Search Results (217)

Background: Ingestion of dietary fibers (DFs) is a safe and accessible intervention associated with reductions in blood pressure (BP) and cardiovascular mortality. However, the mechanisms underlying the antihypertensive effects of DFs remain poorly defined. This systematic review and meta-analysis evaluates how DFs influence BP regulation by modulating gut microbial composition and enhancing short-chain fatty acid (SCFA) production. Methods: MEDLINE and EMBASE were systematically searched for interventional studies published between January 2014 and December 2024. Eligible studies assessed the effects of DFs or other prebiotics on systolic BP (SBP) and diastolic BP (DBP) in addition to changes in gut microbial or SCFA composition. Results: Of the 3010 records screened, nineteen studies met the inclusion criteria (seven human, twelve animal). A random-effects meta-analysis was conducted on six human trials reporting post-intervention BP values. Prebiotics were the primary intervention. In hypertensive cohorts, prebiotics significantly reduced SBP (−8.5 mmHg; 95% CI: −13.9, −3.1) and DBP (−5.2 mmHg; 95% CI: −8.5, −2.0). A pooled analysis of hypertensive and non-hypertensive patients showed non-significant reductions in SBP (−4.5 mmHg; 95% CI: −9.3, 0.3) and DBP (−2.5 mmHg; 95% CI: −5.4, 0.4). Animal studies consistently showed BP-lowering effects across diverse etiologies. Prebiotic interventions restored bacterial genera known to metabolize DFs to SCFAs (e.g., Bifidobacteria, Akkermansia, and Coprococcus) and increased SCFA levels. Mechanistically, SCFAs act along gut–organ axes to modulate immune, vascular, and neurohormonal pathways involved in BP regulation. Conclusions: Prebiotic supplementation is a promising strategy to reestablish BP homeostasis in hypertensive patients. Benefits are likely mediated through modulation of the gut microbiota and enhanced SCFA production. Full article

Recent findings highlight that Reelin, a glycoprotein involved in neural development, synaptic plasticity, and neuroinflammation, plays some specific roles in neurodegenerative disorders associated with aging, such as age-related macular degeneration (AMD) and Alzheimer’s disease (AD). Reelin modulates synaptic function and guarantees homeostasis in neuronal-associated organs/tissues (brain and retina). The expression of Reelin is dysregulated in these neurological disorders, showing common pathways depending on chronic neurogenic inflammation and/or dysregulation of the extracellular matrix in which Reelin plays outstanding roles. Recently, the relationship between AMD and AD has gained increasing attention as they share many common risk factors (aging, genetic/epigenetic background, smoking, and malnutrition) and histopathological lesions, supporting certain pathophysiological crosstalk between these two diseases, especially regarding neuroinflammation, oxidative stress, and vascular complications. Outside the nervous system, Reelin is largely produced at the gastrointestinal epithelial level, in close association with innervated regions. The expression of Reelin receptors inside the gut suggests interesting aspects in the field of the gut–brain–eye axis, as dysregulation of the intestinal microbiota has been frequently described in neurodegenerative and behavioral disorders (AD, autism, and anxiety and/or depression), most probably linked to inflammatory, neurogenic mediators, including Reelin. Herein we examined previous and recent findings on Reelin and neurodegenerative disorders, offering findings on Reelin’s potential relation with the gut–brain and gut–brain–eye axes and providing novel attractive hypotheses on the gut–brain–eye link through neuromodulator and microbiota interplay. Neurodegenerative disorders will represent the ground for a future starting point for linking the common neurodegenerative biomarkers (β-amyloid and tau) and the new proteins probably engaged in counteracting neurodegeneration and synaptic loss. Full article

Egg yolk immunoglobulin Y (IgY) possesses advantages such as low cost, easy availability, simple preparation, high antigen specificity, absence of drug residues, and compliance with animal welfare standards, making it an environmentally friendly and safe alternative to antibiotics. This research utilizes IgY antibody technology to develop a multivalent passive immune vaccine for major pathogenic bacteria in aquaculture. In this study, IgY antibodies against live Shewanella xiamenensis (LSX-IgY) and inactivated S. xiamenensis (ISX-IgY) were prepared by immunizing laying hens, and passive immunization protection experiments were conducted in Carassius auratus infected with S. xiamenensis and Aeromonas hydrophila. The passive immunization protection rates of LSX-IgY and ISX-IgY against S. xiamenensis were 63.64% and 72.73%, respectively, and the passive cross-protection rates against A. hydrophila were 50% and 71.43%, respectively. Further, C. auratus sera could specifically bind to S. xiamenensis or A. hydrophila in vitro, and the phagocytic activity of leukocytes was increased. LSX-IgY and ISX-IgY could reduce the bacterial load in the C. auratus kidneys. Meanwhile, they could significantly reduce the levels of antioxidant factors in serum and inhibit the mRNA expression of inflammation-related factors in the kidneys and spleens. Additionally, histopathology and immunofluorescence analysis showed that both IgY preparations preserved tissue integrity and reduced the expression of apoptosis factor (p53) and DNA damage factor (γH2A.X) of visceral organs, respectively. In summary, LSX-IgY and ISX-IgY can combat various bacterial infections, with no significant difference between the two. Additionally, inactivated bacterial immunization is more aligned with animal welfare standards for laying hens. Therefore, ISX-IgY is expected to serve as a multivalent vaccine against major aquaculture pathogens. Full article

