Search Results (29,155)

Atherosclerosis (AS) is a leading cause of death and disability in type 2 diabetes mellitus (T2DM). However, the shared molecular mechanisms linking T2DM and atherosclerosis have not been fully elucidated. We analyzed AS- and T2DM-related gene expression profiles from the Gene Expression Omnibus (GEO) database to identify overlapping differentially expressed genes and co-expression signatures. Functional enrichment (Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG)) and protein–protein interaction (PPI) network analyses were then used to describe the pathways and interaction modules associated with these shared signatures, We next applied the cytoHubba algorithm together with several machine learning methods to prioritize hub genes and evaluate their diagnostic potential and combined CIBERSORT-based immune cell infiltration analysis with single-cell RNA sequencing data to examine cell types and the expression patterns of the shared genes in specific cell populations. We identified 72 shared feature genes. Functional enrichment analysis of these genes revealed significant enrichment of inflammatory- and metabolism-related pathways. Three genes—IL1B, MMP9, and P2RY13—emerged as shared hub genes and yielded robust ANN-based predictive performance across datasets. Immune deconvolution and single-cell analyses consistently indicated inflammatory amplification and an imbalance of macrophage polarization in both conditions. Biology mapped to the hubs suggests IL1B drives inflammatory signaling, MMP9 reflects extracellular-matrix remodeling, and P2RY13 implicates cholesterol transport. Collectively, these findings indicate that T2DM and AS converge on immune and inflammatory processes with macrophage dysregulation as a central axis; IL1B, MMP9, and P2RY13 represent potential biomarkers and therapeutic targets and may influence disease progression by regulating macrophage states, supporting translational application to diagnosis and treatment of T2DM-related atherosclerosis. These findings are preliminary. Further experimental and clinical studies are needed to confirm their validity, given the limitations of the present study. Full article

Enzyme engineering drives innovation in biotechnology, medicine, and industry, yet conventional approaches remain limited by labour-intensive workflows, high costs, and narrow sequence diversity. Artificial intelligence (AI) is revolutionising this field by enabling rapid, precise, and data-driven enzyme design. Machine learning and deep learning models such as AlphaFold2, RoseTTAFold, ProGen, and ESM-2 accurately predict enzyme structure, stability, and catalytic function, facilitating rational mutagenesis and optimisation. Generative models, including ProteinGAN and variational autoencoders, enable de novo sequence creation with customised activity, while reinforcement learning enhances mutation selection and functional prediction. Hybrid AI–experimental workflows combine predictive modelling with high-throughput screening, accelerating discovery and reducing experimental demand. These strategies have led to the development of synthetic “synzymes” capable of catalysing non-natural reactions, broadening applications in pharmaceuticals, biofuels, and environmental remediation. The integration of AI-based retrosynthesis and pathway modelling further advances metabolic and process optimisation. Together, these innovations signify a shift from empirical, trial-and-error methods to predictive, computationally guided design. The novelty of this work lies in presenting a unified synthesis of emerging AI methodologies that collectively define the next generation of enzyme engineering, enabling the creation of sustainable, efficient, and functionally versatile biocatalysts. Full article

This study proposes a novel two-dimensional Empirical Mode Decomposition (2D EMD)-based deep learning framework to enhance model performance in multi-class image classification tasks and potential early detection of diseases in healthcare using medical imaging. To validate this approach, we apply it to gastrointestinal (GI) endoscopic image classification using the publicly available Kvasir dataset, which contains eight GI image classes with 1000 images each. The proposed 2D EMD-based design procedure decomposes images into a full set of intrinsic mode functions (IMFs) to enhance image features beneficial for AI model development. Integrating 2D EMD into a deep learning pipeline, we evaluate its impact on four popular models (ResNet152, VGG19bn, MobileNetV3L, and SwinTransformerV2S). The results demonstrate that subtracting IMFs from the original image consistently improves accuracy, F1-score, and AUC for all models. The study reveals a notable enhancement in model performance, with an approximately 9% increase in accuracy compared to counterparts without EMD integration for ResNet152. Similarly, there is an increase of around 18% for VGG19L, 3% for MobileNetV3L, and 8% for SwinTransformerV2. Additionally, explainable AI (XAI) techniques, such as Grad-CAM, illustrate that the model focuses on GI regions for predictions. This study highlights the efficacy of 2D EMD in enhancing deep learning model performance for GI image classification, with potential applications in other domains. Full article

