Search Results (1,090)

In the present study, we evaluated the potential use of a humic substance (HS)-based biostimulant in mitigating drought stress in lettuce (Lactuca sativa L.) by comparing both root and foliar modes of application. To achieve this, lettuce plants were grown in a growth chamber on a solid substrate composed of vermiculite and perlite (3:1). Plants were exposed to drought conditions (50% of Field Capacity, FC) and 50% FC + HS applied as radicular (‘R’) and foliar (‘F’) at concentrations: R-HS 0.40 and 0.60 mL/L, respectively, and 7.50 and 10.00 mL/L, respectively, along with a control (100% FC). HSs were applied three times at 10-day intervals. Plant growth, nutrient concentration, lipid peroxidation, reactive oxygen species (ROS), and enzymatic and non-enzymatic antioxidants were estimated. Various photosynthetic and chlorophyll fluorescence parameters were also analyzed. The results showed that HS applications alleviated drought stress, increased plant growth, and reduced lipid peroxidation and ROS accumulation. HSs also improved the net photosynthetic rate, carboxylation efficiency, electron transport flux, and water use efficiency. Although foliar HSs showed a greater tendency to enhance shoot growth and photosynthetic capacity, the differences between the application methods were not significant. Hence, in this preliminary work, the HS-based product evaluated in this study demonstrated potential for alleviating drought stress in lettuce plants at the applied doses, regardless of the mode of application. This study highlights HS-based biostimulants as an effective and sustainable tool to improve crop resilience and support sustainable agriculture under climate change. However, further studies under controlled growth chamber conditions are needed to confirm these results before field trials. Full article

The cosmetics industry has been seeking to develop products with renewable natural ingredients to reduce the use of or even replace synthetic substances. Biosurfactants can help meet this demand. These natural compounds are renewable, biodegradable, and non-toxic or have low toxicity, offering minimal risk to humans and the environment, which has attracted the interest of an emerging consumer market and, consequently, the cosmetics industry. The aim of the present study was to produce a biosurfactant from the yeast Starmerella bombicola ATCC 22214 cultivated in a mineral medium containing 10% soybean oil and 5% glucose. The biosurfactant reduced the surface tension of water from 72.0 ± 0.1 mN/m to 33.0 ± 0.3 mN/m after eight days of fermentation. The yield was 53.35 ± 0.39 g/L and the critical micelle concentration was 1000 mg/L. The biosurfactant proved to be a good emulsifier of oils used in cosmetic formulations, with emulsification indices ranging from 45.90 ± 1.69% to 68.50 ± 1.10%. The hydrophilic–lipophilic balance index demonstrated the wetting capacity of the biosurfactant and its tendency to form oil-in-water (O/W) emulsions, with 50.0 ± 0.20% foaming capacity. The biosurfactant did not exhibit cytotoxicity in the MTT assay or irritant potential. Additionally, an antioxidant activity of 58.25 ± 0.32% was observed at a concentration of 40 mg/mL. The compound also exhibited antimicrobial activity against various pathogenic microorganisms. The characterisation of the biosurfactant using magnetic nuclear resonance and Fourier transform infrared spectroscopy revealed that the biomolecule is a glycolipid with an anionic nature. The results demonstrate that biosurfactant produced in this work has potential as an active biotechnological ingredient for innovative, eco-friendly cosmetic formulations. Full article

Phycocyanin is a natural, non-toxic, blue pigment-protein with many commercial applications. Its exploitation in various biotechnological sectors strongly depends on its purity grade (P). Phycocyanin is largely used in food industry where a low purity grade is required, while its widespread use in sectors requiring a higher purity is hampered by the cost of large-scale industrial production. Industry, in fact, needs simple, easily scalable and cost-effective procedures to ensure sustainable production of high-quality pigment. In this work we applied the innovative two-step ultrasound-based process UltraBlu to the pilot-scale production of phycocyanin. A total of 50 L of biomass suspension of commercial Spirulina were processed in batch mode. The pigment extract was obtained in one day, including the biomass harvesting. Food/cosmetic grade (P = 1.41–1.76) and a good yield (Y = 59.2–76.1%) were achieved. The initial results obtained suggest that UltraBlu can be an effective scalable process suitable to produce phycocyanin also on an industrial scale. Full article

