Search Results (68,693)

Transnational youth frequently navigate multiple languages and continually negotiate not only affiliation, but also the legitimacy of the languages they use within changing linguistic hierarchies. However, their educational experiences are often framed through fragmented classroom practices, deficit-based assessments, and nationally bounded curricular frameworks. In this paper, I respond by theorizing Fictional Linguistic Landscapes (FLL) as a transdisciplinary pedagogical approach that utilizes fiction and participatory cultural practices to position language learning as a form of semiotic design, critical inquiry, and identity (re)work. Grounded in linguistic landscape studies, multiliteracies pedagogy, and fan-based meaning-making, FLL positions learners as world-builders and allows them to experiment with visibility, hierarchy, and language(s) in safe fictional environments. This study outlines the four-phase FLL in Second Language Teaching and Learning (L2TL) cycle and provides five pedagogical design spaces to address issues of raciolinguistic valuation, deficit institutional representations, affective harm, peer-level marginalization, and translocal or return migrant identity negotiation. Rather than viewing imagination as an outcome of teaching, FLLinL2TL structures it as a necessary process for learning, linking creative production to explicit linguistic objectives and reflective justification. I conclude by discussing implications for classroom practice, teacher education, and future research on the potential of the FLLinL2TL approach in transnational education research. Full article

Background: Inherited kidney diseases represent a genetically and clinically heterogeneous group of disorders affecting both pediatric and adult populations. Advances in next-generation sequencing (NGS) have improved diagnostic precision; however, genotype–phenotype correlations and diagnostic yield vary substantially across disease entities. Methods:We retrospectively evaluated 165 patients referred for genetic testing due to suspected inherited kidney disease. Patients were classified into three clinical groups: polycystic kidney disease, Alport syndrome, and other syndromic patients with inherited kidney diseases. Genetic analysis was performed using NGS with Human Phenotype Ontology–based gene filtering and included evaluation of both single-nucleotide variants and copy number variations. Results: Overall diagnostic yield differed markedly between groups. A molecular diagnosis was achieved in 71.4% of Alport patients, 41.0% of PKD patients, and 70.2% of patients in the Other syndromic group. In the Alport group, variants were identified exclusively in COL4A3, COL4A4, and COL4A5, with pathogenicity and gene involvement correlating with disease severity and the presence of extrarenal manifestations. The PKD group showed predominant involvement of PKD1, followed by PKHD1 and PKD2, while a substantial proportion of patients remained genetically negative, reflecting technical and biological complexity. The Other group exhibited pronounced genetic heterogeneity, with variants distributed across multiple genes involved in tubular, glomerular, metabolic, and ciliopathy-related pathways. Computational assessments demonstrated that several variants of uncertain significance (VUS) were located in functionally critical domains and were predicted to disrupt protein stability, intermolecular interactions, or conserved structural motifs, thereby supporting the biological plausibility of their potential pathogenic impact. Conclusions: Phenotype-driven NGS enables effective molecular diagnosis across diverse inherited kidney diseases while revealing disease-specific differences in diagnostic yield and genotype–phenotype correlations. Systematic inclusion of variants of uncertain significance and careful integration of genetic and clinical data are essential for accurate interpretation and long-term patient management. Collectively, this study enhances understanding of inherited kidney diseases and underscores the value of integrating comprehensive genomic and computational approaches into routine nephrogenetic practice. Full article

Marine byproducts generated from seafood processing represent valuable reservoirs of structurally and functionally distinct biomolecules, whose composition reflects species, habitat, and processing history. This systematic review identified which marine byproducts have been most extensively studied between 2020 and 2025, with emphasis on their composition, valorisation, and suitability for tracing their geographic origin. Following the PRISMA protocol, 6443 publications were initially retrieved, of which 96 peer-reviewed studies were included for data extraction and analysis. The five most frequently investigated byproducts—skin, bones, scales, shells, and roe—were identified as rich sources of proteins (collagen and gelatin), minerals (hydroxyapatite and calcium carbonate), polysaccharides (chitin), lipids (notably polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)), and vitamin B12. Collagen properties, particularly imino acid content, hydroxylation degree, crosslinking density, and thermal stability, correlate more strongly with environmental temperature than taxonomy, supporting their potential as markers for tracing geographic origin. The mineral fractions, dominated by hydroxyapatite in bones and scales, or calcium carbonate in shells, provided complementary inorganic fingerprints based on calcium-to-phosphorus ratios, carbonate substitution, trace element composition, and thermal analyses. While the lipid profile alone could not completely discriminate fish roe, proteomic techniques, such as MALDI-TOF MS, make it possible to reliably identify species. Collectively, these byproducts offer complementary organic and inorganic markers that support integrated strategies that allow tracing their origin and fostering their sustainable valorisation, overcoming a key technical bottleneck for their use. However, their large-scale conversion into market-ready products remains limited by technical complexity, process variability, and cost-related constraints. Full article

