Search Results (990)

Durum wheat productivity in Mediterranean regions faces growing challenges from drought and heat stress. Understanding the genetic architecture of diverse germplasm is therefore essential to support pre-breeding efforts and enhance stress adaptation. In this context, 125 durum wheat genotypes were evaluated for agro-morphological traits across two contrasting Algerian locations over two growing seasons. A subset of 94 genotypes, selected on the basis of phenotypic characterization, was genotyped using the Illumina 7K SNP array. Population structure analysis revealed two to four subgroups, with linkage disequilibrium decaying at 4.09 Mb. Genome-wide association analysis identified 27 distinct significant SNPs associated with eight traits, with most associations detected for spike length, thousand-kernel weight, and plant height. The marker TGWA25K-TG0010 on chromosome 4A showed pleiotropic effects on plant height and peduncle length and co-localized with the Dwarf8 and gibberellic-acid-insensitive genes. Additionally, wsnp_Ex_c2033_3814035 on chromosome 2A was associated with heading earliness and the number of fertile spikelets per spike, and wsnp_Ku_c51039_56457361 on chromosome 5A with plant height and peduncle length in a single site and season. Several other environment-specific associations were also identified. These results support future studies in which the identified markers may be deployed in breeding strategies aimed at improving yield stability and stress adaptability in durum wheat under Algerian conditions. Full article

Triticale (×Triticosecale Wittmack), a synthetic hybrid of wheat (Triticum spp.) and rye (Secale cereale), is a valuable dual-purpose crop for its high yield and stress tolerance. Introducing beneficial alien chromatin is crucial for expanding genetic diversity and improving cultivars. This study aimed to introduce Thinopyrum intermedium St genome chromatin into hexaploid triticale via trigeneric hybridization to develop novel germplasm. Six stable lines were selected from crosses between an octoploid wheat-Th. intermedium partial amphiploid line Maicao 8 and a hexaploid triticale cultivar Hashi 209. Agronomic traits were evaluated over two cropping seasons, revealing that the translocation lines exhibited superior agronomic performance compared to the parental triticales. These lines showed longer spikes, higher tiller numbers, and increased grain protein content, without compromising thousand-kernel weight. Cytogenetic analysis using sequential multicolor genomic in situ hybridization (smGISH), fluorescence in situ hybridization (FISH), and oligonucleotide probes, alongside validation with species-specific molecular markers, identified all six lines as St-R terminal translocation lines containing 14 rye chromosomes. Three lines carried a small terminal St segment on chromosome 1R, while the other three carried St segments on both 1RL and 4RS chromosomes. This work demonstrates that trigeneric hybridization is an effective strategy for inducing intergeneric recombination between Thinopyrum intermedium and rye chromosomes, leading to stable, small-segment terminal translocations. The developed St-R translocation lines represent a novel and valuable germplasm resource for enriching genetic diversity and breeding improved triticale cultivars with enhanced yield and quality traits. Full article

Straw returning has been evidenced to be an effective strategy for straw utilization. Nevertheless, numerous adverse effects on straw direct returning have been widely reported. It is safer to fully degrade the straw and then return it to the soil. This strategy has been shown to significantly improve soil nutrients. However, the effect on rice growth is unclear. In this study, a pot experiment was conducted by using different types of mushroom residues with chemical fertilizer for field return, compared to the conventional method of applying chemical fertilizer alone, and straw return with or without fertilizer. It was found that the return of mushroom residues to the field could increases the soil organic matter content by 16.9–23.5%, the alkaline nitrogen by 39.1–47.4%, and the available potassium by 6.8–10.8%. Furthermore, mushroom residues were found to reduce the population of fungi and bacteria in the soil to a certain extent and increase the number of actinomycetes. Moreover, it was determined that mushroom residues elevated the nutrient content in plants, accelerated the tillering rate, and increased the number of tillers. This ultimately led to an increase in rice yield components, such as thousand-grain weight and the effective number of spikes. Mushroom residues can mimic the benefits of straw return while minimizing potential harm to rice yields. This study provides an effective strategy for the resource utilization of straw. Full article

