Search Results (772)

Species and phylogenetic diversity play vital roles in sustaining the structure, function, and resilience of plant communities, particularly in tropical rainforests. However, the mechanisms according to which environmental filtering and competitive exclusion influence diversity across forest layers remain insufficiently understood. In this study, we investigated the species and phylogenetic diversity patterns in two representative tropical rainforest sites—Bawangling and Jianfengling—within Hainan Tropical Rainforest National Park, China, focusing on communities associated with the endangered species Hopea hainanensis. We employed a one-way ANOVA and Pearson’s correlation analyses to examine the distribution characteristics and interrelationships among diversity indices and used Mantel tests to assess the correlations with environmental variables. Our results revealed that the plant community in Jianfengling exhibited a significantly higher species richness at the family, genus, and species levels (a total of 288 plant species have been recorded, belonging to 82 families and 183 genera) compared to that in Bawangling (a total of 212 plant species, belonging to 75 families and 162 genera). H. hainanensis held the highest importance value in the middle tree layer across both sites (IV(BWL) = 12.44; IV(JFL) = 5.73), while dominant species varied notably among other forest layers, indicating strong habitat specificity. Diversity indices, including the Simpson index, the Shannon–Wiener index, and Pielou’s evenness, were significantly higher in the large shrub layer of Jianfengling, whereas Bawangling showed a relatively higher Shannon–Wiener index in the middle shrub layer. Phylogenetic diversity (PD) and the phylogenetic structure indices (NRI and NTI) displayed distinct vertical stratification patterns between sites. Furthermore, the PD in Bawangling’s large shrub layer was positively correlated with total phosphorus in the soil, while community evenness was influenced by soil organic carbon and total nitrogen. In Jianfengling, species richness was significantly associated with soil bulk density, altitude, and pH. These findings enhance our understanding of the ecological and evolutionary processes shaping biodiversity in tropical rainforests and highlight the importance of incorporating both species and phylogenetic metrics into the conservation strategies for endangered species such as Hopea hainanensis. Full article

This study explores the revival of cotton (Gossypium spp. L.) farming in Italy through sustainable practices, addressing economic and water-related challenges by integrating cultivar selection, arbuscular mycorrhizal fungi (AMF) inoculation, and deficit irrigation under organic farming. Field trials evaluated two widely grown Mediterranean cultivars (Armonia and ST-318) under three irrigation levels (I-100: 100% crop water requirement; I-70: 70%; I-30: 30%) across two Sicilian soil types (sandy loam vs. clay-rich). Under I-100, lint yields reached 0.99 t ha⁻¹, while severe deficit (I-30) yielded only 0.40 t ha⁻¹. However, moderate deficit (I-70) maintained 75–79% of full yields, proving a viable strategy. AMF inoculation significantly enhanced plant height (68.52 cm vs. 65.85 cm), boll number (+22.1%), and seed yield (+12.5%) (p < 0.001). Cultivar responses differed: Armonia performed better under water stress, while ST-318 thrived with full irrigation. Site 1, with higher organic matter, required 31–38% less water and achieved superior irrigation water productivity (1.43 kg m⁻³). Water stress also shortened phenological stages, allowing earlier harvests—important for avoiding autumn rains. These results highlight the potential of combining adaptive irrigation, resilient cultivars, and AMF to restore sustainable cotton production in the Mediterranean, emphasizing the importance of soil-specific management. Full article