Psoriasis is classically defined as an immune-mediated disease. However, many patients do not achieve durable remission after immune-targeted therapies, suggesting that further pathogenic mechanisms may contribute to the persistence of psoriasis. Here, we propose ferroptosis, an iron-dependent regulated cell death driven by lipid peroxidation and failure of lipid repair, as a potential link between metabolic stress and immune-mediated inflammation in psoriasis. We summarize experimental evidence showing that membrane lipids remodeling, antioxidant suppression, lipid peroxidation, and dysregulated iron handling together define ferroptosis-permissive niches within psoriatic lesions. We also discuss functional studies demonstrating that ferroptosis modulation can reshape psoriasiform inflammation and explore how ferroptotic stress may amplify inflammatory signaling at the immune-epidermal interface, reinforcing IL-17/TNF/IFN-γ pathways. Finally, we discuss ferroptosis-related transcriptomic signatures as a potential approach to stratify psoriasis, capturing metabolic features that are not reflected by cytokine profiling. The translational opportunities and constraints for ferroptosis-targeted interventions are outlined, highlighting epidermal redox homeostasis as a new therapeutic frontier in psoriasis. Full article

Background: There is escalating concern about patient safety among healthcare workers (HCWs) due to the alarming number of deaths and disabilities. Objective: The main aim of this study is to explore the perceptions of patient safety culture (PSC) among HCWs in two major cities in Saudi Arabia, compare the findings with those of international studies, and highlight the major strengths and challenges that affect the incorporation of PSC in these two cities. Methods: A cross-sectional design was used to assess PSC among HCWs in hospitals in Dammam and Jeddah, Saudi Arabia. This study utilised the Saudi Hospital Survey on Patient Safety Culture (HSPSC), which is commonly used by HCWs in the Ministry of Health, and the results were compared with those from the Agency for Healthcare Research and Quality (AHRQ). Data were analysed using the Statistical Package for the Social Sciences (SPSS). Chi-square tests were used to assess the association between patient safety ratings and the reporting of patient safety events. An independent t-test was used to examine differences in mean scores of study variables between the two cities. A p-value of less than 0.05 was considered statistically significant. Results: Out of 737 participants, 357 completed the survey. Physicians were the most common (27%), followed by nurses (11.9%), in Dammam. In Jeddah, nurses were first (20%), followed by transporters and security (12.7 and 11.6%, respectively). Overall, error reporting and supervisor support were areas of strength, while management commitment, teamwork, and incident reporting were identified as areas needing improvement. More than 94% of employees rated patient safety positively. Conclusions: This study highlights the importance of HCWs’ perceptions of PSC in Dammam and Jeddah. Overall, patient safety ratings among participants were highly positive (94%), reflecting favourable views of teamwork, supervisor support, and hospital management’s commitment. Although event reporting and teamwork were recognised as major strengths, it is still necessary to implement regular patient safety training programmes and formal patient safety policies to address existing gaps. Overall, PSC ratings were more positive and significantly higher in Dammam than in Jeddah. These findings may help policymakers and managers enhance patient safety and develop more robust systems to protect both patients and HCWs. Full article

Background: Organisational health literacy (OHL) is increasingly recognised as a system-level strategy to address health literacy-related inequities in healthcare, yet evaluation of practical OHL tools and frameworks remain limited. This study aimed to examine the implementation experiences of the Danish OS! to inform refinements, and to examine the feasibility of the refined version, renamed OHL-Act, in practice. Methods: A two-phase study guided by the RE-AIM framework was conducted. Phase 1 comprised a formative evaluation of OS! based on interviews from previous applications, informing refinement. Phase 2 involved feasibility testing of OHL-Act in a specialised diabetes centre. Results: Across implementing organisations, OS! was experienced as a practical approach supporting reflection and the generation of OHL improvement ideas, while also revealing barriers. These insights informed refinements, including clearer language, more structured facilitation guidance, and explicit prompts addressing health literacy challenges and high-risk situations. Feasibility findings indicated that OHL-Act could be delivered as intended and was perceived as acceptable, relevant, and useful in supporting reflection and the generation of OHL improvement ideas. Conclusions: OHL-Act represents a structured, co-creational approach to support OHL work. Further research is needed to examine how generated improvement ideas translate into sustained action and their potential implications for equity. Full article

