively balances global exploration and local exploitation through molecular collision reactions and energy management, thereby enhancing search efficiency. However, standard CRO variants often struggle with CMOPs due to the absence of specialized constraint-handling mechanisms. To address these challenges, this paper integrates the CRO collision reaction mechanism with an existing evolutionary computational framework to design a dual-stage and dual-population chemical reaction optimization (DDCRO) algorithm. This approach employs a staged optimization strategy, which divides population evolution into two phases. The first phase focuses on objective optimization to enhance population diversity, and the second prioritizes constraint satisfaction to accelerate convergence toward the constrained Pareto front. Furthermore, to leverage the infeasible solutions’ guiding potential during the search, DDCRO adopts a two-population strategy. At each stage, the main population tackles the original constrained problem, while the auxiliary population addresses the corresponding unconstrained version. A weak complementary mechanism facilitates information sharing between populations, which enhances search efficiency and algorithmic robustness. Comparative tests on multiple test suites reveal that DDCRO achieves optimal IGD/HV values in 53% of test problems. The proposed algorithm outperforms other state-of-the-art algorithms in both convergence and population diversity. Full article
(This article belongs to the Section Chemical Processes and Systems)
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16 pages, 22496 KiB  
Article
Comparative Genomics and Adaptive Evolution of Bifidobacterium adolescentis in Geographically Distinct Human Gut Populations
by Pei Fu, Hao Qi and Wenjun Liu
Foods 2025, 14(15), 2747; https://doi.org/10.3390/foods14152747 - 6 Aug 2025
Abstract
Bifidobacterium adolescentis is prevalent in the gastrointestinal tract of healthy humans, and significantly influences host health. Recent studies have predominantly investigated the probiotic characteristics of individual strains and their specific metabolic roles, whereas analyses at the population genome level have been limited to [...] Read more.
Bifidobacterium adolescentis is prevalent in the gastrointestinal tract of healthy humans, and significantly influences host health. Recent studies have predominantly investigated the probiotic characteristics of individual strains and their specific metabolic roles, whereas analyses at the population genome level have been limited to date. This study conducted a comparative genomics analysis of 543 B. adolescentis genomes to explore genetic background variations and functional gene differences across geographically diverse populations. The results revealed significant differences in genome size and GC content among populations from Asia, Europe, and North America (p < 0.05). The pan-gene exhibited an open structure, reflecting the substantial genetic diversity within B. adolescentis. Functional annotation demonstrated that B. adolescentis possesses numerous protein-coding genes and abundant carbohydrate-active enzymes (CAZys) implicated in carbohydrate degradation and transformation. Population-specific CAZys were identified, suggesting adaptive evolution driven by distinct regional dietary patterns. Full article
(This article belongs to the Section Food Microbiology)
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22 pages, 6201 KiB  
Article
SOAM Block: A Scale–Orientation-Aware Module for Efficient Object Detection in Remote Sensing Imagery
by Yi Chen, Zhidong Wang, Zhipeng Xiong, Yufeng Zhang and Xinqi Xu
Symmetry 2025, 17(8), 1251; https://doi.org/10.3390/sym17081251 - 6 Aug 2025
Abstract
Object detection in remote sensing imagery is critical in environmental monitoring, urban planning, and land resource management. However, the task remains challenging due to significant scale variations, arbitrary object orientations, and complex background clutter. To address these issues, we propose a novel orientation [...] Read more.
