An extension of neo-Darwinism, termed preassembly, states that genetic material required for many complex traits, such as echolocation, was present long before emergence of the traits. Assembly of genes and gene segments had occurred over protracted...
The outer and inner dynein arms (ODAs and IDAs) are composed of multiple subunits including dynein heavy chains possessing a motor domain. These complex structures are preassembled in the cytoplasm before being transported to the cilia. The molecular...
A study of cryogenic drilling in sandwich composites was carried out. The materials used were carbon-fiber-reinforced polymer sandwich sheets with an inner foamed polyvinyl chloride core, composites with applications including protection structures o...
Four natural phenomena are cited for their defiance of conventional neo-Darwinian analysis: human intelligence; cat domesticity; the Cambrian explosion; and convergent evolution. 1. Humans are now far more intelligent than needed in their hunting&nda...
Shortly after entering the cells, cytomegaloviruses (CMVs) initiate massive reorganization of cellular endocytic and secretory pathways, which results in the forming of the cytoplasmic virion assembly compartment (AC). We have previously shown that t...
Established hairpin stators for electric traction motors are made up of a large number of so-called hairpins. To produce these stators, the individual hairpins must first be pre-assembled into an auxiliary device in order to achieve the desired windi...
Background/Objectives: Transgene applications, ranging from gene therapy to the development of stable cell lines and organisms, rely on maintaining the expression of transgenes. To date, the use of plasmid-based transgenes has been limited by the los...
The mounting of pre-assembled cables with electrical connectors is mainly carried out manually in industry today. An exemplary application is the interconnection of battery modules. Automation of such assembly tasks offers the potential for increasin...
This article proposes a framework for the development of process digital twins (DTs) in the shipbuilding sector, based on the ISO 23247 standard and structured around the achievement of three levels of digital maturity. The framework is demonstrated...
In this study, 220 AA7075-T6 B-pillars were fabricated using a thoroughly instrumented hot-stamping press under varied conditions. Feature engineering work identified nineteen attributes of the hot-stamping process as impacting four characteristics o...
This paper presents a novel approach to developing fully automated intelligent control systems for use within production-based organizations, with a specific focus on advancing research into intelligent production systems. This analysis underscores a...
Titanium aluminide (TiAl) turbine blades produced by isothermal forging have recently been implemented in the low-pressure part of commercial aircraft jet engines. However, the slow speed of isothermal forging, costly molybdenum-based dies and the re...
Murine cytomegalovirus (MCMV) initiates the stepwise establishment of the pre-assembly compartment (pre-AC) in the early phase of infection by the expansion of the early endosome (EE)/endosomal recycling compartment (ERC) interface and relocation of...
Promyelocytic leukemia (PML) nuclear bodies (PML-NBs) are core–shell-type membrane-less organelles typically found in the nucleus of mammalian somatic cells but are absent in mouse oocytes. Here, we deliberately induced the assembly of PML-NBs...
The digitalization of shipbuilding processes has become an important trend in modern naval construction, enabling more efficient design, assembly, and maintenance operations. A key aspect of this digital transformation is traceability, which ensures...
Guanine crystals with unique optical properties in organisms have been extensively studied and the biomineralization principles of guanine are being established. This review summarizes the fundamental physicochemical properties (solubility, tautomers...
The physiochemical properties of nanomaterials have a close relationship with their status in solution. As a result of its better simplicity than that of pre-assembled aggregates, the in situ assembly of nanomaterials has been integrated into the des...
While alkaloids often exert unique pharmacological effects on animal cells, exposure of sea urchin eggs to nicotine causes polyspermy at fertilization in a dose-dependent manner. Here, we studied molecular mechanisms underlying the phenomenon. Althou...
Robotics is increasingly entering the field of education. The tools, methods, and approaches of robotics contribute to the development of all areas of STEAM education, both individually and interdisciplinary. The present work aims to highlight the ro...
The inactivation of photosensitizers before they reach the targeted tissues can be an important factor, which limits the efficacy of photodynamic therapy (PDT). Here, we developed co-assembled nanohybrids of graphene oxide (GO) and albumin/photosensi...
Many strategies have been adopted to prepare silica materials with highly controlled structures, typically using sol–gel chemistry. Frequently, the alkoxysilanes used in sol–gel chemistry are based on monoalcohols, e.g., Si(OEt)4. The str...
Emerging infectious diseases represent an increasing threat to human and animal health. Therefore, safe and effective vaccines that could be available within a short time frame after an outbreak are required for adequate prevention and control. Here,...
This research study attempts to prove the concept of the applicability of hyperthermia to treating the lysozyme amyloid fibrils (LAFs)’s self-assembled fibrillary aggregates by a feedback-modulated temperature controller ranging from 26 °C...
Some straw buildings, which combine eco-sustainability with versatility, low cost, and fast construction times, have recently been built in Northern Italy. In this work, the technologies used to build straw houses are presented, and the characteristi...
Offshore wind energy has achieved significant reductions in its levelized cost of energy (LCoE) in the past decade, but still needs efficiency improvements. Approximately 18% of the LCoE is related to logistical costs, underscoring the need for optim...
Space robotic systems tend to be more flexible and equipped with some appendages, such as solar panels and communication antennas. The inevitable vibration in the flexible appendage will occur during space missions, and it in turn affects the attitud...