Chrysanthemum (Chrysanthemum morifolium) is a globally significant ornamental plant, whose growth, development, and ornamental quality are frequently impaired by salt stress. DOF (DNA-binding with one finger) family transcription factors extensively act as crucial regulators in medicating reactions to environmental pressures on plants. But their specific functions in regulating salt stress tolerance in chrysanthemum still remain largely elusive and require further investigation. Here, we isolated CmDOF2, a DOF family transcription factor from chrysanthemum, whose expression was up-regulated in chrysanthemums under salt stress. Functional analysis demonstrated that CmDOF2 functions as a nuclear-localized transcriptional activator. Comprehensive phenotypic and physiological characterization showed that heterologous expression of CmDOF2 in Arabidopsis thaliana conferred markedly increased salt stress tolerance, as reflected by higher chlorophyll, leaf relative water, and proline content; lower leaf relative electric conductivity and malondialdehyde content; and increased activities of superoxide dismutase, peroxidase, and catalase. Furthermore, qRT-PCR analyses confirmed that stable expression of CmDOF2 in Arabidopsis led to increased transcript levels of key salt-responsive genes, including stress marker genes (AtRD29A, AtRD29B), components of the SOS signaling pathway (AtSOS1, AtSOS2, AtSOS3), genes involved in osmotic adjustment (AtP5CS1, AtP5CS2), and genes encoding antioxidant enzymes (AtSOD1, AtPOD34, AtCAT3). Collectively, our data demonstrate that CmDOF2 serves as a nuclear-localized transcriptional regulator with activation activity and positively regulates salt stress tolerance by mediating the transcript levels of stress-related genes in multiple signaling pathways. Full article

During multi-layer commingled production of coalbed methane (CBM), fluid interference induced by interlayer pressure differences is a major constraint on productivity, representing a dynamic coupling process of reservoir pressure, temperature, and deformation. To elucidate this mechanism, we constructed a four-layer superimposed reservoir physical model using a self-developed large-scale true triaxial multi-field coupling test system, which reflects the geological conditions of the Eastern Yunnan and Western Guizhou region. We precisely regulated interlayer pressure differences and monitoring multi-physical parameters in real time to analyze the dynamic evolution of reservoir temperature, pressure, and deformation fields. The findings reveal that: (1) Increased interlayer pressure difference intensifies fluid interference in low-pressure reservoirs, causing abnormal pressure buildup. For example, when the pressure difference rose from 0.2 MPa to 0.6 MPa, the maximum pressure increase in Reservoir I grew from 1.03 MPa to 1.13 MPa. (2) The high-pressure reservoir (Reservoir IV) remained largely unaffected throughout production, with its temperature decline rate consistently correlated positively with pressure difference, indicating a distinct response behavior. (3) Reservoir deformation correlates positively with initial pressure. When the initial pressure of Reservoir II increased from 1.2 MPa to 1.6 MPa, its volumetric strain rose from 1.81‰ to 2.21‰, attributable to the combined effects of matrix shrinkage, elevated effective stress, and desorption-induced thermal cooling. This study demonstrates how interlayer pressure differences regulate the coupled evolution of reservoir pressure, temperature, and deformation, providing experimental evidence and theoretical support for identifying interference mechanisms and optimizing development strategies in CBM commingled production. Full article

Fatigue damage is a critical factor for the long-term service performance degradation of concrete structures. Nevertheless, the mesoscopic fatigue process is still debatable due to material heterogeneity and the complex internal damage progression. To further investigate the internal damage mechanism of concrete under fatigue loading, this study quantitatively monitors the dynamic internal strain evolution of concrete prismatic specimens during uniaxial compression high-cycle fatigue by designing and embedding aggregate sensors (EAS). The results indicated that EAS may effectively reflect concrete cracking, and the approach can properly capture the internal strain field redistribution features of concrete. Significant internal strain localization was observed during fatigue damage. The turning points in strain evolution, which correlate with the stages of stable propagation and microcrack initiation, were identified. Furthermore, the evolution of internal strain effectively characterized the alteration of stress transfer routes induced by crack propagation. Based on failure modes and mechanical analysis, the synergistic driving mechanism of fatigue damage involving crack growth, interfacial friction and stress field evolution was investigated. The difference in concrete damage under fatigue and monotonic loading due to changing mesoscopic crack propagation was defined, establishing a mechanical foundation for exploring concrete fatigue damage processes. The EAS monitoring method used in this study not only gives a viable approach for the fatigue damage analysis of concrete structures, but it also offers a new viewpoint and data support for comprehending the mesoscopic fatigue mechanism of concrete. Full article