Object detection in remote sensing imagery is critical in environmental monitoring, urban planning, and land resource management. However, the task remains challenging due to significant scale variations, arbitrary object orientations, and complex background clutter. To address these issues, we propose a novel orientation module (SOAM Block) that jointly models object scale and directional features while exploiting geometric symmetry inherent in many remote sensing targets. The SOAM Block is constructed upon a lightweight and efficient Adaptive Multi-Scale (AMS) Module, which utilizes a symmetric arrangement of parallel depth-wise convolutional branches with varied kernel sizes to extract fine-grained multi-scale features without dilation, thereby preserving local context and enhancing scale adaptability. In addition, a Strip-based Context Attention (SCA) mechanism is introduced to model long-range spatial dependencies, leveraging horizontal and vertical 1D strip convolutions in a directionally symmetric fashion. This design captures spatial correlations between distant regions and reinforces semantic consistency in cluttered scenes. Importantly, this work is the first to explicitly analyze the coupling between object scale and orientation in remote sensing imagery. The proposed method addresses the limitations of fixed receptive fields in capturing symmetric directional cues of large-scale objects. Extensive experiments are conducted on two widely used benchmarks—DOTA and HRSC2016—both of which exhibit significant scale variations and orientation diversity. Results demonstrate that our approach achieves superior detection accuracy with fewer parameters and lower computational overhead compared to state-of-the-art methods. The proposed SOAM Block thus offers a robust, scalable, and symmetry-aware solution for high-precision object detection in complex aerial scenes. Full article
(This article belongs to the Section Computer)
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21 pages, 49475 KiB  
Article
NRGS-Net: A Lightweight Uformer with Gated Positional and Local Context Attention for Nighttime Road Glare Suppression
by Ruoyu Yang, Huaixin Chen, Sijie Luo and Zhixi Wang
Appl. Sci. 2025, 15(15), 8686; https://doi.org/10.3390/app15158686 (registering DOI) - 6 Aug 2025
Abstract
Existing nighttime visibility enhancement methods primarily focus on improving overall brightness under low-light conditions. However, nighttime road images are also affected by glare, glow, and flare from complex light sources such as streetlights and headlights, making it challenging to suppress locally overexposed regions [...] Read more.
Existing nighttime visibility enhancement methods primarily focus on improving overall brightness under low-light conditions. However, nighttime road images are also affected by glare, glow, and flare from complex light sources such as streetlights and headlights, making it challenging to suppress locally overexposed regions and recover fine details. To address these challenges, we propose a Nighttime Road Glare Suppression Network (NRGS-Net) for glare removal and detail restoration. Specifically, to handle diverse glare disturbances caused by the uncertainty in light source positions and shapes, we designed a gated positional attention (GPA) module that integrates positional encoding with local contextual information to guide the network in accurately locating and suppressing glare regions, thereby enhancing the visibility of affected areas. Furthermore, we introduced an improved Uformer backbone named LCAtransformer, in which the downsampling layers adopt efficient depthwise separable convolutions to reduce computational cost while preserving critical spatial information. The upsampling layers incorporate a residual PixelShuffle module to achieve effective restoration in glare-affected regions. Additionally, channel attention is introduced within the Local Context-Aware Feed-Forward Network (LCA-FFN) to enable adaptive adjustment of feature weights, effectively suppressing irrelevant and interfering features. To advance the research in nighttime glare suppression, we constructed and publicly released the Night Road Glare Dataset (NRGD) captured in real nighttime road scenarios, enriching the evaluation system for this task. Experiments conducted on the Flare7K++ and NRGD, using five evaluation metrics and comparing six state-of-the-art methods, demonstrate that our method achieves superior performance in both subjective and objective metrics compared to existing advanced methods. Full article
(This article belongs to the Special Issue Computational Imaging: Algorithms, Technologies, and Applications)
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17 pages, 326 KiB  
Article
Remittances and FDI: Drivers of Employment in the Economic Community of West African States
by Grace Toyin Adigun, Abiola John Asaleye, Olayinka Omolara Adenikinju, Kehinde Damilola Ilesanmi, Sunday Festus Olasupo and Adedoyin Isola Lawal
J. Risk Financial Manag. 2025, 18(8), 436; https://doi.org/10.3390/jrfm18080436 - 6 Aug 2025
Abstract
Unemployment and weak economic productivity are significant global issues, particularly in West Africa. Recently, through diverse mechanisms, remittances and foreign direct investment (FDI) have been sources of foreign capital flow that have positively influenced many less developed economies, including ECOWAS (ECOWAS stands for [...] Read more.