Wire Arc Additive Manufacturing (WAAM), also known as Wire Arc Directed Energy Deposition, is used for fabricating large metallic components with high deposition rates. However, the process often leads to residual stress, distortion, defects, undesirable microstructure, and inconsistent bead geometry. These challenges necessitate reliable in-situ monitoring for process understanding, quality assurance, and control. While several reviews exist on in-situ monitoring in other additive manufacturing processes, systematic coverage of sensing methods specifically tailored for WAAM remains limited. This review fills that gap by providing a comprehensive analysis of existing in-situ monitoring approaches in WAAM, including thermal, optical, acoustic, electrical, force, and geometric sensing. It compares the relative maturity and applicability of each technique, highlights the challenges posed by arc light, spatter, and large melt pool dynamics, and discusses recent advances in real-time defect detection and control, process monitoring, microstructure and property prediction, and minimization of residual stress and distortion. Apart from providing a synthesis of the existing literature, the review also provides research needs, including the standardization of monitoring methodologies, the development of scalable sensing systems, integration of advanced AI-driven data analytics, coupling of real-time monitoring with multi-physics modeling, exploration of quantum sensing, and the transition of current research from laboratory demonstrations to industrial-scale WAAM implementation. Full article

This paper discusses the evolution of convolutional (CNN) and recurrent (RNN) artificial neural networks in applications for Building Information Modeling (BIM). The paper outlines the milestones reached in the last two decades. The article organizes the current state of knowledge and technology in terms of three aspects: (1) computer visualization coupled with BIM models (detection, segmentation, and quality verification in images, videos, and point clouds), (2) sequence and time series modeling (prediction of costs, energy, work progress, risk), and (3) integration of deep learning results with the semantics and topology of Industry Foundation Class (IFC) models. The paper identifies the most used architectures, typical data pipelines (synthetic data from BIM models, transfer learning, mapping results to IFC elements) and practical limitations: lack of standardized benchmarks, high annotation costs, a domain gap between synthetic and real data, and discontinuous interoperability. We indicate directions for development: combining CNN/RNN with graph models and transformers for wider use of synthetic data and semi-/supervised learning, as well as explainability methods that increase trust in AECOO (Architecture, Engineering, Construction, Owners & Operators) processes. A practical case study presents a new application, Bimetria, which uses a hybrid CNN/OCR (Optical Character Recognition) solution to generate 3D models with estimates based on two-dimensional drawings. A deep review shows that although the importance of attention-based and graph-based architectures is growing, CNNs and RNNs remain an important part of the BIM process, especially in engineering tasks, where, in our experience and in the Bimetria case study, mature convolutional architectures offer a good balance between accuracy, stability and low latency. The paper also raises some fundamental questions to which we are still seeking answers. Thus, the article not only presents the innovative new Bimetria tool but also aims to stimulate discussion about the dynamic development of AI (Artificial Intelligence) in BIM. Full article

Latent tuberculosis infection (LTBI) represents a biologically active yet clinically asymptomatic stage of Mycobacterium tuberculosis (Mtb) persistence. This condition is characterized by subtle immunometabolic alterations reflecting the host–pathogen equilibrium. Understanding the mechanisms and biomarkers associated with the preclinical phase of LTBI is crucial for preventing progression to active tuberculosis (ATB). Recent advances have identified multiple immunological, transcriptomic, metabolic, and imaging-based approaches that enable stratification of individuals at increased risk of LTBI reactivation. Quantitative assays such as IGRA, multiplex and T-cell activation marker (TAM) tests, as well as interferon-related transcriptional signatures, demonstrate predictive potential when combined with functional assays (MGIA) and metabolic imaging (PET/CT). Experimental primate models faithfully reproduce the spectrum from latency to reactivation, allowing for validation of biomarkers and vaccine or immunomodulatory strategies. The review also highlights the particular challenges of multidrug-resistant LTBI (MDR-LTBI), where standard chemoprophylaxis is less effective and immune control plays a decisive role. The preclinical phase of LTBI constitutes a key point in the TB control cascade. Integrating immunological, transcriptomic, and radiological data into risk-based screening algorithms could substantially improve early detection and targeted prevention. Translating research-derived signatures into clinically applicable, standardized, and cost-effective diagnostic tools requires coordinated international efforts, technological transfer, and policy-level support to reduce TB reactivation and transmission, including MDR-TB. Full article

