Search Results (3,703)

Musa haekkinenii is a compact wild banana species with emerging value in ornamental horticulture, yet its adaptive responses to environmental factors remain underexplored. This study investigated the morpho-physiological and anatomical responses of M. haekkinenii to contrasting light regimes and irrigation water qualities to identify optimal cultivation conditions. A 210-day factorial experiment was conducted under subtropical greenhouse conditions using a split-plot design, with light intensity (full sun vs. shade) and irrigation water quality (reverse osmosis vs. well water) as treatment factors. Plants grown under shaded conditions and irrigated with reverse osmosis water exhibited significant increases in plant height, pseudostem diameter, leaf number, and sucker production, alongside enhanced pigment accumulation and photosynthetic performance. In contrast, full-sun plants irrigated with well water showed reduced growth, lower photosynthetic efficiency, and increased substrate salinity, indicating additive effects of light and osmotic stress. Leaf anatomical analysis revealed greater stomatal size and density under shade, particularly when combined with high-quality irrigation. Multivariate analysis further supported the association of favorable trait expression with shaded conditions and reverse osmosis water. These findings highlight the importance of microenvironmental management in enhancing the physiological stability and ornamental quality of M. haekkinenii, supporting its potential application in sustainable urban landscaping. Full article

Cordyceps militaris (C. militaris) is a medicinal fungus renowned for its diverse therapeutic properties, largely attributed to bioactive compounds such as cordycepin, polysaccharides, adenosine, D-mannitol, carotenoids, and ergosterol. However, the production and composition of these metabolites are highly influenced by cultivation conditions, highlighting the need for systematic optimization strategies. This review synthesizes current findings on how nutritional factors—including carbon and nitrogen sources, their ratios, and trace elements—and environmental parameters such as oxygen availability, pH, temperature, and light regulate C. militaris metabolite biosynthesis. The impacts of solid-state fermentation (using grains, insects, and agro-industrial residues) and liquid state fermentation (submerged and surface cultures) are compared, with attention to their roles in mycelial growth, fruiting body formation, and secondary metabolite production. Special emphasis is placed on mixed grain–insect substrates and light regulation, which have emerged as promising methods to enhance cordycepin accumulation. Beyond summarizing advances, this review also identifies key knowledge gaps that must be addressed: (i) the incomplete understanding of metabolite regulatory networks, (ii) the absence of standardized cultivation protocols, and (iii) unresolved challenges in scale-up, including oxygen transfer, foam control, and downstream processing. We propose that future research should integrate multi-omics approaches with bioprocess engineering to overcome these limitations. Collectively, this review highlights both current progress and remaining challenges, providing a roadmap for advancing the sustainable, scalable, and application-driven production of bioactive compounds from C. militaris. Full article

The sexual generation of Schlechtendalia chinensis (Bell) is pivotal for gallnut yield yet cannot feed due to mouthpart degeneration. Could respiratory metabolic rate (RMR) modulation compensate for nutritional deficits? We quantified the RMR across key developmental stages of sexual morphs (including parental sexuparae and progeny fundatrices) using an LI-6400XT portable photosynthesis system equipped with a customized insect respiration chamber (6400-89). All morphotypes exhibited significantly lower nocturnal RMRs compared to their diurnal rates (p < 0.05), while RMRs did not differ significantly between morning (9:00–12:00) and afternoon (14:00–17:00) (p > 0.05). Significant RMR variation occurred among morphotypes: females and sexuparae displayed the lowest rates, fundatrices were intermediate, and males exhibited remarkably elevated rates (2–3 times higher than those of females or sexuparae). Both sexes showed a characteristic RMR trajectory: elevated at birth and declining during early postnatal development, followed by a gradual resurgence that culminated in peak values on postnatal day 8, coinciding with mating. This physiological zenith was immediately succeeded by marked respiratory metabolic downregulation following copulation, with RMRs decreasing substantially during the post-copulatory phase. Our findings demonstrate significant intergenerational and intersexual RMR differentiation. This research addresses critical knowledge gaps in the respiratory metabolism of S. chinensis, is the first to elucidate a nutrient adaptation strategy through respiratory metabolic regulation under non-trophic conditions, and provides actionable insights for optimizing gallnut production in controlled cultivation systems. Full article

