Search Results (3,013)

Super-resolution microscopy (SRM) has revolutionized our understanding of subcellular structures, including cell organelles and viruses. For human immunodeficiency virus (HIV), SRM has significantly advanced knowledge of viral structural biology and assembly dynamics. This review analyzes how SRM techniques (particularly PALM, STORM, STED, and SIM) have been applied over the past decade to study HIV structural components and assembly. By categorizing and comparing studies based on SRM methods, HIV components, and labeling strategies, we assess the strengths and limitations of each approach. Our analysis shows that PALM is most commonly used for live-cell imaging of HIV Gag, while STED is primarily used to study the viral envelope (Env). STORM and SIM have been applied to visualize various components, including Env, capsid, and matrix. Antibody labeling is prevalent in PALM and STORM studies, targeting Env and capsid, whereas fluorescent protein labeling is mainly associated with PALM and focused on Gag. A recent emphasis on Gag and Env points to deeper investigation into HIV assembly and viral membrane dynamics. Insights from SRM studies of HIV not only enhance virological understanding but also inform future research in therapeutic strategies and delivery systems, including extracellular vesicles. Full article

This study investigates the improvement effect and micro-mechanism of guar gum and palm fibers, two eco-friendly materials, on expansive soil. The study uses disintegration tests, unconfined compressive strength tests, triaxial compression tests, and SEM analysis to evaluate the enhancement of mechanical properties. The results show that the guar gum–palm fiber composite significantly improves the compressive and shear strength of expansive soil. The optimal ratio is 2% guar gum, 0.4% palm fiber, and 6 mm palm fiber length. Increasing fiber length initially boosts and then reduces unconfined compressive strength. Guar gum increases unconfined compressive strength by 187.18%, further improved by 20.9% with palm fibers. When fiber length is fixed, increasing palm fiber content increases and then stabilizes peak stress and shear strength (cohesion and internal friction angle), improving by 27.30%, 52.1%, and 12.4%, respectively, compared to soil improved with only guar gum. Micro-analysis reveals that guar gum enhances bonding between soil particles via a gel matrix, improving water stability and mechanical properties, while palm fibers reinforce the soil and inhibit crack propagation. The synergistic effect significantly enhances composite-improved soil performance, offering economic and environmental benefits, and provides insights for expansive soil engineering management. Full article

Introduction: Myotonia is a rare neuromuscular condition characterized by impaired muscle relaxation. In this study, we provide normative values for clinical tests related to myotonia in the Hungarian population and compare them to patients with myotonic dystrophy type 1 (DM1). Methods: Relaxation tests (10 eye openings, tongue extension, and palm openings), handgrip strength, and the nine-hole peg test were conducted on 139 healthy individuals and 31 patients with DM1. Results: We observed non-significant declines in handgrip strength and relaxation tests with age (p < 0.05). Significant differences were found between controls (n:139) and patients with DM1 (n = 31) in all tests (p < 0.05). Sex differences were noted in the healthy population: men (n:68/139) had stronger handgrip (mean of men 42.45 ± 1.15 vs. women 24.3 ± 0.9) and slower relaxation tests (mean of eye openings in men 3.6 ± 0.2 vs. in women 4.2 ± 0.2, tongue extensions in men 3.7 ± 0.2 vs. in women 4.2 ± 0.2, palm openings in men 4 ± 0.2 vs. in women 4.9 ± 0.2 However, these differences were not present among patients with DM1. Discussion: Normal values for relaxation tests across different age groups were established. These results might be useful for further clinical investigations. Our study supports the usage of averages of healthy population instead of age groups of relaxation tests and their clinical relevance in the evaluation of patients with myotonia. Full article