Tight reservoirs are highly heterogeneous, with complex pore-throat structures and varying fluid occurrences. The Archie equation shows a nonlinear relationship, making traditional logging interpretation methods unreliable for accurately predicting water saturation. This paper employs particle swarm optimization (PSO), using Pearson correlation coefficient-based feature selection, to compare the accuracy of three machine learning algorithms: XGBoost, LightGBM, and MERF in predicting water saturation in tight reservoirs. It also applies the SHAP value algorithm to provide a visual and interpretive analysis of the PSO LightGBM model. The research results indicate that the root mean square error (RMSE), coefficient of determination (R2), and accuracy of water saturation (Swa) of the PSO-LightGBM model on the training and test sets are 0.955, 3.087, 91.8%, and 0.89, 5.132, 85.2%, respectively. Interpretability analysis using SHAP values reveals that the five normalized logging parameters—SP, M2R3, DEN, DT, and CN—are the most influential features in the water saturation prediction model. In application examples involving water saturation prediction across eight sections of tight reservoirs in the study area, the PSO–LightGBM, PSO–XGBoost, and PSO–MERF models achieved Swa of 88.9%, 80.3%, and 87.8%, respectively. The results demonstrate that the PSO–LightGBM model is a reliable and efficient method for predicting water saturation, with significant practical potential. Full article

This review examines how the integration of circular bioeconomy principles with digital technologies can drive climate change mitigation, improve resource efficiency, and facilitate sustainable biorefinery development. This highlights the urgent need to transition away from fossil fuels and introduces the bio-circular economy as a regenerative model focused on biomass valorization, reuse, recycling, and biodegradability. This study compares linear, circular, and bio-circular approaches and analyzes key policy frameworks in Europe, Latin America, and Asia linked to several UN Sustainable Development Goals. A central focus is the role of digitalization, particularly artificial intelligence (AI), the Internet of Things (IoT), and blockchain. Examples include AI-based biomass yield prediction and biorefinery optimization, IoT-enabled real-time monitoring of material and energy flows, and blockchain technology for supply chain traceability and transparency. Applications in agricultural waste valorization, bioplastics, bioenergy, and nutraceutical extraction are also discussed in this review. Sustainability tools, such as automated life-cycle assessment (LCA) and Industry 4.0 integration, are outlined. Finally, future perspectives emphasize autonomous smart biorefineries, biotechnology–nanotechnology convergence, and international collaboration supported by open data platforms. Full article

The development of KRAS G12D inhibitors represents an effective therapeutic strategy for treating oncological pathologies. Existing quantitative structure-activity relationship (QSAR) models for KRAS G12D inhibitors have several limitations, primarily the lack of applicability domain determination and virtual screening implementation. In this study, we propose a set of regression QSAR models for KRAS G12D inhibitors by employing various molecular descriptors and machine learning methods. Our consensus model achieved a Q² test value of 0.70 on an external test set, covering 78% of the data within the applicability domain. We integrated this consensus model into our Python-based framework KRASAVA. The platform predicts inhibitory activity while considering the applicability domain, assesses compounds for compliance with Muegge’s bioavailability rules, and identifies PAINS, toxicophores, and Brenk filters. Furthermore, we structurally interpreted the QSAR models to propose several promising inhibitors and performed molecular docking on these candidates using GNINA. For the reference inhibitor MRTX1133, we reproduced the crystal structure pose with an RMSD of 0.76 Å (PDB ID: 7T47). The key interactions with amino acid residues Asp12, Asp69, His95, Arg68, and Gly60, identified for both MRTX1133 and our proposed compounds, demonstrate a strong consistency between the molecular docking and QSAR results. Full article