Unemployment and weak economic productivity are significant global issues, particularly in West Africa. Recently, through diverse mechanisms, remittances and foreign direct investment (FDI) have been sources of foreign capital flow that have positively influenced many less developed economies, including ECOWAS (ECOWAS stands for Economic Community of West African States). Nevertheless, these financial flows have exhibited significant inconsistencies, primarily resulting from economic downturns in migrants’ destination countries, with remarkable implications for beneficiary economies. This study, therefore, examines the effect of remittances and FDI on employment in ECOWAS. Specifically, the study assesses the effects of the inflow of remittances and FDI on employment using panel dynamic ordinary least squares (PDOLS) and also investigates the shock effects of remittances and FDI by employing Panel Vector Error Correction (PVECM), which involves variance decomposition. The results show that foreign direct investment (FDI) positively and significantly affects employment. Other variables that show a significant relationship with employment are wage rate, education expenditure, and interest rate. The variance decomposition result revealed that external shocks on remittances and FDI have short- and long-term effects on employment. The above findings imply that foreign direct investment has a far-reaching positive impact on the economy-wide management of the West African sub-region and thus calls for relevant policy options. Full article
(This article belongs to the Special Issue Macroeconomic Dynamics and Economic Growth)
23 pages, 2394 KiB  
Article
Functional, Antioxidant, and Antimicrobial Profile of Medicinal Leaves from the Amazon
by Gabriela Méndez, Elena Coyago-Cruz, Paola Lomas, Marco Cerna and Jorge Heredia-Moya
Antioxidants 2025, 14(8), 965; https://doi.org/10.3390/antiox14080965 (registering DOI) - 5 Aug 2025
Abstract
The Amazon region is home to a remarkable diversity of plant species that are used in traditional medicine and cuisine. This study aimed to evaluate the functional, antioxidant, and antimicrobial properties of the leaves of Allium schoenoprasum, Brugmansia candida (white and pink), [...] Read more.
The Amazon region is home to a remarkable diversity of plant species that are used in traditional medicine and cuisine. This study aimed to evaluate the functional, antioxidant, and antimicrobial properties of the leaves of Allium schoenoprasum, Brugmansia candida (white and pink), and Cyclanthemum bipartitum. Bioactive compounds (L-ascorbic acid, organic acids, carotenoids, phenolic compounds, and chlorophylls) were quantified using liquid chromatography. The ABTS and DPPH methods were used to assess the antioxidant capacity. Additionally, the antimicrobial activity against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus mutans, Candida albicans, and Candida tropicalis was evaluated. The results revealed a high content of L-ascorbic acid (7.6 mg/100 g dry weight) and total carotenoids (509.0 mg/100 g dry weight), as well as high antioxidant capacity (4.5 mmol TE/100 g dry weight) and broad antimicrobial activity in Brugmansia candida ‘pink’. The White variety had the highest concentration of total chlorophylls (1742.8 mg/100 g DW), Cyclanthemum bipartitum had the highest total organic acid content (2814.5 mg/100 g DW), and Allium schoenoprasum had the highest concentration of total phenolic compounds (11,351.6 mg/100 g DW). These results constitute a starting point for future research, emphasizing the potential health risks that certain species may pose. Full article
(This article belongs to the Special Issue Plant Materials and Their Antioxidant Potential, 2nd Edition)
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23 pages, 85184 KiB  
Article
MB-MSTFNet: A Multi-Band Spatio-Temporal Attention Network for EEG Sensor-Based Emotion Recognition
by Cheng Fang, Sitong Liu and Bing Gao
Sensors 2025, 25(15), 4819; https://doi.org/10.3390/s25154819 - 5 Aug 2025
Abstract
Emotion analysis based on electroencephalogram (EEG) sensors is pivotal for human–machine interaction yet faces key challenges in spatio-temporal feature fusion and cross-band and brain-region integration from multi-channel sensor-derived signals. This paper proposes MB-MSTFNet, a novel framework for EEG emotion recognition. The model constructs [...] Read more.