The study aimed to identify potential sources of novel probiotic yeasts exhibiting health-promoting properties. A combination of metabarcoding analysis and cultural methods was employed to investigate and isolate yeasts from various sources, including rice wine, palm wine, fermented shrimp paste at different stages of natural fermentation, and lychee peels. The two analytical methods revealed distinct yeast profiles, and each source exhibited a unique composition of yeast species. Through metabarcoding and cultural methods, it was demonstrated that lychee peels harbored a greater diversity of genera compared to other sources. The evaluation of the probiotic properties of yeasts revealed that lychee peel yielded the highest proportion of isolates with potential probiotic activity (53.33%), followed by palm wine (25%), fermented shrimp paste (10%), and rice wine (9.09%). Moreover, yeast isolates with health-promoting properties as evaluated in this study, including Starmerella meliponinorum L12 and Pichia terricola L9 from lychee peels, demonstrated notable antioxidant activity and cholesterol-reducing properties, respectively. These findings represent the first report providing initial insights into the influence of yeast sources and serve as a guideline for the targeted selection of yeasts with specific probiotic and health-promoting attributes. Full article

In this study, we introduce the application of deep learning (DL) models, specifically convolutional neural networks (CNNs), for detecting the health status of date palm leaves using images captured by an unmanned aerial vehicle (UAV). The images are modeled using the Newton–Euler method to ensure stability and accurate image acquisition. These deep learning models are implemented by a voting-based classification (VBC) system that combines multiple CNN architectures, including MobileNet, a handcrafted CNN, VGG16, and VGG19, to enhance classification accuracy and robustness. The classifiers independently generate predictions, and a voting mechanism determines the final classification. This hybridization of image-based visual servoing (IBVS) and classifiers makes immediate adaptations to changing conditions, providing straightforward and smooth flying as well as vision classification. The dataset used in this study was collected using a dual-camera UAV, which captures high-resolution images to detect pests in date palm leaves. After applying the proposed classification strategy, the implemented voting method achieved an impressive accuracy of 99.16% on the test set for detecting health conditions in date palm leaves, surpassing individual classifiers. The obtained results are discussed and compared to show the effectiveness of this classification technique. Full article

Studying the nutritional ecology of Neoseiulus cucumeris (Oudemans) at different temperatures is a fundamental tool for improving mass production for use in biological control of pest mites. The current research studied the impact of both food types and temperatures on the life history and demographic parameters of the predator mite N. cucumeris. Mite cultures in the laboratory were developed using Tetranychus urticae Koch, and N. cucumeris was collected from field plants. The developmental stages of N. cucumeris fed on date palm pollen and the immature stages of T. urticae were investigated in a laboratory setting at different temperatures. Our research revealed that N. cucumeris readily accepted both food types at all the tested temperatures. The developmental stages and adult longevity of N. cucumeris, both female and male, decreased dramatically when the temperature increased from 18 °C to 34 °C. The net reproductive rate (R₀) reached its greatest values of 22.52 and 9.72 offspring/individual at 26 °C, and the intrinsic rate of increase (r_m) reached its maximum values of 0.17 and 0.13 day⁻¹ at 34 °C and minimum of 0.12 and 0.10 day⁻¹ at 18 °C, when fed on date palm pollen and immature stages of T. urticae, respectively. Conversely, the average generation time (T) showed a notable reduction from 22.48 to 16.48 and 20.88 to 16.76 days, accompanied by an upsurge in temperature from 18 °C to 34 °C, when fed on date palm pollen and immature stages of T. urticae, respectively. The finite rate of growth (λ) exhibited distinct variations, reaching its highest value at 34 °C, 26 °C, and 18 °C when fed on date palm pollen and immature stages of T. urticae, respectively. From these results, we can conclude that N. cucumeris was successfully fed date palm pollen as an alternate source of nourishment. In addition, the immature stages of T. urticae are suitable as food sources for N. cucumeris because they shorten the mean generation time. Therefore, the success of mass-rearing the predator mite N. cucumeris on a different, less expensive diet, such as date palm pollen, and determining the most suitable temperature for it has increased its spread as a biocontrol agent. Full article