A Matrigel-based tube formation assay is a simple and widely accepted 2D angiogenesis model in vitro. Extracellular matrix (EM) proteins and growth factors (GFs) from MatrigelTM exclusively trigger endothelial cell (EC) tubular network (ETN) formatio...
The global push for sustainable energy has heightened the demand for green hydrogen, which is crucial for decarbonizing heavy industry. However, current electrolysis plant capacities are insufficient. This research addresses the challenge through opt...
(1) Background: Demographic changes over the past decade have had a significant impact on pelvic ring fractures. They have increased dramatically in the orthogeriatric population. Surgeons are faced with implant fixation issues in the treatment of th...
The preparation of an electroactive matrix for the immobilization of the bioprobe shows great promise to construct the label-free biosensors. Herein, the electroactive metal-organic coordination polymer has been in-situ prepared by pre-assembly of a...
The antiapoptotic protein Bcl-xL is a major regulator of cell death and survival, but many aspects of its functions remain elusive. It is mostly localized in the mitochondrial outer membrane (MOM) owing to its C-terminal hydrophobic α-helix. In...
When isolated from mammalian cell nuclei, all nuclear pre-mRNAs are packaged in multi-subunit large ribonucleoprotein complexes—supraspliceosomes—composed of four native spliceosomes interconnected by the pre-mRNA. Supraspliceosomes contain all five...
Signal transducer and activator of transcription (STAT) plays an essential role in the inflammatory reaction and immune response of numerous renal diseases. STATs can transmit the signals of cytokines, chemokines, and growth factors from the cell mem...
BACH1 (BTB And CNC Homology 1) and NRF2 (Nuclear Factor Erythroid 2-related Factor 2) are transcription factors that regulate antioxidant and iron metabolism genes by competing for binding to cis-regulatory Maf-binding elements (CsMBEs) as heterodime...
This paper presents the performance of a new, floating, mono-hull wind turbine installation vessel (Nordic Wind) in the installation process. The vessel can transport pre-assembled wind turbines from the marshalling port to the offshore installation...
Double-stranded DNA viruses package their genomes into pre-assembled protein procapsids. This process is driven by macromolecular motors that transiently assemble at a unique vertex of the procapsid and utilize homomeric ring ATPases to couple genome...
The self-assembly of tau into paired helical filaments (PHFs) in neurofibrillary tangles (NFTs) is a significant event in Alzheimer’s disease (AD) pathogenesis. Numerous post-translational modifications enhance or inhibit tau assembly into NFTs. Oxid...
The assembly quality of an aero-engine directly determines its stability in high-speed operation. The coaxiality and unbalance out of tolerance caused by improper assembly may give rise to complicated vibration faults. To meet the requirements of the...
The frozen elephant trunk technique (FET) requires the use of a pre-assembled hybrid prosthesis consisting of a standard Dacron vascular portion to replace the aortic arch and a stent graft component, which is placed into the proximal descending thor...
Background: Primary ciliary dyskinesia (PCD) is a rare genetic disorder characterized by recurrent sinopulmonary infections due to motile cilia defects. The disease is genetically heterogeneous, with abnormalities in structural ciliary proteins. Zinc...
Infection with cytomegalovirus (CMV) is highly prevalent in the general population and largely controlled by CD8pos T cells. Intriguingly, anti-CMV T cells accumulate over time to extraordinarily high numbers, are frequently present as tumor-resident...
We present a simulation study of supramolecular aggregates formed by three-arm azobenzene (Azo) stars with a benzene-1,3,5-tricarboxamide (BTA) core in water. Previous experimental works by other research groups demonstrate that such Azo stars assemb...
This paper presents an integrated planner based on rapidly exploring random tree (RRT) for an assembly task with possible re-grasping. Given multiple grasp poses for the part to assemble, the planner chooses candidate grasp poses considering the envi...
Adenosine triphosphate (ATP) cleavage powers packaging of a double-stranded DNA (dsDNA) molecule in a pre-assembled capsid of phages that include T3. Several observations constitute a challenge to the conventional view that the shell of the capsid is...
Photonic packaging, which includes high-precision assembly of photonic sub-systems, is currently a bottleneck in the development of commercially-available integrated photonic products. In the pursuit of a fully-automated, high-precision, and cost-eff...
In circular production lines, issues such as positional errors, manufacturing errors at different square groove workstations, and the accumulation of errors during continuous assembly reduce the assembly success rate and limit efficiency. To address...
Photoacoustic imaging (PAI) integrates the high-contrast merits of optical imaging with the high-spatial-resolution advantages of acoustic imaging, enabling the acquisition of three-dimensional images with deep tissue penetration (up to several centi...
CRISPR-Cas9 technology has opened new perspectives in genome editing of clonally, asexually propagated and polyploid plants by enabling multiple allelic gene edits. Traditional Agrobacterium- and particle bombardment-mediated transformations, which r...
DNA origami nanostructures are widely employed in various areas of fundamental and applied research. Due to the tremendous success of the DNA origami technique in the academic field, considerable efforts currently aim at the translation of this techn...
Capsid virus-like particles (cVLPs) are used as molecular scaffolds to increase the immunogenicity of displayed antigens. Modular platforms have been developed whereby antigens are attached to the surface of pre-assembled cVLPs. However, it remains u...
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