Emotion analysis based on electroencephalogram (EEG) sensors is pivotal for human–machine interaction yet faces key challenges in spatio-temporal feature fusion and cross-band and brain-region integration from multi-channel sensor-derived signals. This paper proposes MB-MSTFNet, a novel framework for EEG emotion recognition. The model constructs a 3D tensor to encode band–space–time correlations of sensor data, explicitly modeling frequency-domain dynamics and spatial distributions of EEG sensors across brain regions. A multi-scale CNN-Inception module extracts hierarchical spatial features via diverse convolutional kernels and pooling operations, capturing localized sensor activations and global brain network interactions. Bi-directional GRUs (BiGRUs) model temporal dependencies in sensor time-series, adept at capturing long-range dynamic patterns. Multi-head self-attention highlights critical time windows and brain regions by assigning adaptive weights to relevant sensor channels, suppressing noise from non-contributory electrodes. Experiments on the DEAP dataset, containing multi-channel EEG sensor recordings, show that MB-MSTFNet achieves 96.80 ± 0.92% valence accuracy, 98.02 ± 0.76% arousal accuracy for binary classification tasks, and 92.85 ± 1.45% accuracy for four-class classification. Ablation studies validate that feature fusion, bidirectional temporal modeling, and multi-scale mechanisms significantly enhance performance by improving feature complementarity. This sensor-driven framework advances affective computing by integrating spatio-temporal dynamics and multi-band interactions of EEG sensor signals, enabling efficient real-time emotion recognition. Full article
(This article belongs to the Section Intelligent Sensors)
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27 pages, 14923 KiB  
Article
Multi-Sensor Flood Mapping in Urban and Agricultural Landscapes of the Netherlands Using SAR and Optical Data with Random Forest Classifier
by Omer Gokberk Narin, Aliihsan Sekertekin, Caglar Bayik, Filiz Bektas Balcik, Mahmut Arıkan, Fusun Balik Sanli and Saygin Abdikan
Remote Sens. 2025, 17(15), 2712; https://doi.org/10.3390/rs17152712 - 5 Aug 2025
Abstract
Floods stand as one of the most harmful natural disasters, which have become more dangerous because of climate change effects on urban structures and agricultural fields. This research presents a comprehensive flood mapping approach that combines multi-sensor satellite data with a machine learning [...] Read more.
Floods stand as one of the most harmful natural disasters, which have become more dangerous because of climate change effects on urban structures and agricultural fields. This research presents a comprehensive flood mapping approach that combines multi-sensor satellite data with a machine learning method to evaluate the July 2021 flood in the Netherlands. The research developed 25 different feature scenarios through the combination of Sentinel-1, Landsat-8, and Radarsat-2 imagery data by using backscattering coefficients together with optical Normalized Difference Water Index (NDWI) and Hue, Saturation, and Value (HSV) images and Synthetic Aperture Radar (SAR)-derived Grey Level Co-occurrence Matrix (GLCM) texture features. The Random Forest (RF) classifier was optimized before its application based on two different flood-prone regions, which included Zutphen’s urban area and Heijen’s agricultural land. Results demonstrated that the multi-sensor fusion scenarios (S18, S20, and S25) achieved the highest classification performance, with overall accuracy reaching 96.4% (Kappa = 0.906–0.949) in Zutphen and 87.5% (Kappa = 0.754–0.833) in Heijen. For the flood class F1 scores of all scenarios, they varied from 0.742 to 0.969 in Zutphen and from 0.626 to 0.969 in Heijen. Eventually, the addition of SAR texture metrics enhanced flood boundary identification throughout both urban and agricultural settings. Radarsat-2 provided limited benefits to the overall results, since Sentinel-1 and Landsat-8 data proved more effective despite being freely available. This study demonstrates that using SAR and optical features together with texture information creates a powerful and expandable flood mapping system, and RF classification performs well in diverse landscape settings. Full article
(This article belongs to the Special Issue Remote Sensing Applications in Flood Forecasting and Monitoring)
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17 pages, 1388 KiB  
Article
Invertebrate Assemblages in Some Saline and Soda Lakes of the Kulunda Steppe: First Regional Assessment and Ecological Implications
by Larisa Golovatyuk, Timur Kanapatskiy, Olga Samylina, Nikolay Pimenov, Larisa Nazarova and Anna Kallistova
Water 2025, 17(15), 2330; https://doi.org/10.3390/w17152330 - 5 Aug 2025
Abstract
The taxonomic composition and structure of invertebrate assemblages in five lakes from the Kulunda steppe, located in an arid region of southwestern Siberia (Russia), were studied. The lakes varied greatly in their total salinity (5 to 304 g L−1) and carbonate [...] Read more.