The global port and maritime industry is experiencing a new paradigm shift known as the artificial intelligence transformation (AX). Thus, domestic container-terminal companies should focus beyond mere automation to a paradigm shift in AI that encompasses operational strategy, organizational structure, system, and human resource management. This study proposes a resilience-based AX strategy and implementation system that allows domestic container-terminal companies to proactively respond to the upcoming changes in the global supply chain, thus securing sustainable competitiveness. In particular, we aim to design an AI-based governance model to establish a trust-based logistics supply chain (trust value chain). As a research method, the core risk factors of AX processes were scientifically identified via text-mining and fault-tree analysis, and a step-by-step execution strategy was established by applying a backcasting technique based on scenario planning. Additionally, by integrating social control theory with new governance theory, we designed a flexible, adaptable, and resilience-oriented AI governance system. The results of this study suggest that the AI paradigm shift should be promoted by enhancing the risk resilience, trust, and recovery of organizations. By suggesting AX strategies and policy as well as institutional improvement directions that embed resilience to secure the sustainable competitiveness of AI-based smart ports in Korea, this study serves as a basis for establishing strategies for the domestic container-terminal industry and for constructing a global leading model. Full article

Urbanisation and land-use change are among the main pressures on soil health in periurban areas, but the multifunctionality of grassland soils is still not sufficiently recognised. In this study, the physical and chemical properties of soils under grassland, forest and croplands in the periurban area of Zagreb were investigated in a two-year period. Grasslands consistently exhibited multifunctional benefits, including high organic matter content (4.68% vs. 2.24% in cropland), improved bulk density (1.14 vs. 1.24 g cm⁻³) and an active carbon cycle indicated by increased CO₂ emissions (up to 1403 kg ha⁻¹ day⁻¹ in 2021). Forest soils showed the highest aggregate stability (91.4%) and infiltration (0.0006 cm s⁻¹), while croplands showed signs of structural degradation with the highest bulk density and lowest water retention (39.9%). Temporal variation showed that grassland was particularly responsive to favourable climatic conditions, with soil porosity and water content improving yearly. Principal component analysis showed that soil structure, biological activity and moisture regulation were linked, with grassland plots favourably positioned along the axes of resilience. The absence of tillage and the presence of permanent vegetation cover contributed to their high capacity for climate and water regulation and carbon sequestration. These results emphasise the importance of protecting and managing grasslands as an important component of urban green areas. Practices such as mulching, minimal disturbance and continuous cover can maximise the ecosystem services of grassland soils. In addition, the results highlight the potential risk of trace metal accumulation in cropland and grassland soils located near urban and farming infrastructure, underlining the need for regular monitoring in periurban environments. Integrating grassland functions into urban planning and policy is essential for improving the sustainability and resilience of periurban landscapes. Full article

This study proposes a conceptual model that explains the influence of leadership on the relationship between human intelligence (HI) and artificial intelligence (AI). A qualitative, non-systematic literature review was conducted in Scopus and Web of Science of the literature published in the last 5 years, using Boolean combinations of the terms “leadership,” “artificial intelligence,” and “human intelligence.” The thematic analysis allowed the identification of conceptual patterns and research gaps; the model elaborated from the review shows that leadership has an ethical and strategic mediation in the HI-AI relationship in a hybrid space of cooperation, in which automated decisions are put in real context through human judgment and reasoning; ethical governance mechanisms emerge for systems supported by artificial intelligence; and finally, a balancing mechanism to algorithmic efficiency is established through cognitive adaptability. The proposed framework offers organizations some guidelines for human supervision processes for AI-supported systems that integrate ethical evaluations into automated processes. It proposes elements—leadership tools that enhance the relationship between human intelligence and artificial intelligence. This article contributes to the management of organizations by proposing a model that recognizes leadership as a dynamic facilitator between HI and AI, integrating transdisciplinary knowledge of management, technological ethics, and cognitive science, and proposing an ethical interrelationship in the decision-making architectures between HI and AI. The proposed model establishes leadership mediation of human–AI interaction through four axes showing how leadership acts as the axis that brings together human and technological systems to work together. Hierarchical interaction creates a hybrid interaction that is highly flexible, efficient, and has ethical oversight. Finally, the proposed model is an open system that interacts with the environment and is understood as a flexible tool to support strategic decision-making in complex environments. Full article