Wild edible mushrooms are increasingly recognised for their nutritional and therapeutic potential, owing to their richness in bioactive compounds and antioxidant properties. This study assessed the chemical composition, antioxidant capacity, and bioaccumulation of heavy metals (Cd, Pb, Ni) in Boletus edulis, Imleria badia, and Leccinum scabrum collected from two forested regions of north-western Poland differing in anthropogenic influence and soil characteristics. The analysis encompassed structural polysaccharides (β- and α-glucans, chitin), carotenoids, L-ascorbic acid, phenolic and organic acids. B. edulis exhibited the highest β-glucan and lycopene contents, but also the greatest cadmium accumulation. I. badia was distinguished by elevated ascorbic and citric acid levels and the strongest DPPH radical scavenging activity, while L. scabrum showed the highest ABTS and FRAP antioxidant capacities and accumulated quinic acid and catechin. Principal component analysis indicated strong correlations between antioxidant activity and phenolic acids, while cadmium levels were inversely associated with antioxidant potential and positively correlated with chitin. Although all metal concentrations remained within EU food safety limits, B. edulis showed consistent cadmium bioaccumulation. From a practical perspective, the results highlight the importance of species selection and sourcing location when considering wild mushrooms for consumption or processing, particularly in the context of nutritional value and contaminant load. Importantly, regular or excessive consumption of B. edulis may result in exceeding the tolerable weekly intake (TWI) levels for cadmium and nickel, which warrants particular attention from a food safety perspective. These findings underscore the influence of species-specific traits and environmental conditions on mushroom biochemical profiles and support their potential as functional foods, provided that metal contents are adequately monitored. Full article

Agriculture is one of the most important sectors of the global economy, but it increasingly faces sustainability challenges in meeting rising food demands. The intensive use of mineral fertilizers not only improves yields, but also causes negative environmental impacts such as increasing greenhouse gas emissions, water eutrophication, and soil degradation. To develop more sustainable solutions, the focus is on organic fertilizers, which are produced using waste and biostimulants such as amino acids. The aim of this study was to develop and characterize liquid nitrogen–calcium–magnesium fertilizers produced by decomposing dolomite with nitric acid followed by further processing and to enrich them with a powdered amino acid concentrate Naturamin-WSP and liquid extracts from digestate, a by-product of biogas production. Nutrient-rich extracts were obtained using water and potassium hydroxide solutions, with the latter proving more effective by yielding a higher organic carbon content (4495 ± 0.52 mg/L) and humic substances, which can improve soil structure. The produced fertilizers demonstrated favourable physical properties, including appropriate viscosity and density, as well as low crystallization temperatures (eutectic points from –3 to –34 °C), which are essential for storage and application in cold climates. These properties were achieved by adjusting the content of nitrogenous compounds and bioactive extracts. The results of the study show that liquid fertilizers enriched with organic matter can be an effective and more environmentally friendly alternative to mineral fertilizers, contributing to the development of the circular economy and sustainable agriculture. Full article

This study, conducted in Mexico, evaluates the agricultural potential of three compost formulations BFS1, BFS2, and BFS3 produced from mining tailings and thermophilic microbial mats and collected from geothermal environments. The physicochemical characterization included pH, electrical conductivity (EC), macronutrients (N, P, K, Ca, Mg, and S), micronutrients (Fe, Zn, B, Cu, Mn, Mo, and Ni), organic matter (OM), and the carbon-to-nitrogen (C/N) ratio. All composts exhibited neutral pH values (7.38–7.52), high OM content (38.5–48.4%), and optimal C/N ratios (10.5–13.9), indicating maturity and chemical stability. Nitrogen ranged from 19 to 21 kg·t⁻¹, while potassium and calcium were present in concentrations beneficial for crop development. However, EC values (3.43–3.66 dS/m) and boron levels (>160 ppm) were moderately high, requiring caution in saline soils or with boron-sensitive crops. A semi-quantitative Compost Quality Index (CQI) ranked BFS3 highest due to elevated OM and potassium content, followed by BFS1. BFS2, while rich in nitrogen, scored lower due to excessive boron. One-way ANOVA revealed no significant difference in nitrogen (p > 0.05), but it did reveal significant differences in potassium (p < 0.01) and boron (p < 0.001) among formulations. These results confirm the potential of mining tailings—microbial mat composts are low-cost, nutrient-rich biofertilizers. They are suitable for field crops or as components in nursery substrates, particularly when EC and boron are managed through dilution. This study promotes the circular reuse of geothermal and industrial residues and contributes to sustainable soil restoration practices in mining-affected regions through innovative composting strategies. Full article

