Search Results (442)

Titanium alloys such as Ti-6Al-4V are widely used in biomedical implants due to their excellent mechanical properties and biocompatibility, but their bioinert nature limits osseointegration and antibacterial performance. This study proposes a multifunctional surface coating system integrating a thermally oxidized TiO₂ interlayer with a hydroxyapatite (HAp) top layer synthesized via a green route using Hylocereus undatus extract. The HAp was deposited by electrophoretic deposition (EPD), enabling continuous coverage and strong adhesion to the pre-treated Ti-6Al-4V substrate. Structural, morphological, chemical, and electrical characterizations were performed using XRD, SEM, EDS, Raman spectroscopy, and impedance spectroscopy. Bioactivity was assessed through apatite formation in simulated body fluid (SBF), while antibacterial properties were evaluated against Staphylococcus aureus. The results demonstrated successful formation of crystalline TiO₂ (rutile phase) and calcium-rich HAp with good surface coverage. The HAp-coated surfaces exhibited significantly enhanced bioactivity and strong antibacterial performance, likely due to the combined effects of surface roughness and the bioactive compounds present in the plant extract. This study highlights the potential of eco-friendly, bio-inspired surface engineering to improve the biological performance of titanium-based implants. Full article

The development of active food packaging has evolved rapidly in recent years, offering innovative solutions to enhance food preservation and safety while addressing sustainability challenges. This review compiles and analyzes recent advancements (2019–2024) in release-type active packaging, focusing on essential oils, natural extracts, and phenolic compounds as active agents. Primarily plant-derived, these compounds exhibit significant antioxidant and antimicrobial activities, extending shelf life and enhancing food quality. Technological strategies such as encapsulation and polymer blending have been increasingly adopted to overcome challenges related to volatility, solubility, and sensory impact. Integrating bio-based polymers, including chitosan, starch, and polylactic acid, further supports the development of environmentally friendly packaging systems. This review also highlights trends in compound-specific research, release mechanisms, and commercial applications, including a detailed analysis of patents and case studies across various food matrices. These developments have already been translated into practical applications, such as antimicrobial sachets for meat and essential oil-based pads for fresh produce. Moreover, by promoting the valorization of agro-industrial by-products and the use of biodegradable materials, emission-type active packaging contributes to the principles of the circular economy. This comprehensive overview underscores the potential of natural bioactive compounds in advancing sustainable and functional food packaging technologies. Full article

Spice by-products, often discarded as waste, represent an untapped resource for sustainable packaging solutions due to their unique, multifunctional, and bioactive profiles. Unlike typical plant residues, these materials retain diverse phytochemicals—including phenolics, polysaccharides, and other compounds, such as essential oils and vitamins—that exhibit controlled release antimicrobial and antioxidant effects with environmental responsiveness to pH, humidity, and temperature changes. Their distinctive advantage is in preserving volatile bioactives, demonstrating enzyme-inhibiting properties, and maintaining thermal stability during processing. This review encompasses a comprehensive characterization of phytochemicals, an assessment of the re-utilization pathway from waste to active materials, and an investigation of processing methods for transforming by-products into films, coatings, and nanoemulsions through green extraction and packaging film development technologies. It also involves the evaluation of their mechanical strength, barrier performance, controlled release mechanism behavior, and effectiveness of food preservation. Key findings demonstrate that ginger and onion residues significantly enhance antioxidant and antimicrobial properties due to high phenolic acid and sulfur-containing compound concentrations, while cinnamon and garlic waste effectively improve mechanical strength and barrier attributes owing to their dense fiber matrix and bioactive aldehyde content. However, re-using these residues faces challenges, including the long-term storage stability of certain bioactive compounds, mechanical durability during scale-up, natural variability that affects standardization, and cost competitiveness with conventional packaging. Innovative solutions, including encapsulation, nano-reinforcement strategies, intelligent polymeric systems, and agro-biorefinery approaches, show promise for overcoming these barriers. By utilizing these spice by-products, the packaging industry can advance toward a circular bio-economy, depending less on traditional plastics and promoting environmental sustainability in light of growing global population and urbanization trends. Full article

The excessive use of synthetic pesticides has not only resulted in increased resistance among weeds and pests, leading to significant economic loss, but has also raised serious health and environmental concerns. Chalcones and their derivatives, known for their herbicidal, fungicidal, bactericidal, and antiviral properties, are emerging as promising bio-based candidates. These naturally occurring compounds have long been recognized for their beneficial health effects and wide-range applications. However, their limited concentration in plants, along with poor solubility and bioavailability, brings challenges for their development. The aim of this study was to examine the properties of a synthetic substance, pinocembrin dihydrochalcone (3-phenyl-1-(2,4,6-trihydroxyphenyl)-1-propanone), including its soil dissipation and adsorption. Additionally, we evaluated its antioxidant activity through the DPPH assay and FRAP experiments. This analysis aims to provide insights into its potential classification as a low risk pesticide. Full article

