Search Results (76)

Fruit trichomes and pericarp architecture are pivotal for biological defense and postharvest resilience in the genus Actinidia. However, the evolutionary diversity of these structures and the molecular mechanisms governing their development—particularly under the influence of polyploidization—remain poorly understood. We performed a systematic evaluation of 21 Actinidia species and 14 cultivars using scanning electron microscopy (SEM) and histological analysis. To determine the effects of genome doubling, an autotetraploid line was induced from diploid A. chinensis cv. ‘Donghong’, followed by comparative transcriptomic and temporal expression profiling. Morphological characterization identified three distinct evolutionary groups based on fruit surface traits: glabrous, caducous-spotted, and persistent-pubescent. All observed trichomes featured a unique bipartite multicellular architecture. Kiwifruit pericarp thickness (59.8–534.6 μm) was locally reinforced at trichome insertion sites. Among kiwifruit cultivars, polyploidization significantly increased both trichome length and total amount. Transcriptomic analysis revealed 235 differentially expressed genes (DEGs) enriched in hormonal signaling and flavonoid pathways. Two key candidate genes, Achv4p15g023764.t1 and Achv4p01g000003.t1, were identified as candidate genes for stage-specific regulators governing early morphogenesis and late maturation. By characterizing the morphological diversity and genetic underpinnings of Actinidia trichome and epidermal variation, this study establishes a potential scientific framework for the targeted kiwifruit breeding of novel kiwifruit cultivars with optimized fruit surface characteristics. Full article

The codling moth (Cydia pomonella L.) is a major pest of pome fruits worldwide, guided by semiochemicals to locate hosts and oviposition sites. Quince (Cydonia oblonga Mill.), although less studied, is also affected by this pest. This study aimed to identify behaviorally active compounds for codling moth by characterizing the volatilome of quince cultivars. Volatile profiles were analyzed across four phenological stages (flowering, unripe, growth, and ripe fruit) using solid-phase microextraction and GC–MS. The cultivars evaluated were Champion, INTA 37, INTA 117, and INTA 147. Female oviposition behavior and neonate larval host choice were also assessed. Identified volatiles included esters, sesquiterpenes, monoterpenes, alcohols, aldehydes, and norisoprenoids. Among monoterpenes, limonene, consistently detected across all cultivars and stages, emerged as a key kairomone. Volatile composition varied across phenological stages, with the fruit growth stage exhibiting the highest diversity and abundance of compounds previously reported as behaviorally active. This pattern coincided with peak female oviposition and larval host selection. Females oviposited mainly on leaf surface, whereas during ripening, eggs were deposited on fruit lacking pubescence. Overall, INTA 147 was the most preferred cultivar. These findings highlight quince volatiles, particularly Limonene, as potential candidates for the development of semiochemical-based tools to improve codling moth management. Full article

Argyreia elegans Traiperm, Tetsana & Chitchak, sp. nov., from northern Thailand is described and illustrated. The new species resembles A. kerrii Craib but differs in terms of having lanceolate to oblong leaves, up to two inflorescences (each bearing 1–3 flowers emerging from an axillary bud), and outer sepals that are densely velvety-pubescent on the abaxial side. A detailed description, an updated key to the species for the Flora of Thailand, as well as anatomical, micromorphological, and taxonomic notes are provided. Full article