This article presents an empirical evaluation of the technical and economic performance of a building-integrated photovoltaic (PV) system implemented at the Faculty of Architecture and Urbanism of the University of Cuenca, Ecuador. This study explores both stages of deployment, beginning with a 7.7 kWp pilot system and later scaling to a full 75.6 kWp configuration. This hourly monitoring of power exchanges with utility was conducted over several months using high-resolution instrumentation and cloud-based analytics platforms. A detailed comparison between projected energy output, recorded production, and real energy consumption was carried out, revealing how seasonal variability, cloud cover, and academic schedules influence system behavior. The findings also include a comparison between billed and actual electricity prices, as well as an analysis of the system’s payback period under different cost scenarios, including state-subsidized and real-cost frameworks. The results confirm that energy exports are frequent during weekends and that daily generation often exceeds on-site demand on non-working days. Although the university benefits from low electricity tariffs, the system demonstrates financial feasibility when broader public cost structures are considered. This study highlights operational outcomes under real-use conditions and provides insights for scaling distributed generation in institutional settings, with particular relevance for Andean urban contexts with similar solar profiles and tariff structures. Full article

The singular biodiversity of the Brazilian Caatinga inspires innovative solutions in food science. In this study, we evaluated the prebiotic potential of honeys produced by Apis mellifera in the Pajeú hinterland, Pernambuco, Brazil (Caatinga Biome), with different floral origins: Mastic (Aroeira), Mesquite (Algaroba), and mixed flowers. These were used to formulate synbiotic and alcoholic beverages fermented by Saccharomyces boulardii CNCM I-745. Static and dynamic simulations of the human gastrointestinal tract (GIT) were used, as well as physicochemical, rheological, and microbiological analyses. The results revealed that honey positively influences the viability and resilience of probiotic yeast, especially honey with a predominance of Algaroba, which promoted the highest survival rate (>89%) even after 28 days of refrigeration and in dynamic in vitro simulation of the GIT (more realistic to human physio-anatomical conditions). The phenolic composition of the honeys showed a correlation with this tolerance. The use of complementary methodologies, such as flow cytometry, validated the findings and highlighted the functional value of these natural matrices, revealing an even greater longevity potential compared to conventional microbiological methodology. The data reinforces the potential of the Caatinga as a source of bioactive and sustainable compounds, proposing honey as a promising non-dairy synbiotic vehicle. This work contributes to the appreciation of the biome and the development of functional food products with a positive social, economic, and ecological impact. Full article

Skin aging is influenced by intrinsic and extrinsic factors, leading to structural changes in the skin. Current anti-aging cosmetic trends emphasize innovative natural ingredients, including plant extracts, thermal waters, and botanical hydrolats. This work aims to develop six natural anti-aging cosmetics (two serums, two day creams, and two night creams) with innovative and non-irritating profiles. The rational design was guided by market analysis and ingredient properties. Prototype formulations were created with two core mixtures: (1) natural mineral water from Termas de Unhais da Serra and Thymus × citriodorus hydrolat and (2) natural mineral water from Termas de Chaves and aqueous Vaccinium myrtillus (blueberry) extract. The products were evaluated for stability (4 °C and 40 °C, 4 weeks), safety (according to EC Regulation No. 1223/2009), including in vitro testing for skin irritation potential (epiderm model; OECD TG 439). The market study highlighted a gap in anti-aging products combining natural extracts and thermal waters. All prototypes showed desirable textures and remained stable under test conditions. No irritating effects were observed. The results support the development of effective anti-aging cosmetics rooted in natural resources. These innovative products can meet the market demand for natural and sustainable skincare solutions. Full article

This paper proposes a novel mixed game-based day-ahead operation strategy for multi-building integrated energy systems, which innovatively addresses both inter-building cooperation and non-cooperative energy transactions with system operators under uncertainties. Specifically, a bi-level operation model is established in which the upper level maximizes the benefits of the energy system operator, and the lower level minimizes the costs of multiple buildings. Then, in consideration of source-load uncertainties in multiple building energy systems, the fuzzy chance-constrained programming method is introduced, and the clear equivalent class method is used to reformulate the fuzzy chance constrained model into a tractable deterministic type. Further, a privacy-preserving hierarchical solution approach is presented to solve the bi-level optimization model, and the Shapley value method is adopted for benefits redistribution. Case studies on a multi-building system in East China showcase the effectiveness of the proposed work and demonstrate that the proposed strategy contributes to reducing the operation costs of the multi-building system by approximately 3.98% and increasing the revenue of the energy system operators by 10.31%. Full article