The scope of this research was to quantify the mid-term impact of different soil tillage on carbon/nitrogen agronomical key context under optimal growing conditions of the European moderate continental climate. A large-scale on-farm experiment was established in winter wheat/soybean two-crop long-term cultivation without fertilization on fertile Luvic Chernozem. Four treatments were conducted: (T1) ‘Deep Loosening’ with tillage depth of 50 cm, (T2) ‘Plowing’ to 30 cm, (T3) ‘Strip-Till’ with tillage depth of 20 cm, and (T4) ‘No-Till’; the tillage frequency at T1 and T2 was reduced and applied to soybean only, therefore, once per 2 years during the trial period 2020/21–2024/25. Unlike the crop yield, which decreased with tillage intensity decreasing (21.38 > 19.30 > 18.88 > 18.62 t/ha in dry matter cumulatively; T2 > T3 > T1 > T4), the carbon/nitrogen key agronomical parameters either increased (root nodules count/weight: thus confirmed convergent, occasionally reverse indicators; soil compaction: penetrometric resistance) or differed in varying patterns and extent (soil chemical indicators). In fertile Chernozem soils, tillage and indicators have different importance within the nexus studied; plowing still gives the most stable yields. To improve nitrogen fixing, farmers’ practices need to balance yield vs. soil health, including eliminating soil compaction. Full article

In traditional scallion cultivation, the bare-root transplanting method—which involves direct seeding, seedling raising in the field, and lifting—is commonly adopted to minimize seedling production costs. However, during the mechanized transplanting of bare-root scallion seedlings, practical problems such as severe seedling damage and poor planting uprightness exist. In this paper, the Hertz–Mindlin with Bonding contact model was used to establish the scallion seedling model. Combined with the Plackett–Burman experiment, steepest ascent experiment, and Box–Behnken experiment, the bonding parameters of scallion seedlings were calibrated. Furthermore, the accuracy of the scallion seedling model parameters was verified through the stress–strain characteristics observed during the actual loading and compression process of the scallion seedlings. The results indicate that the scallion seedling normal/tangential contact stiffness, scallion seedling normal/tangential ultimate stress, and scallion Poisson’s ratio significantly influence the mechanical properties of scallion seedlings. Through optimization experiments, the optimal combination of the above parameters was determined to be 4.84 × 10⁹ N/m, 5.64 × 10⁷ Pa, and 0.38. In this paper, the flexible planting components of scallion seedlings were taken as the research object. Flexible protrusions were added to the planting disc to reduce the damage rate of scallion seedlings, and an EDEM-ADAMS coupling interaction model between the planting components and scallion seedlings was established. Based on this model, optimization and verification were carried out on the key components of the planting components. Orthogonal experiments were conducted with the contact area between scallion seedlings and the disc, rotational speed of the flexible disc, furrow depth, and clamping force on scallion seedlings as experimental factors, and with the uprightness and damage status of scallion seedlings as evaluation criteria. The experimental results showed that when the contact area between scallion seedlings and the disc was 255 mm², the angular velocity was 0.278 rad/s, and the furrow depth was 102.15 mm, the performance of the scallion planting mechanism was optimal. At this point, the uprightness of the scallion seedlings was 94.80% and the damage rate was 3%. Field experiments were carried out based on the above parameters. The results indicated that the average uprightness of transplanted scallion seedlings was 93.86% and the damage rate was 2.76%, with an error of less than 2% compared with the simulation prediction values. Therefore, the parameter model constructed in this paper is reliable and effective, and the designed and improved transplanting mechanism can realize the upright and low-damage planting of scallion seedlings, providing a reference for the low-damage and high-uprightness transplanting operation of scallions. Full article

In response to China’s drive to bring newly cultivated land into production, this study evaluated how combined organic fertilizer and microbial inoculants affect soil quality, bacterial community structure, and maize yield. Four treatments were evaluated: FC (chemical fertilizer only), T50 (50% organic fertilizer + 50% chemical fertilizer), T50M (T50 plus microbial inoculant), and CK (no fertilizer). T50M significantly increased yield compared to FC and CK (p < 0.05), achieving the highest yield of 6995.73 kg ha⁻¹. This was 20.09% greater than FC. Community composition analyses showed that soil in newly cultivated land was dominated by Blastocatellia, Vicinamibacteria, and Alphaproteobacteria, together accounting for over 35.7% of total bacterial abundance. Redundancy analysis at the class level explained 55.7% of variance; soil organic matter (SOM) and available potassium positively correlated with Alphaproteobacteria and Bacteroidia, while available phosphorus and nitrate nitrogen aligned with Actinobacteria and Bacilli. Path analysis indicated that SOM and total nitrogen were the strongest positive drivers of yield. Actinobacteria and Acidobacteriae also showed direct positive effects, whereas Verrucomicrobiae had a negative effect. These results demonstrate that integrated organic–microbial amendments can enhance soil fertility and alter microbial diversity toward taxa that can improve maize productivity. Full article