Boesenbergia burmanica Boonma, P.Saensouk & Saensouk, a new species from Naypyidaw Union Territory, Myanmar, is described based on morphological examination of specimens collected during field surveys in 2024–2025. The genus Boesenbergia is taxonomically complex, and ongoing botanical exploration in Southeast Asia continues to reveal undescribed taxa. Comparative analyses of living plants, herbarium materials, and published descriptions were conducted to determine diagnostic features. Boesenbergia burmanica is closely related to B. meghalayensis Aishwarya & M.Sabu but differs in having ovoid, branched rhizomes, a pubescent lamina on both surfaces, and an oblanceolate labellum with bright red markings. It grows in shady, sandy-loam habitats at 140–145 m elevation and flowers from August to September, producing inflorescences with nine to ten flowers. Species of Boesenbergia are traditionally used in Myanmar and neighboring regions as medicinal and ornamental plants, and notes on local ethnobotanical knowledge are provided. The new species exhibits high ornamental potential due to its compact clumping habit and attractive floral coloration. Cultivation guidelines are provided to support ex situ conservation and horticultural development for sustainable use. This discovery increases the number of Boesenbergia species recorded in Myanmar to 14 and highlights the importance of continued field-based taxonomic and conservation research on regional Zingiberaceae diversity. Full article

A new species of Bauhinia from the Brazilian Cerrado is described. Based on floral morphology, it can be assigned to Bauhinia sect. Pauletia ser. Cansenia, but its vegetative characters distinguish it from all other known members of the group. To elucidate its taxonomic position, we conducted detailed morphological comparisons, and molecular phylogenetic analyses using the nuclear ribosomal ITS region and two plastid markers (matK and trnL–F). Bayesian Inference (BI) and Maximum Likelihood (ML) analyses were performed. Our results support (both PP from BI and BP from ML) the placement of this new species within Bauhinia ser. Cansenia. The new species named B. latistipulata sp. nov. is easily distinguished from other species of Bauhinia ser. Cansenia by its reniform stipules and cymbiform bracts. The species shows morphological similarities to B. ungulata, sharing elliptic to ovate leaf blades with obtuse apices, tomentose ovaries, and clavate stigmas. However, it differs by its hirsute abaxial leaf surface (vs. tomentose), tubular and ribbed flower buds (vs. clavate or subclavate and smooth), tomentose staminal tube (vs. pubescent), and distinct color of the ovary indumentum (white vs. ferruginous). Full article

During hybrid soybean seed production, the parents’ phenotypic consistency is assessed by breeders to ensure the purity of soybean seeds. Detection traits encompass the hypocotyl, leaf, pubescence, and flower. To achieve the detection of hybrid soybean parents’ phenotypic consistency in the field, a self-propelled image acquisition platform was used to obtain soybean plant image datasets. In this study, the Large Selective Kernel Network (LSKNet) attention mechanism module, the detection layer Small Network (SNet), dedicated to detecting small objects, and the Wise Intersection over Union v3 (WIoU v3) loss function were added into the YOLOv5s network to establish the hybrid soybean parent phenotypic consistency detection model SLW-YOLO. The SLW-YOLO achieved the following: F1 score: 92.3%; mAP: 94.8%; detection speed: 88.3 FPS; and model size: 45.1 MB. Compared to the YOLOv5s model, the SLW-YOLO model exhibited an improvement in F1 score by 6.1% and in mAP by 5.4%. There was a decrease in detection speed by 42.1 FPS, and an increase in model size by 31.4 MB. The parent phenotypic consistency detected by the SLW-YOLO model was 98.9%, consistent with manual evaluation. Therefore, this study demonstrates the potential of using deep learning technology to identify phenotypic consistency in the seed production of large-scale hybrid soybean varieties. Full article

Understanding the genetic architecture of complex traits is crucial for crop improvement and molecular breeding. We developed a mutagenized soybean population using carbon ion beam irradiation and conducted genome-wide association studies (GWAS) to identify variants controlling key agronomic traits. Whole-genome resequencing of 199 M4 lines revealed 1.48 million SNPs, predominantly C→T transitions, with population structure analysis identifying three distinct genetic groups. GWAS across five traits revealed striking differences in genetic architecture: the podding habit showed extreme polygenic control with 87,029 significant associations of small effect, while pubescence color exhibited oligogenic inheritance with only 122 variants. Hundred-seed weight displayed moderate complexity (4637 associations) with the largest effect sizes (−3.74 to 5.03) and major QTLs on chromosomes 4, 7, and 15–20. Growth habit involved 12,136 SNPs, including a strong chromosome 3 association (−log₁₀(p-value) > 50). Flower color showed 2662 associations clustered on chromosome 15. Functional analysis of 18,542 candidate genes revealed trait-specific pathway enrichments: flavonoid biosynthesis for flower color, phloem transport for seed weight, auxin signaling for growth habit, and amino acid transport for podding habit. This study demonstrates how mutagenesis-induced variation, combined with association mapping, reveals evolutionary constraints that shape genetic architectures, providing insights for genetics-assisted breeding strategies. Full article