The primary aim of this study is to develop a comprehensive theoretical model for creating digital content that enhances speech formation in toddlers aged 1–3, based on children’s folklore. This model seeks to integrate pedagogical, psychological, and cultural elements to offer a balanced and age-appropriate digital learning experience for young children. The study employed a systematic literature review using Creswell’s seven-step process, which involved identifying relevant research, reviewing and analyzing 22 peer-reviewed studies published between 2019 and 2023, and synthesizing their findings. VOSviewer version 1.6.18, a bibliometric visualization tool, was used to conduct a keyword co-occurrence analysis, identifying key concepts and trends in digital content creation for toddlers. The systematic review adhered to the PRISMA framework to ensure rigor in the selection and analysis of the included studies, which spanned fields such as education, psychology, and pediatric development. The study identified several key dimensions necessary for developing an effective theoretical model of digital content creation for toddlers: The content must be age-appropriate and consider the unique cognitive, linguistic, and developmental needs of toddlers. Children’s folklore plays a crucial role in language development, offering culturally rich and rhythmically engaging material for young learners. The model must address the balance between screen time and real-world interactions, ensuring that digital engagement does not replace essential real-life learning experiences. Ensuring the psychological and physiological safety of digital content is paramount, requiring the exclusion of inappropriate or harmful material and the inclusion of interactive, engaging content that supports speech development. The study concludes that a well-designed model for digital content creation, rooted in children’s folklore, can significantly enhance speech development in toddlers. Such a model must not only support language acquisition but also reflect cultural heritage, promote safe digital environments, and encourage a balance between digital and real-world interactions. By integrating the findings from various disciplines, this theoretical model provides a holistic framework that can guide the development of high-quality digital content aimed at supporting early childhood language development in the digital age. Full article

Pertussis, caused by Bordetella pertussis, remains a public health concern despite long-standing vaccination programs. After a marked decline during the COVID-19 pandemic, a resurgence was observed in Europe and Italy, with a sharp increase in 2024. This study describes pertussis epidemiological trends in the Tuscany Region (Italy) from 2019 to 2024 to identify high-risk groups and inform prevention strategies. A retrospective population-based analysis was conducted using cases reported to the national surveillance system (PREMAL). Incidence rates were calculated using ISTAT population data, and demographic, temporal, and clinical characteristics were analyzed. Overall, 669 cases were reported (mean annual incidence rate: 3.03/100,000 (IC 95% 2.47–3.59; period incidence rate: 18.2/100,000 (IC 95% 16.81–19.56)), with 89% occurring in 2024 (16.34/100,000 (IC 95% 15.03–17.65)). No sex differences were observed, and most cases were reported in Central Tuscany (64%). Children under 15 years accounted for 87% of cases. The highest incidence was observed among 10–14-year-olds, while infants < 1 year, particularly those under 4 months, showed the highest burden in narrower age strata. Hospitalizations occurred in 12.6% of cases, decreasing substantially in 2024. The 2024 resurgence likely reflects waning immunity, disruptions to routine vaccinations during the pandemic, and reduced pathogen circulation in previous years due to containment and isolation measures related to the pandemic. Strengthening surveillance and improving booster and maternal vaccination coverage are essential to protect vulnerable populations. Full article

This study examines prospective teachers’ perceptions of sustainable development (SD) and explores the relationship between the SD scenarios they developed and the actions they performed. Based on 58 scenarios and 128 actions reported by pre-service teachers, the study found that scenarios primarily focused on education, resource conservation, and waste management. While the environmental dimension emphasized protection, economic and social dimensions were largely associated with financial savings and public welfare. In contrast, participants’ actual behaviors were predominantly limited to simple resource conservation, such as turning off lights and saving water. A significant discrepancy emerged: despite the strong emphasis on education-related themes in scenarios, educational actions were notably limited in practice. These results suggest that prospective teachers’ high cognitive awareness of SD is only partially reflected in their practical engagement. The findings highlight a “cognitive–practice gap,” underscoring the need for teacher education programs to prioritize action-oriented competencies. Full article