Urban expansion fragments once-contiguous forest patches, generating pronounced edge gradients that modulate soil physicochemical properties and biodiversity. We quantified how fragmentation reshaped the soil microbiome continuum and its implications for soil carbon storage in a temperate urban mixed deciduous forest. A total of 18 plots were considered in this study, with six plots for each fragment type. Intact interior forest (F), internal forest path fragment (IF), and external forest path fragment (EF) soils were sampled at 0–15, 15–30, and 30–45 cm depths and profiled through phospholipid-derived fatty acid (PLFA) chemotyping and amino sugar proxies for living microbiome and microbial-derived necromass assessment, respectively. Carbon fractionation was performed through the chemical oxidation method. Diversity indices (Shannon–Wiener, Pielou evenness, Margalef richness, and Simpson dominance) were calculated based on the determined fatty acids derived from the phospholipid fraction. The microbial biomass ranged from 85.1 to 214.6 nmol g⁻¹ dry soil, with the surface layers of F exhibiting the highest values (p < 0.01). Shannon diversity declined systematically from F > IF > EF. The microbial necromass varied from 11.3 to 23.2 g⋅kg⁻¹. Fragmentation intensified the stratification of carbon pools, with organic carbon decreasing by approximately 14% from F to EF. Our results show that EFs possess a declining microbiome continuum that weakens their carbon sequestration capacity in urban forests. Full article

Organic farming is widely recognized as a promising practice for sustainable agriculture, yet its long-term ecological impacts remain insufficiently investigated. In this study, we evaluated these impacts by comparing heavy metal concentrations, soil invertebrate communities, and microbial profiles between long-term organic and conventional farming systems. A comparative analysis was conducted on 24 plot soils from two paired organic and conventional farm systems in Beijing, each managed continuously for over 20 years. Our results revealed that soils under organic management consistently contained 10.8% to 73.7% lower heavy metals, along with reduced geo-accumulation indices (I_geo, a standardized metric for soil contamination assessment), indicating decreased contamination risks. In terms of soil fauna, while conventional soils showed higher Collembola abundance, organic farming significantly enhanced Collembola richness and diversity by 20.6% to 55.0%. Microbial sequencing likewise revealed enhanced richness and diversity of bacteria and fungi in organic soils. These microbial communities also displayed shifts in dominant taxa and more stable co-occurrence networks under organic management. Principal component analysis and Mantel tests identified soil pH and nutrients as key drivers of soil biodiversity, while heavy metals also imposed negative influences. Collectively, these findings demonstrate that long-term organic farming not only mitigates environmental risks associated with soil contaminants but also promotes belowground ecological integrity by supporting biodiversity of soil fauna and microbiota. This study highlights the ecological significance of sustained organic practices and provides critical insights for advancing sustainable agricultural developments. Full article

Desert plant communities play a vital role in sustaining the stability of arid ecosystems; however, they demonstrate limited resilience to environmental changes. A critical aspect of understanding community assembly mechanisms is determining whether soil texture heterogeneity affects vegetation diversity in arid deserts, especially under conditions of extreme water scarcity and restricted nutrient availability. This study systematically examined the relationships between plant diversity and soil physicochemical properties across four soil texture types—sand, sandy loam, loamy sand, and silty loam—by selecting four representative desert systems in the Hami region of Xinjiang, China. The objective was to elucidate the mechanisms through which soil texture may impact desert plant species diversity. The findings revealed that silty loam exhibited distinct characteristics in comparison to the other three sandy soil types. Despite its higher nutrient content, silty loam demonstrated the lowest vegetation diversity. The Shannon–Wiener index (H′), Simpson dominance index (C), Margalef richness index (D), and Pielou evenness index (Jsw) for silty loam were all lower compared to those for sand, sandy loam, and loamy sand. However, silty loam exhibited higher values in electrical conductivity (EC), urease activity (SUR), and nutrient content, including soil organic matter (SOM), ammonium nitrogen (NH⁴⁺-N), and available potassium (AK), than the other three soil textures. This study underscores the significant regulatory influence of soil texture on plant diversity in arid environments, offering new insights and practical foundations for the conservation and management of desert ecosystems. Full article