Background/Objectives: Leaf sheath hairiness (LSH) is an adaptive trait in wheat that improves tolerance to biotic and abiotic stresses. Although trichome development has been extensively studied in model plants, the genetic basis of LSH in Triticeae crops remains poorly defined. Methods: In this study, the inheritance and genetic architecture of LSH were investigated. Two F₂ populations were used, derived from crosses between the glabrous lines ‘Shumai 830’ and ‘Shumai 2262’ and the hairy line ‘Zhongkelanmai 1’. BSA-seq was combined with KASP marker genotyping to map and refine the trait locus. Candidate genes were evaluated through comparative genomics; sequence variation; and subcellular localization prediction. Results: Phenotypic evaluation revealed that LSH is a dominant trait, segregating at a 3:1 ratio in F₂ populations. BSA-seq identified a major locus, QLsh.cwnu-4D, on chromosome 4DL. Fine mapping with KASP markers refined this region to a 1.67 Mb interval overlapping a 530 kb trichome-associated linkage disequilibrium block in Aegilops tauschii. Within this interval, TaSAIN1-4D, a salt-inducible protein unique to Triticeae, was identified as the strongest candidate gene. Extensive sequence variation among alleles (TaSAIN1-4Da; TaSAIN1-4Db; TaSAIN1-4Dc), including large insertions and multiple SNPs, indicated potential functional diversification. Predicted nuclear localization of TaSAIN1-4D supports a role in trichome regulation and stress adaptation. The co-dominant KASP marker K-cwnu-4D-502238348 was tightly linked to LSH and cosegregated perfectly, making it a reliable tool for marker-assisted selection. Conclusions: This study clarifies the genetic architecture of leaf sheath hairiness in wheat and identifies TaSAIN1-4D as a likely regulator. These findings provide a practical marker-assisted selection tool that can accelerate the development of improved wheat varieties with desirable leaf surface traits. Full article

This paper provides a brief review of the genus Trichorondonia Breuning, 1965. A new species, Trichorondonia wenkaii sp. nov. (文凯毛郎氏天牛), is described and illustrated. Trichorondonia kabateki Viktora, 2024 is newly recorded in Hubei province, with the first description of the male. Additionally, photographs of the holotypes of three previously described species are presented. A key to the four species is given. The new species differs from T. pilosipes and T. hybolasioides in having elytra with rounded lateral apical angles and a vertex with blackish-brown pubescence medially behind the eyes. The new species can also be easily distinguished from T. kabateki by the antennae being ventrally fringed with sparse hairs only on segments 1–8, the greyish-yellow pubescence on the pronotum being unevenly distributed and particularly sparse in the posterior half, the elytra having rather thin greyish-yellow pubescence and hardly visible greyish-white pubescence, elongated blackish-brown spots on the elytral longitudinal carinae, and a small tuft of black setae at the centre of the elytral base where there is no obvious tubercle. The type specimen of the new species was collected in Dianping village, Xinhua town, Leye county, Guangxi Zhuang Autonomous Region of China, and deposited at Insect Collection, College of Agriculture, Yangtze University, Jingzhou, Hubei, China (ICYZU). Full article

