Search Results (451)

Consumers increasingly seek clean-label meat products with improved nutrition and stability. We evaluated a collagen hydrolysate–cranberry mixture (CH-CR) as a functional additive in cooked sausages. Two formulations—control and CH-CR—were assessed for fatty acid profile; lipid and protein oxidation during storage; antioxidant capacity ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and half-maximal inhibitory concentration (IC50); amino acid composition; and instrumental color. Relative to the control, CH-CR produced a more favorable lipid profile: lower saturated fatty acids (SFAs) 23.9% vs. 28.0%, higher monounsaturated fatty acids (MUFAs) 53.2% vs. 49.3%, slightly higher polyunsaturated fatty acids (PUFAs) 23.3% vs. 22.7%, a higher PUFA/SFA ratio of 0.97 vs. 0.81, and a lower omega-6/omega-3 (n-6/n-3) ratio of 13.5 vs. 27.1, driven by higher alpha-linolenic acid (ALA) 1.6% vs. 0.8%, with trans fats <0.1%. Storage studies showed attenuated oxidation in CH-CR: lower peroxide value (PV) at day 10 8.1 ± 0.4 vs. 9.8 ± 0.5 meq/kg and lower thiobarbituric acid-reactive substances (TBARS) at day 6 0.042 ± 0.004 vs. 0.055 ± 0.006 mg MDA/kg and day 10 0.156 ± 0.016 vs. 0.590 ± 0.041 mg MDA/kg); the acid value at day 10 was similar. Antioxidant capacity increased with CH-CR FRAP 30.5 mg gallic acid equivalents (GAE)/g vs. not detected; DPPH inhibition was 29.88% vs. 10.23%; IC50 56.22 vs. 149.51 µg/mL. The amino acid profile reflected collagen incorporation—higher glycine+proline+hydroxyproline 2.37 vs. 1.38 g/100 g and a modest rise in indispensable amino acids (IAAs) 5.72 vs. 5.42 g/100 g, increasing the IAA/total amino acid (TAA) ratio to 0.411 vs. 0.380. CH-CR samples were lighter and retained redness better under light, with comparable overall color stability. Overall, CH-CR is a natural strategy to improve fatty acid quality and oxidative/color stability in sausages. Full article

This study investigates whether publicly listed cannabis shares provide enough risk-adjusted returns to warrant their incorporation into diversified portfolios. An equally weighted portfolio of cannabis companies is constructed using monthly data from January 2015 to December 2024. Risk-adjusted performance is assessed using the Sharpe, Sortino, and Omega ratios and compared to the Russell 3000 Index and the FTSE All-World ex-US Index. In addition, we estimate both unconditional and conditional Fama–French five-factor model enhanced by momentum. The findings indicate that cannabis stocks persistently underperform U.S. and global benchmarks in both absolute and risk-adjusted metrics. Downside risk is elevated because cannabis portfolios exhibit much higher value at risk (VaR) and conditional value at risk (CVaR) than broad indices, especially after COVID-19. The findings show that cannabis stocks are quite volatile and fail to generate significant returns on a risk-adjusted basis. The study highlights the sector’s structural vulnerabilities and cautions investors, portfolio managers, and regulators against treating cannabis shares as dependable long-term investments. Full article