Surface all-wave net radiation ($R_n$) is one of the essential parameters to describe surface radiative energy balance, and it is of great significance in scientific research and practical applications. Among various acquisition approaches, the estimation of $R_n$ from satellite data is gaining more and more attention. In order to obtain the daily $R_n$ ($R_{nd}$) from the instantaneous satellite observations, a parameter $C_d$, which is defined as the ratio between the $R_n$ at daily and at instantaneous under clear sky was proposed and has been widely applied. Inspired by the sinusoidal model, a new model for $C_d$ estimation, namely New Model, was proposed based on the comprehensive clear-sky $R_n$ measurements collected from 105 global sites in this study. Compared with existing models, New Model could estimate $C_d$ at any moment during 9:30~14:30 h, only depending on the length of daytime. Against the measurements, New Model was evaluated by validating and comparing it with two popular existing models. The results demonstrated that the $R_{nd}$ obtained by multiplying $C_d$ from New Model had the best accuracy, yielding an overall $R^2$ of 0.95, root mean square error (RMSE) of 14.07 Wm⁻², and Bias of −0.21 Wm⁻². Additionally, New Model performed relatively better over vegetated surfaces than over non- or less-vegetated surfaces with a relative RMSE (rRMSE) of 11.1% and 17.89%, respectively. Afterwards, the New Model $C_d$ estimate was applied with MODIS data to calculate $R_{nd}$. After validation, the $R_{nd}$ computed from $C_d$ was much better than that from the sinusoidal model, especially for the case MODIS transiting only once in a day, with $R_{nd}$-validated $R^2$ of 0.88 and 0.84, RMSEs of 19.60 and 27.70 Wm⁻², and Biases of −0.76 and 8.88 Wm⁻². Finally, more analysis on New Model further pointed out the robustness of this model under various conditions in terms of moments, land cover types, and geolocations, but the model is suggested to be applied at a time scale of 30 min. In summary, although the new $C_d$ model only works for clear-sky, it has the strong potential to be used in estimating $R_{nd}$ from satellite data, especially for those having fine spatial resolution but low temporal resolution. Full article

In this study several phosphorus fertilizers were evaluated under controlled production conditions using Lactuca sativa var. baby leaf and a clay-loam soil of pH 6.5 as a plant–soil model system. Various inorganic (phosphate rock, monoammonium phosphate, struvite), organic (bone meal and bone meal pelletized with compost) and organomineral fertilizers (phosphate rock, monoammonium phosphate, struvite pelletized with compost) were compared. The soil properties, crop yield, morphological aspects and metabolomics of the plants were analyzed. After 45 days of the growing cycle, the organomineral fertilizers (OMFs) composed of compost and monoammonium phosphate (OMF₂(MAP+C)) or struvite (OMF₃(STR+C)) exhibited the best yield results: 101.37 g and 83.21 g, respectively. These treatments also exhibited the best phosphorus use efficiency (PUE) results: 7.40% and 8.33%, respectively. The yield of plants treated with MAP was 56.01 g, and its PUE was 5.33%. The yield of plants treated with STR was 62.10 g and the PUE was 4.67%. Accordingly, the development of OMFs with compost had a positive effect regarding MAP and STR fertilization. Lettuce fertilized with organic bone meal fertilizers had the lowest yield and nutrient use efficiency. The non-targeted metabolic study of green tissue revealed an overactivation of the TriCarboxylic Acids-TCA cycle and amino acid biosynthesis in plants fertilized with bone meal and phosphate rock treatments, likely as a plant stress response. The overall conclusion of this work is that the development of OMFs with compost is a good strategy to increase soil P availability and, accordingly, plant P uptake and %PUE. Full article