In the context of global warming, which is mainly due to the increasing atmospheric concentration of carbon dioxide, the prediction of climate change requires a good assessment of the involvement of vegetation in the global carbon cycle. In particular, determining when vegetative activity ceases in deciduous forests remains a great challenge. Remote sensing of solar-induced fluorescence (SIF) has been considered as a potential proxy for ecosystem photosynthesis and, therefore, a relevant indicator of the end of the vegetation period as compared to other vegetation indices, such as EVI (Enhanced Vegetation Index) and NDVI (Normalized Difference Vegetation Index). However, many challenges remain to be addressed due to the lack of knowledge of the response of SIF at different time scales, different species and different environmental conditions. The aim of this study was to explore the diurnal and seasonal variations in the SIFA and SIFB signals in a pubescent oak forest undergoing senescence. We show that apparent SIFA yield may be considered an earlier indicator of the end of the vegetation period compared to NDVI, which primarily reflects the ratio of SIFB/SIFA. The apparent SIFA yield signal was positively and highly correlated with PRI (Photochemical Reflectance Index), EVI and NDVI. Air contents in CO₂ and O₃ were similarly significantly correlated to SIFs emission but only during the growth phase of the phenology of Q. pubescens. At the seasonal scale, the results show that SIF variations were mainly driven by variations in PAR, air VPD and temperature. A higher dependence of the SIF signal on these last three variables was observed at the diurnal scale through Pearson correlation coefficients, which were greater than seasonal ones. Full article

Within the Rubiaceae family, the genus Randia comprises approximately 90 species, with significant morphological diversity in growth forms, leaf shapes, and floral sexuality. In particular, Randia aculeata is a taxonomically challenging species distributed from the southern United States to South America and the Antilles that exhibits high morphological variability and ecological adaptability. One of its synonyms is Randia tomatillo, originally described from coastal dunes in Veracruz, Mexico, and it has been inconsistently described in the taxonomic literature either as a distinct species or as a synonym for R. aculeata. This study aimed to resolve the taxonomic identity of R. tomatillo through morphological observations from herbarium specimens, and transverse sections from mature leaves were observed using SEM microscopy to explore crystal types. Morphological traits, such as lifeform, twig pubescence, stipule persistence, fruit size, and pericarp texture, are critical in distinguishing R. tomatillo from morphologically related species, such as R. aculeata and Randia thurberi. Anatomical features, particularly the presence of prisms in the leaf mesophyll and epidermis, are unique to R. tomatillo, suggesting their diagnostic value. Ecological adaptations, such as scandent growth and spongy pericarps for hydrochorous fruit dispersal, further support its distinction as a species adapted to coastal environments. These findings highlight the need for a comprehensive taxonomic reassessment of Randia, integrating morphological, anatomical, and ecological evidence. Full article

The Sapotaceae family comprises 65–70 genera and over 1250 species, holding significant ecological and economic value. Although previous studies have made some progress in the phylogenetic relationships and classification of Sapotaceae, many issues remain unresolved and require further in-depth research. In this study, we sequenced and assembled the complete chloroplast genomes of 21 plants from 11 genera of Sapotaceae, conducted a comparative genomic analysis, and performed a phylogenetic analysis by incorporating 16 previously published chloroplast genomes of Sapotaceae. The results showed that the chloroplast genome sizes in 21 plants of Sapotaceae range between 157,920 bp and 160,130 bp, exhibiting the typical quadripartite structure. Each genome contains 84–85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes, while the ndhF gene is absent in Pouteria campechiana and Pouteria sapota. The relative synonymous codon usage (RSCU) analysis showed that isoleucine (Ile) is the most commonly used, while the codon for methionine (Met) is the least utilized. Additionally, five highly variable regions (petA-psbJ, psbI-trnS-GGA, rpl2_2-psbA, rps19-rpl2_2, and ycf4-cemA) and two coding sequences, ycf1 and matK, were identified as candidate molecular markers for species differentiation and a phylogenetic analysis within the Sapotaceae family. Phylogenetic trees were reconstructed using complete chloroplast genome sequences and analyzed using ML and BI methods, which revealed that the Sapotaceae family is divided into three distinct clades, each receiving strong statistical support (BS = 100, PP = 1). The intergeneric analysis revealed that Madhuca and Palaquium are sister groups (BS = 91, PP = 1), as are Gambeya and Chrysophyllum (BS = 91, PP = 1). Pouteria and Chrysophyllum are among the larger groups in the Sapotaceae family but the traditional classification boundaries of these genera are unstable and unfeasible, as the current genus boundaries fail to support their natural evolutionary relationships. In the phylogenetic tree, Eberhardtia aurata is placed on a separate branch. The morphological classification system shows that E. aurata has rust-colored pubescence on its branches, abaxial leaf surfaces, petioles, and other areas, which clearly distinguishes it from other genera. This study provides valuable insights into advancing phylogenetic research, population genetics, molecular breeding, and conservation strategies by comparing chloroplast genome structures and characteristics and constructing phylogenetic trees. Full article