People living with HIV-1 (PLWH) are part of the so-called “fragile” populations to which COVID-19 vaccines were/are strongly recommended. The fact that most widely used COVID-19 vaccines rely on the production of a biologically active SARS-CoV-2 Spike protein expressed by synthetic mRNA poses the relevant question of whether and how this vaccination influences the fate of the HIV-1 reservoir. This report presents a detailed analysis of the literature data on the effects of SARS-CoV-2 Spike and COVID-19 vaccines on HIV-1 latently infected cells. Despite being limited in number, the experimental evidences consistently indicate that vaccine mRNA and/or SARS-CoV-2 Spike can effectively reactivate latent HIV-1. This conclusion has been drawn after “in vitro”, “ex vivo”, and “in vivo” assays, and with virus-associated Spike, soluble Spike, or its intracellular expression, as well as with COVID-19 mRNA vaccines. On the other hand, real-world observations on vaccinated PLWH under antiretroviral therapy (ART) provided evidence of HIV-1 reactivation almost exclusively in PLWH with unsuppressed viremia, as measured in terms of size of the HIV-1 reservoir. Although several issues still need to be clarified through urgent additional investigations, these data suggest the possibility that the Spike protein and/or the vaccine mRNA molecules affect the HIV-1 latency in PLWH. Full article

This work investigates the differences between the monthly and 13-month smoothed sunspot numbers since 1749, using data from sunspot number (version 2). The distribution of the differences is centered near zero, with 74% of all values lying between −20 and +20, and only 1% exceeding ±70. Positive and negative differences are nearly balanced in total number, although the distribution of the monthly differences is moderately asymmetric (skewness = −0.55) and high kurtosis (>3), confirming leptokurtic behavior with sharper peak around zero and heavier tails than a Gaussian distribution. Spikes, defined in each tail using the 95th and 5th percentile, occurred in nearly all solar cycles studied, predominantly around solar cycle maxima and in cycles with higher solar activity. Moreover, the five most extreme cases with a difference of more than 100 occur in five solar cycles, ranging from below to above average. Additionally, we analyze the recent behavior of Solar Cycle 25. The significant increase in the monthly sunspot number in August 2024 (it reached 216, the highest since 2001) raised questions about the potential future intensity of Solar Cycle 25. As the sunspot number series evolved, this difference between the maximum monthly and 13-month smoothed series decreased to 55.1 (with data through October 2025), placing Solar Cycle 25 within the historical relationship between maximum monthly and 13-month smoothed sunspot number, and the largest monthly deviations. Our results show that spikes are a recurrent feature of solar activity and can provide useful diagnostics for shorter-term solar cycle variability. Full article

SARS-CoV-2, the virus responsible for coronavirus disease COVID-19, is a highly transmissible pathogen that has caused substantial global morbidity and mortality. The ongoing COVID-19 pandemic caused by this virus has had a significant impact on public health and the global economy. One approach to combating COVID-19 is the development of broadly neutralizing antibodies for prevention and treatment. In this work, we performed an in silico ligand-based screening of the PDB database to search for unique anti-SARS-CoV-2 motifs. The collected data were organized and presented in a classified SARS-CoV-2 Ligands Database, categorized based on the number of ligands and structural components of the spike glycoprotein. The database contains 1797 entries related to the structures of the spike glycoprotein (UniProt ID: P0DTC2), including both full-length molecules and their fragments (individual domains and their combinations) with various ligands, such as angiotensin-converting enzyme II and antibodies. The database’s capabilities allow users to explore various datasets according to the research objectives. To search for motifs in the receptor-binding domain (RBD) most frequently involved in antibody binding sites, antibodies were classified into four classes according to their location on the RBD; for each class, special binding motifs are revealed. In the RBD binding sites, specific tyrosine-containing motifs were found. Data obtained may help speed up the creation of new antibody-based therapies, and guide the rational design of next-generation vaccines. Full article

Soil salinity is a major constraint to wheat production worldwide. Efficient screening of salt-tolerant cultivars is essential for breeding programs, yet a rapid and reliable evaluation system based on full-life-cycle salt stress treatment is lacking. To address this, we conducted a hydroponic experiment encompassing the entire growth cycle of 37 wheat cultivars under control and salt stress (85.5 mM NaCl). Using principal component and stepwise regression analyses on 15 agronomic and yield-related traits, we identified five key indicators—total dry weight, root dry weight, plant height, thousand-grain weight, and number of grains per spike—that effectively represent overall salt tolerance. Based on a comprehensive evaluation value (D-value), the cultivars were classified into five distinct categories: highly salt-tolerant, salt-tolerant, moderately salt-tolerant, weakly salt-tolerant, and salt-sensitive. Notably, the highly salt-tolerant cultivar ‘Yangfumai 8′ and the salt-sensitive cultivar ‘Yangmai 22’ were selected as representative extremes. A subsequent pot experiment confirmed significant physiological differences between them in antioxidant enzyme activities (SOD, POD, CAT) and proline accumulation under salt stress. This study establishes a practical and efficient screening framework, providing breeders with a simplified index set for high-throughput evaluation and offering ideal contrasting materials for in-depth physiological research on salt tolerance mechanisms in wheat. Full article