Stem-cell behavior is governed not solely by intrinsic genetic programs but by highly specialized microenvironments—or niches—that integrate structural, biochemical, and mechanical cues to regulate quiescence, self-renewal, and differentiation. This review traces the evolution of stem-cell niche biology from foundational embryological discoveries to its current role as a central determinant in tissue regeneration and disease. We describe the cellular and extracellular matrix architectures that define adult stem-cell niches across diverse organs and dissect conserved signaling axes—including Wnt, BMP, and Notch—that orchestrate lineage commitment. Emphasis is placed on how aging, inflammation, fibrosis, and metabolic stress disrupt niche function, converting supportive environments into autonomous drivers of pathology. We then examine emerging therapeutic strategies that shift the regenerative paradigm from a stem-cell-centric to a niche-centric model. These include stromal targeting (e.g., FAP inhibition), which are engineered scaffolds that replicate native niche mechanics, extracellular vesicles that deliver paracrine cues, and composite constructs that preserve endogenous cell–matrix interactions. Particular attention is given to cardiac, hematopoietic, reproductive, and neurogenic niches, where clinical failures often reflect niche misalignment rather than intrinsic stem-cell deficits. We argue that successful regenerative interventions must treat stem cells and their microenvironment as an inseparable therapeutic unit. Future advances will depend on high-resolution niche mapping, mechanobiologically informed scaffold design, and niche-targeted clinical trials. Re-programming pathological niches may unlock regenerative outcomes that surpass classical cell therapies, marking a new era of microenvironmentally integrated medicine. Full article

Plastic overconsumption has emerged as a major environmental pollutant, with degraded micro- and nanoplastic (MNP) particles being consumed by a vast variety of species. MNPs, particles < 5 mm, contain endocrine-disrupting chemicals (EDCs), which can bind to hormone receptors and disrupt the proper endocrinological function of a variety of organs. This review explores the toxicological impact of MNPs on the hypothalamus, pituitary gland, thyroid, pineal body, ovaries, and testes, as well as the effects of the endocrinological regulatory axes, including the hypothalamic–pituitary–gonadal (HPG), hypothalamic–pituitary–thyroid (HPT), and hypothalamic–pituitary–adrenal (HPA) axes. The disruption of these hormonal feedback systems leads to reproductive dysfunction, neurotoxicity, cytotoxicity, immunotoxicity, and metabolic disorders. The gonads are particularly susceptible, with studies demonstrating oxidative stress, cellular apoptosis, and infertility due to MNP exposure. Given the widespread presence of MNPs and their impact on human health, further research is critical to understand their long-term effects and develop strategies to reduce exposure. Full article

In this work, the authors compared microCT and in vivo ultrasonography in terms of accuracy and efficacy for measuring the volume of various organs in mice. Two quantification protocols were applied: ellipsoidal volume measuring maximum diameters in all three axes in both imaging systems and manual delineation of organ borders in microCT studies. The results were compared with ex vivo volumes. In general, both imaging techniques and quantification protocols are accurate, but ultrasound is faster in both acquisition and analysis. The only accurate method for heart volume measurement is manual segmentation on microCT. For the ovary, none of the techniques and protocols had a positive correlation with ex vivo volume. The three-diameter method can be used for ellipsoid organs because of its rapidity, but for more irregular structures, manual segmentation is recommended, although it is time-consuming. Full article