The escalating demand for sustainable and eco-friendly production processes has necessitated the exploration of renewable resources for the synthesis of valuable chemicals. This study investigated the fermentative synthesis of gluconic acid (GA) and xylonic acid (XA) from hydrolyzed palm fronds by using Gluconobacter oxydans. The key variables examined included agitation speed, inoculum ratio, and composition of fermentation media. In a synthetic medium, maximum GA concentration reached 52.82 ± 12.88 g/L at 65 h using 150 rpm agitation and 15% (v/v) inoculation, while maximum XA concentration achieved 2.31 ± 1.43 g/L at 96 h using 220 rpm agitation and 9% (v/v) inoculation. In the hydrolysate medium, the maximum GA concentration was 3.24 ± 0.66 g/L at fermentation onset using 220 rpm agitation and 15% (v/v) inoculation, while the maximum XA concentration reached 0.62 ± 0.04 g/L at 24 h using 190 rpm agitation and 5% (v/v) inoculation. These findings demonstrate the feasibility of utilizing palm fronds as a renewable feedstock for the sustainable synthesis of high-value biochemicals, promoting waste valorization, and contributing to the advancement of a circular bioeconomy. Full article

Background: Scrub typhus (ST) is caused by Orientia tsutsugamushi (OT) infection, which is transmitted to humans through the bites of infected chiggers. The clinical presentations range from mild to life-threatening multi-organ dysfunction. This report describes a cluster of ST cases involving five oil palm estate workers in Pekan district, Pahang, Malaysia. Methods: The clinical history, laboratory, and entomological investigation were conducted on the patients, including the index case and four suspected cases in the cluster. Polymerase chain reaction (PCR) tests for OT and genotyping were performed on the patients’ blood and urine samples. Serological testing by indirect immunoperoxidase (IIP) test against Rickettsial diseases was also conducted. Principal Findings: Patients presented with fever, myalgia, headache, rash, cough, and eschar. The index case developed severe ST complicated by acute kidney injury (AKI) and respiratory distress, requiring intubation and ventilation at the intensive care unit of a tertiary hospital. ST was confirmed through PCR analysis of a urine sample, showcasing a novel diagnostic approach. The other four cases were confirmed by a four-fold rise in immunoglobulin G (IgG) antibody titers. Conclusions: oil palm estate workers are at high risk for chigger exposure in Malaysia. Awareness among clinicians and the public of ST is crucial for effective prevention, accurate diagnosis, and optimal management. Full article

Sustainable Development Reserves are organized units in the Amazon that are essential for the proper use and sustainable management of the region’s natural resources and for the livelihoods and economy of the local communities. This study aims to provide a climatic characterization of the Madeira River Sustainable Development Reserve (MSDR), offering scientific support to efforts to assess the feasibility of implementing adaptation measures to increase the resilience of isolated Amazon communities in the face of extreme climate events. Significant statistical analyses based on time series of observational and reanalysis climate data were employed to obtain a detailed diagnosis of local climate variability. The results show that monthly mean two-meter temperatures vary from 26.5 °C in February, the coolest month, to 28 °C in August, the warmest month. Monthly precipitation averages approximately 250 mm during the rainy season, from December until May. July and August are the driest months, August and September are the warmest months, and September and October are the months with the lowest river level. Cold spells were identified in July, and warm spells were identified between July and September, making this period critical for public health. Heavy precipitation events detected by the R80, Rx1day, and Rx5days indices show an increasing trend in frequency and intensity in recent years. The analyses indicated that the MSDR has no potential for wind-energy generation; however, photovoltaic energy production is viable throughout the year. Regarding the two major commercial crops and their resilience to thermal stress, the region presents suitable conditions for açaí palm cultivation, but Brazil nut production may be adversely affected by extreme drought and heat events. The results of this study may support research on adaptation strategies that includethe preservation of local traditions and natural resources to ensure sustainable development. Full article

Background: Huriez syndrome is a rare hereditary skin disorder marked by early-onset sclerodactyly, hyperkeratosis of the palms and soles, and nail dysplasia. A key concern is the early and aggressive development of cutaneous squamous cell carcinoma (SCC), typically affecting the dorsal aspects of the hands. Methods: This narrative review summarizes clinical features, genetic aspects, and oncologic implications of Huriez syndrome. A systematic search was conducted in PubMed and Scopus, including English-language articles published up to May 2025. Relevant case reports and small case series were analyzed. Results: Seven patients (58.3%) underwent multiple surgeries due to recurrent or bilateral disease. Six patients (50%) required amputations, including finger, hand, and arm amputations, with no foot amputations reported. Reconstruction after oncological resection was performed in six patients (50%) using skin grafts (3), pedicled flaps (2), or free flaps (1). Amputation was mainly for advanced disease, with radial forearm flaps used for reconstruction. All flaps remained disease-free. Five cases (41.6%) had a history of local recurrence. Conclusions: The early diagnosis of Huriez syndrome is crucial to enable the surveillance and timely treatment of SCC. A multidisciplinary team including dermatologists, oncologists, plastic surgeons, and geneticists is recommended. Further research is needed to clarify genetic mechanisms and develop early detection strategies to improve outcomes. Full article