Background and Objectives: SARS-CoV-2 produces potentially pathogenic molecules, such as single-stranded RNA and spike proteins, which can potentially activate microglial cells. In this study, we aimed to investigate whether SARS-CoV-2 spike protein S1 and mRNA vaccines can cause neurotoxicity directly or through microglial involvement. Materials and Methods: Primary cerebellar granule cell cultures isolated from Wistar rats and organotypic hippocampal slice cultures from transgenic C57BL/6J mice were used in the experiments. Imaging and quantitative analysis of cell viability, proliferation, and phagocytic activity were performed using light and fluorescence microscopy. Results: The exogenous SARS-CoV-2 S1 protein at 50 µg/mL concentration induced neuronal cell death in neuronal–glial co-cultures and stimulated microglial proliferation during the first 3 days of exposure without an effect on inflammatory cytokine secretion. Single application of Tozinameran/Riltozinameran and Original/Omicron BA. 4–5 vaccines did not affect neuronal viability and total neuronal number in cell co-cultures after 7 days of exposure. In contrast, three repeated treatments with mRNA vaccines at 6 ng/mL caused microglial proliferation without affecting microglial phagocytosis and TNF-α release. In organotypic brain slice cultures, only Tozinameran/Riltozinameran stimulated microglial cell proliferation in female brain slices, while male brain slices remained unaffected by both vaccines, indicating sex-dependent effects. Conclusions: The findings suggest that mRNA vaccines do not exert neurotoxic effects in primary neuronal–glial co-cultures, but induce microglial proliferation, particularly in female brains in the absence of inflammatory cytokine release. SARS-CoV-2 S1 protein at high concentrations directly induces neuronal death. Full article

Background/Objectives: Despite clonal expansion during a primary immune response, or after subsequent antigen encounters, the frequency of memory B cells (B_mem) specific for an antigen remains low, making their detection difficult. However, unlike serum antibodies, which have a short half-life in vivo and thus require continuous replenishment to maintain stable titers, circulating B_mem are long-lived; they preserve immunological preparedness through their ability to rapidly engage in recall responses and differentiate into antibody-secreting cells (ASCs) upon antigen encounter. To this end, development of assays suited for the reliable detection of rare antigen-specific B_mem is critical and can provide insights into an individual’s antigen exposure history and immune status beyond that offered by traditional serum antibody measurements alone. Methods: ImmunoSpot^® has emerged as a suitable technique for the detection of individual antigen-specific B cells through visualizing their antibody-derived secretory footprints. Here, we report the theoretical and practical foundations for detecting rare antigen-specific B_mem in human peripheral blood mononuclear cells (PBMC). Leveraging the unique availability of verifiably naïve vs. antigen-experienced human samples, we used SARS-CoV-2 Spike (S-) and Nucleocapsid (NCAP) antigens to interrogate the presence of B_mem with these respective specificities. Results: While 100% diagnostic accuracy was achieved for both antigens, detection of NCAP-specific B_mem required reducing the lower detection limit of the standard assay. Specifically, this was achieved by testing a total of 2 million PBMC across multiple replicate assay wells and assessing the cumulative number of secretory footprints detected. Conclusion: The protocols described here should facilitate the reliable detection of ASCs present at varying precursor frequencies and serve as guidance for routine immune monitoring of rare B_mem with specificity for any antigen. Full article