The omega-3 polyunsaturated fatty acids (n-3 PUFA) in animal-derived foods are increasingly important for human health. Sacha inchi (Plukenetia volubilis L.), rich in n-3 PUFA, is a promising feedstuff for producing n-3 PUFA-enriched animal products. However, research on dietary Sacha inchi oil (SIO) supplementation in laying hens remains limited. Therefore, this study aimed to evaluate the effects of SIO on production performance, egg quality, and yolk fatty acid (FA) composition in laying hens. A total of 192 hybrid hens [(♂ White Leghorn × ♀ Egyptian Fayoumi); initial body weight: 1910 ± 22.14 g; age: 25 weeks old] were randomly assigned one of the four diets: a basal diet (CONT), and three tested diets based on CONT with the addition of 1.5%, 3.0%, and 4.5% SIO (as-feed basis) (SI15, SI30, and SI45, respectively). Each dietary treatment included 48 hens housed in 12 battery cages for 56 days with 4 birds per cage. Increasing dietary SIO levels significantly reduced average daily feed intake (p < 0.05). Egg yolk weight linearly increased (p < 0.05) with higher SIO inclusion, while other egg quality traits were unaffected. SIO supplementation significantly increased (p = 0.001) yolk n-3 PUFA level (from 0.79% to 8.29% on day 28, and from 0.87% to 9.13% on day 56) and substantially reduced the n-6/n-3 PUFA ratio by about nine-fold on both 28 and 56 days. In conclusion, SIO is a promising n-3 PUFA-rich feed ingredient for egg production. Adding 3.0–4.5% SIO in laying hen diets can enhance egg yolk weight and n-3 PUFA level while reducing feed intake without negatively affecting egg production or quality. Full article

The reuse of by-products from plant processing as feed for animals aligns with the principles of a circular economy. Feeding dairy ruminants agro-industrial by-products often alters the chemical composition and sensory characteristics of dairy items. A dual approach—classic with neuro-sensory techniques—was utilized to evaluate the effect of prickly pear by-products on the diets of dairy ewes. Fresh Pecorino cheeses made from the milk of two groups of sheep fed with and without prickly pear by-product silage were analyzed for chemical composition and volatile organic compounds (VOCs). Furthermore, a neurosensory approach with consumers was used, combining electroencephalography (EEG) and temporal dominance of sensations techniques (TDS). Prickly pear silage in sheep diets did not alter the chemical composition of fresh cheese, but it did modify its fatty acids, with a significant increase in SFA (+2.60%) and PUFA (+0.33%), with a better n-6/n-3 ratio (−0.35%) due to higher omega-3 fatty acid content (+0.23%). The identification of VOCs revealed an increase in caproic acid (+27.27%) and n-caprylic acid (+6.47%) and a greater presence of sweet notes in the prickly pear-based cheeses, which exhibited a different aromatic complexity compared with the control cheeses. Even with a neuromarketing approach, sweetness remained the predominant sensation. Full article

The concept “we are what we eat” is gaining increasing relevance as diet-related diseases and comorbidities continue to rise, while consumers place greater emphasis on healthy lifestyles and acknowledge the pivotal role of nutrition in disease prevention. Among dietary components, omega-3 (ω-3) and omega-6 (ω-6) polyunsaturated fatty acids stand out for their broad spectrum of health benefits. This review explores their potential roles in reducing triglyceride levels, delaying the onset of neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases, preventing depression, supporting infant brain development, modulating inflammatory processes, and contributing to cancer prevention. The mechanisms of action of these fatty acids are discussed, along with their potential adverse effects—particularly the risk of interactions with anticoagulant medications, which require cautious use. While ω-3 fatty acids are widely recognized for their anti-inflammatory properties, ω-6 fatty acids exhibit both pro- and anti-inflammatory effects, highlighting the importance of achieving a balanced intake. The recommended ω-6:ω-3 ratio, ideally between 4:1 and 1:1, is emphasized as a key element in promoting informed dietary choices. This review also discusses current legislation framework on food supplements, with a focus on challenges such as the lack of stringent regulation regarding supplement content. These gaps underline the need for improved nutritional literacy and stronger regulatory oversight. Ultimately, this review emphasizes the imperative for evidence-based dietary fat recommendations, integrative public health education strategies, the revision and standardization of nutritional guidelines, and the enforcement of robust regulatory frameworks and quality-control protocols across the food supplement industry. Full article