This research analyzes the influence of hydroxy on pure diesel and blends of palm oil and coffee husk biodiesel with percentages of 15% and 20%. The experimental tests were carried out in a stationary diesel engine, where the torque and speed varied from 3–7 Nm and 3000–3600 rpm. Hydroxy was used as a secondary fuel with a volumetric flow injection of 4 and 8 lpm. The injection of hydroxy can reduce the BSFC and increase the BTE of the engine when running on pure diesel and biodiesel blends. The results show a maximum decrease of 11.66%, 11.28%, and 10.94% in BSFC when hydroxy is injected into D100, D85P10C5, and D80P10C10 fuels. In the case of BTE, maximum increases of 13.37%, 12.84%, and 12.34% were obtained for the above fuels. The fuels D100 + 8 lpm, D85P10C5 + 8 lpm, and D80P10C10 + 8 lpm achieved maximum energy efficiencies of 28.16%, 27.58%, and 27.32%, respectively. In the case of exergy efficiency, maximum values of 26.39%, 25.83%, and 25.58% were obtained. The environmental and social costs of CO, CO₂, and HC emissions are significantly reduced with the addition of hydroxy in pure diesel and biodiesel blends from palm oil and coffee husk. The injection of a volumetric flow rate of 8 l/min results in reductions of 11.66%, 10.61%, and 10.94% in operational cost when the engine is fueled with D100, D85P10C5, and D80P10C10, respectively, complying with standards essential for safe engine operation. In general, the research conducted indicates that hydroxy injection is a viable alternative for reducing fuel consumption and improving engine efficiency when using biodiesel blends made from palm oil and coffee husk. Full article

The palm-vein recognition system has garnered attention as a biometric technology due to its resilience to external environmental factors, protection of personal privacy, and low risk of external exposure. However, with recent advancements in deep learning-based generative models for image synthesis, the quality and sophistication of fake images have improved, leading to an increased security threat from counterfeit images. In particular, palm-vein images acquired through near-infrared illumination exhibit low resolution and blurred characteristics, making it even more challenging to detect fake images. Furthermore, spoof detection specifically targeting palm-vein images has not been studied in detail. To address these challenges, this study proposes the Fourier-gated feature-fusion network (FGFNet) as a novel spoof detector for palm-vein recognition systems. The proposed network integrates masked fast Fourier transform, a map-based gated feature fusion block, and a fast Fourier convolution (FFC) attention block with global contrastive loss to effectively detect distortion patterns caused by generative models. These components enable the efficient extraction of critical information required to determine the authenticity of palm-vein images. In addition, fractal dimension estimation (FDE) was employed for two purposes in this study. In the spoof attack procedure, FDE was used to evaluate how closely the generated fake images approximate the structural complexity of real palm-vein images, confirming that the generative model produced highly realistic spoof samples. In the spoof detection procedure, the FDE results further demonstrated that the proposed FGFNet effectively distinguishes between real and fake images, validating its capability to capture subtle structural differences induced by generative manipulation. To evaluate the spoof detection performance of FGFNet, experiments were conducted using real palm-vein images from two publicly available palm-vein datasets—VERA Spoofing PalmVein (VERA dataset) and PLUSVein-contactless (PLUS dataset)—as well as fake palm-vein images generated based on these datasets using a cycle-consistent generative adversarial network. The results showed that, based on the average classification error rate, FGFNet achieved 0.3% and 0.3% on the VERA and PLUS datasets, respectively, demonstrating superior performance compared to existing state-of-the-art spoof detection methods. Full article