In recent years, it has become clear that non-coding RNAs play an important role in regulating the development of various organs and pathological conditions, including cerebral ischemia and reperfusion. Non-coding RNAs are mainly represented by long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and circular RNAs (circRNAs). Most of the human genome is transcribed into such RNAs. Excessive activation of autophagy during cerebral ischemia and reperfusion results in autophagic neuronal death in addition to apoptotic death. This review shows that regulation occurs via the lncRNA (or circRNA)/miRNA/target protein signaling axes. A knockdown or a decrease in lncRNA level can lead to a significant increase in miRNA levels, followed by a decrease in the levels of messenger RNA (mRNA) of autophagy-related protein (ATG) and ATG protein itself. This leads to inhibition of autophagy and alleviation of brain ischemia–reperfusion injury. Changes in miRNA and mRNA levels of the target protein occur due to the presence of complementary nucleotide sequences with lncRNA and miRNA, respectively. If the target protein is not an ATG protein, neuroprotection during cerebral ischemia and reperfusion can result from both inhibition and activation of autophagy. The further study of the regulatory role of non-coding RNAs is important as it may help to counteract the effects of excessive autophagy activation and other adverse effects of ischemia–reperfusion injury Full article

Background/Objectives: In 4-octylphenol (4-OP), a toxic environmental pollutant with endocrine disruptive effect, the use of 4-OP causes pollution in the freshwater environment and poses risks to aquatic organisms. Common carps (Cyprinus carpio L.) live in freshwater and are experimental animals for studying the toxic effects of environmental pollutants on fish. Its heart is susceptible to toxicants. However, whether 4-OP has a toxic effect on common carp heart remains unknown. Methods: Here, we conducted a common carp 4-OP exposure experiment (carp treated with 17 μg/L 4-OP for 45 days), aiming to investigate whether 4-OP has a toxic effect on common carp hearts. We observed the microstructure and ultrastructure of carp heart and detected autophagy genes, mitochondrial fission genes, mitochondrial fusion genes, glycolytic enzymes, AMPK, ATPase, and oxidative stress factors, to investigate the molecular mechanism of 4-OP induced damage in common carp hearts. Results: Our results showed that 4-OP exposure caused mitochondrial damage, autophagy, and damage in common carp hearts. 4-OP exposure increased the levels of miR-144, and eight autophagy factors (Beclin1, RB1CC1, ULK1, LC3-I, LC3-II, ATG5, ATG12, and ATG13), and decreased the levels of four autophagy factors (PI3K, AKT, mTOR, and SQSTM1). Furthermore, 4-OP exposure induced the imbalance between mitochondrial fission and fusion and mitochondrial dynamics imbalance, as demonstrated by the increase in three mitochondrial fission factors (Mff, Drp1, and Fis1) and the decrease in three mitochondrial fusion factors (Mfn1, Mfn2, and Opa1). Moreover, excess 4-OP treatment caused energy metabolism disorder, as demonstrated by the reduction in four ATPase (Na⁺K⁺-ATPase, Ca²⁺Mg²⁺-ATPase, Ca²⁺-ATPase, and Mg²⁺-ATPase), elevation in four glycolysis genes (HK1, HK2, LDHA, and PGK1), reduction in glycolysis gen (PGAM2), and the elevation in energy-sensing AMPK. Finally, 4-OP treatment induced the imbalance between antioxidant and oxidant and oxidative stress, as demonstrated by the increase in oxidant H₂O₂, and the decreases in five antioxidant factors (CAT, SOD, T-AOC, Nrf2, and HO-1). Conclusions: miR-144 mediated autophagy by targeting PI3K, mTOR, and SQSTM1, and the miR-144/PI3K-AKT-mTOR/ULK1 pathway was involved in 4-OP-induced autophagy. Mff-Drp1 axis took part in 4-OP-caused mitochondrial dynamics imbalance, and mitochondrial dynamics imbalance mediated autophagy via Mfn2-SQSTM1, Mfn2/Beclin1, and Mff-LC3-II axes. Energy metabolism disorder mediated mitochondrial dynamics imbalance through the AMPK-Mff-Drp1 pathway. Oxidative stress mediated energy metabolism disorder via the H₂O₂-AMPK axis. Taken together, oxidative stress triggered energy metabolism disorder, induced mitochondrial dynamics imbalance, and caused autophagy via the H₂O₂-AMPK-Mff-LC3-II pathway. Our study provided references for the toxic effects of endocrine disruptor on common carp hearts, and provided a basis for assessing environmental pollutant-induced damage in common carp heart. We only studied the toxic effects of 4-OP on common carp, and the toxic effects of 4-OP on other fish species need to be further studied. Full article