As a complex ecosystem, a business environment plays a structural role in shaping enterprise vitality, yet its multidimensional mechanisms remain underexplored, particularly within transitioning economies. This study employs a time-series Global Principal Component Analysis (GPCA) model to measure the vitality levels of 1475 A-share listed enterprises and the quality of business environments across 284 cities between 2008 and 2022 in China. Based on Complex Adaptive Systems (CAS) theory, we propose a three-dimensional “institution–resource–capability” theoretical framework to analyze the impact of a business environment on enterprise vitality and its underlying complex mechanisms. Our results reveal that, (1) a business environment and its constituent subsystems significantly enhance enterprise vitality, with the market environment and innovation ecosystem exhibiting the strongest effects; (2) the revitalizing impact of a business environment is more pronounced for enterprises in the tertiary industry, manufacturing, regulated sectors, and foreign-invested enterprises (FIEs), as well as those operating in Eastern China; (3) mechanistically, the positive association between a business environment and enterprise vitality is consistent with the following three core pathways: mitigating enterprise risks, restructuring resource provision, and cultivating enterprise capability. This research enriches theoretical frameworks for enterprise sustainable development within complex economic systems, while providing valuable policy implications for optimizing business environments in global transitioning economies. Full article

Global warming poses a growing threat to winter wheat production in Henan Province, a critical region for China’s food security, necessitating a quantitative assessment of climate impacts. This study aimed to quantify the dominant climatic drivers of winter wheat yield and assess its spatiotemporal evolution and future risks under climate change, thereby providing a scientific basis for targeted adaptation strategies. Thus, the APSIM model in combination with the Geodetector method was applied to quantify the spatiotemporal response of winter wheat yield to climate change in Henan Province under historical (1957–2020) and SSP245 scenarios. The study results demonstrated significant trends in climatic factors during the winter wheat growing season: precipitation decreased by an average of 3.09 mm/decade, sunshine hours declined by 36 h/decade, wind speed reduced by 0.447 m/(s·decade), and evaporation decreased by 14.7 mm/decade. In contrast, the accumulated temperature ≥ 0 °C significantly increased by 70.9 °C·d/decade. Geodetector analysis further identified accumulated temperature as the dominant climatic driver (q = 0.548), followed by precipitation (q = 0.340) and sunshine hours (q = 0.261). Yield simulations from 1960 to 2018 indicated that most regions maintained stable or slightly increasing yields (<50 kg·ha⁻¹·decade⁻¹), though some areas experienced fluctuating declines. Under future scenarios, major production regions in Henan Province (Zhengzhou, Xinxiang, Luoyang) are projected to see substantial yield increases, with growth rates of 147.2–148.9 kg·ha⁻¹·decade⁻¹. Specifically, Xinxiang is expected to achieve yields of 6200 kg·ha⁻¹. The frequency of climate-induced negative yield years decreased by approximately 35% after 2003, highlighting the role of improved agricultural technologies in enhancing climate resilience. This study clarifies how multiple climatic factors jointly affect winter wheat yield, identifying rising accumulated temperature and water stress as key future constraints. It recommends optimizing varietal selection and cultivation practices according to regional climate patterns to improve policy relevance and local applicability. Full article

Garlic, as an important economic crop, plays a crucial role in the global agricultural production system. Accurate identification of garlic cultivation areas is of great significance for agricultural resource allocation and industrial development. Traditional crop identification methods face challenges of insufficient accuracy and spatial fragmentation in complex agricultural landscapes, limiting their effectiveness in precision agriculture applications. This study, focusing on Kaifeng City, Henan Province, developed an integrated technical framework for garlic identification that combines deep learning edge detection, multi-source feature optimization, and spatial constraint optimization. First, edge detection training samples were constructed using high-resolution Jilin-1 satellite data, and the DexiNed deep learning network was employed to achieve precise extraction of agricultural field boundaries. Second, Sentinel-1 SAR backscatter features, Sentinel-2 multispectral bands, and vegetation indices were integrated to construct a multi-dimensional feature space containing 28 candidate variables, with optimal feature subsets selected through random forest importance analysis combined with recursive feature elimination techniques. Finally, field boundaries were introduced as spatial constraints to optimize pixel-level classification results through majority voting, generating field-scale crop identification products. The results demonstrate that feature optimization improved overall accuracy from 0.91 to 0.93 and the Kappa coefficient from 0.8654 to 0.8857 by selecting 13 optimal features from 28 candidates. The DexiNed network achieved an F1-score of 94.16% for field boundary extraction. Spatial optimization using field constraints effectively eliminated salt-and-pepper noise, with successful validation in Kaifeng’s garlic. Full article