Previous research has linked both endothelial protein changes and vitamin D with infertility. This study was undertaken to investigate the association of proteins associated with endothelial function and vitamin D status in the luteal phase at day 21 in a group of non-obese women prior to in vitro fertilization (IVF) with either unexplained infertility (UI) or male factor infertility (MFI). Twenty-five non-obese Caucasian women from a UK academic center with MFI (n = 14) and UI (n = 11) were recruited. Blood was withdrawn at day 21 of the menstrual cycle at the time of mock embryo transfer. Vitamin D parameters were measured by tandem mass spectroscopy. Off-rate Modified Aptamer (SOMA)-scan plasma protein measurement was undertaken for 20 protein markers of endothelial dysfunction. Baseline demographics did not differ between groups and parameters of response following IVF did not differ. Vitamins D₂ and D₃, and 1,25 Vitamin D₃ did not differ between groups. In UI, markers of endothelial activation/dysfunction were investigated; vascular cell adhesion molecule 1 (VCAM-1) decreased and this is associated with endothelial stress; vascular endothelial growth factor (VEGF) decreased and this may suggest impaired endometrial angiogenesis; while intercellular adhesion molecule 1 (ICAM-3) increased (p < 0.05) and is associated with increased immunological activity. A marker of vascular integrity, angiopoietin-1, increased while soluble angiopoietin-1 receptor (sTie-2) decreased (p < 0.05), suggesting increased vascular development. Endothelial markers of inflammation, coagulation, and endothelial progenitor cells were unchanged. Vitamin D and its metabolites show no relationship to UI, but endothelial activation/dysfunction and vascular integrity changes in VCAM-1, VEGF, sICAM-3, angiopoietin-1, and sTie-2 may contribute to UI, though the mechanisms through which they work require further evaluation; however, these protein changes have been associated with endometriosis, raising the suggestion that subclinical/undiagnosed endometriosis may have contributed to UI in these subjects. Full article

In the last decade, magnetic nanoparticles (MNPs) have attracted much attention for the implementation of non-invasive approaches suitable for the diagnosis and treatment of vascular diseases. In this work, the optimization of novel vascular grafts loaded with Nickel-based nanoparticles via electrospinning is proposed. Two different polycarbonate urethanes—i.e., Corethane A80 (COT) and Chronoflex AL80 (CHF)—were used to fabricate 3D electrospun nanocomposite grafts. SEM analysis showed a homogeneous distribution of fibers, with slight differences in terms of average diameters as a function of the polymer used—(1.14 ± 0.18) µm for COT, and (1.33 ± 0.23) µm for CHF—that tend to disappear in the presence of MNPs—(1.26 ± 0.19) µm and (1.26 ± 0.213) µm for COT/NPs and CHF/NPs, respectively. TGA analyses confirmed the higher ability of CHF to entrap MNPs in the fibers—18.25% with respect to 14.63% for COT—while DSC analyses suggested an effect of MNPs on short-range rearrangements of hard/soft micro-domains of CHF. Accordingly, mechanical tests confirmed a decay of mechanical strength in the presence of MNPs with some differences depending on the matrix—from (6.16 ± 0.33) MPa to (4.55 ± 0.2) MPa (COT), and from (3.67 ± 0.18) MPa to (2.97 ± 0.22) MPa (CNF). The in vitro response revealed that the presence of MNPs did not negatively affect cell viability after 7 days in in vitro culture, suggesting a promising use of these materials as smart vascular grafts able to support the actuation function of vessel wall muscles. Full article

The application of non-thermal (cold) plasmas is considered an environmentally friendly method that could affect plant metabolism and cellular development or can be used for the commercial production of natural products that cannot be chemically synthesized. In the present study, the non-embryogenic callus of iris (Iris reichenbachii Heuff.) was treated with a Radio Frequency (RF) plasma needle device using He as a working gas. We investigated short-term (up to seven days) and long-term (up to one year) changes on morphological, physiological and biochemical levels. An increased production of O₂⁻ and H₂O₂ was observed in the callus tissue after plasma treatment. The enzymes SOD and CAT represented the frontline in the antioxidant defense against reactive oxygen species (ROS) produced during the first hour of treatment, while POX was the leading antioxidant enzyme seven days after plasma treatment. Significant long-term morphological changes were observed in the calli due to the increased mitotic activity of the plant cells. In addition, three flavonoids (naringenin, apigenin and acacetin) and two isoflavonoids (irisolidone and irilone) were detected only in the plasma-treated tissue even one year after plasma treatment. The present study emphasizes the application of the plasma technique to promote meristematic activity and stimulate the production of specialized metabolites in iris calli. Full article