Semi-natural grasslands are some of the most species-rich habitats in Europe and provide important ecosystem services such as biodiversity conservation, carbon sequestration and soil fertility maintenance. This study investigates how different intensities of grassland management affect the composition of functional groups and soil chemical properties. Five grassland management systems were analyzed: Cut3—three cuts per year; LGI—low grazing intensity; CG—combined cutting and grazing; Cut4—four cuts per year; and HGI—high grazing intensity. The functional groups assessed were grasses, legumes and forbs, while soil samples from three depths (0–10, 10–20 and 20–30 cm) were analyzed for their chemical properties (soil organic carbon—SOC; soil total nitrogen—STN; inorganic soil carbon—SIC; soil organic matter—SOM; potassium oxide—K₂O; phosphorus pentoxide—P₂O₅; C/N ratio; and pH) and physical properties (volumetric soil water content—VWC; bulk density—BD; and porosity—POR). The results showed that less intensive systems had a higher proportion of legumes, while species diversity, as measured via the Shannon index, was the highest in the Cut4 system. The CG system tended to have the highest SOC and STN at a 0–10 cm depth, with a similar trend observed for SOC_stock at a 0–30 cm depth. The Cut4, HGI and CG systems also had an increased STN_stock. Both grazing systems had the highest P₂O₅ content. A tendency towards a higher BD was observed in the top 10 cm of soil in the more intensive systems. Choosing a management strategy that is tailored to local climate and site conditions is crucial for maintaining grassland stability, enhancing carbon sequestration and promoting long-term sustainability in the context of climate change. Full article

Protecting the soil ecosystem’s functioning is one of the main goals of recent regulations of chemicals. It is important to take soil biodiversity into account when designing cropping systems and measuring their impacts. Our main objective was to evaluate the effects of an organic amendment on soil nematode biodiversity compared to two years of fumigation. The plot-trial was conducted on tomato and lettuce plants under greenhouse, and free-living nematodes were used as bio-indicators of soil health. Treatments included a soil fumigant (applied once or twice over time), water control, and an organic substance. Soil samplings were carried out to determine the Meloidogyne incognita reproduction factor and the soil nematode community analysis using soil biological indicators. Data showed that soil fumigation clearly made the soil increasingly dependent on chemicals. Furthermore, fumigants suppressed pests and pathogens as well as their natural antagonists, causing a lack of biodiversity. While soils treated with organic matter respond slowly to stressors, they are progressively more suppressive thanks to biodiversity enrichment. Nematodes have proven to be useful indicators of the soil biota in response to biotic or abiotic disturbances. Their species richness and functional diversity make them valid bioindicators of soil management impact. Full article

The use of mineral fertilisers has increased in recent years, but this has had a negative effect on the environment, including causing the water in rivers and lakes to become too rich in nutrients, a process known as eutrophication. Current research focuses on producing fertiliser materials that are environmentally friendly. The aim of this study was to examine the impact of novel specialised organic-mineral fertilisers (OMFs: NP 24-12, NP 10-10, and NP 10-10 Zn⁺) on the yield and chemical composition of maize. These fertilisers were compared with a control (no fertiliser) and with other fertilisers (mixture of commercial fertilisers (MCFs): NP 24-12 and NP 10-10) that were used as a reference. All fertilisers increased the SPAD index at the fifth leaf unfolded stage of maize, with the majority (apart from OMF NP 10-10) also increasing it at the panicle emergence stage. MCF NP 10-10 had the most positive effect on the plant height, while OMF NP 10-10 had the least positive effect. All fertilisers had a positive effect on maize growth and development, with MCFs NP 10-10 and NP 24-12 having by far the strongest effect on increasing crop yields. The yield of plants fertilised with OMFs NP 24-12, NP 10-10, and NP 10-10 Zn⁺ was lower than the yields of plants fertilised with MCF NP 24-12 and MCF NP 10-10. OMF NP 10-10 caused a greater increase in the contents of all elements, and OMF NP 24-12 caused a greater increase in most elements (except P and Ca) in maize than MCFs did at an identical NP ratio. OMF NP 10-10 Zn⁺ was found to have a significant impact on the mineral composition of maize, resulting in a decline in Ca and P levels, along with a notable increase in Mg, Zn, and Cu concentrations. The most significant differences were observed for Cu and Zn. The OMFs, notably NP 24-12 and NP 10-10, exhibited a comparatively diminished acidifying impact in comparison with the MCFs. The application of fertilisers resulted in a significant increase in soil nutrient levels, with most fertilisers increasing the soil N, P, and Zn contents. Additionally, the OMFs led to an increase in Cu. However, MCFs NP 24-12 and NP 10-10 reduced the soil Cu and Mn contents. Studies should include other species as they have different needs. Field experiments are also needed. Full article