Ridge and furrow planting is a prevalent drought-resistant cultivation technique in dryland regions. Notably, the effects of this technology on crop grain yield and quality in dryland maize–wheat double-cropping systems remain limited. This study utilized a long-term positioning experiment initiated in 2004, which included five treatments: a permanent ridge and furrow with a border ridge of 133 cm row space (PRFBR); a ridge and furrow created each year with a border ridge of 133 cm row space (EYRFBR); a permanent ridge with a normal ridge of 100 cm row space (PRFNR); a ridge and furrow created each year with a normal ridge of 100 cm row space (EYRFNR), and a conventional flat planting pattern according to the local farmer (CF). The crop grain yield in 2015–2021, as well as the protein and phosphorus (P) and potassium (K) content in maize and wheat grains, and the protein components in winter wheat grains in 2020–2021 were investigated. The results showed that, compared to CF, all four ridge and furrow planting patterns significantly enhanced crop yield in dry and normal years, and the effects varied depending on crop species, with increases of 45.3–97.8% for wheat and 11.0–33.8% increases annually in dry years; and 24.5–51.6% increases for maize and 12.2–37.5% increases annually in the normal years. EYRFBR treatment increased wheat grain P and K content by 24.3% and 13.7%, as well as increasing the total protein, albumin, gliadin, soluble protein, and storage protein content by 9.7%, 22.3%, 9.6%, 14.5%, and 5.6%, whereas PRFNR reduced the glutenin content and glutenin/gliadin ratio in winter wheat grains by 5.1% and 10.9%, respectively. The yield achieved with a permanent ridge and furrow (PRF) surpassed that achieved when the ridge and furrow was created anew each year (EYRF), yet the normal ridge width (NR) outperformed the border ridge width (BR). However, the P, K, protein, and protein component content in wheat grains under EYRF was superior to that under PRF. Comprehensive evaluations through principal component analysis (PCA) and TOPSIS analysis consistently demonstrated that the EYRFBR treatment delivered optimal performance in yield and quality for winter and annual, while PRFNR achieved superior yield for summer maize. Consequently, in dryland maize–wheat double-cropping systems, an EYRFBR planting pattern should be recommended for high-yield and high-quality wheat production; however, the PRFNR planting pattern is more suitable for summer maize production. Full article

In arid areas, the combined use of plastic sheeting under gravel-sand mulch on ridge-furrow planting systems is an emerging practice to minimize soil water evaporation and micro-plastic pollution. In this study, we conducted a two-year field experiment near Gobi-Tengger Desert in Ningxia, China, to evaluate the effects of a plastic film underneath a layer of pure sand (MS1), pure gravel (MS2) and mixed gravel-and-sand (MS3) mulch on the soil hydrothermal properties, water use efficiency, yield, and fruit quality of wolfberry, compared to bare soil (CK). The results showed that mulching significantly increased soil temperature and water content in the 0–20 cm surface layer, though the effects varied with soil depth and water availability between a supplemental irrigated year (2022) and a rain-fed year (2023). Mulching markedly altered soil water dynamics, enhancing the capture and retention of light-to-heavy rainfall events. Consequently, all mulches significantly increased seasonal water consumption (ET) and water use efficiency (WUE) compared to CK. The MS1 treatment consistently achieved the highest yield and WUE, and the highest accumulation of beneficial fruit compounds like polysaccharides and flavonoids. However, this treatment also resulted in elevated soil salinity. Our findings demonstrate that combined mulching, especially MS1, is a highly effective strategy for optimizing soil conditions, water productivity, and fruit quality in wolfberry cultivation, although long-term salinity management requires attention. Full article