The prosperity of the Maritime Spice Route in China during the Song–Yuan dynasties (960–1368) propelled Quanzhou into a global hub for maritime trade and cultural integration. A large number of Muslims settled in Quanzhou via maritime routes, living and multiplying over generations—their journey fully documenting the localization trajectory of the immigrant group. To explore the relationship between the evolution of their traditional residence types and immigration localization, this study takes 185 “one bright hall and two dark rooms” traditional residences of the Ding’s Hui ethnic group in Chendai as an example, constructing a “4 × 6” matrix framework via the spatiotemporal biaxial coordinate classification method, with an integrated application of statistics, field surveying and mapping, Space Syntax, and genealogical document analysis. Results reveal that 15 of the 24 theoretical residence types are effectively preserved, forming a “prototype + combined type” evolutionary chain. Residence-type acceptance presents distinct traits, Class A as the foundational form, Class D as the mainstream, and Classes B and C as transitional types, a pattern reflecting the comprehensive influence of construction land conditions, living patterns, and local construction concepts on residence-type selection. Significant disparities in average connectivity between the central courtyard and various core public spaces embody the multi-branch small-family cohabitation mode and verify the localization development trajectory of residential space. The evolution of this residence-type system is confirmed to feature three core characteristics—nonlinearity, integrated and diversified fusion, and spatial constraint—and proposes preservation strategies for double-standard dimensional, multicultural and identifiability qualities, which provide a scientific reference for the protection and renewal of architectural heritage in Hui ethnic communities and similar immigrant settlements on China’s southeast coast. Full article

Understory shrub–grass coverage is a key indicator of forest ecosystem structure and function, and its accurate retrieval via remote sensing is essential for regional ecological assessments. To address the critical limitation in existing multi-angle remote sensing inversion methods: high-resolution images lack angular information while multi-angle datasets suffer from low spatial resolution, making it difficult to achieve large-scale and fine-grained inversion of understory shrub–grass coverage. Here, we developed an inversion method for estimating understory shrub–grass coverage by integrating multi-angle Moderate Resolution Imaging Spectroradiometer data with high-resolution Sentinel-2 imagery to produce 10 m resolution coverage maps; we then used this method to analyze spatiotemporal changes in Changting County from 2016 to 2025. The results demonstrated that the method achieved high accuracy (R² = 0.8418, RMSE = 0.07), meeting the requirements for quantitative understory shrub–grass coverage estimation. Understory shrub–grass coverage exhibited a concentric decreasing pattern from the surrounding mountainous areas toward the central plain, with high-coverage zones concentrated primarily in the west, southwest, and south. Over the 2016–2025 period, understory shrub–grass coverage displayed a fluctuating upward trend: approximately 60% of the study area showed improvement, while 37.73% experienced slight degradation. The change persistence was dominated by positive trends, with an area proportion of 54.14%, which was close to that of the anti-persistent type (44.87%). This study provides technical support for the high-resolution inversion of understory vegetation structure. Full article

Background: Intraoperative high-volume diuresis is a common but under-recognized phenomenon during off-pump coronary artery bypass grafting (OPCABG). Its clinical correlates and implications for perioperative management remain incompletely characterized. Methods: This single-center retrospective cohort study included 1274 adults undergoing elective OPCABG between January and August 2025. High-volume diuresis was defined as urine output ≥ 5 mL·kg⁻¹·h⁻¹. Multivariable logistic regression was used to identify factors independently associated with intraoperative high-volume diuresis. Model discrimination was assessed using the area under the receiver operating characteristic curve (AUC). Results: High-volume diuresis occurred in 39.6% of patients. Older age, hypertension and greater intraoperative fluid infusion were independently associated with high-volume diuresis, whereas preoperative diuretic and greater cumulative exposure to systolic blood pressure < 100 mmHg were inversely associated with diuresis. The multivariable model demonstrated acceptable discrimination (AUC = 0.756). Postoperative outcomes, including acute kidney injury, duration of mechanical ventilation, intensive care unit stay, and hospital length of stay, did not differ between groups. Conclusions: Intraoperative high-volume diuresis during OPCABG reflects complex physiological and hemodynamic responses and can be anticipated based on preoperative and intraoperative factors. These findings support a more individualized interpretation of urine output and perioperative management strategies in OPCABG. Full article