Background: Biomimetic strategies have gained increasing attention for their ability to enhance the delivery, stability, and functionality of nutraceuticals by emulating natural biological systems. However, the literature remains fragmented, often focusing on isolated technologies without integrating regulatory, predictive, or translational perspectives. Objective: This review aims to provide a comprehensive and multidisciplinary synthesis of biomimetic and bio-inspired nanocarrier strategies for nutraceutical delivery, while identifying critical gaps in standardization, scalability, and clinical translation. Results: We present a structured classification matrix that maps biomimetic delivery systems by material type, target site, and bioactive compound class. In addition, we analyze predictive design tools (e.g., PBPK modeling and AI-based formulation), regulatory frameworks (e.g., EFSA, FDA, and GSRS), and risk-driven strategies as underexplored levers to accelerate innovation. The review also integrates ethical and environmental considerations, and highlights emerging trends such as multifunctional hybrid systems and green synthesis routes. Conclusions: By bridging scientific, technological, and regulatory domains, this review offers a novel conceptual and translational roadmap to guide the next generation of biomimetic nutraceutical delivery systems. It addresses key bottlenecks and proposes integrative strategies to enhance design precision, safety, and scalability. Full article

The objective of this study was to investigate the chemical composition of the obtained products, as well as the antioxidant activity and bio-stimulant potential of the liquid fractions. The biomass was subjected to pyrolysis in a pilot-scale reactor, followed by distillation of the pyroligneous liquid to separate volatile compounds and enrich bioactive fractions. The samples were analysed by FTIR, TGA/DTG, and GC-MS. Antioxidant activities were assessed through DPPH, FRAP, and total phenolic content assays, while the bio-stimulant potential was evaluated through germination and growth tests of lettuce and arugula seeds. The results showed that the distilled fraction had lower acidity, greater chemical stability, and high antioxidant activity, with the presence of industrially valuable compounds such as methoxylated phenols and furfural. Furthermore, application of the distilled liquid at 0.1% concentration stimulated early seedling development—especially in arugula—while higher concentrations demonstrated inhibitory effects. These findings show that distillation of pyroligneous liquid is an effective strategy to enhance its bioactive properties, enabling its use as a natural antioxidant and plant bio-stimulant. Full article

Bio-based and/or biodegradable food contact materials are being developed as alternatives to conventional petroleum-based materials. Like other food contact materials, these are subject to regulatory requirements. The characterization of these biomaterials enables the identification of chemical substances that could potentially migrate from these materials into food and may pose a risk to consumer health. In this work, commercial samples of food contact materials labeled as bio-based and/or biodegradable were analyzed. To tentatively identify compounds, two analytical methods were optimized: purge and trap (P&T) for volatile compounds and methanolic extract injection for the determination of semi-volatile compounds, both using gas chromatography coupled with mass spectrometry (GC-MS). Compound toxicity was estimated using an in silico methodology, namely Cramer’s rules. More than 200 compounds of different natures were tentatively identified, but only 29 are included in Regulation (EU) 10/2011 on plastic materials intended to come into contact with food, and 38 of them were classified as high-toxicity compounds. Full article

Over the past decade, microbial natural products research has witnessed a transformative “deep-mining era” driven by key technological advances such as high-throughput sequencing (e.g., PacBio HiFi), ultra-sensitive HRMS (resolution ≥ 100,000), and multi-omics synergy. These innovations have shifted discovery from serendipitous isolation to data-driven, targeted mining. These innovations have transitioned discovery from serendipitous isolation to data-driven targeted mining. Genome mining pipelines (e.g., antiSMASH 7.0 and DeepBGC) can now systematically discover hidden biosynthetic gene clusters (BGCs), especially in under-explored taxa. Metabolomics has achieved unprecedented accuracy, enabling researchers to target novel compounds in complex extracts. Integrated strategies—combining genomic prediction, metabolomics analysis, and experimental validation—constitute new paradigms of current “deep mining”. This review provides a systematic overview of 185 novel microbial natural products discovered between 2018 and 2024, and dissects how these technological leaps have reshaped the discovery paradigm from traditional isolation to data-driven mining. Full article