Background/Objectives: In early-stage diabetes, diastolic dysfunction is an initial indicator of heart failure and is linked to altered glucose metabolism, including in prediabetes. Based on initial evidence that Calanus oil, derived from Calanus finmarchicus, which is rich in omega-3 polyunsaturated fatty acids and other bioactive compounds, benefits metabolic and cardiorespiratory health, this proof-of-principle study aimed to assess whether Calanus oil improves diastolic function in prediabetic women. Methods: Twenty middle-aged, obese women with prediabetes and no history of cardiac complications were enrolled and received 4 g/day of Calanus oil, providing 276 mg EPA + 256 mg DHA, for 12 weeks. Systolic and diastolic cardiac function, including the E/A ratio (E/A), was assessed by echocardiography. In addition, central blood pressure (BP) and pulse wave velocity (PWV) were analyzed by oscillometry. Metabolic health was evaluated using composite markers, including the metabolic syndrome severity score (Met-S score) and the triacylglycerol glucose–waist-to-height ratio (TyG-WHtR). Results: E/A was significantly improved (p = 0.023) following 12 weeks of Calanus oil supplementation. Furthermore, a significant improvement in metabolic health, indicated by a reduced Met-S score and a lower TyG-WHtR, was noticed (p < 0.001, respectively), reflecting decreased metabolic syndrome severity and enhanced insulin sensitivity. In addition, a significant reduction in diastolic BP, resting heart rate (p = 0.047), but not PWV or systolic BP (all p > 0.05) was observed. The improvement in E/A was associated with improved insulin sensitivity, as reflected by a decrease in the TyG-WHtR (p = 0.014). Conclusions: These exploratory findings suggest that Calanus oil supplementation in pre-diabetic women might improve central diastolic haemodynamics, accompanied by an overall improvement in metabolic health. However, the absence of a placebo control group limits definitive conclusions. Full article

The market for digital assets, and more specifically cryptocurrencies, is growing, although their adoption in small island countries remains absent. This paper explores the potential benefits of integrating cryptocurrencies into portfolios alongside stocks, with a focus on Fiji’s stock market. This is the first study on a small market like Fiji, which emphasizes the role of cryptocurrencies in portfolio management. We analyze the outcomes (returns and risks) of combining cryptocurrencies with stocks using 12 different techniques. We use monthly stock returns data of 18 companies listed on the South Pacific Stock Exchange from Aug-2019 to Jun-2025 (71 months) and nine cryptocurrencies from Sept-2019 to Jun-2025 (70 months). Our main analysis shows that only one cryptocurrency, albeit with a small exposure, consistently appears in the stock-cryptocurrency portfolios in the 12 methods. Using the return-to-risk ratio across methods as a guide, we find that the stocks-cryptocurrencies portfolio based on EQW, MinVar, MaxSharpe, MinSemVar, MaxDiv, MaxDeCorr, MaxRMD, and MaxASR offers better outcomes than the stock-only portfolios. Using high returns as a guide, we find that six out of 12 methods (EQW, MaxSharpe, MaxSort, MaxCEQ, MaxOmega, and MaxUDVol) support the stocks-cryptocurrencies portfolios. Portfolios satisfying both conditions (high return-risk ratio and high return) are supported by the EQW and MaxSharpe portfolios. The consistency of assets in both stock and stock−cryptocurrency portfolios is further confirmed by 24-month out-of-sample forecasts and Monte Carlo simulations, although the latter supports small exposures in two out of the nine cryptocurrencies. Based on the results, we conclude that a small exposure to certain cryptocurrencies can strengthen diversification and improve potential returns. Full article

Background/objectives: This study aimed to characterize the fatty acid (FA) profile of breast milk (BM) at 7 days (T7) and 1 month postpartum (T30) using gas chromatography–mass spectrometry (GC-MS) and to evaluate associations between maternal diet during pregnancy and BM FA composition. Methods: A prospective observational cohort study was conducted from March 2022 to October 2023, involving mothers grouped by gestational age at delivery (32 weeks, 32–36.6 weeks, and >37 weeks). Results: BM lipid profiles were generally similar across gestational groups, with notable differences at T7 in saturated fatty acids (SFAs), myristic acid, monounsaturated fatty acids (MUFAs), erucic acid, nervonic acid, and some FA ratios. At T30, differences persisted in SFAs, MUFAs, myristic acid, and MUFA/SFA. At T7, red meat intake was positively correlated with stearic acid; white meat intake was negatively associated with multiple FAs (including ω-3) but positively with linoleic. Cheese correlated positively with caprylic acid; milk negatively with pentadecylic acid; and dried fruit positively with MUFA. At T30, fish consumption was prevalently positively related to DHA, EPA, and Omega-3, while red meat was positively associated with arachidic acid and margaric acid and negatively with di-homo-gamma linolenic acid. White meat showed a predominantly negative correlation with DHA, EPA and MUFA. Milk intake showed both positive (i.e., caproic acid) and multiple negative FA associations. Cheese was positively associated with caprylic acid, while dried fruit intake was positively linked to oleic acid and MUFA. Conclusions: Despite stable total lipid content, gestational age influenced specific FA profiles. These shifts may reflect adaptive responses to neonatal metabolic and neurodevelopmental needs. Understanding such mechanisms could guide tailored nutritional strategies, especially for preterm infants. Full article

Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder characterized by reproductive and metabolic abnormality disorders. Dietary factors influence the body composition and hydration status, which may exacerbate PCOS symptoms. The aim of this study was to assess the associations between the habitual nutrient intake and bioelectrical impedance analysis parameters in Polish women with PCOS and healthy controls, in order to identify potential nutritional targets for a non-pharmacological intervention. Methods: This study involved 50 women aged 18–45 years (25 with PCOS and 25 healthy). Participants kept 7-day food diaries and their body composition was assessed using the SECA mBCA 515 analyzer. The nutrient intake was compared with EFSA recommendations. Results: Women with PCOS had a higher body weight, waist circumference and body mass index, visceral adipose tissue, and fat mass index, despite no difference in their total energy intake. They consumed more omega-3 fatty acids (EPA + DHA) than the control group. Vitamin D deficiency and irregular supplementation were common in both groups. Body composition parameters such as the phase angle and ECW/TBW ratio correlated with the diet quality—especially with protein; fiber; and vitamin B2, B12, and folate levels. Conclusions: The obtained results showed significant differences in body compositions and the presence of a relationship between the nutrient intake and bioimpedance parameters in women with PCOS. These results emphasize the importance of a comprehensive nutritional and body composition assessment in planning dietary interventions in this group of patients. Full article

This study evaluated the effects of dietary supplementation with omega-3 polyunsaturated fatty acids from extruded linseed, alone and combined with Padina pavonica algae extract, on growth performance and metabolic status in fattening rabbits. Sixty New Zealand White rabbits were assigned to three groups, as follows: control (CNT), L (5% linseed), and LPP (3.5% linseed + 0.2% algae extract) from weaning (37 days) to slaughter (85 days). Productive performance was assessed through body weight (BW), average daily gain (ADG), feed conversion ratio (FCR), and feed intake (FI). Blood was sampled at weaning, 60 days, and slaughter and analyzed for insulin, leptin, cortisol, thyroid hormones (T3, T4), glucose, and non-esterified fatty acids (NEFAs). The L group showed significantly higher ADG (41.0 ± 1 g/d) and improved FCR (4.1 ± 0.2) compared to LPP (ADG: 37 ± 1 g/d, FCR: 4.6 ± 0.2; p = 0.001). No differences were observed in final BW or FI among groups (p < 0.001). Insulin peaked at 60 days across all groups (p < 0.001), with the LPP group showing the lowest levels (9.8 ± 0.9 µUI/mL; p = 0.043). T3 and T4 increased significantly with age (p < 0.001), and the T3/T4 ratio varied by diet and time (p = 0.005). Cortisol rose only at slaughter (p < 0.001) and negatively correlated with insulin and thyroid hormones. The results suggest that omega-3-rich nutraceuticals can enhance growth performance without disrupting metabolic balance and may modulate specific hormonal responses due to their bioactive compounds. Full article