The taxonomic composition and structure of invertebrate assemblages in five lakes from the Kulunda steppe, located in an arid region of southwestern Siberia (Russia), were studied. The lakes varied greatly in their total salinity (5 to 304 g L−1) and carbonate alkalinity (0.03 to 4.03 mol-eq L−1). The invertebrate fauna was characterized by low diversity. Only five taxa of macrozoobenthos and two taxa of planktonic invertebrates were identified. As water salinity increased, the taxonomic diversity of the studied lakes decreased, and at salinities > 276 g L−1, monodominant assemblages were formed. The high numbers and biomass of aquatic organism provide a rich food supply for native and migratory waterfowl. The low taxonomic diversity of the invertebrate assemblages of the lakes makes them vulnerable to any negative external impact. The climate in the Kulunda steppe demonstrates a long-term aridization trend. If this continues in the future, then over time, this may lead to the gradual salinization of lakes and a further decrease in the taxonomic diversity of hydrobiological assemblages. This emphasizes the ecological importance of the studied territory and the necessity for its inclusion in the list of sites protected by the Ramsar Convention. Full article
(This article belongs to the Section Biodiversity and Functionality of Aquatic Ecosystems)
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16 pages, 4074 KiB  
Article
Exploring 6-aza-2-Thiothymine as a MALDI-MSI Matrix for Spatial Lipidomics of Formalin-Fixed Paraffin-Embedded Clinical Samples
by Natalia Shelly Porto, Simone Serrao, Greta Bindi, Nicole Monza, Claudia Fumagalli, Vanna Denti, Isabella Piga and Andrew Smith
Metabolites 2025, 15(8), 531; https://doi.org/10.3390/metabo15080531 - 5 Aug 2025
Abstract
Background/Objectives: In recent years, lipids have emerged as critical regulators of different disease processes, being involved in cancer pathogenesis, progression, and outcome. Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI) has significantly expanded the technology’s reach, enabling spatially resolved profiling of lipids directly [...] Read more.