A subject of increasing fundamental and technological interest is the techno- and bio-functionality of functional foods and nutraceuticals in high-solid gels. This encompasses the diffusion of natural bioactive compounds, prevention of oxidation of essential fatty acids, minimization of food browning, and the prevention of malodorous flavour formation in enzymatic and non-enzymatic reactions, to mention but a few. Textural and sensory considerations require that these delivery/encapsulating/entrapping vehicles are made with natural hydrocolloids and co-solutes in a largely amorphous state. It is now understood that the mechanical glass transition temperature is a critical consideration in monitoring the performance of condensed polymer networks that incorporate small bioactive compounds. This review indicates that the metastable properties of the rubber-to-glass transition in condensed gels (as opposed to the thermodynamic equilibrium in crystalline lattices) are a critical parameter in providing a fundamental quality control of end products. It appears that the “sophisticated synthetic polymer research” can provide a guide in the design of advanced biomaterials for targeted release or the prevention of undesirable byproducts. Such knowledge can assist in designing and optimizing functional foods and nutraceuticals, particularly those including vitamins, antioxidants, essential fatty acids, stimulants for performance enhancement, and antimicrobials. Full article

Betalains are hydrophilic natural pigments commonly found in plants of the Caryophyllales order, as well as in specific species and genera of fungi, such as Hygrocybe, Hygrophorus, and Amanita muscaria. Betalains are sorted into two groups: betacyanins, which form red-violet pigments, and betaxanthins, which form yellow-orange pigments. These compounds can be employed as colorimetric sensors and biosensors. This paper provides a review of the isolation methods of betalains and the various applications of betalains as colorimetric sensors and biosensors. The review was conducted by collecting publications over the last decade. The results show that betalains can be used as a colorimetric sensor to identify metal compounds in water and nonmetal compounds that indicate the quality of food. In addition, betaxanthin has been used for developing cell-based biosensors from yeast and bacteria. Furthermore, betalain as a colorimetric sensor and biosensor is developed by using an innovative digital detector, such as a smartphone. Nevertheless, the fragile stability of betalains presents a significant barrier during the extraction. As a result, future studies could focus on adding innovative technologies for optimizing extraction and also developing betalain as novel bio-indicators for specific analytes. Full article

Natural rubber is a widely used biological polymer material because of its excellent comprehensive performance. Nevertheless, the performance of domestic natural rubber cannot meet the requirements for high-end products such as aviation tires, which has become a constraint on the innovation and upgrading of high-end manufacturing enterprises and the enhancement of global competitiveness in China. To solve the bottleneck problem of natural rubber processing technology, this study systematically analyzed the effects of different varieties of fresh latex ratios on the processing and dynamic properties of bio-coagulated natural rubber. By mixing PR107 and Reyan72059 fresh latex with Reyan73397 fresh latex according to proportion, the fresh latex was coagulated by enzyme-assisted microbials, and the effects of the fresh latex ratio on physical and chemical indexes, molecular weight distribution, vulcanization characteristics, processing properties, cross-link density and physical and mechanical properties of the natural rubber were analyzed. The results showed that the aging resistance of natural rubber coagulated with enzyme-assisted microbial decreased, and the aging resistance of natural rubber increased with the increase in the mixing ratio of PR107 and Reyan72059 fresh latex. The proportion of high molecular weight of the natural rubber coagulated with the enzyme-assisted microbial increased, and the fresh latex mixing had little effect on the molecular weight distribution curve. Under the carbon black formulation, the CRI of the enzyme-assisted microbial coagulated natural rubber compound was relatively larger. Under the same strain conditions, the H-3 compound (PR107:Reyan72059:Reyan73397 = 1:1:3) had the best viscoelasticity and the least internal resistance of rubber molecules. In addition, the cross-link density, tensile strength, elongation at break, and tear strength of H-3 vulcanized rubber were the largest, improved by 23.08%, 5.32%, 12.45% and 3.70% compared with the same H-2 vulcanized rubber. In addition, the heat generation performance was reduced by 11.86%, and the wear resistance improved. Full article

The structural and functional characteristics of oil body proteins (OBPs) isolated from hemp, plum, and jujube seeds were systematically investigated, along with their potential application in constructing curcumin-loaded artificial oleosomes (AOs). OBPs were extracted through alkaline extraction coupled with ultrasonic disruption, followed by comprehensive physicochemical characterization using SDS-PAGE, FTIR spectroscopy, fluorescence spectroscopy, and evaluation of particle size, zeta potential, surface hydrophobicity, solubility, thermal stability, and emulsification properties. Plum seed-derived OBPs were found to demonstrate superior emulsifying capacity and solubility, which were attributed to distinctive structural features, including the following: an elevated random coil content (13%), enhanced surface hydrophobicity (21,781 A.U.), reduced particle size (103 nm), and higher zeta potential (−46 mV). These structural advantages were correlated with improved interfacial adsorption capacity and colloidal stability. When employed in AO fabrication, plum seed OBPs produced curcumin-loaded systems exhibiting maximum encapsulation efficiency (92%), minimal droplet size (5.99 μm), and optimal bio-accessibility (50%) compared to their hemp- and jujube-based counterparts. Furthermore, AOs utilizing plum seed OBPs displayed enhanced antioxidant activity and significantly improved stability. The collective findings establish plum seed OBPs as exceptional natural emulsifiers with strong potential for bioactive compound delivery applications. Full article