(1) Background: The escalating issue of soil degradation caused by excessive chemical fertilizer application poses significant threats to the sustainable development of Chinese flowering cabbage (Brassica campestris L. ssp. chinensis (L.) var. utilis Tsen et Lee) production. This research aimed to identify the impacts of reduced chemical fertilizer application integrated with organic amendments on cabbage yield and rhizosphere soil microenvironment characteristics. (2) Methods: A biennial field experiment was conducted during the 2022–2023 growing seasons at Lijun Town, Yinchuan City, Ningxia Hui Autonomous Region. Five treatments were tested: (i) Control (CK, no fertilizer); (ii) Conventional chemical fertilization (CF1, chemical fertilizer only); (iii) Reduced chemical fertilization (CF2, 30% less chemical fertilizer); (iv) CF2 + Well-decomposed chicken manure (FCM, 30% less chemical fertilizer + rotted chicken manure); and (v) CF2 + Vermicompost (FEM, 30% less chemical fertilizer + vermicompost). (3) Results: In 2023, the FCM treatment reduced electrical conductivity (EC) by 24.80% and pH by 2.16%, while the FEM treatment decreased EC by 31.13% and pH by 3.84% compared to controls. The FEM treatment significantly enhanced total nitrogen content by 12.71% and 8.85% relative to CF1 and FCM treatments, respectively. Compared to CF1, FEM increased soil organic matter content by 10.49% in 2022 and 11.24% in 2023. Organic fertilizer amendments elevated available nitrogen, phosphorus, and potassium levels while enhancing sucrase activity: FCM and FEM treatments increased sucrase activity by 23.62% and 32.00%, respectively, in 2022. Organic fertilization improved bacterial diversity and richness, optimized microbial community structure, and increased the relative abundance of Bacillus. It also upregulated microbial metabolic pathways related to carbohydrate and amino acid metabolism. Soil nutrients and bacterial community structure showed positive correlations with yield, whereas soil enzyme activities exhibited negative correlations. Key factors influencing yield were identified as Proteobacteria, Chloroflexi, available potassium, organic matter, available nitrogen, Actinobacteria, Firmicutes, total nitrogen, pH, and sucrase activity. (4) Conclusions: Integrated analysis of yield and soil microenvironmental parameters demonstrates that the fertilization regimen combining 30% chemical fertilizer reduction with vermicompost amendment (FEM) constitutes a more efficient fertilization strategy for Chinese flowering cabbage, making it suitable for regional promotion in the Ningxia area. Full article