Introduction: Gastrointestinal (GI) cancers are associated with high morbidity and mortality. Surgical resection, the primary treatment, often induces immunosuppression and increases the risk of postoperative complications. Perioperative immunonutrition (IMN), comprising formulations enriched with omega-3 fatty acids, arginine, nucleotides, and antioxidants, has emerged as a potential strategy to improve surgical outcomes by reducing complications, enhancing immune function, and promoting recovery. Methods: A systematic search of PubMed, Scopus, and the Cochrane Library was conducted on 28 October 2024 in accordance with PRISMA guidelines. Systematic reviews and meta-analyses evaluating perioperative IMN versus standard care in adult patients undergoing GI cancer surgery were included in the search. The outcomes assessed included infectious and non-infectious complications, wound healing, hospital stay, and nutritional status. The study quality was evaluated using AMSTAR 2, and the meta-analysis was conducted using random-effects models to calculate the pooled effect sizes (risk ratios [RRs], odds ratios [ORs], mean differences [MDs]) with 95% confidence intervals (CIs). Results: Sixteen systematic reviews and meta-analyses, including a total of 41,072 patients, were included. IMN significantly reduced infectious complications (RR: 0.62, 95% CI: 0.55–0.70; I² = 63.0%), including urinary tract infections (RR: 0.74, 95% CI: 0.61–0.89; I² = 0.0%) and wound infections (OR: 0.64, 95% CI: 0.55–0.73; I² = 34.4%). Anastomotic leak rates were notably lower (RR: 0.68, 95% CI: 0.62–0.75; I² = 8.2%). While no significant reduction in pneumonia risk was observed, non-infectious complications decreased significantly (RR: 0.83, 95% CI: 0.75–0.92; I² = 30.6%). IMN also reduced the length of hospital stay by an average of 1.92 days (MD: −1.92, 95% CI: −2.36 to −1.48; I² = 73.5%). Conclusions: IMN provides significant benefits in GI cancer surgery, reducing complications and improving recovery. However, variability in protocols and populations highlight the need for standardization and further high-quality trials to optimize its application and to validate its efficacy in enhancing surgical care. Full article

The gut microbiota, dominated by bacteria from the Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria phyla, plays an essential role in fermenting indigestible carbohydrates, regulating metabolism, synthesizing vitamins, and maintaining immune functions and intestinal barrier integrity. Dysbiosis is associated with obesity development. Shifts in the ratio of Firmicutes to Bacteroidetes, particularly an increase in Firmicutes, may promote enhanced energy storage, appetite dysregulation, and increased inflammatory processes linked to insulin resistance and other metabolic disorders. The purpose of this literature review is to summarize the current state of knowledge on the relationship between the development and treatment of obesity and overweight and the gut microbiota. Current evidence suggests that probiotics, prebiotics, synbiotics, and fecal microbiota transplantation (FMT) can influence gut microbiota composition and metabolic parameters, including body weight and BMI. The most promising effects are observed with probiotic supplementation, particularly when combined with prebiotics, although efficacy depends on strain type, dose, and duration. Despite encouraging preclinical findings, FMT has shown limited and inconsistent results in human studies. Diet and physical activity are key modulators of the gut microbiota. Fiber, plant proteins, and omega-3 fatty acids support beneficial bacteria, while diets low in fiber and high in saturated fats promote dysbiosis. Aerobic exercise increases microbial diversity and supports growth of favorable bacterial strains. While microbiota changes do not always lead to immediate weight loss, modulating gut microbiota represents an important aspect of obesity prevention and treatment strategies. Further research is necessary to better understand the mechanisms and therapeutic potential of these interventions. Full article

The present study aimed to evaluate the impact of two dietary regimens, characterized by different forage-to-concentrate ratios, on feed intake, milk yield and composition, as well as economic and environmental sustainability in lactating buffalo cows. Group H was fed a high-forage diet, while Group L was fed a diet with a lower forage-to-concentrate ratio. Despite a lower energy value (Unit for Feed Lactation: UFL 0.89 vs. 0.91), the diet of group H met the animals’ nutritional requirements, with similar dry matter intake (DMI) and body condition scores observed in both groups. While there was no significant difference in milk yield, protein, and lactose, group H exhibited significantly higher milk fat content and an enhanced fatty acid profile, characterized by increased oleic acid, conjugated linoleic acids (CLAs), and omega-3 polyunsaturated fatty acids. These fatty acids have been linked to potential health benefits in humans, including anti-inflammatory, cardioprotective, and anticarcinogenic effects. From an economic perspective, the H group’s diet was found to be more cost-effective, resulting in EUR 0.46 savings per head per day and a higher yield of mozzarella cheese. From an environmental perspective, the high-forage diet reduced reliance on imported concentrates and irrigation-dependent crops, aligning with sustainable development goals. In conclusion, increasing the forage content in buffalo diets has been demonstrated to support animal performance, improve milk quality, reduce environmental impact, and enhance economic returns for producers. Full article