A creative, nature-based way to solve environmental issues and promote sustainable development could be the cultivation of Pleurotus spp. mushrooms to use the lignocellulosic waste from Medicinal and Aromatic Plants (MAPs). Pleurotus species are characterized by flexibility and biodegradative capacities to generate bioactive compounds with antibacterial, antioxidant, and nutraceutical properties using lignocellulosic substrates. Aromatic plant residues, such as those from lavender, sage, and mint, can improve the resultant mushrooms’ metabolic profiles and act as nutrient-rich substrates. Higher levels of phenols, flavonoids, and terpenoids can be among these enhancements, which could make mushrooms useful as functional foods. This strategy could provide scalable and affordable waste management solutions by utilizing already existing agricultural systems, including mushroom cultivation, during slow times. Incorporating Pleurotus-based systems can help to produce renewable bio-based products, reduce pollution, and improve soil health. This study not only attempts to demonstrate how Pleurotus species may convert industrial and agricultural waste into valuable, bioactive products, reducing waste and promoting ecological remediation in a circular economy, but also to highlight the viability of using natural processes for economic and environmental sustainability. To exploit the potential of this nature-based approach, future research should concentrate on maximizing substrate consumption, scaling these solutions to industrial levels, and guaranteeing regulatory compliance. Full article

Enzymatic browning poses a formidable obstacle to the commercial sustainability of fresh-cut potatoes. Although the synergistic effects of bio-inductive technologies with natural compounds in anti-browning strategies have been observed, their full potential remains underexplored. To fulfill the demand for synergistic approaches in real-world applications, this research elucidates the complementary effects of short-term high-oxygen (HO, 80%) treatment of whole tubers in conjunction with cod peptides (CP, 0.1%) applied to fresh-cut potato slices in mitigating browning. The results demonstrated that the combined treatment (HO + CP) showed superior anti-browning efficacy compared to single treatments (HO or CP) and the untreated group (control). Specifically, peroxidase (POD) and polyphenol oxidase (PPO) activities were suppressed by 55.7% and 35.1%, respectively, under the synergistic treatment compared with the control after 8 days of storage. Meanwhile, increases in the activities of catalase (CAT), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL), along with an approximately 117% increase in total phenolic content, were noted with synergistic treatment. Furthermore, the combined treatment reduced malondialdehyde (MDA) accumulation by 17.5% on day 8. This effect may be attributed to enhanced antioxidant capacity and the preservation of membrane integrity. In summary, this novel strategy provides a practical synergistic solution for the control of enzymatic browning in fresh-cut potatoes. Full article

Antimicrobial resistance (AMR) poses a significant global health threat, largely driven by the overuse and misuse of antibiotics. Methicillin-resistant Staphylococcus aureus (MRSA), a multidrug-resistant pathogen, remains a critical target for novel antibiotic development. This study explores the rhizosphere of the wild apple tree (Malus trilobata) in Lebanon as a potential source of antibacterial compounds. A bacterial strain, MR7S4, identified as Streptomyces anulatus, was isolated and characterized. Its crude extracts exhibited potent activity against Gram-positive pathogens, with minimum inhibitory concentration (MIC) values of 2 µg/mL against S. aureus ATCC 29213, S. aureus Newman, and S. aureus N315 (MRSA), and of 1 µg/mL against Enterococcus faecalis ATCC 19433. Bio-guided fractionation and structural analysis identified a novel antibacterial pyrazinone derivative, MR7S4-F3. This compound demonstrated MIC values of 4–16 µg/mL against Bacillus subtilis ATCC 6633, multiple S. aureus strains, E. faecalis ATCC 19433, E. faecium DSM 17050 (VRE), and E. faecium DSM 20478, while exhibiting no activity against Gram-negative bacteria. Whole-genome sequencing of MR7S4 revealed 35 biosynthetic gene clusters, underscoring its potential for natural product discovery. These findings highlight the untapped microbial diversity of the Middle East and North Africa (MENA) region as a valuable reservoir for antibiotic discovery. MR7S4-F3 emerges as a promising bioactive scaffold, addressing the urgent need for new therapeutic options to combat AMR. Full article