Irrigation practices and nitrogen (N) addition play pivotal roles in wheat production, and their rational coordination can significantly enhance N, phosphorus (P), and potassium (K) use efficiency and yield of wheat. However, the comprehensive effects of irrigation practices and N addition rates on N, P, and K accumulation and utilization and yield of wheat in dryland remain unclear. A field experiment with two irrigation practices (W0, zero-irrigation and W1, one-off irrigation), and four N addition rates (0, 120, 180, and 240 kg N ha⁻¹, represented by N0, N120, N180, and N240, respectively) was conducted in 2021–2022 and 2023–2024. Compared to W0N0, W1N180 significantly increased wheat grain yield, spike number, and grains per spike by 46.4%, 35.9%, and 18.9%, respectively. Wheat yield and N, P, and K accumulation reached the maximum value at N180 or N240. One-off irrigation significantly improved the uptake efficiency and fertilizer partial factor productivity for N, P, and K, whereas increased N addition enhanced these parameters specifically for P and K. However, N180 treatment increased N uptake efficiency, N fertilizer partial factor productivity, P internal efficiency, and K internal efficiency by 22.2%, 31.1%, 9.4%, and 5.9%, respectively, compared to N240 under one-off irrigation. In addition, W1N180 significantly increased above-ground N, P, and K accumulation by 45.8%, 52.8%, and 51.8%, as well as pre-anthesis N and P translocation by 48.5% and 47.0%, respectively, compared to W0N120. Consequently, the W1N180 strategy not only improved wheat yield but also optimized N, P, and K accumulation, pre-anthesis N and P translocation, and nutrient use efficiency. Therefore, one-off irrigation combined with N180 can be recommended for enhancing wheat yield and nutrient use efficiency in dryland. Full article

Delayed sowing has become a key constraint on winter wheat production in the Guanzhong Plain, Shaanxi Province, China, due to the widespread adoption of late-maturing maize and the delayed harvest of preceding crops. A two-year field experiment was conducted on the Guanzhong Plain to elucidate the physiological mechanisms behind yield reduction under delayed sowing and to explore potential mitigation strategies. The study examined the effects of sowing time (normal, 10-day delay, and 20-day delay) and plastic film mulching on yield components, crop development, and water and nitrogen uptake and use in winter wheat. Compared to normal sowing, delayed sowing significantly reduced grain yield (7.64–17.19%), spike number (11.65–21.3%), 1000-grain weight (5.2–9.05%), growth duration (7–16 d), dry matter accumulation (21.79–58.07%), and partial factor productivity of nitrogen fertilizer (7.64–17.2%). Late sowing slowed overall growth and development, shortened the growth cycle, and suppressed root system expansion and plant height, particularly under the 20-day delay. However, plastic film mulching under delayed sowing improved seedling emergence, root growth, tiller number (8.42–51.23%), water use efficiency (10.15–18.15%), and nitrogen productivity, thereby mitigating the adverse effects of delayed sowing on resource capture. Mulching enabled wheat sown with a 10-day delay to achieve yields comparable to normal-sown crops and alleviated 9.1–10.3% of the yield loss under a 20-day delay, although it did not fully restore yields to the non-delayed level. These findings provide practical insights for managing winter wheat under delayed sowing conditions. Full article

This study aimed to develop a methodology to evaluate, through RGB image processing, the wheat cultivar TRIO Calibre under three irrigation levels (100, 50, and 25%), with or without the application of Bacillus aryabhattai, in Brazilian Cerrado soil. The experimental scheme was a $3\times 2$ factorial design with five replicates. Images were collected, numbered, and organized into files, which were transformed to grayscale. During processing, the grayscale level co-occurrence matrix (GLCM) technique was applied and implemented in four main directions (0°, 45°, 90°, and 135°), and 13 statistical descriptors were extracted. At physiological maturity, the plants were harvested, and the following yield components were evaluated: plant height (PH), number of spikes per plant (NS), number of grains per spikes (NGS), average grain weight (AGW), and total prodution of grains (TPG). Irrigation influenced all the variables, with higher TPG and NS at 100% and 50% water and higher AGW at 25% water. The results indicated that the “contrast” descriptor in the 90° and 135° GLCM directions was the most efficient in differentiating treatments, which presented better performance in the 90° direction and was significantly correlated with the NS ($r=-0.48$, $p<0.05$) and TPG ($r=-0.46$, $p<0.05$). The analyses demonstrated that the methodology has the potential to be adapted for the analysis of under controlled conditions, contributing to more sustainable agricultural practices. Full article