Nearshore bathymetry is key to most oceanographic studies and coastal engineering works. This work proposes a new methodology to assess nearshore wave celerity and infer bathymetry from video images. Shoaling and breaking wave patterns were detected on the Timestacks distinctly, and wave celerity was estimated from wave trajectories. The wave type separation enabled the implementation of specific domain formulations for depth inversion: linear for shoaling and non-linear for breaking waves. The technique was validated over a rocky bottom using video acquisition of an online streaming webcam for a period of two days, with significant wave heights varying between 1.7 m and 3.5 m. The results were corroborated in comparison to ground-truth data available up to a depth of 10 m, yielding a mean bias of 0.05 m and a mean root mean square error (RMSE) of 0.43 m. In particular, RMSE was lower than 15% in the outer surf zone, where breaking processes occur. Overall, the depth-normalized RMSE was always lower than 20%, with the major inaccuracy due to some local depressions, which were not resolved. The developed technique can be readily applied to images collected by coastal monitoring stations worldwide and is applicable to drone video acquisitions. Full article

Listeria monocytogenes poses significant risks in acidic foods like unpasteurized fruit juices due to its capacity to survive under stressful conditions. This study evaluated L. monocytogenes survival in orange juice following acid adaptation and exposure to antimicrobial compounds. Acid adaptation was induced using glucose-supplemented or citric acid-acidified media, followed by the evaluation of pathogen survival in orange juice stored at 4 °C, 15 °C, and 25 °C. While glucose adaptation reduced the medium pH to 4.5 and enabled bacterial growth (up to 7.5 total log CFU/mL), citric acid exposure caused around 1.4 log units of reduction. Contrary to expectations, the survival of acid-adapted cells was lower than that of non-acid-adapted cells, particularly in orange juice stored at 25 °C (around 4.8 vs. 1.4 log units of reduction after 6 days). The behaviour of non-acid-adapted cells was evaluated in response to different antimicrobial compounds (citral, coumaric acid, nisin, sinapic acid, and vanillin). Nisin was the most effective, achieving a reduction of about 3.5 log units with a dose of 2 mL/L. Nisin-treated cells also showed reduced survival during simulated gastrointestinal assays (around 1.5 log units of reduction). These results challenge the assumption that acid adaptation universally enhances survival in acidic matrices and highlight nisin’s dual role in microbial control and pathogenicity mitigation. This work underscores the need for tailored stress adaptation studies and natural antimicrobial applications to improve food safety in minimally processed fruit juices. Full article

Background/Objectives: Rho-associated coiled-coil-containing protein kinase inhibitors (ROCKis) have now become known as modulators of corneal endothelial wound repair and cell survival. However, evidence remains fragmented across laboratory and clinical reports. We performed a systematic review to synthesize preclinical and clinical data on ROCKis in corneal disease, assess their efficacy and safety, and identify research gaps. Methods: We searched PubMed, Web of Science, Scopus, and Google Scholar (until May 2025) for English-language original studies evaluating ROCKis in corneal models or patients. Inclusion criteria encompassed in vitro, ex vivo, in vivo, and clinical trials reporting functional outcomes (endothelial cell density, wound closure, visual acuity). Results: Thirty-one studies met criteria: 14 preclinical studies and 17 clinical studies. Preclinical models (rabbit, porcine, human explants) uniformly showed ROCKis (Y-27632, Ripasudil, Netarsudil, H-1152) accelerate corneal endothelial cell proliferation, migration, and restoration of a hexagonal monolayer with improved barrier and pump function over days to weeks. In 17 clinical investigations, topical Ripasudil or Netarsudil and cultured cell injections achieved significant corneal thinning, endothelial cell density and central corneal thickness changes, and visual acuity improvements (≥2 lines) with minimal adverse events. Overall bias was moderate in non-randomized studies and low in the RCTs. Conclusions: ROCKis demonstrate consistent pro-regenerative effects on corneal endothelium in multiple models and show promising clinical efficacy in Fuchs endothelial dystrophy and pseudophakic endothelial failure. Future work should explore novel delivery systems and larger controlled trials to optimize dosing, safety, and long-term outcomes. Full article