Real-world decision-making often involves uncertainty, incomplete data, and the need to evaluate alternatives based on both quantitative and qualitative criteria. To address these challenges, this study presents FAS-XAI, a unified methodological framework that integrates fuzzy clustering and explainable artificial intelligence (XAI). FAS-XAI supports interpretable, data-driven decision-making by combining three key components: fuzzy clustering to uncover latent behavioral profiles under ambiguity, supervised prediction models to estimate decision outcomes, and expert-guided interpretation to contextualize results and enhance transparency. The framework ensures both global and local interpretability through SHAP, LIME, and ELI5, placing human reasoning and transparency at the center of intelligent decision systems. To demonstrate its applicability, FAS-XAI is applied to a real-world B2B customer service dataset from a global ERP software distributor. Customer engagement is modeled using the RFID approach (Recency, Frequency, Importance, Duration), with Fuzzy C-Means employed to identify overlapping customer profiles and XGBoost models predicting attrition risk with explainable outputs. This case study illustrates the coherence, interpretability, and operational value of the FAS-XAI methodology in managing customer relationships and supporting strategic decision-making. Finally, the study reflects additional applications across education, physics, and industry, positioning FAS-XAI as a general-purpose, human-centered framework for transparent, explainable, and adaptive decision-making across domains. Full article

This study aims to analyze text data obtained from Turkish phone calls to manage credit risk in the banking sector and predict whether customers will fulfill their payment promises. Data cleaning was identified as a critical step to improve the quality of the texts, and various natural language processing (NLP) techniques were used. The model was built using a two-layer LSTM architecture, starting with a Self-Embedding layer, and achieved approximately 80% accuracy on the test data. The findings indicate that customers who break their payment promises often cite personal life issues such as health problems, family issues, financial difficulties, and religious beliefs to ensure reliability. These results demonstrate the importance of text data in the banking sector, the applicability of different embedding methods to Turkish datasets, and their advantages and disadvantages. Furthermore, the model built using data obtained from customer conversations can help predict credit risk more accurately and contribute to improving call center processes. Automating data cleaning processes and developing speech-to-text translation tools are recommended for future studies. Full article

Accurate, real-time monitoring of meat freshness is essential for reducing food waste and safeguarding consumer health, yet conventional methods rely on costly, laboratory-grade spectroscopy or destructive analyses. This work presents a low-cost electronic-nose platform that integrates a compact array of metal-oxide gas sensors (Figaro TGS2602, TGS2603, and Sensirion SGP30) with a Gaussian Process Regression (GPR) model to estimate a continuous freshness index under refrigerated storage. The pipeline includes headspace sensing, baseline normalization and smoothing, history-window feature construction, and probabilistic prediction with uncertainty. Using factorial analysis and response-surface optimization, we identify history length and sampling interval as key design variables; longer temporal windows and faster sampling consistently improve accuracy and stability. The optimized configuration (≈143-min history, ≈3-min sampling) reduces mean absolute error from ~0.51 to ~0.05 on the normalized freshness scale and shifts the error distribution within specification limits, with marked gains in process capability and yield. Although it does not match the analytical precision or long-term robustness of spectrometric approaches, the proposed system offers an interpretable and energy-efficient option for short-term, laboratory-scale monitoring under controlled refrigeration conditions. By enabling probabilistic freshness estimation from low-cost sensors, this GPR-driven e-nose demonstrates a proof-of-concept pathway that could, after further validation under realistic cyclic loads and operational disturbances, support more sustainable meat management in future smart refrigeration and cold-chain applications. This study should be regarded as a methodological, laboratory-scale proof-of-concept that does not demonstrate real-world performance or operational deployment. The technical implications described herein are hypothetical and require extensive validation under realistic refrigeration conditions. Full article