During the COVID-19 pandemic, the prevalence of death in men was higher than in women. Using transgenic mice expressing the human angiotensin-converting enzyme 2 (hACE2), we demonstrated that SARS-CoV-2 infects Leydig cells and uses its steroidogenic machinery for replication. This study investigates the impact of SARS-CoV-2 in the seminiferous epithelium of K18-hACE2 mice, focusing on the immune response, junctional proteins, and spermatogenesis. The seminiferous tubules (STs) and epithelial (EA) areas were measured. The number of Sertoli cells (SCs), spermatocytes, and damaged ST was quantified. Ultrastructural analysis was performed under transmission electron microscopy. Angiotensin II levels and immunolocalization of hACE2, spike, and nucleocapsid were evaluated. TUNEL and immunoreactions for Ki-67, TNF-α, INF-γ, iNOS, NF-κB, and Conexin-43 were performed and correlated with Jam-α, Stat1, Stat3, and iNOS expressions. hACE2, spike, and nucleocapsid immunolabeling were detected in the epithelium along with high angiotensin II levels in the infected mice. The infection caused a significant reduction in ST, EA, spermatocytes, SCs, Ki-67+ cells, Cx43 immunoexpression, and Jam-a expression. In the epithelium, TNF-α, IFN-γ, iNOS, and nuclear NF-κB immunolabeling increased along with Stat1 upregulation. These findings, combined with the increased epithelial hACE2 and high angiotensin II levels, confirm epithelial responsiveness to the infection and explain the spermatogenic failure and impaired junctional proteins. The presence of viral particles, increased TNF-α immunolabeling, and apoptotic features in Sertoli cells suggests that these sustentacular cells are targets for viral infection in the epithelium, and, due to their extensive projections and ability to phagocytize dying infected germ cells, they may disseminate the viruses throughout the epithelium. Full article

Increasing planting density is an effective measure to mitigate the negative impacts of late-sowing on yield formation in winter wheat. However, the physiological mechanisms underlying source-sink coordination and high-yield performance through density regulation in hybrid wheat with high yield potential remain unclear. A two-year field experiment was conducted using the hybrid variety Jingmai 17 and conventional variety Jimai 22 as experimental materials, with three planting densities: 150 plants·m⁻² (M1), 300 plants·m⁻² (M2), and 450 plants·m⁻² (M3). The effects of planting density on the source-sink relationship and yield were systematically investigated. The results showed that both Jingmai 17 (2.4–9.7%) and Jimai 22 (1.4–10.6%) exhibited the most significant yield increases under the M2 treatment. This density maintained photosynthetic capacity during the mid-to-late grain-filling stage, delayed leaf senescence, promoted assimilate translocation to the grains, and simultaneously improved grain number per spike and thousand-grain weight by optimizing source-sink coordination efficiency. Compared with Jimai 22, the hybrid wheat Jingmai 17 demonstrated a significant yield advantage (8.2–10.1%), which was attributed to its stronger and more persistent source function, larger and more stable sink capacity, and higher source-sink coordination efficiency. In conclusion, under late-sowing conditions, the hybrid variety Jingmai 17 at a density of 300 plants·m⁻² achieved the most effective optimization of the source-sink relationship, fully exploited its yield potential, and achieved a balance between high and stable yield. This study provides a theoretical and practical cultivation reference for the selection of hybrid wheat varieties in this region. Full article

Circuit implementation is a widely accepted method for validating theoretical insights observed in chaotic systems. It also serves as a basis for numerous chaos-based engineering applications, including data encryption, random number generation, secure communication, neuromorphic computing, and so forth. To get feasible, compact, and cost-effective circuit implementations of chaotic systems, the underlying mathematical model may be simplified while preserving all rich nonlinear behaviors. In this framework, this manuscript presents a simplified Hopfield Neural Network (HNN) capable of generating a broad spectrum of complex behaviors using a minimal number of electronic elements. Based on Shil’nikov’s theorem for heteroclinic orbits, the number of non-zero synaptic connections in the matrix weights is reduced, while simultaneously using only one nonlinear activation function. As a result of these simplifications, we obtain the most compact electronic implementation of a tri-neuron HNN with the lowest component count but retaining complex dynamics. Comprehensive theoretical and numerical analyses by equilibrium points, density-colored continuation diagrams, basin of attraction, and Lyapunov exponents, confirm the presence of periodic oscillations, spiking, bursting, and chaos. Such chaotic dynamics range from single-scroll chaotic attractors to double-scroll chaotic attractors, as well as coexisting attractors to transient chaos. A brief security application of an S-Box utilizing the presented HNN is also given. Finally, a physical implementation of the HNN is given to confirm the proposed approach. Experimental observations are in good agreement with numerical results, demonstrating the usefulness of the proposed approach. Full article