Soil erosion-prone areas require effective microbial treatments to improve soil bacterial communities and functional traits. Understanding the driving effects of different microbial interventions on soil ecology is essential for restoration efforts. Single and combined microbial treatments were applied to soil. Bacterial community structure was analyzed via 16S IRNA high-throughput sequencing, and functional groups were predicted using FAPROTAX. Soil microbial carbon, nitrogen, metabolic entropy, and enzymatic activity were assessed. Microbial Carbon and Metabolic Activity: The Arbuscular mycorrhizal fungi (AMF) and Bacillus mucilaginosus (BM) (AMF.BM) treatment exhibited the highest microbial carbon content and the lowest metabolic entropy. The microbial carbon-to-nitrogen ratio ranged from 1.27 to 3.69 across all treatments. Bacterial Community Composition: The dominant bacterial phyla included Firmicutes, Proteobacteria, Acidobacteria, Bacteroidetes, and Actinobacteria. Diversity and Richness: The AMF and Trichoderma harzianum (TH) (AMF.TH) treatment significantly reduced diversity, richness, and phylogenetic diversity indices, while the AMF.BM treatment showed a significantly higher richness index (p < 0.05). Relative Abundance of Firmicutes: Compared to the control, the AMF, TH.BM, and TH treatments decreased the relative abundance of Firmicutes, whereas the AMF.TH treatment increased their relative abundance. Environmental Correlations: Redundancy and correlation analyses revealed significant correlations between soil organic matter, magnesium content, and sucrase activity and several major bacterial genera. Functional Prediction: The AMF.BM treatment enhanced the relative abundance and evenness of bacterial ecological functions, primarily driving nitrification, aerobic ammonia oxidation, and ureolysis. Microbial treatments differentially influence soil bacterial communities and functions. The AMF.BM combination shows the greatest potential for ecological restoration in erosion-prone soils. Full article

Biochar and seaweed fertilizers could improve soil quality and promote plant growth. However, the key soil factors and microbial mechanisms that drive maize growth in coastal saline–alkali soils remain unclear. A soil culture experiment was designed with four treatments—no organic fertilizer (CK), single seaweed fertilizer (F), single biochar (B), and combined application of seaweed fertilizer and biochar (BF)—to investigate the effects of biochar and seaweed fertilizer on maize growth and its mechanism. The results showed that B and BF significantly increased maize aboveground biomass by 8.86% and 17.28% compared to CK, respectively. The soil organic carbon, total nitrogen, available nitrogen, available phosphorus, available potassium content, and pH of B and BF were significantly increased. Bacterial diversity increased under B and BF, while fungal richness decreased under BF. The changes in the fungal community were mainly affected by soil available nitrogen, but there was no significant correlation between bacterial communities and these indicators. Pearson correlation analysis suggested that the bacterial Chao1 index was significantly positively correlated with maize growth indicators, soil available phosphorus, and available potassium, as well as the bacterial PD whole tree index with leaf area and available phosphorus. The fungal Shannon index was significantly negatively correlated with maize plant height, leaf area, SPAD, aboveground biomass, and soil total nitrogen and available nutrients. Overall, biochar and seaweed fertilization could significantly promote maize growth by improving soil chemical properties and microbial communities in coastal saline–alkali soils. Full article

The Mediterranean region is increasingly confronted with intersecting environmental, agricultural, and socio-economic challenges, including biowaste accumulation, soil degradation, and high dependency on imported fossil fuels. Biomethane, a renewable substitute for natural gas, offers a strategic solution that aligns with the region’s need for sustainable energy transition and circular resource management. This review examines the current state of biomethane production in the Mediterranean area, with a focus on anaerobic digestion (AD) technologies, feedstock availability, policy drivers, and integration into the circular bioeconomy (CBE) framework. Emphasis is placed on the valorisation of regionally abundant feedstocks such as olive pomace, citrus peel, grape marc, cactus pear (Opuntia ficus-indica) residues, livestock manure, and the Organic Fraction of Municipal Solid Waste (OFMSW). The multifunctionality of AD—producing renewable energy and nutrient-rich digestate—is highlighted for its dual role in reducing greenhouse gas (GHG) emissions and restoring soil health, especially in areas threatened by desertification such as Sicily (Italy), Spain, Malta, and Greece. The review also explores emerging innovations in biogas upgrading, nutrient recovery, and digital monitoring, along with the role of Renewable Energy Directive III (RED III) and national biomethane strategies in scaling up deployment. Case studies and decentralised implementation models underscore the socio-technical feasibility of biomethane systems across rural and insular territories. Despite significant potential, barriers such as feedstock variability, infrastructural gaps, and policy fragmentation remain. The paper concludes with a roadmap for research and policy to advance biomethane as a pillar of Mediterranean climate resilience, energy autonomy and sustainable agriculture within a circular bioeconomy paradigm. Full article