Challenges significantly hinder the sustainable cultivation of tea chrysanthemum, leading to imbalances in soil nutrients, the accumulation of allelopathic phenolic acids, reduced enzymatic activity, and disruptions in rhizosphere microbial communities. To explore potential mitigation strategies, this study systematically evaluated the integrative effects of exogenous methyl jasmonate (MeJA, 0–400 (μmol L⁻¹)) on both soil environmental parameters and plant growth performance under continuous cropping conditions. The results revealed that treatment with 100 (μmol L⁻¹) MeJA significantly enhanced plant height, canopy width, flower number, and fresh flower weight. Concurrently, it improved soil organic matter content, the available nitrogen levels, and redox stability while increasing the activity of key enzymes, including polyphenol oxidase, urease, and catalase. Notably, this treatment markedly reduced the accumulation of allelopathic phenolic acids, such as p-hydroxybenzoic acid and vanillic acid. High-throughput sequencing further demonstrated that 100 (μmol L⁻¹) MeJA optimized the composition of soil microbial communities, increasing the abundance of beneficial taxa, such as nitrogen-fixing and phosphate-solubilizing bacteria, while suppressing pathogenic fungi. Metabolomic analysis showed that this concentration of MeJA activated stress-resistance metabolic pathways involving flavonoids and terpenoids while downregulating degradation-related processes, thereby supporting enhanced plant resilience at the metabolic level. Collectively, these findings demonstrate that an appropriate concentration of exogenous MeJA can effectively alleviate continuous cropping obstacles in Chrysanthemum morifolium, providing both theoretical insights and practical guidance for its eco-friendly and efficient cultivation. Full article

Grapes are frequently contaminated by Aspergillus section Nigri fungi and ochratoxin A (OTA), with A. niger also capable of producing substantial fumonisin B₂ (FB₂) levels. Emerging evidence suggests that aflatoxigenic fungi may eventually replace ochratoxigenic fungi in certain regions due to better adaptation to changes in climatic conditions. However, research on the toxigenic fungal community and mycotoxins in grapes from organic vineyards remains limited. Research on Spanish conventional grapes is also deficient, with most of the available literature being outdated. The present study investigates the diversity of toxigenic fungi and the presence of mycotoxins in organically cultivated grape berries in Spain, which are renowned for their significant oenological tradition. This study employed species-specific PCR protocols for fungal characterization and optimized methods for the analysis of OTA, FB₂, and aflatoxin B₁ (AFB₁) by UPLC–ESI–MS/MS. The most prevalent species present were Aspergillus flavus, A. niger, A. parasiticus, A. steynii, A. carbonarius, and A. westerdijkiae (67.1%, 43.5%, 20.0%, 14.1%, 14.1%, and 11.8% of the samples, respectively). OTA was detected only in 16 samples (19%), averaging 0.48 ng/g and peaking at 0.7 ng/g, which were lower than previously reported for conventional grapes. There was no FB₂ or AFB₁ detected. This study is pioneering in its exploration of the occurrence of toxigenic mycobiota, beyond Nigri fungi, and subsequent potential for other serious mycotoxins to contaminate Spain’s organic grapes. Full article

Graesiella emersonii, an aeroterrestrial green microalga, exhibits high adaptability to extreme environmental conditions, making it of interest for biotechnological applications. Investigating photosynthetic performance is essential to select high-yield strains and optimize the sustainable production of biomass and bio-products. In this study, two strains (053 and 054) were cultured under atmospheric (0.04%) and elevated (2%) CO₂ conditions to analyze growth, pigment content, and photosynthesis. Strain 053 showed superior photosynthetic performance and productivity under atmospheric conditions, whereas 2% CO₂ enhanced growth in both strains, with a significant increase in photosynthetic efficiency in strain 054. The observed differences highlight strain-specific adaptations to CO₂ availability and suggest the potential of each strain depending on the cultivation environment. Full article

Sustainable agricultural production systems are a global consensus. Their life-cycle economic feasibility is essential for long-term sustainable goals. This study integrates life-cycle costing with building energy simulation to assess the cost performance of conventional and innovative greenhouse tomato production systems in China’s Hexi Corridor, using dynamic thermal load modeling to overcome empirical-data limitations in traditional life-cycle costing. Under the facility-lease farming model, construction companies incur life-cycle costs of CNY 10.53·m⁻²·yr⁻¹ for the conventional concrete-walled Gobi solar greenhouse and CNY 10.45·m⁻²·yr⁻¹ for the innovative flexible insulation-walled Gobi solar greenhouses. However, farmer greenhouse contractors achieve 10.5% lower life-cycle costs for tomato cultivation in the conventional structure (CNY 2.87·kg⁻¹·yr⁻¹) than in the innovative one (CNY 3.21·kg⁻¹·yr⁻¹) due to 52.6% heating energy savings from the integrated active solar thermal systems. Furthermore, life-cycle cash flow analysis confirms construction companies incur non-viable returns, while farmers achieve substantial profits, with 52.5% higher cumulative profits obtained in the conventional greenhouse than the innovative greenhouse. This profit allocation imbalance threatens sustainability. Our pioneering stakeholder-perspective assessment provides evidence-based strategies for government, investors, and farmers to optimize resource allocation and promote sustainable Gobi agriculture. Full article