Background/Objectives: In recent years, lipids have emerged as critical regulators of different disease processes, being involved in cancer pathogenesis, progression, and outcome. Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI) has significantly expanded the technology’s reach, enabling spatially resolved profiling of lipids directly from tissue, including formalin-fixed paraffin-embedded (FFPE) specimens. In this context, MALDI matrix selection is crucial for lipid extraction and ionization, influencing key aspects such as molecular coverage and sensitivity, especially in such specimens with already depleted lipid content. Thus, in this work, we aim to explore the feasibility of mapping lipid species in FFPE clinical samples with MALDI-MSI using 6-aza-2-thiothymine (ATT) as a matrix of choice. Methods: To do so, ATT performances were first compared to those two other matrices commonly used for lipidomic analyses, 2′,5′-dihydroxybenzoic acid (DHB) and Norharmane (NOR), on lipid standards. Results: As a proof-of-concept, we then assessed ATT’s performance for the MALDI-MSI analysis of lipids in FFPE brain sections, both in positive and negative ion modes, comparing results with those obtained from other commonly used dual-polarity matrices. In this context, ATT enabled the putative annotation of 98 lipids while maintaining a well-balanced detection of glycerophospholipids (60.2%) and sphingolipids (32.7%) in positive ion mode. It outperformed both DHB and NOR in the identification of glycolipids (3%) and fatty acids (4%). Additionally, ATT exceeded DHB in terms of total lipid count (62 vs. 21) and class diversity and demonstrated performance comparable to NOR in negative ion mode. Moreover, ATT was applied to a FFPE glioblastoma tissue microarray (TMA) evaluating the ability of this matrix to reveal biologically relevant lipid features capable of distinguishing normal brain tissue from glioblastoma regions. Conclusions: Altogether, the results presented in this work suggest that ATT is a suitable matrix for pathology imaging applications, even at higher lateral resolutions of 20 μm, not only for proteomic but also for lipidomic analysis. This could enable the use of the same matrix type for the analysis of both lipids and peptides on the same tissue section, offering a unique strategic advantage for multi-omics studies, while also supporting acquisition in both positive and negative ionization modes. Full article
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18 pages, 3140 KiB  
Article
Spatial and Temporal Distribution of Conversational and Emerging Pollutants in Fecal Sludge from Rural Toilets, China
by Lin Lin, Yilin Shen, Guoji Ding, Shakib Alghashm, Seinn Lei Aye and Xiaowei Li
Sustainability 2025, 17(15), 7088; https://doi.org/10.3390/su17157088 - 5 Aug 2025
Abstract
Effective management of fecal pollutants in rural sanitation is crucial for environmental health and public safety, especially in developing regions. In this study, temporal and regional variations in nutrient elements, heavy metals, pathogenic microorganisms (PMs), and antibiotic resistance genes (ARGs) of fecal samples [...] Read more.
Effective management of fecal pollutants in rural sanitation is crucial for environmental health and public safety, especially in developing regions. In this study, temporal and regional variations in nutrient elements, heavy metals, pathogenic microorganisms (PMs), and antibiotic resistance genes (ARGs) of fecal samples from rural toilets in China were investigated. The moisture contents of the fecal samples average 92.7%, decreasing seasonally from 97.4% in summer to 90.6% in winter. The samples’ pH values range from 6.5 to 7.5, with a slight decrease in winter (6.8), while their electrical conductivity varies from 128.1 to 2150 μs/cm, influenced by regional diets. Chromium (9.0–49.7 mg/kg) and copper (31.9–784.4 mg/kg) levels vary regionally, with higher concentrations in Anhui and Guangxi Provinces due to dietary and industrial factors. Zinc contents range from 108.5 to 1648.9 mg/kg, with higher levels in autumn and winter, resulting from agricultural practices and Zn-containing fungicides, posing potential health and phytotoxicity risks. Seasonal and regional variations in PMs and ARGs were observed. Guangxi Province shows the high PM diversity in summer samples, while Jiangsu Province exhibits the high ARGs types in autumn samples. These findings highlight the need for improved waste management and sanitation solutions in rural areas to mitigate environmental risks and protect public health. Continued research in these regions is essential to inform effective sanitation strategies. Full article
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23 pages, 4178 KiB  
Article
Taxonomic Biomarkers of Gut Microbiota with Potential Clinical Utility in Mexican Adults with Obesity and Depressive and Anxiety Symptoms
by María Alejandra Samudio-Cruz, Daniel Cerqueda-García, Elizabeth Cabrera-Ruiz, Alexandra Luna-Angulo, Samuel Canizales-Quinteros, Carlos Landa-Solis, Gabriela Angélica Martínez-Nava, Paul Carrillo-Mora, Edgar Rangel-López, Juan Ríos-Martínez, Blanca López-Contreras, Jesús Fernando Valencia-León and Laura Sánchez-Chapul
Microorganisms 2025, 13(8), 1828; https://doi.org/10.3390/microorganisms13081828 - 5 Aug 2025
Abstract
While the gut microbiota of obese children in Mexico has been studied, its relationship with depressive and anxiety symptoms in obese adults remains unexplored. The aim of this study was to describe the gut microbiota profile of Mexican adults with obesity and its [...] Read more.