Ammonia (NH₃) is a promising carbon-free marine fuel that is aligned with the International Maritime Organization’s (IMO) decarbonization targets. However, its high toxicity and flammability pose serious explosion hazards, particularly in confined fuel preparation spaces. This study investigates the influence of the ignition source location on the explosion characteristics of ammonia within an ammonia fuel preparation room using computational fluid dynamics (CFD) simulations via the FLACS platform. Nineteen ignition scenarios are established along the X-, Y-, and Z-axes. Key parameters, such as the maximum overpressure, pressure rise rate, reduction rate of flammable gas, ignition detection time, and spatial–temporal distributions of temperature and combustion products, are evaluated. The results show that the ignition location plays a critical role in the explosion dynamics. Ceiling-level ignition (Case 19) produced the highest overpressure (4.27 bar) and fastest pressure rise rate (2.20 bar/s), indicating the most hazardous condition. In contrast, the forward wall ignition (Case 13) resulted in the lowest overpressure (3.24 bar) and limited flame propagation. These findings provide essential insights into the risk assessment and safety design of ammonia-fueled marine systems. Full article

Between 2017 and 2023, three sub-regional spatial plans were developed for specific areas of the Valencian Autonomous Region: the Alicante–Elche Metropolitan Area, Vega Baja del Segura County, and the Central Valencian Counties. Their main aim was to develop an envisaged territorial model as a binding framework for municipal urban master plans. During the elaboration of these plans, a set of activities was developed to understand the main consensus among stakeholders. The main axes of the planning process were addressed during territorial working groups conducted with relevant stakeholders, including those focused on green infrastructure, settlement systems, and infrastructure systems. Participants were selected from the public administration, non-governmental organizations, the economic sector, and the university. Drawing on the outcomes of the participatory activities, various factors are analyzed, including the ratio between invited stakeholders and actual participants in the territorial workshops, the contributions made by participants in each main axis of the plan, the inputs provided according to stakeholder type, the nature of these contributions, and the degree of alignment between the inputs and the objectives of the PAT. The present study reveals how contextual factors can influence the orientation of the participatory process. At times, contingency may emerge as an opportunity to energize a governance process. Similarly, the participatory technique is validated for its potential to enrich the process, while also highlighting the absence of voices not aligned with spatial planning in the participatory settings. Full article

Fermentation is one of the oldest food processing techniques and is widely utilized in dairy product processing, during which nutrient availability and bioactive compounds are altered. However, the complete mode of action by which fermented dairy exerts beneficial effects on the host remains unknown. The present study investigated the effect of milk and yogurt ingestion alone or combined with prebiotic inulin on the transcriptome of colonic mucosa, liver, and femur in healthy rats. Young growing male rats were fed one of four experimental diets containing (1) skimmed milk, (2) skimmed milk supplemented with inulin (5% w/w), (3) yogurt, or (4) yogurt supplemented with inulin (5% w/w) for 6 weeks. Microarray results revealed that yogurt consumption resulted in 2195 upregulated differential expressed genes (DEGs) and 1474 downregulated DEGs in colonic mucosa as compared with milk consumption. According to Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, tight junction-, immune system-related pathways in the colonic mucosa and metabolic pathways in the liver were enriched with yogurt consumption. No evident differences were identified in the bone transcriptome between the diet groups. In conclusion, the study found that the intake of fermented dairy exerts more pronounced effects on gene expression in the intestinal tissue than prebiotics supplementation. Full article

Vaccination is widely recognized as an effective strategy for preventing various bacterial and viral diseases. In this study, protein, DNA, and egg yolk antibody (IgY) vaccines targeting the outer membrane protein VF14355 of Vibrio fluvialis (V. fluvialis) were administered to goldfish (Carassius auratus, C. auratus) subsequently challenged with V. fluvialis and Aeromonas hydrophila (A. hydrophila). The immune efficacy of the three VF14355 vaccines was evaluated through their immune activities, protective rates, anti-inflammatory and antioxidant effects, histopathology, and immunofluorescence, and the results indicated that the protective rates in the three immunized groups were significantly higher than those in the control group; furthermore, the number of kidney bacteria was significantly reduced in the immunized group compared to the control group. The ELISA results demonstrated an in vitro interaction between the bacteria and C. auratus serum. The plasma phagocytosis index and phagocytosis percentage were significantly increased in C. auratus, and their serum immune factor levels, including those of acid phosphatase (ACP), alkaline phosphatase (AKP), and lysozyme (LZM), were increased, while those of serum antioxidant factors, such as superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA), were reduced in the immunized group; notably, the expression levels of inflammatory factors were also diminished in the immunized groups. Histopathological analyses further revealed that the organ structures of the immunized group remained intact, and immunofluorescence tests indicated significant reductions in apoptosis factor p53 and DNA damage factor γH2A.X in kidney tissues. Therefore, the protein, DNA, and IgY vaccines of VF14355 demonstrate the potential to confer resistance against various bacterial infections, positioning them as promising multivalent vaccine candidates for aquaculture. Full article