A new species of Drymaria from the Kohima District (Nagaland of North-East India) is described and illustrated based on both molecular data (the ITS region of nuclear DNA) and morphometric analyses (clustering, PCA, DA, and MANOVA). The new species resembles D. cordata but differs with respect to the shape of the sepal (oblong, incurved at the margin vs. lanceolate, not incurved at the margin), petals (oblong or linear vs. ovate–lanceolate), and bract (foliaceous, glabrous, non-prominent vs. non-foliaceous, pubescent, and prominent). For nomenclatural purposes, the typification of Drymaria villosa by Duke (in 1961) was corrected according to Art. 9.10 of the Shenzhen Code. Full article

Introduction. Melatonin and serotonin can influence certain aging processes in the ovaries. The main melatonin receptors are represented by types MT1 and MT2. The goal of investigation. Here, we evaluated the expression of genes and synthesis of MT1 and MT2 receptors, as well as serotonin synthesis in the ovaries during ontogenesis. Methods. We analyzed histological material obtained from the ovaries of infants, women of younger and older reproductive age, premenopausal, menopausal, and postmenopausal women. For the analysis of MT1 and MT2 receptors and serotonin expression and synthesis, RT-PCR and immunohistochemistry were used. Results. We found that the synthesis of serotonin, as well as MT1 and MT2 receptors in the ovaries significantly decrease in ontogenesis. The sharpest drop in these molecules was observed in samples obtained from one-year-old infants, as well as from pubescent girls and menopausal women. A statistically significant 2.3–7.6-fold decrease in the expression of MTNR1A and MTNR1B genes in the ovaries was also observed in one-year-old infants, in adolescents, and in middle-aged women. Conclusions. These data are crucial to understanding the fundamental mechanisms of aging of the female reproductive system and the search for molecules predicting its aging. Full article

To reduce negative effects of floral visitation by ants, which do not serve as reliable cross-pollinators, some plants have developed a non-floral, stem-based defense mechanism called greasy pole syndrome. In the present study, we examined the effects of two surface features (trichomes and three-dimensional epicuticular wax coverage) on stems of Alliaria petiolata plants on visiting frequencies, travelled distances, and running velocities of Lasius niger ants. The experiments were performed with stem samples prepared from different (apical and basal) stem portions showing different surface morphologies (smooth control, covered by wax and trichomes + wax, respectively). The control, mechanically wiped stem samples lacking any surface features were significantly more often visited by ants, where they travelled significantly longer distances and moved with significantly higher velocities, compared to the intact stems. The apical and basal stem portions showed no significant differences in the measured parameters. Based on data obtained, we conclude about the main contribution of the wax to the greasy pole function of the A. petiolata stem via reduction of ant adhesion to the wax-bearing stem surface, whereas trichomes presumably serve as the first barrier for ants approaching usually from the ground level and protect the fragile wax coverage from an excessive deterioration. Full article