While the gut microbiota of obese children in Mexico has been studied, its relationship with depressive and anxiety symptoms in obese adults remains unexplored. The aim of this study was to describe the gut microbiota profile of Mexican adults with obesity and its association with depression and anxiety. We sequenced the V3-V4 region of the 16S rRNA gene from stool samples of obese adults categorized into four groups: control (OCG), with depressive symptoms (OD), with anxiety symptoms (OAx), or with both (ODAx). Alpha diversity was assessed using t-tests, beta diversity was assessed with PERMANOVA, and taxonomic differences was assessed with LEfSe. Associations between bacterial genera and clinical variables were analyzed using the Maaslin2 library. Bacteroidota was the most prevalent phylum, and Prevotella was the dominant enterotype across all groups. Although overall diversity did not differ significantly, 30 distinct taxonomic biomarkers were identified among groups as follows: 4 in OCG (Firmicutes), 5 in OD (Firmicutes, Bacteroidota), 13 in OAx (Firmicutes, Bacteroidetes, Fusobacteroidota, Proteobacteria), and 8 in ODAx (Firmicutes). This is the first study to identify distinct gut microbiota profiles in obese Mexican adults with depressive and anxiety symptoms. These findings suggest important microbial biomarkers for improving the diagnosis and treatment of mental health conditions in obesity. Full article
(This article belongs to the Special Issue Gut Microbiota: Influences and Impacts on Human Health)
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22 pages, 982 KiB  
Article
Cross-Cultural Adaptation and Validation of the Spanish HLS-COVID-Q22 Questionnaire for Measuring Health Literacy on COVID-19 in Peru
by Manuel Caipa-Ramos, Katarzyna Werner-Masters, Silvia Quispe-Prieto, Alberto Paucar-Cáceres and Regina Nina-Chipana
Healthcare 2025, 13(15), 1903; https://doi.org/10.3390/healthcare13151903 - 5 Aug 2025
Abstract
Background/Objectives: The social importance of health literacy (HL) is widely understood, and its measurement is the subject of various studies. Due to the recent pandemic, several instruments for measuring HL about COVID-19 have been proposed in different countries, including the HLS-COVID-Q22 questionnaire. The [...] Read more.
Background/Objectives: The social importance of health literacy (HL) is widely understood, and its measurement is the subject of various studies. Due to the recent pandemic, several instruments for measuring HL about COVID-19 have been proposed in different countries, including the HLS-COVID-Q22 questionnaire. The diversity of cultures and languages necessitates the cross-cultural adaptation of this instrument. Thus, the present study translates, adapts, and validates the psychometric properties of the HLS-COVID-Q22 questionnaire to provide its cross-cultural adaptation from English to Spanish (Peru). Methods: As part of ensuring that the final questionnaire accommodates the cultural nuances and idiosyncrasies of the target language, the following activities were carried out: (a) a survey of 40 respondents; and (b) a focus group with 10 participants, followed by expert approval. In addition, the validity and reliability of the health instrument have been ascertained through a further pilot test administered to 490 people in the city of Tacna in southern Peru. Results: The resulting questionnaire helps measure HL in Peru, aiding better-informed decision-making for individual health choices. Conclusions: The presence of such a tool is advantageous in case of similar global health emergencies, when the questionnaire can be made readily available to support a promotion of strategies towards better self-care. Moreover, it encourages other Latin American stakeholders to adjust the instrument to their own cultural, language, and socio-economic contexts, thus invigorating the regional and global expansion of the HL study network. Full article
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