This article provides a narrative review of the technical, computational and clinical aspects of transcranial brain stimulation (TBS), with an emphasis on transcranial magnetic stimulation (TMS) and transcranial direct current stimulation (tDCS). The review addresses three central questions: which physical, engineering, and biological principles determine the generation, propagation, and focality of electromagnetic fields in the human head. The second question asks how modeling approaches, stimulation parameters, and hardware design influence the accuracy, safety, and individual variability of brain stimulation. And, finally, how these technical factors translate into current clinical applications, therapeutic efficacy, and practical limitations. The key take-home messages are as follows: for engineers, realistic anatomical head models, precise coil/electrode placement, and reliable numerical solvers remain essential for predicting field distribution and optimizing stimulation protocols; for clinicians, stimulation outcomes are strongly dependent on anatomy-specific field patterns, safety constraints, and device-related parameters that require careful adjustment; and for both groups, despite significant technological progress, effective and reproducible stimulation still demands systematic protocol refinement and individualized planning. Overall, this review integrates contemporary technical knowledge with clinical perspectives to support evidence-based use and future development of TMS and tDCS. Full article

Kiwifruit (Actinidia spp.) is valued for its sensory quality and nutritional richness but faces postharvest challenges such as rapid softening, chilling injury, and physiological disorders. Conventional management strategies help maintain quality yet insufficient to capture the complexity of ripening, stress physiology, and cultivar-specific variation. Recent research emphasizes the continuum from preharvest to postharvest, where orchard practices, harvest maturity, and handling conditions influence quality and storage potential. Omics-driven studies, particularly transcriptomics and metabolomics, have revealed molecular networks regulating softening, sugar–acid balance, pigmentation, antioxidant properties, and chilling tolerance. Integrated multi-omics approaches identify key biomarkers and gene–metabolite relationships linked to ripening and stress responses. Complementing omics, nondestructive estimation technologies, including hyperspectral imaging, near-infrared spectroscopy, acoustic profiling, and chemometric models are emerging as practical tools for real-time classification of maturity, quality, and storability. When calibrated with omics-derived biomarkers, these technologies provide predictive, non-invasive assessments that can be deployed across the supply chain. Together, the convergence of postharvest physiology, omics, and nondestructive sensing offers a pathway toward precision quality management and sustainable kiwifruit production. This review synthesizes recent advances across these domains, highlighting mechanistic insights, practical applications, and future directions for integrating omics-informed strategies with commercial postharvest technologies. Full article

Sustainable renovation is a critical aspect for designing energy-efficient buildings with reasonable cost and high indoor living standards. The objective of this paper is to investigate various renovation scenarios for an old, uninsulated building with a floor area of 100 m² located in Athens, aiming to determine the global optimal solution through a multi-criteria analysis. The multi-criteria analysis considers energy, economic, and thermal comfort criteria to perform a multi-lateral approach. Specifically, the criteria are: (i) maximization of the energy savings, (ii) minimization of the life cycle cost (LCC), and (iii) minimization of the mean annual predicted percentage of dissatisfied (PPD). These criteria are combined within a multi-criteria evaluation procedure that employs a global objective function for determining a global optimum solution. The examined retrofitting actions are the addition of external insulation, the replacement of the existing windows with triple-glazed windows, the addition of shading in the openings in the summer, the application of cool roof dyes, the use of a mechanical ventilation system with a heat recovery unit, and the installation of a highly efficient heat pump system. The interventions were examined separately, and the combined renovation scenarios were studied by including them in the external insulation because of their high importance. The present study encompassed the investigation of a baseline scenario and 26 different renovation scenarios, conducted through dynamic simulation on an annual basis. The results of the present analysis indicated that the global optimal renovation scenario, including the addition of external insulation, the installation of highly efficient heat pumps, and the use of shading in the openings in the summer, saved energy by 74% compared to the baseline scenario. The LCC was approximately EUR 33,000, the simple payback period of the renovation process was around 6 years, the annual CO₂ emissions avoidance reached 4.6 tn_CO2, and the PPD was at 9.7%. An additional sensitivity analysis for determining the optimal choice under varying weights assigned to the criteria revealed that this renovation design is the most favorable option in most cases. These results prove that the suggested renovation scenario is a feasible and viable solution that leads to a sustainable design from multiple perspectives. Full article