Background/Objectives: Growing interest in natural, health-promoting ingredients for functional foods, nutraceuticals, and cosmetics has increased the demand for bioactive compounds from kiwi (Actinidia deliciosa). This study aimed to assess the antioxidant, anti-inflammatory, and antithrombotic properties of amphiphilic bioactives extracted from kiwi fruit and its by-products, including peel, seeds, and pulp. Methods: Bioactive compounds were extracted and analyzed using liquid chromatography–mass spectrometry (LC–MS) and attenuated total reflectance–Fourier transform infrared (ATR–FTIR) spectroscopy. Antioxidant activity was evaluated using DPPH and ABTS radical scavenging assays. Anti-inflammatory and antithrombotic effects were assessed through inhibition of platelet aggregation induced by platelet-activating factor (PAF) and adenosine diphosphate (ADP) in human platelets. Results: All extracts showed significant antioxidant activity. FTIR and LC–MS analyses confirmed the presence of phenolics, flavonoids, carotenoids, and polar lipids. Kiwi peel extract exhibited the strongest inhibition of PAF- and ADP-induced platelet aggregation, attributed to its higher content of phenolics and unsaturated polar lipids. LC–MS data indicated a favorable fatty acid profile with high omega-9 levels and a low omega-6/omega-3 ratio. Polar lipid structural analysis revealed a predominance of phospholipids with unsaturated fatty acids at the sn-2 position. Conclusions: Kiwi by-products are valuable sources of health-promoting bioactives with antioxidant and anti-inflammatory potential. These findings support their incorporation into nutraceutical, nutricosmetic, and cosmeceutical products and lay the groundwork for further studies on safety, efficacy, and practical application. Full article

Background: An unbalanced dietary intake of omega-6 (n-6) and omega-3 (n-3) polyunsaturated fatty acids (PUFAs) has been associated with chronic inflammation and oxidative stress, both of which contribute to the pathophysiology of obesity. Objective: We aimed to evaluate the effects of a diet with an n-6:n-3 PUFA ratio of 5:1 on body composition, biochemical parameters, and the gene expression of cytokines and antioxidant enzymes in a murine model of diet-induced obesity. Methods: A diet-induced obesity model was established in C57BL6/J mice over 17 weeks. Mice were then fed different diets for 8 weeks: a control diet (chow), a high-fat diet with a 30:1 n-6:n-3 ratio (HFD-30:1), and a high-fat diet enriched with n-3 fatty acids, with a 5:1 n-6:n-3 ratio (HFD-5:1). Body weight and food intake were monitored throughout this study. Biochemical parameters were measured, and the expression of antioxidant enzymes and cytokine genes was analyzed by qPCR. Data were analyzed using GraphPad Prism software. Results: The HFD-5:1 group exhibited a significant reduction in body weight (p = 0.0182), liver tissue weight (p = 0.01), serum glucose levels (p = 0.010), area under the curve (AUC) (p = 0.0161), cholesterol (p < 0.0001), and triglycerides (p = 0.0069) compared to the HFD-30:1 group. The body weight in the HFD-5:1 group decreased to levels comparable to the control group. Additionally, the expression of the inflammatory cytokine genes Ccl2 (p = 0.0389) and Tgfb1 (p = 0.0226) was significantly reduced. Conclusions: These findings suggest that adjusting the dietary n-6:n-3 ratio to 5:1 modulates inflammation-related gene expression and improves metabolic markers in obese mice, supporting its potential relevance for future translational research. Full article