Melatonin Receptors and Serotonin: Age-Related Changes in the Ovaries
Abstract
:1. Introduction
2. Materials and Methods
2.1. Characteristics of Experimental Groups
- Group 1: infants under 12 months (antenatal) (n = 10);
- Group 2: 2–4-year-old infants (n = 5),
- Group 3: 5–11-year-old girls (n = 5),
- Group 4: 12–15-year-old girls (n = 11),
- Group 5: women of the younger reproductive ages of 16–18 (n = 20),
- Group 6: women of the reproductive ages of 19–29 (n = 23),
- Group 7: women of the older reproductive ages of 30–44 (n = 24),
- Group 8: premenopausal and menopausal women of ages 45–59 (n = 21),
- Group 9: menopausal women of ages 60–74 (n = 19),
- Group 9: postmenopausal women of ages 60–74 (n = 10),
- Group 10: postmenopausal women of ages 75–89 (n = 10),
- Group 11: postmenopausal women of ages 90–92 (n = 6).
2.2. Immunofluorescence Staining
2.3. Morphometrical Analysis
2.4. Statistical Processing
2.5. PCR
3. Results
3.1. MT1, MT2, and Serotonin Expression in Human Ovarian Tissue from Different Age Groups
3.1.1. Results of the Immunofluorescence Analysis of Ovarian Tissue
3.1.2. Results of MTNR1A and MTNR1B PCR Analysis of Ovarian Tissue
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Sequence |
---|---|
MTNR1A | Forward 5′-TGGTTTTGTTGGGTGAAAAG-3′ |
Reverse 5′-CTACCCTTACCCTACATAATCCCTATAC-3′ | |
MTNR1B | Forward 5′-AGTTGGGTAGGGAAGAGA-3′ |
Reverse 5′-AACCCCATACCAACACCCAACAT-3′ |
Age, y.o. | Me (Q1; Q3) | ||
---|---|---|---|
MT1 | MT2 | Serotonin | |
Under 12 months | 44.59 (38.89; 46.61) | 43.66 (43.05; 44.39) | 17.36 (16.13; 18.27) |
1 | 30.01 (29.26; 31.41) | 26.72 (26.26; 27.46) | 30.03 (27.91;30.41) |
2–4 | 19.50 (18.00; 20.16) | 15.71 (15.06; 16.11) | 15.58 (14.90; 16.71) |
5–11 | 14.86 (14.28; 15.38) | 10.47 (9.40; 10.91) | 14.31 (13.40;14.89) |
12–15 | 10.71 (10.25; 11.39) | 6.12 (5.99; 6.92) | 17.28 (16.88; 17.79) |
16–18 | 7.51 (6.98; 7.94) | 3.72 (3.38; 4.29) | 18.14 (17.67; 18.40) |
19–29 | 3.36 (2.42; 4;56) | 2.41 (2.21; 2.50) | 11.75 (11.01;12.83) |
30–44 | 4.36 (3.62; 5.84) | 2.25 (1.97; 2.56) | 6.35 (5.77; 6.55) |
45–59 | 3.32 (2.92; 4.25) | 1.44 (1.34; 1.54) | 3.07 (2.41; 3.34) |
60–74 | 2.56 (2.41; 3.21) | 1.16 (1.03; 1.19) | 1.57 (1.34; 2.14) |
75–89 | 1.69 (1.45; 1.78) | 0.54 (0.49; 0.65) | 0.69 (0.62; 0.87) |
>90 | 0.76 (0.36; 1.05) | 0.25 (0.11; 0.46) | 0.26 (0.17; 0.29) |
Age, y.o. | Me (Q1; Q3) | ||
---|---|---|---|
MT1 | MT2 | Serotonin | |
under 12 months | 29.77 (29.04; 31.00) | 47.57 (44.68; 48.60) | 27.07 (24.05; 29.87) |
1 | 15.60 (14.90; 16.94) | 35.97 (34.24; 29.31) | 25.45 (23.16; 26.89) |
2–4 | 10.30 (9.11; 10.94) | 23.02 (20.09; 24.63) | 42.24 (38.94; 45.21) |
5–11 | 7.90 (7.11; 8.06) | 14.40 (13.59; 14.86) | 21.35 (19.77; 24.80) |
12–15 | 5.82 (5.73; 6.33) | 12.07 (11.63; 13.93) | 28.77 (24.18; 31.03) |
16–18 | 5.06 (4.86; 5.40) | 10.54 (9.76; 11.76) | 41.08 (39.61; 42.89) |
19–29 | 3.84 (3.37; 4.14) | 5.9 (5.52; 6.83) | 21.04 (16.79; 23.66) |
30–44 | 2.82 (2.41; 3.36) | 3.89 (3.30; 4.30) | 12.47 (11.19; 13.44) |
45–59 | 1.84 (1.59; 2.18) | 2.67 (2.51; 2.83) | 7.21 (6.64; 8.03) |
60–74 | 0.98 (0.92; 1.05) | 2.00 (1.78; 2.78) | 4.88 (4.42; 5.42) |
75–89 | 0.61 (0.52; 0.68) | 1.80 (1.39; 2.30) | 3.39 (2.35; 3.60) |
>90 | 0.34 (0.30; 0.37) | 0.43 (0.13; 0.63) | 0.83 (0.37; 1.57) |
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Polyakova, V.; Medvedev, D.; Linkova, N.; Mushkin, M.; Muraviev, A.; Krasichkov, A.; Dyatlova, A.; Ivanova, Y.; Gullo, G.; Gorelova, A.A. Melatonin Receptors and Serotonin: Age-Related Changes in the Ovaries. J. Pers. Med. 2024, 14, 1009. https://doi.org/10.3390/jpm14091009
Polyakova V, Medvedev D, Linkova N, Mushkin M, Muraviev A, Krasichkov A, Dyatlova A, Ivanova Y, Gullo G, Gorelova AA. Melatonin Receptors and Serotonin: Age-Related Changes in the Ovaries. Journal of Personalized Medicine. 2024; 14(9):1009. https://doi.org/10.3390/jpm14091009
Chicago/Turabian StylePolyakova, Victoria, Dmitrii Medvedev, Natalia Linkova, Mikhail Mushkin, Alexander Muraviev, Alexander Krasichkov, Anastasiia Dyatlova, Yanina Ivanova, Giuseppe Gullo, and Anna Andreevna Gorelova. 2024. "Melatonin Receptors and Serotonin: Age-Related Changes in the Ovaries" Journal of Personalized Medicine 14, no. 9: 1009. https://doi.org/10.3390/jpm14091009