This study evaluated the influence of different male date palm cultivars, distinguished by their chloroplast haplotypes, on pollen quality, pollination efficiency, metaxenia effects, and gene expression during fruit development. Chloroplast DNA analysis of 37 male trees revealed multiple haplotypes, from which cultivars B25, P8, C22, and B46 were selected for further investigation. Pollen viability varied significantly among cultivars, with P8 and B25 exhibiting the highest germination rates and pollen tube elongation, while C22 showed the lowest. These differences correlated with pollination success: P8 and B25 achieved fertilization rates near 99%, whereas C22 remained below 43%. Pollination outcomes also varied in fruit traits. Despite its low pollen performance, C22 induced the production of larger fruits at the Bleh (Kimri) stage, potentially due to compensatory physiological mechanisms. Phytochemical profiling revealed significant cultivar effects: fruits from B25-pollinated trees had with lower moisture and polyphenol content but the higher sugar levels and soluble solids, suggesting accelerated maturation. Ripening patterns confirmed this finding, with B25 promoting the earliest ripening and B46 causing the most delayed. Gene expression analysis supported these phenotypic differences. Fruits pollinated by P8, B25, and B46 exhibited elevated levels of cell-division-related transcripts, particularly the PdCD_1 gene (PDK_XM_008786146.4, a gene encoding a cell division control protein), which was most abundant in P8. In contrast, fruits from C22-pollinated trees had the lowest expression of growth-related genes, suggesting a shift toward cell expansion rather than division. Overall, the results show the critical role of male genotype in influencing fertilization outcomes and fruit development, offering valuable insights for targeted breeding strategies at enhancing date palm productivity and fruit quality. Full article

Palm oil is a highly versatile natural resource that has gathered significant attention due to its bioactive properties, particularly its antimicrobial and antioxidant effects. Rich in tocotrienols, tocopherols, and carotenoids, palm oil exhibits potent antioxidant activity, while its fatty acid content and other bioactive molecules contribute to its antimicrobial efficacy against various pathogens. The underlying mechanisms of action driving these bioactivities involve intricate molecular interactions, biochemical pathways, and redox processes, which influence microbial cell function and oxidative stress reduction. This review provides a critical analysis of the current mechanistic understanding of palm oil’s biofunctional properties, emphasizing its potential incorporation into engineered biomaterials. Particular focus is given to the chemical composition, reaction pathways, and synergistic potential of palm oil derivatives in material-based formulations. Furthermore, the potential applications of palm oil as a standalone or synergistic agent in novel therapeutic and industrial formulations are explored. By elucidating the mechanistic basis of its bioactivity within material contexts, this review highlights palm oil’s promising role in the development of advanced functional materials for pharmaceutical and dental technologies. Full article

This paper examines how artificial intelligence (AI) can be strategically deployed to enhance urban planning and environmental livability in Riyadh by generating data-driven, people-centric design interventions. Unlike previous studies that concentrate primarily on visualization, this research proposes an integrative appraisal framework that combines AI-generated design with site-specific environmental data and native vegetation typologies. This study was conducted across key jurisdictional areas including the Northern Ring Road, King Abdullah Road, Al Rabwa, Al-Malaz, Al-Suwaidi, Al-Batha, and King Fahd Road. Using AI tools, urban scenarios were developed to incorporate expanded pedestrian pathways (up to 3.5 m), dedicated bicycle lanes (up to 3.0 m), and ecologically adaptive green buffer zones featuring native drought-resistant species such as Date Palm, Acacia, and Sidr. The quantitative analysis of post-intervention outcomes revealed surface temperature reductions of 3.2–4.5 °C and significant improvements in urban esthetics, walkability, and perceived safety—measured on a five-point Likert scale with 80–100% increases in user satisfaction. Species selection was validated for ecological adaptability, minimal maintenance needs, and compatibility with Riyadh’s sandy soils. This study directly supports the Kingdom of Saudi Arabia’s Vision 2030 by demonstrating how emerging technologies like AI can drive smart, sustainable urban transformation. It aligns with Vision 2030’s urban development goals under the Quality-of-Life Program and environmental sustainability pillar, promoting healthier, more connected cities with elevated livability standards. The research not only delivers practical design recommendations for planners seeking to embed sustainability and digital innovation in Saudi urbanism but also addresses real-world constraints such as budgetary limitations and infrastructure integration. Full article